xiii

 

ABSTRAK

PENGARUH IRAMA SIRKADIAN TERHADAP MOBILISASI

HSC (CD34+) PADA DARAH PERIFER AKIBAT

LATIHAN FISIK SUBMAKSIMAL

Latihan memicu peningkatan produksi ROS yang dapat menyebabkan kerusakan otot rangka. Stres mekanik dan kontraksi otot rangka saat latihan memicu peningkatan GCSF, TNFα, dan IL-6 sebagai respon inflamasi kerusakan otot rangka dan berperan dalam mobilisasi hematopoietic stem cell (HSC). Saat latihan kita harus mengetahui irama sirkadian agar dapat menghasilkan manfaat yang berguna bagi tubuh. Diketahui bahwa ROS setelah latihan fisik submaksimal sesi sore hari lebih tinggi dibandingkan dengan setelah latihan fisik submaksimal sesi pagi hari. Salah satu faktor lain yang mempengaruhi mobilisasi HSC (CD34+) adalah peningkatan produksi ROS. Tujuan penelitian adalah untuk membuktikan perbedaan perubahan persentase HSC pada darah perifer akibat latihan fisik submaksimal sesi pagi hari dan sesi sore hari. Penelitian ini merupakan penelitian eksperimental laboratoris pretest-posttest one-group design. Subjek penelitian merupakan mahasiswa laki-laki Fakultas Ilmu Keolahragaan, Universitas Negeri Surabaya. Pengambilan sampel menggunakan metode random sampling. Subjek penelitian dalam kondisi sehat, usia 21-25 th, BMI Normal (tinggi badan 165-185 cm). Latihan fisik submaksimal dilakukan pada sesi pagi hari dan sesi sore hari. Pengambilan sampel darah dilakukan pretest dan posttest latihan fisik submaksimal baik sesi pagi hari maupun sesi sore hari untuk mengukur persentase HSC (%CD34+) menggunakan teknik flowcytometri. Hasil penelitian menunjukkan peningkatan persentase HSC (%CD34+) pasca latihan fisik submaksimal sesi pagi hari dan penurunan pada persentase HSC (%CD34+) pasca latihan fisik submaksimal sesi sore hari.

Kata kunci: hematopoietic stem cell, CD34+, latihan fisik submaksimal

ADLN - PERPUSTAKAAN UNIVERSITAS AIRLANGGA

TESIS PENGARUH IRAMA SIRKADIAN... DHONI AKBAR GHOZALI

xiv

 

ABSTRACT

CIRCADIAN RHYTHM EFFECT ON HSC (CD34 +) MOBILITAZION

IN PERIPHERAL BLOOD AS A RESULT OF

SUBMAXIMAL PHYSICAL EXERCISE

Exercise triggers increased production of ROS caused skeletal muscle damage. Mechanical stress and contraction of skeletal muscle during exercise leads to increased GCSF, TNFα and IL-6 as an inflammatory response of skeletal muscle damage and plays a role in the mobilization of hematopoietic stem cell (HSC). At practice we should be aware of circadian rhythm in order to produce useful benefits for the body. It is known that physical exercise submaximal ROS after the afternoon session was higher than after submaximal physical exercise in the morning session. One of the other factors that influence the mobilization of HSC (CD34+) is increased production of ROS. The research objective was to prove the difference in the percentage change in HSC in peripheral blood as a result of submaximal exercise in the morning session and an afternoon session. This research was a laboratory experimental pretest-posttest one-group design. The research subject is a male student of the Faculty of Sport Science, State University of Surabaya. Sampling used random sampling method. The subject of research are in a healthy condition, age 21-25 years old, Normal BMI (height 165-185 cm). Submaximal physical exercise performed in the morning session and an afternoon session. Blood sampling performed pretest and posttest submaximal exercise either the morning session or the afternoon session to measure the percentage of HSC (%CD34+) using techniques flowcytometri. The results showed an increase in the percentage of HSC (%CD34+) after submaximal exercise in the morning session and a decrease in the percentage of HSC (%CD34+) submaximal exercise after the afternoon session.

Keywords: hematopoietic stem cell, CD34+, submaximal physical exercise

 

ADLN - PERPUSTAKAAN UNIVERSITAS AIRLANGGA

TESIS PENGARUH IRAMA SIRKADIAN... DHONI AKBAR GHOZALI