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June 20 – 22Haeundae Grand Hotel, Busan, Korea

2019International Symposiumand Annual Meetingof the KSABC

Hosted by

Co-organized by

Sponsored by


This work was supported by the Korean Federation of Science and

Technology Societies (KOFST) Grant funded by the Korean Government.


Contents

004 Floor Plan

005 Timetable

006 Program Schedule (Poster Presentation)

083 Plenary Lecture

085 Special Lecture

087 Award Lectures

091 International Symposia

109 Symposia

137 Graduate Student Presentation

171 Bio-exhibition

177 Author Index

Page

4

2019 International Symposium and Annual Meeting of the KSABCFloor Plan

Grand Ballroom

Opening Ceremony, Plenary Lecture, Special Lecture, IS, Award Lectures, Welcome Reception, General Assembly Meeting & Closing Ceremony

Hall A IS1, S1, S6, GS1

Hall B IS2, S2, S7, GS1

Hall C IS3, S3, S8, GS2

Studio G IS4, S4, GS3

Studio 3 IS5, S5, GS4

Lobby Bio-exhibition

Convention Hall Poster Presentation, Bio-exhibition

2F

Poster Presentation

Bio-exhibition

Convention Hall

Hall (Grand Ballroom)

Studio1

Studio2

Studio3

Studio G

Connection PassageRegistration

Bio-exhibition

C B A

PL Plenary LectureSL Special LectureAL Award Lectures

ISInternational

Symposia

IS1 BiochemistryㆍMolecular BiologyIS2 Environmental SciencesIS3 Natural ProductsㆍBioactive MaterialsㆍBiomedical SciencesIS4 Food SciencesIS5 Applied Microbiology

S Symposia

S1 BiochemistryㆍMolecular BiologyS2 Environmental SciencesS3 Natural ProductsㆍBioactive MaterialsㆍBiomedical SciencesS4 Food SciencesS5 Applied MicrobiologyS6 Agro-Bio Genome EditingS7 Forensic ScienceS8 Pesticide Positive List System

GS

Graduate

Student

Presentation

GS1 BiochemistryㆍMolecular BiologyGS2 Environmental SciencesGS3 Natural ProductsㆍBioactive MaterialsㆍBiomedical SciencesGS4 Food Sciences

PPoster

Presentation

PBM BiochemistryㆍMolecular BiologyPNB Natural ProductsㆍBioactive MaterialsㆍBiomedical SciencesPES Environmental SciencesPFS Food SciencesPAM Applied Microbiology

B Bio-exhibition

5

June 20 – 22 l Haeundae Grand Hotel, Busan, KoreaTimetable

09:00- Field Trip

June 20 Thu.

June 21 Fri.

June 22 Sat.

Venue Hall A Hall B Hall C Studio G Studio 3 Lobby & Convention Hall

09:00- Registration

Bio-exhibition

09:30-11:00 GS1 GS2 GS3 GS4

11:10-11:30 Opening Ceremony (Grand Ballroom)

11:30-13:00 Lunch

13:00-13:40 PL-1

13:40-14:10 IS1-1

14:10-14:40 IS2-1

14:40-14:50 Break

14:50-15:20 IS3-1

15:20-15:50 IS4-1

15:50-16:20 IS5-1

16:20-16:30 Break

16:30-17:00 SL-1

17:00-18:30 Poster Presentation I (Convention Hall)

18:30-20:00 Welcome Reception (Grand Ballroom)

Time

Venue

Hall A Hall B Hall C Studio G Studio 3 Lobby & Convention Hall

09:00- Registration

Bio-exhibition

09:30-10:30 IS1 IS2 IS3 IS4

IS510:30-10:40 Break

10:40-12:10 S1 S2 S3 S4

12:10-13:30 Lunch

13:30-15:00 S6 S7 S8 - S5

15:00-16:00 Poster Presentation II (Convention Hall)

16:00-16:40 Award Lectures (Grand Ballroom)

16:40-17:10General Assembly Meeting &

Closing Ceremony (Grand Ballroom)

Time

Grand Ballroom

Opening Ceremony, Plenary Lecture, Special Lecture, IS, Award Lectures, Welcome Reception, General Assembly Meeting & Closing Ceremony

Hall A IS1, S1, S6, GS1

Hall B IS2, S2, S7, GS1

Hall C IS3, S3, S8, GS2

Studio G IS4, S4, GS3

Studio 3 IS5, S5, GS4

Lobby Bio-exhibition

Convention Hall Poster Presentation, Bio-exhibition

6

2019 International Symposium and Annual Meeting of the KSABC

Graduate Student Presentation

Program Schedule

GS1-1

GS1-2

GS1-3

GS1-4

09:30-09:40

Role of Cellular NAG-1/GDF15 in Tumorigenesis

Jaehak Lee, Ilju Kim, Seung Joon Baek*

Laboratory of Signal Transduction, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of Korea

09:40-09:50

Biogenesis of outer membrane vesicles derived from Escherichia coli small RNAs and their implications

Sohee Lee, Kwang-sun Kim*

Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University, Busan 46241, Republic of Korea

09:50-10:00

Antioxidant Components and Activities of FRCE on H2O2-induced Oxidative Stress in HepG2 Cells

Sang Hyuk Lee, Ryong Kong, Jae Won Lee, Doo Jin Choi, Dae Young Lee, Geum-Soog Kim,

Young-Seob Lee*

Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration (RDA), 92 Bisan-ro, Eumsung, Chungbuk 27709, Republic of Korea

10:00-10:10

Alternative splicing of thrombospondin gene in tumorigenesis

Yu Kyung Hong1, Chang Hwan Ryu2, Jun Sun Ryu2, Seung Joon Baek1*1Laboratory of Signal Transduction, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of Korea, 2Department of Otolaryngology-Head and Neck Surgery, Center for Thyroid Cancer, Research Institute and Hospital, National Cancer Center, Goyang, Republic of Korea

June 20 (Thu), Hall A+B

Chair: Chanseok Shin (Seoul National University)

GS1 Biochemistry · Molecular Biology

7

June 20 – 22 l Haeundae Grand Hotel, Busan, Korea

10:10-10:20

Morin hydrate inhibited dipeptidyl peptidase 1 and increased autophagic flux in response to ESBL-E.coli in HepG2 cell line model of sepsis

Chanchal Sharma, Sun Chul Kang*

Department of Biotechnology, Daegu University, Gyeongsan, Gyeongbuk 38453, Republic of Korea

10:20-10:30

Overexpression of ginseng-derived PgpPLAIIIβ altered cell growth patterns and reduced lignified fiber cells in Arabidopsis and hybrid poplars

Jin Hoon Jang, Ok Ran Lee*

Department of Applied Plant Science, College of Agriculture and Life Science, Chonnam National University, Gwangju 61186, Republic of Korea

10:30-10:40

Enhancement of resveratrol production in peanut sprouts by elicitation

Soyoung Kim1,2, Yu Jeong Jeong1, Suhyun Park1, Jae Cheol Jeong1, Bo-Keun Ha2, Cha Young Kim1*1Biological Resource Center, Korea Research Institute of Bioscience Biotechnology (KRIBB), Jeongeup 56212, Republic of Korea, 2Department of Plant Biotechnology, College of Agriculture & Life Sciences, Chonnam National University, Gwangju 61186, Republic of Korea

10:40-10:50

Integrated analyses of shotgun proteomics and metabolomics reveal its association with rise of secondary metabolites of warm water imbibition soybean seeds

Cheol Woo Min1, Hyejin Hyeon2, Ye Eun Cheon1, Ravi Gupta1, Gi Hyun Lee1, Byong Won Lee3,

Hyung Won Ryu4, Jae Kwang Kim2, Sun Tae Kim1*1Department of Plant Bioscience, Life and industry Convergence Research Institute, Pusan National University, Miryang 50463, Republic of Korea, 2Division of Life Sciences, Incheon National University, Incheon 22012, Republic of Korea, 3Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration, Suwon 16429, Republic of Korea, 4Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejun 28116, Republic of Korea

10:50-11:00

MicroRNA Expression Profiling of Female Developmental Stages of Asian Honeybees (Apis cerana)

Igojo Kang, Chanseok Shin*

Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea

GS1-5

GS1-6

GS1-7

GS1-8

GS1-9

8


09:30-09:40

Dissipation Pattern of Insecticide Sulfoxaflor Applied on Minor Crops

Aniruddha Sarker, Sang-Hyeob Lee, Se-Yeon Kwak, Hyo-Jeong Kim, Hye-Rin Jeong, Ae-ji Nam,

Jang-Eok Kim*

School of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea

09:40-09:50

Development and Validation of Multi-residue Screening Method for 439 Pesticides in Vegetable Juice Using LC-MS/MS and GC-MS/MS

Eunyoung Park1, Yongho Shin1, Junghak Lee1, Jiho Lee1, Mai Rehan1, Jae Chul Cheong2,

Hye Suk Lee3, Jeong-Han Kim1*1Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea, 2Forensic Chemistry Laboratory, Forensic Genetic & Chemistry Division, Supreme Prosecutors’ Office, Seoul 06590, Republic of Korea, 3College of Pharmacy, The Catholic University of Korea, Gyeonggi-do 14662, Republic of Korea

09:50-10:00

Control of root-knot nematodes by a mixture of maleic acid and copper sulfate

Jehyeong Yeon1, Ae Ran Park1, Nan Hee Yu1, Cuong Viet Han1, Ho Myeong Kim2,

Hae Woong Park2, Jin-Cheol Kim1*1Department of Agricultural Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Science, Chonnam National University, Gwangju 61186, Republic of Korea, 2R&D Division, World Institute of Kimchi, Gwangju 61755, Republic of Korea

10:00-10:10

Development of Analytical Methods for Chinomethionate in Livestock Products

Seung-Hyun Yang, Hoon Choi*

Department of Bio-Environmental Chemistry, College of Agriculture and Food Sciences, Wonkwang University, Iksan 54538, Republic of Korea

June 20 (Thu), Hall C

Chair: Won-Chan Kim (Kungpook National University)

GS2 Environmental Sciences

GS2-1

GS2-2

GS2-3

GS2-4

9


GS2-5

GS2-6

GS3-1

GS3-2

10:10-10:20

Spatial distributions of persistent organic pollutants (POPs) and metals in paddy soil from major industrial areas in Korea

Min-Kyu Park1, Jin-Woo Jeon1, In-Gyu Cho1, Sung-Eun Lee2, Sung-Deuk Choi1*1School of Urban and Environmental Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Republic of Korea, 2School of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea

10:20-10:30

Establishment of Soil Management Guideline for Spinach Cultivation in Soils Contaminated with Endosulfan

Se-Yeon Kwak, Sang-Hyeob Lee, Aniruddha Sarker, Hyo-Jeong Kim, Hye-Rin Jeong, Ae-Ji Nam,

Jang-Eok Kim*


09:30-09:40

Synthesis and evaluation of antibacterial activity of heterocyclic compounds against Staphylococcus species

Jong Hoon Shin, Kwang-sun Kim*


09:40-09:50

LC-MS-Guided Isolation of Anti-inflammatory Compounds from Roots of Pistacia weinmannifoliaSeong Mi Lee1,2, Yang Hee Jo1, Hyung Won Ryu1, Su Ui Lee1, Eun Sol Oh1, Sang Woo Lee3,

Sang Ho Choi3, Wan-Yi Li4, Bang Yeon Hwang2, Sei-Ryang Oh1*1Natural Medicine Research Center, KRIBB, 30-Yeongudanji-ro, Ochang-eup, Cheongwon-gu, Cheongju-si, Chungbuk 28116, Republic of Korea, 2College of Pharmacy Chungbuk National University, 194-21, Osongsaengmyeong 1-ro, Osong-eup, Heungdeok-gu, Cheongju-si, Chungbuk 28160, Republic of Korea, 3International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea, 4Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China

June 20 (Thu), Studio G

Chair: Joo-Won Nam (Yeungnam University)

GS3 Natural Products · Bioactive Materials · Biomedical Sciences

10


09:50-10:00

Comparative toxicity of polyhexamethylene guanidine phosphate from rats (Sprague-Dawley: Wistar: Fischer 344)

In-Hyeon Kim1,2, Sung-Hwan Kim1,2, Doin Jeon1, Hyeon-Young Kim1,3, Kicheon Kim1,

Kyuhong Lee1,2*1National Center for Efficacy Evaluation of Respiratory Disease Product, Korea Institute of Toxicology, Jeollabuk-do 56212, Republic of Korea, 2Department of Human and Environmental Toxicology, University of Science & Technology, Daejeon 34113, Republic of Korea, 3College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Republic of Korea

10:00-10:10

Biophotonic Effect of Hypericin in Caenorhabditis elegansTram-Anh Ngoc Le, Kyungsu Kang*

Natural Product Informatics Research Center, KIST Gangneung Institute of Natural Products, Gangneung,Gangwon-do 25451, Republic of Korea

10:10-10:20

Iridoids from the Syringa dilatata flowers and their anti-inflammatory activity

Yeong-Geun Lee1, Jung Eun Gwag1, Hyoung-Geun Kim1, Youn-Hyung Lee2, Dong Sung Lee3,

Nam-In Baek1*1Graduate School of Biotechnology and Department of Oriental Medicine Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea, 2Department of Horticultural Biotechnology of College of Life Sciences, Kyung Hee University, Yongin 17104, Republic of Korea, 3College of Pharmacy, Chosun University, Gwangju 61452, Republic of Korea

10:20-10:30

Mushroom derived β-glucan ameliorates high fat diet induced metabolic syndrome in mice

Karthika Muthuramalingam1, Chang Min Choi1, Dharanibalan Kasiviswanathan1, Young Mee Kim1,

Moonjae Cho1,2*1Department of Biochemistry, School of Medicine, Jeju National University, Jeju 63243, Republic of Korea, 2Department of Biochemistry, School of Medicine, Institute of Medical Science, Jeju National University, Jeju 63241, Republic of Korea

GS3-3

GS3-4

GS3-5

GS3-6

11


10:30-10:40

Synergistic combination of chitosan and aminoglycoside antibiotics against Mycobacterium tuberculosisHan-woo Kim1, In-dal Park1, Chul-ho Ok2, Won-Kyo Jung3, Ji-yeun Kate Kim1*1Department of Microbiology, Kosin University College of Medicine, Busan, Republic of Korea, 2Department of Internal Medicine, Kosin University College of Medicine, Busan, Republic of Korea, 3Department of Biomedical Engineering, Pukyong National University, Busan, Republic of Korea

10:40-10:50

Acute Toxicity Assessment of Polyhexamethylene Guanidine Phosphate Following Intravenous Injection and Intratracheal Instillation in Rats

Hyeon-Young Kim1,2, Sung-Hwan Kim1,3, Doin Jeon1, In-Hyeon Kim1,3, Kicheon Kim1, Kyuhong Lee1,3*1National Center for Efficacy Evaluation of Respiratory Disease Product, Korea Institute of Toxicology, Jeollabuk-do 56212, Republic of Korea, 2College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Republic of Korea, 3Department of Human and Environmental Toxicology, University of Science & Technology, Daejeon 34113, Republic of Korea

10:50-11:00

Immunomodulatory activity of Weissella confusa DD_A7 in zebrafish larvae

Debasish Kumar Dey, Sun Chul Kang*


09:30-09:40

Development of improved analytical methods for wheat glutenin proteins

You-Ran Jang1,2, Jong-Yeol Lee1*1National Institute of Agricultural Science, RDA, Jeonju 54874, Republic of Korea, 2Department of Food Science & Technology, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju 54896, Republic of Korea

09:40-09:50

Multiplex PCR assay for genetically modified cotton

Jin-Young Choi, Ji-Eun Park, Hyun-Joong Kim, Hae-Yeong Kim*

Department of Food Science and Biotechnology, Graduate School of Biotechnology, Kyung Hee University,Republic of Korea

GS3-7

GS3-8

GS3-9

GS4-1

June 20 (Thu), Studio 3

Chair: Hoon Choi (Wonkwang University)

GS4 Food Sciences

GS4-2

12


09:50-10:00

Effect of Different Processing Methods on Quality and Functional Ingredients in Asparagus Juice Production

Thu Thi Nguyen, Chi Rac Hong, Sang Min Kim*

Smart Farm Research Center, KIST Gangneung Institute of Natural Products, Gangneung, Gangwon-do 25451, Republic of Korea

10:00-10:10

RDA Database 2.0: Phenolic Acids from Agro-food Resources Based on the Mass Spectrometry

Min-Ki Lee1, Heon-Woong Kim1, Seon-Hye Lee1, Gelila Asamenew1, Suji Lee1, Hyun-Ah Jung2,

Sang-Hoon Lee1, Seon Mi Yoo1, Jung-Bong Kim1*1National Academy of Agricultural Science, Rural Development Administration, 166, Nongsaengmyeong-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do, Republic of Korea, 2Department of Food Science and Human Nutrition, Chonbuk National University, 567, Baekje-daero, Deokjin-gu, Jeonju-si, Jeollabuk-do, Republic of Korea

10:10-10:20

PathVisio as a tool for visualizing metabolic network dynamics using metabolic profiling of pepper fruits

Tae Jin Kim1, Hyejin Hyeon1, Tae Gyu Yi2, Nam Il Park2, Jae Kwang Kim1*1Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Republic of Korea, 2Department of Plant Science, Gangneung-Wonju National University, 7 Jukheon-gil, Gangneung 25457, Republic of Korea

10:20-10:30

Analytical Method Development and Analysis of Azoxystrobin Residue in Sorghum Using LC-MS/MS

Xiu Yuan, Zhehao Jin, Jeonghak Lee, Ahreum Lee, Eunyoung Park, Rakdo Ko, Mai Rehan,

Seohyun Kim, Boeun Ju, Jeong-Han Kim*

Department of Agricultural Biotechnology, Seoul National University, Republic of Korea

10:30-10:40

Metabolite Profiling and Antioxidant Activity of New Early to Mid-season Apple Cultivars for a Warmer Climate and Traditional Cultivars

Inhwan Kim, Jihyun Lee*

Department of Food Science and Technology, Chung-Ang University, Anseong 72-1, Republic of Korea

GS4-3

GS4-5

GS4-4

GS4-6

GS4-7

13


10:40-10:50

Characterization of Phenolic Compounds in the Fruits of Prunus Genus Using UPLC-DAD-QToF/MS

Suji Lee1,2, Heon-Woong Kim1, Min-Ki Lee1, Seon-Hye Lee1, Gelila Asamenew1, Yong-suk Kim2,

Jung-Bong Kim1*1Department of Agro-Food Resources, National Academy of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea, 2Department of Food Science and Technology, Chonbuk National University, Jeonju 54896, Republic of Korea

10:50-11:00

Oral administration of ethanol extract ameliorates atopic dermatitis in house dust mite/DNCB-stimulated NC/Nga mice model

Eui Jeong Han1, Eun Ji Shin2, Min Ju Kim1,2, Disanayaka Mudiyanselage Dinesh Madusanka1,

Mawalle Kankanamge Hasitha Madhawa Dias1, Hyun-Soo Kim3, Seo-Young Kim3, You-Jin Jeon3,

Youngheun Jee4, Ginnae Ahn1,5*1Department of Food Technology and Nutrition, Chonnam National University, Yeosu, Republic of Korea, 2Nantapizza 5000, Damyang, Republic of Korea, 3Department of Marine Life Science, Jeju National University, Jeju, Republic of Korea, 4Department of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Jeju, Republic of Korea, 5Department of Marine Bio-Food Sciences, Chonnam National University, Yeosu, Republic of Korea

13:00-13:40

Determination of toxicodynamic differences of sodium channel isoforms to pyrethroids in juvenile and adult rat brain tissue microtransplanted into Xenopus oocytes

John M. Clark*

Department of Veterinary & Animal Sciences, University of Massachusetts, Amherst, MA 01003-9301, USA

GS4-8

GS4-9

PL-1

Plenary Lecture

June 20 (Thu), Grand Ballroom

Chair: Ki Hun Park (Gyeongsang National University)

14


16:30-17:00

Policy of Research Grant Support by Division of Life Sciences NRF

Young Yang*

Division of Life Sciences, National Research Foundation of Korea, Republic of Korea

13:40-14:10

Improving end-use quality and immunogenic potential of wheat flour

Susan B. Altenbach*

USDA-ARS Western Regional Research Center, Albany, CA, USA

09:30-10:00

Direct role of MUTE in orchestrating stomatal differentiation

Soon-Ki Han1*, Keiko U. Torii1,2,3

1Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Chikusa, Nagoya 464-8601, Japan, 2Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195-1800, USA, 3Department of Biology, University of Washington, Seattle, WA 98195, USA

10:00-10:30

In vivo Imaging of Reactive Oxygen Species (ROS) Using a New Ratiometric ROS Bio-reporter for Studying the Function of ROS

Su-Hwa Kim, Won-Gyu Choi*

Department of Biochemistry and Molecular Biology, University of Nevada, Reno, NV 89557, USA

SL-1

IS1-1

IS1-3

IS1-2

Special Lecture


Chair: Hyung-Joo Kwon (Hallym University)

June 21 (Fri), Hall A

Chair: Yeon Jong Koo (Chonnam National University)

International Symposia


Chair: Young-Soo Keum (Konkuk University)

IS1 Biochemistry · Molecular Biology

15


IS2-1

IS2-2

IS2-3

14:10-14:40

Target Identification of Bioactive Molecules

Qing X. Li*

Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, Hawaii 96822, USA

09:30-10:00

Heavy Metal Contamination in Soil-Water Plant System and Their Human Health Risk Assessment in Peri-Urban Areas of Bangladesh

M. Mokhlesur Rahman*, M. Taufique Arefin

Department of Agricultural Chemistry, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

10:00-10:30

Discovery of Secondary Metabolites with Nematicidal Activity from Higher Fungi against Root-knot Nematodes

Jin-Cheol Kim*

Department of Agricultural Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of Korea

14:50-15:20

Mechanisms mediating anti-inflammatory effects of dietary bioactive compounds in obesity and insulin resistance

Naima Moustaid-Moussa*

Department of Nutritional Sciences, Obesity Research Institute, Texas Tech University, Lubbock, TX, USA


Chair: Young-Soo Keum (Konkuk University)

IS2 Environmental Sciences

June 21 (Fri), Hall B

Chair: Namhyun Chung (Korea University)


Chair: Byoung Seob Ko (Korea Institute of Oriental Medicine)

IS3 Natural Products · Bioactive Materials · Biomedical Sciences

IS3-1

16


09:30-10:00

Cannabis in California, USA

Gyunghoon Hong*

Bureau of Cannabis Control, Department of Consumer Affair, Sacramento, CA, USA

10:00-10:30

Optimization of compound mixtures via ex vivo fluorescence monitoring for improved cellular delivery and effects

Hyejung Mok*

Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea

15:20-15:50

The dangers of hidden fat: bringing intra-pancreatic fat deposition to the fore

Max S. Petrov*

School of Medicine, University of Auckland, Auckland 1023, New Zealand

09:30-10:00

Safety Issues of Food and Agricultural Products in Kazakhstan

Jannat Iskakova1*, Zhelderbayeva Makhabbat2

1Candidate of Agricultural Sciences, Associate Professor of “Food technology and safety” Department, Kazakh National Agrarian University, Kazakhstan, 2Candidate of Medical Sciences, Kazakh National Medical University by Asfendiarov, Kazakhstan



IS4 Food Sciences

June 21 (Fri), Hall C

Chair: Seung Sik Lee (Korea Atomic Energy Research Institute)

IS3-2

IS3-3

IS4-1

IS4-2

June 21 (Fri), Studio G

Chair: Jaehong Han (Chung-Ang University)

17


IS4-3 10:00-10:30

Functional thermotolerant yeasts isolated in Vietnam for ethanol and fruit wine production

Ngo Thi Phuong Dung1*, Huynh Xuan Phong1, Mamoru Yamada2, Pornthap Thanonkeo3

1Biotechnology Research and Development Institute, Can Tho University, Can Tho, Vietnam, 2Faculty of Agriculture and Graduate School of Science and Technology for Innovation, Yamaguchi University, Yamaguchi, Japan, 3Faculty of Technology, Khon Kaen University, Khon Kaen, Thailand

15:50-16:20

Anaerobic ammonium oxidizing (anammox) bacteria – from ecophysiology to practical application –

Satoshi Okabe*

Division of Environmental Engineering, Faculty of Engineering, Hokkaido University, North-13, West-8, Sapporo, Hokkaido 060-8628, Japan

09:30-10:00

Banking and Characterizing Human Gut Microbiome

Ju Huck Lee*, Byeong Seob Oh, Seung Yeob Yu, Ji-Sun Kim, Seung-Hwan Park, Se Won Kang,

Jam-Eon Park, Seung-Hyeon Choi, Kook-Il Han, Keun Chul Lee, Mi Kyung Eom, Min Kuk Suh,

Jung-Sook Lee

Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, Republic of Korea

10:00-10:30

Regulation of Plant and Human Immunity by Interaction of Salicylates with Their Target Proteins

Hyong Woo Choi*

Department of Plant Medicals, Andong National University, Andong 36729, Republic of Korea



IS5 Applied Microbiology

June 21 (Fri), Studio 3

Chair: Youngmin Kang (Korea Institute of Oriental Medicine)

IS5-1

IS5-2

IS5-3

18


10:30-11:00

Development of techniques for practical applications of multifunctional microorganism in agriculture

Jun-Kyung Park1, Sun-Il Seo1, JaeKyeong Song2, Chul-Won Lee3, Yong-Hak Kim4, Pyoung Il Kim1*1Center for Industrialization of Agricultural and Livestock Microorganisms, Jeongeup, Republic of Korea, 2Agricultural Microbiology Division, National Institute of Agricultural Sciences, RDA, Wanju, Republic of Korea, 3Department of Chemistry, Chonnam National University, Gwangju, Republic of Korea, 4Department of Microbiology, Daegu Catholic University School of Medicine, Daegu, Republic of Korea

10:40-11:10

Highly specific affinity tag based protein purification using 2B8 tag and 2B8 antibody

Kyung-Ha Lee, Seong Hee Bhoo*

Graduate School of Biotechnology, KyungHee University, Yong-in 17104, Republic of Korea

11:10-11:40

Molecular and Functional Study of Insect Odorant Receptor

Young Kun Shim1,2, Sanung Eom1, Je Jun Oh1,2, Junho Lee1*1Department of Biotechnology, Chonnam National University, Gwangju, Republic of Korea, 2Microzyme Corporation, Damyang Samanli 234, Jeonmam, Republic of Korea

11:40-12:10

Elucidating the shared and unique responses of Arabidopsis thaliana to multiple environmental stresses using meta-analysis approach

Ashutosh Singh*

Translational Bioinformatics and Computational Genomics Lab, Department of Life Sciences, Shiv Nadar University, Greater Noida, UP, India

IS5-4

S1-1

S1-2

S1-3

Symposia


Chair: Jae-Ho Shin (Kyungpook National University)

S1 Biochemistry · Molecular Biology

19


10:40-11:10

Hormetic effect of veterinary antibiotics on the enzyme activity of soil planted with Pisum sativum L.

Reep Pandi Tasho, Jae Young Cho*

Department of Bioenvironment Chemistry, Chonbuk National University, Jeonju, Republic of Korea

11:10-11:40

Methane cycling under nitrogen-limited conditions: diazotrophy as a life-strategy of methanogens and methantrophs?

Sang Yoon Kim1*, Ju-Hee Lee1, Paul L.E. Bodelier2

1Department of Bio-environmental Sciences, Sunchon National University, Suncheon, Republic of Korea, 2Netherlands Institute of Ecology (NIOO-KNAW), Department of Microbial Ecology, Wageningen, the Netherlands

11:40-12:10

Biochar as Catalytic Materials for Biorefinery Applications

Jechan Lee*

Department of Environmental and Safety Engineering, Ajou University, Suwon 16499, Republic of Korea

10:40-11:10

Imaging of β-amyloid plaques by near-infrared fluorescent probe in Alzheimer’s disease

Yong Dae Park*, Sungbeom Lee, Hyoung-Woo Bai, Byung Yeoup Chung, Seung Sik Lee

Research Division for Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 580-185, Republic of Korea

S3-1

S2-1

S2-2

S2-3


Chair: Dong Cheol Seo (Gyeongsang National University)

S2 Environmental Sciences


Chair: Hyoungwoo Bai (Korea Atomic Energy Research Institute)

S3 Natural Products · Bioactive Materials · Biomedical Sciences

20


11:10-11:40

Total Synthesis of Antibiotic Disciformycin A and B1

Yonghoon Kwon, Saskia Schulthoff, Quang Minh Dao, Conny Wirtz, Alois Fürstner*

Max-Planck-Institute for Kohlenforschung, Mülheim an der Ruhr, Germany

11:40-12:10

Relation of environmental variation to skin biophysiological parameters of Korean females

Jihye Maeng1, Gaewon Nam1,2*1Seowon Skin Research Center, Cheongju 28674, Republic of Korea, 2Departments of Biocosmetic Science, Seowon University, Cheongju 28674, Republic of Korea

10:40-11:10

Studies on volatile analysis of various foods by different extraction techniques

Yoon-Yeol Lee, Jun-Young Lee, Hee-Don Choi, Hae Won Jang*

Korea Food Research Institute, Nonsaengmyeong-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do, Republic of Korea

11:10-11:40

Anti-obesity effect of green bean fermented by lactic acid bacteria

Myoung Nim Shin1*, Yong Seob Jeong2

1Department of Bioenvironmental Chemistry, Chonbuk National University, Jeonju 567, Republic of Korea, 2Department of Food Science and Technology, Chonbuk National University, Jeonju 567, Republic of Korea

11:40-12:10

Database on flavonoids and phenolic acids in Korean agro-food resources using mass technology

Heon-Woong Kim, Min-Ki Lee, Seon-Hye Lee, Gelila Asamenew, Suji Lee, Sang Hoon Lee,

Seon Mi Yoo, Jung-Bong Kim*

National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea

S3-2

S3-3

S4-1

S4-2

S4-3

June 21 (Fri), Studio G

Chair: Jihyun Lee (Chung-Ang University)

S4 Food Sciences

21


13:30-14:00

Unraveling the DNA damage response in the radiation-resistant fungus, Cryptococcus neoformansKwang-Woo Jung1, Yeonseon Lee2, Doog-Hoon Yang2, Min-Kyu Kim1, Ho Seong Seo1,

Eun Young Huh3, Soo Chan Lee3, Sangyong Lim1*, Yong-Sun Bahn2*1Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup, Republic of Korea, 2Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Republic of Korea, 3South Texas Center for Emerging Infectious Diseases (STCEID), Department of Biology, the University of Texas at San Antonio, San Antonio, Texas, USA

14:00-14:30

Regulation of fungal development and pathogenesis through histone acetylation/deacetylation in the rice blast fungus

Jaejoon Lee, Jongjoon Lee, Taehyun Kim, Gnanendra Shanmugam, Junhyun Jeon*

Department of Biotechnology, College of Life and Applied Sciences, Yeungnam University, Gyeongsan, Gyeongbuk 38541, Republic of Korea

14:30-15:00

Therapeutic effect of extracellular matrix degradation enzyme delivered by combination engineered bacteria with anticancer drugs

Jam-Eon Park, Seung-Hyeon Choi, Ju Huck Lee, Seung-Hwan Park*

Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup 56212, Jeollabuk-do, Republic of Korea

13:30-14:00

Trait modifications using a protoplast CRISPR/Cas9 system in horticultural crops: review and progress

Geung-Joo Lee*, Luhua Tu, Saminathan Subburaj, Ka Yeon Lee, Yong Sam Jeon, Kwang Soo Park

Department of Horticultural Science, Chungnam National University, Daejeon 34134, Republic of Korea

S5-1

S6-1

S5-3

June 21 (Fri), Studio 3

Chair: Ji-Hoon Lee (Chonbuk National University)

S5 Applied Microbiology


Chair: Jae-Yean Kim (Gyeongsang National University)

S6 Agro-Bio Genome Editing

S5-2

22


14:00-14:30

Optimization of meristem maturation and shoot growth in tomato

Jung Heo1, Ryza A. Priatama1,2, Eunsong Lee1, Sukgui Oh3, Jong Hyang Bae3, Soon Ju Park1*1Department of Biological Science and Institute for Basic Science, Wonkwang University, 460 Iksandae-ro, Iksan, Jeonbuk, Republic of Korea, 2Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University, Jinju, Republic of Korea, 3Department of Horticulture Industry, Wonkwang University, Iksan 54538, Republic of Korea

14:30-15:00

Identification of Genes Involved in Trichome Development and Insect Resistance in Tomato

Jae-In Chun, Won-Ki Hong, Heejin Kim, Jin-Ho Kang*

Graduate School of International Agricultural Technology and Crop Biotechnology Institute, Seoul National University, Pyeongchang 25354, Republic of Korea

13:30-13:55

Forensic Chemistry: Review of Status and Needs

Jin Young Kim*

Forensic Genetics & Chemistry Division, Supreme Prosecutors’ Office, Seoul 06590, Republic of Korea

13:55-14:20

Identification of phytolaccosides in the biological samples from pokeweed intoxication patients using liquid chromatography-tandem mass spectrometry

Sanggil Choe1, Suncheun Kim2*1Drug and Toxicology Section, Seoul Institute, National Forensic Service, 08036, Republic of Korea, 2Fornesic Toxicology & Chemistry Division, Daejeon Institute, National Forensic Service, 34054, Republic of Korea

14:20-14:45

Military forensic science and Applied Biological Chemistry

Choong-Sik Lee*

Division of Toxicology and Chemistry, Criminal Investigation Command, Ministry of National Defense, Seoul 04383, Republic of Korea

S6-2

S6-3

S7-1

S7-2

S7-3


Chair: Joon-Kwan Moon (Hankyong National University)

S7 Forensic Science

23


14:45-15:10

Quantitative Tandem Mass Spectrometry for Simultaneous Analysis of Pesticide Multiresidues in Human Serum and Urine

Yongho Shin1, Jonghwa Lee2, Jiho Lee2, Junghak Lee2, Eunyoung Park2, Hye Suk Lee1,

Jeong-Han Kim2*1Drug Metabolism and Bioanalysis Laboratory, College of Pharmacy, The Catholic University of Korea, Bucheon-si, Gyeonggi-do 14662, Republic of Korea, 2Pesticide Chemistry and Toxicology Laboratory, Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea

13:30-14:00

A Key to the Success of PLS Implementation: Authorative Registration of Pesticides Using Minor Crops

Su-Myeong Hong*, Hee-Dong Lee, Sang-Bum Lee

Department of Agro-material Assessment, National Institute of Agricultural Sciences, RDA, Wanju 55365, Republic of Korea

14:00-14:30

Pesticide MRL Setting and PLS Enforcement in Korea

Jin Sook Kim*

Residues and Contaminants Standard Division, Ministry of Food and Drug Safety, Osong Health Technology Administration 187, Republic of Korea

14:30-15:00

Suggestion of Solutions to Problems Caused by Implementation of Positive List System

Se-Yeon Kwak, Jang-Eok Kim*


S7-4

S8-1

S8-2

S8-3


Chair: In Seon Kim (Chonnam National University)

S8 Pesticide Positive List System

24


16:00-16:20 학술상

Targeting of cathepsin C in colorectal cancer cells

Sun Chul Kang*


16:20-16:40 기창(基倉)과학상

NITROGEN RESPONSE DEFICIENCY 1 positively regulates NRT1.1 for optimized growth in response to altered nitrate availability in Arabidopsis

Won Je Lee1, Hai An Truong1, Cao Sơn Trịnh1, Seokjin Lee1, Suk-Whan Hong2, Hojoung Lee1*1Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong 5-ga, Seongbuk-gu, Seoul 02841, Republic of Korea, 2Department of Molecular Biotechnology, College of Agriculture and Life Sciences, Bioenergy Research Center, Chonnam National University, Gwangju 61186, Republic of Korea

Award Lectures

June 21 (Fri), Grand Ballroom

Chair: Sung-Eun Lee (Kyungpook National University)

AL-1

AL-2

25


PBM Biochemistry · Molecular Biology

PNB Natural Products · Bioactive Materials · Biomedical Sciences

PES Environmental Sciences

PFS Food Sciences

PAM Applied Microbiology

Poster Category

PBM PNB PES PFS PAM

I#1~51 #1~65 #1~37 #1~21 #1~6June 20 (Thu)

17:00-18:30

II

#52~102 #66~130 #38~75 #22~42 #7~11June 21 (Fri)15:00-16:00

Place Convention Hall

Poster Presentation I & II

DateSession


PBM-1 Meloidogyne incognita transcriptome mining during various stages of its development Chinreddy Subramanyam Reddy, Vimalraj Mani, Soyoung Park, Joon-Soo Sim, Chang-Muk Lee,

Bum-Soo Hahn*

Metabolic Engineering Division, Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Korea

PBM-2 Profiling and characterization of miRNA target genes in root-knot nematode (Meloidogyne incognita)

Chinreddy Subramanyam Reddy, Vimalraj Mani, Soyoung Park, Joon-Soo Sim, Chang-Muk Lee,

Bum-Soo Hahn*


Poster Presentation

26


PBM-3 Qualitative analysis of cationic metabolites in tomato germplasm using capillary electrophoresis mass spectrometry

Seon-Kyeong Lee, Chinreddy Subramanyam Reddy, Vimalraj Mani, Joon-Soo Sim,

Chang-Muk Lee, Soyoung Park, Su-Yeon Kim, Mahipal Singh Kesawat, Bum-Soo Hahn*


PBM-4 Highly specific affinity tag based protein purification using 2B8 tag and 2B8 antibody Kyungha Lee, Seong Hee Bhoo*

Graduate School of Biotechnology, KyungHee University

PBM-5 Phylogenetic relationships of the Cultivated Mulberry (Rosales: Moraceae) in Korean Wan Taek Ju*, O Chul Kwon, Hyun Bok Kim, Ji Hae Lee

Sericultural & Apicultural Materials Division, National Academy of Agricultural Science

PBM-6 Development, Identification and Validation of Specific Markers for Antler-shaped Ganoderma lucidum

O Chul Kwon*, Wan Taek Ju, Hyun Bok Kim, Ji Hae Lee


PBM-7 Myb-like protein, NID1, involved in nitrogen signaling pathway to mediate optimal root development in Arabidopsis

Won Je Lee1, Hojoung Lee2* 1Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong 5-ga, Seongbuk-gu, Seoul 02841, Republic of Korea, 2Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong 5-ga, Seongbuk-gu, Seoul 02841, Republic of Korea

PBM-8 Assessment of synthetic hexaploid wheats in response to heat stress and lead rust infection for the improvement of wheat production

Hai An Truong1, Won Je Lee1, Masahiro Kishii2, Suk-Whan Hong3, Chon-Sik Kang4,

Byung Cheon Lee5, Hojoung Lee1* 1Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong 5-ga, Seongbuk-gu, Seoul 02841, Republic of Korea, 2Global Wheat Program, International Maize and Wheat Improvement Center (CIMMYT), Carretera México-Veracruz Km. 45, El Batán, Texcoco, México, 3Department of Molecular Biotechnology, College of Agriculture and Life Sciences, Bioenergy Research Center, Chonnam National University, Gwangju 61186, Republic of Korea, 4Crop Breeding Division, National Institute of Crop Science, RDA, Wanju 55365, Republic of Korea, 5Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong 5-ga, Seongbuk-gu, Seoul 02841, Republic of Korea

27


PBM-9 Characterization of two leaf rust-resistant Aegilops tauschii accessions for developing synthetic wheat

Cao Son Trịnh1, Aro Lee1, Won Je Lee1, Minseo Kim1, Hyeri Lee1, Duleepa Pathiraja1,

In-Geol Choi1, Namhyun Chung1, Chon Sik Kang2, Byung Cheon Lee1, Hojoung Lee1* 1Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong 5-ga, Seongbuk-gu, Seoul 02841, Republic of Korea, 2Crop Breeding Division, National Institute of Crop Science, RDA, Wanju 55365, Korea, Republic of Korea

PBM-10 Bacillus subtilis strain Y16 promotes nitrate reductase activity and elicits enhanced growth performance in Arabidopsis

Seokjin Lee1, Chan Young Jeong1, Won Je Lee1, Cao Sơn Trịnh1, Hai An Truong1,

Suk-Whan Hong2, Hojoung Lee1* 1Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong 5-ga, Seongbuk-gu, Seoul 02841, Republic of Korea, 2Department of Molecular Biotechnology, College of Agriculture and Life Sciences, Bioenergy Research Center, Chonnam National University, Gwangju 61186, Republic of Korea

PBM-11 Molecular signaling axis in bleomycin-induced pulmonary pathogenesis in A549 cells Karthika Muthuramalingam1, Changmin Choi1, Dharanibalan Kasiviswanathan1, Youngmee Kim1*,

Moonjae Cho2* 1Department of Biochemistry, School of Medicine, Jeju National University, Jeju 63243, 2Department of Biochemistry, School of Medicine, Institute of Medical Science, Jeju National University, Jeju 63241

PBM-12 Regulation of photosynthetic activity by an Isoform of the plastid RNA polymerase-associated protein FSD3

Geupil Jang*, Sangyool Lee

School of Biological Sciences and Technology, Chonnam National University

PBM-13 Production of anti-MERS-CoV S protein monoclonal antibody for inhibition of MERS-CoV infection by epitope peptide-CpG-DNA-liposome complex without carriers

Byoung Kwon Park1, Sony Maharjan1, Su In Lee1, Jinsoo Kim1, Joon-Yong Bae2, Man-Seong Park2,

Younghee Lee3, Hyung-Joo Kwon4* 1Center for Medical Science Research, Hallym University College of Medicine, 2Department of Microbiology, Korea University, 3Department of Biochemistry, Chungbuk National University, 4Department of Microbiology, Hallym University College of Medicine

28


PBM-14 Production of bacteria-reactive IgM antibodies by CpG-DNA to enhance phagocytic activity against Staphylococcus aureus infection

Te Ha Kim1, Dong Bum Kim2, Hee Su Lee1, Min Hyung Kwak1, Avishekh Gautam1,

Madhav Akauliya1, Joongwon Park1, Jinsoo Kim1, Hanseul Lee1, Sang Kyu Park3, Younghee Lee3,

Hyung-Joo Kwon1* 1Department of Microbiology, Hallym University College of Medicine, 2Center for Medical Science Research, Hallym University College of Medicine, 3Department of Biochemistry, Chungbuk National University

PBM-15 Intramitochondrial transfer and engineering of mammalian mitochondrial genomes in yeast

Young Geol Yoon1*, Michael Koob2 1Department of Biomedical Science, Jungwon University, 2Institutes for Translational Neuroscience & of Human Genetics, Department of Laboratory Medicine and Pathology, University of Minnesota

PBM-16 Characterization and Antifungal Activity of Chitinase Producing Bacteria Philip Ahn, Woo-Jin Jung*

Department of Agricultural Chemistry, Institute of Environmentally-Friendly Agriculture (IEFA), College of Agriculture and Life Sciences Chonnam National University

PBM-17 The Current Species Compotent of Bemisia tabaci in China and South Korea Chenliang Guo1, Bueyong Park2, Gwan- Seok Lee2, Dong Chu1, Yong- Zhe Zhu1, In- Hong Hong2* 1Key Lab of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, China, 2Department of Crop Life safety Division of Crop Protection, National Institute of Agricultural Sciences, Korea

PBM-18 Identification and Characterization of Saikosaponins as Antagonists of Transient Receptor Potential A1 channel

Yongmun Choi*, Gyeong Been Lee, Jungeun Yang, Jongsuk Lee, Changon Seo

Biomaterials R&D Team, Gyeonggido Business & Science Accelerator Bio Center

PBM-19 Molecular genetic characterization of orevactaene biosynthesis in Epicoccum nigrum and its application on the production of its derivatives

Yoonji Lim, Eun-ha Choi, Hyung-Jin Kwon*

Department of Biological Sciences and Bioinformatics, Myongji University

29


PBM-20 Diplacone isolated from paulownia tomentosa fruits induces MPT-mediated necrotic cell death in A549 cells

Myung-Ji Kang1,2, Hyung Won Ryu1, Eun-Bin Kwon1,2, Su Ui Lee1, Sei-Ryang Oh1, Dong-Oh Moon3,

Mun-Ock Kim1* 1Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 2College of pharmacy, Chungbuk National University, 3Department of Biology Education, Daegu University

PBM-21 RCVpW-based insecticide resistance monitoring and mechanism of spinosad resistance in Thrips palmi

Ju Hyeon Kim1, Min Ju Kim2*, Kyungmun Kim2*, Tian-Bo Ding3*, In Hong Jeong4, Deok Ho Kwon1*,

Si Hyeock Lee2 1Research Institute of Agriculture and Life Sciences, Seoul National University, 2Department of Agricultural Biotechnology, Seoul National University, 3College of Plant Health and Medicine, Qingdao Agricultural University, 4Division of Crop Protection, National Institutes of Agricultural Science, Republic Development Administration

PBM-22 Characterization of venom components in 14 Aculeate bees/wasps via venom gland transcriptome analysis

Kyungjae Andrew Yoon1, Do Eun Lee2*, Woo Young Bang3*, Chang Hwan Bae3*, Joo-Hong Yeo3*,

Young Ho Koh4*, Si Hyeock Lee1* 1Research Institute for Agriculture and Life Sciences, Seoul National University, 2Department of Agricultural Biology, Seoul National University, 3National Institute of Biological Resources (NIBR), Environmental Research Complex, 4Ilsong Institute of Life Science, Hallym University

PBM-23 Stimulating DDX3 expression by Serotonin 5-HT receptor 7 through phosphorylation of p53 via the AC-PKA-ERK signaling pathway

Khoa Nguyen, Junho Lee*

Biotechnology, Chonnam National University

PBM-24 Molecular determinants of α3β4 nicotinic acetylcholine receptors inhibition by triterpenoids

Sanung Eom, Junho Lee*


PBM-25 Hyaluronic acid production with galactose and glucose in Escherichia coli W3110Seong Woo Kwon, Ji Eun Woo, Yu-Sin Jang*

Institute of Agriculture & Life Science (IALS), Department of Agricultural Chemistry and Food Science Technology, Division of Applied Life Chemistry, Gyeongsang National University

30


PBM-26 HR-MAS NMR, UPLC-QTOF/MS and GCxGC-TOF/MS-based metabolomics study for age discrimination of ginseng

Dahye Yoon1, Bo-Ram Choi1, Seohee Ma2, Jae Won Lee1, Ick-Hyun Jo1, Young-Seob Lee1,

Geum-Soog Kim1, Suhkmann Kim2, Dae Young Lee1* 1Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, 2Department of Chemistry, Center for Proteome Biophysics and Chemistry Institute for Functional Materials, Pusan National University

PBM-27 Differential association of BsSIG5 with multiple regions in plastid genome depending on light condition in Bienertia sinuspersici

Yen Le Thi, Lorrenne Caburatan, Eunsun Park, Hwan Namgoong, Joonho Park*

Department of Fine Chemistry, Seoul National University of Science and Technology

PBM-28 Identification of plant host factors associated with cucumber mosaic virus infection by proteomic and transcriptomic approaches

Soo-Jung Han, Jang-Kyun Seo*

Department of International Agricultural Technology, Seoul National University

PBM-29 Biosynthesis of N-2-hydroxybenzoyl tryptamine using engineered Escherichia coli Songyi Kim, Joong-Hoon Ahn*

Department of Bioscience and Biotechnology, Konkuk University

PBM-30 Microbial synthesis of chiral p-coumaroyl glycerol Min Kyung Song, Geun Young Sim, Su Jin Lee, Bong-Gyu Kim, Mihyang Kim, Taegum Lee,

Youhoon Chong, Joong-Hoon Ahn*


PBM-31 Synthesis of methylated anthranilate derivatives using engineered strains of Escherichia coliHye Lim Lee, Songyi Kim, Eun Ji Kim, Da Ye Han, Bong-Gyu Kim, Joong-Hoon Ahn*


PBM-32 Synthesis of Acridone using engineered Escherichia coli Gyu Sik Choi, Hye Jeong Choo, Joong Hoon Ahn*


31


PBM-33 Synthesis of chlorogenic acid from glucose in Escherichia coli Hye-Lime Lee, Joong-Hoon Ahn*


PBM-34 Biosynthesis of 2,4-Dihydroxyquinoline in engineered Escherichia coli Hye Jeong Choo, Joong Hoon Ahn*


PBM-35 Bioinformatic approaches to identify novel gene targets responsible for adriamycin resistance in A549 cells

Dharanibalan Kasiviswanathan1, Jeong Yong Moon2, Somi Kim Cho2,3,4*, Moonjae Cho1,5* 1Department of Biochemsitry, School of Medicine, Jeju National University, Jeju 63243, Korea, 2Subtropical/Tropical Organism Gene Bank, Jeju National University, Jeju 63243, Korea, 3Interdisciplinary Graduate Program in Advanced Convergence Technology and Science, Jeju National University, Jeju 63243, Korea, 4Faculty of Biotechnology, College of Applied Life Sciences, SARI, Jeju National University, Jeju 63243, Korea, 5Institues of Medical Sciences, Jeju National University, Jeju 63243, Korea

PBM-36 Dual functions of Arabidopsis thaliana ascorbate peroxidase 1 are related to structural conformation

Shubhpreet Kaur1,2, Prapti Prakash1,2, Moon-Soo Chung1, Sungbeom Lee1,2, Jin-Hong Kim1,

Seung Sik Lee1,2* 1Research Division for Biotechnology, Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI), 2Department of Radiation Science and Technology, University of Science and Technology (UST)

PBM-37 Synthesis of 4-hydroxybenzoic acid derivates in Escherichia coliHan Kim, Geun Yong Sim, Songyi Kim, Joong-Hoon Ahn*


PBM-38 Genome-wide Identification and Functional Characterization of Drought-Responsive Long Noncoding RNAs (DRIL) in Rice

Nuri Oh1, Jimin Lee2*, Daeheon Jang2*, Choonkyun Jung1,2* 1Institutes of Green Bio Science and Technology, Seoul National University, 2Graduate School of International Agricultural Technology, Seoul National University

32


PBM-39 Induction of apoptosis by mono(2-ethylhexyl) phthalate via enhanced generations of nitric oxide and reactive oxygen species in wild-type and p53-null human lymphoblastoid cells

Eun Mi Park, Min Young Kim*

Toxicology Laboratory, Major in Biomaterials, Jeju National University, Jeju 63243, Korea

PBM-40 Development of a film-based immunochromatographic microfluidic device for malaria diagnosis

Yong Tae Kim, Jihye Choi, Heungsop Shin*

Department of Chemical Engineering & Biotechnology, Korea Polytechnic University

PBM-41 Mutations on a turn and loop other than in ligands of copper in amicyanin affect the volume of active site

Youngkook Moon1, Moonsung Choi2*, Sooim Shin1,3* 1Interdisciplinary Program of Bioenergy and Biomaterials Graduate School, College of Engineering, Chonnam National University, Gwangju 61186, Republic of Korea, 2Department of Optometry, College of Energy and Biotechnology, Seoul National University of Science and Technology, Seoul 01811, Republic of Korea, 3Department of Biotechnology and Bioengineering, College of Engineering, Chonnam National University, Gwangju 61186, Republic of Korea

PBM-42 Stereolithographic Printing of Microfluidic Vascular Chip using Biocompatible Hydrogels

Eun-Jeong Choi, Heungsop Shin*, Yong Tae Kim*


PBM-43 Morphological and molecular characterization of mutants related to distorted trichomes in tomato

Jae-In Chun1, Won-Ki Hong1, Seong-Min Kim1, Jin-Ho Kang1,2* 1Graduate School of International Agricultural Technology and Crop Biotechnology Institute, Seoul National University, 2Institute of GreenBio Science and Technology, Seoul National University

PBM-44 Investigation of physio-molecular response to water-deficit stress in Korean F1 maize (Zea mays L.) hybrids

Won Min Jeong, Dong Kyu Jeong, Dong Yeol Lee, Young Min Goo, Young Sook Kil, Seung Mi Sin,

Hyeong Hwan Lee, Sang Gon Kim*

Research & Development Team, Gyeongnam Oriental Anti-Aging Institute

33


PBM-45 Towards reducing the immunogenic potential of wheat flour: omega gliadins encoded by the D genome of hexaploid wheat may also harbor epitopes for the serious food allergy WDEIA

Jong-Yeol Lee1*, Sun-Hyung Lim1, Susan B Altenbach2 1National Institute of Agricultural Science, RDA, Jeonju 54874, Korea, 2USDA-ARS, Western Regional Research Center, 800, Buchanan Street, Albany, CA 94710, USA

PBM-46 Proteomic Profiling and Epitope Analysis of the Complex α-, γ-, and ω-Gliadin Families in a Korean Wheat Variety “Keumkang”

Sewon Kim1, Kyoungwon Cho2, Susan B Altenbach3, Sun-Hyung Lim1, Jong-Yeol Lee1* 1National Institute of Agricultural Science, RDA, Jeonju 54874, Korea, 2Department of Biotechnology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Korea, 3USDA-ARS, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710, USA

PBM-47 Overexpressing wheat low-molecular-weight glutenin subunits in rice (Oryza sativa L. japonica cv. Koami) seeds

Su-Bin Lee1, Kyoungwon Cho2, Sun-Hyung Lim1, Jong-Yeol Lee1* 1National Institute of Agricultural Science, RDA, Jeonju 54874, Korea, 2Department of Biotechnology, Collge of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Korea

PBM-48 Cellular Localization of Wheat High Molecular Weight Glutenin Subunits in Transgenic Rice Grain

Jae-Ryeong Sim1, Kyoungwon Cho2, Sun-Hyung Lim1, Jong-Yeol Lee1* 1National Institute of Agricultural Science, RDA, Jeonju 54874, Korea, 2Department of Biotechnology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Korea

PBM-49 Cytoprotective effects of Centipedegrass extract against oxidative stress in NIH-3T3 fibroblasts

Dongho Bak1, Seong Hee Kang1, Kwangwoo Jung1, Kin Kyu Kim1,2, Byung Yeoup Chung1,

Seung Sik Lee1,2* 1Research Division for Biotechnology, Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI), 2Department of Radiation Science and Technology, University of Science and Technology (UST)

PBM-50 Acacetin stimulates GLUT4 translocation through insulin-independent pathwayEun-Bin Kwon1,2, Myung-Ji Kang1,2, Hyung Won Ryu1, Seoghyen Lee3, Mi Kyeong Lee2, Su Ui Lee1,

Mun-Ock Kim1*, Sei-Ryang Oh1* 1Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 2College of Pharmacy, Chungbuk National University, 3Department of Bioscience Biotechnology, Chungnam National University

34


PBM-51 Genome-wide Comparative Analysis of MicroRNAs and Disease-resistant Genes in Solanaceae Plants

Jeongyeon Yoon1, Taewook Kim1, Eunyoung Seo2, Taeyoung Um1, Taehyeon Park1, Doil Choi2,

Chanseok Shin1* 1Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea, 2Department of Plant Science, Seoul National University, Seoul 08826, Republic of Korea

PBM-52 LegExpress: An interactive bioinformatic platform for cross-species translation of transcriptome data in legumes

Joo-Seok Park1, Jin-Hyun Kim2, Yoram Choi1, Min-Gyun Jeong1, Hong-Kyu Choi3* 1Department of Applied Bioscience, Dong-A University, 2Department of Medical Bioscience, Dong-A University, 3Department of Molecular Genetics, Dong-A University

PBM-53 Identification of Damulin B in vitro metabolism using metabolomics technology Ga-Hyun Lee1, Tae Lin Huh2, Kwang-Hyeon Liu1* 1BK21 Plus Multi-Omics Based Creative Drug Research Team, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 41566, Korea, 2School of Life Science and Biotechnology, Kyungpook National University, Daegu 41566, Korea

PBM-54 LegCompara: a real time-responsible bioinformatic platform for the comparative genome analysis of legume genomes

Jin-Hyun Kim1, Joo-Seok Park2, Yoram Choi2, Min-Gyun Jeong2, Hong-Kyu Choi3* 1Department of Medical Bioscience, Dong-A University, 2Department of Applied Bioscience, Dong-A University, 3Department of Molecular Genetics, Dong-A University

PBM-55 Ginseng-derived two CYP genes functions on plant growth and phenylurea herbicide tolerance

Jin Hoon Jang, Soo Kwang Yim, Ok Ran Lee*

Department of Applied Plant Science, College of Agriculture and Life Science, Chonnam National University

PBM-56 Involvement of chalcone synthase genes in the rice phytoalexin biosynthesis Kyungha Lee, Hye Lin Park, Seong Hee Bhoo, Man-Ho Cho*

Graduate School of Biotechnology, Kyung Hee University

35


PBM-57 Genome-wide association study dissects seed pigmentation-associated genes and loci in soybean genome

Jin-Hyun Kim1, Joo-Seok Park2, Yoram Choi2, Min-Gyun Jeong2, Hong-Kyu Choi3* 1Department of Medical Bioscience, Dong-A University, 2Department of Applied Bioscience, Dong-A University, 3Department of Molecular Genetics, Dong-A University

PBM-58 Insight about variations in multiple copies of bacterial 16S rRNA genes may aid in species identification

Jerald Conrad Ibal, Chang Eon Park, Yeong-Jun Park, Min-Chul Kim, Young Jae Jo,

Huy Quang Pham, Gi-Ung Kang, Sajjad Ahmad, Jae-Ho Shin*

School of Applied Biosciences, Kyungpook National University

PBM-59 Copper incorporation energetics in amicyanin’s folding mechanism Seounghun Jeoung1, Kyungil Bang2, Sooim Shin1,2*, Moonsung Choi3* 1Interdisciplinary Program of Bioenergy and Biomaterials Graduate School, College of Engineering, Chonnam National University, 2Department of Bioengineering and Biotechnology, College of Engineering, Chonnam National University, 3Department of Optometry, College of Energy and Biotechnology, Seoul National University of Science and Technology

PBM-60 Effect of S219V mutation on activity of Tobacco Etch Virus (TEV) protease Heejin Nam1, Sooim Shin1,2* 1Interdisciplinary Program of Bioenergy and Biomaterials Graduate School, College of Engineering, Chonnam National University, 2Department of Biotechnology and Bioengineering, College of Engineering, Chonnam National University

PBM-61 BRCA1 regulates RNA polymerase II promoter-proximal pause release through modulating topoisomerase IIb stability

Heeyoun Bunch*, Sanghwa Lee

Applied Biosciences, Kyungpook National University

PBM-62 Development of fluorescence-based biosensor using fRMsr for detecting accumulated damage by oxidative stress

Seahyun Kim, Hae Min Lee, Aro Lee, Bung Cheon Lee*

College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea

36


PBM-63 Structural Insights and Kinetic Characterization of MICAL3: Comparison to MICAL1 by their CH Domains

Yeon Jin Roh, Byung Cheon Lee*

College of Life Sciences & Biotechnology, Korea University, Seoul 02841, Republic of Korea

PBM-64 Evalutaion of Leaf Rust Resistance and the characteristic in Korean wheat Minseo Kim, Byung Cheon Lee*


PBM-65 Comparison of diosgenin related gene expression and content on organs of Trigonella foenum-graecum L.

