control of anthracnose of banana (kluai khai) by the biofumigant fungus muscodor albus strain mfc2

Control of Anthracnose of banana (Kluai Khai ) by the

biofumigant fungus Musco dor albus strain MFC2

Kusuma PotchanaWorraya

PrompangtaChomporn

RaksakiettisakAdviser Miss Aree Sakyim

Purposes• To test the efficiency of MFC2 to inhibit

the anthracnose pathogen in banana. • To find the proper nutrient for MFC2Research Boundary• Testing MFC2 with two pathogen ;

Colletotrichum capsici and Colletotrichum gloeosporioidesระยะเวลาที่�ที่�าการ

ศึ�กษา • Twelve months (November 2006 - November 2007)

Term of Research

Laboratory

• Biotechnological laboratoy of Mahidol University• Biological laboratory of Mahidol Wittayanusorn

School

Method

• Equipment and Substances •Colletotrichum capsici •Colletotrichum gloeosporioides•Muscodor albus strain MFC2•Autoclave• Larminar flow• Pasteur pipettes

ว�ธีดำ�าเนิ�นิการที่ดำลองMethod

• Part 1 Grow MFC2 on PDA

Do hyphal tip with MFC2

Grow MFC2 for 10 -14 days

Method

•Make nutrients for growing MFC2

Nutrient 1 Nutrient 2

Brown rice 15 grams

millet 35 grams

Bran 5 grams water 5 cm2

Method

millet 50 grams

Bran 5 grams water 5 cm2

• Part 2 Grow Pathogens

Method

• Part 3 Test the efficiency of MFC2 to inhibit the anthracnose pathogen in banana on PDA

10 day control

7 day

5 day

Method

• Part 4 Test the efficiency of MFC2 to inhibit the anthracnose pathogen in banana on nutrient 1 and 2

7 day 7 day

ชุ�ดควบค�ม

Method

Results

Results

Table 1 Test on PDA

Plate Diameter (cm)

C.gloeosporioides

C.capsici

1 3.7 1.2

2 3.7 1.6

3 3.6 2.7

Average 3.67 1.83

Control 5.3 7

Pathogens after 5

day

inhibit inhibit

• Table 2 Test on nutrient 1

Plate Diameter (cm)

C.gloeosporioides

C.capsici

PDA

NoPDA

PDA NoPDA

1 2.4 3.9 0.4 1

2 2.8 1 0.4 3

3 1.25

4.2 1 2.5

Average

2.15

3.03 0.6 2.17

Control 4.4 4.4

Pathogen after 5 days

Inhibit Inhibit

Results

• Table 3 Test on nutrient 3 Plate Diameter (cm)

C.gloeospoiroides

C.capsici

PDA

NoPDA

PDA NoPDA

1 3.1 4.3 0.4 0.4

2 2 1.9 3.2 2.7

3 1 3 1.5 3.4

Average

2.03

3.06 1.7 2.17

Control 4.4 4.4

Pathogen after 5 days

Inhibit inhibit

Results

• Muscodor albus Strain 2MFC growed in two different nutrients in order to control two anthracnose pathogens of banana ;Colletotri

chum capsici and Colletotrichum gloeosporioides can inhibit the two pathogens but does not make any difference. Considered with the quantity of the nutrients, MFC2 growed on nutrient mixed with MFC2 has more efficiency to control the pathogens than another which is growed without PDA.

Results

Conclusions and Analysis

การว�เคราะห์�ค�าความแตกต�าง

Conclusions and Analysis

• The nutrient ; water, bran, brown rice and millet, mixed with PDA can grow the MFC2 well and costs low. So this is another way to grow MFC2 to inhibit the two anthracnose pathogens of banana;

Colletotrichum capsici and Colletotrichum gloeosporioides.

Advice

การว�เคราะห์�ค�าความแตกต�าง

• Test with banana, let farmer use and assess.

• Find the proper temperature for growing MFC2.

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Reference

• 200Strobel, G.A., E. Dirkse, J. Sears and C. Markworth. 1 . Volatile antimicrobials from Muscodor albus M MMMMM MMM,

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