Effect of new cytokinin derivates application on Arabidopsis thaliana growth and

development

Jana KláskováLaboratory of Growth Regulators

Cytokinin metabolism

Cytokinin degradation

N

N N

NH

NH

CH3

CH3

N

N N

NH

NH2

+O CH3

CH3

CKX

Inhibitors of CKX

IIINhibitors of CYtokinin DEgradation

Zatloukal et al., 2008

LGR-2679

LGR-2678

Study of inhibitors in biological assays

0,000

0,050

0,100

0,150

0,200

0,250

0,300

0,350

0,400

0,450

0 20 40 60 80 100

Inhibitor concentration (µM)

AB

S (

57

8 n

m)

INCYDE

INCYDE-FLGR-2679

LGR-2678

CKX assay

Study of inhibitors in biological assays II

4000

5000

6000

7000

8000

9000

10000

11000

12000

0,0

01

uM

0,0

1 u

M

0,1

uM

1 u

M

10

uM

50

uM

Concentration of tested compound [uM]

b-g

ala

cto

sid

as

e a

cti

vit

y

t-zeatin

INCYDE

INCYDE-F

LGR-2679

LGR-2678

Receptor assay (CRE1/AHK4)

10000,00

11000,00

12000,00

13000,00

14000,00

15000,00

16000,00

17000,00

18000,00

19000,00

20000,00

0,0

01

uM

0,0

1 u

M

0,1

uM

1 u

M

10

uM

50

uM

Concentration of tested compound [uM]

b-g

ala

cto

sid

as

e a

cti

vit

y

t-zeatin

INCYDE

INCYDE-F

LGR-2679

LGR-2678

Receptor assay (AHK3)

Study of inhibitors in biological assays III

ARR5:GUS assay

0,1

uM

1u

M

5u

M

10

uM

0,1

uM

1u

M

5u

M

10

uM

0

,1u

M

1u

M

5u

M

10

uM

0,1

uM

1u

M

5u

M

10

uM

0

2

4

6

8

10

12

14

16

18

20

22

24G

US

act

ivity

n

mo

l MU

hm

g p

rote

in[

]-1

-1

INCYDE-F LGR-2679 LGR-2678 BAP

CT

RL

0,1

uM

1u

M

5u

M

10

uM

INCYDE

Effect of INCYDE-(F) application by spraying

CONTROL

10uM INCYDE-F

10uM INCYDE-F

CONTROL 10uM INCYDE

CONTROL 10uM INCYDE-F

10uM INCYDE

Effect of INCYDE-F applicationby spraying

• Low temperature (15°C) for 4 weeks

CONTROL CONTROL CONTROL

1 uM 5 uM 10 uM

Effect of LGR-2679 application by drain

CONTROL

CONTROL

0,1uM X 1x

0,1uM X 3x

0,1uM X 5x

1uM X 1x

1uM X 3x

1uM X 5x

CONTROL

CONTROL

0,1uM X 1x

0,1uM X 3x

0,1uM X 5x

1uM X 1x

1uM X 3x

1uM X 5x

CONTROL LGR 2679; 0,1uM

LGR 2679; 1uMCONTROL

1x 3x 5x

1x 3x 5x

Effect of LGR-2679 application by drain

0,1uM X 3x

0,1uM X 5x

0,1uM X 8x

0,1uM X 3x

0,1uM X 5x

0,1uM X 8x

CONTROL

CONTROL

CONTROL

CONTROL

0,1uM X 3x

0,1uM X 5x

0,1uM X 8x

1uM X 3x

1uM X 5x

1uM X 8x

CONTROL

CONTROL

LGR 2679; 0,1uM

LGR 2679; 1uM

3x 5x 8x

3x 5x 8x

Mgr. Lukáš Spíchal, Ph.D.

Mgr. Ota Blahoušek

Mgr. Markéta Gemrotová, Ph.D

Mgr. Jaroslav Nisler, Ph.D

Thank you for your attention