Do Yeon Kwon1, Sang Un Park2* 1Smart Farm Resesarch Center, KIST Gangneung Institute of Natural Products, 2Department of Crop Science, Chungnam National University

PBM-66 5’, 8”-Biapigenin is selective CYP2C8 and CYP2C9 inhibitor in human liver microsomes So-Young Park*, Su-Nyeong Jang, Kwang-Hyeon Liu

BK21 Plus KNU Multi-Omics Based Creative Drug Research Team, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National, Kyungpook National University, Daegu 41566, Korea

PBM-67 Enhancement of Production of High-value Benzaldehyde Chemicals from Lignocellulosic Biomass by Thermoalkaliphilic Laccase, CtLac

Youri Yang, Sunil Ghatge, Woo-Young Song, Tae-Young Kim*, Hor-Gil Hur*

School of Earth Sciences and Environmental Engineering, Gwangju Institute of Science and Technology (GIST)

PBM-68 Lapathoside A Isolated from Fagopyrum esculentum Induces Cell Death in Human Pancreatic Cancer Cells

Mi Sook Kang1, Young-Min Ham2, Song-I Han3, Dae-Ju Oh2, Yong-Hwan Jung2, Ui Hyeon Oh1,

Da-Hye Kim1, Cheol Lee1, Dahye Jeong1, Jae Hoon Kim1,3* 1Department of Biotechnology, College of Applied Life Science, Jeju National University, 2Jeju Biodiversity Research Institute, Jeju Technopark, 3Subtropical/Tropical Organism Gene Bank, Jeju National University

PBM-69 2-Methoxy-4-vinylphenol attenuates migration of human pancreatic cancer cell via blocked of FAK and AKT signaling

Da-Hye Kim1, Jungwhoi Lee2, Boram Go3, Song-I Han2, Ui-Hyeon Oh1, Cheol Lee1, Da Hye Jeong1,

Mi Sook Kang1, Jae Hoon Kim1,2* 1Department of Biotechnology, College of Applied Life Science, Jeju National University, 2Subtropical/Tropical Organism Gene Bank, Jeju National University, 3Jeju Biodiversity Research Institute, Jeju Technopark

37


PBM-70 Effects of Toxicodendron succedaneum extracts on Differentiation and Adipogenesis in 3T3-L1 preadipocytes

Cheol Lee1, Ui Hyeon Oh1, Da Hye Jeong1, Da-Hye Kim1, So-Mi Kang1, Mi Sook Kang1,

Song-I Han2, Jae Hoon Kim1,2* 1Department of Biotechnology, College of Applied Life Science, Jeju National University, 2Subtropical/Tropical Organism Gene Bank, Jeju National University

PBM-71 The neuroprotective potentiation of the ethanol extract from Styrax japonica in HT22 mouse hippocampal cells

Da Hye Jeong1, So-Mi Kang1,2, Song-I Han3, Da-Hye Kim1, Ui Hyeon Oh1, Cheol Lee1,

Mi Sook Kang1, Jae-Hoon Kim1,3* 1Department of Biotechnology, College of Applied Life Science, Jeju National University, Jeju 63243, Republic of Korea, 2Quegen Biotech Co., Ltd., Jeju-Si, Jeju-Do, Korea, 3Subtropical/Tropical Organism Gene Bank, Jeju National University, Jeju 63243, Republic of Korea

PBM-72 Eriodictyol induced apoptosis through the PI3K/Akt pathway in human pancreatic cancer cells

Ui-Hyeon Oh1, Jungwhoi Lee2, Song-I Han2, Da-Hye Kim1, Da Hye Jeong1, Cheol Lee1,

Mi Sook Kang1, Jae Hoon Kim1,2* 1Department of Biotechnology, College of Applied Life Science, Jeju National University, 2Subtropical/Tropical Organism Gene Bank, Jeju National University

PBM-73 Identification of Two Cytochrome P450 Genes Involved in 20-Hydroxyecdysone Biosynthesis of Spinach (Spinacia oleracea L.)

Viet Dang Cao1,2, Jiwon Kim1, Key-Zung Riu1, Chang Sook Kim1, Kyung-Hwan Boo1,2* 1Department of Biotechnology, College of Applied Life Science (SARI), Jeju National University, Jeju 63243, Republic of Korea, 2Subtropical/Tropical Organism Gene Bank, Jeju National University, Jeju 63243, Republic of Korea

PBM-74 Pathogenic Characters of Pseudomonas tolaasii Strains can be Changed during the Obtaining Phage Resistance

Yeong-Bae Yun, Young-Kee Kim*

Department of Environmental and Biological Chemistry, Chungbuk National University

PBM-75 Assessment of Hfq-dependent sRNAs on the expressions of individual multidrug-efflux pump in Escherichia coliYun Son, Kwang-Sun Kim*


38


PBM-76 Establishment of Extract Method for Mepiquat chloride in Paprika and Potato with LC-MS/MS

Min-Seok Oh1*, Sujin Baek1, Hyun Ho Noh1, A. K. M. Mydul Islam1, Jin-Ho Ro1, Danbi Kim1,

Hyeyoung Kwon2, Byeong-Chul Moon1 1Department of Agro-food Safety, National Institute of Agriculture Sciences, Rural Development Administration, 2Planing and Coordination Bureau, Rural Development Agency

PBM-77 Homology-directed repair (HDR)-based genome editing in tomato Jae-Yean Kim1*, Tien Van Vu1, Jihae Kim2, Doan Thi Hai Duong2 1Division of Applied Life Science (BK21 Plus program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 2Division of Applied Life Science (BK21 Plus Program), Gyeongsang National University

PBM-78 Ginsenoside Rc is a new selective UGT1A9 inhibitor in human liver microsomes and recombinant human UGT isoforms

Su-Nyeong Jang*, Hyunyoung Lee, Jae-Kyung Heo, Ga-Hyun Lee, So-Young Park, Hyun-Ji Kim,

Kwang-Hyeon Liu

BK21 Plus KNU Multi-Omics Based Creative Drug Research Team, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 41566, South Korea

PBM-79 Characterization of PtrGATA33 related to secondary cell wall biosynthesis in Arabidopsis Jooeun Lee, Hyeonjung Jung, Won-Chan Kim*


PBM-80 PtrGATA18 regulates master regulator of secondary cell wall biosynthesis in Arabidopsis thalianaA-Hyeon Kang, Ki Hwan Lim, Ki Hwan Kim, Won-Chan Kim*


PBM-81 Biological synthesis of genistein in Escherichia coli Jeong-Hyeon Lim, Hye-Lyeong Noh, Bong-Gyu Kim*

Department of Forest Resources, Gyeongnam National University of Science and Technology

PBM-82 Functional Characterization of PAMP-responsive long noncoding RNAs in Arabidopsis Jimin Lee1, Nuri Oh2, Daeheon Jang1, Choonkyun Jung1,2* 1Graduate School of International Agricultural Technology, Seoul National University, Pyeongchang, Republic of Korea, 2Crop Biotechnology Institute, Institutes of Green Bio Science and Technology, Seoul National University, Pyeongchang, Republic of Korea

39


PBM-83 Fluorescent Adenovirus-Assisted STreamlined-Homology Directed Repair system for improved gene editing in cell lines

Hyeongseok Kim, Jisun Lee, Seungah Nam, Heungsop Shin*

Department of Chemical Engineering and Biotechnology, Korea Polytechnic University

PBM-84 A TMT-based proteome analysis of plasma membrane and cytosolic fractions highlight the MSP1 induced signaling in rice leaves

Ravi Gupta, Cheol Woo Min, Yoo Ju Soon, Jeong Woo Jang, Gi-Hyun Lee, Sun Tae Kim*

Department of Plant Bioscience, Pusan National University

PBM-85 Yield optimization with sp mutant alleles under determinate tomato Yong Jun Kim1, Min Sung Kang1, Jung Heo1, Suk Gui Oh2, Jong Hyang Bae2, Soon Ju Park1* 1Department of Biological Science and Institute for Basic Science, Wonkwang university 460 Iksandae-ro, Iksan, Jeonbuk, Korea, 2Department of Horticulture Industry, Wonkwang University, Iksan 54538, Korea

PBM-86 Bioconversion of resveratrol to δ-viniferin by grapevine peroxidases Sung-Chul Park, Jang Won Pyun, Yu Jeong Jeong, Cha Young Kim, Jae Cheol Jeong*

Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology

PBM-87 SMILE regulates iron metabolism through inhibition of BMP6-mediated hepcidin expression

Yu-Ji Kim, Bo-Eun Kim, In-Young Kim, Don-Kyu Kim*

Department of Biotechnology, Chonnam National University

PBM-88 Melatonin regulates hepcidin via JNK signaling pathwayWoo-Ram Park, Byung-Yoon Choi, Don-Kyu Kim*


PBM-89 Characterization of PagAux/IAA1 gene in Poplar (Populus alba x P. glandulosa) Jiwon Choi, Eun-Kyung Bae*

Forest Biotechnology Division, National Insitute of Forest Science

PBM-90 The Phenotype Data Collection of Rice Insertion Mutants using Retrotransposon Kyong Mi Jun1, Joung Sug Kim2, Songhwa Chae2, Gang-Seob Lee3, Baek Hie Nahm1,2* 1Plant Molecular Genetics Institute, GreenGene Biotech Inc., 2Department of Biosciences and Bioinformatics, Myongji University, 3National Academy of Agricultural Science, Rural Development Administration

40


PBM-91 Sargachromanol I and G from Sargassum siliquastrum as potent acetylcholinesterase inhibitors

Hoon Kim1*, Jongmin Oh1, Myung-Gyun Kang2, Jae Pil Lee1, Joon Yeop Lee3, Seung Cheol Baek1,

Hyun Hee Leem3, Daeui Park2, Myoung-Lae Cho3 1Department of Pharmacy, and Research Institute of Life Pharmaceutical Sciences, Sunchon National University, Suncheon 57922, Republic of Korea, 2Department of Predictive Toxicology, Korea Institute of Toxicology, Daejeon 34114, Republic of Korea, 3National Development Institute of Korean Medicine, Gyeongsan 38540, Republic of Korea

PBM-92 Divaricatic acid of Evernia mesomorpha as an antimicrobial agent for methicillin-resistant Staphylococcus aureus

Hoon Kim1*, Jong Min Oh1, Yi Jeong Kim2, Hyo-Seung Gang2, Hyong-Ho Ha1 1Department of Pharmacy, and Research Institute of Life Pharmaceutical Sciences, Sunchon National University, Suncheon 57922, Republic of Korea, 2Department of Agricultural Chemistry, Sunchon National University, Suncheon 57922, Republic of Korea

PBM-93 Characterization and application of Micro-Tom Suppressor of Dwarf Mutant Sujeevan Rajendran1*, Jung Heo1, Ryza Aditya Priatama2, Sukgui Oh3, Jong Hyang Bae3,

Soon Ju Park1* 1Division of Biological Sciences and Institute for Basic Science, Wonkwang University, Iksan 54538, Republic of Korea, 2Division of Applied Life Science, Plant Molecular Biology & Biotechnology Research Center (PMBBRC), Gyeongsang National University, Jinju 52828, Republic of Korea, 3Department of Horticulture Industry, Wonkwang University, Iksan 54538, Republic of Korea

PBM-94 ASYMMETRIC LEAVES 1 induces the shading avoidance response Young Hun Song*, Dae-Yeon Hwang

Department of Life Sciences, Ajou University

PBM-95 Interaction of oxidized p-phenylenediamine with lysozyme by spectroscopic methods Semi Kim, Sohyun Kim, Moonsung Choi*

Department of Optometry, College of Energy and Biotechnology, Seoul National University of Science and Technology

PBM-96 Inhibitory effect of cobalt oxidation induced by ROS on conformation and antibacterial activity of lysozyme

Sohyun Kim, Ji Hye Ahn, Moonsung Choi*


41


PBM-97 A Study of thermodynamic parameters of Methylisothiazolinone and lysozyme by spectroscopy and isothermal titration calorimetry

Eunji Oh, Jina Park, Moonsung Choi*


PBM-98 Inhibitory Effects of Sulfacetamide against the Lysozyme Enzymatic Activity Jihye Ahn, Sujin Park, Moonsung Choi*


PBM-99 Analysis of bypass electron transfer pathways in Co2+-loaded 6His-tagged Ca2+-depleted MauG

Seungjin An1, Youngkook Moon2, Moonsung Choi1*, Sooim Shin2* 1Department of Optometry, College of Energy and Biotechnology, Seoul National University of Science and Technology, Seoul 01811, Republic of Korea, 2Interdisciplinary Program of Bioenergy and Biomaterials Graduate School, College of Engineering, Chonnam National University, Gwangju 61186, Republic of Korea

PBM-100 Trait and Fertility Analysis of F1 Hybrid between Genetically Modified Brassica napus and B. rapa

Soo-In Sohn1*, Yoonsung Cho1, Hyeong Joong Kang1, Young Ju Oh2 1Biosafety Division, National Institute of Agricultural Sciences, 2Institute for Future Environmental Ecology, Institute for Future Environmental Ecology

PBM-101 The interaction of polystyrene nanoplastics with albumin by size and thermodynamic analysis

Jina Park, Eunji Oh, Moonsung Choi*


PBM-102 Identification and characterization of OsWRKY transcription factor in the leaf architecture of rice (Oryza sativa L.)

Songhwa Chae1, Joung Sug Kim1, Kyong Mi Jun2, Yoonmok Pahk2, Duk-Ju Hwang3,

Gang-Seob Lee3, Baek Hie Nahm1,2, Yeon-Ki Kim1* 1Division of Bioscience and Bioinformatics, Myongji University, 2Genomics Genetics Institute, GreenGene Biotech Inc., 3Department of Agricultural Biotechnology, National Institute of Agricultural Science

42



PNB-1 Anti-inflammatory potential of an ethanolic extract of Sargassum serratifolium in nasal polyp-derived fibroblasts

Mijin Yim*

Department of Applied Research, National Marine Biodiversity institute of Korea

PNB-2 Molecular basis involved in the blocking effect of antidepressant metergoline on C-type inactivation of Kv1.4 channel



PNB-3 Effects of Alisma Rhizome on ligand-gated ion channels activity Jaeeun Lee, Junho Lee*


PNB-4 Plasma Treated Dimerization of (+)-Catechin with α-Glucosidase Inhibitory EffectJeong Hun Kim, Gyeong Han Jeong, Jehyeon Jo, Tae Hoon Kim*

Department of Food Science and Biotechnology, Daegu University, Gyeongsan 38453, Republic of Korea

PNB-5 Studies on Chemical Constituents from Gnetum macrostachyum Changon Seo1, Won Sik Jeong1, Jin Gwan Kwon1, Yeon Wo Jung1, Jung A Lee1, Eun-Kyung Ahn1,

Jae-Shin Kang2, Woo Hee Kim2, Jin Kyu Kim1, Chun Whan Choi1, Joa Sub Oh3, Seong Su Hong1* 1Bio-Center, Gyeonggido Business & Science Accelerator (GBSA), 2Biological Genetic Resources Utilization Division, National Institute of Biological Resources (NIBR), 3College of Pharmacy, Dankook University

PNB-6 Combrequadranols A and B, two new cycloartane triterpenes from the stems of Combretum quadrangulare Changon Seo1, Ji Eun Lee1, Yun-Hyeok Choi1, Jae-Shin Kang2, Chang Woo Hyun2, Jung A Lee1,

Eun-Kyung Ahn1, Jin Gwan Kwon1, Jin Kyu Kim1, Chun Whan Choi1, Joa Sub Oh3, Seong Su Hong1* 1Bio-Center, Gyeonggido Business & Science Accelerator (GBSA), 2Biological Genetic Resources Utilization Division, National Institute of Biological Resources (NIBR), 3College of Pharmacy, Dankook University

43


PNB-7 Beneficial Effects of Safflower (Carthamus tinctorius L.) Seed on Scopolamine-Induced Memory Impairment in Mice

Chan Hum Park1, Min Jo Kim1, Chang Yeol Yang1, Ji Hyun Kim2, Meitong He2, Eun Ju Cho2,

Takako Yokozawa3, Yu Su Shin1* 1Department of Medicinal Crop Research, NIHHS, RDA, Eumseong 27709, Republic of Korea, 2Department of Food Science and Nutrition & Kimchi Research Institute, Pusan National University, Busan 46241, Republic of Korea, 3Graduate School of Science and Engineering for Research, University of Toyama, Toyama 930-8555, Japan

PNB-8 Antifungal Potential of Extracts from Different Parts of Mulberry Plant O Chul Kwon*, Wan Taek Ju, Hyun Bok Kim, Ji Hae Lee


PNB-9 Acaricidal Properties of (1S,4R,5R)-4-Methyl-1-(propan-2-yl)bicyclo[3.1.0]hexan-3-one and Its Structural Derivatives against Mites

Hui-Ju Kim1, Seon-A Choi1, Jeong-Bin Lee2, Min-Seung Kang1, Hoi-Seon Lee1* 1Department of Bioenvironmental Chemistry, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju 54896, Korea, 2Department of Landscape Architecture, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju 54896, Korea

PNB-10 Biological Properties of 2,2,4,4-Tetramethyl-6-(3-methylbutanoyl)cyclohexane-1,3,5-trione and Structure-Activity Relationships of Its Derivatives

Hui-Ju Kim, Ah-Hyeon Jeong, Seon-A Choi, Hoi-Seon Lee*

Department of Bioenvironmental Chemistry, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju 54896, Korea

PNB-11 Insecticidal Properties of 1,4-Naphthalenedione Isolated from Cercis chinensis Heartwood and Its Structurally Related Derivatives

Ah-Hyeon Jeong, Hui-Ju Kim, Hoi-Seon Lee*


PNB-12 Geranialdehyde and Its Structural Derivatives Show Acaricidal Properties against Dermatophagoides spp. and Haemaphysalis longicornisAh-Hyeon Jeong1, Jun-Hwan Park1, Hui-Ju Kim1, Hoi-Seon Lee1*, Jeong-Moon Kim2* 1Department of Bioenvironmental Chemistry, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju 54896, Korea, 2Department of Landscape Architecture, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju 54896, Korea

44


PNB-13 Repellent Activities of Litsea cubeba (Lour.) Fruit Oil and Its Major Components against Haemaphysalis longicornis (Acari: Ixodidae)

Hui-Ju Kim, Ah-Hyeon Jeong, Jun-Hwan Park, Hoi-Seon Lee*


PNB-14 Acaricidal Properties of Alpinia galanga (L.) Rhizome Oil and Its Major Components against Haemaphysalis longicornis (Acari: Ixodidae)

Hui-Ju Kim1, Min-Seung Kang1, Ah-Hyeon Jeong1, Jeong-Monn Kim2, Hoi-Seon Lee1* 1Department of Bioenvironmental Chemistry, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju 54896, Korea, 2Department of Landscape Architecture, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju 54896, Korea

PNB-15 Dual Properties of 5-Methylfurfural Derived from Valeriana fauriei and Its Structural Derivatives against Dermatophagoides spp., Tyrophagus putrescentiae and Haemaphysalis longicornisAh-Hyeon Jeong, Hui-Ju Kim, Seon-A Choi, Hoi-Seon Lee*


PNB-16 Antioxidant phytochemicals from Ikonnikovia kaufmanniana and their protection of DNA damage

Kamila Zhumanova, Aizhamal Baiseitova, Jeong Yoon Kim, Ki Hun Park*

Division of Applied Life Science (BK21 plus), IALS, Gyeongsang National University, Jinju 52828, Republic of Korea

PNB-17 New polyprenylated acylophoroglucinol from the flowers of Hypericum ascyron Abdul Bari Shah, Yeong Jun Ban, Zuo Peng Li, Ki Hun Park*


PNB-18 Caged Xanthones from Garcinia hanburyi inhibit Human Neutrophil ElastaseDa Hyun Jang, Jeong Ho Kim, Jeong Yoon Kim, Ki Hun Park*


45


PNB-19 6-Metoxyflavonols from the aerial parts of Tetragonia tetragonioides (Pall.) Kuntze and their anti-inflammatory activity

Yeong-Geun Lee1, Jin Ah Ryuk2, Joo Tae Hwang2, Hyoung-Geun Kim1, Youn-Hyung Lee3,

Dong Sung Lee4, Byoung-Seob Ko2, Nam-In Baek1* 1Graduate School of Biotechnology and Department of Oriental Medicine Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea, 2Korea Institute of Oriental Medicine, Daejeon 34054, Republic of Korea, 3Department of Horticultural Biotechnology of College of Life Sciences, Kyung Hee University, Yongin 17104, Republic of Korea, 4College of Pharmacy, Chosun University, Gwangju 61452, Republic of Korea

PNB-20 Diarylheptanoid derivatives from the Zingiber officinale rhizomes and their anti-oxidant activity

Yeong-Geun Lee1, Hyoung-Geun Kim1, Youn-Hyung Lee2, Se Chan Kang1, Tong Ho Kang1,

Nam-In Baek1* 1Graduate School of Biotechnology and Department of Oriental Medicine Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea, 2Department of Horticultural Biotechnology of College of Life Sciences, Kyung Hee University, Yongin 17104, Republic of Korea

PNB-21 New phenolics and triterpenoids from Lithocarpus mekongensis and their antioxidant activity and protective effects on auditory hair cell damaged by neomycin in zebrafish (Danio rerio) larvae

Hyoung-Geun Kim1, Youn Hee Nam1, Sun-Woo Joo1, Young Sung Jung2, Yeong-Geun Lee1,

Dae-Ok Kim2, Youn-Hyung Lee3, Dae Young Lee4, Nam-In Baek1* 1Graduate School of Biotechnology & Department of Oriental Medicine Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea, 2Department of Food Science and Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea, 3Graduate School of Biotechnology & Department of Horticultural Biotechnology, Kyung Hee University, Yongin 17104, Korea, 4Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, Republic of Korea

PNB-22 New isocoumarins and flavonoids from the fruits of Citrus grandis Osbeck and their antioxidant activity and protective effects on pancreatic islet damaged by alloxan in zebrafish (Danio rerio) larvae

Hyoung-Geun Kim1, Yoon Hee Nam1, Hyun-Ji Oh1, Joo Tae Hwang2, Yeong-Geun Lee1,

Tong Ho Kang1, Youn-Hyung Lee3, Dae Young Lee4, Byoung Seob Ko2, Nam-In Baek1* 1Graduate School of Biotechnology & Department of Oriental Medicine Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea, 2Korea Institute of Oriental Medicine, Daejeon 34054, Republic of Korea, 3Graduate School of Biotechnology & Department of Horticultural Biotechnology, Kyung Hee University, Yongin 17104, Korea, 4Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, Republic of Korea

46


PNB-23 Cold Atmospheric Plasma induces Immunogenic Cell Death in Cancer Cells Yongdae Yoon, Seung Joon Beak*

Seoul National University, College of Veterinary Medicine

PNB-24 Enhanced Protein Self Assembly through Novel Water Soluble Nitrilotriacetic Acid Ligands

Hee-Kwon Kim*

Department of Nuclear Medicine, Chonbuk National University

PNB-25 Development of Novel GUL-based Multivalent Inhibitors for Increased Affinity of Prostate Cancer

Young-Do Kwon, Hee-Kwon Kim*


PNB-26 A Molecular Basis For the Inhibition of Transient Receptor Potential Vanilloid Type 1 by Gomisin A

Chaelin Kim, Junho Lee*


PNB-27 The regulatory effect of triterpenoids on α3β4 nicotinic acetylcholine receptor activityShinhui Lee, Junho Lee*


PNB-28 Effects of triterpenoid Alisol-F on human 5-hydroxytryptamine 3A and α3β4 nicotinic acetylcholine receptor channel activity

Jaeeun Lee, Junho Lee*


PNB-29 Cosmetic Hydrogel Maskpacks with CelluloseHong-Joo Byun, Hyang-Yeol Lee*

Department of Biotechnology, Korea National University of Transportation

PNB-30 Cisplatin(IV) Complexes with Short Fatty AcidsHyang-Yeol Lee*


47


PNB-31 Induction of Cancer Cell-Selective Apoptosis by Autocrine Motility FactorHee Sung Park*

Dept. Biotechnology, Daegu Catholic University

PNB-32 Improvement of identification assay and HPLC-DAD method for the simultaneous determination of three marker compounds in Jaeumganghwatang extract granules and soft∙dried extract

Ka-Yeon Baek1, Hwa-Dong Lee2* 1Department of Korean Medicine Development, Korean Medicine R&D Team 2, 2Department of Korean Medicine Development, Strategic Planning Team

PNB-33 Effect of Different Plant Growth Regulators (PGRs) on Propagation from Multiple Egg-Shape Micro-Tubers (MEM) Culture of Pinellia Ternata (Thunb.) Breit. Tuber

Yong-Goo Kim1, Richard Komakech1,2, Youngmin Kang1,2* 1Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, 111 Geonjae-ro, Naju-si, Jeollanam-do 58245, Republic of Korea, 2University of Science & Technology (UST), Korea Institute of Oriental Medicine Campus, Korean Medicine Life Science Major, Daejeon 34054, Republic of Korea

PNB-34 A review on the botanical aspects, phytochemical contents, and pharmacological activities of Warburgia ugandensis

Denis Okello1,2,3, Richard Komakech1,3,4, Motlalepula Gilbert Matsabisa5, Youngmin Kang1,3* 1Korean Convergence Medicine Major, University of Science and Technology (UST), Republic of Korea, 2Gombe Secondary School, P. O. Box, 192, Butambala/Mpigi, Uganda, East Africa, 3Herbal Medicine Research Division, Korea Institute of Oriental Medicine (KIOM), Yuseongdae-ro, Yuseong-gu, Daejeon 34054, Republic of Korea, 4Natural Chemotherapeutics Research Institute, Ministry of Health, Kampala, Uganda, 5Department of Pharmacology, Faculty of Health Sciences University of Free State, Bloemfontein 9300, South Africa

PNB-35 Secondary Metabolites Production Based on Adventitious Root Cultures in Medicinal Plants

Endang Rahmat1,2, Youngmin Kang1,2* 1University of Science & Technology (UST), Korea Institute of Oriental Medicine, Korean Convergence Medicine major, Daejeon 34054, Republic of Korea, 2Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, 111Geonjae-ro, Naju-si, Jeollanam-do 58245, Republic of Korea

PNB-36 Effects of a combination of herbal extracts on non-alcoholic fatty liver disease (NAFLD) in mice fed a high-fat diet

Doo Jin Choi, Bo-Ram Choi, Dae Young Lee, Young-Seob Lee, Geum Soog Kim*

Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA

48


PNB-37 DYT-BE Suppress Proliferation and COX-2 Expression through the AKT pathway in HT-29

Ji-Eun Park, Eun-Kyung Son, Bo-Mi Kim, Jai-Hyun So, Hyo-Hyun Park*

Department of Korean Medicine Development, National Development Institute of Korean Medicine

PNB-38 Inhibitory effect of the halophyte Ligustrum japonicum on matrix metalloproteinases-2 and 9 in HT-1080 cells

Junse Kim1, Minjeong Park2, Youngwan Seo2* 1Ocean Science & Technology School, Korea Maritime & Ocean University, 2Division of Marine Bioscience, Korea Maritime & Ocean University

PNB-39 Inhibitory capability of Artemisia princeps on matrix metalloproteinases-2 and -9 in HT-1080 cells

Minjeong Park1, Junse Kim2, Yeongwan Seo1* 1Division of Marine Bioscience, Korea Maritime & Ocean University, 2Ocean Science & Technology School, Korea Maritime & Ocean University

PNB-40 Hepatoprotective Effect of Platycodon grandiflorum Extract on Alcohol-Induced Liver Damage in Mice and HepG2 Cells

Jeon Hwang-Bo1, Dahye Yoon2, Seon Min Oh2, Bo-Ram Choi2, Youn-Hyung Lee3, Dae Young Lee2* 1Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea, 2Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, Republic of Korea, 3Department of Horticultural Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea

PNB-41 The Inhibitory Effects of Manassantin B on VEGF-A-induced Lymphangiogenesis and Lymph Node Metastasis in an Oral Sentinel Lymph Node Animal Model

Jeon Hwang-Bo, Mun Gyeong Bae, In Sik Chung*

Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea

PNB-42 Development and validation of a HPLC-DAD method for ethanolic extracts of Lespedeza bicolor roots

Hyun Sim Woo1,2, Yu Jin Oh1, Hae Jin Cho1, Min-Sung Lee1, Yeong-Su Kim1*, Dae Wook Kim1* 1Department of Forest Plant Industry, Baekdudaegan National Arboretum, 2Division of applied life Science, Institute of Agriculture and Life Science, Gyeongsang National University

49


PNB-43 Evaluation of antioxidant and antibacterial activities of Lamium album L. var. barbatumHae Jin Cho1,2, Yu-Jin Oh1, Hyun Sim Woo1, Yeong-Su Kim1*, Dae Wook Kim1* 1Forest Plant Industry Department, Baekdudaegan National Arboretum, 2School of Biological Sciences and Institute of Microbiology, Seoul National University

PNB-44 Antibacterial and Antioxidant activities of Parthenocissus tricuspidata extracts Yu-Jin Oh1,2, Hae Jin Cho1, Hyun Sim Woo1, Yeong-Su Kim1*, Dae Wook Kim1* 1Forest Plant Industry Department, Baekdudaegan National Arboretum, 2Advanced Bio and Fermentation Convergence, BK21 Plus, Kookmin University

PNB-45 Cultivated Wild Ginseng Attenuates Angiogenesis by Inhibiting the Phosphorylation of FAK and Src

Jin Soo Kim1, Jungeun Kim1, Jong Eel Park1, Dong Hee Kim2, Young Mi Seok1, Hyo Jung Kim1* 1Department of Korean Medicine Development Korean Medicine R&D Team 1, National Development Institute of Korean Medicine, Kyeongsan 38540, Republic of Korea, 2Department of Industrialization Guidance Team, National Development Institute of Korean Medicine, Kyeongsan 38540, Republic of Korea

PNB-46 Nematicidal Activity of Benzyloxyalcohol against Bursaphelenchus xylophilus Junheon Kim1*, Jun Oh Park1, Su Jin Lee1,2, Mannyun Kim1 1Forest Insect Pests and Diseases, National Institute of Forest Science, 2Department of Life Sciences, Incheon National University

PNB-47 Antioxidant and Anti-inflammatory effects of Fermented Samsoeum by Bioconversion Min A Kim, Han Saem Lee, Yu Mi Lim, So Hyun Chon, Eun Kyung Son, Jai Hyun So*

New Korean Medicine Team, National Development Institute of Korean Medicine

PNB-48 Preventive effect of immmature Citrus unshiu extract on o-toluidine and 2-ethylaniline-induced urinary bladder toxicity in Balb/c mice

Ji Hye Kim, Min Young Kim*


PNB-49 Skin aging effect of Umbilical cord blood-derived Stem Cell P on human fibroblast Jun-Seok Baek1*, Jua Kim1, Su-Young Kim1, Seung Hye Kang2, Se-Eun Jin2, Eunkyung Hong2,

Jeong-Kyun Yoo1, Chae Yeon Kim1, Young Soo Kim2* 1Material Development Team, Materials Science Research Institute LABIO Co., Ltd., 2Materials Science Research Institute, Materials Science Research Institute LABIO Co., Ltd.

50


PNB-50 Anti-inflammatory effect of solvent fractions in the aerial part and bulb of Lilium hansonii leichtlin

Hyun Jeong Kim, Su-Yeon Cho, In Guk Hwang, Byung Soon Hwang, Jeong-Sook Choe,

Hwan-Hee Jang*

National Institute of Agriculural Science, Rural Development Administration (RDA)

PNB-51 Delphinidin enhance radio-therapeutic effect via autophagy induction and JNK/MAPK pathway activation in non-small cell lung cancer

Seong Hee Kang1, Dong Ho Bak1, Yong Dae Park1, Byung Yeoup Chung1, Hyoung Woo Bai1,2* 1Research Division for Biotechnology, Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI), 2Radiation Biotechnology and Applied Radioisotope Science, University of Science and Technology (UST)

PNB-52 The hair growth promotion effects of Centipedegrass extract in vitro and in vivoFatuma Jumapili Ramadhani1,2, Seong Hee Kang1, Dong Ho Bak1, Sang Hun Park1,

Hyoung Woo Bai1,2* 1Research division for Biotechnology, Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI), 2Radiation Biotechnology and Applied Radioisotope Science, University of Science and Technology (UST)

PNB-53 Antimicrobial activities of an oxygenated cyclohexanone derivative isolated from Amphirosellinia nigrospora JS-1675 against various plant pathogenic bacteria and fungi

Hoa Thi Nguyen1, Soon Ok Kim2, Nan Hee Yu1, Hyeokjun Yoon2, Chang-Hwan Bae2, Joo Hong Yeo2,

Khanh Duy Le1, Van Thi Nguyen1, Jongmoon Kim1, Ae Ran Park1, Jin-Cheol Kim1* 1Department of Agricultural Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of Korea, 2Biological and Genetic Resources Assessment Division, National Institute of Biological Resources, 42 Hwangyeong-ro, Seo-gu, Incheon 22689, Republic of Korea

PNB-54 Biological control of various bacterial diseases by Bacillus methylotrophicus DR-08 producing oxydifficidin and difficidin

Seong Mi Im1, Nan Hee Yu1, Hee Won Joen1, Soon Ok Kim2, Hae Woong Park3, Ae Ran Park1,

Jin-Cheol Kim1* 1Department of Agricultural Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of Korea, 2Biological and Genetic Resources Assessment Division, National Institute of Biological Resources, 42 Hwangyeong-ro, Seo-gu, Incheon 22689, Republic of Korea, 3R&D Division, World Institute of Kimchi, 86 Kimchi-ro, Nam-gu, Gwangju 61755, Republic of Korea

51


PNB-55 Design, synthesis, and biological evaluation of 1-aryl-5-(2-(styryl)phenyl)penta-1,4-dien-3-one

Seunghyun Ahn1, Miri Yoo1, Jihyun Park2, Youngshim Lee2, Hyeok Lee2, Kyungrai Kang2,

Dongsoo Koh1, Soon Young Shin3, Young Han Lee3, Yoongho Lim2* 1Department of Applied Chemistry, Dongduk Women’s University, Seoul 02748, Korea, 2Division of Bioscience and Biotechnology, BMIC, Konkuk University, Seoul 05029, Korea, 3Department of Biological Science, Konkuk University, Seoul 05029, Korea

PNB-56 Phytochemical constituents of the whole plants of Aster koraiensis Ji-Young Kim1, Ranhee Kim1, Yutong Qi1, So-Ri Son2, Keon-Tae Park1, Dae Sik Jang1* 1Department of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Korea, 2Department of Biomedical Science and Technology, Graduate School, Kyung Hee University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Korea

PNB-57 Neuroprotective effect of Myristargenol A on the apoptosis of glutamate-induced mouse hippocampal HT22 cells

Jung Soo Park, Ki-Young Kim*

Kyung Hee University

PNB-58 Acyclic Triterpenoids from Alpinia katsumadai Inhibit IL-6-Induced STAT3 Activation Ye-Ji Park, Yu-Mi Yang, Seung Woong Lee, Mun-Chual Rho*

Immunoregulatory Material Research Center, Korea Research Institute of Bioscience and Biotechnology

PNB-59 Synthesis of thienopyrimidine derivatives as inhibitors of STAT3 activation induced by IL-6

Eun-Jae Park, Hee Ju Lim, Seung Woong Lee, Mun-Chual Rho*


PNB-60 Design and Preparation of Novel Oseltamivir Derivatives as Potential Therapy Agents Against Influenza Virus

Hee-Kwon Kim*


PNB-61 Validation of Analytical Method and Antioxidant Activities of Eriobotrya japonica Lindl. Leaves Extract according to Extraction Solvent

Na Young Byun, Hyun Hee Kim, Ji Hye Kim, Jae Seung Han, Seo Wk Lee, Hyang Eun Kim,

Eun Ju Kim, Soon Ho Yim*

Pharmaceutical Engineering, Dongshin University

52


PNB-62 Validation for Simultaneous Determination of three marker components in the Eriobotrya japonica extract by HPLC-DAD

Ye-Seul Yong, Hyun-Hee Kim, Woo-Jin Jeon, Min-Ah Kang, In-Hwa Kim, Hee-Eun Chae,

Soon-Ho Yim*

Department of Pharmaceutical Engineering, Dongshin University

PNB-63 Correlation between Antioxidant and Antioxidant Activity in 3029Extraction Conditions of Eriobotrya japonica (Thunb.) Lindley leaf and flesh

Ho Young Seon, Soon Ho Yim*, Yong Dae Kim, Si Young Jung, Yu Min Choi, Hy Bin Shin


PNB-64 Molecular Target of Berchemia racemosa Siebold & Zucc. for Anti-cancer Activity in Human Colorectal Cancer: Cyclin D1 proteasomal Degradation

Da Som Kim*, Yeongyeong Kang, Hyun Ji Eo, Youngki Park, Gwang Hun Park

Forest Medicinal Resources Research Center, National Institute of Forest Science

PNB-65 Anticancer Activity of Extracts from Different Parts of Berchemia racemosa var. magna in Human Cancer Cells

Yeongyeong Kang*, Gwang Hun Park


PNB-66 Resveratrol conjugated with gold nanoparticle induces cell cycle arrest in MCF-7 cell line

Dong Gun Lee, Yong Hun Joo, Hyeri Lee, Jimin Lee, Sang Gyu Lee, Na-Hyun Lee,

Namhyun Chung*

Department of Biosystems Engineering, College of Life Sciences & Biotechnology, Korea University

PNB-67 Rice extracts exhibit positive effects on mitochondria function of ovary removed mouse in tissue specific manner

Da Young Kim1, Min-Young Um2, Soo Im Shin1* 1Interdisciplinary Program of Bioenergy and Biomaterials Graduate School, College of Engineering, Chonnam National University, Gwangju 61186, Republic of Korea, 2Division of Functional Food Research, Korea Food Research Institute 245, Nongsaengmyeong-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do 55365, Republic of Korea

53


PNB-68 Phenotypic and genetic analysis of a new mutant related to development of tomato trichome and plant growth

Won-Ki Hong1, Jae-In Chun1, Seong-Min Kim1, Jin-Ho Kang1,2* 1GSIAT, Graduate School of International Agricultural Technology and Crop Biotechnology, Pyeongchang 25354, Republic of Korea, 2GBST, Institute/GreenBio Science and Technology, Seoul National University, Pyeongchang 25354, Republic of Korea

PNB-69 Assessment of the aromatase inhibitory activity of Ma-Huang-Tang (MHT) and its active compounds

Dong Ho Jung, Byoung Seob Ko*, Joo Tae Hwang, Bo-Jeong Pyun, Song Yi Yu

Division of Herbal Medicine Research, Korea Institute of Oriental Medicine

PNB-70 Anti-obesity effect of Standardized Extract of Microalga Phaeodactylum tricornutum Containing Fucoxanthin

Songyi Koo1, Jae-In Um2, Sang Min Kim3* 1Natural Product Informatics Center, KIST Gangneung Institute of Natural Products, 2AlgaeTech, 3Smart Farm Research Center, KIST Gangneung Institute of Natural Products

PNB-71 Aldose Reductase Inhibition of the Methanolic Extracts of Exotic Plants Ju Sung Lee1, Dong Geon Woo2, Ga Eul Cho2, Dong-Gu Lee1, Sanghyun Lee1* 1Department of Plant Science and Technology, Chung-Ang University, Anseong 17546, Korea, 2Sejong Academy of Science and Arts, Sejong 30099, Korea

PNB-72 Stability of Oleic acid and Linoleic acid on organic inputs derived from Tea see oilJeewon Rhee, Song-Hee Ryu*, Hyoin Yoon, Geun-Hyoung Choi, Hyejin Yu, Hyo-Sub Lee, Sung-

Jin Lim, Sang-Won Bark

Chemical Safety Division, National Institute of Agricultural Sciences, RDA

PNB-73 Deposition of iron-based inorganics during Fenton-based lignin humifications and its effects on plant stimulations

Ho Young Yoon, Hae Jin Jeong, Jong-Rok Jeon*

Department of Agricultural Chemistry and Food Science & Technology, Gyeongsang National University

PNB-74 Highly Stable and Fine-Textured Hybrid Microspheres for Entrapment of Cosmetic Active Ingredients

Ee Taek Hwang*

Center for Convergence Bioceramic Materials, Korea Institute of Ceramic Engineering & Technology

54


PNB-75 Protein Inserted into Vesicle for Artificial Liposome Preparation Ee Taek Hwang*


PNB-76 Exploring Step-by-Step Assembly of Nanoparticle: Cytochrome Biohybrid Photoanodes Ee Taek Hwang*


PNB-77 UPLC-QTOF/MS and HR-MAS NMR-based metabolomics applied for the quality evaluation of four processed Panax ginseng products

Bo-Ram Choi, Dahye Yoon, Jae-Suk Ban, Ryong Gong, Young-Seob Lee, Geum-Soog Kim,

Dae Young Lee*

Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, Republic of Korea

PNB-78 Identification and quantitative analysis of metabolites from Scrophularia buergeriana and their hepatoprotective effects against HepG2 Cells

Seon Min Oh1,2, Hyeon Seon Na1, Jeon Hwang-bo2, Dahye Yoon1, Young-Seob Lee1,

Geum-Soog Kim1, Dae Young Lee1* 1Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, 2Graduate School of Biotechnology and Department of Oriental Medicine Biotechnology, Kyung Hee University

PNB-79 Identification of Glycosylated Flavonoids and Steroidal Saponins from Oat (Avena sativa. L) Seedlings and Their Osteoclast Differentiation Inhibition

Hye-Lim Shin1,2, Ki-Chang Jang1, Mi-Ja Lee1, Hyun-Young Kim1, Ji-Eun Ra1, Hyeon-Mi Ham1,

Woo-Duck Seo1* 1Division of Crop Foundation, National Institute of Crop Science, 2Department of Biological Sciences, Chonbuk National University

PNB-80 Isolation and Identification of Isoflavones and Soyasaponins in Germs of Korean Soybean (Glycine max L.) Cultivars by UHPLC-Orbitrap-MS

Kwang-Sik Lee1,2, Hyeon-Mi Ham1, Hyun-Young Kim1, Mi-Ja Lee1, Ki-Chang Jang1, Hye-Lim Shin1,

Ji-Eun Ra1, Woo-Duck Seo1* 1Division of Crop Foundation, National Institute of Crop Science, 2Department of Crop Science and Biotechnology, Dankook University

55


PNB-81 Anti-inflammatory flavonoids from root bark of Broussonetia papyrifera in LPS-stimulated Raw264.7 cells

Mi Hyeon Park, Hyung Won Ryu, Doo-Young Kim, Yang Hee Jo, Sei-Ryang Oh*

Natural Medicine Research Center, KRIBB

PNB-82 Flavonoids from stem of Maackia amurensisWon Jun Kim, Seong Mi Lee, Hyun-Jae Jang, Hyung Won Ryu, Sei-Ryang Oh*


PNB-83 Metabolic Profiling Combined with Chemometrics Provides Prediction Models of Antioxidant Properties in Nine Mentha Species

Seung-A Baek1, Yun Ji Park2, Yongsoo Choi3, Sang Un Park2, Jae Kwang Kim1* 1Division of Life Sciences and Convergence Research Center for Insect Vectors, Incheon National University, 2Department of Crop Science, Chungnam National University, 3Systems Biotechnology Research Center, Korea Institute of Science and Technology (KIST)

PNB-84 Lutonarin from barley seedlings inhibits inflammation through suppressing of Nuclear Factor-kB signaling pathway on LPS-stimulated RAW 264.7 macrophage cells

Woo Duck Seo*, Mi-Ja Lee, Ji Eun Ra, Hyun Young Kim, Hyeon Mi Ham, Ki Chang Jang

National Institute of Crop Science, Rural Development Administration (RDA)

PNB-85 Characterization of policosanols and quality changes related to the growth times from the seedlings of various Korean wheat cultivars and screening for their AMPK activation

Woo Duck Seo, Ji Eun Ra, Hye Lim Shin, Kwang Sik Lee, Ki Chang Jang*, Mi Ja Lee,

Hyun Young Kim, Hyeon Mi Ham

National Institute of Crop Science, Rural Development Administration

PNB-86 Air Drying Temperature effects on Physiological and Effective Components of Thuja orientalis L.

