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Applied Analytical Chemistry

Annual Report 2018

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Table of Contents

Applied Analytical Chemistry ................................................................................................................... 4

Applied Analytical Chemistry Staff ....................................................................................................... 6

Major News 2018 ..................................................................................................................................... 7

Hero of the Year 2018 ............................................................................................................................. 9

List of Projects 2018 .............................................................................................................................. 10

Effect-based analysis ............................................................................................................................ 12

Analysis of complex samples using multidimensional separation and detection techniques .............. 13

At column dilution (ACD) modulator developing for flexible and precise control dilution factors to overcome mobile phase incompatibility in comprehensive two-dimensional liquid chromatography ..................................................................................................................................... 14

µLC+LC-IM-qTOF-MS for a four-dimensional proteome analysis ......................................................... 15

Optimization of SPE protocols for the enrichment of phenolic compounds .......................................... 16

Characterization of the plasma lipidome using LC-IM-qTOF-MS/MS ................................................... 17

Lipidomic profiling of pancreatic cancer cells and corresponding liver metastases .............................. 18

Analysis of complex natural substances: Marijuana and Cannabinoids with GC+GC-APCI-IM-MS ......................................................................................................................................................... 19

Characterization of the metabolome of P. aeruginosa in biofilm as a lung infection model .................. 20

Thermogravimetry atmospheric pressure photoionization mass spectrometry (TG-APPI-MS) as analytical tool for the analysis of pharmaceutical tablets ...................................................................... 21

A modern concept for regulatory water monitoring via High-Performance Liquid Chromatography coupled to high-resolution mass spectrometry or how less can be more ................. 22

Development of an ESI and APCI dual ionization source ..................................................................... 23

Molecular Evolution in a Peptide-Vesicle System ................................................................................. 24

Ozone Stress Effect on the Intracellular Metabolites from Cobetia marina measured by GCxGC-MS ......................................................................................................................................................... 25

How to deal with mercury in sediments? A short summary about the aspects of mercury speciation in sediments. ........................................................................................................................ 26

New developments in chemical ionization at atmospheric pressure ..................................................... 27

Capillary zone electrophoresis coupled to drift tube ion mobility-mass spectrometry for the analysis of native and APTS-labeled N-glycans.................................................................................... 28

Doctoral Theses accomplished 2018 .................................................................................................... 29

Master Theses accomplished 2018 ....................................................................................................... 30

Bachelor Theses accomplished 2018 ................................................................................................... 30

Accepted and/or Published Scientific Publications 2018 ...................................................................... 31

Invited Lectures / Oral Presentations .................................................................................................... 33

Miscellaneous ........................................................................................................................................ 35

Institute Colloquium ............................................................................................................................... 37

Teaching ................................................................................................................................................ 38

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Annual Report 2018

of 40

List of Projects 2018

(Abstracts of these projects within the next pages)

Effect-based analysis

Sven W. Meckelmann, Julia Klein, Martin Meyer

Analysis of complex samples using multidimensional separation

and detection techniques

Julia Klein

At column dilution (ACD) modulator developing for flexible and precise control

dilution factors to overcome mobile phase incompatibility in comprehensive two-

dimensional liquid chromatography

Yingzhuang Chen, Junjie Li

µLC+LC-IM-qTOF-MS for a four-dimensional proteome analysis

Lidia Montero

Optimization of SPE protocols for the enrichment of phenolic compounds

Martin Meyer

Characterization of the plasma lipidome using LC-IM-qTOF-MS/MS

Kristina Rentmeister, Sven W. Meckelmann

Lipidomic profiling of pancreatic cancer cells and

corresponding liver metastases

Sven W. Meckelmann

Analysis of complex natural substances:

Marijuana and Cannabinoids with GC+GC-APCI-IM-MS

Christian Lipok

Characterization of the metabolome of P. aeruginosa in biofilm

as a lung infection model

Timo Koehler

Thermogravimetry atmospheric pressure photoionization mass spectrometry

(TG-APPI-MS) as analytical tool for the analysis of pharmaceutical tablets

Dominik Brecht, Florian Uteschil

A modern concept for regulatory water monitoring via High-Performance Liquid

Chromatography coupled to high-resolution mass spectrometry

or how less can be more

Susanne Brüggen


Annual Report 2018

of 40

Development of an ESI and APCI dual ionization source


Molecular Evolution in a Peptide-Vesicle System

Amela Bronja

Ozone Stress Effect on the Intracellular Metabolites from Cobetia marina

measured by GCxGC-MS

Junjie Li

How to deal with mercury in sediments?

