BioLogic DuoFlow Instruction Manual

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<p>BioFrac Franction Collector1 4 7 F1 F2 F3 F4 F5 2 5 8 0 3 6 9</p> <p>A</p> <p>B</p> <p>VALVE A</p> <p>VALVE B</p> <p>B IO L OGIC D UO F LOWCHROMATOGRAPHY SYSTEMINSTRUCTION MANUAL (BioLogic DuoFlow Software Version 5.0)</p> <p>Copyright (2003) Bio-Rad Laboratories, Inc. All rights reserved.</p> <p>TABLE OF CONTENTS</p> <p>TABLE OF CONTENTS</p> <p>SAFETY SECTION 1. Chapter 1.0 1.1 1.2 1.3 1.4 1.5 SYSTEM OVERVIEW Introduction ...............................................................................................................1-1 Overview .....................................................................................................................1-1 Features ......................................................................................................................1-2 Unpacking ...................................................................................................................1-3 System Configurations ................................................................................................1-4 Quick Start Procedure.................................................................................................1-5</p> <p>Chapter 2.0 Description of System Components .......................................................................2-1 2.1 Controller and USB Bitbus Communicator .................................................................2-2 2.1.1 Controller........................................................................................................2-2 2.1.2 USB Bitbus Communicator ............................................................................2-5 2.2 Workstation .................................................................................................................2-6 2.3 BioLogic Maximizer Valve System ..........................................................................2-11 2.4 Mixers..........................................................................................................................2-18 2.4.1 MX-1 Mixer.....................................................................................................2-18 2.4.2 Maximizer Mixer .............................................................................................2-19 2.4.3 Changing Mixer Capacity ...............................................................................2-20 2.5 Detection Systems ......................................................................................................2-21 2.5.1 UV Detector....................................................................................................2-21 2.5.2 Conductivity Monitor.......................................................................................2-23 2.5.3 BioLogic QuadTec UV/Vis Detector............................................................2-23 2.5.4 pH Monitor......................................................................................................2-25 2.5.5 Other Detectors..............................................................................................2-25 2.6 Valves..........................................................................................................................2-26 2.6.1 AVR7-3 Sample Inject Valve ..........................................................................2-26 2.6.2 AVR9-8 Stream Select Valve .........................................................................2-29 2.6.3 SV5-4 Buffer Select and Automated Sample Loading Valve .........................2-31 2.6.4 SVT3-2 Diverter Valve....................................................................................2-33 2.7 Fraction Collectors ......................................................................................................2-35 2.7.1 BioFrac Fraction Collector ..........................................................................2-35 2.7.2 Model 2110 Fraction Collector .......................................................................2-37 2.7.3 Model 2128 Fraction Collector .......................................................................2-38 2.7.4 Generic Fraction Collectors............................................................................2-39 2.8 Sample Loading Options.............................................................................................2-40 2.8.1 DynaLoop Large Volume Sample Injection Loop...........................................2-40 2.8.2 Model EP-1 Econo Pump...............................................................................2-42 2.8.3 EGP Econo Gradient Pump ...........................................................................2-43 2.8.4 Other Gradient Pumps ...................................................................................2-44 2.9 System Peripherals .....................................................................................................2-45 2.9.1 System Rack ..................................................................................................2-45 2.9.2 Starter Kit .......................................................................................................2-47 2.9.3 Fittings Kit, including Tubing Kit .....................................................................2-47 2.9.4 Fittings Tightener............................................................................................2-48 2.9.5 Backpressure Regulator.................................................................................2-49 2.9.6 Signal Import Module (SIM) ...........................................................................2-50 2.9.7 Pump Kits.......................................................................................................2-51 iii</p> <p>TABLE OF CONTENTS</p> <p>2.9.8 Model 1327 Chart Recorder...........................................................................2-52 2.9.9 Generic Chart Recorders ...............................................................................2-52 2.9.10 Uninterruptible Power Supply.........................................................................2-53 2.9.11 Printers ...........................................................................................................2-53 2.10 Columns and Column Fittings.....................................................................................2-54 2.10.1 Anion Exchange: Q-Strong Anion Exchange ................................................2-54 2.10.2 Cation Exchange: S-Strong Cation Exchange..............................................2-54 2.10.3 Anion Exchange: DEAE Weak Anion Exchange...........................................2-55 2.10.4 Cation Exchange: Carboxy Methyl (CM) Weak Cation Exchange ................2-55 2.10.5 Ceramic Hydroxyapatite (CHT) ......................................................................2-55 2.10.6 Size Exclusion Chromatography (SEC) .........................................................2-56 2.10.7 High Pressure Reversed Phase Columns .....................................................2-56 2.10.8 Hydrophobic Interaction Chromatography (HIC)............................................2-56 2.10.9 Affinity Chromatography.................................................................................2-57 2.10.10 Empty Columns..............................................................................................2-57 2.10.11 Column Fittings ..............................................................................................2-58 