Kyeong Yeong Song1, Jin Hwan Seo1, Youngju Kim2, Kyung Dong Lee1* 1Oriental Medicine Materials, Dongshin University, Naju, Jeonnam 520-714, Korea, 2Ireh Hi-Tech Inc., Eunha-gil 93, Suncheon 540-742, Korea

PNB-87 Physiological Activity Changes of Eriobotrya japonica Extracts with Air Drying Temperature Levels

Kyeong Yeong Song1, Jin Hwan Seo1, Youngju Kim2, Kyung Dong Lee1* 1Oriental Medicine Materials, Dongshin University, Naju, Jeonnam 520-714, Korea, 2Ireh Hi-Tech Inc., Eunha-gil 93, Suncheon 540-742, Korea

56


PNB-88 Antimicrobial Photodynamic Treatment for Gram Negative Bacteria with Hypericin Seemi Tasnim Alam1,2, Kyungsu Kang1,2* 1Natural Products Informatics Research Center, KIST Gangneung Institute of Natural Products, 2Division of Bio-Medical Science & Technology, Korea University of Science and Technology (UST)

PNB-89 Longifolioside A inhibits inflammatory responses through blockade of NF-κB and AP-1 in lipopolysaccharide-stimulated THP-1 macrophages

Eun Sol Oh1,2, Su Ui Lee1, Hyung Won Ryu1, Hyunju Ro2, Sung Tae Hong3, Sei Ryang Oh1* 1Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Republic of Korea, 2Department of Biological Sciences, College of Bioscience and Biotechnology, Chungnam National University, Daejeon, Republic of Korea, 3Department of Anatomy & Cell Biology, Department of Medical Science, College of Medicine, Chungnam National University, Daejeon, Republic of Korea

PNB-90 The effects of extracts of Bok choy (Brassica rapa subsp. chinesis) on transcriptome sequencing in Drosophila melanogasterSoo Yeon Cho, Hyeon Jung Kim, Sung Hyun Lee, Kyung A Hwang, Jeong Sook Choe,

Hwan Hee Jang*

Functional Food Division, National Institute of Agricultural Sciences, Rural Development Administration

PNB-91 Antimicrobial and Anti-inflammatory Activity of the Extracts and Fraction from Boehmeria nivea var. nipononivea

Ji Young Kim*, Gi Soo Jung, Sun Hee Lee, Soo-Kyung Yang, Gwanpil Song, Li Yeon Kim

Jeju Biological Resource, Jeju Biological Resource Co., Ltd., Jeju 63242, Korea

PNB-92 Expectation of Unknown Secondary Metabolites of Carex bostrichostigma maxim using UPLC-Q-TOF/MS Analysis

Bongkyu Song1, Dae-Choul Kim2, Qingzhen Liu2, Hye Yoon Park3, Ga Ryun Kim3, Wan Teak Im1,2* 1Ginsenoside Research Team, AceEMzyme Co., Ltd., 2Department of Biotechnology, Hankyong National University, 3Biological and Genetic Resources Assessment Division, National Institute of Biological Resources

PNB-93 Expectation of Unknown Secondary Metabolites of Rosa wichuraiana Crép. ex Déségl. Crep. ex Franch. & Sav using UPLC-Q-TOF/MS Analysis

Bong Kyu Song1, Dae-Choul Kim2, Qingzhen Liu2, Hye Yoon Park3, Ga Ryun Kim3, Wan Teak Im1,2* 1Ginsenoside Research Team, AceEMzyme Co., Ltd., 2Department of Biotechnology, Hankyong National University, 3Biological and Genetic Resources Assessment Division, National Institute of Biological Resources

57


PNB-94 Anti-Inflammatory Effect of Broccoli Leaves in LPS-Activated Macrophage Cells Sook Jahr Park, Eesul An, Jong Rok Lee*

Department of Pharmaceutical Engineering, Daegu Haany University

PNB-95 Production of the Rare Ginsenosides Gypenoside LXXV, through the Combinative Use of Two Recombination Enzymes

Dae-Choul Kim1, Bongkyu Song2, Hye Yoon Park3, Youn Kyoung Son3, Sang Yong Kim4,

Ji-Hyang Wee4, Wan Teak Im1,2* 1Department of Biotechnology, Hankyong National University, 2Ginsenoside Research Team, AceEMzyme Co., Ltd., 3Biological and Genetic Resources Assessment Division, National Institute of Biological Resources, 4Department of Food Science & Bio Technology, Shinansan University

PNB-96 Antibacterial Effect of Conjugated Linoleic Acid on Anaerobic Microorganisms Sook Jahr Park, Eesul An, Jong Rok Lee*


PNB-97 Antiallergic Effects of Family Cyperaceae ExtractsHye Yoon Park1*, Gi-Hum Nam2, Sarah Lee3, Ga Ryun Kim1 1Biological and Genetic Resources Assessment Division, National Institute of Biological Resources, Incheon, Korea, 2Plant Resources Division, National Institute of Biological Resources, Incheon, Korea, 3Biomaterials Research Group, National Institute of Biological Resources, Incheon, Korea

PNB-98 Chemical constituents of the culture broth of Antrodia albida E-Eum Woo, Su Ha Lee, In-Kyoung Lee, Bong-Sik Yun*

Division of Biotechnology, Chonbuk National University

PNB-99 Chemical constituents of the culture broth of the fungus Panus rudis and their antioxidant activity

Lee Su Ha, E-Eum Woo, In-Kyoung Lee, Bong-Sik Yun*


PNB-100 Costunolide ameliorates gastric acid reflux-induced esophageal injury via regulating NF-κB signaling pathway

Li Nan, Byung Kil Choo*

Department of Crop Agriculture and Life Science, School of Special Crop, Chonbuk National University, Jeonju 54896, Republic of Korea

58


PNB-101 Diketopiperazines from the culture broth of the fungus Trametes palisotii E-Eum Woo, Lee Su Ha, In-Kyoung Lee, Bong-Sik Yun*


PNB-102 Anti-oxidant activities and anti-inflammatory effects of ethanol extracts from Anethum graveolens L. on LPS-induced RAW 264.7 cells



PNB-103 Anti-oxidant activities and anti-inflammatory effects of ethanol extracts from Coriandrum sativum L. on LPS-induced RAW 264.7 cells



PNB-104 Neuroprotective Ellagitannins against glutamate-induced apoptosis in HT22 cellsJi Hoon Song1, Ji Yun Baek2, Hyun Young Kim2, Dahae Lee3, Do Hwi Park1, Ki Sung Kang1* 1College of Korean Medicine, Gachon University, 2Department of Food Science, Gyeongnam National University of Science and Technology, 3School of Pharmacy, Sungkyunkwan University

PNB-105 Inhibitory Effect of Cultivated Wild Ginseng Extract on TNFα/IFNγ-induced inflammation of Human Keratinocyte cells

Dong Hee Kim1*, Jong Eel Park2, Jungeun Kim2, Jin Soo Kim2, Jun Ho Son2, Hyo Jung Kim2 1Department of Korean Medicine Development Industrialization Guidance Team, National Development Institute of Korean Medicine, Kyeongsan 38540, Republic of Korea, 2Department of Korean Medicine Development Korean Medicine R&D Team 1, National Development Institute of Korean Medicine, Kyeongsan 38540, Republic of Korea

PNB-106 New Compounds from the Aerial Parts of Agastache rugosa and Their Anti-inflammatory Activity

Young Hye Seo1,2, Shin-Young Kang3, Ji-Sun Shin3, Seung Mok Ryu1, Byeong Cheol Moon1,

Goya Choi1, Dongho Lee4, Kyung-Tae Lee3, Jun Lee1,2* 1Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, 2Convergence Research Center for Diagnosis, Treatment and Care System of Dementia, Korea Institute of Science and Technology, 3Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, 4Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University

59


PNB-107 Enhancement of resveratrol production in peanut sprouts by elicitation method Soyoung Kim1,2, Yu Jeong Jeong1, Suhyun Park1, Jae Cheol Jeong1, Bo-Keun Ha2,

Cha Young Kim1* 1Biological Resource Center, Korea Research Institute of Bioscience Biotechnology, 2Department of Plant Biotechnology, College of Agriculture & Life Sciences, Chonnam National University

PNB-108 Enhanced production of avenanthramide by elicitation method in oat sprouts Tae Hee Kim1,2, Yu Jeong Jeong1, Suhyun Park1, Soyoung Kim1, Sung-Chul Park1, Saet Buel Lee1,

Jang Won Pyun1, Jae Cheol Jeong1, Cha Young Kim1*, Kwang Yeol Yang2 1Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, 2Department of Plant Biotechnology, College of Agriculture and Life Sciences, Chonnam National University

PNB-109 Protective Role of Cirsium japonicum var. maackii from Amyloid Beta25-35-induced Cellular Damage in C6 Glial Cells

Min Jeong Kim1, Ji Hyun Kim1, Sanghyun Lee2, Hyun Young Kim3, Eun Ju Cho1* 1Department of Food Science and Nutrition & Kimchi Research Institute, Pusan National University, Busan 46241, Korea, 2Department of Plant Science and Technology, Chung-Ang University, Anseong 17546, Korea, 3Department of Food Science, Gyeongnam National University of Science and Technology, Jinju 52725, Korea

PNB-110 Combination of Carthamus tinctorius L. Seed and Taraxacum coreanum Protects from Cognitive Impairment under Aβ25-35-Induced Alzheimer’s Disease Model

Mei Tong He1, Ji Hyun Kim1, Chan Hum Park2, Yu Su Shin2, Eun Ju Cho1* 1Department of Food Science and Nutrition & Kimchi Research Institute, Pusan National University, Busan 46241, Republic of Korea, 2Department of Medicinal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong 369-873, Republic of Korea

PNB-111 The Effects of Paeoniflorin on Lipopolysaccharide-induced Neuroinflammation and Cognitive Impairment in Mice

Hui Wen Meng1, Ah Young Lee2, Eun Ju Cho1* 1Department of Food Science and Nutrition & Kimchi Research Institute, Pusan National University, Busan 46241, Republic of Korea, 2Department of Food Science, Gyeongnam National University of Science and Technology, Jinju 52725, Republic of Korea

60


PNB-112 Sargassum Fulvellum Inhibits Inflammation and Oxidative Damage in Gluten-stimulated HaCat cells

Eun-Ji Shin1, Min Ju Kim1,2, Eui Jeong Han2, Disanayaka Mudiyanselage Dinesh Madusanka2,

Mawalle Kankanamge Hasitha Madhawa Dias2, Ginnae Ahn2,3* 1Department of Research and Development, Nantapizza 5000, Damyang, Korea, 2Department of Food Technology and Nutrition, Chonnam National University, Korea, 3Department of Marine Bio-Food Sciences, Chonnam National University, Korea

PNB-113 Inhibitory effects of 3,4-dihydroxybenzaldehyde isolated from Polysiphonia morrowii on IgE-mediated allergic response in mast cell and passive cutaneous anaphylaxis animal mouse model

Eui Jeong Han1, Soo-Jin Heo2, Eun-A Kim2, Eun-Ji Shin3, Min Ju Kim1,3,

Disanayaka Mudiyanselage Dinesh Madusanka1, Mawalle Kankanamge Hasitha Madhawa Dias1,

Ginnae Ahn1,4* 1Department of Food Technology and Nutrition, Chonnam National University, Yeosu, Korea, 2Jeju International Marine Science Center for Research & Education, Korea Institute of Ocean Science & Technology (KIOST), Jeju, Korea, 3Department of Research and Development, Nantapizza 5000, Damyang, Korea, 4Department of Marine Bio-Food Sciences, Chonnam National University, Yeosu, Korea

PNB-114 Protective effects of Sargassum horneri in UVB-irradiated Human Keratinocyte Min Ju Kim1,2, Eui Jeong Han1, Eun-Ji Shin2, Disanayaka Mudiyanselage Dinesh Madusanka1,

Mawalle Kankanamge Hasitha Madhawa Dias1, Kyounghoon Lee3, Ginnae Ahn1,4* 1Department of Food Technology and Nutrition, Chonnam National University, Korea, 2Department of Research and Development, Nantapizza 5000, Damyang, Korea, 3Department of Marine Production Management, Chonnam National University, Korea, 4Department of Marine Bio-Food Sciences, Chonnam National University, Korea

PNB-115 Effect of Aster yomena (Kitam.) Honda against Obesity and Insulin Resistance in High Fat Diet-fed Mouse Model

Min Jeong Kim1, Jeong Sook Noh2, Sanghyun Lee3, Eun Ju Cho1, Hyun Young Kim4* 1Department of Food Science and Nutrition, Pusan National University, Busan 46241, Korea, 2Department of Food Science and Nutrition, Tongmyong University, Busan 48520, Korea, 3Department of Plant Science and Technology, Chung-Ang University, Anseong 17546, Korea, 4Department of Food Science, Gyeongnam National University of Science and Technology, Jinju 52725, Korea

PNB-116 Variation of Furanocoumarins in the Angelica dahurica Root Extracts due to the Different Source of Plant Materials and Extraction Methods Employed

Tan Steven Ryan Susanto, Jaehong Han*

Metalloenzyme Research Group, Department of Plant Science and Technology

61


PNB-117 Anti-Melanogenic Effect of Gedunin in B16F10 Melanoma Cell LineHwang-Ju Jeon, Yong-Chan Kim, Hye Seung Choe, Sung-Eun Lee*


PNB-118 Chemical constituents of the rhizome of Atractylodes macrocephala Jin Hong Guang1, Hwan Lee2, Hye Jin Kim1, Kwan-Woo Kim1, Dong-Sung Lee2, Dae Young Lee3,

Dahye Yoon3, Geum-Soog Kim3, Ren-Bo An4, Hyuncheol Oh1, Youn-Chul Kim1* 1College of Pharmacy, Wonkwang University, Iksan 54538, Republic of Korea, 2College of Pharmacy, Chosun University, 309 Pilmun-daero, Dong-gu, Gwangju 61452, Republic of Korea, 3Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, Republic of Korea, 4Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, (Yanbian University), Ministry of Education, Yanji, Jilin 133002, P.R. China

PNB-119 Alleviating effect of the Chrysanthemum zawadskii Herbich var. latilobum Kitamura extract via inhibiting prostaglandin F2α (PGF2α) on oxytocin-induced primary dysmenorrhea in mice

Ju Hong Kim1, Ji Hoon Lee1, In Chul Lee1, Young Bae Ryu1, Seong Hun Jeong1, Da Bin Jeon1,

Han Gyo Shin1, Ba Wool Lee1, Ji Hye Ha1, Kyoung Oh Cho2, Hyung Jun Kwon1* 1Functional Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology, 2Laboratory of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University

PNB-120 Effects of Heat-killed Enterococcus faecalis EF-2001 on Ethanol-induced Acute Gastric Mucosal Injury in Mice

Han Gyo Shin, Da Bin Jeon, Ba Wool Lee, Seong Hun Jeong, Ju Hong Kim, Ji Hye Ha,

Ji Young Park, Hyung Jun Kwon, Young Bae Ryu, In Chul Lee*

Functional Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology

PNB-121 Spiraea prunifolia var. simpliciflora attenuates inflammatory responses in a murine model of lipopolysaccharide-induced acute lung injury via suppression of MAPK and NF-κB

Ba Wool Lee, Han Gyo Shin, Seong Hun Jeong, Da Bin Jeon, Ju Hong Kim, Ji Hye Ha,

Ji Young Park, Hyung Jun Kwon, Young Bae Ryu, In Chul Lee*


PNB-122 Ameliorative effect of Ficus auriculata Lour. extract in testosterone-induced benign prostatic hyperplasia (BPH)

Jae In Lee, Jong Sub Lee, Min Ju Lim, Seung Bin Park, Dong Young Kim, Ji Hoon Lee,

Hyung Jun Kwon, In Chul Lee, Ji Young Park, Young Bae Ryu*


62


PNB-123 Inhibitory Effect of Callus Extract from Euphorbia maculate on Melanogenesis in B16F10 Cells

Jong Sub Lee, Min Ju Lim, Jae In Lee, Seung Bin Park, Dong Young Kim, Ji Hoon Lee,



PNB-124 Anti-melanogenic effect of callus extract from Pinellia ternata in α-MSH stimulated B16F10 Cells

Seung Bin Park, Jong Sub Lee, Min Ju Lim, Jae In Lee, Dong Young Kim, Ji Hoon Lee,



PNB-125 Effect of Sapium rotundifolium hemsl extract on testosterone-induced benign prostatic hyperplasia

Min Ju Lim, Hyung Jun Kwon, Jong Sub Lee, Jae In Lee, Young Bae Ryu, Ji Young Park*


PNB-126 Combination of GU-10 water extract and curcumin exhibits antiviral activity against porcine epidemic diarrhea virus infection in piglets

Da Bin Jeon1, In Chul Lee1, Seong Hun Jeong1, Ji Young Park1, Young Bae Ryu1, Joo Hong Kim1,

Han Gyo Shin1, Ba Wool Lee1, Ji Hye Ha1, Kyoung Oh Cho2, Woo Song Lee1, Hyung Jun Kwon1* 1Functional Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology, 2Laboratory of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University

PNB-127 Anti-allergic inflammatory effect of alendronate in mast cellsJihye Lee1, Eui Jeong Han2, Ginnae Ahn2, Kyungsook Jung1* 1Functional Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology, 2Department of Marine Bio-Food Sciences, Chonnam National University

PNB-128 C-phycocyanin containing multifunctional chitosan-coated poly(lactic-co-glycolic acid) nanoparticles to treat atopic dermatitis by spatiotemporally controlled codelivery of ceramide

Seul Ki Min1*, Hoo Cheol Lee2, Hwa Sung Shin2 1Division of Polar Life Science, Korea Polar Research Institute, 2Department of Biological Engineering, Inha University

63


PNB-129 Gyeonggi-do Beauty Resource Bank [Cosmetic resource DB construction project]Changon Seo, Jin Kyu Kim, Woo Jung Kim, Mi Suk Seo, Jung A Lee, Young Rak Cho,

Kyu Hee Park, Jong Suk Lee*

Bio-Center, Gyeonggido Business & Science Accelerator (GBSA)

PNB-130 Investigation of Drug-herbs and Anti-hypertensive Drugs in Human HepatocytesJin-Moo Lee1, Sun-Young Ahn1, Young-Hoon Kim1, Hye Jin Cha1, Jisoon Shin1, Hye Hyun Yoo2,

Soo Kyung Suh1*1Pharmacological Research Division, Toxicological Evaluation and Research Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, 187 Osong Saengmyeong 2-ro, Heungdeok-gu, Chungju-shi 28159, Republic of Korea, 2College of Pharmacy, Hanyang University, 55 Hanyang University-Ro, Sangnok-go, Ansan-si, Republic of Korea


PES-1 Land Suitability Assessment of Apples and Pears by Combining Classification Results by Climate and Soil Information in South Korea

Yong Seok Kim*, Kee Kyung Kang, Kyo Moon Shim, Ji Na Hur

Climate Change & Agroecology Division, National Institute of Agricultural Sciences

PES-2 Isolation and characterization of Pseudomonas geniculate ANG3, Exiguobacterium acetylicum ANG40 and Burkholderia stabilis ANG51 isolated from soil

Hee Sook Kim1, Ji-Youn Kim1, Song Min Lee1, Hye-Jung Park1, Sang-Hyeon Lee2, Jeong Su Jang1*,

Mun Hyon Lee1 1Food Research Center, Angel Co., Ltd., 2Major in Pharmaceutical Engineering, Division of Bioindustry, Silla University

PES-3 Molecular biological analysis of Bt-transgenic (Bt-9) rice and its effect on Daphnia magna feeding

Jiyoung Kim, Soo-Yun Park, Doh-Won Yun, Myung-Ho Lim, Ancheol Chang, Sung-Dug Oh*

Department of Agricultural Biotechnology, National Institute of Agricultural Sciences

PES-4 Influence of heading date difference on gene flow from PAC rice to rice cultivars Eunji Bae, Doh-Won Yun, Gang-Seob Lee, Ancheol Chang, Sung-Dug Oh*


64


PES-5 Catalytic Activity of Azo Dye Orange G in Heterogeneous Fenton Oxidation with Fe-Impregnated Biochar Catalyst

Jong-Hwan Park1, Seong-Heon Kim2, Dong-Cheol Seo1* 1Division of Applied Life Science (BK21 Plus) & Institute of Agriculture and Life Science, Gyeongsang National University, 2Soil and Fertilizer Division, National Institute of Agricultural Sciences

PES-6 Metabolic engineering of Clostridium acetobutylicum for the production of short-chain ester

Hyeon Jeong Seong, Hyeon Ji Noh, Yu-Sin Jang*

Institute of Agriculture & Life Science (IALS), Department of Agricultural Chemistry and Food Science Technology, Division of Applied Life Science (BK21 Plus Program), Gyeongsang National University, Jinju, Republic of Korea

PES-7 Optimization of residual analysis method for flutriafol in agricultural produce using the QuEChERS method with LC-MS/MS

Hyun Ho Noh1, Hyeyoung Kwon2*, Danbi Kim1, Chang Jo Kim1, Byeong-Chul Moon1, Jin-Ho Ro1,

Min-Seok Oh1, Hyo Sub Lee1, Sujin Baek1, Kee Sung Kyung3 1Chemical Safety Division, National Institute of Agricultural Sciences, 2Rural Development Administration, Planning and Coordination Bureau, 3Department of Environmental and Biological Chemistry, Chungbuk National University

PES-8 Effect of different types of UV radiation on Triton X-100 degradationDong Gon Yoo, Seong Ho Yun*, Eun Hea Jho*

Department of Environmental Science, Hankuk University of Foreign Studies

PES-9 Molecular and Functional study of Insect Odorant Receptor Yong Kun Shim, Je Jun Oh, Sanung Eom, Junho Lee*


PES-10 Effect of organic fertilizer on relative efficiency ratio of watermelon and chemical properties of plastic film house soils with different electrical conductivity

Myung Sook Kim*, Seong-Jin Park, Seong-Heon Kim, Hyun-Young Hwang

Soil and Fertilizer Division, National Institute of Agricultural Sciences

PES-11 Isolation and characterization for plant growth promoting bacteria from seaweed treated soil

Jeong Won Seo*, Yeon Jong Koo

Department of Agricultural Chemistry, Chonnam National University

65


PES-12 Uptake of veterinary antibiotics by plants grown in soil contaminated with veterinary antibiotics

Reep Pandi Tasho, Hui Soo Jun, Ye Ji Mun, Jae Young Cho*

Department of Bioenvironmental Chemistry, Chonbuk National University, Jeonju, Republic of Korea

PES-13 Observed mycorrhizal frequency of plants grown in soil treated with veterinary antibiotics



PES-14 Risk assessment of sulfadimethoxine, streptomycin, and oxytetracycline in three plant types based on germination index



PES-15 Activity of detoxification phase I and phase II enzymes in plants grown in veterinary antibiotics contaminated soil



PES-16 Effect of sulfadimethoxine, streptomycin and oxytetracycline on the tolerance index and free proline content of plants



PES-17 The fate of 15N-labelled Organic Materials applied to Chinese Cabbages Cropping System

Cho-Rong Lee*, Yura Oh, Nan-Hee An, Jung-Lai Cho, Sang-Min Lee

Organic Agriculture Division, Department of Agricultural Environment, National Institute of Agricultural Sciences, Rural Development Administration

PES-18 Effect of Controlled Release Fertilizers on Young Oil Palm Trees in Nursery Yong-Sang Lee1,2*, Hoon Kim3 1Life Science Institute, Enbio. Co., Ltd., 37-20, Beonyoungro-28beon gil, Gunpo-si 15880, Republic of Korea, 2Research and Development Division, PT. Tunas Sawa Erma, Jalan Garuda Spadem No. 2B, Merauke, Papua 99611, Indonesia, 3Resources Management Division, Korindo, Jalan MT. Haryono Kav. 62, Jakarta 12780, Indonesia

66


PES-19 Removal of Odorous Compounds from Livestock Facility using Alkaline Liquid Spray Saem-Ee Woo*, Gwanggon Jo, Si Young Seo, Taehwan Ha, Yu Na Jang, Min Woong Jung,

Jung-Hoon Kwag, Deug-Woo Han

Animal Environment Division, National Institute of Animal Science, RDA

PES-20 Correlation Analysis of Odor Indicators to estimate Odor Strength in Pig Farm Saem-Ee Woo*, Gwanggon Jo, Si Young Seo, Taehwan Ha, Yu Na Jang, Min Woong Jung,

Hung-Hoon Kwag, Deug-Woo Han


PES-21 Assesmment of surface ditch irrigation for upland-crops in a reclaimed tidelands Hui Soo Jun1, Jae Kwon Son2, Jae Do Song2, Jae Young Cho1* 1Department of Bioenvironmental Chemistry, Chonbuk National University, Jeonju, Republic of Korea, 2Department of Agricultural Engineering, Chonbuk National University, Jeonju, Republic of Korea

PES-22 Effects of open channel irrigation on upland-crops in a reclaimed land Hui Soo Jun1, Jae Kwon Son2, Jae Do Song2, Jae Young Cho1* 1Department of Bioenvironmental Chemistry, Chonbuk National University, Jeonju, Republic of Korea, 2Department of Agricultural Engineering, Chonbuk National University, Jeonju, Republic of Korea

PES-23 Improvement of soil physical properties in reclaimed land using soil conditioner Hui Soo Jun1, Jae Kwon Son2, Jae Do Song2, Jae Young Cho1* 1Department of Bioenvironmental Chemistry, Chonbuk National University, Jeonju, Republic of Korea, 2Department of Agricultural Engineering, Chonbuk National University, Jeonju, Republic of Korea

PES-24 Evaluation of Food Waste Compost on Crop Growth and Soil Properties Seong Heon Kim, Hyun Young Hwang, Myung Sook Kim, Seong Jin Park*

Division of Soil and Fertilizer, National Institute of Agricultural Sciences, Rural Development Administration

PES-25 Desalinization of salt-affected reclaimed land using detergent to substitute exchangeable cations

Hui Soo Jun1, Jae Kwon Son2, Jae Do Song2, Jae Young Cho1* 1Department of Bioenvironmental Chemistry, Chonbuk National University, Jeonju, Republic of Korea, 2Department of Agricultural Engineering, Chonbuk National University, Jeonju, Republic of Korea

67


PES-26 Assessment of water washing method for desalinization in reclaimed tideland Hui Soo Jun1, Jae Kwon Son2, Jae Do Song2, Jae Young Cho1* 1Department of Bioenvironmental Chemistry, Chonbuk National University, Jeonju, Republic of Korea, 2Department of Agricultural Engineering, Chonbuk National University, Jeonju, Republic of Korea

PES-27 Effect of biochar type on nitrous oxide emissions in upland soil Sun Il Lee*, Gun Yeob Kim, Eun Jung Choi, Jong Sik Lee

Department of Climate Change & Agroecology, National Institute of Agricultural Sciences

PES-28 Application of Biochar for the reduction of odor during the livestock manure composting process

Hui Soo Jun1, Tae Kwon Park2, Jae Young Cho1* 1Department of Bioenvironmental Chemistry, Chonbuk National University, Jeonju, Republic of Korea, 2Yougiind. Co., LTD

PES-29 The effects of soil-treated herbicides on Sticky chickweed (Cerastium glomeratum) Chang-Wook Park, Seok-Min Lee, Hye-Bin Park, Yu-Na Kim, Hee-Soon Park, Ko-Eun Lee,

Eun-Jung Park, In-Jung Lee*

Division of Plant Biosciences, Kyungpook National University, Daegu 41566, Korea

PES-30 Monitoring of veterinary pharmaceuticals, chemical deodorants, and disinfectant Hui Soo Jun, Ye Ji Mun, Jae Young Cho*


PES-31 Persistence, photodecomposition and decomposition mechanism of agricultural chemical residues in water

Hui Soo Jun, Ye Ji Mun, Jae Young Cho*


PES-32 Phytoremediation of hazardous chemical residues from animal burial sites Hui Soo Jun, Ye Ji Mun, Jae Young Cho*


68


PES-33 Development of QuEChERS based method for the simultaneous determination of four sulfonylurea herbicides in cabbage and spinach by liquid chromatography tandem-mass spectrometry

Mohammad Mydul Islam Abul Kasem1, Byeong-Chul Moon1, Jin-Ho Ro1, Danbi Kim1, Sujin Baek1,

Min-Seok Oh1, Hyeyoung Kwon2* 1Department of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration, 2Planning and Coordination Bureau, Rural Development Administration

PES-34 Control of root-knot nematodes by a mixture of maleic acid and copper sulfate Jehyeong Yeon1, Ae Ran Park1, Nan Hee Yu1, Cuong Viet Han1, Ho Myeong Kim2,

Hae Woong Park2, Jin-Cheol Kim1* 1Department of Agricultural Chemistry, Institute of Enviromentally Friendly Agriculture, College of Agriculture and Life Science, Chonnam National University, Gwangju 61186, Republic of Korea,2R&D Division, World Institute of Kimchi, Gwangju 61755, Republic of Korea

PES-35 Study on Residual Properties of Cyazofamid and Its Metabolite in Herbal Medicine Cinidium RhizomeDong Yeol Lee*, Dong Kyu Jeong, Won Min Jeong, Young Sook Kil, Seung Mi Sin, Young Min Goo,

Sang Gon Kim


PES-36 Residual Characteristics and Processing Factors of Cyantraniliprole and Its Metabolite in Cinidium Rhizome

Dong Kyu Jeong, Dong Yeol Lee*

Research and Development Team, Gyeongnam Oriental Anti-Aging Institute

PES-37 Aroclor Accelerates the Toxicity of POPs in Activity of Mitochondrial complex III from Tail of Zebrafish

Songhee Lee1, Eun Ko2, Sooim Shin1,2* 1Department of Bioengineering and Biotechnology, College of Engineering, Chonnam National University, Gwangju 61186, Republic of Korea, 2Interdisciplinary Program of Bioenergy and Biomaterials Graduate School (BK21 Plus Program), Department of Bioengineering and Biotechnology, Chonnam National University, Gwangju 61186, Republic of Korea

PES-38 Determination of analysis method for the pesticide residue of chlormequat with LC-MS/MS in paprika and potato

Sujin Baek1, Min-Seok Oh1*, Hyeyoung Kwon2, Hyun Ho Noh1, A. K. M. Mydul Islam1, Danbi Kim1,

Jin-Ho Ro1, Byeong-Chul Moon1 1Chemical Safety Division, National Institute of Agricultural Sciences, 2Planning and Coordination Bureau, Rural Development Agency

69


PES-39 Leaf chlorophyll measurement by SPAD meter for estimation of leaf nitrogen content in ‘Gamhong’/M.9 apple orchard

Seong Eun Lee*, In Bog Lee, Pyoung Ho Yi, Gopal Selvakumar

Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, RDA

PES-40 Risk Assessment of Operator Exposure During Treatment of Fungicide Fluazinam on Korean Cabbage Plantation

Il Kyu Cho*, Young Gon Oh, Dong-Gi Lee, Jae Ung Seol, Ji Myung Kim

Research Center for Environmentally Friendly Agricultural Life Sciences, Jeonnam Bioindustry Foundation

PES-41 Molecular responses to phosphine and ethyl formate in two developmental stages of two-spotted spider mite (Tetranychus urticae)

Kyeongnam Kim, Sung-Eun Lee*


PES-42 Novel resistance mechanisms in the rice weevil, Sitophilus oryzae to phosphineKyeongnam Kim, Hwang-Ju Jeon, Sung-Eun Lee*


PES-43 Residual Evaluation of Insecticide Bifenthrin Sprayed on Radishes (Raphanus sativus var. Sativus)

Young-Jin Hong1, Won-Bae Joh1, Seon-Yeong Im2, Ji-Hye Kim2, Byung-Hun Song2,

Dong-Jin Lee1,2* 1Department of Crop Science & Biotechnology, Dankook University, Cheonan-si 31116, Republic of Korea, 2DICA, Cheonan-si 31116, Republic of Korea

PES-44 Effects of Biochar and Effective Microorganisms on Ammonia Gas Emission from Upland Soil with Urea for Soybean Cultivation

Su-Lim Lee1, Jae-Hoon Lee1, Ju-Hyeon Lee1, Jong-Hwan Park2, Dong-Cheol Seo1,2* 1Department of Agricultural Chemistry and Food Science & Technology, Gyeongsang National University, 2Division of Applied Life Science (BK21 Plus) & Institute of Agriculture and Life Science, Gyeongsang National University

PES-45 Investigation of half-lives in soil and uptake by radish of veterinary antibiotics Song-Hee Ryu*, Hyoin Yoon, Jeewon Rhee, Hyejin Yu, Sung-Jin Lim, Geun-Hyoung Choi,

Hyo-Sub Lee, Sang-Won Bark


70


PES-46 Monitoring of paprika internal electrical conductivity under metal stress Seung Ju Cha1, Jin Hee Park1*, Seon Ju Kwon1, Joo Kyung Lee1, Hyun Jun Park2 1Chungbuk National University, Agricultural Chemistry, 2Soil Research Institute, Prumbio Co., Ltd.

PES-47 Effect of pyroligneous acid on amidase activity in soil Joo Kyung Lee1, Hyun Jun Park2, Seung Ju Cha1, Seon Ju Kwon1, Jin Hee Park1* 1Chungbuk National University, Agricultural Chemistry, 2Soil Research Institute, Prumbio Co., Ltd.

PES-48 Deposition and metal content of particulate matter on the surface of shrub and tree leaves

Seon Ju Kwon, Seung Ju Cha, Joo Kyung Lee, Jin Hee Park*

Chungbuk National University, Agricultural Chemistry

PES-49 Residual Patterns of Dithianon and Flufenoxuron in Lettuce as a Minor CropTae Hyun Lee, Kyu-Won Hwang, Jeong Hoon Sun, Hyun Jin Kim, Kyung Su Jung,

Joon-Kwan Moon*

Department of Plant Life and Environmental Sciences, Hankyong National University, Anseong 17579, Republic of Korea

PES-50 Spatial Distribution of Soil Chemical Properties Using GeostatisticsWoo Ri Go*, Yeon Kyu Sonn, Sang Ho Jeon, Byung Keun Hyun, Byung Hwan Seo, Sun Gang Yun

Soil and Fertilizer Division, National Institute of Agricultural Science, RDA

PES-51 Evaluation of Ammonium Ion Behavior for Obtaining Information on Secondary Particulate Matter Originating from Various Soils

Se-Wook Hwang1, Jong-Hwan Park1, Jeong-Woo Lee2, Dong-Cheol Seo1,2* 1Division of Applied Life Science (BK21 Plus) & Institute of Agriculture and Life Science, Gyeongsang National University, 2Department of Agricultural Chemistry and Food Science & Technology, Gyeongsang National University

PES-52 Determination of Optimal Conditions for the CO2 and NH3 Treatment for the Induction of the Infective Juveniles of Entomopathogenic Nematodes

Sang Heyong Park1, Kwang-Hyun Baek2*, Yeon Su Yu1* 1Research Division, Daedong Tech Co., Gyeongsan, Gyeongbuk 38401, Republic of Korea, 2Department of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 38541, Republic of Korea

71


PES-53 An abalone-derived bacteria has strong fucoidan metabolic activity and compete with plant to use soil nutrients

Yeji Gil, Heejeong Oh, So Hee Yang, Euyeon Kim, Yeonjong Koo*

Department of Agricultural & Biological Chemistry, Chonnam National University

PES-54 Chemical characteristics of groundwater based on different cropping types in Gyeongnam Province, South Korea

Jae Young Heo1*, Hyeon Ji Cho1, Young Han Lee1, Si Lim Choi1, So Jin Yeob2 1Environmental Agricultural Research Division, Gyeongsangnam-do Agricultural Research and Extension Services, 2Climate Change and Agroecology Division, National Institute of Agricultural Sciences

PES-55 Comparison of Cadmium Retain Capacities in Microplastic Polyethylene and Polypropylene Ju Hyun Eom1, Dong Cheol Seo1,2* 1Department of Agricultural Chemistry and Food Science & Technology, Gyeongsang National University, 2Division of Applied Life Science (BK21 Plus) & Institute of Agriculture and Life Science, Gyeongsang National University

PES-56 Carbon storage capacity of paddy soil with organic cultivation Yangsoo Han*, Choong-Bae Park, Hongshik Nam, Kwang-Lai Park, Kee-Choon Park

Organic Agriculture Division, Dept. of Agricultural Environment, NAAS, RDA

PES-57 Analysis of the distribution of assimilation products and the characteristics of transcriptomes in rice by submergence during the ripening stage

Hyeonseok Lee*

National Institute of Crop Science, Crop Production & Physiology

PES-58 Threshold concentration of arsenic in paddy soils causing changes in nutrient availability to rice plant

Myeong-Hui Kim, Bu-Ung Choi, Ji-Hyun Yoon, Oh-Do Kwon, Ji-Hyock Yoo*

Chemical Safety Division, National Institute of Agricultural Sciences

PES-59 Changes of Soil Organic Carbon stock and GHGs emission from LULUCF in South Korea (1990 to 2017)

Seong Jin Park*


72


PES-60 Non-negative matrix factorization for impurity profiling of seized methamphetamine Beom Jun Ko1, Dong Won Shin1, Jae Chul Cheong1, Wonho Lee2, Suhkmann Kim2,

Jin Young Kim1* 1Forensic Genetics & Chemistry Division, Supreme Prosecutors’ Office, Seoul 06590, Republic of Korea, 2Department of Chemistry, Center for Proteome Biophysics and Chemistry Institute for Functional Materials, Pusan National University, Busan 46241, Republic of Korea

PES-61 Long-term effects of soil amelioration methods on metal polluted farmlandsBuung Choi1, Ji-Hyock Yoo1*, Oh Do Kwon1, Jin Woong Park1, Ji Hyun Yoon1, Seon Mi Lee1,

Goo-Bok Jung2 1Chemical Safety Division, Department of Agro-Food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea, 2Soil and Fertilizer Division, Department of Agricultural Environment, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea

PES-62 Chemical properties of soils from ameliorated farmlands near closed metal mine Se In Kim, Ji Hyock Yoo*, Myung Hee Kim*, Seon Mi Lee*

Dep. of Agro-Food Safety, National Institute of Agricultural

PES-63 Arsenic Removal Utilizing Biogenic Lepidocrocite Reductively Formed by Clostridium Sp. Strain C8

Chang Hu Kang, Hor-Gil Hur*, Youri Yang, Jisu Lee, Yongseok Ko

School of Earth Sciences and Environmental Engineering, Gwangju Institute of Science and Technology (GIST), 123 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea

PES-64 Lipid profiles of Tetranychus urticae adults after exposure to ethyl formate and phosphine Kyeongnam Kim, Sung-Eun Lee*

School of Applied Bioscience, Kyungpook National University

PES-65 Monitoring of ambient ammonia in an agricultural area using diffusive passive air samplers

Ho-Young Lee1, Tien Do Van1, Min-Kyu Park1, Sang Ryong Lee2, Sung-Eun Lee3,

Sung-Deuk Choi1* 1School of Urban and Environmental Engineering, Ulsan National Institute of Science and Technology (UNIST), 2Department of Agricultural Convergence, Jeonju University, 3School of Applied Biosciences, Kyungpook National University

73


PES-66 Land-use Management for Sustainable Rice Production in Reclaimed Coastal Tideland Soils: A review

Hyun-Jin Park1, Hye-In Yang1, Se-In Park1, Bo-Seong Seo1, Dong-Hwan Lee1, Young-Jae Jeong1,

Ju-Yeon Lim1, Han-Yong Kim2, Woo-Jung Choi1* 1Department of Rural and Biosystems Engineering, Chonnam National University, 2Department of Applied Plant Science, Chonnam National University

PES-67 Assessment of Electrical Conductivity of Saturated Soil Paste from 1:5 Soil-Water Extracts for Reclaimed Tideland Soils in South-Western Coastal Area of Korea

Hyun-Jin Park1, Hye-In Yang1, Se-In Park1, Bo-Seong Seo1, Dong-Hwan Lee1, Young-Jae Jeong1,

Ju-Yeon Lim1, Han-Yong Kim2, Woo-Jung Choi1* 1Department of Rural and Biosystems Engineering, Chonnam National University, 2Department of Applied Plant Science, Chonnam National University

PES-68 Impact of different types of manure on the compost quality and greenhouse gas emissions during food waste composting

Hyun Young Hwang, Seong Jin Park*

Division of Soil and Fertilizer, National Institute of Agricultural Sciences

PES-69 Effect of soil amendments on As bioavailability in As-contaminated paddy soilDae-Won Kang1, Ji-Hyock Yoo2, Da-Young Kim3, Hyuk-Whan Song1, Oh-Kyung Kwon4,

Won-Il Kim4* 1Food Quality and Safety Center, Korea National Food Cluster, 2Chemical Safety Division, National Institute of Agricultural Science, 3Department of Environmental Horticulture, University of Seoul, 4O-Jeong Eco-Resilience Institute, Korea University

PES-70 Temperature response of soil organic matter decomposition in volcanic ash soils under agricultural land-use

Hye In Yang, Hyun-Jin Park, Se-In Park, Woo-Jung Choi*

Department of Rural & Bio-Systems Engineering, Chonnam National University, Gwangju 61186, Republic of Korea

PES-71 Applicability of circulating system of closed chamber and INNOVA for estimating the flux of ammonia emission from swine manure

Min-Suk Kim1,2, In-Sun Kang1, Sang-Ryong Lee1* 1Department of Agricultural Convergence, Jeonju University, 2O-Jeong Eco Resilience Institute, Korea University

74


PES-72 Vegetated ridge and sandbag may not reduce soil erosion and loss of carbon and nutrients from upland fields

Se-In Park1, Hye In Yang1, Hyun-Jin Park1, Bo-Sung Seo1, Dong-Hwan Lee1, Kwang-Sik Yoon1,

Han-Yong Kim2, Woo-Jung Choi1* 1Department of Rural and Biosystems Engineering, Chonnam National University, Gwangju 61186, Republic of Korea, 2Department of Applied Plant Science, Chonnam National University, Gwangju, 61186, Republic of Korea

PES-73 The performance of sediment trap for water pollutants and soil removal from cropland runoff

Han-Yong Kim1*, Woo-Jung Choi2, Hyeon-Jin Park2, Jae-Hyeok Park1 1Department of Applied Plant Science, Chonnam National University, 2Department of Rural and Biosystem Engineering, Chonnam National University

PES-74 A study of the interaction between benzalkonium chloride and albumin by spectroscopic methods

Sujin Park, Semi Kim, Moonsung Choi*


PES-75 Biotoxicity of UV photodegradation off-gas for 10 VOCs In Sun Kang1, Min Suk Kim1, Sang Ryong Lee1, Jinying Xi2* 1Department of Agricultural Convergence, Jeonju University, 2School of Environment, Tsinghua University

PFS Food Sciences

PFS-1 Survey on Harmful Materials of on Herbs Commonly Used for Food and Medicine in Busan

Nam Ho Kim*, Hye Kyung Cho, Jong Bae Youn, Seung Ju Lee

Drug Analysis Team, Busan Metropolitan City Institute of Health and Environment

PFS-2 Survey on Pesticide Residues and Heavy Metals of Local Agricultural Products in Busan Area

Ji-Yoon Lee1*, Kyeong-A Kim2, Dongu-Ju Park2, Sun-Mi Lee2, Han-Nui You2, Eun-Ju Song2,

Sang-Kee Min2 1Office of Eomgung Agricultural Products Inspection, Busan Metropolitan City Institute of Health and Environment, 2Office of Eomgung Agricultural Products Inspection, Busan Metropolitan City Institute of Health and Environment

75


PFS-3 A Study on Pesticide Residues Monitoring of Agricultural Products Used for Food and Medicine in Busan area

Younju Ok, Sohyun Park*

Office of Banyeo Agricultural Products Inspection, Busan Metropolitan City Institute of Health and Environment

PFS-4 A Study on the Content of Heavy Metals for Other Processed ProductsYon Koung Park*, Sung Ah Park, Yung Ran Na, Hyeon Jin Kim, Hyeon Jeong Kwon,

Hyun Nho Cho, Jung Mi Kang

Food Analysis Division, Busan Metropolitan City Institute of Health and Environment

PFS-5 Analysis of sodium diacetate in foods by HPLC-DAD Kun Ho An, Chan Lee, Ji Hyun Lee*

Department of Food Science and Technology, Chung-Ang University

PFS-6 Investigation of the quality changes for dried pepper powder after UV-C irradiation Ji-Yeon Bae1, Deuk-Yeong Lee1, Jin-Ho Rho2, Jin-Hyo Kim1* 1Department of Agricultural Chemistry, IALS, Gyeongsang National University, Jinju, 2Chemical Safety Division, National Institute of Agricultural Science, RDA, Wanju

PFS-7 Development of the Analysis and Content Evaluation Technology for Allergens in Rice Sang Gu Lee*, Si Myung Lee, Seon Woo Oh, Soo Yun Park, Tae Hoon Ryu, Hyeon Jung Kang

Biosafety Division, National Institute of Agricultural Science

PFS-8 Comparison of pesticide residue in Korean melon between sample preparation method in Korea and Japan

Danbi Kim, Jin-Ho Ro*


PFS-9 Application of Dispersive Liquid-liquid Microextraction for the Determination of Ochratoxin A in Grain Feeds

Mi Jeong Lee, Theresa Lee, Jeomsoon Kim, Ja Yeong Jang*

Microbial Safety Team, National Institute of Agricultural Sciences, Rural Development Administration

76


PFS-10 Occurrence of Fusarium Species and Mycotoxins in Unprocessed Cereals Mi Jeong Lee, Jung-Hye Choi, Ju-Young Nah, Hyun-Suk Jin, Soobin Yim, Theresa Lee,

Jeom-Soon Kim, Ja Yeong Jang*

Microbial Safety Team, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea

PFS-11 Application of Kinetic Models for Predicting the Degradation Rate of Triazole Pesticides in Perilla leaves

Hyo Jeong Kim1, Sang Hyeob Lee1*, Se Yeon Kwak1*, Aniruddha Sarker1*, Hye Rin Jeong1*,

Ae Ji Nam1*, Tae Hwa Kim2*, Jang Eok Kim1* 1School of Applied Biosciences, Kyungpook National University, 2Analysis Technology and Tomorrow, Daegu 41566, Korea

PFS-12 Cassia tora seed extract improves type 2 diabetic condition through the regulation of pancreatic mitochondrial enzymes’ activities and secretion of adiponectin

Eun Ko1, Min Young Um2, Moonsung Choi3, Sooim Shin1,4* 1Interdisciplinary Program of Bioenergy and Biomaterials Graduate School, College of Engineering, Chonnam National University, 2Division of Functional Food Research, Korea Food Research Institute, 3Department of Optometry, College of Energy and Biotechnology, Seoul National University of Science and Technology, 4Department of Biotechnology and Bioengineering, College of Engineering, Chonnam National University

PFS-13 Immature Citrus unshiu physiological activities during vinegar manufacturing: changes in polyphenolic contents and in vitro antioxidant activity

Hyeok Jun Yang, Min Young Kim*

Toxicology Lab, Major in Biomaterials, Jeju National University

PFS-14 Quality and bioactive properties of mulberry leaf according to blanching and hot-air dry conditions

Ji Hae Lee1, O-Chul Kwon1, Wan-Taek Ju1, Hyun-Bok Kim1, You-Young Jo1, Haeyong Kweon1,

Young Bo Lee2* 1Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea, 2Technology Services Team, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea

PFS-15 Profiling of Endogenous Metabolites in Multigrains Based on Metabolomics Hyejin Yu1,2, Jeonghak Lee2, Songhee Ryu1, Jeong-Han Kim2* 1Division of Chemical Safety, National Institute of Agricultural Sciences, 2Department of Agricultural Biotechnology, Seoul National University

77


PFS-16 Effect of selected PGPRs on the initial growth of wheat cultivar Sang Gyu Lee, Hyeri Lee, Dong Gun Lee, Jimin Lee, Na-Hyun Lee, Namhyun Chung*


PFS-17 Relevancy of nitrate reductase and leaf rust resistance in Korean wheat Hyeri Lee, Sang Gyu Lee, Dong Gun Lee, Jimin Lee, Minseo Kim, Namhyun Chung*


PFS-18 The relationship between rice yield and the occurrence of extreme weather events in South Korea

Jina Hur, Kyo-Moon Shim*, Yongseok Kim, Kee-Kyung Kang


PFS-19 Detection of genetically modified canola DP-073496-4 and MON88302 using event-specific multiplex PCR

Ji-Eun Park, Jin-Young Choi*, Hyun-Joong Kim*, Hae-Yeong Kim *

Department of Food Science and Biotechnology, Kyung Hee University

PFS-20 Real-time PCR based detection method for GM cotton and canola Min Ki Shin*

Food Safety Risk Assessment Division, National Institute of Food and Drug Safety Evaluation

PFS-21 Effect of Different Processing Methods on Quality and Functional Ingredients in Asparagus Juice Production

Thu Thi Nguyen, Chi Rac Hong, Munkhbat Saranchimeg, Sang Min Kim*

Smart Farm Research Center, KIST Gangneung Institute of Natural Products

PFS-22 Multiplex PCR assay for genetically modified cotton Jinyoung Choi, Jieun Park*, Hyunjoong Kim*, Haeyeong Kim*

Department of Food Science and Biotechnology, Graduate school of Biotechnology, Kyung Hee University

PFS-23 A Review on Processing Factors of pesticide Residues During Potato Processing Moo-Hyeog Im1*, Jeong Ah Kim1, Hye Su Lee1, Jeong-A Seo1, Seo Hong Kim2 1Food Engineering, Daegu University, 2Environmental and Biological Chemistry, ChungBuk National University

78


PFS-24 Effects of soybean varieties on quality characteristics of tofu Eunyeong Sim*, Hye Young Park, Areum Cheon, Mi-Jung Kim, Jieun Kwak, Yong Hee Jeon,

Choon Ki Lee

Department of Central Area Crop Science, National Institute of Crop Science, RDA

PFS-25 Multi-Analytical Method of Mycotoxin from Feed using LC-MS/MSHyeong Wook Jo1, Jeong Hoon Seon2, Kyu Won Hwang2, Joon Kwan Moon2* 1Hansalim Agro-Food Analysis Center, Hankyong National University Industry Academic Cooperation Foundation, 2Department of Plant Life and Environmental Sciences, Hankyong National University

PFS-26 Changes of phenolic composition and antioxidant activity of strawberries (cv. ‘Seolhyang’ and ‘Jukhyang’) during ripening

Dong Hee Ahn1, Jinwook Lee2*, Jihyun Lee1* 1Department of Food Science and Technology, Chung-Ang University, Anseong, Republic of Korea, 2Department of Plant Science and Technology, Chung-Ang University, Anseong, Republic of Korea

PFS-27 Change of the nutrient and phytochemical constituents and antioxidant and antiinflammatory effects from fresh to processed mountain-cultivated ginseng sprouts

Su Cheol Kim1, Yong Min Kang2, Jin Hwan Lee3, Kye Man Cho1,2* 1Department of Food Science, Gyeongnam National University of Science and Technology, 2Herbal Medicine Resources Research, Korea Institute of Oriental Medicine, 3Department of Life Resources Industry, Dong-A University

PFS-28 Change of nutrient, ginsenoside, and volatile flavor constituents and antioxidant activity during food processing of mountain-cultivated ginseng with the cocktail lactic acid bacteria

Su Cheol Kim1, Yong Min Kang2, Jin Hwan Lee3, Kye Man Cho1,2* 1Department of Food Science, Gyeongnam National University of Science and Technology, 2Herbal Medicine Resources Research, Korea Institute of Oriental Medicine, 3Department of Life Resources Industry, Dong-A University

PFS-29 Comparison in phytochemical constituents (ginsenosides, phenolic acids, and flavonols) of antioxidant activities in above and below grounds of ginseng sprouts for different roasting conditions

Ho Jeong Park1, Su Cheol Kim1, Du Young Cho1, Hee Sun Jung1, Jin Hwan Lee2, Kye Man Cho1,3* 1Department of Food Science, Gyeongnam National University of Science and Technology, 2Department of Life Resources Industry, Dong-A University, 3Herbal Medicine Resources Research, Korea Institute of Oriental Medicine

79


PFS-30 Change of nutritional constituents and in vitro antioxidant activity during the food processing of isoflavone-enriched soy leaves with the cocktail lactic acid bacteria

Du Young Cho1, Su Cheol Kim1, Ho Jeong Park1, Hee Sun Jung1, Sang Hee Nam1, Jin Hwan Lee2,

Kye Man Cho1,3* 1Department of Food Science, Gyeongnam National University of Science and Technology, 2Department of Life Resources Industry, Dong-A University, 3Herbal Medicine Resources Research, Korea Institute of Oriental Medicine

PFS-31 Change of γ-aminobutyric acid and isoflavone constituents and in vitro antioxidant activity during the lactic acid fermentation of isoflavone-enriched soy leaves

Hee Sun Jung1, Su Cheol Kim1, Ho Jeong Park1, Du Young Cho1, Sang Hee Nam1, Jin Hwan Lee2,

Kye Man Cho1,3* 1Department of Food Science, Gyeongnam National University of Science and Technology, 2Department of Life Resources Industry, Dong-A University, 3Herbal Medicine Resources Research, Korea Institute of Oriental Medicine

PFS-32 Development of Analytic Method for Maltitol in Mouthwash using High-Performance Liquid Chromatography coupled with Evaporative Light Scattering Detector

Sangseop Kim1, Kyoung-Moon Han1, Jihyun Shin1, Soae Ji1, Rheeda Lee1, Soojeong Son2,

Kyunghun Son1* 1Cosmetic Research Team, National Institute of Food and Drug Safety Evaluation, 2Pharmaceuticals and Medical Devices Research, National Institute of Food and Drug Safety Evaluation

PFS-33 GC/MS method development of ethyl carbamate analysis in various food matrices Seung Min Kim, Sung Hyeon Jung, Myung-Sub Chung, Jihyun Lee*

Department of Food Science and Technology, Chung-Ang University, Korea

PFS-34 Physicochemical analysis of Zanthoxylum schinifolium using HPLCA Yeong Lee1, Hyo Seon Kim1, Goya Choi1*, Chang-Seob Seo2* 1Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, 2Herbal Medicine Research Division, Korea Institute of Oriental Medicine

PFS-35 Chemical Properties Variation of Morus bombycis using Decompressed Heat Pump Dryer

Hee-Jung Park, Hyun-Soo Kim, Sang-Hyun Lee*

Department of Forest Environmental Science, Chonbuk National University

80


PFS-36 Residual characteristics of pyrifluquinazon and its main metabolite (pyrifluquinazone-1H) in minor crops

Jiho Lee*, Kwanghun Lee, Suyoung Ju, Jinchan Kim, Kyujin Jung, Sangchul Kim

Bio Technology Division, Korea Conformity Laboratories

PFS-37 Protective Role of Kimchi under Different Fermentation Stages against Amyloid Beta-Induced C6 Glial Cell Damage

Ji Myung Choi1, Eun Ok Choi1, Yung Hyun Choi2, Eun Ju Cho1* 1Department of Food Science and Nutrition, Kimchi Research Institute, Pusan National University, 2Department of Pathology and Research Institute of Korean Medicine, Anti-Aging Research Center, Dong-eui University

PFS-38 Protective Effect of Samultang and Its Four Herbal Plants from Reactive Oxygen Species under in Vitro and Cellular System

Yu Ri Kwon1, Mi Na Nam1, Ah Young Lee2, Seung Mi Sin3, Young Min Goo3, Eun Ju Cho1* 1Department of Food Science and Nutrition, Pusan National University, Busan 46241, Republic of Korea, 2Department of Food Science, Gyeongnam National University of Science and Technology, Jinju 52725, Republic of Korea, 3Gyeongnam Oriental Anti-aging Institute, Gyeongnam 52215, Republic of Korea

PFS-39 Simultaneous analysis of fungicide tecloftalam and its metabolites tecloftalam imide in rice using LC-MS/MS

Junghak Lee, Jong Hwa Lee, Yongho Shin, Ji-Ho Lee, Min Woo Jung, Jeong-Han Kim*

Department of Agricultural Biotechnology, Seoul National University

PFS-40 Antifungal and antiaflatoxigenic activities of naphthoquinones towards Aspergillus flavusGayoung Kim, Hye Seung Choe, Sung-Eun Lee*


PFS-41 Dissipation pattern of fungicide validamycin A in Sorghum bicolor after treatment of 20% water soluble powder during cultivation

Mai Rehan, Junghak Lee, Xiu Yuan, Seohyun Kim, Eunyoung Park, Jeong-Han Kim*


PFS-42 Hair growth promoting effect of 4HGF encapsulated with PGA nanoparticles (PGA-4HGF) by β-catenin activation and its related cell cycle molecules

Hye-Ji Lee, Ha-Kyong Kwon, Hye-Jin Park*

Department of Food Science and Biotechnology, College of BioNano Technology, Gachon University, Gyeonggi-do 13120, Republic of Korea

81



PAM-1 Optimization of multienzyme production from Aspergillus sp. for use in Retting Process of pineapple leaf fiber

Juthamas Suwanprateep, Paweena Thongkred*, Thidarat Nimchua*

BIOTEC, NSTDA

PAM-2 Conversion of l-arabinose to l-ribulose using genetically engineered Candida tropicalis In-Seok Yeo, Jung Hoe Kim*

Department of Biological Sciences, Korea Advanced Institute of Science and Technology

PAM-3 The prevalence of tetracycline-resistant bacteria and detection of ribosomal protective proteins (RPP) encoding genes of tetO, tetQ, tetS and tetW in soil samples collected from selected agricultural fields and animal houses in Gimje, Iksan and Jangsu areas

Kathyleen Nogrado1*, Heesu Jeon2, Jae-Young Cho1,2, Ji-Hoon Lee1,2 1Department of Agricultural Chemistry, Chonbuk National University, 2Department of Bioenvironmental Chemistry, Chonbuk National University

PAM-4 In Silico Optimization of 16S rRNA Gene Primer Pairs for Exploring Bacterial Diversity in Microbial Community Amplicon Analysis

Huy Quang Pham, Kerfahi Dorsaf, Yeon-Gyun Jung, Seung-Dae Choi, Minji Kim, Hyun-Woo Son,

Jae-Ho Shin*


PAM-5 Isolation and Characterization of IAA-producing Bacillus Consortia on Initial Growth Promotion of Arabidopsis thaliana and Lettuce (Lactuca sativa L.)

Jimin Lee, Hyeri Lee, Dong Gun Lee, Na-Hyun Lee, Sang Gyu Lee, Namhyun Chung*


PAM-6 Methods for Development of Bio-Fertilizers Containing Plant Growth-Promoting Rhizobacteria (PGPR) and Plant Growth Stimulants in Horticultural Crops

Na-Hyun Lee, Jimin Lee, Hyeri Lee, Dong Gun Lee, Sang Gyu Lee, Namhyun Chung*


82


PAM-7 Addition of Salt in the Incubation Media Induces Active Growth of the Symbiotic Bacteria in Entomopathogenic Nematodes

Awdhesh Mishra1, Yeon Su Yu2, Sang Heyong Park2, Kwang-Hyun Baek1* 1Department of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 38541, Republic of Korea, 2Research Division, Daedong Tech Co., Gyeongsan, Gyeongbuk 38401, Republic of Korea

PAM-8 Characterization of a fungal disease caused by Phialemonium curvatum in Lentinula edodes

Min Keun Kim*, Soon Ae Sim, Si Lim Choi, Kwang Pyo Hong

Environmental Agriculture Research Division, Gyeongnam Agricultural Research and Extension Services

PAM-9 Biocontrol Activity of Photorhabdus Species against the Streoptomycin-Resistant Plant Pathogenic Bacteria

Muhammad Fazle Rabbee, Md. Sarafat Ali, Kwang-Hyun Baek*

Department of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 38541, Republic of Korea

PAM-10 An integrated understanding of Xanthomonas pathogenesis controlled by two-component regulatory systems using transcriptome analysis

Mi-Ae Lee, Youngchul Yoo, Sujin Kim, Abdulwahab Antar, Sang-Won Lee*

Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University

PAM-11 Plasma activated water selectively enhances infected macrophage apoptosis Chaebok Lee1, Hwa-Jung Kim2* 1Department of Medical Science, Chungnam National University, 2Department of Medical Science and Department of Microbiology, Chungnam National University

83


Plenary Lecture


84


Determination of toxicodynamic differences of sodium channel isoforms to pyrethroids in juvenile and adult rat brain tissue

microtransplanted into Xenopus oocytes

John M. Clark*

Department of Veterinary & Animal Sciences, University of Massachusetts, Amherst, MA 01003-9301, USA

Microtransplantation of mammalian neurolemma is a tool to examine the endogenous structure and function of transmitter receptors and ion channels associated with the central nervous system. Microtransplanted neurolemma can originate from a variety of sources, possess ion channels and receptors in their native configuration, and are applicable to examine diseases associated with different channelpathies. In these experiments, we examined the age-related differences in VSSC expression and concentration-dependent responses to pyrethroids in juvenile and adult rat brain tissue microtransplanted in Xenopus oocytes. Automated western blotting results indicate that adult neurolemma exhibited 2.5-fold higher level of expression of VSSCs compared with juvenile neurolemma when normalized to the housekeeping protein β-tubulin. The predominate isoform expressed in both tissues was Nav1.2 with both showing a significant difference from zero. Adult neurolemma, however, expressed 2.8-fold more Nav1.2 than juvenile and also express Nav1.6 at a higher level (2.2-fold). In addition, neurolemma tissue microtransplanted into Xenopus oocytes showed reconstituted native ion currents in the plasma membrane of oocytes that was sensitive to TTX and abolished by choline ion replacement, functionally demonstrating the presence of VSSC. Increasing concentrations of permethrin and deltamethrin exhibited concentration-dependent increases in TTX-sensitive current from both adult and juvenile tissues. Concentration-dependent response curves were analyzed using the equivalence test and the slopes of the curves were different (p<0.05). VSSCs associated with juvenile neurolemma were up to 2.5X more sensitive to deltamethrin than VSSCs in adult neurolemma. In contrast, VSSCs from juvenile neurolemma were less sensitive than adult VSSSs at lower concentrations (0.6-0.8X) and more sensitive at higher concentrations (up to 2.4X). However, because the expected brain concentrations in humans following realistic exposure levels are approximately 21- (deltamethrin) to 333- (permethrin) times below the threshold for response in rat neurolemma, age-related differences, if any, are not likely to be toxicologically relevant.

PL-1

Special Lecture


86


Policy of Research Grant Support by Division of Life Sciences NRF

Young Yang*

Division of Life Sciences, National Research Foundation of Korea, Republic of Korea

In order to support basic research innovation for the next five years, the 4th basic research promotion comprehensive draft plan was established by Ministry of Science and Technology (MOST). Comprehensive plans are based on the following 10 key ideas. ① Provide a balanced support to all competent researchers. ② Support creative ideas freely. ③ Challenge the world’s best research. ④ The long-term perspective rather than the immediate results. ⑤ Balanced support to ensure that there are no areas to be neglected. ⑥ Prepare conditions for young researchers, who will be the future leaders, to grow. ⑦ Create an environment that can focus on research. ⑧ To spread a mature research culture based on trust. ⑨ Strengthen communication with researchers and citizens. ⑩ Strive to contribute basic research to the world. Detailed strategies for basic research innovation will be discussed.

