almennur fundur um hóttanir móti vistskipanunum á føroysku ... katrin... · føroya...
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![Page 1: Almennur fundur um hóttanir móti vistskipanunum á føroysku ... Katrin... · Føroya Fróðskaparfelag saman við Umhvørvisstovuni – Tórshavn, Færøyene, 9. juni 2015 IRIS](https://reader034.vdocuments.site/reader034/viewer/2022050112/5f4993cdd3977763e60a3dea/html5/thumbnails/1.jpg)
- Er det sant at medisin mot lakselus
tar knekken på rekene?
Almennur fundur um hóttanir móti vistskipanunum á føroysku firðunum Føroya Fróðskaparfelag saman við Umhvørvisstovuni – Tórshavn, Færøyene, 9. juni 2015
IRIS Lab-team: Renée K. Bechmann, Emily Lyng, Stig Westerlund, Shaw
Bamber, Sree Ramanand, Mark Berry, Marianne Nilsen
Elisa Ravagnan - Modelling
NIVA
Katherine Langford – Analysis of DFB Jannicke Moe & Dag Ø. Hjermann - Modelling
Université du Québec à Rimouski Piero Calosi – Scientific advisor
University of Leicester Paul Seear – Genomics
FLUCLIM (Effects of diflubenzuron on Northern shrimps (Pandalus borealis) at ambient and future climate conditions) is funded by Forskningsrådet – Havet og Kysten (2014 – 2016)
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B a c k g ro u n d
Foto: John Øystein Berg, http://www.tronderbladet.no/debatt/article10050639.ece
• Ikke bra for laksens helse • Ikke bra for økonomien til oppdrettsnæringen • Ikke bra for ville laksefisk
Rensefisk
Mekanisk fjerning av lus
Beskyttelsesskjørt
Kjemikalier (= Medisiner)
o Badebehandling Laksen svømmer i et bad med medisin noen minutter (hydrogen-peroksid, azametifos, cypermetrin, deltametrin)
o Spesialfôr Godkjent legemiddel tilsettes fiskefôret (Diflubenzuron, teflubenzuron, emamektin, maks 14 dager)
B e h a n d l i n g a v l a k s e l u s
0
1000
2000
3000
4000
5000
2005 2006 2007 2008 2009 2010 2011 2012 2013 2014
Fôrmiddelet diflubenzuron (kg aktiv substans)
= 8000 tonn spesialfôr med diflubenzuron (Releeze) brukt i 2014
L a k s e l u s er en parasitt som plager laksen
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Shrimps Pandalus borealis
F u t u r e c l i m a t e
Ocean acidification (pH 7.6) and increased temperature (10C)
D i f l u b e n z u r o n from medicated fish feed
Anti-parasitic drug against salmon lice
A m b i e n t
c l i m a t e pH 8.1
7C
Benzoylurea pesticide
Log Kow 3.8
A chitin synthesis inhibitor
- Can salmon lice medicine be a problem for shrimps?
Experiments with shrimps exposed to medicated salmon feed containing the chitin syntesis inhibitor diflubenzuron (DFB) as the active substance
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Feed waste
Medicated fish feed
A d u l t s h r i m p s : • Can eat feed waste and contaminated sediment • Suspended particles of medicated feed and fish feces • Dissolved DFB from feed and fish feces
Positive effect for salmon: Salmon lice die during
moulting
- H o w c a n s h r i m p s b e ex p o s e d ?
L a r v a e : • Suspended particles of
medicated feed and fish feces • Dissolved DFB from feed and
fish feces
14 days treatment of salmon to get rid of
salmon lice
Yes! Free lunch!
Yummi fish feed!
