-A.N.U. TRANSMISSION ELECTRON MICROSCOPE UNIT

REPORT TO COUNCIL FOR 1988

STAFF OF THE UNIT IN 1988

Head Technical Officer

Senior Technical Officer

Technical Officers

Laboratory Technicians

George Hunter Weston (until August)

Sally Stowe (until March) David James Llewellyn

Katherine Lorna Asmussen Ian Andrew Duff

Diane Davis (from July) Alison Margaret McLean (until June)

CONTENTS

I. FACILITIES AND ADMINISTRATION

2. MAJOR EVENTS IN 1988

3. PUBLICATIONS 3d PUBLICATIONS IN 1988 3.2 CONTRIBUTIONS ACCEPTED FOR PUBLIICA TION

4. MEASURES OF PERFORMANCE 4.1 APPENDIX A SOURCES OF PUBLICATIONS 1983-1988 4.2 APPENDIX B TYPES OF PUBLICATION 1983-1988 4.3 APPENDIX C DISTRIBUTION OF USERS 1983-1988

5. SUMMARY

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1. FACILITIES AND ADMINISTRATION

The Unit serves as a central facility for Transmission Electron Microscopy for RSBS and the Faculties. In addition, use is made of the Unit by other Schools of the Institute of Advanced Studies, by CRES and by CSIRO.

An account of the equipment held by the Unit, and how the facility is administered was given in detail in the Report to Council for 1985.

2. MAJOR EVENTS IN 1988

A bid to the Vice-Chancellor's Major Equipment Fund in 1987, for the purchase of cryoequipment, was successful: a Balzer's Freeze-· Substitution/Low Temperature Embedding Apparatus, and a Polaron Low Temperature Freeze-Dryer with a Sputter Coating attachment were purchased and installed by mid-1988.

Mr George Weston retired at the end of August. He replaced Mr Rod Whitty as the Head Technical Officer in charge of the Unit in 1981. His friendly and helpful approach to Users will be much missed.

A major event of future significance to the provision of Electron Microscope facilities on the ANU Campus is the planned amalgamation of the TEM Unit housed in RSBS and the Scanning Electron Microscope Unit located in the Department of Forestry, the Faculties. The former Deputy Vice-Chancellor, Professor I.G. Ross has been the driving force behind this rationalisation of resources, which will improve the efficiency of both of the existing services.

A new University Electron Microscope Management Committee composed of Professor C. Bryant (Dean, Faculty of Science), Dr P. Cooper (Zoology, the Faculties), Dr R.A. Eggleton (Geology, the Facultieis) and Dr I.G. Morgan (Vision Group, RSBS) has been established from January 1st 1989, to oversee the physical amalgamation of the two Units in RSBS.

3. PUBLICATIONS

3.1 PUBLICATIONS IN 1988

Amarasinghe, V. and Watson, L. (1988). Comparative ultrastructure of microhairs in grasses. Bot. J. Linn. Soc. (Lond 98: 303-319.

Bender, G.L., Goydych, W., Rolfe, B.G. and Nayudu, M. The role of Rhbizobium conserved and host specific nodulation genes in the infection of the non-legume Parasponia andersonii. Mo/. Gen. Genet. 210: 299-306:

Blest, A.O. Turnover of phototransductive membrane in compound eyes and ocelli. Advances in Insect Physiology 20: 1-53.

Blest, A.O. Post-embryonic development of the principal retina of a jumping spider. I. The establishment of receptor tiering by conformational changes. Phil. Trans. R. Soc. Lond. B. 320: 489-504.

- Blest, A.O. and Carter, M. Post-embryonic development of the principal retina of a jumping spider. II. The acquisition and reorganisation of rhabdomeres and growth of the glial matrix. Phil. Trans. R . Soc. Lond. B 320: 505-515.

Blest, A.O., McIntyre, P. and Carter, M. A re-examination of the principal retina of Phidippus johnsonii (Araneae, Salticidae): implications for optical modelling. J. Comp. Physiol. A 162: 47-56.

