acb wakame bioferment advanced - active concepts,...

ACB Wakame Bioferment Advanced

Tomorrow’s Vision… Today!®

Anti-Aging, Antioxidant


*20024 – ACB Wakame Bioferement Advanced

Product Code: 20024

INCI Name: Undaria Pinnatifida Cell Culture Extract

INCI Status: Conforms

Suggested Use Level: 0.5 - 2.0%

Suggested Applications: Anti-Aging, Antioxidant

Anti-Aging: The next step in actives

Live Yeast Cell Derivatives (LYCD) used for wound healing & increasing ATP production

• Based on the works of George Sperti• 1939 – Realized Cell fractions stimulate

metabolic activity & stimulate cellular proliferation

Cell extracts can do more

Stop band-aiding the problem – Find a Solution!

Anti-Aging: The current view

Intrinsic ROS are more of a threat than extrinsic

Mitochondra are like engines that produce ROS as a byproduct of oxidative phosphorylation

Damage builds up overtime

Topical antioxidant address extrinsic ROS• Band-Aid the Solution

Oxidative Phosphorylation

Is a metabolic process that fuels the production of Adenosine Triphosphate (ATP)

- ATP is the major source of energy in our cells

Is an electron transport system- Transfer of electrons from donors to

receptors (oxygen)- Redox reactions are carried out by 5

mitochondrial protein complexes (Electron Transport Chain)

- As electrons pass through the chain, energy is generated that is used to make ATP

Oxidative Phosphorylation is like a bowling ball rolling down stairs –generates energy every time is hits a step

The bowling ball analogy

Mitochondrial Aging

During oxidative phosphorylation, oxygen (final electron receptor) is reduced to water

Leakages occur in the system- Generate hydroxyl & superoxide

radicals instead of water- Cause damage to the mitochondria &

mitochondrial DNA (mtDNA)

As cells divide, damaged mtDNA from old cells pass on to new cells

- Damage to mtDNA accelerates the pace of aging

• The conservation of mtDNA is so strict that genealogists use it to trace lineages

• mtDNA was used to trace human species back to the Mitochondrial eve (140,000 years ago)

mtDNA

Mitochondrial Aging: A vicious cycle

Oxidative damage inhibits

mitochondrial function

Increase in the production of ROS

Mitochondria ceases to generate sufficient levels of

energy

Cellular functions are decreased =

Aging

Mitochondrial aging

• The use of topical antioxidants doesn’t counteract mitochondrial ROS

• The solution is to address the source – our mitochondria

How to break the cycle


Cultured Wakame Cells

- Exposed to UV stress

- Extraction of synergistically active components (Heat Shock Proteins or Heat Shock Elements) – HSP’s

The Solution to Cellular Longevity!


Traditionally live cell derivatives are produced by exposing cells to an environmental stress – UV irradiation

- Our method – supplementing the growth mead used to culture wakame cells

- Allows us to produce a unique cell derivative that is capable of optimizing electron transport to reduce mitochondrial leakage (oxidative phosphorylation)

- This leakage results in the release of damaging free radicals & ROS that ultimately contribute to mitochondrial damage & aging

The Solution to Cellular Longevity!


Stop intrinsic damage by enhancing oxidative phosphorylation

Replenish structural proteins- Cell extracts have been shown to stimulate structural proteins

& wound healing

Increases oxygen Uptake

Powerful Antioxidant

Increases Collagen Synthesis

Target Aging

Efficacy Data: in-vitro

15 Minute Oxygen Consumption Assay

Protocol

• Performed to assess the ability of ACBWakame Bioferment Advanced toincrease cellular oxygen consumption

• Results indicate that ACB WakameBioferment Advanced is capable of inducing changes in oxygen consumption to fibroblasts

• Compared to the untreated controlACB Wakame Bioferment Advancedincreased oxygen consumption by26.8%

Figure 1: Results of the oxygen consumption assay.

0

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120

140

160

Untreated Contorl LYCD ACB Yogurt DermalRespiratory Factor

ACB WakameBioferment Advanced

Oxy

gen

Co

nsu

mp

tio

n (

Pe

rce

nt

of

Bas

elin

e)

Not treatedwith FCCP

Treated withFCCP


DNA MicroArray

Up regulated genes:

a) Structural proteins: Collagen, fibronectin, keratin

b) Oxidative phosphorylation

c) Mitochondrial genes

Down regulated genes:

a) Inflammation

b) ROS

c) NO2,000 genes up-regulated5,000 genes down-regulated


24 Hour Oxygen Consumption Assay

Protocol

• Performed to assess the ability of ACBWakame Bioferment Advanced toincrease cellular oxygen consumptionafter 24 hours of treatment

• Results indicate that ACB WakameBioferment Advanced is capable of inducing changes in oxygen consumption to fibroblasts

• Compared to the untreated controlACB Wakame Bioferment Advancedincreased oxygen consumption by26.8%

0

1

2

3

4

5

6

7

8

9

Treatment

Oxy

gen

Co

nsu

mp

tio

n (

nm

ol p

er 1

0^6

ce

lls)

Untreated Treated with ACB Wakame Bioferment Advanced

Figure 1: Results of the oxygen consumption assay.