A short summary about the aspects of mercury speciation in sediments.

Claudia Hellmann

New developments in chemical ionization at atmospheric pressure

Christian Lipok

Capillary zone electrophoresis coupled to drift tube ion mobility-mass spectrometry

for the analysis of native and APTS-labeled N-glycans

Julia Klein, Sven Meckelmann

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f 40

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Figure: A) SeparµLC+LC-B) Ion msame m/z

f 40

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________

Collabora

f 40

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are at least0 lipids estid in the bigc lipids, why (IMS) is abaric compoed separatid using a dipids in com

using a thrquid chromaa long 60propanol on

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____

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cterization o

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drift-time IMmplex biolog

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– Project Partn

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mass spectra)/MS acquisiti

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d Analytical Ch

nual Report 2

ma lipidom

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018

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________

Collabora

f 40

diagnosed urvive the fiOnly in latertime the cadevelopmenasis is yet p

cently startg group Ba

nd comparemouse mo

as well as frther grown ere extracte

OF-MS.

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Heat map of ing cells fromLS-DA analysf the primary

____

ative Project –

Lipidomic

with pancrrst year, ber stages, syancer has ont of metasoorly under

ted researcrbara Grüned these wit

odel establisrom the corin culture to

ed and analy

ained raw daction, the variate andrious lipid c

the obtained m a liver metasis of the datatumor cells a

– Project Partn

Applied

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c profiling

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LC-IM-qTOFastases. a after featureand the metas

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d Analytical Ch

nual Report 2

of pancrea

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W. Meckel

er have a vre are usuare specific ed into othere liver. How

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ra Grüner (Mo

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018

atic cancer

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________

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Figure 1GC+GC-

Figure 2m/z 311(CBND-C

f 40

M

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___

by: Internation

. Analyse of aIM-qTOF-MS.

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d Analytical Ch

nual Report 2

mplex natu

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Christian Lip

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Annual Report 2018

of 40

Characterization of the metabolome of P. aeruginosa in biofilm

as a lung infection model

Timo Koehler

Pseudomonas aeruginosa is an opportunistic pathogenic germ, which leads to nosocomial infection. Especially the lung of cystic fibrosis patients is mostly colonized by this bacterium, in form of a biofilm, and leads to fatal lung infections, which causes the early death of cystic fibrosis patients due to raspatory failures. The expected life time of cystic fibrosis patient is increased from about 8 years in 1974 to about 40 years nowadays but this was achieved by an intensive treatment with antibiotics. The drawback of this treatment is the increasing re-sistance of the bacterium against the antibiotics. To reduce the therapy with antibiotics and allow another extension of increase the life time of cystic fibrosis patients a non-invasive ear-

ly detection technique is necessary, but still not developed.

In this project such a detection technique should be developed. Therefore, a biofilm system under adapted lung conditions was planned and tested. This three-phase ap-proach, shown in the left figure, is a biofilm system, where the metabolites are going to be sampled using thin film microextraction (TFME) with PDMS films. The advantage of this model is that in addition to the metabo-

lites of the biofilm, the substances of the nutrient and the headspace can be collected and analyzed. This allows a differentiation of the detected and characterized substances in sub-strates and metabolites.

In the first step of the project, a suitable film material and manufacturer was selected. Thereafter, the cleaning process for the selected film was optimized with the aim of minimal initial contamination. A suitable separation and detection technique for the loaded films, using a thermo desorption system (TDS A2, Gerstel GmbH, Muelheim, Germany) with a GC-MS (Agilent Technologies Inc., Santa Clara, USA) was developed. The development of the TD-GC-MS method was carried out using an aqueous solution consist of twelve standards. The standards were potential metabolites of P. aeruginosa and are already published in scientific journals. With the developed method we are able to identify and quantify several of these possible metabolites down to nanomolar concentrations in a matrix of the nutrient. Furthermore first TFME experiments using liquid cultures of three different strains of P. aeruginosa shows that several metabolites, produced by P. aeruginosa can be detected with the developed method. At the moment we are optimising the developed method with the aim of lower LODs for the potential metabolites and higher peak capacity.