Busby, C.H. and Gunning, B.E.S. Establishment of plastid-based quadripolarity in spore mother cells of the moss Funaria hygrometri<:a. J. Cell Sci . 91: 127-138.

Busby, C.H. and Gunning, B.E.S. Development of the quadripolar me10t1c cytoskeleton in spore mother cells of the moss Funaria hygrometrica. J . Cell Sci . 91: 139-144.

Chen, H. and Rolfe, B.G. Rhizobium in/ ection of Leucaena leucocephala via the formation of infection threads in curled root hairs. J. Plant Physiol. 132: 3 79-382.

Glikson, M. Miocene algal reef-related deposits in Vanuatu: Possible petroleum source rocks. Greene, H.G. and Wong, F.L. (eds). Circum­Pacific Council for Energy and Mineral Resources Earth Science Series, Houston, Texas 8: 267-274.

Glikson, M. Petroleum source rock study. Miocene rocks of New Ireland, Papua New Guinea. Marlow, M.S., Dadisman, S.Y. and Exon, N.F. (eds). Circum Pacific Council for Energy and Mineral Resources Earth Science Series, Houston, Texas 9: 161-183.

Glikson, M., Simpson, R.W., Taylor, J. and Polach, H. Microscopical TSP studies comparing a city site and suburban sites in Canberra, Australia. Atmosph. Environ. 22: 1745-1758.

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Glikson, M. Transmission Electron Microscopy in the study of oil shales. Proc. lnt. Con/. on oil shales and shale oil. Zhu Yajie (ed). Chem. Ind. Press, Beijing, China: 127-135.

Howitt, S.M., Day, D.A., Scott, K.F. and Gresshoff, P.M. Mutants of Bradyrhizobium ( Parasponia) sp ANU289 affected in assimilatory nitrate reduction also show lowered symbiotic effectiveness. J. Plant Physiol. 132: 5-9.

Huang, S.Z., Djordjevic, M.A. and Rolfe, B.G. Characterization of aberrant infection events induced on Trifolium subterraneum by Rhizobium trifolii Region II mutants. J . Plant Physiol 133: 16-24.

McLennan, I.S. Characterization of a myopathy caused by prostaglancfin dysfunction. Aust. Paediatr . J . Suppl., 21-23.

McLennan, I.S. Characterization of a prostaglandin dysfunction myopathy. Muscle & Nerve 10: 801-810.

Millar, T .J ., Winder, C., Ishimoto, I. and Morgan, I.G. Putative serotonergic bipolar and amacrine cells in the chicken retina. Brain Res. 439: 77-87.

Morgan, I.G. New perspectives on excitotoxicity. Neurosci. Letts. 530: SI 1.

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Morgan, I.G. D-O-Phosphoserine is both a kainic acid and a quisqualic acid antagonist at horizontal, bipolar and amacrine cell receptors. Neurosci . Letts. 530: Sl03.

Morgan, LG., Millar, T.J., Yang, G. and Dvorak, D.R. Retinal circuitry and retinal neurotransmitters. Proc. Aust. Physiol. Pharmacol . Soc . 19: 25-35.

Nicholas, W.L. and Stewart, Metadasynemoides cristatus Nemetology (in press).

A.C. The structure of the cuticle of (Leptolaimina: Ceramonematidae). J.

Prendergast, H.D.V., Hattersley, P.W. and Stone, N.E. New structural/biochemical associations in leaf blades of c4 grasses (Poaceae). Aust. J. Plant Physio/. 14: 403-420.

Spira, A.W., Millar, T.J ., Ishimoto, L., Epstein, M.L., Johnson, C.D. and Morgan, LG. The localisation of choline acetyltransferase-like immunoreactivity in the embryonic chick retina. J. Comp. Neuro/. 260: 526-538.

Warrant, E.J . Pigment migration, optics and image formation in arthropod superposition eyes. In: Proceedings of the IEEE International Conference on Systems, Man and Cybernetics, Beijing, China. Pergamon Press: 1107-1110.