I appreciate attendance of interested researchers and would like to hold a public hearing.

SL-1

Award Lectures


88


Targeting of cathepsin C in colorectal cancer cells

Sun Chul Kang*


Cathepsins are the lysosomal acid hydrolases, make crucial contributions to the tumor progression through a variety of different signaling mechanisms. The present work evaluated the efficacy of cathepsin C (CTSC) targeting on the anticancer potential of natural compounds. Curcumin treatment enhances the CTSC expression in colorectal cells. However, CTSC gene silencing by siRNA elevated the curcumin cytotoxicity in colorectal cancer cells. CTSC silencing and subsequent curcumin treatment upregulated autophagy while time dependent studies predicted that autophagy induction is primary event followed by apoptosis. Moreover, implications of natural compounds especially Epigallocatechin-3-gallate (EGCG) in the drug resistance reversal would be cherry on the cake. Recently we have uncovered the potential involvement of cathepsin C in drug resistance mechanism, and its targeting leads to the sensitization of drug-resistant colorectal cancer cells against conventional anticancer agents. In conclusion, the findings demonstrate that CTSC plays a significant role in colorectal cancer cell proliferation and its targeting can make cell susceptible to EGCG which demonstrate substantial promise for further development as a combinational drug with conventional anticancer agents for the treatment of various cancers.

AL-1

June 20 – 22 l Haeundae Grand Hotel, Busan, Korea 89

Award Lectures

Award Lectures

NITROGEN RESPONSE DEFICIENCY 1 positively regulates NRT1.1 for optimized growth in response to altered nitrate availability in

Arabidopsis

Won Je Lee1, Hai An Truong1, Cao Sơn Trịnh1, Seokjin Lee1, Suk-Whan Hong2, Hojoung Lee1*

1Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong 5-ga, Seongbuk-gu, Seoul 02841, Republic of Korea,

2Department of Molecular Biotechnology, College of Agriculture and Life Sciences, Bioenergy Research Center, Chonnam National University, Gwangju 61186, Republic of Korea

Plants cannot fix nitrogen directly; therefore, they must absorb nitrogen from the soil through their roots. However, the efficiency of nitrogen use in crops is low; more than half of the available nitrogen often leaches into the environment. Understanding how nitrogen signaling pathways are regulated is essential to the process of maximizing the efficiency of nitrogen use in crops. We have characterized the newly identified Myb-like gene, NITROGEN RESPONSE DEFICIENCY 1 (NID1). We observed that growth performance in nid1 knockout (ko) mutant plants was better than that of wild type Col-0 plants under very low-nitrate conditions, which led to improved growth performance in the nid1 ko plants. On the basis of ChIP and EMSA experiments, it is understood that NID1 binds to the promoter of the NRT1.1 gene. Furthermore, nid1 ko plants exhibited similar growth performance with nrt1.1 ko (chl1-5) plants in response to nitrate-free conditions. In this study, we suggest that NID1 plays a crucial role as a transcription factor in optimizing plant growth by controlling the expression of the nitrate transporter NRT1.1 as well as the ABA biosynthesis genes in response to low nitrate condition.

AL-2

91




IS1Biochemistry · Molecular Biology

IS2Environmental Sciences

IS3Natural Products · Bioactive Materials · Biomedical Sciences

IS4Food Sciences

IS5Applied Microbiology

92


Improving end-use quality and immunogenic potential of wheat flour

Susan B. Altenbach*

USDA-ARS Western Regional Research Center, Albany, CA, USA

Wheat is a major food crop grown throughout the world that is used in a wide range of different food products because of the unique viscoelastic properties of the flour. These properties are conferred by the gluten proteins, a complex group of proteins that are unusual in that they contain large regions of repetitive sequences with high proportions of glutamine and proline. The gluten proteins are divided into gliadins and glutenins that confer extensibility and elasticity, respectively, to wheat flour dough. The monomeric gliadins consist of alpha, gamma, delta and omega types while the glutenins form large insoluble polymers composed of high molecular weight glutenin subunits (HMW-GS) and low molecular weight glutenin subunits (LMW-GS) that are linked by disulfide bonds. Recent data from genome sequencing studies revealed 26 alpha, 11 gamma, two delta, five omega gliadin and 10 LMW-GS genes that encode full-length proteins in the reference wheat Chinese Spring in addition to the four HMW-GS genes already characterized. Ultimately, the composition of the gluten proteins in the flour, determined by both the genetics of the plant and the growth environment, is critical for end-use quality. Many gluten proteins also trigger a number of important human health conditions, including celiac disease, IgE-mediated food allergies and non-celiac wheat sensitivities.

A better understanding of the relationships between specific gluten proteins and their contributions to human health conditions and end-use functional properties is important for efforts to develop wheat that will be either less likely to trigger immunogenic responses or might be better tolerated by patients with celiac disease and food allergies. Towards this end, we have suppressed the expression of genes encoding three different groups of highly immunogenic proteins in a commercial U.S. wheat cultivar using RNA interference. Genes targeted include the omega-5 gliadins, the major sensitizing allergens in the serious wheat allergy wheat-dependent exercise-induced anaphylaxis (WDEIA), and the omega-1,2 gliadins and alpha gliadins that contain important epitopes involved in celiac disease. Effects of genetic modifications on flour protein composition, end-use properties and allergenic potential will be discussed. The results suggest that biotechnology approaches can be used to improve the healthfulness of wheat, while maintaining or even improving its end-use qualities.

IS1-1




Direct role of MUTE in orchestrating stomatal differentiation

Soon-Ki Han1*, Keiko U. Torii1,2,3

1Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Chikusa, Nagoya 464-8601, Japan, 2Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195-1800, USA,

3Department of Biology, University of Washington, Seattle, WA 98195, USA

Plant stomata are cellular valves composed of paired guard cells that impact plant growth, sustenance, and atmospheric environment. For the differentiation of a functional stoma, a single symmetric division of an immediate precursor is absolutely essential. We have revealed the comprehensive inventories of transcriptional changes driven by the Arabidopsis bHLH protein MUTE, a potent inducer of stomatal differentiation. MUTE switches the gene expression program initiated by its sister bHLH, SPEECHLESS, thereby ‘locking in’ the differentiation program while taking over the shared targets. MUTE directly induces a suite of cell-cycle genes and their direct transcriptional repressors. The architecture of the gene regulatory network initiated by MUTE represents an Incoherent Type 1 Feed-Forward Loop, which yields a robust, sharp pulse of output response. Our mathematical modelling and a series of experimental perturbations support a notion that MUTE orchestrates the transcriptional cascade leading to a tightly-restricted, robust pulse of cell-cycle gene expression, thereby ensuring the single cell division to make stomata. Currently, we are further characterizing the promising MUTE target genes aiming to elucidate the molecular mechanism of how MUTE terminates self-renewing cell state and specifies cell fate in response to endogenous signaling.

IS1-2

94


In vivo Imaging of Reactive Oxygen Species (ROS) Using a New Ratiometric ROS Bio-reporter for Studying the Function of ROS

Su-Hwa Kim, Won-Gyu Choi*

Department of Biochemistry and Molecular Biology, University of Nevada, Reno, NV 89557, USA

Reactive oxygen species (ROS) are spontaneously generated during plant growth and when plants experience various stresses. Although accumulation of ROS can be toxic to plants, it also acts as signaling molecules, which are closely associated with adapting to stresses. Tight regulation of ROS homeostasis is required to adapt to stress and survive. However, in vivo spatiotemporal information of ROS dynamics is still largely undefined, in part due to the limited range of available ROS bioreporters. In order to understand the dynamics of ROS changes and their biological function in adapting to stresses, a quantitative ROS transcription-based bioreporter was developed using a promoter fusion stratagem. This bioreporter uses a ROS-responsive promoter from ZAT12 to drive Green Fluorescent Protein (GFP) expression and to compare the resulting expression to a constitutively expressed Red Fluorescent Protein (mCherry). The ROS-bioreporter, ZAT12p-ROS, was used to assess ROS response to oxidative stress (H2O2), salt stress (100 mM NaCl), and pathogen related elicitor flg22. The ZAT12p-ROS bioreporter showed increases in the ratio values of GFP to mCherry signals within 10 to 30 min post stress treatments, consistent with stress-induced ROS accumulation in the hypocotyl and root. Such stress-associated ROS signals correlated with the induction of abiotic/biotic stress responsive markers such as RbohD, ZAT12, SOS2, and PR5 suggesting the ZAT12p-ROS provides a robust indicator of increased ROS, which is related to stress responses. Based upon the temporal response patterns and magnitude of signal increases, the ZAT12p-ROS bioreporter appears to be suitable for cellular mapping of ROS changes in response to abiotic and biotic stresses.

IS1-3




Target Identification of Bioactive Molecules

Qing X. Li*

Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, Hawaii 96822, USA

Molecular target identification is pivotal in nutraceutical and pharmacological research, and drug and pesticide discovery. It involves identification and characterization of a specific molecular target to which a bioactive compound binds, such as an enzyme or receptor. Agrochemicals are used to increase crop production and food quality. Nutraceuticals are products derived from food sources with extra health benefits, in addition to the basic nutritional value found in foods. Agricultural and therapeutic research synergistically shares the same goal – human health, which is accomplished from one health approach covering from healthy environment, to safe and nutritious foods, to healthy lifestyles and advanced medicines. Since the establishment in 1960, the Korean Society for Applied Biological Chemistry (KSABC) has become a representative forum of the Korean applied biological chemistry and the related industry. KSABC is also a “network hub” to link biochemistry, molecular biology, environmental sciences, natural product, bioactive materials, biotechnology, food sciences, applied microbiology, and agricultural biologicals and related areas.

Three cases will be presented to indicate how molecular targets have been identified. First, molecular screening revealed that the C-glycosylflavone isoorientin inhibits glycogen synthase kinase-3β (GSK-3β). GSK-3β is a key enzyme catalyzing hyperphosphorylation of tau protein. To our surprise, isoorientin specifically inhibits GSK-3β via a substrate competitive mode, rather than the common ATP competitive mode. It effectively attenuates GSK-3β-catalyzed tau hyperphosphorylation and is neuroprotective against amyloid-induced neurotoxicity in human SH-SY5Y cells. It is known that hyperphosphorylation of tau proteins in neurons plays a pivotal role in Alzheimer’s disease (AD) pathology. AD is the most frequent neurodegenerative disorder in the aged population, which cannot be prevented, cured or even slowed.

The second is to use quantitative proteomics to identify tentative molecular targets. 24-Methylenecyloartanyl ferulate (24-mCAF) isolated from rice bran oil was found to be cytotoxic to A549 cells that are a non-small cell lung cancer (NSCLC) cell line. An iTRAQ-based quantitative proteomics analysis suggested that 24-mCAF inhibits cell proliferation and activates cell death and apoptosis. 24-mCAF induces up-regulation of Myb binding protein 1A (MYBBP1A), a tumor suppressor that halts cancer progression. In vitro enzymatic assays confirmed that 24-mCAF inhibits the activity of AKT and Aurora B kinase, two Ser/Thr kinases involved in MYBBP1A regulation and that may represent important targets in NSCLC. Lung cancer is the second most prevalent cancer. NSCLC is the most common type of lung cancer. The low efficacy in current chemotherapies impels to find new alternatives to prevent or treat NSCLC.

Finally, a potential target was first hinted by acute toxicity tests. Linalool, estragole, and methyl eugenol are the active chemicals in many essential oils, particularly basil oils. Basil oil and the aforementioned monoterpenoids have been widely used for health benefits as well for pest insect control. However, the molecular target of those chemical constituents is not well-understood. It is well-known that the γ-aminobutyric acid type A receptors (GABAAR) and nicotinic acetylcholine receptor (nAChR) are primary molecular targets of synthetic insecticides used in the market today. We studied the electrophysiological effects of linalool, estragole, methyl eugenol, and citronellal on GABAAR and nAChR to further understand their versatility as traditional medicines and as insecticides. Our results revealed that linalool inhibits both GABAAR and nAChR, which may explain its insecticidal activity. Linalool is a concentration-dependent, non-competitive inhibitor on the GABAAR as evidenced by negligible effects of linalool on the EC50 values of GABA for the rat α1β3γ2L GABAAR. The half maximal inhibitory concentration (IC50) of linalool on the GABAAR was approximately 3.2 mM. As multiple monoterpenoids are present in the same essential oil, it is likely that linalool synergistically interacts with methyl eugenol (and other monoterpenoids) as both a GABAAR agonist and positive allosteric modulator, thus putatively explaining for its sedative and anticonvulsant effects.

IS2-1

96


Heavy Metal Contamination in Soil-Water Plant System and Their Human Health Risk Assessment in Peri-Urban Areas of Bangladesh

M. Mokhlesur Rahman*, M. Taufique Arefin

Department of Agricultural Chemistry, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Metal contamination of agroecosystem has become a serious environmental problem due to uncontrolled long-term disposal of untreated industrial effluents containing toxic metals and indiscriminate use of metal-containing fertilizers in agricultural fields. As a consequence, heavy metal contamination of soils by the polluted river water is posing an increasing threat to surface water irrigation, which causes toxic effect on soils and crops ultimately entering foods and also creates environmental problem affecting agricultural production, food quality and human health. The aim of this present study was designed to explore the intensity of heavy metal contamination in soils and crops at Turag, Balu, Khiru and Bangshi river sites irrigated with industrially contaminated river water at farmers’ field during dry season toward food safety for human consumption. Sixty water samples of each river were collected randomly to measure pH, EC, TDS and metal status (Cd, Cr, Cu, Fe, Mn, Ni, Pb and Zn) in order to categorize their suitability for irrigation usage. Again, sixty soil samples were collected to determine soil texture, pH, EC, OC and metal ions such as Cd, Cr, Cu, Fe, Mn, Ni, Pb and Zn. Plant samples were also collected from the adjacent crop fields irrigated with the contaminated river waters and were analyzed to determine the levels of metal ions like Cd, Cr, Cu, Fe, Mn, Ni, Pb and Zn. pH values of four river water samples were slightly acidic to alkaline in nature. The contaminated four river water samples were under low to medium salinity (C1, EC<250 µS cm-1 & C2, EC=250-750 µS cm-1) and alkalinity (S1, SAR<10) hazards expressing as C1S1 and C2S1. As regards to the measured TDS values, river water samples under test were rated as fresh water (TDS<1,000 mg L-1) in quality. The levels of Mn and Cr ions in four rivers under study exceeded the safe limit of WHO/FAO standard and were considered as contaminants for irrigation usage. The order of heavy metals magnitude for four rivers was as: Fe>Mn>Cr>Pb>Zn>Cu>Ni for Turag river, Fe>Mn>Pb>Cr>Zn>Cu>Ni for Balu river, Fe>Pb>Mn>Zn>Cr>Cu>Ni for Khiru river and Fe>Mn>Cr>Pb>Cu>Zn>Ni for Bangshi river. In order to better understanding the relationships between soil characteristics and heavy metal mobility, the obtained results showed the strong influence of pH, EC and soil texture on retention and mobility of heavy metals. On the basis of heavy metal levels, soil irrigated with the contaminated river water under investigation was found in the order of Fe>Mn>Zn>Cu>Cr>Pb>Ni for Turag; Fe>Mn>Zn>Cr>Cu>Pb>Ni for Balu; Fe>Mn>Zn>Cr>Pb>Cu>Ni for Khiru and Fe>Mn>Zn>Cr>Pb>Cu>Ni for Bangshi. Among the identified metals, the accumulations of Cr, Mn and Pb ions were detected in the contaminated soils irrigated with river waters and these metal ions were treated as soil contaminants due to long-term irrigation usage. Geoaccumulation index (Igeo) values of four river sites indicated polluted to moderately polluted soil in terms of heavy metals while pollution load index (PLI) indicated soil environment of those river sites with progressive deterioration. The calculated enrichment factor (EFc) values of four river water sites exhibited the enrichment of Cr and Pb in soils. The sequence of soil pollution level of the studied four river areas were Bangshi>Turag>Balu>Khiru. Multivariate analysis revealed the correlation of chemical parameters of soil and water samples loading by the contaminated river water irrigation. The bioaccumulations of Pb, Ni and Cr in leafy vegetables viz., water spinach, red amaranth, indian spinach and stem amaranth were recorded in three rivers sites and bioaccumulations of Pb and Cr in rice grain and straw were also found in four river areas for long-term irrigated sites. Heavy metal contamination of vegetables, rice grain and straw grown around four river sites would pose a great health risk to the local population through dietary intake for long-term consumption. Considering the scenario of total target hazard quotient (TTHQ), health risks associated with the consumption of vegetables in three rivers sites as TTHQ values were in the order of Turag>Balu>Bangshi river while the TTHQ of rice grain in Khiru river site was 1.23 indicating potential health risks. From the research findings, it is concluded that the contamination of soil, water and plant systems caused by heavy metals under consideration should not be ignored in the irrigated sites of four rivers under study toward food safety and health risks.

IS2-2




Discovery of Secondary Metabolites with Nematicidal Activity from Higher Fungi against Root-knot Nematodes

Jin-Cheol Kim*

Department of Agricultural Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of Korea

Root-knot nematodes (RKN; Meloidogyne spp.) are one of the most economically damaging group of plant-parasitic nematodes worldwide. RKN is responsible for a large part of global damage to reach $100 billion caused annually by nematodes. In order to discover new nematicidal metabolites and develop new microbial bionematicides using higher fungi, we examined nematicidal activities more than 4500 fungal culture filtrates against juveniles of Meloidogyne incognita, which is a causal agent of root knot disease on various crops. Through the screening, we found that the culture filtrates of two fungal strains such as Aspergillus niger F22 and Xylaria grammica KCTC 13121BP were active to M. incongnita with marked mortality of second-stage juveniles and inhibition of egg hatching. The nematicidal metabolite from the culture filtrate of A. niger F22 was identified as oxalic acid by organic acid analysis and gas chromatography-mass spectroscopy (GC-MS). Exposure to 2 mmol/L oxalic acid resulted in 100% juvenile mortality at 1 day after treatment and suppressed egg hatching by 95.6% at 7 days after treatment. Oxalic acid showed similar nematicidal activity against M. hapla, but was not highly toxic to Bursaphelenchus xylophilus. A new microbial nematicide named “Nemafree” using A. niger F22 was commercialized from March 2017. On the other hand, bioassay-guided fractionation from the culture filtrate of an endolichenic fungus X. grammica EL000614 and instrumental analyses led to grammicin being identified as the nematicidal metabolite. Grammicin showed strong second-stage juvenile killing and egg-hatching inhibitory effects, with a 50% effective concentration at 72 h (EC50/72 h) of 15.9 μg/mL and a 50% effective concentration at 14 days (EC50/14 days) of 5.87 μg/mL, respectively. In pot and field experiments, a wettable powder-type formulation and fermentation broth filtrate of X. grammica KCTC 13121BP effectively suppressed the development of RKN disease on tomato and melon plants. Additionally, the optimization of the fermentation condition for grammicin production by X. grammica EL000614 was carried out. The maximum amount of grammicin in a minimal medium-based optimum medium in a flask was 5.75 g/L after 3 weeks fermentation, which is approximately 4 times higher level than potato dextrose broth (PDB) medium culture. The optimum culture (OC) filtrate also showed 4 times stronger nematicidal activity against 2nd juveniles of M. incognita and M. arenaria than PDB culture filtrate. The results suggest that X. grammica and grammicin may have potential applications for control of RKN disease of various crops. Further research on the development of fermentation and formulation processes, and evaluation of disease control efficacy against various nematode diseases are necessary for the development of new bionematicides.

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98


Mechanisms mediating anti-inflammatory effects of dietary bioactive compounds in obesity and insulin resistance

Naima Moustaid-Moussa*

Department of Nutritional Sciences, Obesity Research Institute, Texas Tech University, Lubbock, TX, USA

Obesity is a complex disease that is associated with the expansion of white adipose tissue, creating a system in which the body progressively transitions into an inflammatory state. Obesity also leads to several metabolic disorders including diabetes and cardiovascular diseases, all of which are also characterized by chronic low-grade inflammation. As a result, bioactive compounds with anti-inflammatory properties have gained significant attention as anti-obesity targets. Some of these dietary components also increase brown adipose tissue activity and/or browning of white adipose tissue.

Our lab has extensively studied the mechanistic basis for anti-inflammatory effects of various dietary and plant bioactive compounds including omega 3 polyunsaturated fatty acids, tart cherry anthocyanins, tocotrienols, and botanicals in white and brown adipose tissues using diet-induced obese mice. We previously reported that mice fed high fat diets supplemented with the omega-3 polyunsaturated fatty acid, eicosapentaenoic acid, (HF-EPA) exhibited reduced adipose and systemic inflammation, improved glucose clearance and insulin resistance, and revered fatty liver, compared to mice fed high fat diets without EPA (HF). Tocotrienols and tary cherry anthocyanins also exhibited potent anti-inflammatory effects in white adipose tissue.

Mechanisms mediating the beneficial effects of omega 3 fatty acids in both white and brown adipose tissue included decreased expression of adipogenic, lipogenic and inflammatory genes and increased lipid oxidation, leading to adipocyte hypotrophy and lower macrophage infiltration in adipose tissue in mice fed high fat diets supplemented with EPA compared to non-supplemented mice. We have also demonstrated that EPA increased oxygen consumption and the thermogenic protein, uncoupling protein 1 (UCP1) and upregulated expression of other master regulators of thermogenesis in brown adipose tissue. Unexpectedly, using UCP1-deficient mice, we found that these protective effects of EPA were independent of UCP1.

To further dissect mechanisms of EPA effects in obesity, we used RNA and small RNA sequencing to profile differential gene and miRNA expression in white and brown fat. We identified several gene-miRNAs pairs that may mediate metabolic effects of EPA in adipose tissue. In white adipose tissue, several of these pairs mapped to pro-inflammatory pathways such as NF-κB; while in brown adipose tissue, pathways related to thermogenesis such as peroxisome proliferator-activated receptor (PPAR) signaling were most prominent. Thus, the depth of transcriptomic and miRNA profiling revealed novel mRNA-miRNA interaction networks in both white and brown adipose tissues that may mediate effects of EPA on energy homeostasis.

In conclusion, natural compounds such as omega 3 fatty acids are important regulators of energy metabolism. Moreover, global genomic studies are important in identifying novel pathways regulated by these compounds that may lead to novel targets to prevent/treat metabolic complications associated with obesity.

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Cannabis in California, USA

Gyunghoon Hong*

Bureau of Cannabis Control, Department of Consumer Affair, Sacramento, CA, USA

As of January 2019 in the United States, adult-use cannabis is legal in 10 states and medical cannabis is legal in 33 states, the District of Columbia, and four out of five U.S. territories.

On November 8, 2016, California voters approved Proposition 64, thus legalizing the adult usage, possession, and cultivation of cannabis. California had already legalized the use of medical marijuana 20 years ago, by passing Proposition 215.

In response to the legalization, three government agencies (CDFA, CDPH, BCC) have established a licensing structure and framework for regulating adult use and medical cannabis activities.

This presentation will introduce each aspect of the cannabis business infrastructure, including but not limited to their main activities, how they relate to one another, and the appropriate regulations. An introduction to the cannabis plant, major cannabinoids and their medical effects, and the endocannabinoid system will also be discussed.

Because the cannabis plant is still listed as a Schedule I substance of the Controlled Substances Act established by the federal government, research pertaining to cannabis has been strictly limited. Although there have been many reports of the medical effects of cannabis or cannabinoids that are extracted from the cannabis plant, there is currently insufficient data to support the safety and validity of these effects.

Adult use cannabis were legalized for the past two years from 2017 to 2018 in California under emergency regulations. Beginning in 2019, fully adapted regulations are in effect for the entire cannabis market. This is just the beginning for cannabis in the state of California. With the advance of more research and experiments on cannabis, the demand of cannabis-related products could skyrocket not only for patients, but also for adult use consumers in the near future.

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100


Optimization of compound mixtures via ex vivo fluorescence monitoring for improved cellular delivery and effects

Hyejung Mok*

Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea

It is crucial to understand how natural compound mixtures work together in cells and in vivo to improve their clinical efficacy and therapeutic effects. In this presentation, synergistic effects of compound mixtures were examined in terms of intracellular uptake and bio-distribution using curcumin and umbelliferone derived from Angelica (A.) gigas. Localization of these natural compounds was easily monitored by fluorescence imaging ex vivo in the presence of different types of compounds. After administration of natural compound mixtures intravenously, fluorescent intensities in cells and tissues were visualized using in vivo imaging systems. Bioactivities in vitro e.g. antioxidant activities and cellular reactive oxygen species levels were examined in the presence of other compounds for macrophages. To elucidate molecular mechanism of compound mixtures, inhibition of the efflux pump P-glycoprotein (P-gp) and stability of natural compounds were quantitatively analyzed in vitro. Taken together, compound mixtures were optimized for enhanced intracellular localization and enzymatic stability, which might lead to improved bioactivity of natural compounds for therapeutic applications.

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The dangers of hidden fat: bringing intra-pancreatic fat deposition to the fore

Max S. Petrov*

School of Medicine, University of Auckland, Auckland 1023, New Zealand

It is well established that obesity (defined as excess fat mass) increases risks for several diseases, including but not limited to diabetes mellitus, cardiovascular diseases, and cancer. Emerging evidence indicates that, over and above fat mass per se, the pattern of fat distribution has a profound influence on systemic metabolism and health outcomes. Variations in the capacity of different depots to store and release fatty acids and to produce adipokines are important determinants of fat distribution and its metabolic consequences. Depot differences in cellular composition and physiology, including innervation and blood flow, likely influence their phenotypic properties. Several studies showed that adipocytes from different anatomical sites are intrinsically different as a result of genetic or developmental events. This presentation will put a spotlight on the phenotypic characteristics of fat deposition in the pancreas and mechanisms that link its depot-specific biology to metabolic disorders.

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102


Safety Issues of Food and Agricultural Products in Kazakhstan

Jannat Iskakova1*, Zhelderbayeva Makhabbat2

1Candidate of Agricultural Sciences, Associate Professor of “Food technology and safety” Department, Kazakh National Agrarian University, Kazakhstan,

2Candidate of Medical Sciences, Kazakh National Medical University by Asfendiarov, Kazakhstan

Agriculture is an important sector of Kazakhstan's economy. The area of agricultural land of the country is 76.5 thousand hectares, including arable land — 24.1 million hectares, pastures — 46.5 thousand hectares. In the North, climatic conditions favor for the cultivation of spring wheat, oats, barley and other crops, as well as allow the development of vegetable growing, melons and cultivate a number of industrial crops — sunflower, flax, tobacco. In the South of the Republic, in the foothills and river valleys, where a lot of heat, with artificial irrigation give high yields of cotton, sugar beet, yellow tobacco, rice, fruit orchards and vineyards. Natural conditions of Kazakhstan, their diversity cause significant potential opportunities for the development of livestock. The Republic traditionally engaged in sheep, horse, camel, cattle breeding. Kazakhstan is among the top ten world exporters of wheat (sixth place in 2011-2012). The country has fairly good land, but its territories suffer from environmental obstacles such as lack of water and harsh climatic conditions. In grain-growing areas are cultivated mainly strong and hard wheat varieties with a high content of gluten, such wheat is in great demand on the world market. Since the mid-2000s, Kazakhstan has been a net importer of agri-food products.

The implementation of the concept of economic development involves ensuring food security. In 2017, exports of the food industry of Kazakhstan by 44% consisted of processed products, the remaining 56% accounted for raw materials (wheat, barley, etc.). As for imports of food products, the ratio of processed and raw materials was 70% to 30%. Thus, ensuring food and food security of the state is a strategic task for the Government of Kazakhstan. The problem of food security includes political, social, technical, economic, medical and other aspects.

The country’s land resources was in critical condition. Fertile arable land was in depletion. There is a desertification of pastures. Air pollution, especially in large industrial centres, has reached alarming levels. These include the zone of ecological disaster, which are the Aral and Semipalatinsk regions, where the destruction of natural ecological systems, degradation of flora and fauna. It should also be noted that Kazakhstan is classified as a country with a large water deficit. But despite this, our natural reservoirs are heavily polluted by enterprises of mining, metallurgical and chemical industries, municipal services of cities and pose a real environmental threat. Also the intensification of agricultural production, the desire to get more out of a unit of acreage make to active use of agrochemicals, genetically modified plants, hormones and growth stimulants. And their use increases the risk of poor quality and dangerous food products, from which in our time of rapid logistics can suffer whole Nations.

On the territory of Kazakhstan until the 90-ies pesticides were used in almost the entire area. Pesticides with POPS properties have never been produced in Kazakhstan. They are not currently imported or exported, nor are they planned to be used, imported or exported in the future. However, large amounts of POPS previously produced and used in the former USSR have been accumulated in most of Kazakhstan.

However, up to the 90s DDT was used in Kazakhstan in veterinary medicine. Small residues of DDT are still found in soil, plants and water, air in the work area and food.

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Priority issues related to chemicals in Kazakhstan include: - air pollution (SO2, NOx, CO, dust, O3, PAH), - presence of hazardous chemicals in agricultural food products (pesticides, nitrates)- pollution of drinking water (heavy metals, oil products), - processing/destruction of hazardous waste (radioactive waste, obsolete pesticides, ash and other), - occupational health in agriculture (pesticides, fertilizers), - pollution of inland waters and waterways (PAHs, phenols, heavy metals, pesticides, Unions), - pollution of groundwater (pesticides, petroleum products, PCBs, heavy metals), - soil pollution (oil products, pesticides, heavy metals).In 2018, 58855 objects producing and selling food products were under control (2017. – 57435), of which 7553 or 12.8%

were examined. Above the national average of surveys was in Aktobe – 32.9%, Pavlodar – 26.1%, North Kazakhstan – 23.0%, Karaganda – 20.1%, Almaty – 14.3% and Shymkent – 15.5% regions.

6558 food objects or 86.8% were examined with the use of laboratory methods. The number of objects that do not meet the requirements of sanitary regulations – 252 or 3.3 %.

In 2018 territorial authorities public health rejected and removed from realization 1077956,77 kg (2017. – 297383,23 kg) of food and raw materials, of which produced in RK – 798230,09 kg or 74,1% (2017. – 82588,27 kg) imported from other countries – 279726,7 kg or 25.9% (2017. – 214795 kg).

In comparison with 2017, the consumer market of Kazakhstan received 3.6 times more of substandard and unsafe food products for the health of the population. In 2018, compared with 2017, there was an increase in controlled safety indicators that do not meet the requirements of sanitary rules in the controlled epidemiologically important food facilities. In the Republic, the number of outbreaks of acute intestinal infections and food poisoning has increased dramatically, by 1.5 times compared to 2017.

The consumer protection Committee of the Ministry of national economy of the Republic of Kazakhstan at the end of January 2019 stated that 35.8% of fish products and 27.1% of meat products, as well as 22.7% of bakery products sold in Kazakhstan, do not meet the safety and quality requirements. During the year, with monthly monitoring at the stage of implementation, more than 29 thousand samples of food were selected and examined. According to the Committee, 86% does not meet standards of food production are accounted for by imports. The products of domestic manufacturers that do not meet the requirements amounted to 14%.

The main segment of unsafe and substandard food products falls on the share of imported producers: from the Russian Federation – 52%, Kyrgyzstan – 4%, the Republic of Belarus – 3%, Ukraine – 2.5%, China, Turkey, Latvia, USA, Moldova, Georgia, France, Uzbekistan and other countries – up to 1%. 17.5 tons of dangerous food products were removed from the sale.

One of the key conditions for the participation of the exporting country in international food trade is the country's ability to ensure the quality and safety of its products.

104


Functional thermotolerant yeasts isolated in Vietnam for ethanol and fruit wine production

Ngo Thi Phuong Dung1*, Huynh Xuan Phong1, Mamoru Yamada2, Pornthap Thanonkeo3

1Biotechnology Research and Development Institute, Can Tho University, Can Tho, Vietnam, 2Faculty of Agriculture and Graduate School of Science and Technology for Innovation, Yamaguchi University,

Yamaguchi, Japan, 3Faculty of Technology, Khon Kaen University, Khon Kaen, Thailand

In Vietnam, ethanol is concerned in many different products in terms of fermentation technology including alcoholic drinks and bio-fuel. Due to the challenges of increasing global temperature and benefits associated with processing at high temperature, using thermotolerant yeasts for ethanol fermentation is a potential orientation in tropical countries as Vietnam. In our recent studies, the diversity of thermotolerant yeast isolates performing high capacities and stability for the controlled processing of alcoholic winemaking and ethanol production from the cheap and available raw materials in region has been addressed and achieved.

A total of 712 yeast isolates was purified from many different kinds of raw material sources in the Mekong Delta, Vietnam; such as ripe fruits, flowers of fruit-tree, cocoa, fermented products, alcoholic fermentation starters, sugarcane, molasses, sawdust, agricultural by-products and soil samples. All of these yeast isolates could grow well at 37ºC and there were about 80%, 45% and 10% of yeasts could grow at 40ºC, 43ºC and 45ºC, respectively. More than 80% of yeasts were able to grow in the medium containing 9% (v/v) of ethanol and it was decreased to about 40% of yeasts growing in the medium supplemented 12% (v/v) of ethanol. Due to the thermotolerance and ethanol fermentation capacity at high temperature, 88 yeast isolates were selected and characterized. The predominantly abundant identified species include Candida tropicalis, Saccharomyces cerevisiae, Pichia kudriavzevii, and Candida glabrata. The database of these selected yeasts has been systemized and recorded including information on morphological, physiological and biochemical characteristics; thermotolerant ability; ethanol tolerance; fermentation capacity and assimilation ability of carbon sources; DNA sequence results and names of identified species.

With the aim to pave the way for the application of useful thermotolerant ethanologenic yeasts towards the industrial fermentation technology, the technical feasibilities for the ethanol production and the winemaking by using the selected thermotolerant yeasts have also been investigated. The fermentation capacity of the tested yeasts was monitored and compared at different temperatures (37ºC, 40ºC, 43ºC). Then the selected yeasts were further examined for the optimum fermentation conditions at 37ºC in a factorial design with three factors at three levels, including yeast inoculation level, initial sugar concentration and fermentation time. For the ethanol fermentation, sugar-cane juice and molasses were employed as raw materials. The highest ethanol concentration could be found at about 10.5% (v/v) and up to 5.5% (v/v) during the fermentation at 37°C and 40°C, respectively. For wine manufacture, the different kinds of fruits were employed as raw materials such as: pineapple, watermelon, dragon fruit, guava, and three-leaved wild vine. The highest ethanol concentration of the final wine product could reach about 12% (v/v) and up to 7% (v/v) during the fermentation at 37°C and 40°C, respectively.

The research findings on the diversified collection of thermotolerant ethanologenic yeasts isolated from Vietnam and the processing feasibility indicate the promising application of such newly functional yeasts for the controlled ethanol production at high temperatures from agricultural by-products and the winemaking manufacture from different available fruit resources in region. The further advanced study on the expression levels of the selected genes and the metabolic pathways will also be studied to explore the regulation of these genes to get maximum benefits of the superior thermotolerant yeasts for the high-temperature ethanol production.

IS4-3




Anaerobic ammonium oxidizing (anammox) bacteria – from ecophysiology to practical application –

Satoshi Okabe*

Division of Environmental Engineering, Faculty of Engineering, Hokkaido University, North-13, West-8, Sapporo, Hokkaido 060-8628, Japan

Anaerobic ammonium oxidation (anammox) is a microbial process where NH4+ is anaerobically oxidized to N2 gas with

NO2– as an electron acceptor. This novel pathway of nitrogen cycle was predicted, but not experimentally proven.

Undiscovered anammox bacteria were finally discovered in early 1990s. Since then extensive studies have been conducted to reveal their physiology, biochemistry, and ecology, and it is now known that anammox bacteria are ubiquitously detected from anoxic natural and man-made ecosystems and key players in the nitrogen cycle. For example, anammox process significantly contributed to nitrogen loss; e.g., the anammox process accounted for nearly 100% of total nitrogen loss in anoxic water column of the Namibian oxygen minimum zone (OMZ) and up to 79% of total nitrogen loss in the marine sediments. However, our understanding is largely limited due to a lack of pure culture of anammox bacteria.

The nitrogen removal process using this newly discovered anammox bacteria has been recognized as a promising cost-effective and low energy alternative to the conventional nitrification-denitrification processes due to a significant reduction of aeration and alkalinity for nitrification and organic carbon for denitrification. To our best knowledge, there are probably more than 114 reported full-scale anammox treatment plants around the world. Anammox-based process is, however, not yet recognized as popular nitrogen removal process like the conventional nitrification-denitrification processes. There are still some challenges in practical application of anammox-based treatment process at full-scale, e.g., longer start-up period, limited application to mainstream municipal wastewater and poor effluent water quality.

In this presentation, current our knowledge of anammox bacterial ecophysiology and engineering application of the anammox process for wastewater treatment will be presented.

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106


Banking and Characterizing Human Gut Microbiome

Ju Huck Lee*, Byeong Seob Oh, Seung Yeob Yu, Ji-Sun Kim, Seung-Hwan Park, Se Won Kang, Jam-Eon Park, Seung-Hyeon Choi, Kook-Il Han, Keun Chul Lee, Mi Kyung Eom, Min Kuk Suh, Jung-Sook Lee

Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, Republic of Korea

Since a USA National Institutes of Health launched the Human Microbiome Project (HMP) in 2007, the human microbiome has been gaining increasing attention in the world. Furthermore, in addition that human gut microbiome has been known to play an important role in a biological metabolism and digestive capacity, recent studies revealed that it is implicated in a variety of diseases such as inflammatory bowel diseases, colorectal cancer, and interestingly even neuronal disorders. The human microbiome, therefore, has been considered as a forgotten organ.

In November 2016 Biological Resource Center in KRIBB initiated the Korean Microbiome Project like HMP called Korean Gut Microbiome Bank (KGMB) to investigate healthy Korean gut microbial flora, which can be used as reference for the study of microbiome related diseases for Korean. Interestingly, unlike other country national microbiome projects which was performed only by culture-independent methods, culture-dependent methods producing real microbiome resources have been applied to the KGMB because the need of the real microbiome resources has increased recently in the microbiome fields in which the culture-independent methods were mostly done. This talk will introduce and discuss the KGMB. Furthermore, the characterization of human microbiome involved in several disease including colorectal cancer will be also discussed.

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Regulation of Plant and Human Immunity by Interaction of Salicylates with Their Target Proteins

Hyong Woo Choi*

Department of Plant Medicals, Andong National University, Andong 36729, Republic of Korea

Salicylic acid (SA) plays a key roles in plants, including plant development, stomatal closure, thermogenesis, and response to abiotic and biotic stresses. Its role in plant immunity is the most extensively studied, but it is still only partially understood. In plants, more than two dozens of SA-binding proteins (SABPs) are identified based on the classical biochemical approaches and genome-wide high-throughput screens. Interestingly some of these proteins exhibited high affinity against SA, while the others showed low affinity, in vitro. Presence of SABPs exhibiting a wide range of affinities for SA may provide great flexibility and multiple mechanisms through which SA can act. Importantly, similar screening of human proteome identified several target proteins of SA and its natural and synthetic derivatives (Salicylates). Many of these human proteins, like their plant counterparts, are associated with immunity or disease development. High Mobility Group Box protein (HMGB) and Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) were identified as SABPs and play important roles in disease responses in both plants and humans.

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108


Development of techniques for practical applications of multifunctional microorganism in agriculture

Jun-Kyung Park1, Sun-Il Seo1, JaeKyeong Song2, Chul-Won Lee3, Yong-Hak Kim4, Pyoung Il Kim1*

1Center for Industrialization of Agricultural and Livestock Microorganisms, Jeongeup, Republic of Korea, 2Agricultural Microbiology Division, National Institute of Agricultural Sciences, RDA, Wanju, Republic of Korea,

3Department of Chemistry, Chonnam National University, Gwangju, Republic of Korea, 4Department of Microbiology, Daegu Catholic University School of Medicine, Daegu, Republic of Korea

The complete genome sequence of Bacillus velezensis GH1-13 revealed that it possesses a single 4,071,980-bp circular chromosome with 46.2% GC-content, and we also identified a unique plasmid of 71,628-bp with 31.7% GC-content. The genome was found to contain various enzyme-encoding operons, including indole-3-acetic acid (IAA) biosynthesis proteins, 2,3-butanediol dehydrogenase, various non-ribosomal peptide synthetases, and several polyketide synthases. The lipopeptides, including bacillomycin, fengycin and surfactin, were detected by mass spectrometry and NMR. The optimal carbon and nitrogen sources for mass cultivation were determined by glucose and soy bean flour, respectively. The results showed that 7.5×109 cells/mL, 6.8×109 endospore cells/mL and sporulation yield of 90% after 30 h cultivation in 500 L submerged fermenter at 37°C, pH 7.0. Cells and cell-free supernatant of GH1-13 strain exhibited the potent antifungal activity against phytopathogenic fungi. It was also confirmed that indole-3-acetic acid (IAA) production of GH1-13 strain was greatly increased by addition of 0.3% tryptophan. The microbial formulations of GH1-13 strain were performed into liquid, powder, granule and pellet types. Plant growth promoting of four formulation types were examined to pepper and tomato plants, and biocontrol effect of liquid and powder types was also tested against Colletotrichum gloeosporioides causing pepper anthracnose. Microbial agents of GH1-13 were applied in pepper and potato plant, and resulted in productivity enhancement of 27% and 18%, respectively.

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SymposiaS1

Biochemistry · Molecular Biology

S2Environmental Sciences

S3Natural Products · Bioactive Materials · Biomedical Sciences

S4Food Sciences

S5Applied Microbiology

S6Agro-Bio Genome Editing

S7Forensic Science

S8Pesticide Positive List System

110



Kyung-Ha Lee, Seong Hee Bhoo*

Graduate School of Biotechnology, KyungHee University, Yong-in 17104, Republic of Korea

Protein purification is an indispensable procedure in most of biology, biochemistry and molecular biology fields. Purification of target proteins with high quality and purity is generally difficult. Several tag systems have been established for this purpose of protein purification. Small peptide epitope tag and their affinity purification systems are excellent for protein purification because they have high affinity and specificity for acquiring the target proteins. Therefore an excellent tag should have high affinity and specificity for better purification. However, not all peptide based epitope tag systems meet these requirements. For example, the purification His6-tagged proteins using zinc ion chelate affinity resin often results in the non-specific co-purification of zinc ion binding proteins in the starting total proteins. Furthermore we often meet non-specific binding of monoclonal antibodies to endogenous proteins in certain cell types, even when using the most popular commercial tag systems such as Flag tag and Myc tag systems. In this case of monoclonal antibody based protein purification, antibodies with high affinity and specificity are critical requirement for the best protein purification. Most of antibodies have KD values in the low micromolar (10–6) to nanomolar (10–9) range. Even commercial tags such as HA, flag and myc, in which these tag are known as high affinities, have KD values of 4.5 nM, 6.5 nM and 80 nM respectably. These tags are commonly used worldwide for protein purification but not enough for the best protein purification. Here we developed a novel affinity tag based protein purification designated 2B8 tag system. 2B8 tag is consisted of 9 amino acids (RDPLPFFPP) which has an extremely low KD value of picomolar (10–12) range for 2B8 antibody. Since lower KD value means higher affinity, 2B8 tag system will be an outstanding protein purification system even more 2B8 tag is a highly specific to the antibody.

S1-1


Symposia

Symposia

Molecular and Functional Study of Insect Odorant Receptor

Young Kun Shim1,2, Sanung Eom1, Je Jun Oh1,2, Junho Lee1*

1Department of Biotechnology, Chonnam National University, Gwangju, Republic of Korea, 2Microzyme Corporation, Damyang Samanli 234, Jeonmam, Republic of Korea

The olfactory nervous system recognizes and distinguishes many different chemicals in the general living environment. Insects have evolved a group of odorant-gated ion channels composed of highly-developed olfactory receptors capable of distinguishing and distinguishing between various chemicals with symbolic or evasive specificities. Recently, aphid genomes related to olfaction, including olfactory receptors and proteins, have been identified and olfactory receptors have been reported that are differentially differentiated from Drosophila. The genome of the olfactory receptor has a very conservative sequence and a systematic signaling system. A representative receptor, odorant-gated ion channels comprised of a highly conserved co-receptor (Orco) has a homotetramer channel structure with four subunits arranged symmetrically around the central hole. It has a very similar structure to the 7-transmembrane receptor present in the human body and has a very similar structural form and gating mechanism to receptors of neurotransmitters.

In this study, whole cell voltage clamp recording was performed with cell expression system of OR85b gene, which is a subtype of olfactory neuro-receptor isolated from Drosophila. After the successful expression of this receptor, microbial culture extract of microorganism, a harmful insect inducer, was used to investigate whether olfactory receptor activity was regulated. The activity of the receptor was confirmed in the recording media diluted 10,000 times with the microbial culture extract.

Therefore, it is possible to identify attractant or repellent substance using the olfactory receptor activity regulating system of insects. Through this study, MZ01 shows the attracting phenomenon by activating insect receptor OR85b, The results of the scientific analysis of the performance of the extracts are presented.

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112


Elucidating the shared and unique responses of Arabidopsis thaliana to multiple environmental stresses

using meta-analysis approach

Ashutosh Singh*

Translational Bioinformatics and Computational Genomics Lab, Department of Life Sciences, Shiv Nadar University, Greater Noida, UP, India

BackgroundAbiotic stress severely affects both physical and biochemical properties of plant cells, which then eventually alter survival

and productivity. Abiotic stresses are one of the major causes of meagre plant growth and reduced crop yields globally. In most of the plant species, >50% growth reduction was observed due to abiotic stress. Since, the stress adaptation mechanisms are largely unknown, elucidating these tolerance mechanisms is essential to accelerate plant adaptability to natural field conditions in order to enhance their growth and yield. A huge amount of transcriptomic data is available for plants exposed to various abiotic stresses. Comparison of the transcriptomic data of plants exposed to individual and combined stresses may explain the molecular mechanisms behind the cross-talk between stresses. Additionally, comparison of molecular profiles of an organism under different stresses would make it possible to identify the conserved stress mechanisms. Gene co-expression networks study is becoming increasingly popular as one of the approaches to identify sets of interacting genes.

Arabidopsis thaliana is a well-studied model plant organism with extensive biological knowledge base and resources including complete genome sequence and the highest number of microarray studies. In the present study, a comprehensive meta-analysis using RankProd method was performed on A. thaliana microarray-based transcriptomic dataset for drought and cold stress. Further, co-expression network analysis was done using WGCNA R package.

ResultsFrom 29 series, 6350 and 7210 differentially expressed probes, mapped to 6120 and 7079 gene models in A. thaliana

were identified with PFP (percentage of false prediction) ≤ 0.01, under drought and cold stress respectively. Here, we report, 41% and 31% genes unique to drought and cold stress respectively. DEGs common to both the stresses were found to be 2890. Most of the genes showed conserved expression pattern (72% or 2083) with 1084 up-regulated and 999 down-regulated in both drought and cold stresses. Hence, molecular profiles of common DEGs suggests that common molecular pathways are altered in a similar manner in response to both the stresses. Further, gene ontology profiles of DEGs in cold and drought stresses were statistically compared to assess the biological similarity and differences between the two DEGs list. We were able to identify 142 common significant gene ontology terms such as, ‘photosynthesis’, ‘respiratory burst’, ‘response to hormone’, ‘signal transduction’, ‘metabolic process’, ‘response to water deprivation’ etc.

The co-expression network analysis clustered the degs of drought and cold stress into 21 and 16 modules respectively. The module preservation statistical analysis showed that eight cold modules have well-defined drought counterparts (summary Z-score >10) and two cold modules: Pink and Purple have moderate preservation (10 < Z-score > 5 and higher

S1-3


Symposia

Symposia

preservation median rank). The preserved modules genes were found to associate with biological processes: photosynthesis, stress acclimation process, response to abiotic stress and metabolic process, which generally altered during stress. The differential consensus module eigengene network analysis also advocated the similar findings and also suggested existence of a relationship among photosynthesis and defense response as well as relation between abiotic stress responsive and translation related genes under abiotic stress condition.

ConclusionThis analysis was able to identify DEGs which include- DEGs already reported by individual studies and additionally,

new DEGs which were overpass by individual studies. Thus, this approach magnifies the strength and sensitivity in the identification of vital stress response genes which may be overlooked by individual studies.

The comparative analysis of differential expression analysis and gene ontology enrichment of the two stresses revealed the existence of shared and unique components between cold and drought stress. It was found that several transcription factor families common in both the stresses regulates several common stress-responsive genes adhering to ABA-dependent pathway. The shared stress-responsive genes were found to be involved in ROS scavenging, stomatal movement etc. This helps the plant to reclaim the homeostatic state which was disturbed under the influence of both the stresses.

Gene co-expression network analysis also supported the findings of meta-expression analysis by revealing the existence of highly inter-correlated stress-specific and consensus modules with specific profiles of expression under drought and cold stress respectively. Altogether, the result from our study gives information about the common and unique biological and molecular behavior of the plant in response to various abiotic stresses which can be utilized for multiple stress response engineering.

114


Hormetic effect of veterinary antibiotics on the enzyme activity of soil planted with Pisum sativum L.

Reep Pandi Tasho, Jae Young Cho*

Department of Bioenvironment Chemistry, Chonbuk National University, Jeonju, Republic of Korea

Microbial communities are important for the ecosystem, whether for direct interactions with the plants or for nutrient and organic matter recycling. In this study, the hormetic effect of veterinary antibiotics- kanamycin (KA), sulfamethazine (SA), and tetracycline (TC) on soil microbial biomass and enzyme activity was examined. Soil microbial biomass and enzyme activity (dehydrogenase, urease, and alkaline phosphatase) were assessed under two different experimental conditions: A) Planted with Pisum sativum L. B) Unplanted. The role of pea plants in alleviating antibiotic toxicity was evident from the study. KA and TC antibiotics strongly adsorbed to soil components and were less bioavailable than SA. As a result, the test parameters were susceptible to increasing concentrations of SA. Soil microbial biomass exhibited a biphasic response for KA and TC antibiotics. However, the increase (+16%) was not enough to be classified as hormesis. Soil enzyme activity, on the other hand, depicted a dose-dependent hormetic response for KA and TC antibiotics. This study shows that nominal concentrations of KA and TC antibiotics, in presence of pea plants, increase the tolerance or adaptability to veterinary antibiotics by significantly enhancing the function of soil enzymes rather than increasing microbial biomass.

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Symposia

Methane cycling under nitrogen-limited conditions: diazotrophy as a life-strategy of methanogens and methantrophs?

Sang Yoon Kim1*, Ju-Hee Lee1, Paul L.E. Bodelier2

1Department of Bio-environmental Sciences, Sunchon National University, Suncheon, Republic of Korea, 2Netherlands Institute of Ecology (NIOO-KNAW), Department of Microbial Ecology, Wageningen, the Netherlands

Wetlands are the single largest source of atmospheric methane. The large uncertainties in the predictions of atmospheric methane are partly due to lack of understanding of the regulating factors of the underlying microbial processes. Net methane emission is the balance of methane production by archaeal methanogens and the consumption by aerobic and anaerobic bacteria and archaea, all affected by the availability of nitrogen. However, so far studies focused on nitrogen excess in fertilization studies. We investigated the performance of methane cycling microbes under nitrogen-limiting conditions with a special focus on N2 fixation. To create nitrogen limitation we used a planted (Glyceria maxima) microcosms system with separated root- and bulk soil compartments filled with sediment from an agricultural ditch. Actively N2 fixing methanogens and methanotrophs were screened in situ as well as in vitro by mRNA based assessment of nifH genes as well as by using stable isotopes in combination with fluorescent in situ hybridization and Nano scale secondary ion-beam mass spectrometry (NanoSIMS) of extracted cells. Methane emission was significantly lower in the absence of fertilizer. Both, growth and activity of methanogens and methanotrophs were reduced by limited by nitrogen availability. NanoSIMS of individual cells indicated that methanogens as well as methanotrophs are actively fixing N2 but at a very low level. Our results suggest that nitrogen availability is a strong regulating factor of methane emission from agricultural as well as natural wetlands and should be incorporated in methane emission models.

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116


Biochar as Catalytic Materials for Biorefinery Applications

Jechan Lee*

Department of Environmental and Safety Engineering, Ajou University, Suwon 16499, Republic of Korea

Biochar is a carbon-rich material, made by pyrolysis of biomass feedstocks. While biochar is widely used for removal of contaminates from soil and water, its applications beyond the soil and water remediation are relatively rarely studied. In this presentation, it will be introduced and discussed how to make biochar that can be used as heterogeneous catalysts and their applications to biorefinery, including biodiesel production, syngas production, tar removal, etc.

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Symposia

Imaging of β-amyloid plaques by near-infrared fluorescent probe in Alzheimer’s disease

Yong Dae Park*, Sungbeom Lee, Hyoung-Woo Bai, Byung Yeoup Chung, Seung Sik Lee

Research Division for Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 580-185, Republic of Korea

Amyloid depositions in brain are the main hallmarks of Alzheimer’s disease (AD). Since they are believed to precede clinical symptoms by several years, imaging of such fibrillary aggregates offers an opportunity of the early detection of AD and will be useful for identifying new therapeutic drugs and monitoring the treatment results as a surrogate marker. In this context, radionuclide or NIRF imaging has been proposed as a promising and noninvasive method to visualize ß-amyloid (Aβ) plaques in vivo because of its acceptable depth of penetration and minimal degree of tissue damage. Here, we describe the synthesis and characterization of the fluorescent curcumin derivative 5b, which readily penetrates the intact blood-brain barrier (BBB) and binds to Aβ plaques. Using NIRF imaging, we demonstrated the specific interaction of 5b with Aβ plaques in 5X FAD transgenic mice in vivo, as confirmed by the dissection analysis of brain slices. Semi-quantitative analysis revealed that an intense fluorescence signal was detected in the brain, and the significant binding of 5b was observed for 5X FAD transgenic mice aged 15 months. Thus, the curcumin derivative 5b is a promising noninvasive NIRF imaging probe to visualize amyloid plaques and evaluate the effects of potential AD drugs in vivo.

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118


Total Synthesis of Antibiotic Disciformycin A and B1

Yonghoon Kwon, Saskia Schulthoff, Quang Minh Dao, Conny Wirtz, Alois Fürstner*

Max-Planck-Institute for Kohlenforschung, Mülheim an der Ruhr, Germany

The development of ring closing alkyne metathesis (RCAM) followed by stereoselective reduction of the resulting alkyne has rendered opportunities to access Z- or E-alkenes.2 Beyond the selective formation of (Z)- or (E)-disubstituted alkenes, we recently demonstrated that a macrolide having a trisubstituted alkene with well-defined stereochemistry can be prepared by a RCAM/trans-selective hydrostannation sequence. This approach is complementary to ring closing alkene metathesis (RCM) since (stereoselective) formation of trisubstituted alkenes by RCM is problematic. Testing this method in the total synthesis of a complex natural product is desirable to broaden the generality of the strategy. Disciformycins A and B, isolated from cultures of Pyxidicoccus fallax by the Müller group in 2014,3 were chosen as our targets as they exhibit considerable antibacterial activity against Gram-positive bacteria. This presentation will describe details of unforeseen synthetic challenges and our endeavors to resolve these problems met along the way.

