BioLogic DuoFlow Instruction Manual

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B IO L OGIC D UO F LOWCHROMATOGRAPHY SYSTEMINSTRUCTION MANUAL (BioLogic DuoFlow Software Version 5.0)

Copyright (2003) Bio-Rad Laboratories, Inc. All rights reserved.

TABLE OF CONTENTS

TABLE OF CONTENTS

SAFETY SECTION 1. Chapter 1.0 1.1 1.2 1.3 1.4 1.5 SYSTEM OVERVIEW Introduction ...............................................................................................................1-1 Overview .....................................................................................................................1-1 Features ......................................................................................................................1-2 Unpacking ...................................................................................................................1-3 System Configurations ................................................................................................1-4 Quick Start Procedure.................................................................................................1-5

Chapter 2.0 Description of System Components .......................................................................2-1 2.1 Controller and USB Bitbus Communicator .................................................................2-2 2.1.1 Controller........................................................................................................2-2 2.1.2 USB Bitbus Communicator ............................................................................2-5 2.2 Workstation .................................................................................................................2-6 2.3 BioLogic Maximizer Valve System ..........................................................................2-11 2.4 Mixers..........................................................................................................................2-18 2.4.1 MX-1 Mixer.....................................................................................................2-18 2.4.2 Maximizer Mixer .............................................................................................2-19 2.4.3 Changing Mixer Capacity ...............................................................................2-20 2.5 Detection Systems ......................................................................................................2-21 2.5.1 UV Detector....................................................................................................2-21 2.5.2 Conductivity Monitor.......................................................................................2-23 2.5.3 BioLogic QuadTec UV/Vis Detector............................................................2-23 2.5.4 pH Monitor......................................................................................................2-25 2.5.5 Other Detectors..............................................................................................2-25 2.6 Valves..........................................................................................................................2-26 2.6.1 AVR7-3 Sample Inject Valve ..........................................................................2-26 2.6.2 AVR9-8 Stream Select Valve .........................................................................2-29 2.6.3 SV5-4 Buffer Select and Automated Sample Loading Valve .........................2-31 2.6.4 SVT3-2 Diverter Valve....................................................................................2-33 2.7 Fraction Collectors ......................................................................................................2-35 2.7.1 BioFrac Fraction Collector ..........................................................................2-35 2.7.2 Model 2110 Fraction Collector .......................................................................2-37 2.7.3 Model 2128 Fraction Collector .......................................................................2-38 2.7.4 Generic Fraction Collectors............................................................................2-39 2.8 Sample Loading Options.............................................................................................2-40 2.8.1 DynaLoop Large Volume Sample Injection Loop...........................................2-40 2.8.2 Model EP-1 Econo Pump...............................................................................2-42 2.8.3 EGP Econo Gradient Pump ...........................................................................2-43 2.8.4 Other Gradient Pumps ...................................................................................2-44 2.9 System Peripherals .....................................................................................................2-45 2.9.1 System Rack ..................................................................................................2-45 2.9.2 Starter Kit .......................................................................................................2-47 2.9.3 Fittings Kit, including Tubing Kit .....................................................................2-47 2.9.4 Fittings Tightener............................................................................................2-48 2.9.5 Backpressure Regulator.................................................................................2-49 2.9.6 Signal Import Module (SIM) ...........................................................................2-50 2.9.7 Pump Kits.......................................................................................................2-51 iii

TABLE OF CONTENTS

2.9.8 Model 1327 Chart Recorder...........................................................................2-52 2.9.9 Generic Chart Recorders ...............................................................................2-52 2.9.10 Uninterruptible Power Supply.........................................................................2-53 2.9.11 Printers ...........................................................................................................2-53 2.10 Columns and Column Fittings.....................................................................................2-54 2.10.1 Anion Exchange: Q-Strong Anion Exchange ................................................2-54 2.10.2 Cation Exchange: S-Strong Cation Exchange..............................................2-54 2.10.3 Anion Exchange: DEAE Weak Anion Exchange...........................................2-55 2.10.4 Cation Exchange: Carboxy Methyl (CM) Weak Cation Exchange ................2-55 2.10.5 Ceramic Hydroxyapatite (CHT) ......................................................................2-55 2.10.6 Size Exclusion Chromatography (SEC) .........................................................2-56 2.10.7 High Pressure Reversed Phase Columns .....................................................2-56 2.10.8 Hydrophobic Interaction Chromatography (HIC)............................................2-56 2.10.9 Affinity Chromatography.................................................................................2-57 2.10.10 Empty Columns..............................................................................................2-57 2.10.11 Column Fittings ..............................................................................................2-58 SECTION 2. Chapter 3.0 3.1 3.2 3.3 3.4 3.5 3.6 SYSTEM INSTALLATION AND SETUP System Setup ............................................................................................................3-1 Controller Cable Connections .....................................................................................3-2 USB Bitbus Communicator Cable Connections..........................................................3-3 Workstation Cable Connections..................................................................................3-4 3.3.1 Systems without a Maximizer ........................................................................3-4 3.3.2 Systems with a Maximizer .............................................................................3-5 System Rack Setup.....................................................................................................3-6 Mixers..........................................................................................................................3-7 Detection System Connections ..................................................................................3-9 3.6.1 UV Detector and Conductivity Monitor...........................................................3-9 3.6.2 QuadTec UV/Vis Detector ..............................................................................3-10 3.6.3 pH Monitor......................................................................................................3-12 3.6.4 Non-Bio-Rad Detectors ..................................................................................3-13 Valve Connections .....................................................................................................3-14 Fraction Collector Connections ..................................................................................3-15 3.8.1 BioFrac Fraction Collector..............................................................................3-15 3.8.2 Model 2110 Fraction Collector .......................................................................3-15 3.8.3 Model 2128 Fraction Collector .......................................................................3-15 Pump Connections .....................................................................................................3-16 3.9.1 Model EP-1 Econo Pump...............................................................................3-16 3.9.2 Econo Gradient Pump (EGP)

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