3.2 CONTRIBUTIONS ACCEPTED FOR PUBLICATION

Bender, G.L., Nayudu, M., Le Strange, K.K. and Rolfe, B.G. The modD l gene from Rhizobium strain NGR234 is a key determinant in the extension of host range to the nonlegume Parasponia. Mo/ . Plant. Microbe Inter.

Blest, A.O., The evolution of the visual systems of Salticid spiders: the acquisition of light guide optics in the principal eyes. National Geographic Research Reports.

Blest, A.O. and O'Carroll , D.C. The evolution of the tiered principal retinae of jumping spiders. In: Proceedings, 1st lntrnational Congress on the Neurobiology of Sensory Systems , Goa. Naresh Singh, R. and Strausfeld (eds). Plenum Press, New York.

Cleary, A.L. and Hard ham, A.R. Depolymerization of micro tubule arrays in root tip cells by oryzalin, and their recovery with modified nucleation patterns. Can. J. Bot.

Davis, A.R. Nectaries: secretory structures important to beekeepers. In: Proceedings of the Second Australian and International Bee Congress, Surfers Paradise . J. Rhodes (ed).

Glikson, M., Saxby, J.C. and Lindsay, K. Botryococcus - a planktonic · green alga. In: The Source of Petroleum through the Ages. Transmission Electron Microscopical Studies . Part f . Oil Shales .

- Hardham, A.R. lectin and antibody labelling of surface components of spores of the dieback fungus Phytophthora cinnamomi. Aust. J. Plant Physiol.

Hardham, A.R. Plant Growth and Morphogenesis. In: Plant Physiology.

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Taiz, L. and Zeiger, E. (eds). Benjamin/Cummings Pub. Co., California.

McLennan, I.S. Quantitative relationships between motoneurons and muscle development in X enopus laevis: implications for motoneuron cell death and motor unit formation. J. Comp. Neurol.

McLennan, I.S. Early development and fusion of mucles cells. In: Handbook of Human Growth and Developmental Biology, Chapter 2, Section 18. Timiras, P.S. and Meisami, F. (eds). CRC Press, Florida.

- 4. MEASURES OF PERFORMANCE

4.1 APPENDIX A: SOURCES OF PUBLICATIONS 1983-1987

YEAR RSBS OTHER TOTAL

1983 25 5 30

1984 25 6 31

1985 27 7 34

1986 25 4 29

1987* 28 6 34

1988 21 5 26

TOTALS 151 33 184 (6 years)

4.2 APPENDIX B: TYPES OF PUBLICATION

YEAR PAPERS REVIEWS$ MISC TOTAL

1983 26 4 30

1984 26 3 2 31

1985 32 2 34

1986 29 29

1987* 30 4 34

1988 25 26

TOTALS 168 14 2 184 (6 years)

$ Includes contributions to books, and one book.

• Two 1988 publications were incorrectly attributed to 1987 in the Report to Council for that year.

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- 4.3 APPENDIX C: DISTRIBUTION OF USERS I 983-1987

1983 1984 1985 1986 1987

ALL USERS

TOTAL USERS 81 86 86 90 90

RSBS 64 57 57 59 54

FACULTIES 11 18 20 15 14

IAS (excluding RSBS) 6 8 7 13 17

OTHER 3 2 3 5

ACADEMIC USERS (Including eost-graduate students)

TOTAL ACADEMICS 32 37 34 37 40

RSBS 27 26 24 28 31

FACULTIES 2 5 7 5 3

OTHER 3 6 3 4 6

TECHNICAL OFFICERS AND RESEARCH ASSISTANTS

TOTAL TOs and RAs

RSBS

FACULTIES

OTHER

26

20

3

3

25

16

5

4

27

14

8

5

28

14

4

10

28

11

4

13

1988

78

45

18

13

2

58

35

14

9

20

10

4

6

The TEM unit was established 1n its present form from Nay 1981.

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5. SUMMARY

The pattern of usage and output of publications of the Unit still remain at steady-state after a period of 7 years.

The shift towards multidisciplinary services noted in the Report to Council for 1987 has also been maintained.

A. David Blest Convenor

ANU Transmission Electron Microscope Advisory Committee. Group Leader, Developmental Neurobiology, RSBS