___________

Collaborative Project – Project Partner: PD Dr. Ursula Telgheder (UDE) and Dr. Jost Wingender (UDE)

Funded by: German Research Foundation (DFG), Bonn, Germany

Culture medium

Biofilm

Foils

Figure: schematic overview of the biofilm system for P. aeruginosa as lung infection model.


Annual Report 2018

of 40

Thermogravimetry atmospheric pressure photoionization mass spectrometry

(TG-APPI-MS) as analytical tool for the analysis of pharmaceutical tablets


The reliability of pharmaceutical formulations is an important issue in the industrial and even more important in developing countries. Therefore, a trusted analysis of produced tablets or suspected falsified drugs is very important. At the moment the product control in the industry and the investigation of falsified drugs is done by DSC analysis which only gives the information of physical properties like the melting or boiling point of an analyte. Hyphenated techniques like GC-MS and LC-MS are other methods which are used for the analysis of pharmaceuticals. These methods go hand in hand with a long sample preparation like the extraction of the analytes from the tablet matrix and in some cases also require derivatization. In the analysis of tablets described before the TG-APPI-MS shows an advantage in the sample preparation which is very easy and consists of grinding the tablet to a homogeneous powder. The use of the TG-APPI-MS simplifies the analysis of tablets. When using an APPI-MS as a detector for the evolved gas of the thermogravimetry additional information about the molecule masses are required. This is caused by the soft ionization of the APPI. Additionally, the TG is often equipped with a DTA analysis which also can increase the knowledge about the physical properties of the sample.

The presented figure shows the information which can be collected by the analysis of an acetylsalicylic acid (ASA) tablet by the TG-APPI-MS. Figure A is the TG analysis of 4 mg of the ground tablet. It shows a sharp peak of the DTA signal at 132 °C which is the melting point of the acetylsalicylic acid in the formulation with the excipients. The melting point of the pure ASA is 136 °C. One excipient can be detected which is shown in figure B. Here a signal for the m/z 76 presents the

protonated molecule of glycine. So, the TG-APPI-MS can identify excipients in the pharmaceutical formulations. Also, the fragmentation of acetylsalicylic acid is observed with the m/z values of 121 and 163. These abilities to identify the active substance as well as the excipients, makes the TG-APPI-MS a powerful instrument for the product control and identification of falsified drugs with less sample preparation.

_________

Funded by: Hitachi High-Tech Corporation, Mito, Japan

Figure: A: Thermogravimetric analysis of an acetylsalicylic acid tab-let with the TG-APPI-MS; B: Mass spectrum averaged over the whole analysis time.

50 100 150 200 250 300

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Annual Report 2018

of 40

Development of an ESI and APCI dual ionization source


The demand on high throughput methods for LC-MS is a growing market in the analytical chemistry. Therefore, our group is working on solutions to increase the sample throughput in chromatographic analyses hyphenated with mass spectrometer. Here, we focused our research on the development of new ion sources for mass spectrometry. In this work an ion source should be created which is capable to ionize the analyte molecules with electrospray ionization (ESI) or atmospheric pressure chemical ionization (APCI). Our first attempt to realize such a source is to combine the ESI and the APCI probe in one ion source housing with two entrances for the effluent of the HPLCs. The high sample throughput is realized by connecting two HPLCs with a six-port switching valve which switches between the two HPLCs and the two probes of the ion source. The challenge of this project is to create an ion source which is comparable to single probe ion sources, but compromises must be made because ESI and APCI ionization relies on different ionization mechanism. ESI ionization takes place in the liquid phase and APCI in the gas phase, therefore, there is a challenge to find the right temperature to use both probes as efficient as it is possible. This new ion source shall reduce the delay time of a mass spectrometer to increase the efficiency of mass spectrometric analyses.

The figure presents one of the first analysis with the new developed dual probe ion source. One sample of caffeine (50 ppb) is injected in HPLC 2 and one sample of reserpine (50 ppb) is injected in HPLC 1. Both HPLCs are started at the same time. The chromato-gram is divided in two parts the first is the detection of caffeine using the APCI probe (HPLC 2). After that the six-port valve switches between the two probes and the effluent of the HPLC 1 is directed into the ESI probe. Reserpine and caffeine are detected as protonated molecule ion with a mass of 609 Da and

195 Da, respectively. The first experiments show promising results as it presented here. Especially the short equilibration time of the ESI ion source after the switching of the valve supports a high throughput method, in which analyses can be done by ESI or APCI in one LC run.