References(1) Kwon, Y.; Schulthoff, S.; Dao, Q. M.; Wirtz, C.; Fürstner, A. Chem. Eur. J. 2018, 24, 109–114.(2) Fürstner, A. Angew. Chem. Int. Ed. 2013, 52, 2794–2819.(3) Surup, F.; Viehrig, K.; Mohr, K. I.; Herrmann, J.; Jansen, R.; Mülller, R. Angew. Chem. Int. Ed. 2014, 53, 13588–

13591.

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Symposia

Relation of environmental variation to skin biophysiological parameters of Korean females

Jihye Maeng1, Gaewon Nam1,2*

1Seowon Skin Research Center, Cheongju 28674, Republic of Korea, 2Departments of Biocosmetic Science, Seowon University, Cheongju 28674, Republic of Korea

Skin is the most organs on the surface of the body and is the organ where the phenotype of aging appears. Skin aging is an endogenous cause that progresses with time and exogenous causes caused by the external environment. Aging due to UV exposure is one of the causes of extrinsic aging, and UV directly or indirectly affects cells to promote senescence. It produces ROS (Reactive oxygen species) in the cell during the influence of UV. ROS affects DNA breakdown, oxidation of proteins and lipids in the cell, and carbonylated protein produced by protein oxidation in the stratum corneum of the skin is under investigation for skin aging.

The purpose of this study was to investigate the correlation between carbonylated protein (ROS) induced by oxidative stress induced by ROS and wrinkle improvement and elasticity improvement. In the in vitro test, the skin improvement test with the antioxidant-proven raw materials showed that the reduction of carbonylated protein, the improvement of skin wrinkles and the improvement of skin elasticity were found to be related to each other. This study confirmed the possibility of applying the carbonylated protein assay to the skin aging test.

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120


Studies on volatile analysis of various foods by different extraction techniques

Yoon-Yeol Lee, Jun-Young Lee, Hee-Don Choi, Hae Won Jang*

Korea Food Research Institute, Nonsaengmyeong-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do, Republic of Korea

Several existing analytical methods are available for the analysis of volatile compounds detected from various foods. Gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS) methods have employed several volatile extraction techniques including distillation, solvent extraction, purge-trap or dynamic headspace techniques. Especially, headspace techniques such as stir bar sorptive extraction (HS-SBSE) and solid phase microextraction (SPME), dynamic headspace (DHS) used mainly these days regarding a simple, rapid, and solvent free technique for the volatile extraction. Until the present study, HS-SBSE has shown to provide the higher recoveries than HS-SPME and has improved the analytical performance in terms of the elimination of interfering substances and low concentration capacity. In recent years, SPME Arrow technology has been developed as a new tool of the SPME system and it combines advantages of the SPME and the HS-SBSE. In spite of the use in larger sorbent phase volumes and longer diameter and length of the fiber, a thermal desorption unit is not required for the SPME Arrow. However, very little information on volatile analysis in various food samples using HS-SBSE and HS-SPME-Arrow is available today.

In the present study, volatile compounds from various foods were analyzed by using HS-SBSE-TDU-GC-MS and HS-SPME-Arrow-GC-MS equipped with a 60 m×0.25 mm i.d. (df=0.25 μm) DB-WAX bonded-phase fused-silica capillary column. HS-SBSE and HS-SPME-Arrow methods were optimized for detecting volatile compounds by following extraction conditions; adsorbent phase, extraction temperature, extraction time, and NaCl concentration, sample preparation before extraction. These newly introduced techniques were compared for the utility with SPME method and showed the better sensitivity and reproducibly than HS-SPME. These results support the use of HS-SBSE and SPME Arrow for volatile analysis from foods.

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Symposia

Anti-obesity effect of green bean fermented by lactic acid bacteria

Myoung Nim Shin1*, Yong Seob Jeong2

1Department of Bioenvironmental Chemistry, Chonbuk National University, Jeonju 567, Republic of Korea, 2Department of Food Science and Technology, Chonbuk National University, Jeonju 567, Republic of Korea

The aim of this study was to ensure that materials with high quality bio-antiobesity can be developed by fermenting coffee green beans with various physiological activity, using lactic acid bacteria. In this study on the evaluation of the anti-obesity, using the R. toruloides model, the extract from fermented green beans showed increase of physiological activity materials and neutral lipid. Also, on 3T3-L1 preadipocyte experiment, inhibition on the lipid differentiation and accumulation was found. These results suggest that coffee green bean by lactobacillus-mediated fermentation and with the increase of these compounds may be increased anti-obesity effect.

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122


Database on flavonoids and phenolic acids in Korean agro-food resources using mass technology

Heon-Woong Kim, Min-Ki Lee, Seon-Hye Lee, Gelila Asamenew, Suji Lee, Sang Hoon Lee, Seon Mi Yoo, Jung-Bong Kim*

National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea

Flavonoids and phenolic acids are the most important group in secondary plant metabolites and generally produced as defense mechanisms against pathogens and disease organisms. These compounds have been reported to play an important role in the preventive aspects in human disease. Therefore, we have developed a comprehensive database (DB) on flavonoids and phenolic acids contained in the main foods consumed by the diet. In order to construct DB from agro-foods, the LC-MS condition for the flavonoids and phenolic acid derivatives were optimized and the identification of these structures was completed through interpretation of library and positive mass fragmentation. Based on mass technology (QToF-MS), flavonoid (1,950 pages, 268 agro-foods) and phenolic acid (1,130 pages, 308 agro-foods) DB was composed of their contents, chemical library and chromatogram/mass spectrometric data including 1,684 flavonoids and 1,163 phenolic acids, respectively. Furthermore, by using this information, the functional ingredient search and characterized maps were available for user through building web service system (http://koreanfood.rda.go.kr). Our research can be used as the basic data for the study and application of functional ingredients to contribute to food industry development, understanding of metabolic pathways, and discovery of biological activity as a national food functional DB.

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Symposia

Unraveling the DNA damage response in the radiation-resistant fungus, Cryptococcus neoformans

Kwang-Woo Jung1, Yeonseon Lee2, Doog-Hoon Yang2, Min-Kyu Kim1, Ho Seong Seo1, Eun Young Huh3, Soo Chan Lee3, Sangyong Lim1*, Yong-Sun Bahn2*

1Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup, Republic of Korea, 2Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Republic of Korea,

3South Texas Center for Emerging Infectious Diseases (STCEID), Department of Biology, the University of Texas at San Antonio, San Antonio, Texas, USA

Although there are no natural environments under intense radiation, some living organisms have been found to show high radiation resistance. The basidiomycetous fungus Cryptococcus neoformans has been known to be highly radiation resistant and has been often found in fatal radioactive environments. To elucidate the mechanisms underlying the radiation-resistance phenotype of C. neoformans, we identified genes affected by γ-radiation through genome-wide transcriptome analysis and characterized their functions. We found that genes involved in DNA damage repair systems were upregulated whereas genes involved in the ergosterol biosynthesis were downregulated post γ-radiation exposure. Most importantly, we discovered a number of novel C. neoformans genes, the expression of which was modulated by γ-radiation exposure, and their deletion rendered cells susceptible to γ-radiation exposure, as well as DNA damage insults. Among these genes, we found that a unique transcription factor containing the basic leucine zipper domain, named Bdr1 (a bZIP TF for DNA damage response 1), served as a regulator of the γ-radiation resistance of C. neoformans by controlling expression of DNA repair genes, and its expression was regulated by the evolutionarily conserved DNA damage response protein kinase Rad53. Furthermore, we demonstrated that Rad53 was required for DNA damage response and was phosphorylated by both PI3K-like kinases Tel1 and Mec1 in response to DNA damage. Transcriptome analysis revealed that expression levels of genes involved in the DNA repair and DNA replication were controlled by Rad53 in response to γ-radiation. Moreover, Chk1, which is another effector kinase like Rad53 in the budding yeast, exhibit redundant and distinct roles in the DNA damage stress. Taken together, the current transcriptome and functional analyses could shed light on understanding γ-radiation response of C. neoformans.

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124


Regulation of fungal development and pathogenesis through histone acetylation/deacetylation in the rice blast fungus

Jaejoon Lee, Jongjoon Lee, Taehyun Kim, Gnanendra Shanmugam, Junhyun Jeon*

Department of Biotechnology, College of Life and Applied Sciences, Yeungnam University, Gyeongsan, Gyeongbuk 38541, Republic of Korea

Fungal pathogens have huge impact on health and economic wellbeing of human by causing life-threatening mycoses in immune-compromised patients or by destroying crop plants. A key determinant of fungal pathogenesis is their ability to undergo developmental changes in response to host or environmental factors. Here we set out to investigate contribution of one of the most important epigenetic modifications, histone acetylation/deacetylation to this morphogenetic process, using a model plant pathogenic fungus, Magnaporthe oryzae. For functional and comparative analysis of histone modifications, a web-based database (dbHiMo) was constructed first to archive and analyze histone modifying enzymes from eukaryotic species whose genome sequences are available. Based on the database entries, we carried out functional analysis of genes encoding histone acetyltransferases (HAT) and deacetylases (HDAC). Gene deletion or silencing approaches showed that disruption of histone acetylation status generally leads to defect in vegetative growth and asexual sporulation. RNA-seq analysis on one of the HAT mutant (∆Mosas3) indicated that such growth defect is related to down-regulation of a whole array of genes involved in nitrogen and carbon metabolisms. Differentially expressed genes in the mutant showed significant overlap with genes that are differentially expressed in ∆Mortt109 mutant, suggesting line between DNA damage response/repair and metabolic repression in epigenetic level. For HDAC, our data suggest that Class I HDACs have great impact on fungal biology. In particular, we revealed that MoHOS2 is required for asexual reproduction through stage-specific regulation of some of the conidiogenesis-related genes. Interestingly, in ∆Mohos2, it appears that reduced pathogenicity is attributed to mis-regulation of genes encoding effector proteins. Furthermore, we showed that lack of deacetylase activity alone is able to recapitulate most of phenotypic defect in ∆Mohos2. Taken together, our systematic analyses provide not only insights into implication of histone acetylation/deacetylation in fungal development and pathogenesis but also novel targets for new agrochemicals against fungal pathogens of plants.

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Symposia

Therapeutic effect of extracellular matrix degradation enzyme delivered by combination engineered bacteria with anticancer drugs

Jam-Eon Park, Seung-Hyeon Choi, Ju Huck Lee, Seung-Hwan Park*

Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup 56212, Jeollabuk-do, Republic of Korea

To overcome limitations associated with bacteria-mediated cancer therapy, the engineered bacteria that encoded therapeutic drug have been reported. Several attenuated bacteria such as Salmonella typhimurium could target tumor tissue and regress the tumor mass. However, engineered bacteria have some hurdle to apply the clinical applications. We designed the combination therapy with bacteria and anti-cancer drugs. Extracellular matrix (ECM) of tumor tissue is one of targets for tumor therapy since the growth of tumor cells and tissues depends on its formation. Hyaluronic acid (HA) is major polysaccharide component of ECM and increases the interstitial fluid pressure (IFP) within the tumor tissue by absorbing significant amounts of water, resulting in an inhibition of anti-cancer drugs diffusion to tumor tissue. In this study, we assessed a possibility that ECM breakdown would increase the activity of tumor therapeutic drugs. We constructed plasmid encoded highly expressing hyaluronidase (HAase) under the controlled constitutive promoter originated from Staphylococcus aureus. The transformed attenuated S. typhimurium ΔppGpp (SL-HAase) exhibited the enzyme activity that degraded HA in agar plates. The bacterial lysates showed enhanced enzyme activity over 16-fold compared to control bacteria. In order to measure in vivo degradation of HA in tumor tissue, we injected SL-HAase into 4T1 and PC3 xenograft models intraveneously resulting in observation of significant decrease of ECM in both 4T1 and PC3 tumor tissue after 5 days compare to control group. To assess the combination therapy of an engineered bacteria with a chemotherapeutic drug, we injected ECM degrading bacteria and then doxorubicin every 2 days (a half of dose, 5 mg/Kg) in 4T1 and PC3 xenograft models, resulted in inhibition of tumor growth compared to control group. The ability of ECM degrading bacteria to enhance chemotherapy efficacy is likely due to increased drug perfusion by reduced IFP.

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126


Trait modifications using a protoplast CRISPR/Cas9 system in horticultural crops: review and progress

Geung-Joo Lee*, Luhua Tu, Saminathan Subburaj, Ka Yeon Lee, Yong Sam Jeon, Kwang Soo Park

Department of Horticultural Science, Chungnam National University, Daejeon 34134, Republic of Korea

Crop improvement through various breeding tools has contributed to sustainable agriculture, and been practiced to enhance yield and quality of biomaterials for human beings. Conventional breeding technologies, have been greatly used to enhance crop production. Recently, a targeted genome editing (TGE) using engineered nucleases such as clustered regularly interspaced short palindromic repeat (CRISPR) nucleases has been applied to improve the traits of plants successfully. TGE has emerged as a novel plant-breeding tool that represents an alternative approach to classical breeding, but with higher mutagenic efficiency. The CRISPR/Cas9 system was originally developed based on a defense system used by prokaryotes against foreign pathogens. The CRISPR/Cas9 system consists of RNA-guided engineered nucleases (RGNs) that recognize their cognate nucleotide sequences in target genes through complementary base pairing. CRISPR/Cas9-mediated genome editing through a direct delivery of pre-complexed purified CAS9 protein along with in vitro-transcribed sgRNA can be achieved by adopting a protoplast culture, with advantages of no requirement of codon optimization or expression promoter, and DNA-free editing for bypassing GMO regulations. Research groups have recently reported target-directed genome editing using protoplast transformation and whole-plant regeneration technology in some horticultural crops species including lettuce, tobacco, apple and potato. In a continuation of previously established protocol for gene knock-out in petunia protoplasts via Cas9-RNP delivery, we have successfully performed site-specific mutagenesis in petunia to modify flower color modifications. From the recent results, it was found that orthologous or paralogous genes, which occur widely in plant speciation, should be investigated when the CRISPR-Cas system is applied to target-specific gene editing to achieve proper target mutagenesis in plants.

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Symposia

Optimization of meristem maturation and shoot growth in tomato

Jung Heo1, Ryza A. Priatama1,2, Eunsong Lee1, Sukgui Oh3, Jong Hyang Bae3, Soon Ju Park1*

1Department of Biological Science and Institute for Basic Science, Wonkwang University, 460 Iksandae-ro, Iksan, Jeonbuk, Republic of Korea, 2Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research

Center (PMBBRC), Gyeongsang National University, Jinju, Republic of Korea, 3Department of Horticulture Industry, Wonkwang University, Iksan 54538, Republic of Korea

Sympodial growth and flowering time are two major traits for high yield tomato, regulated by molecular maturation of shoot apical meristem. Here we show that tomato productivity can be fine-tuned and optimized by exploiting mutations controlling flowering time pathway and shoot growth pathway. First, we show the mutants showing relatively weak late flowering, among selected flowering time mutants, produced more yield compared with the determinate tomato. A new sp weak mutant allele, sp-5732, from 242 Core Collection produced more sympodial shoots and improved tomato fruit yield up to 42% in the field. Second, we discovered IL3-2-2, overexpressing tomato florigen, could optimize crop productivity between leaf and inflorescence production among the variants bearing succesively two or three leaves sympodial index of indeterminate tomato. Third, we also bred compact tomato varieties using sler and sod mutants, which allow tomato cultivation under restricted space conditions such as growth chamber in building. Alltogether, these variants support new resources for tomato breeding and the function of genes could be used as multiple genetic targets to overcome background issue on breeding process with advances in biotechnology.

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128


Identification of Genes Involved in Trichome Development and Insect Resistance in Tomato

Jae-In Chun, Won-Ki Hong, Heejin Kim, Jin-Ho Kang*

Graduate School of International Agricultural Technology and Crop Biotechnology Institute, Seoul National University, Pyeongchang 25354, Republic of Korea

Trichomes are specialized epidermal structures that protect plants from abiotic and biotic stresses. In depth knowledge of the molecular mechanisms that control trichome development in Arabidopsis, which produces unicellular nonglandular trichomes, has provided significant insight into the genetic basis of variation in trichome habit. Solanaceous plants including tomato produce several different types of multicellular nonglandular and glandular trichomes on aerial tissues. In contrast to our understanding of unicellular nonglandular trichomes, much less is known about the development and ecological function of multicellular trichomes. Here, we report identification of several genes involved in trichome development and their roles in plant defense against insect attack in tomato.

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Symposia

Symposia

Forensic Chemistry: Review of Status and Needs

Jin Young Kim*

Forensic Genetics & Chemistry Division, Supreme Prosecutors’ Office, Seoul 06590, Republic of Korea

The term “forensic” is practically a synonym for “legal” or “related to courts”. Forensic Genetics & Chemistry Division in Supreme Prosecutors’ Office of Korea is divided into two main areas of scientific investigation: forensic chemistry and DNA & biology. Forensic chemistry deals with application of chemical principles for the solution of problems that may arise in conjunction with serving justice. It embraces wide and diversified fields, which include not only forensic toxicology but also the analysis of any material that may have presence in the course of legal proceedings. The Forensic Chemistry section conducts analysis on varieties of physical evidences relating to criminal cases such as illegal medicines, poisons, adulterated foods, narcotics and hallucinogenics as well as harmful chemicals in various biological samples and seized materials. Our section has maintained ISO/IEC 17025 accreditation which is the most important international standard for testing and calibration laboratories. In addition, the lab conducts research on noble analytical techniques for new psychoactive substances, drug impurity profiling as well as other crime scene evidences to cope with the rapid changing criminal environment. In this presentation, a brief summary of current status of forensic chemistry as well as recent legal challenges for handling crime scene evidences in the course of scientific investigation will be discussed.

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130


Identification of phytolaccosides in the biological samples from pokeweed intoxication patients using liquid

chromatography-tandem mass spectrometry

Sanggil Choe1, Suncheun Kim2*

1Drug and Toxicology Section, Seoul Institute, National Forensic Service, 08036, Republic of Korea, 2Fornesic Toxicology & Chemistry Division, Daejeon Institute, National Forensic Service, 34054, Republic of Korea

Many plants in the Phytolaccaceae are distributed throughout tropical and subtropical area. About 35 species was included in genus Phytolacca, and they are common in America, South-Africa and South-Asia. Among the Phytolacca-species, P. americana L. (American pokeweed) and P. esculenta VAN HOUTTE (Chinese pokeweed) are two representative species. While P. americana is native to the temperate regions of North America and it propagated over the whole world, P. esculenta is native to China and its occurrence has been reported from many European country.

Pokeweed have toxic compounds in fruits, roots, leaves and stems, and it was reported that the toxic substances were the most abundant in roots and the amount of toxic compounds were not different between the two species. Pokeweed are toxic to cattle, horses and man. It was reported that their toxicity in cow included severe gastro-intestinal irritations, spams, purging, convulsions and death by paralysis of the respiratory organs, and abdominal pain, nausea, vomit, diarrhea, visual and auditory hallucination, and mental change were reported from the patients who had eaten pokeweed roots raw.

Pokeweed produces various types of chemical compounds. From the roots of P. americana, four types of lectins and lignanes were isolated from seeds, and betalain pigments from berries. The toxic compounds of pokeweed are triterpenoid saponins. As saponins, phytolaccoside A, B, D, E, G were isolated from P. americana, and esculentoside H, J, L, K, M, I and N were isolated from P. esculenta. With saponins, their aglycon phytolaccagenin, phytolaccagenic acid, esculentic acid and jaligonic acid were also isolated from P. americana and P. esculenta.

Two persons took plant roots misidentification and transferred to emergency room with vomit and abdominal pain. Blood and gastric contents from irrigation were collected from the patient 1, and blood and urine were collected from the patient 2. The biological samples and the roots were analyzed with liquid chromatography-tandem mass spectrometry (LC-MS/MS) to identify toxic substances. From the blood, gastric contents, and the ingested roots, esculentoside A, B, C, and H were identified, and very small amounts of esculentoside A, B, H were identified from urine. The applied analytical method was validated for the parameters such as linearity, limit of detection, precision, accuracy, matrix effect, recovery, and process efficiency.

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Symposia

Military forensic science and Applied Biological Chemistry

Choong-Sik Lee*

Division of Toxicology and Chemistry, Criminal Investigation Command, Ministry of National Defense, Seoul 04383, Republic of Korea

Scientific Investigation Laboratory (SIL) of Criminal Investigation Command (CIC), Ministry of National Defense (MND) in Korea pursues substantial truth through scientific experiments on military-related crimes and accidents and provides educational supports regarding scientific investigation. As the only forensic examination agency in the Korean military, SIL is internationally accredited as forensic institution from KOLAS (Korea Laboratories Accreditation Scheme) and As methods of committing crime are becoming more diverse, we are making efforts to develop new examination methods.

The forensic examination of natural science is the process that reveal the cause of death and accident and the offender by scientific experiments and thinking for the fields of chemistry, biology, physics, engineering and medical science and so on. Finding objective and scientific evidence in the crime scene is an important factor in making reasonable decisions under the judicial system. SIL of MND works for the examination and research of forensic science about the field of autopsy, chemistry, toxicology, DNA, document, fingerprint, image, digital, firearm, toolmark and criminal psychology in the area of military. Particularly, in the applied biological chemistry it is performed that the cause of death and drug-facilitated crime including sexual assault are revealed by the analysis of abuse drugs and toxic chemical and a offender is identified by DNA analysis. Also, the environmental pollutants such as petroleums, asbestos, lead, PAHs and so on were examined. If there is a difference from other forensic institutions, SIL is in charge of firearms/explosives analysis and DNA comparison test for the remains in the Korean War. Particularly, On March 2010, in the sinking of Korean navy ship, named as Cheonan, by the attack of North Korea, we participated in the part of forensic science with many authorized institutions and identified the high explosives by UPLC-Q/TOF. And also, on August 2015, in the DMZ landmine provocation 2 sergeants were amputated. Our team identified TNT (with other evidences as the fragments of landmine body) and verified the provocation by North Korea.

For the research, recently, the classification of petroleum or soil evidences were statistically interpreted by multivariate analysis such as principle component analysis, linear discrimnant analysis and support vector machine. The comparison of IR frequencies between ab initio calculation and experiment for the understanding and member education was researched by the quantum chemistry. The formation of anion adduct and properties of explosives in gas-phase are studied.

SIL of MND are doing our best for defense security, safety of people and truth-seeking. When the professional scientists in various fields should assist and volunteer in the military forensic science, SIL will be built as a more specialized forensic institution.

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Quantitative Tandem Mass Spectrometry for Simultaneous Analysis of Pesticide Multiresidues in Human Serum and Urine

Yongho Shin1, Jonghwa Lee2, Jiho Lee2, Junghak Lee2, Eunyoung Park2, Hye Suk Lee1, Jeong-Han Kim2*

1Drug Metabolism and Bioanalysis Laboratory, College of Pharmacy, The Catholic University of Korea, Bucheon-si, Gyeonggi-do 14662, Republic of Korea,

2Pesticide Chemistry and Toxicology Laboratory, Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea

Determination of multiresidual pesticides in a biological sample is essential for an immediate decision and response related to various pesticide intoxication. Serum and urine samples are very useful to detect pesticides within 2-3 days after acute poisoning from victims or patients. In this study, a rapid and simultaneous analytical method for pesticide multiresidues (more than 300 analytes) in serum and urine was established using liquid and gas chromatography-triple quadrupole mass spectrometer (LC-MS/MS and GC-MS/MS). The positive/negative switching electrospray ionization (ESI) mode and scheduled multiple reaction monitoring (MRM) were used to perform high-throughput pesticide detection within 15 minutes. Tiny volumes of serum or urine (100 μL) were used for sample preparation considering a realistic situation where a lot of urine cannot be collected from a pesticide poisoning victim or patient. For the optimization of sample preparation, three versions of QuEChERS (Quick, Easy, Cheep, Effective, Rugged, and Safe) procedures were scaled-down and compared, and the procedure using magnesium sulfate and sodium chloride without non-buffer reagents as well as dSPE clean-up was selected. The limit of quantitation (LOQ) in this method was 10 ng/mL for most of the target pesticides, sufficiently low to detect pesticides in applications. To evaluate the reliability of the established method, validation such as linearity, accuracy/precision and recovery test were conducted and the results satisfied the reasonably established criteria. The established bioanalytical methods are sufficient for application to biomonitoring in agricultural exposures and applicable in the forensic and clinic.

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Symposia

A Key to the Success of PLS Implementation: Authorative Registration of Pesticides Using Minor Crops

Su-Myeong Hong*, Hee-Dong Lee, Sang-Bum Lee

Department of Agro-material Assessment, National Institute of Agricultural Sciences, RDA, Wanju 55365, Republic of Korea

Rural Development Administration (RDA) has registered 314 pesticides by 2017, enabling them to use 1,223 pest control programs for farmers to provide safe agricultural products to the people and to provide suitable pesticides for farmers as a result of the authorative registration of pesticides since 1998. Authorative registration of pesticides using minor crops can be divided into three stages of projects before 2012, 2013~2017 and after 2018 project, and it is important to prepare for positive list system(PLS) of pesticides be implemented in 2019.

Minor crops introduced and cultivated by farmers for income increase are required to register pesticides due to the occurrence of pests in the crops two to three years after introduction. The registration tests of unregistered pesticides is performed by the Rural Development Administration (RDA) after 2~3 years.

It is difficult to satisfy the demand of various pesticides which can be used in the field by the method of registering one crop after one conventional crop test. In order to solve this problem, the RDA introduced a group registration system in 2017 and applied the results of one crop test to various crops.

In 2018, 248 activity and phytotoxic tests and 949 crop residue tests were conducted in the first year of large-scale authorative registration testing. As a result, 1,670 pesticides were newly registered and 4,441 pesticide safe use guidelines were established provisionally. Finally 7,018 pesticides which will be officially registered until 2021 were applied for use expansion registration with such provisional safe use guidelines.

In 2019, it will be to carry out 246 activity and phytotoxic tests and 880 crop residue tests to register 1,853 pesticides.

S8-1

134


Pesticide MRL Setting and PLS Enforcement in Korea

Jin Sook Kim*

Residues and Contaminants Standard Division, Ministry of Food and Drug Safety, Osong Health Technology Administration 187, Republic of Korea

There are two types of pesticide MRLs setting in Korea. Domestic and Import tolerance (IT). Setting of domestic MRLs carried out when the standard is requested by the Rural Development Administration, and the MRLs of the imported agricultural products are established at the time of application for IT from the pesticide company or the food company. Both are recognized as MRLs in Korea and applied equally to domestic and imported agricultural products. To propose MRLs, the residual data of each field trials are evaluated according to CODEX guide line or EU evaluation manual. The OECD calculator value is proposed as a MRL and considered to be in harmony with the codex MRL if the submitted data is identical to the data reviewed by the codex. MRL of countries that have performed field trials or applied for IT, and MRL for major exporting countries also can be considered. If the proposed MRL is applied and there is no concern for the chronic risk assessment of Korean, the proposed MRL will be reviewed and confirmed at the Expert Review Meeting and the Food Hygiene Deliberation Committee.

In Korea, a new pesticide management system, Positive List System (PLS), has begun. The first stage was started on 31, December 2016 for tree nuts, oilseeds and tropical fruits, and the second stage was implemented on 1, January 2019 for other crops. The purpose of introducing PLS is to protect the health of the people by preventing misuse and abuse of pesticides. The introduction of PLS is expected to increase the public confidence in agricultural products and improve competitiveness of agricultural products produced in Korea.

S8-2


Symposia

Symposia

Suggestion of Solutions to Problems Caused by Implementation of Positive List System

Se-Yeon Kwak, Jang-Eok Kim*


The improved living standards of people render a quality life, and the social favorability of well-being and safe food for a good health is also increasing day by day. Many Pesticides applied to crops for controlling insect pests, phytopathogens, and weeds can remain in the final agricultural products. Accordingly, in order to strengthen the safety management of agricultural products and to secure international competitiveness, Korea government agencies have introduced the ‘Positive List System (PLS)’ for the domestic and overseas imported agri-food products from January 2019. PLS is a system that administers unregistered pesticides for which no MRL is set to specific crop, at a uniform level of 0.01 mg/kg.

In contrast, during implementation of PLS, various concerns are being raised by the agricultural producers because the unpredictable pesticides can remain in the crop irrespective of the intentional farming activities such as soil residue pesticides, residues in rotational crops and drift of applied pesticides. Therefore, for the successful implementation of PLS, a strategy to minimize the damage of producers due to unpredictable pesticide contamination is needed.

The pesticide residues in the soil, which can occupy the largest portion by unpredictable pesticides, may be taken up by crops. This may pose a major risk in respect to the safety of these products for human consumption and economical damage related to PLS for producers. Many reports have shown that significant amounts of pesticide residues in agricultural soils can be taken up by plant roots, and transferred to edible parts, such as leaves and/or fruits. In particular, because pesticides residues absorbed from soil are mostly retained in the roots of the plant, root and tuber crops may be the most susceptible to pesticide residue contamination.

Today, I would like to present the results of the actual experiment data on uptake to crops of pesticide residues in the soil that may be affected by the implementation of the PLS system.

Keywords: Crop uptake, Pesticide residue, PLS, Soil management criteria

S8-3



GS1Biochemistry · Molecular Biology

GS2Environmental Sciences

GS3Natural Products · Bioactive Materials · Biomedical Sciences

GS4Food Sciences

138


Role of Cellular NAG-1/GDF15 in Tumorigenesis

Jaehak Lee, Ilju Kim, Seung Joon Baek*

Laboratory of Signal Transduction, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of Korea

Nonsteroidal Anti-inflammatory Drug Activated Gene-1 (NAG-1), also known as Growth Differentiation Factor 15 (GDF15), is a TGF-β superfamily protein which has multiple roles in diseases and a distinctive secretion pathway; synthesizes in cytoplasm, moves to the nucleus, translocates to cytoplasm, and secretes to the extracellular matrix. In cancer, NAG-1 seems to have both pro-cancer and anti-cancer activity. Considering its contradictory activity in cancer reported in the literature, our previous reports indicated that NAG-1 is translocated in different intercellular organs and exhibits different biological activity depending on its location. In this study, we further confirm that transfection of peptide corresponding to the NAG-1’s nuclear localization signal (NLS) blocks NAG-1’s translocation to the nucleus. We also found that the peptide was well-transfected in a time manner, demonstrating with high content screening system. Overall, our results indicate that NLS peptide derived from NAG-1’s sequence may have a potential tool for inhibiting NAG-1 secretion, thereby contributing to NAG-1’s role in anti-tumorigenesis. This work was also supported by the Research Institute for Veterinary Science, and BK21 PLUS Program for Creative Veterinary Science Research Center, Seoul National University, and by the National Research Foundation of Korea (NRF) grant funded by the Korea government (NRF-2018R1A2B2002923).

GS1-1


Graduate S

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Biogenesis of outer membrane vesicles derived from Escherichia coli small RNAs and their implications

Sohee Lee, Kwang-sun Kim*


Outer membrane vesicles (OMVs) produced by Gram-negative bacteria have been investigated during few decades. It is well known that their biological functions are either protecting or damaging bacteria from environment stresses. Furthermore, OMVs are utilized as vaccine or drug delivery platforms in terms of their efficient immune responses to host cells. Despite of the importance the biogenesis of OMVs are still remained elusive. At present, OMVs are released from outer membrane (OM) of bacteria through pinching off or bulging during bacterial growth. Additional models of OMV biogenesis have been suggested, the process is still poorly understood. Recently, non-coding RNAs (sRNAs) known to act as regulators of cellular functions by modulating gene expression have been suggested to be involved in the OMV production. Moreover, several sRNAs play critical roles of modulating gene expression by interacting with their specific target mRNAs as well as regulating outer membrane protein (OMPs). Therefore, the roles of sRNAs in OMV biogenesis needs to be investigated systematically. In this study, we performed the screening of sRNAs on the production of OMVs. Using sRNA-derived OMVs we performed proteomics analysis and profiles the protein components in OMVs. Furthermore, genes either up- or down-regulated in such OMVs were evaluated and suggested as effectors on OMV production. All our data suggest that sRNAs are hidden regulators to elucidate the cellular factors of OMV production. New information in this study will be utilized in the designing engineered OMVs as vaccine platforms.

GS1-2

140


Antioxidant Components and Activities of FRCE on H2O2-induced Oxidative Stress in HepG2 Cells

Sang Hyuk Lee, Ryong Kong, Jae Won Lee, Doo Jin Choi, Dae Young Lee, Geum-Soog Kim, Young-Seob Lee*

Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration (RDA), 92 Bisan-ro, Eumsung, Chungbuk 27709, Republic of Korea

Lactic acid production has received greater attention due to its considerable potential in biotechnological applications in a wide range of fields as well as the increasing need for new biomaterials, such as biodegradable, biocompatible, and polylactic products. Studies on the use of lactic acid bacteria as fermentation strains have been carried out, but studies on the physicochemical properties and functional properties of lactic fermented products using extracts have been rarely conducted. The aim of this study was to evaluate whether FRCE exerts beneficial effects against oxidative stress damage in HepG2 cells. In vitro antioxidant studies (DPPH, ABTS, and ORAC assays) jointly exhibited the potent antioxidant capacity of FRCE. Further study indicated that FRCE protected human liver HepG2 cells from H2O2-induced cytotoxicity by scavenging overproduced cellular ROS. In HepG2 cells, treatment of FRCE diminished H2O2-mediated oxidative stress and restored both the expression and the activity of certain antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and thioredoxin reductase (TRR). CAT, SOD and TRR activities were upregulated by treatment with the purified protein. CAT mRNA expression was significantly increased in HepG2 cells treated with FRCE. Therefore, the present study hypothesizes that the FRCE has the potential to protect the liver against oxidative stress-induced cell damage and should be considered as an antioxidant.

GS1-3


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resentation


Alternative splicing of thrombospondin gene in tumorigenesis

Yu Kyung Hong1, Chang Hwan Ryu2, Jun Sun Ryu2, Seung Joon Baek1*

1Laboratory of Signal Transduction, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of Korea,

2Department of Otolaryngology-Head and Neck Surgery, Center for Thyroid Cancer, Research Institute and Hospital, National Cancer Center, Goyang, Republic of Korea

Thyroid cancer is one of the most common cancer in worldwide and incidence of this cancer has been increased during the last decades. In addition, unnecessary surgeries are being carried out due to no specific biomarker for the diagnose. Therefore, identification of novel biomarkers should be considered for thyroid cancer diagnosis. Antibody arrays were performed using normal and tumor tissue of thyroid cancer patient and found several biomarkers that could be used in thyroid cancer patients. Among the candidate proteins chosen based on the antibody array, thrombospondin 1 (TSP-1) exhibited increased expression in tumor tissues, compared to adjacent normal tissues. Subsequently, TSP-1 transcripts were examined and found that a variant form (TSP-V) were expressed. Subsequent sequencing analysis revealed that intron retention (intron 12) occurs at a variant form of TSP-1. In addition, TSP-V increased when phytochemicals and NSAIDs treated in thyroid and breast cancer cell lines. Minigene system that expresses GFP will be used to test how anti-cancer compounds increases TSP-V form in cancer cells. Overall, our study indicated that TSP-1 produced splicing variant form, which possess potential anti-cancer activity and this variant form may provide a novel biomarker for the cancer prognosis. This work was also supported by the Research Institute for Veterinary Science, and BK21 PLUS Program for Creative Veterinary Science Research Center, Seoul National University, and by the National Research Foundation of Korea (NRF) grant funded by the Korea government (NRF-2018R1A2B2002923).

GS1-4

142


Morin hydrate inhibited dipeptidyl peptidase 1 and increased autophagic flux in response to ESBL-E.coli in HepG2 cell

line model of sepsis

Chanchal Sharma, Sun Chul Kang*


Modulation of CYP3A4 by flavonoids has been shown to inhibit liver damage by inflammation in mammalian cells. This study was conducted with HepG2 cell line to determine whether pre-treatment with morin hydrate (MH) can modulate E.coli ESBL (clinical isolate KBN10P03347 (MOI: 1:5)) mediated CYP imbalance and systematic infection. To this aim, initially 3D-QSAR was performed to determine the most appropriate prediction models for selective CYP inhibition. Next, molecular mechanism involved in the systematic infection was determined using cell death events as monitored by fluorescence microscopy. MH induces higher autophagy efflux by decreasing caspase 3 and Parp-1 in comparison to the E.coli ESBL treated groups. MH further inhibited the degradation of lysosome as evident by the expression of dipeptidyl peptidase 1 hence resulted in higher autophagy rate. The potential of MH treatment to reduce hepatic damage was also assessed by the levels of LDH, MDA and MPO. MH also markedly enhanced the antioxidant defence system by restoring the levels of superoxide dismutase-1 and glutathione peroxidase. These findings suggest that morin hydrate could be a promising therapeutic candidate for the treatment of ESBL-E.coli infection.

GS1-5


Graduate S

tudent P

resentation


Overexpression of ginseng-derived PgpPLAIIIβ altered cell growth patterns and reduced lignified fiber cells in Arabidopsis

and hybrid poplars

Jin Hoon Jang, Ok Ran Lee*

Department of Applied Plant Science, College of Agriculture and Life Science, Chonnam National University, Gwangju 61186, Republic of Korea

Lipid acyl hydrolase are diverse group of enzymes that hydrolyze the ester or amide bonds of fatty acid in plant lipids. Patatin-related phospholipase A (pPLAs) are major lipid acyl hydrolases that participate in signal transduction, membrane remodeling, and lipid metabolism. Based on Arabidopsis pPLAIII genes sequences, 11 members of pPLAIII genes in ginseng and 6 putative pPLAIII genes in poplar were identified using each genome database. The PgpPLAIIIβ transcripts were observed in all organs of 2-year-old ginseng with the highest in leaves. The transcripts of endogenous pPLAIIIβ from poplar showed the highest expression in vascular tissues such as phloem and xylem, which supports that the major role of pPLAIIIβ is in the development of vascular tissues and cell wall. Overexpression of PgpPLAIIIβ shows reduced plant height with transversely hypertrophied cell growth. The secondary cell wall structure in overexpression lines was altered as well, showing a reduced lignification in xylem. Concomitant therewith, the expression of lignin biosynthesis related genes was decreased. Expression of biosynthesis related genes of auxin, a growth regulating hormone, was also modulated by overexpression of PgpPLAIIIβ. Altogether, cytohistological and its relevant biochemical analysis as well as transcripts changes suggest its role in secondary cell wall development.

GS1-6

144


Enhancement of resveratrol production in peanut sprouts by elicitation

Soyoung Kim1,2, Yu Jeong Jeong1, Suhyun Park1, Jae Cheol Jeong1, Bo-Keun Ha2, Cha Young Kim1*

1Biological Resource Center, Korea Research Institute of Bioscience Biotechnology (KRIBB), Jeongeup 56212, Republic of Korea, 2Department of Plant Biotechnology, College of Agriculture & Life Sciences, Chonnam National

University, Gwangju 61186, Republic of Korea

Resveratrol is a major stilbene compound which is produced in several plants. Resveratrol benefits on human health such as antioxidant, anticancer, anti-heart disease, and anti-diabetes effects. Resveratrol is synthesized by the common phenylpropanoid pathway. Peanuts are a good source of resveratrol in nature and the peanut sprouts have higher resveratrol content than those in peanut seeds. In this study, to enhance resveratrol contents in peanut sprouts, I investigated the production of resveratrol in response to various elicitation. Expression of AhSTS gene was up-regulated by elicitation of 3-day-old sprouts with several elicitors including E2. In addition, E2 treatment significantly enhanced resveratrol content in 10-day-old peanut sprouts. HPLC analysis showed that resveratrol content was about 46-fold higher (44.2 μg/g in fresh weight, FW) in peanut sprouts treated with E2 for 4 days compared with control (0.9 μg/g FW). In addition, expression levels of AhPAL, AhC4H, Ah4CL, and AhSTS genes significantly increased in E2-treated peanut sprouts. Interestingly, epicotyls (460.1 μg/g in dry weight, DW) and hypocotyls (488.1 μg/g DW) had a higher trans-resveratrol content than shoots (2.0 μg/g DW) and roots (58.7 μg/g DW). Taken together, I suggest that elicitor (E2) mediated approach gives offers a valuable system in enhancing resveratrol content in peanut sprouts.

GS1-7


Graduate S

tudent P

resentation


Integrated analyses of shotgun proteomics and metabolomics reveal its association with rise of secondary metabolites of warm water

imbibition soybean seeds

Cheol Woo Min1, Hyejin Hyeon2, Ye Eun Cheon1, Ravi Gupta1, Gi Hyun Lee1, Byong Won Lee3, Hyung Won Ryu4, Jae Kwang Kim2, Sun Tae Kim1*

1Department of Plant Bioscience, Life and industry Convergence Research Institute, Pusan National University, Miryang 50463, Republic of Korea, 2Division of Life Sciences, Incheon National University, Incheon 22012, Republic of Korea,

3Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration, Suwon 16429, Republic of Korea, 4Natural Medicine Research Center, Korea Research Institute of Bioscience and

Biotechnology, Daejun 28116, Republic of Korea

Soybean seeds are known to contain various functional ingredients such as trypsin inhibitor, lecithin, phytic acid, pinitol, saponin, functional peptides, and isoflavones. Recently, the demands of soy-food product supplements have been increased due to their high protein and other secondary metabolites contents. For production of soy-foods or supplements, imbibition of soybean seeds in the water is required step for generation of tofu, soy-milk, and other soy-products. With an aim to get new insight into effects of different imbibition temperature (4, 25, and 55℃), this study conducted integrated proteomics and metabolomics analysis of soybean seeds. For total proteome analysis, we applied TMT labeling based quantitative proteomics combined to FASP (Filter-Aided Sample Preparation) with high-throughput LC-MS/MS. A total of 2,616 proteins were identified out of which 801 proteins showed significantly difference of protein abundance (≥1.5 fold change, Benjamini-Hochberg FDR <0.05) among 4, 25, and 55℃ imbibition seeds. Functional analysis of identified proteins showed an increased abundance of proteins functioning as glycosyl hydrolase enzymes such as beta-glucosidase, alpha and beta-galactosidase, and alpha-mannosidase, or protease, and PTMs related enzymes as well. UPLC TOF-MS analysis showed around 20 fold increase in isoflavone aglycones (daidzein and genistein) while isoflavone glycosides (daidzin and genistin) were decreased in 55℃ imbibition seed, in agreement with proteomics results which we assume positively related to increase abundance of diverse glycosyl hydrolase. A metabolomics analysis revealed 64 metabolites were significantly altered, for example, various free amino acids showed accumulation patterns by increased abundance of various protease enzymes and further confirmed the accumulation of isoflavone aglycones and degradation of raffinose and stachyose in 55℃ imbibition seeds. Based on these results, we recommend the use of 55℃ for soybean seed imbibition to increase the quality of soy-food products.

GS1-8

146


MicroRNA Expression Profiling of Female Developmental Stages of Asian Honeybees (Apis cerana)

Igojo Kang, Chanseok Shin*

Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea

Small non-coding RNAs, such as microRNAs, are key regulators in many biological processes including development. In eusocial insects, especially honey bees, the roles of small RNAome have not been investigated. Given honey bees are one of the major pollinators in agriculture, it is important to investigate the roles of microRNAs in the development of the Asian honeybee (Apis cerana). Here, we performed high-throughput small RNA sequencing of Apis cerana at 14 different developmental stages (i.e., egg, larva 1~6th stages, pupa 1~4th stages of worker bees, nurse bees, forager bees, and drones), and analyzed the differential expression patterns of microRNAs. We identified that Apis cerana has 150 known microRNAs and 31 novel microRNAs. Among them, we further focused on microRNAs expression profiles in female development. Our results suggest that microRNAs are key regulators of growth factors during the development of central nervous system and caste determination in honey bees. Importantly, we provide the first transcriptome analysis of small RNAs in 14 different developmental stages of Apis cerana. Our study suggests that several microRNAs at different developmental stages contribute to regulate the growth of honey bees.

GS1-9


Graduate S

tudent P

resentation


Dissipation Pattern of Insecticide Sulfoxaflor Applied on Minor Crops

Aniruddha Sarker, Sang-Hyeob Lee, Se-Yeon Kwak, Hyo-Jeong Kim, Hye-Rin Jeong, Ae-ji Nam, Jang-Eok Kim*


A greenhouse experiment was carried out to elucidate the residue characteristics of sulfoxaflor and its metabolites (X11719474 and X11721061) in Spinach and Korean Cabbage as model minor crops under different pre-harvest application intervals. The recommended doses of sulfoxaflor sprayed twice with 7 days interval at the 0, 3, 7, 14 days before harvest and the residual amount of pesticide was analyzed using LC-MS/MS. Matrix-matched calibration curves for sulfoxaflor in both spinach and Korean cabbage yielded good linearity (R2≥0.999) with acceptable recovery ranged from 89.0%-107.9%. The biological half-lives of sulfoxaflor in Spinach and Korean Cabbage assessed from first order kinetics of decline (C=3.2539e−0.179t in Spinach and C=1.2432e−0.196t in Korean Cabbage) were 3.87 and 3.54 days, respectively. Pre-harvest residue of sulfoxaflor in Spinach at 3 days prior harvest (2.16 mg/kg) and at harvest day (2.97 mg/kg) below the MFDS-MRL (3.0 mg/kg); in Korean Cabbage, residues at 14 days prior harvest (0.08 mg/kg) below the MFDS-MRL (0.3 mg/kg). Therefore, dissipation pattern and residue at pre-harvest intervals are the key indicator for safety management of pesticide toward quality evaluation of cultivated corps.

GS2-1

148


Development and Validation of Multi-residue Screening Method for 439 Pesticides in Vegetable Juice Using LC-MS/MS and GC-MS/MS

Eunyoung Park1, Yongho Shin1, Junghak Lee1, Jiho Lee1, Mai Rehan1, Jae Chul Cheong2, Hye Suk Lee3, Jeong-Han Kim1*

1Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea, 2Forensic Chemistry Laboratory, Forensic Genetic & Chemistry Division, Supreme Prosecutors’ Office, Seoul 06590, Republic of Korea, 3College of Pharmacy, The Catholic University of Korea, Gyeonggi-do 14662, Republic of Korea

In case of homicide or suicide by pesticide poisoning, it is very important to identify rapidly the unknown toxicant and determine its concentration for detoxification treatment or verification of poisoning. Therefore, the establishment of an accurate, precise, rapid and simultaneous multi-residue pesticide analytical method is critically required. In this study, an effective and simultaneous multi-residue screening method for the determination of 439 pesticides in vegetable juice developed using LC-MS/MS (SHIMADZU LCMS-8060) and GC-MS/MS (SHIMADZU GCMS-8040) for forensic and toxicological investigation. The multiple reaction monitoring was optimized with positive/negative ionization mode on LC-MS/MS and GC-MS/MS. In case of vegetable juice, limit of quantitation (LOQ) was 10 ng/mL for 98.6% of all target analytes. To optimize the sample preparation, all target compounds were spiked in 10 mL of vegetable juice, extracted with acetonitrile (10 mL), treated with magnesium sulfate (4 g) and sodium chloride (1 g) in 50 ml conical tube, and the extracts were centrifuged. To remove interference compounds as a second step, vegetable juice extract was purified using QuEChERS d‐SPE ﴾25 mg PSA and 150 mg MgSO4﴿. And then the extracts (4 μL) were analyzed using LC-MS/MS, and the extracts (2 μL) were analyzed using GC-MS/MS. In the recovery tests at 10 and 50 ng/mL the total average recoveries of all target compounds were from 96.8 to 97.0% (CV≤20%) at two levels. The correlation coefficients (r2) of calibration curves using procedure standard solutions were ≥0.99 for most of target compounds. The averages of matrix effects were 29.8% compounds of all target analytes were included within ±20% of matrix effect in vegetable juice. In conclusion, the established analytical methods in this study can be sufficiently applied at hospitals and forensic facilities in need for the monitoring of multi pesticide residues in vegetable juice samples.

GS2-2


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resentation



Jehyeong Yeon1, Ae Ran Park1, Nan Hee Yu1, Cuong Viet Han1, Ho Myeong Kim2, Hae Woong Park2, Jin-Cheol Kim1*

1Department of Agricultural Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Science, Chonnam National University, Gwangju 61186, Republic of Korea,

2R&D Division, World Institute of Kimchi, Gwangju 61755, Republic of Korea

Organic acids released during the decomposition of amendments have been used in crop fields as benign chemicals to control phytopathogens. However, the use of organic acids against root-knot nematodes (RKN, Meloidogyne spp.) has not been recommended in practice owing to their short retention time in the soil. In this study, we tested the nematicidal activity of organic acids including maleic, fumaric, acetic, acrylic, caprylic, oxalic, and formic acids, as well as copper sulfate against Meloidogyne incognita. Maleic acid showed the strongest nematicidal activity with induction of 100% second-stage juvenile (J2) mortality and 70.47% hatching inhibition at a concentration of 0.37 mmol/L. In addition, the combination of maleic acid and copper sulfate was revealed to have an additive effect in J2 mortality. Application of a wettable powder-type formulation of a mixture of maleic acid and copper sulfate (MIX-WP30) reduced RKN disease on tomato by 51.72% in a pot experiment. It also effectively suppressed gall formation on melon and decreased nematode population density in the soil. Under field conditions, the efficacy of MIX-WP30 (46.73%) was comparable with that of the commercial nematicide fosthiazate (48.38%). This study identified the effectiveness of MIX-WP30 against RKN, and demonstrated that there was an in vivo synergistic effect of MIX-WP30. Based on its nematicidal activity, MIX-WP30 could be used as an effective nematicide for RKN management.

GS2-3

150


Development of Analytical Methods for Chinomethionate in Livestock Products

Seung-Hyun Yang, Hoon Choi*

Department of Bio-Environmental Chemistry, College of Agriculture and Food Sciences, Wonkwang University, Iksan 54538, Republic of Korea

Chinomethionate belongs to quinoxaline fungicide that has been used to control the downy mildew in vegetables. A total of 459 maximum residue level (MRL) has been set for 99 pesticides including chinomethonate in livestock products. This study was conducted to review the pesticide residue method for livestock products in the Food Code and to develop the analytical method for chinomethionate. Test samples were pork, chicken, beef, milk, and eggs. Chinomethionate is a nonpolar compound with a logPow value of 3.8 and its vapor pressure is 0.026 mPa (20℃). Chinomathionate residue on livestock products was detected by GC-ECD. The Method limit of quantitation (MLOQ) was 0.02 mg/kg, considering the maximum residue level (MRL) of chinomathionate as 0.05 mg/kg in livestock product. Chinomathionate was extracted from livestock products using acetone/dichloromethane (9:1, v/v) as solvent with magnesium sulfate and sodium sulfate. Purification step was established with silica gel. Good analysis reproducibilities were obtained (coefficient of variation <5.2%), and the linearity of calibration curves were reasonable (r2>0.995) in the range of 0.01~0.2 mg/kg in various matrices. Recovery tests were carried out at two levels of concentration (MLOQ, 10 MLOQ) and resulted in good recoveries (84.8~103.0%). The analytical method of chinomathionate established in this study confirmed the recovery and reproducibility. This method can be used as a official analysis method for the inspection and safety evaluation of residual pesticides in livestock products.

GS2-4


Graduate S

tudent P

resentation


Spatial distributions of persistent organic pollutants (POPs) and metals in paddy soil from major industrial areas in Korea

Min-Kyu Park1, Jin-Woo Jeon1, In-Gyu Cho1, Sung-Eun Lee2, Sung-Deuk Choi1*

1School of Urban and Environmental Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Republic of Korea,

2School of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea

Contamination of persistent organic pollutants (POPs) and metals is a major concern because of their toxicity and threat to human life and the environment. Particularly, paddy soil contamination is directly linked to human dietary exposure to toxic chemicals via rice consumption. These pollutants, which are emitted from various thermal and industrial processes, can be transported into soils via atmospheric deposition. Therefore, we investigated the levels and spatial distributions of POPs and metals in paddy soils from industrial areas. The soil samples were collected from 50 sites in paddies near industrial complexes in March 2016. For polychlorinated biphenyls (PCBs) and polychlorinated naphthalenes (PCNs), the soil samples were extracted using Soxhlet and accelerated solvent extraction systems, respectively. After clean up, the samples were analyzed using a gas chromatograph/high-resolution mass spectrometer (GC/HRMS). For metals, the homogenized samples were digested with acids (HCl and HNO3). The concentration of Al, As, Ba, Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb, V, and Zn were analyzed using an inductively coupled plasma-optical emission spectrometer (ICP-OES). The soil concentrations of Σ29 PCBs were 216.4–978.6 pg/g dry weight (dw) in Gwangyang, 268.2–1838.3 pg/g dw in Pohang, 273.8–1824.1 pg/g dw in Ulsan. The toxic equivalency quantities (TEQ) from Gwangyang (0.06–0.40 pg-TEQ/g dw), Pohang (0.03–1.03 pg-TEQ/g dw), and Ulsan (0.06–0.22 pg-TEQ/g dw) were lower than the WHO threshold level (20 pg-TEQ/g). The results of principal component analysis (PCA) for PCB homologue patterns demonstrated that each sampling site showed a similar contamination pattern. An Aroclor 1254, which was likely used by steel factories, was identified as a major source of PCBs in Pohang, and were significantly used by various industries including iron and steel industries in Gwangyang and petrochemical and shipbuilding industries in Ulsan. The mean concentrations of Σ44 PCNs were 145.9±101.7 pg/g dw and 95.4±41.4 pg/g dw in Pohang and Ulsan, respectively. The PCN TEQ ranged from 0.006 to 0.065 pg-TEQ/g dw in both sampling areas. The PCN levels in this study are much lower than those at industrial sites in the world. The homologue profiles of PCNs were similar to that of a technical product, such as Halowax, suggesting the usage of Halowax in study areas. Although historical residues from industrial technical products contributed to the contamination of PCNs, combustion-related sources need to be considered in the study areas because of potential combustion sources. The mean concentrations of metals except for Cd (1.98 mg/kg) were below the standards of Korea and Canada. Among the sampling areas, Pohang showed high concentrations of Zn (142.2 mg/kg), and Ulsan and Gwangyang showed high concentrations of Cr (33.9 mg/kg) and Ba (126.4 mg/kg), respectively. These contamination patterns were influenced by the different major industries of each city, which was clearly demonstrated by the PCA results. Pollution indices suggested that metals were enriched in the paddy soils via anthropogenic activities. Further studies are required to understand more clearly the source-receptor relationships of POPs and metals in paddy soil near the industrial areas.

GS2-5

152


Establishment of Soil Management Guideline for Spinach Cultivation in Soils Contaminated with Endosulfan

Se-Yeon Kwak, Sang-Hyeob Lee, Aniruddha Sarker, Hyo-Jeong Kim, Hye-Rin Jeong, Ae-Ji Nam, Jang-Eok Kim*


Many reports have shown that significant amounts of pesticide residues in agricultural soils can be taken up by plant roots, and transferred to edible parts, such as leaves and/or fruits. In this study, endosulfan (ED) was treated on spinach cultivated soil with concentration of 1 and 5 mg/kg and spinach was harvested during 45 days after treatment. The residue amounts of ED in soil and each part of spinach at harvest day were analyzed using GC-ECD. The sum of ED isomer and metabolites which taken up to spinach shoot and root for 45 days from soil were 0.06-1.48 and 0.03-0.39 mg/kg, respectively. Moreover, distribution rates of α-ED in spinach shoot were the highest with 44.1-69.3%, followed by ED-sulfate (17.6-55.9%) and β-ED (0.0-23.1%). From the data of plant uptake experiment, bioconcentration factor (BCF) at time t, the degree to which ED is absorbed from the soil, was calculated as 0.0513-0.1906. Each BCF values were used to obtain mathematical relationship equations (R2 of 0.88-0.99) between time (t) and BCF. Next, permissible concentrations for ED residues in the spinach cultivation soil in order to below MRL (0.05 mg/kg) were calculated as 0.23 mg/kg. However, in general, there can be uncertainty of field experiment due to intra-treatment variation due to soil and pesticide heterogeneity and measurement error related to biomass weights and pesticide analysis and variations in growth conditions, crop cultivars, and soil properties. Finally, the soil management guideline for ED concentration in soils for spinach cultivation can be suggested as 0.2 mg/kg. Therefore, this kind of soil management guidelines for crop cultivation is needed which is similar to maximum residue level (MRL) ensuring the human safety to pesticide residues, but can be considered the uptake of soil residue pesticides.

