Aspen Chromatography Aspen Chromatography Hydrophobic Interaction Chromatography (HIC)Hydrophobic Interaction Chromatography (HIC)Chromatography (HIC)Chromatography (HIC)

Bioprocess LaboratoryDepartment of Chemical Engineering

Ch N ti l U i itChungnam National University

Introduction (HIC experimental data)(HIC experimental data)

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Simulation conditions

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Aspen chromatography i l ti simulation The step 1 (Loading step)The step 1 (Loading step)

Time : 10 min

The step 2 (Gradient step)Time : 15 min

The step 3 (Washing step)The step 3 (Washing step)Time : 30 min

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Buffer SimulationBuffer Simulation

Loading stepLoading step

Gradient step

washing step

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NP값변화NP값변화

IP1(Lac-Trn-Tsh) : 10-15-20IP2 : 30IP3 : 8

IP1(Lac-Trn-Tsh) : 10-15-20IP2 : 30IP3 : 8

IP1(Lac-Trn-Tsh) : 10-15-20IP2 : 30IP3 : 8

IP1(Lac-Trn-Tsh) : 10-15-20IP2 : 30IP3 : 8IP3 : 8

NP : 30MTC : 100000

IP3 : 8NP : 300MTC : 100000

IP3 : 8NP : 30000MTC : 100000

IP3 : 8NP : 3000000MTC : 100000

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MTC값변화MTC값변화

IP1(Lac-Trn-Tsh) : 10-15-20IP2 : 30

IP1(Lac-Trn-Tsh) : 10-15-20IP2 : 30

IP3 : 8NP : 30MTC : 1000

IP3 : 8NP : 30MTC : 100000

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값변화IP2값변화

IP1(Lac-Trn-Tsh) : 10-15-20IP2 : 1IP3 : 8

IP1(Lac-Trn-Tsh) : 10-15-20IP2 : 30IP3 : 8

IP1(Lac-Trn-Tsh) : 10-15-20IP2 : 300IP3 : 8

NP : 30MTC : 100000

NP : 30MTC : 100000

NP : 30MTC : 100000

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IP3값변화IP3값변화

IP1(Lac-Trn-Tsh) : 10-15-20IP2 : 30IP3 : 1

IP1(Lac-Trn-Tsh) : 10-15-20IP2 : 30IP3 : 8

IP1(Lac-Trn-Tsh) : 10-15-20IP2 : 30IP3 : 20

NP : 30MTC : 100000

NP : 30MTC : 100000

NP : 30MTC : 100000

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Q값변화Q값변화

Q = 0.15Q = 0.015 Q = 1.5

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Q = 15 Q = 1500

HIC Simulation Interim ResultHIC Simulation Interim Result

IP1(L T T h) : 10 15 20IP1(Lac-Trn-Tsh) : 10-15-20IP2 : 30IP3 : 8NP 30NP : 30MTC : 100000Q : 0.15

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분자량의 변화분자량의 변화

MW(molecular weight)

A, B, C = 10,000

MW(molecular weight)

A = 40,000B = 71,400C = 36,100

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HIC Simulation and Experiment

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