_________

Funded by: Hitachi High-Tech Corporation, Mito, Japan

0 50 100 150 200

0,0

2,0x105

4,0x105

6,0x105

8,0x105

1,0x106

ESI

reserpine

Inte

nsity

[cp

s]

RT [s]

caffeine

APCI

Figure: Chromatographic analyses of a caffeine solution (HPLC 2) and a reserpine solution (HPLC 1) by the ESI-APCI- dual probe ion source.

of

Based system proposecyclic psupplyinthe pep

will evethe bilayto simupressurIn ordervesiclespressurpressuris inducducts frlution wLC-qTO

Figure EIC's opeptide amino GASDDGGSDESLLLKKdrophilicphobic have thamphiply, this accumuof mem

________

Collabora

Figure 1lation in

f 40

on the moundergoin

ed. This sysprocesses, ong peptidesptides by th

ntually be eyer membra

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OF-MS.

2 is showof a possib

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peptide hulated and pbrane vesic

____

ative Project –

: Mechanismthe presence

Molecula

del "origin g structurastem is beinone of whics from a varhe vesicles

eluted by scane and remtions given quilibrium aprate the peerature insitedly switchphase transsolid pro-ueous so-yzed with

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possible binations: GGTDDE,

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– Project Partn

of peptide see of vesicles.

Applied

An

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pparatus coptide formaide the cell hed betweesition from s

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d Analytical Ch

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n in a Pept

Amela Bronj

deep-rechinction, self-ounder early esicles as tno acids. Te vesicles b

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018

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hristian Mayer

eptide with th

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possible sces can be ede peptidec amino aciittle interacn in the aundergo hyptide chainso acids (redr) will accumand elution. arth's crustino acids waon pressure

ole experime0 min. DuriCO2 and vic

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________

Collabora

Funded b

f 40

Ozone Stre

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treatment, ka marina ineffect. Afterng to the or

piking, as shone stress ed patternscould be

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Contour plots) spiked with

____

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by: Mercator R

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Gram-negaesearch oveem in mariarely focusecellular proc

known as a different e

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– Project Partn

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a sufficient dextent. Diffe, the deriva formed by

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ner: Profs. T. S

ter Ruhr Gmb

d Analytical Ch

nual Report 2

racellular M

red by GCx

Junjie Li

e bacteriumdecades haar circumsta

abolites asp

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mber of metsage. As cat the majostate one. sappeared yrrolidionond Myristic a

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H (MERCUR)

hemistry

018

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method, haunt of ozonples were dns, Rxi-5sil

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________

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f 40

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____

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ents mainlyn is one of ty low contenFor the enrichod and pa high affinh a suitableds for this ile (CV)-AF

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d Analytical Ch

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th mercury

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audia Hellm

the state over time.on, helps i

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hemistry

018

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________

Collabora

Figure 1:

f 40

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____

ative Project –

: New develop

evelopment

phy (GC) coEI). This tetation allowreement witverlap. Come analytes.

on at atmosh APCI proupled with a

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– Project Partn

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d Analytical Ch

nual Report 2

ical ionizat

Christian Lip

mass spectrouces mass tification viatabases, it i

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018

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pressure

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ossible to dementation, v

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________

Collabora

Figure: D(f – h) gly

f 40

lary zone e

fo

ry zone ele mobility spues for the

glycosylatated with a wral variabilitymensional se

work, the fiMS) has beand 8-aminxity of glycaally, shown

ted in CZE

gnment of p work is a sapplication

____

ative Project –

DT spectra ofycans associ

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or the anal

ectrophoresipectrometryseparation

ion is onewide rangey, the analyeparation is

rst on-line ceen perfomenopyrene-1,an signals wn for both, nE exhibited

peaks in theshowcase fons in the futu

– Project Partn

f AGP (c – e) iated with the

Applied

An

resis coupl

lysis of nat

Julia Klei

is (CZE) bay (IMS) basof complex

of the moe of biologicysis of glycs applied.

coupling of ed to furthe,3,6-trisulfonwas revealenative and A

an unexpe

e DTIMS spor the high pure.

ner: Prof. Chris

and Fetuin e major peaks

d Analytical Ch

nual Report 2

led to drift

tive and AP

n, Sven Me

ased on eleed on mob

x samples.