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Synthesis and evaluation of antibacterial activity of heterocyclic compounds against Staphylococcus species

Jong Hoon Shin, Kwang-sun Kim*


Many researchers predicted that the death rate caused by infections by antibiotic resistant pathogens will be over that from cancer by 2050. Therefore, the development of new therapeutic materials to eradicate such pathogens is in high demand to overcome the infections. Indoles and their derivatives have been regarded as interesting heterocyclic antibacterial molecules in terms that their potential to inhibit the biofilm formation. In this study, we synthesized series of oxindole-derivatives and evaluated their roles as antibacterial actions against Staphylococcus species. From the screening of 20 compounds by Kirby-Bauer method five compounds named as KS15, 16, 17, 19 and 20 showed the antibacterial activity comparable to streptomycin. Moreover, such chemicals were also effective to subspecies and multidrug-resistant of Staphylococcus species although the compounds were inactive to Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa. Additional screening of combinatorial antibiotics identified that mechanistic studies showed that sublethal concentration of KS16 showed the synergistic action on gentamycin and kanamycin which inhibit the protein synthesis, suggesting that KS16 involved in the modulation of cellular level of protein(s). All our results suggest that our newly developed heterocyclic oxindoles are valuable lead antibacterial agents in the eradication of multidrug-resistant Staphylococcus species.

GS3-1

154


LC-MS-Guided Isolation of Anti-inflammatory Compounds from Roots of Pistacia weinmannifolia

Seong Mi Lee1,2, Yang Hee Jo1, Hyung Won Ryu1, Su Ui Lee1, Eun Sol Oh1, Sang Woo Lee3, Sang Ho Choi3, Wan-Yi Li4, Bang Yeon Hwang2, Sei-Ryang Oh1*

1Natural Medicine Research Center, KRIBB, 30-Yeongudanji-ro, Ochang-eup, Cheongwon-gu, Cheongju-si, Chungbuk 28116, Republic of Korea, 2College of Pharmacy Chungbuk National University, 194-21, Osongsaengmyeong 1-ro, Osong-eup, Heungdeok-gu, Cheongju-si, Chungbuk 28160, Republic of Korea, 3International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea,

4Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China

As part of an ongoing search for new anti-inflammatory agents from medicinal plants, five new dihydrochalcones derivatives (1–5) were isolated from the roots of Pistacia weinmannifolia. The structures of pistachalcone A (1), pistachalcone B (2), pistaflavanone A (3), pistachalcone C (4), and pistachalcone D (5) were newly elucidated on the basis of spectroscopic data (1D and 2D NMR, and MS) along with known compounds rhuschalcone II (6), rhuschalcone VI (7) and pauferrol B (8). Our in vitro analysis found that compounds isolated from P. weinmannifolia roots extract exert anti-inflammatory effects in phorbol myristate acetate (PMA)-induced NCI-H292 airway epithelial cells through the suppression of expression levels such as interleukin-8 (IL-8) and mucin 5AC (Muc5AC) that are closely related with pulmonary inflammatory response in the pathogenesis of COPD. Therefore, these pistachalcone derivatives may have values as new starting materials for the development of drug candidates against COPD.

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Comparative toxicity of polyhexamethylene guanidine phosphate from rats (Sprague-Dawley: Wistar: Fischer 344)

In-Hyeon Kim1,2, Sung-Hwan Kim1,2, Doin Jeon1, Hyeon-Young Kim1,3, Kicheon Kim1, Kyuhong Lee1,2*

1National Center for Efficacy Evaluation of Respiratory Disease Product, Korea Institute of Toxicology, Jeollabuk-do 56212, Republic of Korea, 2Department of Human and Environmental Toxicology,

University of Science & Technology, Daejeon 34113, Republic of Korea, 3College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Republic of Korea

The polyhexamethylene guanidine phosphate (PHMG) is the main ingredient in a humidifier disinfectant that caused a severe lung injury such as pulmonary fibrosis (PF). The present study evaluated the toxicological effects of PHMG instillation into the lung of three rat strains to select an appropriate rat model of PF. The PHMG was administrated at the dose levels of 0, 1, and 2 mg/kg in male Spargue-Dawley (SD), Wistar, and Fischer 344 (F344) rats. The animals were sacrificed at 15 days postinstillation. During the test period, clinical signs, mortality, body weight, lung weight, and histopathology were examined. Two rats in the SD PHMG groups and three rats in the F344 PHMG groups were found dead. However, no treatment-related mortality was observed in the Wister PHMG groups. Histopathological analysis showed fibrotic histopathological changes such as interstitial inflammation, granulomatous inflammation/fibrosis, squamous cell metaplasia, bronchioloalveolar epithelial hyperplasia, and alveolar macrophage infiltration and alveolar in the lung of the all PHMG groups. However, eosinophilic cells infiltration was only observed in the SD and Wistar PHMG groups. The apparent discrepancies might be due to strain variation of rats. These results suggest that the most suitable rat model of PF for research would seem to be the PHMG treated Wistar rats because the most marked fibrotic lung histopathological changes occur and there is no treatment-related mortality.

GS3-3

156


Biophotonic Effect of Hypericin in Caenorhabditis elegans

Tram-Anh Ngoc Le, Kyungsu Kang*

Natural Product Informatics Research Center, KIST Gangneung Institute of Natural Products, Gangneung, Gangwon-do 25451, Republic of Korea

Hypericin is a natural product isolated from Saint John’s wort, Hypericum perforatum and widely used as a typical photosensitizer in photodynamic cancer therapy. During the illumination with appropriate wavelength, hypericin is activated and generates subsequent cytotoxic products such as free radicals and singlet oxygen which are known as reactive oxygen species (ROS). In this study, we investigate the effect of hypericin and light treatment on the physiology and longevity of Caenorhabditis elegans, a model nematode. The high concentration of hypericin (500 and 1000 µM) decreased the body length, body bending activity, and the number of egg laid, while 20 µM hypericin had no significant toxicity in C. elegans. We also determined the toxic range of UV (320-400 nm) and orange light (590 nm) in C. elegans based on the observation of the growth and behavior of worms. Through the investigation of a wide range of intensity, exposure time, and treatment interval of light, we finally optimized the hypericin and orange light treatment condition for promoting the general health in C. elegans. Proper amount of hypericin and orange light co-treatment showed a significant increase of the body size and body bending frequency as well as lifespan of C. elegans as compared to those of the control worm. The stress resistant ability of treated worms was also enhanced significantly in the paraquat and heat shock condition. These data suggest that the biophotonic reaction between hypericin and orange light generates a proper amount of ROS, as a result, it enhances the adaptive responses and extends the lifespan of C. elegans.

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Iridoids from the Syringa dilatata flowers and their anti-inflammatory activity

Yeong-Geun Lee1, Jung Eun Gwag1, Hyoung-Geun Kim1, Youn-Hyung Lee2, Dong Sung Lee3, Nam-In Baek1*

1Graduate School of Biotechnology and Department of Oriental Medicine Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea, 2Department of Horticultural Biotechnology of College of Life Sciences, Kyung Hee University,

Yongin 17104, Republic of Korea, 3College of Pharmacy, Chosun University, Gwangju 61452, Republic of Korea

Syringa plants, which have good fragrance and beautiful appearance, are cultivated worldwide. Also this plants have been reported various secondary metabolites such as iridoids, lignans, and phenylethanoids and treatment of tumor, oxidation, and inflammation. In particular, its genus contains a large amount of iridoids that have pharmacological activities. Due to the effectuation of the Nagoya Protocol, securing of biological resources has become important. For this reason we have been interested of S. dilatata, only a Korea native species of its genus. No study has been reported for components and activities of S. dilatata flower. Accordingly research of phytochemical investigation on this plant is valuable. Therefore, the present study focused on the isolation and identification of active materials from this plant, as well as examination for the anti-inflammatory effect of the isolated compounds. Dried flowers of S. dilatata were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned into EtOAc, n-BuOH, and H2O fractions. As a result of repeated SiO2, ODS, and Sephadex LH-20 column chromatographies on n-BuOH fraction, revealed eight new iridoids (6-13) and nine known ones (1-5, 14-17). From the results of spectroscopic data such as NMR, IR, FAB/MS, the chemical structures were identified without ambuiquity. All compounds were isolated for the first time from S. dilatata flowers in this study. Some compounds showed significantly NO inhibition in LPS-stimulated RAW 264.7 macrophages. In addition, quantitative analysis of iridoids in the S. dilatata flowers extract was conducted through HPLC. These results supported that the iridoids isolated from the S. dilatata flowers and its extract are potential as anti-inflammatory agents.

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158


Mushroom derived β-glucan ameliorates high fat diet induced metabolic syndrome in mice

Karthika Muthuramalingam1, Chang Min Choi1, Dharanibalan Kasiviswanathan1, Young Mee Kim1, Moonjae Cho1,2*

1Department of Biochemistry, School of Medicine, Jeju National University, Jeju 63243, Republic of Korea, 2Department of Biochemistry, School of Medicine, Institute of Medical Science, Jeju National University,

Jeju 63241, Republic of Korea

Dietary elements and its resulting metabolites have influential role in shaping the microbiota, which has paramount impact on host associated metabolic disorders. Consumption of high fat diet (HFD) has detrimental effects in deteriorating the host system with dysbiosis in the gut microbial community and obesity. Intervening such metabolic abnormalities with pre- or pro-biotics is emanating as a topic of current interest, since the world has started to highly rely on westernized diet having bountiful supply of fat. Herein this study, we have investigated the effect of mushroom derived β-glucan, a highly soluble dietary fiber, on high fat diet induced obesity and the associated gut microbial imbalances. Apart from inducing anti-obesity nature, β-glucan consumption adds bulk to stool with faster intestinal transit rate (p<0.05) thereby relieving high fat diet induced colonic motility disorder such as constipation. Further, β-glucan intake ameliorates high fat induced stress condition on liver in addition to restoration of intestinal architecture with enhanced expression of tight and adherent junctions. The reduction in goblet cell counts with the consumption of high fat and thus mucin production has been reverted to normal with the inclusion of β-glucan in the diet. More importantly, favorable shift in the gut microfloral community with the β-glucan uptake suppresses the adverse effects of high fat diet consumption. Altogether, the outcomes of the present study indicate that the β-glucan can be used as a promising therapeutic prebiotic in alleviating the high fat diet induced metabolic disorders.

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Synergistic combination of chitosan and aminoglycoside antibiotics against Mycobacterium tuberculosis

Han-woo Kim1, In-dal Park1, Chul-ho Ok2, Won-Kyo Jung3, Ji-yeun Kate Kim1*

1Department of Microbiology, Kosin University College of Medicine, Busan, Republic of Korea, 2Department of Internal Medicine, Kosin University College of Medicine, Busan, Republic of Korea,

3Department of Biomedical Engineering, Pukyong National University, Busan, Republic of Korea

Tuberculosis is a notorious infectious disease caused by Mycobacterium tuberculosis. Treatment for the tuberculosis is based on treatment of primary antibiotics such as rifampin, isoniazid, ethambutol, and pyrazinamide. The resistant tuberculosis to rifampin and isoniazid is treated with secondary antibiotics, and the treatment is performed for 20 months or more and has many side effects. The purpose of this study is to investigate the possibility of developing a novel combination drug using antibiotics and natural products against resistant tuberculosis. Among tested antibiotics and natural products, we have found that combination of aminoglycoside and chitosan showed synergistic antimicrobial effect against mycobacteria. Using four kinds of aminoglycoside antibiotics (Amikacin, Gentamicin, Kanamycin, Streptomycin) and chitosan, we tested their antimicrobial activity against couple of Gram-positive and negative bacteria, Mycobacterium smegmatis, multidrug-resistant M. smegmatis, M. tuberculosis, and mutli-drug resistant M. tuberculosis, mycobacteria, multi-resistant mycobacteria, and common bacteria. The synergistic effect of combinational chitosan and aminoglycoside antibiotics (Amikacin, Gentamicin, Kanamycin) were shown in Mycobacterium strains. Gram-positive Enterococcus faecalis, Staphylococcus aureus and Gram-negative Escherichia coli and Pseudomonas aeruginosa. Also Chitosan was not antimicrobial effect in all bacteria. Among drug-resistant M. tuberculosis, aminoglycoside resistant M. tuberculosis was still resistant to the combinational treatment of chitosan and aminoglycoside. In conclusion, the synergistic antimicrobial activity of combination of chitosan and aminoglycoside is specific to Mycobacteria, and chitosan, which strongly enhances the anti-mycobacterial effect of aminoglycoside, is a good candidate for the combinational drug to multi-drug resistant tuberculosis.

GS3-7

160


Acute Toxicity Assessment of Polyhexamethylene Guanidine Phosphate Following Intravenous Injection and Intratracheal

Instillation in Rats

Hyeon-Young Kim1,2, Sung-Hwan Kim1,3, Doin Jeon1, In-Hyeon Kim1,3, Kicheon Kim1, Kyuhong Lee1,3*

1National Center for Efficacy Evaluation of Respiratory Disease Product, Korea Institute of Toxicology, Jeollabuk-do 56212, Republic of Korea, 2College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Republic of Korea,

3Department of Human and Environmental Toxicology, University of Science & Technology, Daejeon 34113, Republic of Korea

The polyhexamethylene guanidine phosphate (PHMG) was used as an antimicrobial biocide to prevent growth of germs in humidifiers. The present study compared the toxicological differences between intravenous injection and intratracheal instillation of the PHMG in male and female Sprague-Dawley rats. The test article was administered once by intravenous injection to rats at dose of 0, 3.13, 6.25, and 12.5 mg/kg, and by intratracheal instillation to rats at dose of 0, 2, 4, and 8 mg/kg. Male and female rats in the vehicle control groups were administrated with saline through the same route. The rats/sex/group were sacrificed on day 15, after administration of PHMG. During the test period, mortality, clinical signs, body weight, hematology, serum biochemistry, organ weight, gross findings, and histopathology were examined. The change of serum biochemical parameter (BUN, CRTN, and IP) and histopathological change (tubular dilation and atypical tubule) of kidney indicated that the nephrotoxicity injury was induced after intravenous injection with PHMG. The change of lung weight and histopathological change (alveolar macrophage aggregation, de-and regeneration of bronchiolar epithelium, and granulomatous inflammation/fibrosis) of lung indicated that the pulmonary damage was induced after intratracheal instillation with PHMG. The acute intravenous LD50 of PHMG in the male and female rats was 12.2, 12.8 mg/kg, respectively, and target organ was the kidney. On the other hand, the acute intratracheal LD50 of PHMG in the male and female rats was 5.68, 5 mg/kg, respectively, and target organ was the lung. The present study suggest that toxic effect of PHMG can be different in route of administration.

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Immunomodulatory activity of Weissella confusa DD_A7 in zebrafish larvae

Debasish Kumar Dey, Sun Chul Kang*


Dietary effect of probiotics contributing to host health is widely studied. There are many probiotics such as Bacillus, Lactobacillus, Weissella which are traditionally used in food fortification process, but their active contribution is majorly unknown. Therefore, in the present study we investigated the impact of orally administrated Weissella confusa DD_A7 on the immune system of zebrafish larvae. To this aim, we developed a zebrafish model to study the probiotic and pathogenic bacterial interactions. 6 days post-fertilized (dpf) zebrafish larvae were used to screen the foodborne pathogenic bacteria. The larvae were infected via natural oro-intestinal route and identified Escherichia coli 0157:H7 as the most virulent strain inducing a strong inflammatory response and rapid mortality. We pre-colonized zebrafish larvae with DD_A7 and screened for survival upon E. coli 0157:H7 infection. As a result, antagonistic activity of DD_A7 (1×108 colony forming unit (CFU)/mL) was recorded against the pathogens, which correlates the significant reduction of host inflammatory response by inhibiting the NF-κB signaling pathway. Collectively, the finding of the study demonstrated that DD_A7 has prophylactic activity. Its supplementation can improve the immune function by inhibiting the NF-κB and its downstream signaling targets.

GS3-9

162


Development of improved analytical methods for wheat glutenin proteins

You-Ran Jang1,2, Jong-Yeol Lee1*

1National Institute of Agricultural Science, RDA, Jeonju 54874, Republic of Korea, 2Department of Food Science & Technology, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju 54896, Republic of Korea

The wheat gluten proteins are comprised of glutenins and gliadins based on their solubilities in aqueous alcohols. Glutenin proteins mainly contribute to the cohesive and elastic properties of flour dough for the bread-making quality. Based on primary structure, glutenin subunits are divided into high molecular weight (HMW-GS) and low molecular weight (LMW-GS). In this study, we developed analytical methods for wheat gluten proteins to be used in wheat breeding program. HMW-GSs can be identified easily and widely used in breeding programs because it has simple composition. 24 standard cultivars covering all HMW-GSs in hexaploid wheat were analyzed by reversed-phase high-performance liquid chromatography (RP-HPLC). Subunits that could not be distinguished by retention time were separated using SDS-PAGE. RP-HPLC combined with SDS-PAGE can be used to accurate, reliable identification of HMW-GS alleles. However, this method is not suitable to analysis of many breeding lines because of time-consuming. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was optimized and developed for high-throughput analysis of HMW-GSs. HMW-GSs of a wheat cultivars were analyzed every subunits except for only four subunits within one minute by MALDI-TOF-MS and four unidentified subunits were separated using RP-HPLC. MALDI-TOF-MS has the potential for a fast, reliable and accurate analysis of HMW-GSs. In contrast, LMW-GS is difficult to distinguish due to extensive overlapping with the gliadin proteins. To characterize the protein compositions of LMW-GS alleles, 13 near-isogenic lines (NILs) and 10 standard cultivars were used. LMW-GSs were separated by two-dimensional gel electrophoresis (2-DGE) and that separated spots were digested with chymotrypsin and subjected using tandem mass spectrometry (MS/MS) to confirm their identities. We also developed a practical and optimized method for RP-HPLC analysis using a Waters Xbridge BEH C4 peptide column. It is expected to be suitable for the accurate and reliable determination of LMW-GS alleles. Our developed method will be useful to improve wheat end-use quality in wheat breeding programs.

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Jin-Young Choi, Ji-Eun Park, Hyun-Joong Kim, Hae-Yeong Kim*

Department of Food Science and Biotechnology, Graduate School of Biotechnology, Kyung Hee University, Republic of Korea

The value of cotton and the high adoption rate of the stacked cotton have increased the area of GM cotton cultivation in the world. In 2017, GM cotton account for 150,000 tons among 153,100 tons of whole imported cotton into Korea. Some cases have been reported that unintentional leaks of GM cotton into the natural environment caused by the increased GM cotton cultivation and distribution. According to the Biosafety Clearing-House of Korea, currently 13 single-events and 16 stacked-events of GM cotton were approved to import into Korea. Therefore, the effective detection and post-monitoring methods of GM cotton are needed to manage GM cotton efficiently and to prevent its contamination into natural environment in Korea. In this study, a multiplex PCR detection method of GM cotton was developed for faster and more efficient detection. A total of four sets of event-specific tetraplex PCR were performed for 12 single genetically modified cotton events (MON531, MON1445, MON15985, MON88701, MON88913, GHB119, GHB614, COT102, LLcotton25, 281/3006, DAS81910, T304-40xGHB119) except for MON757 event that has ceased commercialization. The detection limit of each set showed a detection value ranging from 0.05% to 1% depending on each set, which is lower than the 3% threshold of the unintentional inclusion allowance of Korea.

GS4-2

164



Thu Thi Nguyen, Chi Rac Hong, Sang Min Kim*

Smart Farm Research Center, KIST Gangneung Institute of Natural Products, Gangneung, Gangwon-do 25451, Republic of Korea

Asparagus (Asparagus officinalis L.) is rich source of phytochemicals, including antioxidants, phenolics, saponins and amino acids. Studies on asparagus revealed a wide range of therapeutic activities, such as anti-diabetic, anti-tumor, antifungal. The aim of this study was to evaluate the quality and functional ingredients of asparagus juice produced by different processing methods. Three processing methods were blending for physical method, hot-water extraction for thermal method, and pectolytic enzyme treatment from Aspergillus niger for enzymatic method, and two fresh asparagus (green and white asparagus) were used to make juice. After processing, the yield of juice solution was measured in a solid state, and antioxidant activity, ingredients such as phenolics, saponin protodioscin and amino acids were evaluated with the freeze-dried juice powder. In result with juice solutions, the enzymatic method showed the highest juice yield among three methods. The juice powder from the physical contained the highest amount of total flavonoids in both asparagus while the juice powder from enzymatic method exhibited higher protodioscin amount in white asparagus. In addition, total phenolics, antioxidant activity and amino acids in both asparagus were changed significantly according to different processing methods. These results will give basic information in the selection of proper extraction method in the production of asparagus juice.

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RDA Database 2.0: Phenolic Acids from Agro-food Resources Based on the Mass Spectrometry

Min-Ki Lee1, Heon-Woong Kim1, Seon-Hye Lee1, Gelila Asamenew1, Suji Lee1, Hyun-Ah Jung2, Sang-Hoon Lee1, Seon Mi Yoo1, Jung-Bong Kim1*

1National Academy of Agricultural Science, Rural Development Administration, 166, Nongsaengmyeong-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do, Republic of Korea, 2Department of Food Science and Human Nutrition, Chonbuk National

University, 567, Baekje-daero, Deokjin-gu, Jeonju-si, Jeollabuk-do, Republic of Korea

The phenolic acid derivatives are naturally occurred secondary metabolites in plants. These numerous compounds are played preventive effects against chronic diseases such as vascular disease, diabetes mellitus, and obesity. From these reasons, basic information of its distribution in Korea agro-food resources has required. The RDA database 2.0 was composed with chemical library and analytical data from 308 species of agro-food resources including different plant species, parts, and, processing. The chemical library of phenolic acid derivatives include a total of 11 categories such as molecular formula and weight, chemical name and structure, positive- and negative- ion fragmentation pattern, UV spectrum pattern, plant resource, literature, etc. The chemical library was constructed from 582 literatures based on structural evidences elucidated by NMR spectroscopy and mass spectrometry, and contained proposed/reported ion fragmentations. A total of 426 individual phenolic acid derivatives was identified and quantified using ultra performance liquid chromatography with diode array detection and quadrupole time of flight/mass spectrometry (UPLC-DAD-QToF/MS). Individual phenolic acid derivatives were provided the contents and their clear analytical data including LC-MS chromatogram and fragmentation pattern in positive ion mode. From the RDA database 2.0, coffee, Fischer's ragwort, mate, perilla leaf, radish sprout, and red-leaf lettuce were showed as the rich sources of phenolic acid derivatives. Furthermore, it is possible to give information for valuable resources of functional food ingredients and drugs to common peoples and scientists.

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PathVisio as a tool for visualizing metabolic network dynamics using metabolic profiling of pepper fruits

Tae Jin Kim1, Hyejin Hyeon1, Tae Gyu Yi2, Nam Il Park2, Jae Kwang Kim1*

1Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Republic of Korea, 2Department of Plant Science, Gangneung-Wonju National University, 7 Jukheon-gil, Gangneung 25457,

Republic of Korea

Nowadays, novel tools have been developed for the efficient analysis and visualization of the large-scale metabolite profile data associated with metabolic pathways. In case of plant metabolome, however, there are many unknown metabolic pathways and many phytochemicals not registered in KEGG pathway. Therefore, PathVisio 3, in which can analyze and draw pathways, was used in order to overcome issue that pathway diagrams cannot be drawn unless metabolites are registered in the KEGG pathway. For the first time, we suggested a high-throughput platform using PathVisio 3 combined with multivariate analysis (principal component analysis, PCA; Pearson’s correlation analysis; hierarchical clustering analysis, HCA). This platform was successfully applied to interpret 10,218 of massive data from 131 metabolites (amino acids, organic acids, sugars, sugar alcohols, free phenolic acids, capsaicinoids, carotenoids, phytosterols, policosanols, tocopherols, volatiles and fatty acids) in 13 peppers with different maturity states and phenotypes. Thus, it revealed that metabolic changes of peppers were depending on maturity and pungency, and it became easy to find out which metabolites have important correlations with others among all the analyzed metabolites. Furthermore, PathVisio 3 was used to present the metabolic changes in pathway diagrams at a glance. It more effectively explained the results of relationships among samples and metabolites associated with common or related metabolic pathway. Thus, PathVisio 3 combined with multivariate analysis could be a high-throughput platform for interpretation of large-scale metabolite profile data.

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Analytical Method Development and Analysis of Azoxystrobin Residue in Sorghum Using LC-MS/MS

Xiu Yuan, Zhehao Jin, Jeonghak Lee, Ahreum Lee, Eunyoung Park, Rakdo Ko, Mai Rehan, Seohyun Kim, Boeun Ju, Jeong-Han Kim*

Department of Agricultural Biotechnology, Seoul National University, Republic of Korea

Azoxystrobin is a fungicide that widely used to protect the crop. In this study, azoxystrobin 21.7% wettable powder was applied on minor crop sorghum, and LC-MS/MS was used to analyze the azoxystrobin and r230310 residue in grain and straw. Pesticide application was carried out 2,000 times dilution. Pesticide application dates were 50/40/30 days, 40/30/21 days, 30/21/14 days, 21/14/7 days before harvest plot. For analysis, Shimadzu LC-MS 8040 (ESI + mode) was used and the method limit of quantitation (MLOQ) was 10 ng/g both in grain and straw. The calibration curve range was 2.5-250 μg/mL, and coefficient r2 was ≤0.99. For the sample preparation, weighed 5.0 g of macerated grain or straw in 50 mL falcon tube, added with 10 mL of distilled water, wetted for 30 minutes. For extraction, added 10 mL of acetonitrile containing 0.1% formic acid and shaking vigorously for 1 minute. After that, added 4 g of MgSO4 and 1 g of NaCl and shaking vigorously for 1 minute, then centrifuged at 3,500 rpm for 5 minutes. For purification, dSPE (7.5 mg GCB, 150 mg PSA, 25 mg MgSO4) was used. After purification, transferred 0.2 mL of the supernatant to an autosampler vial and added 0.2 mL of acetonitrile, analyzed 5 μL with LC-MS/MS. For the method validation, recovery test was carried out at 10MLOQ and 50MLOQ levels. The average recovery rates of azoxystrobin were 97.6% and 99.9% in the grain, 108.9% and 107.2% in the straw, respectively, In the case of r230310, the average recovery rates were 105.5% and 102.2% in the grain, 118.1% and 112.0% in the straw, respectively. As results of the sample analysis, azoxystrobin showed a tendency to decrease over time and r230310 showed a maximum level 0.58 mg/kg in the plot of 30/21/14 days before. Based on these results, it could be used as useful data for the establishment of Maximum Residue Limits (MRLs) and Pre-Harvest Intervals (PHIs).

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Metabolite Profiling and Antioxidant Activity of New Early to Mid-season Apple Cultivars for a Warmer Climate and

Traditional Cultivars

Inhwan Kim, Jihyun Lee*

Department of Food Science and Technology, Chung-Ang University, Anseong 72-1, Republic of Korea

New early to mid-season apple cultivars are being developed to help address warmer growing seasons due to climate change. Free sugars, organic acids, and phenolic composition were determined by HPLC in the pulp and peel of 10 new and 14 traditional apple cultivars. In addition, the phenolic profiles of apple pulp and peel were characterized using high resolution mass spectrometry. Forty-seven polyphenol compounds were identified in apple peel, and nineteen polyphenol compounds were identified in apple pulp by accurate mass. Major compounds in apple peel and pulp were quercetin-3-O-galactoside and chlorogenic acid, respectively. Apple peel had higher phenolic compound levels and antioxidant activity than apple pulp, regardless of cultivars. Two newly developed early-to mid apple cultivars (i.e., Tinkerbell, and Picnic apples) contained higher phenolic compound contents in the pulp than old apple cultivars. Especially, a new apple cultivar, Picnic apples contained 2.75 fold higher levels of the sum of individual polyphenol levels compared to Fuji apples in the pulp. Picnic apples showed similar sugar to acid ratio to Fuji apples. This indicate that the Picnic could have the best quality characteristics in terms of sugar to acid ratios and health promoting activities due to their phenolic profiles.

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Characterization of Phenolic Compounds in the Fruits of Prunus Genus Using UPLC-DAD-QToF/MS

Suji Lee1,2, Heon-Woong Kim1, Min-Ki Lee1, Seon-Hye Lee1, Gelila Asamenew1, Yong-suk Kim2, Jung-Bong Kim1*

1Department of Agro-Food Resources, National Academy of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea, 2Department of Food Science and Technology, Chonbuk National University,

Jeonju 54896, Republic of Korea

Phenolics is widely distributed in plants. Especially, fruits may play a important role in preventing several diseases as phenolics rich source. In this study, phenolic composition and concentration of the Prunus genus were evaluated using ultra performance liquid chromatography with diode array detection and quadrupole time-of-flight mass spectrometric system (UPLC-DAD-QToF/MS). Flavonoids are mainly composed of mono, di-, or tri-glycosides and acylated glycosides with acetic acid based on aglycones of kaempferol and quercetin. A total of 23 flavonoids were isolated and among them, four acetylated flavonols was newly named from Korean cherry and Japanese apricot: quercetin 3-O-(2"-O-acetyl)rutinoside (mumikotin A), quercetin 3-O-(2"-O-acetyl)neohesperidoside (mumikotin B), quercetin 3-O-(4"-O-acetyl)rutinoside (cerakocetin) and kaempferol 3-O-(4"-O-acetyl)rutinoside (cerakorin). Also, a total of 19 hydroxycinnamic acid derivatives (HCAs) were also identified and quantified from Prunus fruits. Japanese apricot and Chinese plum contained the highest amounts of flavonoids while apricot, Japanese apricot and cherry showed the highest amounts of HCAs. Various types of feruloylacetylsucrose and p-coumaroylacetylsucrose were identified in apricot and Japanese apricot, respectively. During the ripening stage of Japanese apricot, as harvest time late, the flavonol and HCAs content was reduced, while the catechin content was increased. It was considered that phenolic acids and flavonols were used for biosynthesis of catechins in maturing. On the basis of phenolic information, PLS-DA model showed different patterns by cluster in various genus and ripening stages.

GS4-8

170


Oral administration of ethanol extract ameliorates atopic dermatitis in house dust mite/DNCB-stimulated NC/Nga mice model

Eui Jeong Han1, Eun Ji Shin2, Min Ju Kim1,2, Disanayaka Mudiyanselage Dinesh Madusanka1,

Mawalle Kankanamge Hasitha Madhawa Dias1, Hyun-Soo Kim3, Seo-Young Kim3, You-Jin Jeon3, Youngheun Jee4, Ginnae Ahn1,5*

1Department of Food Technology and Nutrition, Chonnam National University, Yeosu, Republic of Korea, 2Nantapizza 5000, Damyang, Republic of Korea, 3Department of Marine Life Science, Jeju National University, Jeju,

Republic of Korea, 4Department of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Jeju, Republic of Korea,

5Department of Marine Bio-Food Sciences, Chonnam National University, Yeosu, Republic of Korea

Here, we investigated the therapheutic effect of NC/Nga mice, an atopy mice model. The oral administration of SHE attenuated the atopic dermatitis symptoms, including the skin dermatitis severity, the scratching frequency and TEWL in HDM/DNCB-stimulated NC/Nga mice. The significant attenuation of the serum immunoglobulin (Ig)E, IgG1 and IgG2a levels was observed after the administration of SHE. The histological analysis also revealed that SHE inhibited the epidermal hyperplasia and hyperkeratosis with the dermal infiltrations of mast cells and eosinophil. In addition, SHE reduced the expression levels of cytokines (IL-4, IL-5, IL-6, IL-10, IL-13 and IFN-γ) and chemokines (RANTES, eotaxin and TARC) by decreasing mRNA expression levels of atopy initiators (IL-25, IL-33 and TSLP) as well as the regulation of the mRNA expression levels of T-bet, GATA-3 and STAT-3 in the affected skin. Moreover, the oral administration of SHE led to the reduction of spleen and lymph node size as decreasing the population of immune cells such as eosinophil, inflammatory monocyte, neutrophil and/or macrophage, as well as the mRNA expression levels of IL-4, IL-13, IFN-γ, and TARC. Taken together, these results suggest that the has beneficial potentials for the treatment of allergic diseases. This work (Grants No. M01201920150306) was supported by Korea Institute of Marine Science & Technology Promotion (KIMST).

GS4-9

Poster Presentation



172

PBM-4


Kyungha Lee, Seong Hee Bhoo*

Graduate School of Biotechnology, KyungHee University

Protein purification is an indispensable procedure in biology, biochemistry and molecular biology. Protein purification with high quality and purity is difficult. Several peptide tag systems have been established and are excellent because they have high affinity and specificity for acquiring the target proteins. However, not all peptide based tag systems meet these requirements. For example, the purification of His6-tagged proteins using zinc ion often results in the non-specific co-purification of zinc ion binding proteins. Furthermore we often meet non-specific co-purification of endogenous proteins in certain cell types when using the most popular tag systems such as Flag-tag and Myc-tag. In this case, antibodies with high affinity and specificity are critical requirement for the best protein purification. Most of antibodies have KD values in the low micromolar (10-6) to nanomolar (10-9) range. Even commercial tags such as HA, flag and myc have KD values of 4.5 nM, 6.5 nM and 80 nM respectably. These tags are worldwide used for protein purification but not enough. Here we developed a novel affinity tag based protein purification designated 2B8 tag system. 2B8 tag is consisted of 9 amino acids (RDPLPFFPP) which has an extremely low KD value of picomolar (10-12) range. Since lower KD value means higher affinity, 2B8 tag system will be an outstanding protein purification system even more 2B8 tag is a highly specific to the antibody.


PBM-1

Meloidogyne incognita transcriptome mining during various stages of its development

Chinreddy Subramanyam Reddy, Vimalraj Mani, Soyoung Park, Joon-Soo Sim, Chang-Muk Lee, Bum-Soo Hahn*


Meloidogyne incognita is a root knot nematode belongs to the plant-parasitic nematode group (PPN) are causing a serious loss globally to hundreds of crop plants and it is extremely challenging to control it. This nematode majorly consists five developmental stages namely Egg, J2 (Juvenile), J3, J4 and female stage. It is causing huge loss economically but scary sequence information available. Hence we anticipated to perform transcriptome analysis of its five developmental stages. The cut of value of the transcript FPKM were 0.3 and found 15,798 (egg), 15,564 (J2), 14,908 (J3), 15,226 (J4) and 14,519 (female) transcripts respectively. After trimming and processing of the RNA sequencing data it was mapped to the available M. incognita reference genome and it was covered 51.3 to 62.1%. Further, stage wise mRNA analysis revealed that 17,423 genes were expressed in the transcriptome. Ultimately, by scanning of the RNA sequence we found 12,803 coding transcripts out of 17,423 that were usually express across all the stages that were equal to 73.48%. Conclusively current study gives us basic clues to understand this root knot nematode life cycle and its various developmental stages and also it gives molecular insight to generate lucrative strategies for its regulation.

PBM-2

Profiling and characterization of miRNA target genes in root-knot nematode (Meloidogyne incognita)

Chinreddy Subramanyam Reddy, Vimalraj Mani, Soyoung Park, Joon-Soo Sim, Chang-Muk Lee, Bum-Soo Hahn*


Meloidogyne incognita is a plant parasite that infects through the root in many plants and causes severe damage to the plants. To find the potential targets, we used three target prediction programs and obtained potential target genes (2,431) of M. incognita miRNA. In detail, we obtained 2,560 miRNA genes and 9,018 mRNAs using PITA, whereas 3,036 miRNAs and 15,093 mRNAs using RNAhybrid, further by using miRANDA 2,561 miRNAs and 10,056 mRNAs were identified. Stage-specific miRNA target genes included transcriptional factors, heat shock proteins and DNA repair proteins in the egg stage. Whereas miRNA target genes in J2 encompassed each cell cycle stage proteins, ubiquitin conjugating pathway proteins, heat shock proteins and DNA replication related proteins. The miRNA target genes in J3 stage contained major sperm protein domain containing proteins. We obtained targeting of translation related proteins in J4 stage. In the female stage we found maintaining molybdopterin binding domain containing proteins and ubiquitin mediated protein degradation. Our results may useful to provide fundamental clues to the research of molecular mechanisms to understand the each developmental stage of the root-knot nematode life cycle and assumed to be useful for future plant breeding study of nematode resistance.

PBM-3

Qualitative analysis of cationic metabolites in tomato germplasm using capillary electrophoresis mass spectrometry

Seon-Kyeong Lee, Chinreddy Subramanyam Reddy, Vimalraj Mani, Joon-Soo Sim, Chang-Muk Lee, Soyoung Park, Su-Yeon Kim,

Mahipal Singh Kesawat, Bum-Soo Hahn*


Capillary electrophoresis mass spectrometry is one of the best analytical techniques to separate the analytes even with extremely minute quantity of sample with high efficiency at high speed. In this study, we developed a platform for isolation and relative quantitative analysis of cationic metabolites from the 46 tomato germplasms. Experimental setups (CE-MS interface, background electrolyte and mass spectrometer) were optimized to isolate 20 polar metabolites from these 46 tomato germplasms within 40 minutes. Each identified compound was affirmed using existed source of standard compounds for all the 46 tomato varieties (retention time, accurate mass and enhanced product ions). Principal component analysis showed that the sum of two components accounted for 66% and PCA loading plots showed Solanum lycopersicum varieties (Heinz, Moneymaker, Alisa Craig and Rutgers) were in same group, compared to other varieties. In this study, the major metabolites in tomato germplasm were found to be L-histidine (urea cycle-related metabolism), L-citrulline (urea cycle-related metabolism) and tyramine (BCAA and aromatic amino acids metabolism). In addition, we have proposed a successful relative quantification of polar metabolites to understand the CE-MS method and major metabolites of tomato germplasms. Conclusively our results may provide new insights to unravel the biological functions of uncharacterized process. Futhermore, this approach should be applicable to a wide range of areas for biomarker screening for drug metabolism and pharmacokinetic analysis.

Poster Presentation


Poster Presentation

PBM-7

Myb-like protein, NID1, involved in nitrogen signaling pathway to mediate optimal root development in Arabidopsis

Won Je Lee1, Hojoung Lee2* 1Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology,

Korea University, Anam-dong 5-ga, Seongbuk-gu, Seoul 02841, Republic of Korea, 2Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology,

Korea University, Anam-dong 5-ga, Seongbuk-gu, Seoul 02841, Republic of Korea

Nitrogen (N) is an essential macronutrient for all stages of plant development. But plants cannot fix nitrogen directly; therefore, they must absorb nitrogen from the soil through their roots. Low nitrogen source will not lead to increased production. Therefore, understanding how nitrogen signaling pathways are regulated is essential to the process of maximizing the efficiency of nitrogen use in crops. In this study, we investigated the function of Myb-like gene, NITROGEN RESPONSE DEFICIENCY 1 (NID1), which is an MYBH and MYBD homolog involved in nitrogen signaling. We observed that the root growth performance in the medium supplemented with low concentration of nitrate, atnid1 ko plants showed an enhanced root growth performance than did wild-type plants. Furthermore, AtNID1 promoter:GUS seedlings exhibited strong expressions in root tissues. Taken together, these results indicate that NID1 plays a crucial role as a transcription factor in nitrogen response signaling pathways, leading to the fine optimizing plant growth by controlling the expression of the nitrate response related genes in a given circumstances. Our results suggest that, although MYBC, MYBD and MYBH are homologs, they play different roles during plant growth and development, and these functions should be examined in further studies.

PBM-8

Assessment of synthetic hexaploid wheats in response to heat stress and lead rust infection for the improvement of

wheat production

Hai An Truong1, Won Je Lee1, Masahiro Kishii2, Suk-Whan Hong3, Chon-Sik Kang4, Byung Cheon Lee5, Hojoung Lee1*

1Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong 5-ga, Seongbuk-gu, Seoul 02841, Republic of Korea,

2Global Wheat Program, International Maize and Wheat Improvement Center (CIMMYT), Carretera México-Veracruz Km. 45, El Batán, Texcoco, México, 3Department of Molecular

Biotechnology, College of Agriculture and Life Sciences, Bioenergy Research Center, Chonnam National University, Gwangju 61186, Republic of Korea, 4Crop Breeding Division, National Institute of Crop Science, RDA, Wanju 55365, Republic of Korea,

5Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong 5-ga, Seongbuk-gu, Seoul 02841, Republic of Korea

Bread wheat is a popular cereal crop worldwide, but is now threatened by limited genetic diversity, due to the evolutionary bottleneck to confer abiotic and biotic stresses for future wheat consumption. In contrast, synthetic hexaploid wheat (SHW) is known for its diversity in genetic background, because of the artificial crossing to transfer elite genes from its donors. Therefore, SHW is a potential source for genetic variations to conquer stress. In this study we utilized 2 SHW lines from CYMMIT, namely cSHW339464 and cSHW339465, and a Korean bread wheat, KeumKang, to determine their ability to tolerate heat stress and leaf rust infection. The results showed that cSHW339564 could tolerate heat stress, because of its high accumulation of anthocyanin as well as antioxidant activity, whereas cSHW339465 is resistant to leaf rust, since it can inhibit the growth of pathogen, and activate the activity of β-1,3-glucanase and peroxidase. We hope that these two SHW lines prove to be good candidates to contribute to the improvement and development of the current resource of wheat.

PBM-5

Phylogenetic relationships of the Cultivated Mulberry (Rosales: Moraceae) in Korean

Wan Taek Ju*, O Chul Kwon, Hyun Bok Kim, Ji Hae Lee


This study was carried out to understand phylogenetic relationships of the 30 mulberry cultivars converved in Korea based on the ITS rDNA region, and they were compared to 40 reference sequences from GenBank. The size and the G+C content of the ITS rDNA gene regions from the 30 Korean mulberry cultivars and 40 reference sequences varied from 612-630 bp and 58.19-61.62%, respectively. Based on the results of the comparative phylogenetic analysis of the ITS rDNA regions of the 30 Korean mulberry cultivars and 40 reference sequences, they were divided into three groups (Group 1, 2, and 3) and two subgroups (Group 1A and 1B within Group 1). The sequence lengths of the Korean mulberry cultivar numbers 1-26 and 27-30 were 615 bp and 616 bp, respectively. At 205 bp location of ITS1 rDNA region, the cultivar numbers 1-26 contain the nucleotide thymine but the cultivar numbers 27-30 contain the nucleotide adenine. In addition, the insertion of the nucleotide adenine at 206 bp location was found only in the four Korean mulberry cultivars (numbers 27-30). Based on these sequence information and phylogenetic result, the 30 Korean mulberry cultivars were identified as M. alba and M. australis. This study will contribute to the construction of genetic database constructions and accurate variety identifications for unidentified mulberry varieties in Korea.

PBM-6

Development, Identification and Validation of Specific Markers for Antler-shaped Ganoderma lucidum



In this study we identified single nucleotide polymorphism (SNP) and sequence characteristic amplification region (SCAR) markers for specific identification of antler-shaped Ganoderma lucidum strains. When the partial mitochondrial SSU rDNA gene sequence of various antler- and kidney-shaped G. lucidum strains were analyzed and aligned, an SNP was found only in the antler-shaped G. lucidum strain at position 456 bp. In addition, this SNP of antler-shaped strains was digested by HinfI restriction enzyme. We further analyzed the polymorphism of various G. lucidum strains by random amplified polymorphic DNA (RAPD) analysis. In RAPD analysis, we isolated and sequenced a fragment, specific for antler-shaped G. lucidum strains. Based on this specific fragment sequence, two sets of specific primer pairs for antler-shaped G. lucidum strains were designed. PCR analysis revealed that two specific bands were observed only from antler-shaped strains. These two molecular markers will be helpful for identification of morphological characteristics of G. lucidum.


174

PBM-12

Regulation of photosynthetic activity by an Isoform of the plastid RNA polymerase-associated protein FSD3

Geupil Jang*, Sangyool Lee

School of Biological Sciences and Technology, Chonnam National University

Plastid-encoded RNA polymerase (PEP) plays a crucial role in chloroplast biogenesis by regulating the expression of photosynthesis genes. Approximately 12 PEP-associated proteins (PAPs) including FSD3 regulate PEP activity and chloroplast development by modulating establishment of the PEP complex. In this study, we identified FSD3S, a splicing variant of FSD3; the FSD3 and FSD3S transcripts encode proteins with identical N-termini, but different C-termini. Characterization of FSD3 and FSD3S protein showed that the C-terminal region of FSD3S contains a transmembrane domain, which promotes FSD3S localization to chloroplast membrane but not to nucleoids unlike FSD3. We also found that overexpression of FSD3S negatively affects photosynthetic activity and chloroplast development by reducing expression of genes involved in photosynthesis. In addition, FSD3S failed to complement the defects of chloroplast development in the fsd3 mutant. These results suggest FSD3 and FSD3S with the distinct localization pattern have different functions in chloroplast development, and FSD3S negatively regulates expression of PEP-dependent chloroplast genes, and development of chloroplasts.

PBM-9

Characterization of two leaf rust-resistant Aegilops tauschii accessions for developing synthetic wheat

Cao Son Trịnh1, Aro Lee1, Won Je Lee1, Minseo Kim1, Hyeri Lee1, Duleepa Pathiraja1, In-Geol Choi1, Namhyun Chung1, Chon Sik Kang2,

Byung Cheon Lee1, Hojoung Lee1* 1Department of Biosystems and Biotechnology, College of Life Sciences and

Biotechnology, Korea University, Anam-dong 5-ga, Seongbuk-gu, Seoul 02841, Republic of Korea, 2Crop Breeding Division, National Institute of Crop Science, RDA,

Wanju 55365, Korea, Republic of Korea

Aegilops tauschii (Ae. tauschii) is a diploid (2n=2x=14) wild grass species, which has been reported as the progenitor of hexaploid wheat (Triticum aestivum) with D-genome. In this study, 68 Ae. tauschii accessions with diverse geographical backgrounds were investigated for their resistance to infection by the leaf rust fungi Puccinia triticina. Two Ae. tauschii accessions that exhibited hyper-resistance to leaf rust at both seedling and adult stages were identified. Utilizing two susceptible Ae. tauschii ecotypes and keumkang, a common Korean wheat cultivar known to be susceptible to leaf rust, as the negative control, further investigations were conducted for understanding the mechanism underlying immunity to leaf rust disease of these two resistant accessions. Ae. tauschii is a remarkable genetic source, especially for abiotic and biotic stress resistance genes, as the plant is known for its wide-ranging geographical habitat and adaptability to different environments. This, combined with the fact that Ae. tauschii and wheat share a close evolutionary relationship, is indicative of the immense benefit of using Ae. tauschii as a material for improving the quality of synthetic wheat. Our aim was to identify and evaluate the strongest Ae. tauschii contenders for breeding leaf rust-resistant synthetic wheat.

PBM-10

Bacillus subtilis strain Y16 promotes nitrate reductase activity and elicits enhanced growth performance in Arabidopsis

Seokjin Lee1, Chan Young Jeong1, Won Je Lee1, Cao Sơn Trịnh1, Hai An Truong1, Suk-Whan Hong2, Hojoung Lee1*

1Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong 5-ga, Seongbuk-gu, Seoul 02841, Republic of Korea, 2Department of Molecular Biotechnology, College of Agriculture and Life Sciences,

Bioenergy Research Center, Chonnam National University, Gwangju

PGPR (plant-growth-promoting Rhizobacteria) is a bacterium that helps plants growth in the rhizosphere. PGPRs is known to involve in various mechanisms, thereby reinforcing the development of plant. We screened out the PGPR which are effective in early plant growth by handling PGPR on media where Arabidopsis thaliana grows. We purified a PGPR Bacillus subtilis strain Y16, using this method. When we placed Bacillus subtilis strain Y16 near the roots, seedlings showed notably stronger growth than control, particularly in biomass and root hair. Quantitative reverse transcription polymerase chain reaction analysis revealed the high level of expression of the high affinity nitrate transporter gene, NRT2.1. After considering how BsY16 could promote plant growth, we focused on nitrate, which is essential to plant growth. The nitrate content was lower in A. thaliana treated with BsY16. Examination of the activity of nitrate reductase revealed higher activity in plants treated with PGPR than in the control. These results suggest that BsY16 promotes assimilation and use of nitrate in plants, which promotes plant growth.

PBM-11

Molecular signaling axis in bleomycin-induced pulmonary pathogenesis in A549 cells

Karthika Muthuramalingam1, Changmin Choi1, Dharanibalan Kasiviswanathan1, Youngmee Kim1*, Moonjae Cho2*

1Department of Biochemistry, School of Medicine, Jeju National University, Jeju 63243, 2Department of Biochemistry, School of Medicine, Institute of Medical Science,

Jeju National University, Jeju 63241

Pulmonary fibrosis results in a build-up of scar tissue in the lungs, which is a progressive respiratory disease with worldwide prevalence. Apart from genetic and non-genetic factors, fibrosis occurs as a side effect of bleomycin antineoplastic treatment. Amidst arising concerns with bleomycin induced fibrosis, the molecular signaling pathway behind the development of this detrimental condition remains unclear till date. Insights into the cellular and molecular mechanisms enable us to develop effective antifibrotic treatment strategies. Herein this study, we investigated the molecular axis behind bleomycin induced lung fibrosis using human alveolar epithelial cells (A549 cells). From the experimental observation, is a progressive respiratory disease with worldwide prevalence imposing increasing burden in-terms of hospitalization and death. Apart from genetic and non-genetic factors, fibrosis occurs as a side effect of bleomycin antineoplastic treatment. Amidst arising concerns with bleomycin induced fibrosis, the molecular signalling pathway behind the development of this detrimental condition remains unclear till date. Insights into the cellular and molecular mechanism enables us to develop effective antifibrotic treatment strategies. Herein this study, we investigated the molecular axis behind bleomycin induced lung fibrosis using human alveolar epithelial cells (A549 cells). From the experimental observation, it was shown that TGF-β acts as a central mediator in the progression of fibrosis as a result of epithelial-mesenchymal transition (EMT) and senescence crosstalk upon bleomycin treatment thereby advancing this devastating fibrotic condition.

Poster Presentation


Poster Presentation

PBM-15

Intramitochondrial transfer and engineering of mammalian mitochondrial genomes in yeast

Young Geol Yoon1*, Michael Koob2 1Department of Biomedical Science, Jungwon University, 2Institutes for Translational

Neuroscience & of Human Genetics, Department of Laboratory Medicine and Pathology, University of Minnesota

Mitochondrial genomes (mtDNA) depend on the nuclear genome with which they have evolved to provide essential replication functions and have been known to replicate as xenotransplants only in the cells of closely related species. We now report that complete mouse mitochondrial genomes can be stably transplanted into the mitochondrial network in yeast devoid of their own mtDNA. Our analyses of these xenomitochondrial yeast cells show that they are accurately replicating intact mouse mtDNA genomes without rearrangement and that these mtDNA genomes have the same overall topology as the mtDNA present in the mouse mitochondrial network (i.e., circular monomers). Moreover, non-mtDNA replication and selection sequences required for maintaining the mitochondrial genomes in bacterial hosts are dispensable in these yeast mitochondria and could be efficiently and seamlessly removed by targeted homologous recombination within the mitochondria. These findings demonstrate that the yeast mtDNA replication system is capable of accurately replicating intact mammalian mtDNA genomes without sequence loss or rearrangement and that yeast mitochondria are a highly versatile host system for engineering complete mammalian mitochondrial genomes.

PBM-16

Characterization and Antifungal Activity of Chitinase Producing Bacteria

Philip Ahn, Woo-Jin Jung*

Department of Agricultural Chemistry, Institute of Environmentally-Friendly Agriculture (IEFA), College of Agriculture and Life Sciences Chonnam National University

In this study, chitinase-producing bacteria having chitinolytic activity on colloidal chitin-containing agar medium were isolated from soil. The isolated three bacteria were identified that Bacillus australimaris, B. stratosphericus and Jiella aquimaris by 16S rRNA sequencing. The chitinase activity and cell growth of three bacteria was in the colloidal chitin medium measured by days of incubation. Crude enzyme from three bacteria showed an active bands of chitinase isozymes on SDS-PAGE gel. The antifungal activities of chitinase-producing three bacteria were examined on the growth of Fusarium oxysporum KACC 40032 in vitro. Also each of bacteria inhibited the mycelial growth of phytopathogenic fungi. The decomposition patterns of chitin in chitinase concentration at 0.1U/ml was analyzed by TLC. These results suggest that B. australimaris, B. stratosphericus and Jiella aquimaris could be possibility of biocontrol agents for fungal disease on plants. These material advance of the biorational agriculture in field application would be feasible. [This work was supported by a grant from the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry through the Agri-Bioindustry Technology Development Program, funded by the Ministry of Agriculture, Food and Rural Affairs (no.316032-5).]

PBM-13

Production of anti-MERS-CoV S protein monoclonal antibody for inhibition of MERS-CoV infection by epitope peptide-CpG-

DNA-liposome complex without carriers

Byoung Kwon Park1, Sony Maharjan1, Su In Lee1, Jinsoo Kim1, Joon-Yong Bae2, Man-Seong Park2, Younghee Lee3, Hyung-Joo Kwon4*

1Center for Medical Science Research, Hallym University College of Medicine, 2Department of Microbiology, Korea University, 3Department of Biochemistry, Chungbuk

National University, 4Department of Microbiology, Hallym University College of Medicine

Middle East Respiratory Syndrome Coronavirus (MERS-CoV) utilizes spike (S) glycoprotein for recognition of receptor and enters into target cells and plays the vital part in infection. In this study, we selected two epitope peptide sequences within receptor binding domain (RBD) of the S protein of MERS-CoV. We used complex comprising the epitope peptide of MERS-CoV S protein and CpG-DNA encapsulated with a phosphatidyl-β-oleoyl-γ-palmitoyl ethanolamine (DOPE): cholesterol hemisuccinate (CHEMS) to immunize mice, and produced monoclonal antibodies 506-2G10G5 and 492-1G10E4E2. Western blotting data showed both monoclonal antibodies detected S protein and immunoprecipitated the native form of S protein. Indirect immunofluorescence and confocal analysis suggested strong reactivity of monoclonal antibodies 506-2G10G5 and 492-1G10E4E2 towards S protein of MERS-CoV virus infected Vero cells. However, 506-2G10G5 monoclonal antibody treatment significantly reduced the plaque percentage in MERS-CoV infected Vero cells in comparison to normal mouse IgG and 492-1G10E4E2 treatment. Thus, we successfully produced monoclonal antibody directed to RBD domain of S protein which could be used in the development of diagnostics and therapeutic purposes in future.