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Stock solutions of sediment slurry with medicated or clean fish feed
10°C 7°C
+ CO2
Continuos flow exposure system
C
Pressure regulator
Solenoid valve
Connection plug
Aqua Medic
system
CO2
Temperature controlled seawater
Heat exchangers
The larvae
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2 g 3.5 mm pellets
Main DFB exposure:
Flow: 200 ml/min
A little extra DFB:
8% fish feed in sediment:
0.5 mg/L
Shrimp larvae were exposed to the medicated fish feed for 2 weeks from hatching
The larvae
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Hatching
Two weeks exposure to medicated pellets with DFB
Climate acclimation of shrimps (females with embryos)
DFB
Ambient Climate
Future Climate
DFB
Age of larvae (days)
10°C, pH 7.6
10°C, pH 7.6
7°C, pH 8.1
7°C, pH 8.1
-14 -7 0 7 14 21 28
Tr e a t m e n t s & T i m e :
DFB at Ambient Climate
DFB at Future Climate
x 6
x 6
x 6
x 6
The larvae
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200
X 6 batches
200
X 6 batches
200
X 6 batches
200
X 6 batches
Ambient climate: 7°C and pH 8.1 Future climate: 10°C and pH 7.6
Control DFB OA/T OA/T + DFB
X 6 X 6 X 6 X 6
Exposure to DFB
Exposure to DFB
The larvae
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Analysis of DFB by Katherine Langford, NIVA
0.0
0.5
1.0
1.5
2.0
2.5
3.0
Total Concentration of DFB in the aquaria (mean + SE)
i.e. DFB attached to feed particles and dissolved in the water
Ambient Climate + DFB Future Climate + DFB
4 h
24 h
4 h
24 h
Controls samples: <0.01 – 0.02 µg/L
µg
/L D
FB
n = 3 n = 3 n = 3 n = 3
DFB was analysed at NIVA by liquid chromatography coupled to tandem mass spectrometry (LC/MS/MS)
Concentrat ion of d i f lubenzuron
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1 mm mesh
200 ml/min After 4 hours: 2 µg/L After 24 hours: 1 µg/L
Conc. of DFB
The pellets after 24 h in the cylinder:
3.5 mm pellets
More DFB remained in the flow-through aquaria than we expected
Analysis of DFB by Katherine Langford, NIVA
The mesh in the cylinder
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SURVIVAL for shr imp lar vae
0
20
40
60
80
100
0 5 10 15 20 25 30
Pe
rce
nt
surv
ival
of
shri
mp
larv
ae
Days post-hatch
- 25 %
- 56 %
- 82 %
E x p o s u r e t o D F B o r c l e a n p e l l e t s
Mean percent survival for 6 replicate batches of shrimp larvae exposed to DFB at two climate scenarios
Ambient Climate Control
Future Climate Control
Ambient Climate + DFB
Future Climate + DFB
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Time
Development time (zoeal progression) for shrimp larvae
Photo: Tandberg & Arnberg
Stage I zoea Stage II zoea Stage III zoea Stage IV zoea
The tail of the shrimp larvae change when they develop from stage II – III - IV
The larvae
Shrimp larvae go through five pelagic zoea stages and one megalopa stage before they settle as post-larva We followed them until stage 4
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0
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0
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% stage II % stage III % stage IV
Day no = age of shrimp larvae Day 6 Day 17 Day 19 Day 9 Day 13
Co
nt
ro
l
DF
B
DFB
Co
nt
ro
l
L A R VA L D E V E L O P M E N T T I M E f o r t h e s u r v i v o r s
Future
Climate
Ambient
Climate D
FB
Co
nt
ro
l
DFB
Co
nt
ro
l
DFB
Co
nt
ro
l
DFB
Co
nt
ro
l
DFB
Co
nt
ro
l
DFB
Co
nt
ro
l
No stage II No stage III No stage IV
Day 27
Exposure to medicated (DFB) or clean (control) pellets
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5 shrimp larvae and
150 Artemia nauplii
in 1 litre seawater.
Wait 5 hours.
Count remaining Artemia.
Feeding rate
for stage II and IV larvae
Pandalus borealis larvae
Artemia nauplii
1 litre
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Nu
mb
er
of A
rtem
ia e
ate
n p
er
shrim
p larv
ae
pe
r ho
ur
0
1
2
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6
1 2 3 4 5 6 7 8
code B
Num
ber
of A
rtem
ia e
aten
per
s
hrim
p la
rvae
per
hou
r Stage II zoea Stage IV zoea
Ambient Climate Ambient Climate Future Climate Future Climate
DFB
DFB
DFB DFB
Control Control
Control
Control
No stage 4, these are stage 3!
Feeding rate for the survivors
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B S T
« B a m b e r ’ s S w i m m i n g T e s t »
L i g h t
I R l i g h t Detector
Shrimp larvae are photo-positive
(they swim towards the light)
- Will DFB affect swimming activity at
ambient or future climate conditions?
Shaw Bamber, IRIS
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Future Climate
• STAGE I: Similar swimming activity for control and DFB exposed larvae.
• STAGE II: Lower swimming acitivity for DFB exposed larvae than control larvae.
STAGE I: Before first moult
STAGE II: Those that survived first moult
Ambient Climate
Similar swimming activity for control and DFB
exposed STAGE I and II larvae.
