effect of new cytokinin derivates application on arabidopsis thaliana growth and development

Effect of new cytokinin derivates application on Arabidopsis thaliana growth and

development

Jana KláskováLaboratory of Growth Regulators

Cytokinin metabolism

Cytokinin degradation

N

N N

NH

NH

CH3

CH3

N

N N

NH

NH2

+O CH3

CH3

CKX

Inhibitors of CKX

IIINhibitors of CYtokinin DEgradation

Zatloukal et al., 2008

LGR-2679

LGR-2678

Study of inhibitors in biological assays

0,000

0,050

0,100

0,150

0,200

0,250

0,300

0,350

0,400

0,450

0 20 40 60 80 100

Inhibitor concentration (µM)

AB

S (

57

8 n

m)

INCYDE

INCYDE-FLGR-2679

LGR-2678

CKX assay

Study of inhibitors in biological assays II

4000

5000

6000

7000

8000

9000

10000

11000

12000

0,0

01

uM

0,0

1 u

M

0,1

uM

1 u

M

10

uM

50

uM

Concentration of tested compound [uM]

b-g

ala

cto

sid

as

e a

cti

vit

y

t-zeatin

INCYDE

INCYDE-F

LGR-2679

LGR-2678

Receptor assay (CRE1/AHK4)

10000,00

11000,00

12000,00

13000,00

14000,00

15000,00

16000,00

17000,00

18000,00

19000,00

20000,00

0,0

01

uM

0,0

1 u

M

0,1

uM

1 u

M

10

uM

50

uM

Concentration of tested compound [uM]

b-g

ala

cto

sid

as

e a

cti

vit

y

t-zeatin

INCYDE

INCYDE-F

LGR-2679

LGR-2678

Receptor assay (AHK3)

Study of inhibitors in biological assays III

ARR5:GUS assay

0,1

uM

1u

M

5u

M

10

uM

0,1

uM

1u

M

5u

M

10

uM

0

,1u

M

1u

M

5u

M

10

uM

0,1

uM

1u

M

5u

M

10

uM

0

2

4

6

8

10

12

14

16

18

20

22

24G

US

act

ivity

n

mo

l MU

hm

g p

rote

in[

]-1

-1

INCYDE-F LGR-2679 LGR-2678 BAP

CT

RL

0,1

uM

1u

M

5u

M

10

uM

INCYDE

Effect of INCYDE-(F) application by spraying

CONTROL

10uM INCYDE-F

10uM INCYDE-F

CONTROL 10uM INCYDE

CONTROL 10uM INCYDE-F

10uM INCYDE

Effect of INCYDE-F applicationby spraying

• Low temperature (15°C) for 4 weeks

CONTROL CONTROL CONTROL

1 uM 5 uM 10 uM

Effect of LGR-2679 application by drain

CONTROL

CONTROL

0,1uM X 1x

0,1uM X 3x

0,1uM X 5x

1uM X 1x

1uM X 3x

1uM X 5x

CONTROL

CONTROL

0,1uM X 1x

0,1uM X 3x

0,1uM X 5x

1uM X 1x

1uM X 3x

1uM X 5x

CONTROL LGR 2679; 0,1uM

LGR 2679; 1uMCONTROL

1x 3x 5x

1x 3x 5x

Effect of LGR-2679 application by drain

0,1uM X 3x

0,1uM X 5x

0,1uM X 8x

0,1uM X 3x

0,1uM X 5x

0,1uM X 8x

CONTROL

CONTROL

CONTROL

CONTROL

0,1uM X 3x

0,1uM X 5x

0,1uM X 8x

1uM X 3x

1uM X 5x

1uM X 8x

CONTROL

CONTROL

LGR 2679; 0,1uM

LGR 2679; 1uM

3x 5x 8x

3x 5x 8x

Mgr. Lukáš Spíchal, Ph.D.

Mgr. Ota Blahoušek

Mgr. Markéta Gemrotová, Ph.D

Mgr. Jaroslav Nisler, Ph.D

Thank you for your attention