PBM-14

Production of bacteria-reactive IgM antibodies by CpG-DNA to enhance phagocytic activity against

Staphylococcus aureus infection

Te Ha Kim1, Dong Bum Kim2, Hee Su Lee1, Min Hyung Kwak1, Avishekh Gautam1, Madhav Akauliya1, Joongwon Park1, Jinsoo Kim1, Hanseul Lee1, Sang Kyu Park3,

Younghee Lee3, Hyung-Joo Kwon1* 1Department of Microbiology, Hallym University College of Medicine,

2Center for Medical Science Research, Hallym University College of Medicine, 3Department of Biochemistry, Chungbuk National University

CpG-DNA modulates proliferation and differentiation of immune cells resulting in the increased immune responses. The presence of natural IgM antibodies in normal serum was reported, however, relevance of CpG-DNA with the production of bacteria-reactive IgM antibodies was never investigated. Here, we proved the function of CpG-DNA concerning the production of bacteria-reactive IgM antibodies. The CpG-DNA administration leads to the increase production of bacteria-reactive IgM antibodies both in peritoneal fluid and serum via TLR9 signaling pathway. When we stimulated B cells with CpG-DNA, production of bacteria-reactive IgM antibodies was reproduced in vitro. Furthermore, the bacteria-reactive monoclonal IgM antibodies, which are purified from CpG-DNA-stimulated peritoneal cavity of the mice, enhanced the phagocytic activity of RAW 264.7 cells against S. aureus MW2 infection. Therefore, we suggest that CpG-DNA enhances the antibacterial activity of the immune system by triggering the production of bacteria-reactive IgM antibodies. Considering the enhanced phagocytic activity of bacteria-reactive IgM antibodies against bacterial infection, we suggest possible application of the antibodies for the treatment of antibiotic-resistant bacterial infections.


176

PBM-20

Diplacone isolated from paulownia tomentosa fruits induces MPT-mediated necrotic cell death in A549 cells

Myung-Ji Kang1,2, Hyung Won Ryu1, Eun-Bin Kwon1,2, Su Ui Lee1, Sei-Ryang Oh1, Dong-Oh Moon3, Mun-Ock Kim1*

1Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 2College of pharmacy, Chungbuk National University,

3Department of Biology Education, Daegu University

We evaluated the anticancer effect of the geranylated flavonoid diplacone (DP) isolated from Paulownia tomentosa fruit on A549 human lung carcinoma cells. DP inhibited cell proliferation through necrotic cell death with extensive cytoplasmic vacuoles derived from mitochondria. We have found that DP induces an obvious increase in mitochondria Ca2+ levels, generation of ROS and loss of mitochondria membrane potential (MMP). Moreover, treatment of ruthenium red (an inhibitor of mitochondrial Ca2+ uniporter), BAPTA-AM (Ca2+ chelator) or NAC (ROS scavenger) effectively inhibited the DP-mediated formation of vacuoles, the loss of MMP and necrotic cell death. Finally, we have been shown that inhibition of mitochondrial permeability transition (MPT) by cyclosporin A apparently inhibits the DP-mediated loss of MMP and cell death, suggesting that cell death by DP on A549 cells is mediated by MPT. Further studies are still needed to determine how the mitochondrial Ca2+ activates MPT and to identify actual cell death executors after MPT activation. Overall, this study demonstrated that activation of MPT due to excessive accumulation of mitochondria Ca2+ and ROS is a critical mediator in DP-mediated necrotic cell death. These finding suggests that DP may be valuable for the development of new adjuvant drug to treat lung cancer. In this study, we first demonstrated the anti-proliferative effect of DP and its mechanism in A549 cells.

PBM-17

The Current Species Compotent of Bemisia tabaci in China and South Korea

Chenliang Guo1, Bueyong Park2, Gwan- Seok Lee2, Dong Chu1, Yong- Zhe Zhu1, In- Hong Hong2*

1Key Lab of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, China,

2Department of Crop Life safety Division of Crop Protection, National Institute of Agricultural Sciences, Korea

The genetic structure analysis of alien species may be associated with the introduction routes and potential mechanism of invasions. There were at least three introduced species of Bemisia tabaci (MEAM1, MED, and JpL species) determined in South Korea. However, the invasion pathway and patterns of the alien B. tabaci in greenhouse in South Korea remains poorly unknown. In the present study, we analyzed the composition and distribution of mitochondrial haplotypes of B. tabaci MED collected from 7 provincial level administrative units in Korea in 2018 based on the mtCOI. Our results revealed three haplotypes (abbreviated as Hap1, Hap2, and Hap3) were present in B. tabaci MED in Korea based on mtCOI fragment analysis. Among the haplotypes, Hap2 was dominant (98.98%), followed by Hap3 (0.68%) and Hap1 (0.34%). Our result suggests that there were at least three introduction of B. tabaci MED into Korea, which may come from the eastern Mediterranean and western Mediterranean regions. This is the first report on the B. tabaci MED of eastern Mediterranean introduced into South Korea, indicating a new introduction into South Korea. The potential mechanisms contributing to the dominance of the Hap2 of B. tabaci MED in South Korea are also discussed. The genetic structure analysis will be helpful to reveal the cryptic invasion of alien whitefly in South Korea.

PBM-18

Identification and Characterization of Saikosaponins as Antagonists of Transient Receptor Potential A1 channel

Yongmun Choi*, Gyeong Been Lee, Jungeun Yang, Jongsuk Lee, Changon Seo

Biomaterials R&D Team, Gyeonggido Business & Science Accelerator Bio Center

Neuropathic complex syndrome which is caused by a lesion or disease of the somatosensory system. Currently available therapeutics for the treatment of neuropathic pain include tricyclic antidepressants, serotonin noradrenaline reuptake inhibitors, the anticonvulsants gabapentin and pregabalin, and opioids. However, in addition to adverse effects, these drugs showed limited response in patients with neuropathic pain, providing a rationale to explore new drug classes acting on novel targets and with better efficacy/safety profile. Transient receptor potential (TRP) channel family members that are expressed in nociceptors attracted a great deal of attention in the research of novel therapeutics for neuropathic pain. TRPA1 has been reported to senses a variety of chemical irritants including endogenous products of tissue injury and inflammation, which activate sensory neurons to elicit acute or persistent pain. Recent years have seen a growing interest in the discovery of TRPA1 antagonists as potential therapeutics for neuropathic pain. In this study, in an effort to discover new pharmacological modalities of TRPA1 inhibition, we performed a screening campaign of natural compounds that are derived from medicinal plants and microorganisms. The efforts led to the identification of saikosaponins that inhibited agonist-stimulated Ca2+ influx in vitro and that alleviated AITC-induced nociceptive responses in vivo. These observations serve as a basis for further elaboration of saikosaponins with the goal of providing new insight into the treatment of neuropathic pain.

PBM-19

Molecular genetic characterization of orevactaene biosynthesis in Epicoccum nigrum and its application

on the production of its derivatives

Yoonji Lim, Eun-ha Choi, Hyung-Jin Kwon*

Department of Biological Sciences and Bioinformatics, Myongji University

Orevactaene (OVT) is a yellow polyketide product of Epicoccum nigrum (syn. E. purpurascens) and composed of a polyunsaturated acyl chain and a 4-hydroxy-2-pyrone with a C-linked galactosyl moiety. When applied to the acetate pathway, the acyl chain is predicted to convey four C-methylations on α-carbons and one of these methyl moieties is oxidized to carboxylic acid. OVT was isolated with its HIV inhibitory activity. The OVT isomer, epipyrone A was known to possess a telomerase inhibitory activity and other biological activities. We localized the putative OVT biosynthetic gene cluster (BGC) from E. nigrum genome sequence by searching the highly-reducing fungal polyketide synthase (HR-fPKS) gene that was clustered with a glycosyltransferase gene. The OVT BGC is composed of HR-fPKS (OvtA), a NDP-sugar-utilizing glycosyltransferase (OvtB), a cytochrome P450 (OvtC) and a transporter (OvtD). Nearby these Ovt genes localized are the genes encoding a FAD-dependent monooxygenase (Ovt-ORF2), a transcription factor (Ovt-ORF3) and an oxygenase (Ovt-ORF4). In order to confirm the role of this BGC in OVT biosynthesis, the OvtA gene was inactivated in E. epicoccum KACC 40642, by gene replacement with a neomycin phosphotransferase resistance cassette (nptII). The resulting ΔOvtA::nptII recombinant (ΔOvtA) was incapable of producing OVT. Further study will elucidate the biosynthetic mechanism of OVT, providing the molecular genetics strategy for overproduction and combinatorial biosynthesis.

Poster Presentation


Poster Presentation

PBM-23

Stimulating DDX3 expression by Serotonin 5-HT receptor 7 through phosphorylation of p53 via the AC-PKA-ERK

signaling pathway

Khoa Nguyen, Junho Lee*


DDX3 is a host viral factor that can inhibit the hepatitis B virus induced innate immune responses. In this study, the 20 bioactive compounds were screened the effects on DDX3 and we found that 5-HT upregulated DDX3 promoter activity via the 5-HT7 receptor on liver hepatocellular cells (HepG2 cells) by using a luciferase assay, RT-PCR analysis, and western blot analysis. Furthermore, we are trying to elucidate the pathways involved in the stimulating effect of 5-HT on DDX3 expression to induce innate immune responses against hepatitis B virus infection. A knockdown of the 5-HT7 receptor by transfection si-5-HT7 receptors or si-control into HepG2 cells treated by 5-HT (or 5-HT plus agonist) confirmed the role of the 5-HT7 receptor in DDX3 expression. The IFN-β-Luc expression and level of hepatitis B virus surface Antigen (HBsAg) showed that DDX3 mediated by the 5-HT7 agonist (AS-19) increased IFN-β expression and inhibited HBV replication. Luciferase assays showed the involvement of 5-HT7 receptors in DDX3 expression via cAMP/AC/PKA pathways by using protein kinas A (PKA) and adenylyl cyclase inhibitor (MDL 12330A). Overall, 5-HT7 was found to be a new potential target to inhibit hepatitis B infection by activating AC/PKA/ERK pathways by phosphorylating p53 via the 5-HT7 agonist response by mediating DDX3 expression.

PBM-24

Molecular determinants of α3β4 nicotinic acetylcholine receptors inhibition by triterpenoids



In a previous work, we reported the regulatory role of the triterpenoids on 5-HT3A receptors activity in Xenopus laevis oocytes 1). In the present report, we studied the modulation of triterpenoids on the activity of the human nicotinic acetylcholine receptor type α3β4. Two-electrode voltage clamp experiments were used to test acetylcholine mediated inward current (IACh). Treatment with triterpenoids (dehydroeburicoic acid, 6α-hydroxypolyporenic acid C and pachymic acid) inhibited IACh in a concentration dependent and reversible manner. The inhibitory regulation of IACh by each triterpenoid showed in a non-competitive manner on the activity of α3β4 nicotinic acetylcholine receptors. These results show that triterpenoids (pachymic acid, dehydroeburicoic acid, 6α-hydroxypolyporenic acid C) can be used as agents to modulate the activity of nicotinic acetylcholine receptor type α3β4. Furthermore, molecular docking studies of 6α-hydroxypolyporenic acid C on α3β4 nicotinic acetylcholine receptors in silico showed that this molecule interacted predominantly with residues at cavities in the α3 subunit and β4 subunit. This docking assays indicated four potential binding sites for this ligand in the extracellular region at sensor domain of α3β4 nicotinic acetylcholine receptors. All taken together, this study revealed that molecular basis of α3β4 nicotinic acetylcholine receptors by triterpenoids and provides a novel potent interaction ligand.

PBM-21

RCVpW-based insecticide resistance monitoring and mechanism of spinosad resistance in Thrips palmi

Ju Hyeon Kim1, Min Ju Kim2*, Kyungmun Kim2*, Tian-Bo Ding3*, In Hong Jeong4, Deok Ho Kwon1*, Si Hyeock Lee2

1Research Institute of Agriculture and Life Sciences, Seoul National University, 2Department of Agricultural Biotechnology, Seoul National University, 3College of Plant Health and

Medicine, Qingdao Agricultural University, 4Division of Crop Protection, National Institutes of Agricultural Science, Republic Development Administration

A residual contact vial plus water (RCVpW) bioassay method was established to monitor insectiside resistance in field populations of the melon thrips, Thrips palmi. Resistance level against six major insecticides were evalutated in five regions to test applicability of RCVpW as an on-site resistance monitoring tool. Reduced mortality in response to six test insecticides were exhibited compared to the RDA susceptable strain showing 100 % mortality, indicating different degree of resistance. An apparently reduced mortality to emamectin benzoate and chlofenapyr was observed in some field populations, suggesting uneven distribution of resistance to these insecticides in field populations. In addition, spinosad resistance was high and widely distributed in the test regions. Synergistic bioassay revealed that cytochrome P450-mediated metabolic factor is involved in spinosad resistance in the Korean population.

PBM-22

Characterization of venom components in 14 Aculeate bees/wasps via venom gland transcriptome analysis

Kyungjae Andrew Yoon1, Do Eun Lee2*, Woo Young Bang3*, Chang Hwan Bae3*, Joo-Hong Yeo3*, Young Ho Koh4*, Si Hyeock Lee1*

1Research Institute for Agriculture and Life Sciences, Seoul National University, 2Department of Agricultural Biology, Seoul National University, 3National Institute of Biological Resources (NIBR), Environmental Research Complex, 4Ilsong Institute of

Life Science, Hallym University

To identify and compare the venom components and expression patterns of some bees/wasps, venom gland-specific transcriptome analyses were conducted for 14 Aculeate bees/wasps. Most of the allergens and pain-producing factors showed extremely high expression levels in social wasps, implying that social wasps have evolved to use venom to defend the colony against intruders. Acid phosphatase and tachykinin, which are known as allergens and neurotoxic peptides, were found with high frequencies in the venom glands of solitary wasps. This suggests that solitary wasps might use their venom for catching and preserving prey. In the venom glands of bumblebees, little or no transcripts of major allergens or pain producing factors were identified, implying that bumblebees venoms are relatively less toxic than those of social or solitary wasps. Taken together, the differential expression patterns of venom genes in some Aculeate bees/wasps implies that bees/wasps have unique groups of highly expressed venom components, which appear to have evolved in response to both ecological and behavioral influences.


178

PBM-28

Identification of plant host factors associated with cucumber mosaic virus infection by proteomic and

transcriptomic approaches

Soo-Jung Han, Jang-Kyun Seo*

Department of International Agricultural Technology, Seoul National University

While the most efficient method to control viral diseases is currently to grow virus resistant crops, a detailed understanding of the molecular interactions between viruses and host plants is crucial for developing virus resistant crops. In this study, we aimed to identify and characterize host factors interacting with cucumber mosaic virus (CMV) that causes severe damages in various crops, including pepper. We utilized proteomic and transcriptomic approaches to identify the host factors. In the proteomic approach, three CMV proteins, 1a, 2a, and MP, were fused with the Flag tag and expressed in plant cells using recombinant CMV infectious cDNA constructs. Among the FLAG-tagged constructs, the recombinant CMV clone carrying a FLAG-tag at the N-terminus of 2a were competent for replication. To identify 2a-interacting host proteins, FLAG-tagged 2a was immunoprecipitated and the resulting product was subjected to SDS-PAGE and LC-MS/MS analyses. In the transcriptomic approach, we analyzed pepper transcriptomic regulation associated with CMV infection. Comparative analysis of differentially expressed genes showed that various resistance-related genes were transcriptionally regulated by CMV infection. Our approaches can provide new insights into enhancing plant immunity against viral diseases.

PBM-25

Hyaluronic acid production with galactose and glucose in Escherichia coli W3110

Seong Woo Kwon, Ji Eun Woo, Yu-Sin Jang*

Institute of Agriculture & Life Science (IALS), Department of Agricultural Chemistry and Food Science Technology, Division of Applied Life Chemistry,

Gyeongsang National University

Hyaluronic acid is a polymer structure of glucuronic acid and N-acetyl glucosamine, which can contain moisture multiple times than its molecular weight, and commonly used in cosmetics. Hyaluronic acid is produced by extracting the animal tissues (from crab, shrimp shell, rooster combs, and human umbilical cords). Extraction from animal tissues has few critical problems such as the risk of cross-species viral infection, hyaluronic acid complex with proteoglycans and problem of ethics. For instead of extraction from animal tissues, the researchers developed biosynthetic pathways for the production of hyaluronic acid from Streptococcus sp. But streptococcus sp. need expensive media, few applicants for DNA modifications and pathogenic. To overcome the above problems we selected Escherichia coli W3110 as the host for the production of hyaluronic acid. [This work was supported by a grant from the Ministry of Science and ICT (MIST) through the National Research Foundation (NRF) of Korea (NRF-2016R1D1A3B04933184).]

PBM-26

HR-MAS NMR, UPLC-QTOF/MS and GCxGC-TOF/MS-based metabolomics study for age discrimination of ginseng

Dahye Yoon1, Bo-Ram Choi1, Seohee Ma2, Jae Won Lee1, Ick-Hyun Jo1, Young-Seob Lee1, Geum-Soog Kim1, Suhkmann Kim2, Dae Young Lee1*

1Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, 2Department of Chemistry, Center for Proteome Biophysics and

Chemistry Institute for Functional Materials, Pusan National University

Ginseng is a perennial crop, and 4-to-6-year-old ginseng is usually used for the medicines. It is considered that ginseng’s medicinal properties are dependent on the cultivation period. Therefore, the price of ginseng is dependent on the cultivation period. The purpose of this study was to discriminate the age of ginseng based on metabolomics. Both the primary and secondary metabolites of a ginseng were analyzed by using high-resolution magic angle spinning nuclear magnetic resonance (HR-MAS NMR) spectroscopy, ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS), and gas chromatography quadrupole time-of-fight mass spectrometry (GC-TOF/MS). Powdered ginseng samples were analyzed except the extraction process using HR-MAS NMR spectroscopy. UPLC-QTOF/MS was used for the analysis of secondary metabolites. GCxGC-TOF/MS was used for the comprehensive and sensitive analyzing of volatile and semi-volatile organic compounds in the ginseng.The primary metabolites detected from HR-MAS NMR, the levels of fumarate and choline showed moderate prediction with AUC value of more than 0.7. As a result of UPLC-QTOF/MS-based profiling, 61 metabolites mainly contributed to discriminate age of ginseng. The results of GCxGC-TOF/MS showed clear discrimination of 4-, 5-, and 6-year-old ginsengs using OPLS-DA to 100% of discrimination rate. Metabolomic approach showed the potential to discriminate the age of ginseng. In this result, profiling of metabolites and multivariate statistical analyses can discriminate the age of ginseng.

PBM-27

Differential association of BsSIG5 with multiple regions in plastid genome depending on light condition

in Bienertia sinuspersici

Yen Le Thi, Lorrenne Caburatan, Eunsun Park, Hwan Namgoong, Joonho Park*

Department of Fine Chemistry, Seoul National University of Science and Technology

The plastid transcriptional regulation in higher plants requires two different types of RNA polymerase, nuclear-encoded RNA polymerase (NEP) and plastid-encoded RNA polymerase (PEP). Especially Sigma factors are able to initiate gene transcription associated with PEP. Bienertia sinuspersici has one of the well-known single cell C4 species. In one cell, it contains dimorphic chloroplasts, central chloroplast (CC) and peripheral chloroplast (PC). Each chloroplast remains its own genome and expression system. This study carried out for understanding the function of BsSIG5, sigma factor 5 in B. sinuspersici. Expression level of BsSIG5 gene was increased during leaf development. In mature chlorenchyma cell, BsSIG5 proteins were accumulated in both CC and PC, not like BsSIG1 which was specifically localized in CC. To understand the transcriptional regulation in dimorphic chloroplasts, chromatin immunoprecipitation was performed. These results showed differential associations in promoter regions of several chloroplast genes depending on light condition. These results suggested that BsSIG5 could be involved in differential transcription regulation in B. sinuspersici during leaf maturation.

Poster Presentation


Poster Presentation

PBM-31

Synthesis of methylated anthranilate derivatives using engineered strains of Escherichia coli

Hye Lim Lee, Songyi Kim, Eun Ji Kim, Da Ye Han, Bong-Gyu Kim, Joong-Hoon Ahn*


Anthranilate derivates are used as flavoring and fragrant agents for a long period time. Recently, these compounds gain an attention due to the new biological functions including antinociceptive and analgesic activities. Three anthranilate derivatives, N-methylanthranilate, methyl anthrannilate, and methyl N-methylanthranilate were synthesized using metabolically engineered stains of Escherichia coli. NMT encoding N-methyltransferase from Ruta graveolens, AMAT encoding anthraniloyl-coenzyme A (CoA): methanol acyltransferase from Vitis labrusca, and PqsA encoding anthranilate Coenzyme A ligase from Pseudomonas aeruginosa were cloned and E. coli strains harboring these genes were used to synthesize the three desired compounds. E. coli mutants (metJ, trpD, tyrR mutants), which provide more anthranilate and/or S-adenosyl methionine, were used to increase the production of the synthesized compounds. MS/MS analysis was used to determine the structure of the products. Approximately, 185.3 μM N-methylanthranilate and 95.2 μM methyl N-methyl anthranilate were synthesized. This is the first report about the synthesis of anthranilate derivatives in E. coli.

PBM-32

Synthesis of Acridone using engineered Escherichia coli

Gyu Sik Choi, Hye Jeong Choo, Joong Hoon Ahn*


Acridone alkaloid is one of the heterocyclic compounds that contains wide range of pharmaceutical and chemotherapeutic activities such as anticancer, antiviral, anti-inflammatory, antimalarial and antimicrobial activities. Acridone alkaloids are synthesized by some plant species such as Citrus maxima, Ruta graveolens and Toddaliopsis bremekampii. Acridone is synthesized from anthranilate. We carried out the synthesis acridone in Escherichia coli. Acridone synthase (ACS) along with anthranyl-CoA ligase was transformed into E. coli and the synthesis of acridone was examined. In order to increase the endogenous anthranlate, we tested several constructs having various genes in shikimate pathway and selected the best construct. To supply more malonyl-CoA, a second substrate for acridone, acetyl coenzyme A carboxylase (ACC) genes from Photorhabdus luminescens were overexpressed. After selecting the best combination of genes, approximately 129.64 mg/L of 1,3-dihydroxy-9(10H)-acridone was synthesized.

PBM-29

Biosynthesis of N-2-hydroxybenzoyl tryptamine using engineered Escherichia coli

Songyi Kim, Joong-Hoon Ahn*


Phenolic compounds are secondary metabolites of plants, more than 10,000 phenolic compounds have been known and they contained various biological activities. 2-Hydroxybenzoic acid, having the C6-C1 skeleton makes a conjugation with amines. N-2-hydroxybenzoyl tryptamine, formed by conjugation of salicylic acid and tryptamine, was reported to contain anti-convulsant, anti-nociceptive activities. In this study, we synthesized N-2-hydroxybenoyl tryptamine from 2-hydroxybenzoic acid and tryptamine in engineered Escherichia coli harboring HCT (hydroxycinnamoyl transferase), hBAD (hydroxy benzoate coenzyme A ligase). Furthermore, TDC (tryptamine decarboxylase), entC (isochorismate synthase) and pchB (isochorismate pyruvate lyase) were introduced into E. coli along with HCT and hBAD in order to synthesize N-2-hydroxybenzoyl tryptamine from glucose. To further increase the yield of N-2-hydroxybenzoyl tryptamine, we used a E. coli mutant and overexpressed genes in the shikimic acid pathway. Resultantly, about 227.2 mg/L N-2-hydroxybenzoyl tryptamine was synthesized.

PBM-30

Microbial synthesis of chiral p-coumaroyl glycerol

Min Kyung Song, Geun Young Sim, Su Jin Lee, Bong-Gyu Kim, Mihyang Kim, Taegum Lee, Youhoon Chong, Joong-Hoon Ahn*


Synthesis of chiral compounds is not an easy task; therefore, biological systems could be applied to chiral synthesis. Glycerol is a prochiral molecule that could be useful for the synthesis of diverse biological active compounds when it becomes chiral. We mimicked suberin biosynthesis in plants to synthesize a chiral p-coumaroyl glycerol using engineered Escherichia coli. By supplying glycerol as a substrate for p-coumaroyl glycerol and a carbon source for E. coli, approximately 799.9 mg/L of p-coumaroyl glycerol was synthesized. For unequivocal determination of the absolute configuration of the synthesized p-coumaroyl glycerol, enantiomerically pure (R)- and (S)-isomers were prepared by chemical synthesis. Specific rotation of the synthesized p-coumaroyl glycerol matched the authentic (S)-isomer, with an optical purity of 98.6%.


180

PBM-36

Dual functions of Arabidopsis thaliana ascorbate peroxidase 1 are related to structural conformation

Shubhpreet Kaur1,2, Prapti Prakash1,2, Moon-Soo Chung1, Sungbeom Lee1,2, Jin-Hong Kim1, Seung Sik Lee1,2*

1Research Division for Biotechnology, Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI), 2Department of Radiation Science

and Technology, University of Science and Technology (UST)

Plants respond to various external stimuli by generating excessive amount of reactive oxygen species (ROS). Plant defense system consists of both enzymatic and non-enzymatic antioxidant systems to counteract ROS. Ascorbate peroxidase is a class I heme-containing peroxidase enzyme which functions as a scavenger of H2O2 using ascorbate as electron donor. In this study, we have demonstrated that Arabidopsis thaliana cytosolic Ascorbate peroxidase 1 (AtAPX1) exists in diverse molecular forms and plays dual role as peroxidase and molecular chaperone. SDS-PAGE, native PAGE and SEC analysis proved the existence of APX1 in monomeric and oligomeric forms. The key factor behind the dual role of AtAPX1 proteins is molecular switching from low molecular weight (LMW) form to high molecular weight (HMW) complexes or vice versa. The heme inhibition test by potassium cyanide effectively inhibited peroxidase activity but did not affected the chaperone activity of AtAPX1 which indicates little or no involvement of heme moiety in chaperone function. Our results indicate the underlying correlation between the various conformations of AtAPX1 protein and role switching between peroxidase and chaperone.

PBM-33

Synthesis of chlorogenic acid from glucose in Escherichia coli

Hye-Lime Lee, Joong-Hoon Ahn*


Chlorogenic acid are dietary source of phenolic compounds found abundantly in coffee, fruits, and vegetables. Chlorogenic acid has been used for the protection against side effects of chemotherapy and prevention of cardiovascular disease and cancer. Chologenic acid is a conjugated with cafferic acid and quinic acid. Its biosynthesis in Escheria coli has been reported. However, synthesis of cholorogenic acid from glucose in one E. coli was not been successful because aroD needed to be delete for the synthesis of quinic acid, which did not allow the synthesis of caffeic acid. In order to circumvent this problem, we overexpressed various combination of genes in shikimic acid pathway, which expected to increase the accumulation of both substrates without blocking any pathway. In addition, we coexpressed hydroxycinnamate-CoA quinate transferase (HQT) and 4-coumarateCoA:ligase (4CL) along with construct containing various combination of genes in shikimate pathway. Using this strategy, we produced 303 mg/L of chlorogenic acid, which was much higher yield than the previous report using two culture system.

PBM-34

Biosynthesis of 2,4-Dihydroxyquinoline in engineered Escherichia coli

Hye Jeong Choo, Joong Hoon Ahn*


Quinoline derivatives are used for the synthesis of antimalaria compounds. They are also known as anti-inflammatory and antimicrobial agents. We attempted to synthesize 2,4-dihydroxyquinoline (DHQ), which can serve a precursor for the synthesis of various derivatives, using Escherichia coli. DHQ can be synthesized by two steps from anthranilate, an intermediate in shikimate pathway of E. coli. First, anthranilate needs to be converted into anthraniloyl-CoA by CoA ligase (CoL). Then, anthraniloyl-CoA is conjugated with malonyl-CoA by polyketide synthase (PKS). We selected CoL and PKS to synthesize DHQ from anthranilate. Using the selected CoL and PKS, we synthesized DHQ from glucose. In order to increase the final yield of DHQ by providing more substrates anthranilate and malonyl-CoA, we introduced constructs containing combination of shikimate pathway genes and acetyl-CoA carboxylase complex. Furthermore, we used a mutant E. coli strain which accumulated more anthranilate. Using these approaches, 753.7 mg/L DHQ was synthesized.

PBM-35

Bioinformatic approaches to identify novel gene targets responsible for adriamycin resistance in A549 cells

Dharanibalan Kasiviswanathan1, Jeong Yong Moon2, Somi Kim Cho2,3,4*, Moonjae Cho1,5*

1Department of Biochemsitry, School of Medicine, Jeju National University, Jeju 63243, Korea, 2Subtropical/Tropical Organism Gene Bank, Jeju National University, Jeju 63243, Korea, 3Interdisciplinary Graduate Program in Advanced Convergence Technology and

Science, Jeju National University, Jeju 63243, Korea, 4Faculty of Biotechnology, College of Applied Life Sciences, SARI, Jeju National University, Jeju 63243, Korea, 5Institues of

Medical Sciences, Jeju National University, Jeju 63243, Korea

Non-Small Cell Lung Cancer (NSCLC), is one of the major types which accounts for 85 % of lung cancers worldwide. Developing acquired resistance against chemo drugs is one of the major drawbacks in cancer treatment. Also, NSCLC can be diagnosed in their advanced stages (Stage III or IV) that leads to a poor survival rate. The purpose of this work is to improve our intellect to identify the potential gene targets in Adriamycin resistance in A549 cells. In this work, we established acquired ADR resistance from A549 NSCLC cell line (ADR-A549). The consequence of acquired ADR resistance responsible gene targets and their regulation in signalling pathways were analysed using RNA sequencing. We found 400 DEGs with either up or down regulation. And so, we retrieved the DEGs associated with biological functions (GO), biological pathways functional protein interaction networks (PPI) and miRNA targets, DNA repair mechanism and Pyruvate metabolism gene targets in ADR-A549 cell lines. We also predicted a list of potential miRNAs from DEGs of ADR-A549 cells. Further, we used Kaplan Meir plotter analysis to identify whether any of our gene targets are relevant survival biomarkers. Altogether, our work highlights the list of potential gene targets in ADR-A549 responsible for drug resistance that could be potential therapeutic targets.

Poster Presentation


Poster Presentation

PBM-39

Induction of apoptosis by mono(2-ethylhexyl) phthalate via enhanced generations of nitric oxide and reactive oxygen

species in wild-type and p53-null human lymphoblastoid cells

Eun Mi Park, Min Young Kim*


Mono-(2-ethylhexyl) phthalate (MEHP), the active metabolite of the plasticizer of synthetic polymers di-(2-ethylhexyl) phthalate (DEHP), which has been demonstrated to be a reproductive toxicant in rodents, but the mechanism by which MEHP exposure result in human cells is still unclear. To elucidate toxicity mechanism of MEHP, we investigated its apoptotic effects and regulation of nitric oxide (NO•) and reactive oxygen species (ROS) production in human lymphoblastoid TK6 (p53-WT) and NH32 (p53-null) cells. MEHP inhibited cell growth and promoted apoptosis by the elevation of NO• and ROS in a dose- and time-dependent manner. Apoptosis was delayed and greatly reduced in magnitude in NH32 cells, underscoring the importance of p53 modulation of the response. These results suggested that MEHP could induce apoptosis of human lymphoblastoid cells through p53-induced upregulation of NO• and ROS.

PBM-40

Development of a film-based immunochromatographic microfluidic device for malaria diagnosis

Yong Tae Kim, Jihye Choi, Heungsop Shin*


In this study, a novel film-based immunochromatographic microfluidic device has been developed for malaria diagnosis. A microfluidic channel was patterned on a polyethylene terephthalate double-sided adhesive film using a plotting cutter and was assembled with a polycarbonate (PC) film. The PC film used for the probe immobilization layer was activated using oxygen plasma treatment to modify the film surface with avidin-biotin linker to immobilize a capture antibody. A fluorescent labeled Pan type mAb conjugate was prepared for signal indicator after undergoing a sandwich enzyme-linked immunosorbent assay. Target antigens include Plasmodium falciparum LDH and Plasmodium vivax LDH which were injected into the sample inlet. Target antigens combined with the conjugate and then flowed to the detection chamber where two test dots and a control dot exist. In the presence of P. falciparum LDH, three detection dots including test dot 1, test dot 2 and Ctrl revealed fluorescence signals where P. falciparum mAb, Pan type pLDH mAb and goat anti-mouse IgG were immobilized, respectively. When P. vivax LDH was present, T2 and Ctrl dots showed fluorescence signals while no signal was detected with the negative control. P. falciparum LDH and P. vivax LDH were successfully detected on the IMD with a detection limit of 50 ng/mL and 100 ng/mL, respectively.

PBM-37

Synthesis of 4-hydroxybenzoic acid derivates in Escherichia coli

Han Kim, Geun Yong Sim, Songyi Kim, Joong-Hoon Ahn*


4-Hydroxybenzoic acid are substrate for various chemicals including 3,4-hydroxybenzoic acid (protocatechuic acid; PCA) and methylparaben. We used Escherichia coli to synthesize PCA and methylparaben. PCA is known as an antioxidant and has anti-inflammatory and anti-cancer activities. Paraben is an ester between 4-hydroxybenzoic acid (4-HBA) and alcohol (methanol or ethanol). It is wildly used preservatives in cosmetic and pharmaceutical products. We introduced a gene to synthesize 4-hydroxybenzoic acid from chorismite. For the synthesis of PCA, two additional genes (ubiC and pobA) were introduced. For the synthesis of methylparaben, three genes (hbad, ubiC and EHT1) were coexpressed. To increase the chorismate, several constructs containing various combinations of genes in shikimate pathway were tested and the best construct was selected. Using these approaches, 902.95 mg/L PCA and 432.75 mg/L methylparaben were synthesized.

PBM-38

Genome-wide Identification and Functional Characterization of Drought-Responsive Long Noncoding RNAs (DRIL) in Rice

Nuri Oh1, Jimin Lee2*, Daeheon Jang2*, Choonkyun Jung1,2*1Institutes of Green Bio Science and Technology, Seoul National University,

2Graduate School of International Agricultural Technology, Seoul National University

Long noncoding RNAs (lncRNAs) have appeared as critical regulatory factors of various biological processes in both plants and animals. In Rice, it has been known that several lncRNAs regulate key biological processes such as phosphate homeostasis, flowering and fertility. However, systematic examination of rice lncRNAs involved in abiotic stress responses has not been reported. Here, we re-analyzed the expression profile of lncRNAs in publicly available rice transcriptome datasets derived from abiotic stress treatments to unveil the potential roles of rice lncRNAs in abiotic stress responses. Overall, we identified 10,831 rice lncRNAs that were significantly altered in shoot and/or root tissues under four different stresses. Based on Venn diagram analysis, we observed strong cross-talks between different stress signaling pathways, showing transcriptional regulatory networks underlying lncRNA expression changes in response to abiotic stresses. In addition, we identified novel drought-induced lncRNAs (DRIL) through transcriptome analysis of drought-treated rice. Real-time RT-PCR analysis confirmed the differential expression patterns of these lncRNAs under various stress conditions. To determine the regulatory role of lncRNAs in abiotic stress signaling, LncRNAs were transiently overexpressed in rice protoplasts. As a result, LNC_Os08g32065 (DRIL1) overexpression increased the expression levels of stress marker genes such as Wsi18, glutamate dehydrogenase, and GEM-like protein. Our results show the first comprehensive identification and functional characterization of a group of abiotic stress-responsive lncRNAs in rice.


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PBM-44

Investigation of physio-molecular response to water-deficit stress in Korean F1 maize (Zea mays L.) hybrids

Won Min Jeong, Dong Kyu Jeong, Dong Yeol Lee, Young Min Goo, Young Sook Kil, Seung Mi Sin, Hyeong Hwan Lee, Sang Gon Kim*


Despite the relevance of drought stress, the regulation of gene expression, protein accumulation, and plant physiology under water-deficit stress is not well understood in Korean F1 maize (Zea mays L.) hybrids. In this study, we investigated the effect of water deficit on the F1 maize hybrids, Ilmichal (Ilmi) and Gwangpyeongok (GPOK), by withholding water for 10 days during flowering. Water deficit severely reduced the relative water content (RWC), area, SPAD values, and conductance of leaves, stem length, and the dry matter content of aerial tissues in drought-stressed plants of both hybrids. However, the dry matter content of roots was reduced only in GPOK. Two-dimensional gel electrophoresis identified 24 spots representing proteins accumulated to differential levels in well-watered and drought-stressed plants of both hybrids. Among these proteins, delta 3,5-delta 2,4-dienoyl-CoA isomerase (spot 8) and bifunctional 3-phosphoadenosine 5-phosphosulfate synthetase 2 (spot 23) were present only in GPOK, whereas NAD-dependent epimerase/dehydratase (spot 13), NAD(P)H-quinone oxidoreductase subunit 2 A (spot 24), and an uncharacterized protein (spot 19) were present only in Ilmi, in response to water-deficit stress. Semi-quantitative reverse transcription PCR analysis showed that the transcript levels of most of the genes encoding these proteins correlated well with their protein levels, suggesting that water deficit affects gene transcription in F1 maize hybrids at the flowering stage.

PBM-41

Mutations on a turn and loop other than in ligands of copper in amicyanin affect the volume of active site

Youngkook Moon1, Moonsung Choi2*, Sooim Shin1,3*1Interdisciplinary Program of Bioenergy and Biomaterials Graduate School, College of

Engineering, Chonnam National University, Gwangju 61186, Republic of Korea, 2Department of Optometry, College of Energy and Biotechnology, Seoul National University of Science and Technology, Seoul 01811, Republic of Korea, 3Department of Biotechnology

and Bioengineering, College of Engineering, Chonnam National University, Gwangju 61186, Republic of Korea

Amicyanin is a type 1 copper protein mediating electron transfer between methylamine dehydrogenase (MADH) and cytochrome c-551i for energy production. We present in here what kind of residues in amicyanin affect to the size of room for active site belonging copper of amicyanin. The method that we applied is the measurement and calculation of angles and distance among 4 ligands for copper; His 53, Cys 92, His 95 and Met 98 in all published mutant amicyanins mathematically. The results demonstrated 3 things that volume of active site is larger in reduced form of amicyanin than in oxidized form, His 95 play a major role in deciding room size, and the residues on a turn and loop not ligands of copper influence distance change between His 95 and copper resulting in volume change of active site.

PBM-42

Stereolithographic Printing of Microfluidic Vascular Chip using Biocompatible Hydrogels

Eun-Jeong Choi, Heungsop Shin*, Yong Tae Kim*


Development of a three-dimensional vascular microfluidic chip is important to study vascular-related diseases and new drugs. However, realization of in vivo-like blood vessel chip based on softlithographic fabrication method has limitations such as complex fabricating process, tedious manual labor, and prone to assembly failure. In this study, we developed a 3D vascular microfluidic chip by utilizing a stereolithographic printer. The microfluidic chip was fabricated using a resin made of 30% (v/v in water) poly(ethylene glycol) diacrylate (MW =700) a UV initiator (Irgacure-819) and a photosensitizer (2-isopropylthioxanthone) for constructing a biocompatible hydrogel structure. The 3D-printed blood vessel is transparent and air permeable that enables us to culture a cell and observe the cell differentiation. Endothelial cells were cultured to mimic the in vivo-like blood vessel in the 3D-printed chip. After EAhy926 cells cultivation, cell viability was confirmed by a live/dead cell assay and cultured cells were stained with Anti-Rabbit CD31 labeled with Alexa Fluor 488 and DAPI for demonstrating a vascularization, and visualizing nuclei, respectively. Creating the blood vessel with 30% PEG-DA-700 hydrogel using automated 3D-printing process reduces the fabrication steps and time, avoids an assembly and bonding, and economizes manufacturing cost. We believe our stereolithographic 3D-printing method for fabricating a blood vessel provides a cost-effective, convenient, simple, and reproducible routes to vascular-related disease monitoring and drug screening analysis.

PBM-43

Morphological and molecular characterization of mutants related to distorted trichomes in tomato

Jae-In Chun1, Won-Ki Hong1, Seong-Min Kim1, Jin-Ho Kang1,2*1Graduate School of International Agricultural Technology and Crop Biotechnology Institute, Seoul National University, 2Institute of GreenBio Science and Technology,

Seoul National University

Trichomes are the first epidermal cells that develop from the aerial epidermis on leaves, stems and other organs on many plant species. Trichomes have the function of physical and chemical defense biotic and abiotic stresses. Although they are very important in plant defense, most of the genes involved in trichome development are unknown in tomato. To identify genes related to trichome development, we screened Micro-Tom mutant population generated by ethylmethane sulfonate (EMS) mutagenesis and selected four mutants which have distinct trichome phenotypes compared to wild-type plants. All four mutants have a similar trichome morphology with twisted and distorted trichomes. Previously, we showed that hairless and inquieta mutants have distorted trichomes and the corresponding genes are involved in the polymerization of actin cytoskeleton. So we hypothesized that the new four mutants are also related to actin polymerization. To compare the expression levels of ARP2/3-WAVE complex genes involved in the polymerization of actin cytoskeleton between the four mutants and wild-type plants, RT-PCR analysis was performed. Among 16 genes tested, one gene showed different size between one mutant and wild-type plants. To find mutation region from the mutant, we are doing cDNA and genomic DNA sequencing of this gene from the mutant and wild-type plants.

Poster Presentation


Poster Presentation

PBM-47

Overexpressing wheat low-molecular-weight glutenin subunits in rice (Oryza sativa L. japonica cv. Koami) seeds

Su-Bin Lee1, Kyoungwon Cho2, Sun-Hyung Lim1, Jong-Yeol Lee1*1National Institute of Agricultural Science, RDA, Jeonju 54874, Korea, 2Department of Biotechnology, Collge of Agriculture and Life Sciences, Chonnam National University,

Gwangju 61186, Korea

Genes encoding wheat low-molecular-weight glutenin subunits (LMW-GSs) that confer dough strength and extensibility were previously identified from Korean wheat cultivars. To improve low viscoelasticity of rice (Oryza sativa L.) dough caused by the lack of seed storage proteins comparable to wheat gluten, two genes, LMW03 and LMW28, encoding LMW-GSs are cloned from Korean wheat cultivar Jokyoung. The LMW genes are inserted into binary vectors under the control of the rice endosperm-specific Glu-B1 promoter. Transgenic rice plants expressing LMW03 or LMW28 in their seeds are generated using Agrobacterium-mediated transformation. The expression of recombinant wheat LMW-GS in the transgenic rice seeds was confirmed by SDS-PAGE and immunoblot analysis. Their accumulation in the endosperm and aleurone layers of rice seeds was observed through in situ immuno-hybridization.

PBM-48

Cellular Localization of Wheat High Molecular Weight Glutenin Subunits in Transgenic Rice Grain

Jae-Ryeong Sim1, Kyoungwon Cho2, Sun-Hyung Lim1, Jong-Yeol Lee1*1National Institute of Agricultural Science, RDA, Jeonju 54874, Korea, 2Department of Biotechnology, College of Agriculture and Life Sciences, Chonnam National University,

Gwangju 61186, Korea

Rice (Oryza sativa L.) is a primary global food cereal. However, when compared to wheat, rice has poor food processing qualities. Dough that is made from rice flour has low viscoelasticity because rice seed lacks storage proteins that are comparable to gluten protein from wheat. Thus, current research efforts aim to improve rice flour processing qualities through the transgenic expression of viscoelastic proteins in rice seeds. In this study, we characterized the transgenic expression of wheat glutenin subunits in rice seeds. The two genes 1Dx5_KK and 1Dy10_JK, which both encode wheat high-molecular-weight glutenin subunits that confer high dough elasticity, were cloned from Korean wheat cultivars KeumKang and JoKyung, respectively. These genes were inserted into binary vectors under the control of the rice endosperm-specific Glu-B1 promoter and were expressed in the high-amylose Korean rice cultivar Koami (Oryza sativa L.). Individual expression of both glutenin subunits was confirmed by SDS-PAGE and immunoblot analyses performed using T3 generation of transgenic rice seeds. The subcellular localization of 1Dx5_KK and 1Dy10_JK in the rice seed endosperm was confirmed by immunofluorescence analysis, indicating that the wheat glutenin subunits accumulate in protein body-II and novel protein body types in the rice seed. These results contribute to our understanding of engineered seed storage proteins in rice.

PBM-45

Towards reducing the immunogenic potential of wheat flour: omega gliadins encoded by the D genome of hexaploid wheat may also harbor epitopes for the serious food allergy WDEIA

Jong-Yeol Lee1*, Sun-Hyung Lim1, Susan B Altenbach2

1National Institute of Agricultural Science, RDA, Jeonju 54874, Korea, 2USDA-ARS, Western Regional Research Center, 800, Buchanan Street, Albany, CA 94710, USA

Omega-5 gliadins are a group of highly repetitive gluten proteins in wheat flour encoded on the 1B chromosome of hexaploid wheat. These proteins are the major allergens in a severe form of food allergy called wheat-dependent exercise-induced anaphylaxis. A mutant line missing low-molecular weight glutenin subunits encoded at the Glu-B3 locus was selected previously from a doubled haploid population generated from two Korean wheat cultivars. Analysis of flour from the mutant line by 2-dimensional gel electrophoresis coupled with tandem mass spectrometry revealed that the omega-5 gliadins and several gamma gliadins encoded by the closely linked Gli-B1 locus were also missing as a result of a deletion of at least 5.8 Mb of chromosome 1B. However, two minor proteins showed strong reactivity to patient sera in both the parental and the mutant lines and also reacted with a monoclonal antibody against omega-5 gliadin. Analysis of the two minor reactive proteins by mass spectrometry revealed that both proteins correspond to omega-5 gliadin genes encoded on chromosome 1D that were thought previously to be pseudogenes. While breeding approaches can be used to reduce the levels of the highly immunogenic omega-5 gliadins in wheat flour, these approaches are complicated by the genetic linkage of different classes of gluten protein genes and the finding that omega-5 gliadins may be encoded on more than one chromosome.

PBM-46

Proteomic Profiling and Epitope Analysis of the Complex α-, γ-, and ω-Gliadin Families in a Korean Wheat Variety “Keumkang”

Sewon Kim1, Kyoungwon Cho2, Susan B Altenbach3, Sun-Hyung Lim1, Jong-Yeol Lee1*

1National Institute of Agricultural Science, RDA, Jeonju 54874, Korea, 2Department of Biotechnology, College of Agriculture and Life Sciences, Chonnam National University,

Gwangju 61186, Korea, 3USDA-ARS, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710, USA

Wheat gliadins are a complex group of proteins that contribute to the functional properties of wheat flour doughs and contain epitopes that are relevant for celiac disease (CD) and wheat-dependent exercise-induced anaphylaxis (WDEIA). In this study, we extracted ethanol-soluble gliadin fractions from flour of the Korean bread wheat cultivar Keumkang. Proteins were separated by 2-dimensional gel electrophoresis (2-DE) using a pI range of 6– 11 in the first dimension and subjected to tandem mass spectrometry. α-, γ-, and ω-gliadins were identified as the predominant proteins in 31, 28, and one 2-DE spot, respectively. An additional six ω-gliadins were identified in a separate experiment in which a pI range of 3– 11 was used for protein separation. We analyzed the composition of CD- and WDEIA-relevant epitopes in the gliadin sequences from Keumkang flour, demonstrating the immunogenic potential of this cultivar. Detailed knowledge about the complement of gliadins accumulated in Keumkang flour provides the background necessary to devise either breeding or biotechnology strategies to improve the functional properties and reduce the adverse health effects of the flour.


184

PBM-52

LegExpress: An interactive bioinformatic platform for cross-species translation of transcriptome data in legumes

Joo-Seok Park1, Jin-Hyun Kim2, Yoram Choi1, Min-Gyun Jeong1, Hong-Kyu Choi3*1Department of Applied Bioscience, Dong-A University, 2Department of Medical Bioscience,

Dong-A University, 3Department of Molecular Genetics, Dong-A University

Genome-wide transcriptome analysis is one of the most powerful means to gain a broad and deep insight into the molecular mechanisms that underlie dynamic interactions among numerous genes in organisms. Although several bioinformatic platforms for gene expression profiling have been developed for individual species, platform for cross-species transcriptome analysis is not currently available. We employed the technical concept of translational genomics between different species and aimed to build the platform in user-friendly manner. This DB-linked platform, named LegExpress, harbors wide array of transcriptome data for three representative species with relatively the most comprehensive gene expression information, including Glycine max, Medicago truncatula and Arabidopsis thaliana. All these expression data were collected from publically available ArrayExpress (http://www.ebi.ac.uk/arrayexpress/) DB and composed mainly of Affymetrix GenChip data. Raw data were processed to select high-quality transcriptome data and normalized by the RMA standardization method. We developed a program for visualization of the data and organized the user interface according to suitable criteria, such as organs, developmental stages, time courses and different stimuli (e.g., hormones, biotic/abiotic stresses). It is anticipated that LegExpress may play a useful role for breeder/researcher-friendly transcriptome analysis platform and can be applied to design breeding programs through helping breeders discover trait-associated genes.

PBM-49

Cytoprotective effects of Centipedegrass extract against oxidative stress in NIH-3T3 fibroblasts

Dongho Bak1, Seong Hee Kang1, Kwangwoo Jung1, Kin Kyu Kim1,2, Byung Yeoup Chung1, Seung Sik Lee1,2*

1Research Division for Biotechnology, Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI), 2Department of Radiation Science

and Technology, University of Science and Technology (UST)

Centipedegrass originates from China and South America and is reported to contain several C-glycosyl flavones and phenolic constituents, including maysin and luteolin derivatives. This study aimed to investigate, for the first time, the anti-oxidative activity of Centipedegrass extract (CGE) and its potential molecular mechanism in fibroblast. Thus, the present investigation demonstrated that CGE, the potential antioxidative candidate to cope with the cellular oxidative damage. Our results showed that treatment of mouse fibroblast cells with CGE significantly inclined different anti-oxidative stress biomarkers such as SOD, CAT, GSS, and GPX1 following HO-1 transcription. In addition, ROS and TNF-α levels were also decreased upon CGE treatment and thereby, it induced cytoprotective effects. Amazingly, treatment of CGE significantly elevated cell survivability, by reversing all H2O2 induced alterations in fibroblast cells. Moreover, in exposure to the ionizing radiation-therapeutic application which is resulting in radiation dermatitis, the same protective effects observed. Therefore, our results indicated that, the CGE acted as a potential cytoprotective agent, which restored redox balance and cell homeostasis in cultured fibroblast cells, even after oxidative stress, suggesting that the candidate can be valuable as an effective remedy in treatment of oxidative stress related diseases and skin inflammation related disorders.

PBM-50

Acacetin stimulates GLUT4 translocation through insulin-independent pathway

Eun-Bin Kwon1,2, Myung-Ji Kang1,2, Hyung Won Ryu1, Seoghyen Lee3, Mi Kyeong Lee2, Su Ui Lee1, Mun-Ock Kim1*, Sei-Ryang Oh1*

1Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 2College of Pharmacy, Chungbuk National University,

3Department of Bioscience Biotechnology, Chungnam National University

Increasing the glucose utilization in insulin sensitive peripheral tissues such as adipose and skeletal muscle may help to control hyperglycemia associated with diabetes mellitus. We investigate whether acacetin promotes GLUT4-dependent glucose uptake in skeletal muscle cells, we performed [14C] 2-deoxy-D-glucose (2-DG) uptake assay after treatment of differentiated L6-GLUT4myc cells. Acacetin, a flavonoid compound, was isolated from Agastache rugosa. Acacetin dose-dependently increased 2-DG uptake and GLUT4 translocation into the plasma membrane. According to our results, acacetin activates the CaMKK-AMPK pathway by increasing intracellular calcium concentration. We also found that PKCζ/λ phosphorylation and intracellular reactive oxygen species (ROS) production were involved in the GLUT4 translocation promoted by acacetin. Moreover, acacetin suppresses oleic acid (OA)-induced lipid accumulation and de novo triglyceride through activation of AMPK in HepG2 cells. In addition, acacetin stimulated glucose uptake in HepG2 cells. Taken together, these results suggest that acacetin can be used as an antidiabetic functional ingredient and subsequent experiments using disease model animals are required.

PBM-51

Genome-wide Comparative Analysis of MicroRNAs and Disease-resistant Genes in Solanaceae Plants

Jeongyeon Yoon1, Taewook Kim1, Eunyoung Seo2, Taeyoung Um1, Taehyeon Park1, Doil Choi2, Chanseok Shin1*

1Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea, 2Department of Plant Science, Seoul National University, Seoul 08826,

Republic of Korea

MicroRNAs play roles in various biological processes like defense in plants. Some plant microRNAs produce phased, secondary siRNAs (phasiRNAs), which regulate cascade of gene expression. We investigated the relationship of Solanaceae microRNAs and defense genes in evolutionary perspective by performing genome-wide comparative analysis of microRNAs and their targets in Solanum plants. Degradome analysis showed that many of genes related to defense response are regulated by microRNAs in Solanum plants. We found that novel pepper microRNAs targeted genes encoding nucleotide-binding leucine rich repeat or receptor-like protein, known as disease-resistant genes. In addition, these novel microRNAs triggered phasiRNA production indicating amplification of regulation of the disease-resistant gene families. Taken together, microRNAs might be generated and evolved to regulate diverse genes involved in plant immunity. This study provides an insight into the evolution of novel microRNAs targeting plant defense genes and possible co-evolution with their target genes.This work was supported by the Next-Generation BioGreen 21 Program (No. PJ01333001), Rural Development Administration, Republic of Korea.

Poster Presentation


Poster Presentation

PBM-55

Ginseng-derived two CYP genes functions on plant growth and phenylurea herbicide tolerance

Jin Hoon Jang, Soo Kwang Yim, Ok Ran Lee*

Department of Applied Plant Science, College of Agriculture and Life Science, Chonnam National University

Cytochrome P450 enzymes (CYPs) are a superfamily of monooxygenases that are found in all living organisms. They represent extraordinary diversity in their reaction chemistry. Beside their physiological functions in hormone biosynthesis, lipid, and secondary metabolites, P450 enzymes function to tolerate harmful exogenous chemicals such as herbicides. Two CYP genes from ginseng, PgCYP736A12 and PgCYP76B93 were highly expressed in the root, rhizome, and leaves of ginseng. The expression level of the two CYP genes was regulated against a variety of abiotic stresses such as abscisic acid, chilling, chlorotoluron, hydrogen peroxide, jasmonic acid, NaCl and salicylic acid. Heterologous overexpressed two CYP genes in Arabidopsis shows reduced plant height and resistance to phenylurea herbicide, chlorotoluron. It indicates that the functional roles of two ginseng CYP genes in plant growth and herbicide tolerance. Ginseng as a perennial plant offers more sustainable solutions to herbicide resistance. Therefore, this function can be used as an important agricultural trait in the breeding of crops.

PBM-56

Involvement of chalcone synthase genes in the rice phytoalexin biosynthesis

Kyungha Lee, Hye Lin Park, Seong Hee Bhoo, Man-Ho Cho*

Graduate School of Biotechnology, Kyung Hee University

Phytoalexins are aitnmicrobial secondary metabolites in plants. Rice produces diverse phenolic and terpenoid phytoalexins. The flavonoid sakuranetin is well known phenolic phytoalexin in rice. In the phenolic phytoalexin biosynthesis, chalcone synthase (CHS) is an essential enzyme connecting the phenylpropanoid pathway and flavonoid pathway. Rice contains 33 CHS genes, collectively called OsCHSs. To delineate the role of OsCHS genes in the phytoalexin biosynthesis, we performed biochemical and mutant analyses. OsCHSs were clustered into two groups in the phylogenetic analysis, and OsCHS8 and 24 were classified with the functional CHS genes in other plant species. Twelve CHS genes were cloned from rice leaves and expressed in Escherichia coli. The recombinant CHS enzymes were purified and functionally characterized in vitro. Recombinant OsCHS8 and 24 were biochemically active catalyzing the stepwise condensation of p-coumaroyl-CoA and malonyl-CoA to naringenin chalcone. Quantitative real-time PCR and microarray data analyses showed that OsCHS8 and 24 genes were up-regulated in response to various biotic and abiotic stresses. The sakuranetin accumulation in OsCHS mutants under UV-stress conditions were also analyzed.

