Presentation

Montreal, Canada

INRIA/LIX team AMIB Campus de l'Ecole Polytechnique;

Institute of Problems of Biology and Biotechnology, al-Farabi Kazakh National University;

National Nanotechnology Laboratory of Open Type, al-Farabi Kazakh National University;

Laboratory of Molecular Immunology and Immunobiotechnology,

M.A. Ajtkhozhin Institute of Molecular Biology and Biochemistry

Olga Berillo

PhD, Bioinformatics, Biotechnologist, Scientific Researcheromalaheenee@gmail.com

https://github.com/omalaheenee/mirpy

ca.linkedin.com/in/olgaberillo

Figure 1. Statistics data of in-miRNAs

participated in oncogenesis

Количество статей в PubMedArticles in PubMed NCBI database

Research methods

Computer programs

MiRNAFinder: search for intronic pre-miRNAs in human genome

RNAHybrid, ERNAHybrid, miRTarget: search for miRNA binding sites in mRNAs

GeneNet Builder: visual presentation of gene networkUNAFold: construction of 2D-structures

Developed programs

miRAFinder и GeneAFinder: to get an information about miRNAs and genes TmiRSO и TranslmiRNA: to get a perfect complementary miRNA binding sites and oligopeptides coded by them TmiRUSite и TmiROSite: to get a nucleotide fragments of mRNAs with binding sites and amino acid sequences coded by them.

Objects of researchesNucleotide sequences of 2500 miRNAs and 17500 human genes.

Majority of them participate in development of cancer

Figure 2. Gene net between

host genes and target

genes participated

in esophageal

cancer

Note: Internal circle:

host genes; external circle: target genes

SCP2SCP2miRmiR-1273-1273gg-3-3ppmiRmiR-1273-1273gg--5p5pmiR-1273fmiR-1273fmiR-5095miR-5095

Figure 3: Variability of nucleotides (1) in miR-548j binding sites of orthologous PTPN12 genes and variability of amino

acids (2) in orthologous PTPN12 proteins

1

2

Table 1 – Features of microRNA binding sites in the same places of mRNA

MLLT6 mRNA, 1043 н., CDS

mRNA MLLT6 5' C G G G 3' UGCAGUCUC UCCCU CA ACGUCAGAG AGGGA GU miR-4419a 3' G 5'

mRNA MLLT6 5' C G C 3' UGCAGUCUC UCCCUG AUGUUAGAG AGGGAC miR-4441 3' G A 5'

TLE3 mRNA, 4664 н., 3'UTR

mRNA TLE3 5' A A 3' UUGGGGGGUGGG GAGGGGG GACCCCCCAUCU CUUCUCU miR-6515-3p 3' A 5'

mRNA TLE3 5' U C 3' UUGGGGGG GGGAGAGGGGGA GACCCUCC CCCUCUUCUCCU miR-877-3p 3' U 5'

Figure 4. 3'UTRs with miR-1273 family binding sites

Notes: points are the same nucleotedes, results received by miRTarget and TmiRUSite programs

Figure 5. 3'UTRs with miR-619-5p group binding sites (1 part)

Notes: points are the same nucleotedes, results received by miRTarget and TmiRUSite programs

Figure 6. 3'UTRs with miR-619-5p group binding sites (2 part)

Notes: points are the same nucleotedes, results received by miRTarget and TmiRUSite programs

Figure 7. microRNA binding sites in IKZF3 mRNA at 2D-

structure (fragments of 3’UTR)

Figure 8: conserved

microRNA binding sites in normal and optimised

mRNA sequences of Parvovirus B19

isolates

Note: Yellow color:

binding sites; Red color:

nucleotides of normal mRNA differed from

optimized mRNA;*: conserved mRNAs

of 64 isolates

Figure 9. Definition method of differentiation pathway in the CFC test (morphology)

Thank you for attention

omalaheenee@gmail.comhttps://github.com/omalaheenee/mirpy

ca.linkedin.com/in/olgaberillo