ost commocal functionscans still re

f CZE with er improve snic acid (Aed which coAPTS-labelected high

pectra conspotential of

stian Neusüß

s separated b

hemistry

018

tube ion m

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ectrophoretilities in the

on post tras and humapresents a

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sidering theif CZE-DTIM

and Dr. Kevin

by CZE.

mobility-ma

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anslational an diseaseschallenging

on mobility capabilitiesed N-glycaresolved b

. Each indivf peaks ob

dimenvationbe exsenceformsrent gas p

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ass spectro

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s way, a chniques an signal the IMS s obser-otentially the pre-isomeric g diffe-

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This is ac

APPI and

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old value of

d Analytical Ch

nual Report 2

ionization m

f photoioniz

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poorly ioniz

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h DIP-APC

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phic separa

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ons and


Annual Report 2018

of 40

Master Theses accomplished 2018

Hayley Sherlee Simpson

Analysis of Cannabis sativa with a four dimensional separation method based on Continuous

Multi-Heart Cutting Gas Chromatography, Ion Mobility and High Resolution Mass Spectrome-

try

Martin Meyer

Development of a SPE protocol for enrichment of phenolic/aromatic compounds

Bachelor Theses accomplished 2018

Tharsiha Kandasamy

Comparison of different Collision Cross Section determination methods using DTIM-qTOF-

MS

Tatiana Kiltau

Optimization of SPE protocols for the enrichment of phenolic / aromatic compounds

Janina Nagel

Characterization of the ingredients of various liquors

Ina Obrock

Origin of Life: High Pressure Simulation and Analysis of Vesicular Peptide Systems

Annika Schubert

Establishment of a CCS database for lipids

Kevin Schulz

Further development of an LC-IM-qTOF-MS/MS method for the determination of lipids in

biological samples and foods


Annual Report 2018

of 40

Accepted and/or Published Scientific Publications 2018

Original Paper / Peer-reviewed

S. Brüggen, O. J. Schmitz A new concept for regulatory water monitoring via high-

performance liquid chromatography coupled to high-resolution mass spectrometry,

accepted in Journal of Analysis and Testing

C. Koch, M. Nachev, J. Klein, D. Koester, O. J. Schmitz, T. Schmidt, B. Sures Degradation

of the polymeric brominated flame retardant "Polymeric FR" by heat and UV, accepted

in Environmental Science & Technology

K. Jooß, S. W. Meckelmann, J. Klein, O. J. Schmitz, C. Neusüß Capillary zone

electrophoresis coupled to drift tube ion mobility-mass spectrometry for the analysis

of native and APTS-labeled N-glycans, accepted in Analytical and Bioanalytical Chemistry

as Paper in Forefront

L. C. Weiss, B. Albada, S. M. Becker, S. W. Meckelmann, J. Klein, M. Meyer, O. J. Schmitz,

U. Sommer, M. Leo, J. Zagermann, N. Metzler-Nolte, R. Tollrian The scent of predation:

Identification of an aquatic infochemical – the Chaoborus kairomone, Nature Chemical

Biology (2018) 14:1133-1139

C. Hellmann, O. J. Schmitz How to deal with mercury in sediments ? A critical review

about used methods for the speciation of mercury in sediments, Chromatographia

(https://doi.org/10.1007/s10337-018-3625-y)

V. Hinnekamp, J. Klein, S. Meckelmann, P. Balsaa, T. Schmidt, O. J. Schmitz Comparison

of CCS Values Determined by Traveling Wave Ion Mobility Mass Spectrometry and

Drift Tube Ion Mobility Mass Spectrometry, Analytical Chemistry (2018) 90:12042-12050

C. Mayer, U. Schreiber, M. J. Dávila, O. J. Schmitz, A. Bronja, M. Meyer, J. Klein, S. W.

Meckelmann Molecular Evolution in a Peptide-Vesicle System, Life (2018) 8: 16 open

access (https://doi.org/10.3390/life8020016)