ROS Formation Assay

Protocol

• Performed to assess the impact thatACB Wakame Bioferment Advancedhas on intracellular ROS formation

• Results indicate that ACB WakameBioferment Advanced is capable ofreducing ROS formation compared tothe untreated control

Figure 2: ROS Formation Results.

0

0.2

0.4

0.6

0.8

1

1.2

1.4

Untreated 1% ACBWakame

BiofermentAdvanced

0.5% ACBWakame

BiofermentAdvanced

10ug/mLAscorbic

Acid

50ug/mLAscorbic

Acid

100ug/mLAscorbic

Acid

10uM H2O2 150uM H2O2 PBN Pretreat+ H2O2

AA Pretreat+ H2O2

Co

rre

cte

d R

FU

Time 0

Time 1hour


ORAC Assay

Figure 3: Antioxidant capacity of test materials compared to Trolox.

102

9

128

66

0

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100

120

140

100µM Trolox 12.5µM Trolox 0.1% ACB WakameBioferment Advanced

0.05% ACB WakameBioferment Advanced

An

tio

xid

ant

Cap

acit

y (µ

MTE

)

Protocol

• Trolox® was used as the positive control

• Test Quantity: 0.05% & 0.1%

• Fluorescent measurements were taken every two minutes for two hours

• ACB Wakame BiofermentAdvanced showed antioxidant activity at levels as low as 0.05% concentration


NO Formation

Figure 4: NO Formation results shown in relative fluorescent units (RFU).

0

0.01

0.02

0.03

0.04

0.05

0.06

Untreated 1% ACBWakame

BiofermentAdvanced

0.5% ACBWakame

BiofermentAdvanced

0.1% ACBWakame

BiofermentAdvanced

10 ug/mlAscorbic

Acid

50 ug/mlAscorbic

Acid

100 ug/mlAscorbic

Acid

10 uM H2O2

Ch

ange

in R

FU

Time 1hr

Time 6hr

Protocol

• Performed to assess the impact thatACB Wakame Bioferment Advancedhas on intracellular NO (nitric oxide)formation.

• ACB Wakame Bioferment Advancedwas able to reduce NO formation


Procollagen Assay

Figure 5: Comparison of the effects on procollagen levels following treatment.

0

500

1000

1500

2000

2500

3000

3500

1%Magnesium

AscorbylPhosphate

0.5%Magnesium

AscorbylPhosphate

1% ACBWakame

BiofermentAdvanced

0.5% ACBWakame

BiofermentAdvanced

4mM SodiumButyrate

Untreated

Typ

e 1

C-P

ep

tid

e (

ng/

ml)

Protocol

• An ELISA assay was used to assess ACB Wakame Bioferment Advanced impact on collagen synthesis.

• The results indicate that ACB WakameBioferment Advanced is capable ofincreasing the expression of type 1-CPeptide in a fibroblast cell culturemodel.

• Useful in cosmetic formulations intended to increase collagen synthesis


Figure 6: Comparison for percent increase in collagen I synthesis.

Protocol

• An ELISA assay was done to determine if ACB Wakame Bioferment Advanced is capable of inducing collagen I synthesis

• Three concentrations were compared toa positive and negative control.

• The findings indicate that ACB WakameBioferment Advanced is capable of increasing collagen I synthesis in a dose dependent fashion.

0

18

58

108

175

0

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100

120

140

160

180

200

Negative Control Positive Control 1% ACB WakameBiofermentAdvanced

2% ACB WakameBiofermentAdvanced

4% ACB WakameBiofermentAdvanced

Per

cen

t (%

) In

crea

se

Collagen Synthesis


*20024 – ACB Wakame Bioferement Advanced

Product Code: 20024

INCI Name: Undaria Pinnatifida Cell Culture Extract

INCI Status: Conforms

Suggested Use Level: 0.5 - 2.0%

Suggested Applications: Anti-Aging, Antioxidant

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