SECTION 2. Chapter 3.0 3.1 3.2 3.3 3.4 3.5 3.6 SYSTEM INSTALLATION AND SETUP System Setup ............................................................................................................3-1 Controller Cable Connections .....................................................................................3-2 USB Bitbus Communicator Cable Connections..........................................................3-3 Workstation Cable Connections..................................................................................3-4 3.3.1 Systems without a Maximizer ........................................................................3-4 3.3.2 Systems with a Maximizer .............................................................................3-5 System Rack Setup.....................................................................................................3-6 Mixers..........................................................................................................................3-7 Detection System Connections ..................................................................................3-9 3.6.1 UV Detector and Conductivity Monitor...........................................................3-9 3.6.2 QuadTec UV/Vis Detector ..............................................................................3-10 3.6.3 pH Monitor......................................................................................................3-12 3.6.4 Non-Bio-Rad Detectors ..................................................................................3-13 Valve Connections .....................................................................................................3-14 Fraction Collector Connections ..................................................................................3-15 3.8.1 BioFrac Fraction Collector..............................................................................3-15 3.8.2 Model 2110 Fraction Collector .......................................................................3-15 3.8.3 Model 2128 Fraction Collector .......................................................................3-15 Pump Connections .....................................................................................................3-16 3.9.1 Model EP-1 Econo Pump...............................................................................3-16 3.9.2 Econo Gradient Pump (EGP).........................................................................3-17 Model 1327 Chart Recorder Connections ..................................................................3-19 Completing System Setup...........................................................................................3-19 3.11.1 DuoFlow System Network Connections.........................................................3-19 3.11.2 System Power Up ..........................................................................................3-19 3.11.3 BioLogic Configuration Utility Software..........................................................3-20 System Plumbing ......................................................................................................4-1 General Guidelines for Creating Your Own Tubing Connections................................4-2 Plumbing a DuoFlow System......................................................................................4-3 Priming the System .....................................................................................................4-8</p> <p>3.7 3.8</p> <p>3.9 3.10 3.11</p> <p>Chapter 4.0 4.1 4.2 4.3</p> <p>iv</p> <p>TABLE OF CONTENTS</p> <p>SECTION 3. Chapter 5.0 5.1 5.2 5.3</p> <p>SYSTEM OPERATION Introduction to the System Software ......................................................................5-1 System Interface .........................................................................................................5-1 Standard Mouse and Keyboard Functions .................................................................5-2 System Menus ............................................................................................................5-3 5.3.1 Toolbar Buttons ..............................................................................................5-3 5.3.2 Drop-down Menus..........................................................................................5-5 Introduction to the Browser Screen........................................................................6-1 Overview .....................................................................................................................6-1 Method Templates .......................................................................................................6-7 Creating and Running a Queue ..................................................................................6-9 Creating and Viewing a Compare ...............................................................................6-11 Trace Compare ...........................................................................................................6-12 6.5.1 Chromatogram Display Screen ......................................................................6-13 6.5.2 Toolbar Buttons ..............................................................................................6-14 6.5.3 Drop-down Menus..........................................................................................6-15 6.5.4 Active Traces and Valves at Cursor ...............................................................6-17 6.5.5 Chromatogram Settings Tab ..........................................................................6-17</p> <p>Chapter 6.0 6.1 6.2 6.3 6.4 6.5</p> <p>Chapter 7.0 Modes of Operation...................................................................................................7-1 7.1 Manual Screen ............................................................................................................7-2 7.2 Setup Screen ..............................................................................................................7-4 7.2.1 Device Selection ............................................................................................7-5 7.2.2 Inlet and Valve Naming ..................................................................................7-6 7.2.3 Buffer Editor ...................................................................................................7-8 7.3 Protocol Screen...........................................................................................................7-10 7.4 Run Screen .................................................................................................................7-30 7.4.1 Pausing/Stopping a Method in Progress........................................................7-33 7.4.2 Working Offline During a Run ........................................................................7-34 7.4.3 Editing a Method During a Run......................................................................7-35 7.4.4 Run Notebook Screen....................................................................................7-37 7.4.5 Run Log Screen .............................................................................................7-37 7.5 Post Run Screen .........................................................................................................7-38 7.5.1 Resizing.........................................................................................</p>

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