Example - Raw data
Control (future climate)
DFB (future climate)
Shaw Bamber, IRIS
S w i m m i n g a c t i v i t y
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Day 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34
Day 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28
Determine survival and stage of larvae
2 weeks exposure
2 weeks exposure
1 day exposure
Ambient Climate, 7C
Ambient Climate, 10C
Ambient Climate, 7C
Ambient Climate, 10C
Determine survival
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0
20
40
60
80
100
Day 11 Day 22 Day 33
Ambient Climate Control
Ambient Climate + DFB
Future Climate Control
Future Climate + DFB
7.2C, pH 8.06
9.6 C, pH 7.63
* * *
% s
urv
ival
of
shri
mp
larv
ae
O n e d ay ex p o s u r e : S u r v i va l o f s h r i m p l a r va e
Mean (st.dev.), n = 4 x 140 shrimp larvae
At 10C one day exposure caused high
mortality, but not at 7C
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Effect of DFB exposure on adult shrimps
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Control shrimps moulted successfully in the lab a few weeks after all eggs had hatched
Shrimp exit
Pilot experiment with adult shrimps 2014
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Flubenzurones are chitin synthesis inhibitors: they stop the organism from casting its exuvium during the molting process resulting in death.
Pilot experiment with adult shrimps 2014
A d u l t s h r i m p s d i e d d u r i n g m o u l t i n g
w h e n e x p o s e d t o m e d i c i n e p e l l e t s
c o n t a i n i n g d i f l u b e n z u r o n
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0
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19
- m
ars
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- m
ars
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- m
ars
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- m
ars
27
- m
ars
29
- m
ars
31
- m
ars
2-
apri
l
4-
apri
l
6-
apri
l
8-
apri
l
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- ap
ril
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- ap
ril
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ril
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ril
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ril
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- ap
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mai
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ula
ted
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mb
er o
f m
ou
lts
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Acc
um
ula
ted
nu
mb
er o
f
de
ad s
hri
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s
Moult related mortality
Medicated fish feed (0.6 g Diflubenzuron/kg)
Controls
Successful m o u lt ing
Controls
Medicated fish feed
Control A
Control B
DFB A
DFB B
172 162 179 151
Mixed size shrimps 9.4C
pH 8.0, 400 L tanks
no moulting for previously exposed shrimps
Expo Depuration
Expo Depuration
Number of shrimps at start
Dose: Ca 1 pellet (3.5 mm) per
shrimp per day for 8 days
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Feed waste
There is no such thing as a free
lunch
1-2 weeks exposure (with or without sediment) 1 month depuration Shrimps eat salmon feed (normal or medicine pellets) High mortality at both temperatures and for all sizes of adult shrimps during the 1 month depuration after ending 1-2 week exposure
Effects on adult shrimps
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Rekelarver og andre krepsdyr i planktonet kan få i seg dette…
Rekelarver er veldig følsomme for diflubenzuron fra medisin-fôr!
Feces med flubenzuroner
Fôr - spill
Medisinfôr med flubenzuroner
Lite metaboliseres Alt skilles ut raskt
Flubenzuroner har «lang holdbarhet» i det marine miljø
Voksne reker kan spise dette og få problemer under skallskiftet
Oppsummering!
Strømmen tar med seg rester av medisin-fôr, feces og forurenset
sediment
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Både rekelarver og voksne reker dør under skallskifte når de utsettes for medisin-fôr med diflubenzuron. Resultatene tyder på mer negative effekter ved 10C enn ved 7C
Konklusjon!
Så svaret er:
Ja! Lakselusmedisin kan ta knekken på rekene hvis for mye
slippes ut i fjordene våre.
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Paul Seear is working with the shrimp genomics
R e m a i n i n g p r o j e c t w o r k :
Genomics
The aim is to develop a genetic marker for exposure to chitin synthesis inhibitors in shrimps
Modelling
…. analyse the multiple stressors impacts of increased temperature and OA together with
DFB exposure on Northern shrimp populations
F u t u r e ( 2 0 1 6 ) p r o j e c t w o r k :
Jannicke Moe & Dag Ø. Hjermann
Elisa Ravagnan
Kathy Langford will analyse DFB in the shrimps from the experiments
Chemistry
- How much diflubenzuron have the shrimps accumulated?
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H A V K Y S T
M o r e i n f o :
r k b @ i r i s . n o
Stig Westerlund Emily Lyng
Shaw Bamber Mark Berry
Elisa Ravagnan Marianne Nilsen
Sreerekha S. Ramanand Renée K. Bechmann
Ph
oto
: Elisabeth
Tøn
nessen
Katherine Langford, Jannicke Moe
Dag Ø. Hjermann
Université du Québec à Rimouski
Piero Calosi
University of Leicester
Paul Seear
Partners
T h a n k y o u f o r y o u r a t t e n t i o n !
NFR
The local crowd
Thank you for the money:
Thak you for the science: