host-pathogen interactions in multidrug-resistant tuberculosiscentro de investigaciones...

Centro de Investigaciones Tecnológicas, Biomédicas y Medioambientales – CITBM www.citbm.pe / citbm@citbm.pe

Host-pathogen interactions inMultidrug-Resistant Tuberculosis

Study of the Molecular Epidemiology, Host Immune responses and Genome Based prediction and Early Identification of MDR-TB in High

Tuberculosis Burden Settings

MDR-TBNet Consortium

Centro de Investigaciones Tecnológicas, Biomédicas y Medioambientales – CITBM www.citbm.pe / citbm@citbm.pe

Oportunity

Problem

Determination

ERANET-LAC

Centro de Investigaciones Tecnológicas, Biomédicas y Medioambientales – CITBM www.citbm.pe / citbm@citbm.pe

Centro de Investigaciones Tecnológicas, Biomédicas y Medioambientales – CITBM www.citbm.pe / citbm@citbm.pe

Centro de Investigaciones Tecnológicas, Biomédicas y Medioambientales – CITBM www.citbm.pe / citbm@citbm.pe

➢ Contributing to the control of a problemthat affects a large part of the worldpopulation, generating a positive impact onthe population.

➢ Establish collaboration, scientific andtechnological exchange.

➢ Research and learning

Centro de Investigaciones Tecnológicas, Biomédicas y Medioambientales – CITBM www.citbm.pe / citbm@citbm.pe

Centro de Investigaciones Tecnológicas, Biomédicas y Medioambientales – CITBM www.citbm.pe / citbm@citbm.pe

Our Research ConsortiumCoordinator

Centro de Investigaciones

Tecnológicas, Biomédicas y Medioambientales, Peru

IRCCS Ospedale San

Raffaele, Italy

Universidade Federal de

Minas Gerais, Brazil

Hospital Sant Joan de Déu,

Spain

Research Center Borstel,

Germany

BIOASTER, France

Centro de Investigaciones Tecnológicas, Biomédicas y Medioambientales – CITBM www.citbm.pe / citbm@citbm.pe

Network strengths

Research Center Borstel (Borstel, Germany)• National Mycobacterium Reference Laboratory• WHO Supranational Reference Laboratory

Hospital Sant Joan de Déu (Barcelona, Spain)• Europeanwide renknown research and

technology facility

IRCCS Ospedale San Raffaele (Milano, Italy)• Research hospital in Molecular Medicine• WHO Collaborating Centre in Tuberculosis

Laboratory Strengthening• WHO Supranational Reference Laboratory for TB

Universidade Federal de Minas Gerais (Belo Horizonte, Brazil)• Top10 university Latin America• Molecular and gentyping reference

Bioaster (Lyon, France)• Technology Research Institute dedicated to

infectious diseases and microbiology in health

CITBM (Callao, Peru)• First peruvian RDI oriented center of excellence• Strong molecular epidemiology background

Centro de Investigaciones Tecnológicas, Biomédicas y Medioambientales – CITBM www.citbm.pe / citbm@citbm.pe

• High prevalence of MDR-TB in Peru and Brazil

• Conventional diagnostic methods are time consuming

• Limit knowledge of immune response to MDR-TB

• MDR-TB host – pathogen interactions not well defined

Introduction

Centro de Investigaciones Tecnológicas, Biomédicas y Medioambientales – CITBM www.citbm.pe / citbm@citbm.pe

Goal

Host-pathogenfactors in MDR-TB

Earlydetection

MDR-TB Prevention

Centro de Investigaciones Tecnológicas, Biomédicas y Medioambientales – CITBM www.citbm.pe / citbm@citbm.pe

Centro de Investigaciones Tecnológicas, Biomédicas y Medioambientales – CITBM www.citbm.pe / citbm@citbm.pe

Cohort study Molecular epidemiology study

Enrolment patients = 24

TB-MDR

(n = 5)

MODS Culture

Baciloscopy

TB Sensible

(n = 10)

Control

(n = 9)

Centro de Investigaciones Tecnológicas, Biomédicas y Medioambientales – CITBM www.citbm.pe / citbm@citbm.pe

• Nurse training in sample collection and patient enrolment at UNIDEC –CITBM and Hospital Dos de Mayo.

• TB molecular typing, sequencing and data analysis. Molecular and Experimental Mycobacteriology Group. Forschungszentrum Borstel -Germany, June 2017.

• Mycobacterial small regulatory RNAs. Emerging Bacterial Pathogens Unit., S. Raffalele Scientific Institute. Milán Italy, May –June 2017.

• Fellow research at CITBM.

Technological transference 2017

Centro de Investigaciones Tecnológicas, Biomédicas y Medioambientales – CITBM www.citbm.pe / citbm@citbm.pe

First steps

Centro de Investigaciones Tecnológicas, Biomédicas y Medioambientales – CITBM www.citbm.pe / citbm@citbm.pe

Our teamCoordinator

Moises Huaman; Leading Scientist (CITBM, Peru)

Paolo Miotto, (IRCCS

Ospedale San Raffaele, Italy)

Daniella Bartholomeu

(Universidade Federal de Minas Gerais, Brazil)

Laia Alsina (Hospital Sant

Joan de Déu, Spain)

Stefan Niemann (Research Center Borstel, Germany)

BIOASTER, France

Coordinator

Moises Huaman; Leading Scientist (CITBM, Peru)

Paolo Miotto, (IRCCS

Ospedale San Raffaele, Italy)

Daniella Bartholomeu

(Universidade Federal de Minas Gerais, Brazil)

Laia Alsina (Hospital Sant

Joan de Déu, Spain)

Stefan Niemann (Research Center Borstel, Germany)

BIOASTER, France

Moisés

Huamán

(CITBM, Perú)

Paolo Miotto

(OSR, Italy)

Coordinator

Moises Huaman; Leading Scientist (CITBM, Peru)

Paolo Miotto, (IRCCS

Ospedale San Raffaele, Italy)

Daniella Bartholomeu

(Universidade Federal de Minas Gerais, Brazil)

Laia Alsina (Hospital Sant

Joan de Déu, Spain)

Stefan Niemann (Research Center Borstel, Germany)

BIOASTER, France

Daniella

Bartolomeu

(UFMG, Brazil)