C. Lipok, J. Hippler, O. J. Schmitz A four dimensional separation method based on conti-

nuous heart-cutting gas chromatography with ion mobility and high resolution mass

spectrometry, Journal of Chromatography A (2018) 1536:50-57

M. Aldrovandi, S. Banthiya, S. Meckelmann, Y. Zhou, D. Heydeck, V.B. O'Donnell, H. Kuhn

Specific oxygenation of plasma membrane phospholipids by Pseudomonas

aeruginosa lipoxygenase induces structural and functional alterations in mammalian

cells, Biochim Biophys Acta Mol Cell Biol Lipids 1863 (2018) 152-164

E. Fahy, J. Alvarez-Jarreta, C.J. Brasher, A. Nguyen, J.I. Hawksworth, P. Rodrigues, S.

Meckelmann, S.M. Allen, V.B. O'Donnell LipidFinder on LIPID MAPS: peak filtering, MS


Annual Report 2018

of 40

searching and statistical analysis for lipidomics, Bioinformatics (2018) 10.1093/

bioinformatics/bty679

Poster Presentations

T. Koehler, J. Klein, O. J. Schmitz, S. W. Meckelmann, Characterization of the human

plasma lipidome using LC-IM-qTOF-MS, Regionalverbandstagung NRW der Lebensmit-

telchemischen Gesellschaft (Essen, Germany) March 2018

L. Montero, K. Rentmeister, S. W. Meckelmann, O. J. Schmitz, S. Buckenmaier, A novel 4D-

analytical platform for Omics Sciences, Agilent Science Fair Posters, March 2018

T. Koehler, O. J. Schmitz, S. W. Meckelmann, Characterization of the human plasma lip-

idome using LC-IM-qTOF-MS, analytica Munich conference (Munich, Germany) April 2018

C. Lipok, J. Klein, F. Uteschil, S. W. Meckelmann, O. J. Schmitz, Determination of complex

natural samples: Marijuana & Cannabinoids with GC+GC-APCI-IM-MS, analytica Munich

conference (Munich, Germany) April 2018

D. Brecht, F. Uteschil, O. J. Schmitz, Investigation of drugs using a novel thermogravim-

etry atmospheric pressure photo ionization mass spectrometry coupling (TG-APPI-

MS), analytica Munich conference (Munich, Germany) April 2018

K. Rentmeister, T. Kriegsmann, J. Klein, S. W. Meckelmann, A. H. Duong, V. H. Pham, O. J.

Schmitz, Analysis of Vietnamese herbs and formulations by means of LC-IM-qTOF-MS,

analytica Munich conference (Munich, Germany) April 2018

C. Lipok, O. J. Schmitz, A generic method for the analysis of complex natural substanc-

es: Marijuana and Cannabinoids with GC+GC-APCI-IMS, 42nd International Symposium

on Capillary Chromatography (Riva, Italy) May 2018 Analytical Method Poster Prize

J. Klein, S. W. Meckelmann, V. Hinnenkamp, T. C Schmidt, O. J. Schmitz, Collision cross

section: Influences and comparability, ASMS 2018 (San Diego, USA) June 2018

S. Stow, R. Kurulugama, G. Stafford, J. Fjeldsted, J. Klein, O. J. Schmitz, T. Causon, S.

Hann, Achieving Highly Accurate CCS Measurements in LC-IM-MS Analyses, ASMS

2018 (San Diego, USA) June 2018

K. Rentmeister, L. Montero, S. W. Meckelmann, S. Buckenmaier, O. J.Schmitz, A novel 4D-

analytical platform for Omics Sciences, 47th HPLC 2018 (Washington, USA) August 2018

J. Li, O. J. Schmitz, Ozone Stress Effect on the Intracellular Metabolites from Cobetia

Marina by Two-dimensional Gas Chromatography with Mass Spectrometer, 9th

analytica Conference (Shanghai, China) October 2018 The Best Poster of the Symposium


Annual Report 2018

of 40

K. Rentmeister, T. Köhler, O. J. Schmitz, S. W. Meckelmann, Characterization of the hu-

man plasma lipidome using LC-IM-qTOF-MS, Lipidomic-Forum (Dortmund, Germany) No-

vember 2018

Invited Lectures / Oral Presentations

Prof. Oliver J. Schmitz

µLC+LC-IM-qTOF-MS for complex samples such as lipidome or proteome

Analytica China conference, Shanghai, China, October 2018

Five-dimensional analysis (LC+LC-IM-qTOF-MS) for complex samples such as

metablome, lipidome or proteome

International Symposium on Metabolic diseases and translational medicine, Dalian, China,

October 2018

LC+LC- and GC+GC-IMS-qTOF-MS as a generic analytical platform and first results

with a new GC-APCI ion source

Tsinghua University, Beijing, China, October 2018

Thousands of separated signals in a four-dimensional separation approach:

How can we manage the data

SECyTA 2018, 18th meeting of the Spanish Society of Chromatography and Related

Techniques, Granada, Spain, October 2018

From one- to five-dimensional analysis platform: pros and cons

2nd 2D-LC / GC Symposium: User meeting for multidimensional chromatography

Frankfurt, Germany, September 2018

Multidimensional Chromatography coupled with Ion-Mobility Mass Spectrometry:

Enough is enough

Analytica conference, Munich, Germany, April 2018

LC+LC- and GC+GC-IMS-qTOF-MS in combination with a CCS data base as a generic

analytical platform

University of Graz, Graz, Austria, March 2018

Einsatz der Ionenmobilitäts-Massenspektrometrie für suspected und non-target

Fragestellungen: Ein kritischer Überblick

IUTA, Duisburg, Germany, March 2018

LC+LC- and GC+GC-IMS-qTOF-MS as a generic analytical platform

Dow-Chemicals, Stade, Germany, January 2018


Annual Report 2018

of 40

Multidimensional Chromatography coupled with Ion Mobility - Mass Spectrometry:

Hype or Ripe?

4th International Ion Mobility Spectrometry (IMS) Meeting, Uetrecht, The Netherlands,

January 2018

Dr. Sven Meckelmann

LC-MS based Lipidomics: From QqQ to IM-qTOF

Analytik Seminar at the University Muenster, Muenster, Germany, July 2018

Characterization of the Human Plasma Lipidome using LC-IM-qTOF-MS

47th International Symposium on High Performance Liquid Phase Separations and Related

Techniques, Washington DC, USA, August 2018

Julia Klein

Collision Cross Section: Comparability of DTIMS and TWIMS and influences of source

parameters

ASMS 2018, San Diego (USA), June 2018

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Annual Report 2018

of 40

Teaching

Chemistry (B.Sc. / M.Sc.)

Lecture Analytical Chemistry I (in German, Prof. Dr. O. J. Schmitz)

Tutorial Analytical Chemistry I (in German, Dr. S. Meckelmann)

Lecture Anaytical Chemistry II (in German, Prof. Dr. O. J. Schmitz)

Tutorial Analytical Chemistry II (in German, Dr. S. Meckelmann)

Water Science (B.Sc. / M.Sc)

Lecture Analytical Chemistry I (in German, Prof. Dr. O. J. Schmitz)

Tutorial Analytical Chemistry I (in German, Dr. S. Meckelmann)

Lecture Anaytical Chemistry II (in German, Prof. Dr. O. J. Schmitz)

Tutorial Analytical Chemistry II (in German, Dr. S. Meckelmann)

Lecture Applied Analytical Chemistry (in English, Prof. Dr. O. J. Schmitz)

Tutorial Applied Analytical Chemistry (in English, Prof. Dr. O. J. Schmitz)

Lecture Environmental Chemistry: Pollutants (in English, Prof. Dr. O. J. Schmitz)

Tutorial Environmental Chemistry: Pollutants (in English, Prof. Dr. O. J. Schmitz)

Exercise Environmental Chemistry: Soil and Waste (in English, Dr. M. Sulkowski)

Environmental Toxicology (M.Sc.)

Lecture Applied Analytical Chemistry (in English, Prof. Dr. O. J. Schmitz)

Tutorial Applied Analytical Chemistry (in English, Prof. Dr. O. J. Schmitz)

Lecture Environmental Chemistry: Pollutants (in English, Prof. Dr. O. J. Schmitz)

Tutorial Environmental Chemistry: Pollutants (in English, Prof. Dr. O. J. Schmitz)

Magisterium

Lecture Environmental Chemistry: Soil (in German, Dr. M. Sulkowski)


Annual Report 2018

of 40

Seminar

Analytical-chemical seminar

(in German/English, Prof. Dr. O. J. Schmitz in cooperation with Prof. Dr. T. Schmidt)

Practical courses

Practical course analytical chemistry

Research practical courses

Teaching and Research Center for Separation

Course 1: Basic Course Liquid Chromatography (in German, Prof. Dr. O. J. Schmitz)

Course 2: Advanced Course Liquid Chromatography (in German, Prof. Dr. O. J. Schmitz)

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applied analytical ch an - uni-due.de · 2018-12-17 · applied ann applied an ana ual universit...

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