PBM-53

Identification of Damulin B in vitro metabolism using metabolomics technology

Ga-Hyun Lee1, Tae Lin Huh2, Kwang-Hyeon Liu1*1BK21 Plus Multi-Omics Based Creative Drug Research Team, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University,

Daegu 41566, Korea, 2School of Life Science and Biotechnology, Kyungpook National University, Daegu 41566, Korea

One of the saponins of Gynostemma pentaphyllum (GP), Gypenoside LV1 is metabolized to Damulin, which strongly activates AMP active protein kinase (AMPK). Damulin also increased β-oxidation and glucose uptake with increasing GluT4 (glucose transporter 4) translocation to the plasma membrane. In this study, we performed drug metabolism study using human liver microsomes. Damulin was metabolically stable in human liver microsomal incubation (T1/2>60 min). It was also stable in human plasma. Damulin had weak inhibitory potential against CYP2B6 activity (IC50=20.0 μM), whereas it had no inhibitory potential for CYPs 1A2, 2A6, 2C8, 2C9, 2C19, 2D6, 2E1, and 3A and UGTs 1A1, 1A3, 1A4, 1A6, 1A9, and 2B7. To elucidate the metabolic pathway of damulin, HLMs were incubated with damulin in the presence of UDGPA and NADPH. Seven metabolites (M1~M7) were found, and they were identified as hydroxy metabolites based on liquid chromatography-tandem mass spectrometric analysis (LC-MS/MS). We also performed damulin metabolism in HLMs using a non-targeted metabolomics approach and identified one novel metabolic pathway including hydrogenation and oxidation. The present data suggested that a non-targeted metabolomics approach will be good platform for the metabolite identification of xenobiotics. This research was supported by a grant of the National Research Foundation of Korea, Ministry of Science and ICT [NRF-2019R1A2C1008713], Republic of Korea.

PBM-54

LegCompara: a real time-responsible bioinformatic platform for the comparative genome analysis of legume genomes

Jin-Hyun Kim1, Joo-Seok Park2, Yoram Choi2, Min-Gyun Jeong2, Hong-Kyu Choi3*1Department of Medical Bioscience, Dong-A University, 2Department of Applied Bioscience,


Comparative genome analysis is a powerful approach to look into the genomic organizations among different, but evolutionary related, species, to predict function of certain genes of interest and to interpret evolutionary relationships between compared species. For such reasons, development of efficient and automated bioinformatic visualization tool is essential in this research field. To achieve this goal, we intended to construct an interactive and flexible bioinformatic interface for the comparative analysis focused on legume genomes, named ‘LegCompara’. This platform consists mainly of two parts: a web-based user interface and corresponding relational databases. The database harbors a diverse array of genomic information (e.g., functional annotation, ortholog groups) for seven legumes (M. truncatula, G. max P. vulgaris, C. cajan, V. radiata, C. arietinum) and two model plants (A. thaliana, O. sativa). This genome browser, unlike other traditional genome browsers, was designed for researchers to dynamically interact with user interface, so it can navigate multiple chromosomes of different or same species simultaneously, resulting in genome-wide and/or regional comparisons by depicting corresponding syntenies with either blocks or lines between orthologous regions or genes. It is expected that LegCompara may provide researchers and breeders with useful resources for more efficient and user-friendly comparative genome analysis.


186

PBM-60

Effect of S219V mutation on activity of Tobacco Etch Virus (TEV) protease

Heejin Nam1, Sooim Shin1,2*1Interdisciplinary Program of Bioenergy and Biomaterials Graduate School, College of

Engineering, Chonnam National University, 2Department of Biotechnology and Bioengineering, College of Engineering, Chonnam National University

Tobacco Etch Virus is the major genus of the Potyvirus, and has approximately 9.5 kb ssRNA in its genome. The RNA genome is translated to the polyprotein called Nuclear Inclusion a (Nia) which contains a catalytic domain at 27 kDa domain. The catalytic triad residues of the active site are His46, Asp81, and Cys151 located at the interface of the domain. This domain recognizes a specific amino acid sequence, ENLYFQG/S, and cleaves between Q and G/S. Despite substrate specificity of the catalytic domain, the protease has some limitations to utilize it in experiment; inhibition of the activity by self-cleavage and poor solubility during purification. In here, we isolated the S219V mutant TEV protease in the background of T17S, N68D and I77V mutations in soluble expression level. Also, the activity of the isolated mutant was measured by SDS-PAGE and the fluorescence assay. It was confirmed in gel electrophoresis that the substrate containing the TEV protease recognition site was clearly truncated by the isolated mutant TEV protease. As a result of steady-state kinetics, 2.8×10-2 ±1.52×10-3 nM.min-1 of kcat the new mutant was obtained and it was 30-fold faster than the commercially purchased one. These results indicate that the activity and solubility was enhanced by multiple mutations on TEV protease.

PBM-57

Genome-wide association study dissects seed pigmentation-associated genes and loci in soybean genome

Jin-Hyun Kim1, Joo-Seok Park2, Yoram Choi2, Min-Gyun Jeong2, Hong-Kyu Choi3*1Department of Medical Bioscience, Dong-A University, 2Department of Applied Bioscience,


Soybean (Glycine max [L.] Merr.) is the most important crop among all cultivated legume crops in the world. Seed coat has been significantly influenced during the period of domestication in that it is linked to seed dormancy, seed viability and cost factors in processing seeds for oil and soy foods. To identify loci linked with seed pigmentation, we collected the resequencing data of 438 accessions. A genome wide association study using all possible combinations of three traits revealed four loci (designated as SP1-SP4). More important, we identified a gain of function mutation affecting a CaaX-type endopeptidase gene (Glyma.01G198500), which was a chloroplast-targeted transmembrane protein, as a strong candidate for the green seed coat. Glyma.01G198500 gene was highly coexpressed with the genes associated with chloroplast development and shared CaaX protease self-immunity domain (PF02517) with SCO4 which is a chloroplast-targeted protein that plays important roles in development of chloroplast. Glyma.01G198500 protein of the green soybeans had all of the CaaX protease self-immunity domain and resembles alpha-helical bundle structure of major three transmembrane protein structures, whereas that of the yellow soybeans had a partial CaaX protease self-immunity domain and was far from the alpha-helical bundle structure. This study provides insights into how to effectively utilize the data accumulated in the public databases and the interaction of four loci controlling seed pigmentation.

PBM-58

Insight about variations in multiple copies of bacterial 16S rRNA genes may aid in species identification

Jerald Conrad Ibal, Chang Eon Park, Yeong-Jun Park, Min-Chul Kim, Young Jae Jo, Huy Quang Pham, Gi-Ung Kang, Sajjad Ahmad, Jae-Ho Shin*


Variable region analysis of 16S rRNA gene sequences is the most common tool in bacterial taxonomic studies. Although used for distinguishing bacterial species, it remains limited due to the presence of variable copy numbers with sequence variation in the genomes. 16S rRNA gene sequences obtained from whole genome sequencing and Sanger electrophoresis sequencing of cloned PCR products from Serratia fonticola GS2, were compared. Sanger sequencing produced a combination of sequences from multicopies of 16S rRNA genes. To determine whether the variant copies of 16S rRNA genes affected Sanger sequencing, two ratios (5:5 and 8:2) with different concentrations of cloned 16S rRNA genes were used; it was observed that the greater the number of copies with similar sequences the higher its chance of amplification. Effect of multicopies for taxonomic classification of 16S rRNA gene sequences was investigated. 16S rRNA copies with the maxiumum variation had 99.42 % minimum pairwise similarity and did not have an effect on species identification. PCR products from genomes containing variable 16S rRNA gene copies can provide sufficient information for species identification except from species which have high similarity of sequences in their 16S rRNA gene copies like the case of Bacillus thuringiensis and Bacillus cereus. Overall, our results show how variations in the multicopies of the 16S rRNA genes of bacteria can aid in appropriate species identification.

PBM-59

Copper incorporation energetics in amicyanin’s folding mechanism

Seounghun Jeoung1, Kyungil Bang2, Sooim Shin1,2*, Moonsung Choi3*1Interdisciplinary Program of Bioenergy and Biomaterials Graduate School, College of

Engineering, Chonnam National University, 2Department of Bioengineering and Biotechnology, College of Engineering, Chonnam National University, 3Department of Optometry, College of Energy and Biotechnology, Seoul National University of Science

and Technology

Cofactors in proteins participate mainly in biological activities that include oxygen transport, electron transfer, and catalysis. Beside these activities, cofactors play a role in protein folding. Amicyanin has a single copper in their active site as a cofactor and mediates the electron transfer reaction between methylamine dehydrogenase (MADH) and cytochrome c-551i for energy production in Paracoccus denitrificans. In this study, the rates and thermodynamic parameters of copper incorporation into two different folding states of amicyanin; fully folded apo-amicyanin and partially folded apo-amicyanin, were measured to propose the overall kinetic mechanism for amicyanin biogenesis. When the incorporating copper into partially folded apo-amicyanin, bi-phasic kinetics was observed unlike fully folded apo-amicyanin. The rate for the incorporation of copper into fully folded apo-amicyanin was almost 9-fold slower than that for the initial phase of partially folded apo-amicyanin. Thermodynamic parameters of incorporating copper into fully folded and partially folded apo-amicyanin, were measured by Van’t Hoff equation and isothermal titration calorimetry (ITC). Value of ∆H from partially folded apo-amicyanin was less than that from fully folded apo-amicyanin in both experiments. These results demonstrate that during amicyanin biogenesis copper rapidly binds to apo-amicyanin before protein folding and then it gives pivot for amicyanin folding influencing the remaining optimization for the completion of the protein folding reaction.

Poster Presentation


Poster Presentation

PBM-63

Structural Insights and Kinetic Characterization of MICAL3: Comparison to MICAL1 by their CH Domains

Yeon Jin Roh, Byung Cheon Lee*

College of Life Sciences & Biotechnology, Korea University, Seoul 02841, Republic of Korea

The molecules interacting with CasL (MICALs) are large multidomain proteins that participate in cytoskeletal dynamics via actin disassembly. In the presence of NADPH, MICALs mediate actin depolymerization by oxidation of conserved methionine residues in D-loop connecting actin monomers in F-actin. Mammals have three isoforms of MICAL, MICAL1, 2 and 3, and all isoforms contain the N-terminal flavin-containing monooxygenase (FMO) and Calponin-Homology (CH) domains. We identified the structure of truncated MICAL3 that comprise FMO and CH domains and superimposed the structure of MICAL1 and MICAL3. We found that the actin binding CH domains of MICAL1 and MICAL3 have different position in an asymmetric unit and anticipated that the distinction of the CH domain would bring some discrepancy to the enzymatic activity of each MICALs. We here, to verifying the importance of CH domain in MICAL activity, generated chimeric MICALs that swapped the CH domains between MICAL1 and MICAL3 and compared the kinetic parameters of each MICAL forms (MICAL1, MICAL3 and chimeric MICALs). MICALs are crucial cytoskeletal regulators and their activities are important in early neural development and even cancer progression. This study will contribute to the understanding the intermolecular interaction between catalytic domain (FMO domain) and actin binding domain (CH domain) of MICALs in their catalytic activities and to identifying the specific target sites to modulate the oxydoreductase activity of MICALs.

PBM-64

Evalutaion of Leaf Rust Resistance and the characteristic in Korean wheat

Minseo Kim, Byung Cheon Lee*


Leaf rust is one of the major causes of reducing the yield of wheat. The Puccinia triticina causing leaf rust is an obligate parasite. In this study, we evaluate the Korean hexaploid wheat, Triticum aestivum L. (AABBDD genome, 2n=6x=42) about resistance to leaf rust in seedling stage. 40 kinds of Korean wheat were represented by 0 – 4 scale, and resistant and susceptible line were selected. Based on the screening results, the characteristics of selected cultivars were investigated by seed weight and chlorophyll assay.The expression of the relevant pathogenesis-related (PR) protein like β-1,3-glucanase known to be expressed when inoculated with leaf rust was confirmed. No significant difference was observed at 12, 24h after leaf rust inoculation. And, other characteristic like antioxidant activity by DPPH assay was evaluated in selected cultivars. This study provides important resources for the improvement of synthetic hexaploid wheat.

PBM-61

BRCA1 regulates RNA polymerase II promoter-proximal pause release through modulating topoisomerase IIb stability

Heeyoun Bunch*, Sanghwa Lee

Applied Biosciences, Kyungpook National University

Previously, we have shown that RNA polymerase II (Pol II) pause release in stimulus-inducible genes is accompanied with and requires DNA strand break and DNA damage response (DDR) signaling. This phenomenon involves topoisomerase IIb (TOPIIb)-mediated DNA double strand break (DSB), the activation of phosphatidylinositol 3-kinases (PI3K) such as ATM and DNA-PK, and a series of phosphorylation events of the substrates of these kinases including TRIM28 (S824) and H2AX (S139). Here, we report that BRCA1-BARD1 complex is involved in this process and essential for Pol II pause release in human immediate early genes (hIEGs). Our findings propose a novel role of BRCA1-BARD1 complex to modulate TOPIIb and Pol II pausing to control transcription.

PBM-62

Development of fluorescence-based biosensor using fRMsr for detecting accumulated damage by oxidative stress

Seahyun Kim, Hae Min Lee, Aro Lee, Bung Cheon Lee*


Reactive oxygen species are regulators of cellular functions, and over-produced ROS can be an indicator of various pathological processes. Methionine is reversibly oxidized to methionine sulfoxides by reactive oxygen species and thus could be a potential indicator to present redox status inside cells. Here we generated the thioredoxin (Trx)/cpYFP/free methionine-R-sulfoxide reductase (fRMsr)-based redox biosensor that detects a level of free methionine-R-sulfoxide (fMetRO) for monitoring variation of oxidative stress in real-time. A cpYFP was inserted between thioredoxin and fRMsr and its fluorescent value changes upon the formation of inter-disulfide bond between thioredoxin and fRMsr after the reduction of free methionine-R-sulfoxide by fRMsr. The reduced recombinant sensor showed two peaks of excitation with maxima at 430 nm and 500 nm. Reaction with a free MetRO-containing substrate and H2O2 led to increasing the excitation fluorescence ratio at 500 nm without change at 430 nm. By contrast, the addition of DTT and GSH induced a decrease in the fluorescence ratio. The sensor named TYf was used for analyzing fMetRO contents in cell lysate and monitoring fMetRO change and production at the single-cell level in cytoplasm, mitochondria, endoplasmic reticulum, and nucleus. In this study, we successfully created the biosensor using Trx1/cpYFP/fRMsr that works for monitoring change of oxidative stress. It has also been demonstrated its possibility in various applications.


188

PBM-68

Lapathoside A Isolated from Fagopyrum esculentum Induces Cell Death in Human Pancreatic Cancer Cells

Mi Sook Kang1, Young-Min Ham2, Song-I Han3, Dae-Ju Oh2, Yong-Hwan Jung2, Ui Hyeon Oh1, Da-Hye Kim1, Cheol Lee1, Dahye Jeong1, Jae Hoon Kim1,3*

1Department of Biotechnology, College of Applied Life Science, Jeju National University, 2Jeju Biodiversity Research Institute, Jeju Technopark, 3Subtropical/Tropical Organism

Gene Bank, Jeju National University

Buckwheat, Fagopyrum esculentum, is a common pseudo-cereal in Asia like China, Korea, Japan, and Europe. Its seeds are containing not only many complex carbohydrates but also protein, minerals, polyphenols, and aromatic compounds. In the present study, lapathoside A, a phenylpropanoid ester compound, was isolated from the roots of buckwheat. 70% Ethanol extract of buckwheat roots was separated into n-hexane, methylene chloride, ethyl acetate, n-butanol, and water fractions by solvent partitioning. Seven fractions were obtained from ethyl acetate fraction by liquid chromatograph and fraction No. 6 was containing lapathoside A. The structure of lapathoside A was determined by LC-MS, LC-MS/MS, and NMR analysis. We performed cell viability assay to show the cytotoxic effect of lapathoside A in human pancreatic cancer cell lines (PANC-1 and SNU-213). After treatment of lapathoside A, cell viability of PANC-1 and SNU-213 cells decreased to 40% and 30% at 25 μM. This result indicated that lapathoside A has an antitumor effect in human pancreatic cancer cell lines. Therefore, further studies are needed to confirm whether it is associated with cell apoptosis signaling pathway.

PBM-65

Comparison of diosgenin related gene expression and content on organs of Trigonella foenum-graecum L.

Do Yeon Kwon1, Sang Un Park2*1Smart Farm Resesarch Center, KIST Gangneung Institute of Natural Products,

2Department of Crop Science, Chungnam National University

Diosgenin is belong to the group of triterpenes that is a naturally major bioactive occurring steroid sapogenin and present in Dioscorea species (yams), fenugreek and Cotus sepciosus etc. This compound used pharmaceutical industry as the main precursor such as anti-inflammatory, lipid metabolism and anti-diabetes that is main bioactive chemical in Fenugreek. In order to compare diosgenin contents, different organs of fenugreek harvested, were examined in this study. Different organs (the flowers, stems, young leaves, mature leaves, roots and pods) were prepared, and the gene expression related diiosgenin and diosgenin accumulation were analyzed. Gene epxression shown highest in flower and then followed roots. In case of pod, all gene expression were shown very low. The highest accumulation was in young leave (2.05 mg/g), followed flower (0.79 mg/g), mature leave (0.79 mg/g) and pod (0.71 mg/g) that the most accumulated organ and the next organ were more than half. The lowest content level was in stem (0.17 mg/g) and root (0.4 mg/g).

PBM-66

5’, 8”-Biapigenin is selective CYP2C8 and CYP2C9 inhibitor in human liver microsomes

So-Young Park*, Su-Nyeong Jang, Kwang-Hyeon Liu

BK21 Plus KNU Multi-Omics Based Creative Drug Research Team, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National,

Kyungpook National University, Daegu 41566, Korea

Biapigenin is a biologically active compound with neuroprotective and anti-inflammatory activity, which is isolated from the Selaginella tamariscina. In this study, we evaluated the inhibitory potential of biapigenin against cytochrome P450 (P450) and UDP-glucuronosyltransferase (UGT) activities in human liver microsomes (HLMs) using cocktail incubation and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Biapigenin noncompetitively inhibited CYP2C8-mediated amodiaquine N-deethylation and rosiglitazone N-demethylation activities with Ki values of 0.018 and 0.010 µM, respectively. It also competitively inhibited CYP2C9-mediated diclofenac hydroxylation with a Ki value of 0.032 µM. Biapigenin, however, relatively weakly inhibited CYP1A2, CYP2B6, and CYP3A with Ki values of 3.1, 7.9, and 4.5 µM, respectively. The inhibitory potential of biapiganin for CYP2A6, CYP2C19, CYP2D6, and CYP2E1 were ignorable (IC50>10 µM). Biapigenin has selective inhibitory effect on CYP2C8 and CYP2C9 isoforms compared to other seven P450 isoforms (Selectivity index>24.9). Therefore, biapigenin might be used as a selective inhibitor of CYP2C8 and CYP2C9 enzymes in P450 phenotyping study. This research was supported by a grant of the National Research Foundation of Korea, Ministry of Science and ICT [NRF-2019R1A2C1008713], Republic of Korea.

PBM-67

Enhancement of Production of High-value Benzaldehyde Chemicals from Lignocellulosic Biomass by

Thermoalkaliphilic Laccase, CtLac

Youri Yang, Sunil Ghatge, Woo-Young Song, Tae-Young Kim*, Hor-Gil Hur*

School of Earth Sciences and Environmental Engineering, Gwangju Institute of Science and Technology (GIST)

Enzymatic conversion of lignin into high-value chemicals is a key step in sustainable and eco-friendly development of lignin valorization strategies. In the present study, a novel thermoalkaliphilic laccase, CtLac, from Caldalkalibacillus thermarum strain TA2. A1 was tested for the depolymerization of lignin and the production of value-added chemicals, using three different lignocellulosic biomass, organosolv lignin (OSL), and Kraft lignin. Seven valuable lignin monomers were identified from the CtLac-treated samples using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Remarkably, increases of 22.0%, 65.6%, and 27.3% of p-hydroxybenzaldehyde and increases of 111.1%, 93.5%, and 238.1% of vanillin were observed from rice straw, corn stover, and reed, respectively. Comparative analysis of lignin monomers released from rice straw, using Trametes versicolor laccase (TvL) and CtLac indicated efficient depolymerization of lignin by CtLac. CtLac treatment resulted in 2.3 fold and 5.6 fold, and 1.9 fold and 2.8 fold higher amounts of p-hydroxybenzaldehyde and vanillin from OSL and Kraft lignin, respectively, compared to CtLac-treated rice straws amples after 12 h reaction. OSL was the best substrate for the production of benzaldehyde chemicals using CtLac treatment. The results demonstrated potential application of bacterial laccase CtLac for valorization of biomass lignin into high-value benzaldehyde chemicals under thermoalkaliphilic conditions.

Poster Presentation


Poster Presentation

PBM-71

The neuroprotective potentiation of the ethanol extract from Styrax japonica in HT22 mouse hippocampal cells

Da Hye Jeong1, So-Mi Kang1,2, Song-I Han3, Da-Hye Kim1, Ui Hyeon Oh1, Cheol Lee1, Mi Sook Kang1, Jae-Hoon Kim1,3*

1Department of Biotechnology, College of Applied Life Science, Jeju National University, Jeju 63243, Republic of Korea, 2Quegen Biotech Co., Ltd., Jeju-Si, Jeju-Do, Korea, 3Subtropical/Tropical Organism Gene Bank, Jeju National University, Jeju 63243,

Republic of Korea

One of the early symptoms of Alzheimer’s disease is the dysfunction of cholinergic and glutamatergic synapses. Oxidative stress induced by excessive glutamate treatment causes neuronal degeneration and neurotoxicity. In this study, we found that the extract of Styrax japonica (S. Japonica) leaves (ESJ) inhibited apoptosis of HT22 mouse hippocampal cells from glutamate-induced cell death. First, we checked cell viability through MTT assay to check whether ESJ inhibits glutamate-induced cell death. We also used flow cytometry to analyze apoptosis of HT22 cells. Then, protein expression and phosphorylation levels of the mitogen-activated protein kinase (MAPK) pathways were evaluated by Western blot assays. In addition, we found that ESJ could inhibit acetylcholinesterase activity. As a result, we suggest that ESJ could contribute to the development of dementia drugs to enhance neuroprotection.

PBM-72

Eriodictyol induced apoptosis through the PI3K/Akt pathway in human pancreatic cancer cells

Ui-Hyeon Oh1, Jungwhoi Lee2, Song-I Han2, Da-Hye Kim1, Da Hye Jeong1, Cheol Lee1, Mi Sook Kang1, Jae Hoon Kim1,2*

1Department of Biotechnology, College of Applied Life Science, Jeju National University, 2Subtropical/Topical Organism Gene Bank, Jeju National University

Despite many studies over the past several decades, pancreatic cancer has known as one of the most vulnerable cancers. Apoptosis is considered one of the well-known treatment options for various cancer treatment research methods. Eriodictyol, a plant-derived flavanone found in citrus fruits has been known to have diverse biological activities such as anti-oxidant, anti-cancer, and anti-inflammatory properties. In this study, we have investigated the anticancer property of eriodictyol and its mechanisms of action in pancreatic cancer cells. Eriodictyol showed anticancer activity in PANC-1 and SNU213 cells but low cytotoxicity in the control cell. The induction of eriodictyol-induced apoptosis was confirmed by using annexin V-FITC/PI staining in both SNU213 and PANC-1 cells. Also, we found that eriodictyol induced apoptosis through PI3K/Akt signaling. These results can help to discover the molecular mechanisms of eriodictyol-induced cell death in pancreatic cancer cell lines and potentially contribute to the development of candidate reagents for pancreatic cancer.

PBM-69

2-Methoxy-4-vinylphenol attenuates migration of human pancreatic cancer cell via blocked of FAK and AKT signaling

Da-Hye Kim1, Jungwhoi Lee2, Boram Go3, Song-I Han2, Ui-Hyeon Oh1, Cheol Lee1, Da Hye Jeong1, Mi Sook Kang1, Jae Hoon Kim1,2*

1Department of Biotechnology, College of Applied Life Science, Jeju National University, 2Subtropical/Tropical Organism Gene Bank, Jeju National University,

3Jeju Biodiversity Research Institute, Jeju Technopark

Pancreatic cancer is the most risky cancer because of difficulty to diagnose and easily migration to other organs. So there is a need for research on the treatment agents of pancreatic cancer. 2-Methoxy-4-vinyl phenol (2M4V) is an aromatic compound that is mainly included in Juniperus chienesis. 2M4V is used as a flavoring agent and has various functions such as cell cycle arrest and anti-inflammatory. In this study, we confirmed 2M4V anti-cancer effects on pancreatic cancer cell lines (Panc-1 and SNU-213). 2M4V reduced viability of Panc-1 cells by inhibiting proliferation of cell nuclear antigen (PCNA) protein. 2M4V also inhibited the migratory activity of Panc-1 and SNU-213 cells. In addition, we verified the treatment of 2M4V effectively decreased the phosphorylation of FAK, and AKT pathway. These results suggested that 2M4V might be used in the development of pancreatic cancer treatments.

PBM-70

Effects of Toxicodendron succedaneum extracts on Differentiation and Adipogenesis in 3T3-L1 preadipocytes

Cheol Lee1, Ui Hyeon Oh1, Da Hye Jeong1, Da-Hye Kim1, So-Mi Kang1, Mi Sook Kang1, Song-I Han2, Jae Hoon Kim1,2*

1Department of Biotechnology, College of Applied Life Science, Jeju National University, 2Subtropical/ Tropical Organism Gene Bank, Jeju National University

Toxicodendron succedaneum has been used in indigenous medicine for the treatment of cough, asthma, and colicky pains and has been known for its effects on hangover, anti-tumor, anti-oxidation, and gastritis. In this study, we identified that the extract of methanol of T. succedaneum has and anti-oxidant effect and an inhibitory effect on adipocyte differentiation and adipogenesis. T. succedaneum extract was found to have high pancreatic lipase inhibition activity along with Limonium tetragonum and Diospyros lotus. The DPPH radical scavenging assay showed that the antioxidant activity of T. succedaneum extract higher than Limonium tetragonum and Diospyros lotus extracts. Also, we measured MTT assay to measure the toxicity of T. succedaneum extract and performed Oil red O assay to see how much lipid droplets were inhibited. As a result, T. succedaneum extract effectively inhibited lipid accumulation in 3T3-L1 cell line. In conclusion, these results are expected to contribute to the reduction of obesity-related diseases by being utilized in the food and pharmaceutical industries.


190

PBM-76

Establishment of Extract Method for Mepiquat chloride in Paprika and Potato with LC-MS/MS

Min-Seok Oh1*, Sujin Baek1, Hyun Ho Noh1, A. K. M. Mydul Islam1, Jin-Ho Ro1, Danbi Kim1, Hyeyoung Kwon2, Byeong-Chul Moon1

1Department of Agro-food Safety, National Institute of Agriculture Sciences, Rural Development Administration, 2Planing and Coordination Bureau,

Rural Development Agency

This study carried out to determine analysis method for the pesticide residue of mepiquat chloride that is widely used as plant growth regulator in paprika and potato. In order to establish analysis method more simple and correct, sample preparation process was performed only extraction. Electrospray ionization mode was selected by positive and MRM mode considering for sensitivity and selectivity of instrumental analysis. The limit of quantitation (LOQ) of analysis method and linear equation of calibration curve was 0.01 mg/kg and more than 0.99. To establish extraction method, samples were extracted with methanol and then those were centrifuged at 3,500 rpm for 5 minute. Extracted samples were analyzed for calculating recovery. As results, average recoveries of mepiquat chloride in paprika and potato were 127.80 and 131.34, respectively, and their RSD was less than 1.3% in all samples. It was concluded that this was not suitable for analysis. Because it exceeds the effective recovery range of 70-120%. To complement this, acetonitrile was used for extraction of mepiquat chloride in samples. QuEChERS kit called by EN method was added for separation both water and organic solvent layer and then those were centrifuged at 3,500 rpm for 5 minute. As results, recoveries of test pesticide found to be 78.63% in paprika and 81.98% in potato. Also, relative standard deviation was less than 1.9%.

PBM-73

Identification of Two Cytochrome P450 Genes Involved in 20-Hydroxyecdysone Biosynthesis of Spinach

(Spinacia oleracea L.)

Viet Dang Cao1,2, Jiwon Kim1, Key-Zung Riu1, Chang Sook Kim1, Kyung-Hwan Boo1,2*

1Department of Biotechnology, College of Applied Life Science (SARI), Jeju National University, Jeju 63243, Republic of Korea, 2Subtropical/Tropical Organism Gene Bank,

Jeju National University, Jeju 63243, Republic of Korea

20-Hydroxyecdysone (20E) is the major phytoecdysteroid and plays a defensive role in plant to prevent feeding by phytophagous insect. However, very little is known about 20E biosynthetic gene in plant, in contrast to insect whose six genes including five cytochrome P450 (CYP) genes have been identified. Only one CYP71 family gene (CYP71D443) in Ajuga reptans has been identified to catalyze the conversion of 5β-ketone to (22R)-hydroxy-5β-ketone which is an intermediate step in 20E biosynthetic pathways. Therefore, in this study we tried to identify CYP71 family genes involved in 20E biosynthesis of spinach (Spinacia oleracea L.), a unique vegetable producing high yield of 20E. Functional analyses of seven candidate genes isolated from spinach were performed by loss- and/or gain-of-function using virus-induced gene silencing (VIGS) and transient gene over-expression systems (TGOE), respectively. As a result, expression levels of two genes controlled by VIGS and TGOE were highly correlated to 20E concentrations, indicating that these two genes might be involved in 20E biosynthesis of spinach. These results could be useful not only for understanding 20E biosynthesis in spinach but also for breeding insect resistant crops based on 20E biosynthesis.

PBM-74

Pathogenic Characters of Pseudomonas tolaasii Strains can be Changed during the Obtaining Phage Resistance

Yeong-Bae Yun, Young-Kee Kim*

Department of Environmental and Biological Chemistry, Chungbuk National University

Pseudomonas tolaasii (Pt) is a pathogenic bacteria causing brown blotch disease on the cultivated mushrooms. We have been isolated various pathogenic P. tolaasii strains and they were divided into three subtypes, Ptα, Ptβ, and Ptγ. To control of the disease, phage therapy has been performed and the phage-resistant mutant (PRM) strains have been characterized because of their frequent occurrence. Ten PRM strains were induced against phage Φ6b31 from P. tolaasii 6264, the Ptα type parent strain. Seven out of 10 PRM strains showed a pathogenic character of Ptβ type and three strains were Ptα type. In this study, the eleven 2nd

PRM strains were induced from Ptβ type, P. tolaasii 1R strain (1st PRM strain of P. tolaasii 6264 against Φ6b1) against phage Φ6b7. In the phylogenetic analyses, eight 2nd PRM strains were identical to the P. tolaasii 6264 in the 16S rRNA gene sequences. Various pathogenic characters of the 2nd PRM strains were measured and compared with those of the parent strain. Nine out of 11 2nd PRM strains showed changed pathogenic characters of Ptα type from those of Ptβ type. Two PRM strains remained the same Ptβ type, forming transparent colonies and losing hemolytic activity. These results suggest that the pathogenic characters could be changed during the acquisition of phage-resistance, from Ptα type to Ptβ type, or vice versa.

PBM-75

Assessment of Hfq-dependent sRNAs on the expressions of individual multidrug-efflux pump in Escherichia coli

Yun Son, Kwang-Sun Kim*


Infections arising from multidrug-resistant pathogens are rapidly spreading throughout the world and threaten to become untreatable by current antibiotics. Moreover, the number of people who die from bacterial infections is expected to increase dramatically over those who die from cancer in year 2050. Under these circumstances, it is of great significance to identify mechanisms that cause antibiotic resistance. Among the known mechanisms, multidrug efflux pumps (MDEPs), bacterial systems involved in the drug export, is well known. Especially, 21 MDEP genes have been identified in Escherichia coli, but little is known underlying factors by which the expression of MDEP genes are regulated in post-transcriptional level. Hfq-dependent small RNAs (sRNAs) have been regarded as the major regulators of gene expression at post-transcriptional level. In this study, we performed the screening to identify sRNAs involved in the MDEP gene expression using reporter systems in which the promoter of individual genes is fused to LacZ in the sRNA overexpression library. Analysis revealed that multiple sRNAs were involved extensively in the regulation of the expression of MDEPs and therefore, we expected that sRNAs could constitute a major part of regulators to control MDEP-associated antibiotic resistance.

Poster Presentation


Poster Presentation

PBM-79

Characterization of PtrGATA33 related to secondary cell wall biosynthesis in Arabidopsis

Jooeun Lee, Hyeonjung Jung, Won-Chan Kim*


The GATA family is widely distributed in eukaryotes, including fungi, metazoans, and plants. Numerous GATA family members contain a single C-X2-C-X17-19-C-X2-C domain that is important in light response. In plants, GATA transcription factors also have crucial roles in seed germination, cytokinin response, flower development and chloroplast development. However, only a few studies on Poplar GATA transcription factor have been reported. Here, we tried to find out function of Poplar trichocarpa GATA33 (PtrGATA33). PtrGATA33 was identified as the homologous gene of Arabidopsis GATA25. In previous studies, AtGATA25 was named as ZIM for Zinc-finger protein expressed in Inflorescence Meristem. ZIM was affected by light, circadian regulation and involved in hypocotyl, petiole elongation. Both ZIM and PtrGATA33 overexpression plants showed altered cell elongation in hypocotyl as previously reported. PtrGATA33 overexpression plants showed that supple stem than control (wild type), making it easier to bend. RT-PCR result revealed that secondary cell wall related gene expression was reduced and the secondary cell wall of stem appeared to be thinned in PtrGATA33 overexpression plants. While SRDX plants, cell wall thickening in phloem fiber were observed. Based on these results, PtrGATA33 may act in secondary cell wall biosynthesis as well as elongation.

PBM-80

PtrGATA18 regulates master regulator of secondary cell wall biosynthesis in Arabidopsis thaliana

A-Hyeon Kang, Ki Hwan Lim, Ki Hwan Kim, Won-Chan Kim*


In vascular plants, secondary cell walls are formed in specific types of plant cells, which provide mechanical strength and transport water and nutrients for growth and development of the plants. Secondary cell walls consist of cellulose, hemicellulose, and lignin. These polymers are formed by secondary cell wall biosynthesis pathway involved various genes. Transcription factors known as master regulators control at the top of this pathway. However, pathway about upstream of master regulators is unclear. In this study, we identified Populus thricocarpa GATA transcription factor 18 (PtrGATA18) influences secondary cell walls biosynthesis by interacting with master regulators. Overexpression of PtrGATA18 in Arabidopsis thaliana reveal a dwarf phenotype and cell wall thickening in the stem. We demonstrated that PtrGATA18 regulates secondary cell walls formation by confirming the expression level of secondary cell wall related genes. The expression level of secondary cell walls biosynthesis genes including master regulators are up-regulated in PtrGATA18 ox plant compared to wild type. Furthermore, we confirmed PtrGATA18 and A. thaliana GATA transcription factor 9 (AtGATA9) bind to the promoter of secondary cell walls biosynthesis genes. In conclusion, we proved that PtrGATA18 regulates expression of master regulators and induces secondary cell walls thickening in A. thaliana.

PBM-77

Homology-directed repair (HDR)-based genome editing in tomato

Jae-Yean Kim1*, Tien Van Vu1, Jihae Kim2, Doan Thi Hai Duong2

1Division of Applied Life Science (BK21 Plus program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 2Division of Applied

Life Science (BK21 Plus Program), Gyeongsang National University

Genome editing (GE) based on CRISPR technology, a technique coined as plant breeding innovation (PBI), is expected to open a new era of crop breeding. GE takes place usually through the DNA repair pathways. DNA double strand breaks (DSBs) occurred in cells are a serious threat to survival and all living organisms have developed mechanisms to treat DSB rapidly. Among them, cells rapidly ligate the ends of the truncated DNA through the non-homologous end joining (NHEJ) repair pathway, where some nucleotides can be deleted or inserted, resulting in mutations. On the other hand, homology-directed repair pathway can occur when homologous DNA templates are present, which is very inefficient compared to the NHEJ pathway in plant somatic cells and is not well utilized as a tool in plant GE. Unlike the NHEJ repair pathway, which mainly produces knock-out, the HDR pathway can replace long DNA fragments as well as SNP mutations, allowing replacement of alleles or pyramidal integration of multiple genes in a specific locus of a chromosome. The latter is very advantageous to crop breeding. We report our approaches and results to improve HDR efficiency through various approaches using geminivirus - based plant replicon in tomato model crops.

PBM-78

Ginsenoside Rc is a new selective UGT1A9 inhibitor in human liver microsomes and recombinant human UGT isoforms

Su-Nyeong Jang*, Hyunyoung Lee, Jae-Kyung Heo, Ga-Hyun Lee, So-Young Park, Hyun-Ji Kim, Kwang-Hyeon Liu

BK21 Plus KNU Multi-Omics Based Creative Drug Research Team, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University,

Daegu 41566, South Korea

In vitro investigation of the inhibition of ginsenosides towards UGT1A9 was carried out using human liver microsomes (HLMs). Among ten ginsenosides, ginsenoside Rc showed the strongest inhibition against UGT1A9-mediated mycophenolic acid glucuronidase activity. The further inhibition kinetic studies using HLMs suggested that ginsenoside Rc competitively and noncompetitively inhibited the UGT1A9-mediated propofol and mycophenolic acid glucuronidation activities with Ki values of 2.83 and 3.31 mM, respectively. In order to investigate whether the inhibitory effect of ginsenoside Rc is selective to UGT1A9 isoform, we studied the inhibitory potency of ginsenoside Rc on nine human UGT activities using recombinant human UGT isoforms. Ginsenoside Rc exhibited 12.9-fold selectivity similar to niflumic acid (12.5-fold) for UGT1A9 inhibition. Ginsenoside Rc at 50 mM concentration inhibited none of the other UGT isoform-specific activities above 12.0%, except for UGT1A9 (>91.5%) in HLMs, indicating that ginsenoside Rc might be used as UGT1A9 selective inhibitor in reaction phenotyping study of new chemical entities. In addition, ginsenoside Rc has the potential to cause pharmacokinetic drug interactions with other co-administered drugs metabolized by UGT1A9. However, given that the varied contribution of these UGT isoform towards drug metabolism and the influence of ginseng complexity and individual difference, the explanation of these results should be paid more caution. This research was supported by a grant of the National Research Foundation of Korea, Ministry of Science and ICT [NRF-2019R1A2C1008713], Republic of Korea.


192

PBM-84

A TMT-based proteome analysis of plasma membrane and cytosolic fractions highlight the MSP1 induced signaling

in rice leaves

Ravi Gupta, Cheol Woo Min, Yoo Ju Soon, Jeong Woo Jang, Gi-Hyun Lee, Sun Tae Kim*

Department of Plant Bioscience, Pusan National University

Rice blast disease, caused by Magnaporthe oryzae, is one of the major threats to rice productivity. We recently identified a M. oryzae secreted protein, MSP1, that elicit defence responses in rice. In order to analyse the global changes of MSP1 induced signalling in rice, we employed a TMT-based quantitative proteomics approach to identify the changes in cytosolic and plasma membrane proteome. This approach led to the identification of 6820 proteins of which 3832 were from plasma membrane while 2988 were from cytosolic fraction. Of these, several proteins related to the defence, proteolysis, ethylene and jasmonic acid signaling, ascorbate-glutathione metabolism and RNA regulation were upregulated. Transient expression of the MSP1 with or without signal peptide in Nicotiana bethamiana leaves showed that MSP1 is secreted out in the extracellular space which was further confirmed by the Brefeldin A (BFA) treatment. Moreover, co-infiltration of MSP1 with several phytohormones including salicylic acid (SA), abscisic acid (ABA) and methyl jasmonate (MeJA) increased ROS production, indicating a cross-talk between MSP1 induced signalling and these phytohormones signalling pathways. Interactome of the identified receptors, receptor like kinases and MAP kinases together with expression profiling showed a central role of MAPK3/6 in MSP1 induced signalling. Based on the obtained data, we propose a hypothetical model explaining MSP1 induced signalling network in rice.

PBM-81

Biological synthesis of genistein in Escherichia coli

Jeong-Hyeon Lim, Hye-Lyeong Noh, Bong-Gyu Kim*

Department of Forest Resources, Gyeongnam National University of Science and Technology

Genistein is a compound belonging to isoflavonoid found predominantly in leguminous plants. Genistein has diverse biological activities such as anthelmintic and antioxidant as well as inhibitory effects on cancer of the prostate, brain, cervix, breast and colon. Especially, genistein is well known as a phytoestrogen that shows biological effects via the estrogen receptor. In this study, we tried to synthesis biologically genistein from either p-coumaric acid or naringenin using Escherichia coli as a host of biotransformation. Four genes, Os4CL, PeCHS, RcIFS, OsCPR were used for genistein production. In order to functionally express RcIFS and OsCPR, which are a member of P450 family, in E. coli, membrane binding anchor domain of each genes was removed and then translationally fused RcIFS and OsCPR to generate RcIFS-OsCPR. Os4CL and PeCHS or RcIFS-OsCPR were transformed in BL21(DE3), respectively. Using these strains, we optimized the culture system at flask level by adjusting the cell density, different concentrations of substrate and ITPG, culture temperature and medium. Using the optimized culture condition, genistein was produced up to 35 mg/L and 13 mg/L by naringenin and p-coumaric acid supply, respectively.

PBM-82

Functional Characterization of PAMP-responsive long noncoding RNAs in Arabidopsis

Jimin Lee1, Nuri Oh2, Daeheon Jang1, Choonkyun Jung1,2*1Graduate School of International Agricultural Technology, Seoul National University, Pyeongchang, Republic of Korea, 2Crop Biotechnology Institute, Institutes of Green Bio Science and Technology, Seoul National University, Pyeongchang, Republic of Korea

Recently, long noncoding RNAs (lncRNAs) have emerged as important regulatory factors of diverse biological processes in both plants and animals. However, plant lncRNAs involved in innate immunity remain largely unknown. Plant innate immune responses are initiated upon the perception of PAMP (pathogen-associated molecular pattern) such as flagellin (flg22) and EF-Tu (elf18). In this study, we analyzed custom lncRNA array datasets generated from PAMP treatments. Overall, we identified 1,370 Arabidopsis lncRNAs induced or repressed by PAMP. Real-time RT-PCR validation confirmed the differential expression patterns of these lncRNAs in response to flg22 and elf18. Out of them, we chose 13 lncRNAs (ELENAs: elf18-induced long noncoding RNAs) that are induced by PAMP for further analysis. These lncRNAs include 10 lincRNAs (long intergenic noncoding RNAs) and 3 NATs (natural antisense transcripts). To determine whether these ELENAs regulate PTI (PAMP-triggered immunity) signaling, we examined the altered expression of innate immune response genes by overexpressing ELENAs using Arabidopsis mesophyll protoplasts. Further, we will genetically characterize the ELENA functions using gain- and loss-of-function mutants. Finally, we suggest that PAMP-responsive lncRNAs have great potential for breeding disease-resistant crops.

PBM-83

Fluorescent Adenovirus-Assisted STreamlined-Homology Directed Repair system for improved gene editing in cell lines

Hyeongseok Kim, Jisun Lee, Seungah Nam, Heungsop Shin*

Department of Chemical Engineering and Biotechnology, Korea Polytechnic University

CRISPR-Cas9 system is a powerful technique for editing target genes in almost all mammalian cell lines. In many cases, however, its practicality has been limited by the low delivery efficiency of CRISPR materials to cells. In our previous study, we developed an all-in-one adenoviral vector system to deliver Cas9 and gRNA altogether, achieving highly-efficient gene knockouts. By taking advantages of our adenoviral system, we have been able to develop a new effective gene editing method based on homology-directed repair mechanism, termed FAST-HDR (Fluorescent Adenovirus‐Assisted STreamlined‐Homology Directed Repair system). In this system, we generated novel adenoviral vectors composed of HDR template and GFP marker with Cre-loxP or PiggyBac sequences to induce a high rate of HDR-based knock-in GFP in downstream of a circadian clock gene PER1. Here we show that our new system has significantly improved the HDR knock-in efficiency to 13.2% in human cell lines.

Poster Presentation


Poster Presentation

PBM-87

SMILE regulates iron metabolism through inhibition of BMP6-mediated hepcidin expression

Yu-Ji Kim, Bo-Eun Kim, In-Young Kim, Don-Kyu Kim*


Small heterodimer partner-interacting leucine zipper protein (SMILE) belongs to basic region/leucine zipper motif transcription factors family and acts as a co-repressor of nuclear receptors. However, the role of SMILE in the iron metabolism remains unknown. Here, we demonstrate that SMILE decreases hepcidin (encoded by Hamp) expression by inhibiting BMP6-induced SMAD1 activation. Transient transfection assay indicated that BMP6-induced hepcidin promoter activity was inhibited by co-transfection of SMILE. In addition, Hepcidin promoter activity induced by co-transfection of Hfe2 (encoding Hemojuvelin (HJV)) or constitutively active form of ALK3 was decreased by co-transfection of SMILE. Adenoviral overexpression of SMILE was reduced BMP6-mediated hepcidin mRNA levels. Furthermore, co-IP and ChIP assay showed that SMILE physically interacted with SMAD1 and inhibited its binding activity to hepcidin, suggesting that SMILE suppresses BMP6-mediated SMAD1 activation leading to induction of hepcidin gene expression.Taken together, our findings reveal a previously unrecognized role of SMILE in regulation of BMP6-mediated hepcidin expression.

PBM-88

Melatonin regulates hepcidin via JNK signaling pathway

Woo-Ram Park, Byung-Yoon Choi, Don-Kyu Kim*


Hepcidin, a key hormone synthesized in the liver, regulates iron metabolism. Melatonin synthesized in the pineal gland, plays an important role in physiological actions such as circadian rhythm. Here we show that melatonin positively regulates hepcidin gene expression through activation of JNK-c-Jun in the liver. Serum iron levels change in response to circadian rhythm of hepcidin gene expression in mice. Transient transfection assay indicated that melatonin treatment increased hepcidin promoter activity. Q-PCR analysis showed that melatonin treatment increased hepcidin mRNA expression. Knockdown of melatonin receptor 1 (MT1) in AML12 cells inhibit hepcidin mRNA expression which induced by melatonin. Melatonin induced the phosphorylation of JNK and c-jun is confirmed by using western blot. Furthermore, mutation analysis and chip assay showed that c-jun directly regulates the hepcidin gene expression via AP1 of the hepcidin promoter. These results suggest that hepcidin expression show circadian rhythm that is regulated by melatonin via MT1-JNK-AP1 downstream pathway. Taken together, these findings reveal a previously unrecognized role for melatonin in the control of iron homeostasis.

PBM-85

Yield optimization with sp mutant alleles under determinate tomato

Yong Jun Kim1, Min Sung Kang1, Jung Heo1, Suk Gui Oh2, Jong Hyang Bae2, Soon Ju Park1*

1Department of Biological Science and Institute for Basic Science, Wonkwang University 460 Iksandae-ro, Iksan, Jeonbuk, Korea, 2Department of Horticulture Industry,

Wonkwang University, Iksan 54538, Korea

Controlling life span of tomato shoots is the most crucial factors for yield improvement in tomato. self pruning (sp classic) mutant inducing shoot termination has been used for breeding field tomatoes for about 100 years. Most recent question addressed that shoot life variation under determinate growth give a new window for manipulating tomato yield in the field. In this study, we isolated new three sp mutant alleles from 242 Core Collection (C.C) lines, which show variations in shoot determinacy. One deleterious sp mutants, sp-2798, resulted in no expression of sp represented similar shoot termination with less yield than sp classic. A new single amino acid substitution mutant, sp-5732, produced more sympodial shoots on main shoot and axillary shoots and improved tomato fruit yield up to 42%. The expression of transcription factors related with developmental process indicates sp-5732 is weak mutant allele of SP molecularly. Therefore, we suggest that newly discovered sp alleles are new resources for manipulating shoot growth and yield of determinate tomato in the field.

PBM-86

Bioconversion of resveratrol to δ-viniferin by grapevine peroxidases

Sung-Chul Park, Jang Won Pyun, Yu Jeong Jeong, Cha Young Kim, Jae Cheol Jeong*

Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology

The ability of grapevine to activate defense mechanisms against some pathogens has been shown to be linked to the synthesis of stilbenes, such as resveratrol and viniferins, by the plant. δ-Viniferin and ε-viniferin are stilbenoid compounds and resveratrol dehydrodimers. In a previous study, viniferin synthesis is initiated by human cyclooxygenase-1 and horse radish peroxidase via resveratrol dimerization. However, the major biosynthetic genes involved in ε-viniferin and δ-viniferin biosynthesis have not been identified in grapevine. In a previous study, we found that co-treatment of grapevine suspension cells with methyl jasmonate (MJ) and solubilizing agent (X) resulted in enhanced production of δ-viniferin in culture media. To identify resveratrol-dimerizing genes in grapevine, using transcriptome analysis, we isolated 7 putative grapevine peroxidase genes (VvAPX6, VvGPX5, VvPrx13, -21, -35, -40, and -50) that were specifically upregulated by MJ and X treatment. Overexpression of VvPrx21, VvPrx35, and VvPrx40 resulted in strong guaiacol peroxidase activities. Furthermore, HPLC analysis results revealed that crude protein extracts from transgenic Arabidopsis leaves overexpressing VvPrx21 and -35 catalyzed resveratrol dimerization to δ-viniferin in-vitro. Taken together, these results suggested that VvPrx21 and VvPrx35 might be involved in resveratrol dimerization in grapevine cells.


194

PBM-92

Divaricatic acid of Evernia mesomorpha as an antimicrobial agent for methicillin-resistant Staphylococcus aureus

Hoon Kim1*, Jong Min Oh1, Yi Jeong Kim2, Hyo-Seung Gang2, Hyong-Ho Ha1

1Department of Pharmacy, and Research Institute of Life Pharmaceutical Sciences, Sunchon National University, Suncheon 57922, Republic of Korea, 2Department of

Agricultural Chemistry, Sunchon National University, Suncheon 57922, Republic of Korea

Antimicrobial activities were evaluated against Staphylococcus aureus and Enterococcus faecium from 177 acetone s of lichen and 258 ethyl acetate extracts of cultured lichen-forming fungi (LFF) using a disk diffusion method. Evernia mesomorpha extract showed antimicrobial activity and divaricatic acid was isolated and identified by LC-MS, 1H-, 13C- and DEPT NMR. Divaricatic acid was antimicrobial activities against Gram+bacteria, i.e., Bacillus subtilis, Staphylococcus epidermidis, Streptococcus mutans, and Enterococcus faecium (MIC values ranging from 7.0 to 64.0 μg/mL), comparable to MICs of vancomycin (from 0.78 to 25.0 μg/mL). Furthermore, divaricatic acid showed higher activities than vancomycin against S. epidermidis and E. faecium. In addition, divaricatic acid was active against Candida albicans, and active as vancomycin against S. aureus (3A048; an MRSA). These results suggested that divaricatic can be used a novel antimicrobial agent for MRSA.

PBM-89

Characterization of PagAux/IAA1 gene in Poplar (Populus alba x P. glandulosa)

Jiwon Choi, Eun-Kyung Bae*

Forest Biotechnology Division, National Insitute of Forest Science

Auxin is a key player in plant growth involving phototropism, root development, stem elongation, fruit ripening and embryo formation. Especially, Auxin/Indole-3-Acetic acid (Aux/IAA) proteins are known as transcriptional repressors in plant auxin signaling by interfering auxin response factors (ARFs). However, functions of the Aux/IAA genes were understood much less in woody plants. Therefore, in this study, cDNA coding for Aux/IAA (PagAux/IAA1) was isolated from poplar (Popupus alba×P. glandulosa), and its structure and expression characteristics were investigated. PagAux/IAA1 encodes a putative protein composed of 200 amino acids, and the protein contains four conserved domains (Domain I, Domain II, Domain III, Domain IV) with bipartite nuclear localization sequence (NLS). PagAux/IAA1 gene was highly expressed in Leaves and the period from 4 days to 12 days during suspension culture. In stress conditions, mannitol and NaCl induced the lowered expression of PagAux/IAA1 gene, while cold stress induced the increased expression of the gene. On the other hand, expression of PagAux/IAA1 gene was not changed in ABA treatment, which is stress hormone. In other plant hormone treatment, expression level of PagAux/IAA1 gene was lowered by Salic acid and its expression level was increased under Gibberellin and Jasmonate. Therefore, we suggested that PagAux/IAA1 contributed to response against stress environment as well as plant development process.

PBM-90

The Phenotype Data Collection of Rice Insertion Mutants using Retrotransposon

Kyong Mi Jun1, Joung Sug Kim2, Songhwa Chae2, Gang-Seob Lee3, Baek Hie Nahm1,2*

1Plant Molecular Genetics Institute, GreenGene Biotech Inc., 2Department of Biosciences and Bioinformatics, Myongji University, 3National Academy of Agricultural Science,

Rural Development Administration

Rice is a model plant for genomic studies of monocots. With the release of complete genome sequences, the next challenge is to develop various resources based on functional analyses of genes. In this study, we generated mutants via the insertion of Tos17, a mobile endogenous retrotransposon active during tissue culture. Two rice cultivars, Oryza sativa L. japonica ‘Ilmibyeo’ and ‘Baegjinju1ho’, which represent white and brown rice in the Korean domestic market, respectively, were selected for this study. We analyzed 7,608 flanking sequences of newly transposed Tos17 insertions by the flanking adaptor-ligation polymerase chain reaction method and identified 1,672 and 843 mutants (M2 generation) in IM and BJJ1, respectively. We found new insertional mutants in 830 genes among the 1,533 genes representing 2,515 ‘Ilmibyeo’ and ‘Baegjinju1ho’ mutants that did not overlap with the 3,280 genes affected in the ‘Nipponbare’ mutants from the National Institute of Agrobiological Sciences database. Of the 1,000 lines of Tos17 insertion mutants, we observed semi-dwarf and various leaf-type mutants, including those with narrow, pale-green, and striped leaves at the vegetative stage. At the reproductive stage, 10 lines showed a 17– 56% increase in 100-grain weight compared with the wild type. This study demonstrates the potential utility of Tos17 mutants via an efficient tissue culture method in various rice cultivars for improving agronomic traits, including seed weight.

PBM-91

Sargachromanol I and G from Sargassum siliquastrum as potent acetylcholinesterase inhibitors

Hoon Kim1*, Jongmin Oh1, Myung-Gyun Kang2, Jae Pil Lee1, Joon Yeop Lee3, Seung Cheol Baek1, Hyun Hee Leem3, Daeui Park2, Myoung-Lae Cho3

1Department of Pharmacy, and Research Institute of Life Pharmaceutical Sciences, Sunchon National University, Suncheon 57922, Republic of Korea, 2Department of

Predictive Toxicology, Korea Institute of Toxicology, Daejeon 34114, Republic of Korea, 3National Development Institute of Korean Medicine, Gyeongsan 38540, Republic of Korea

Among 640 natural compounds, sargachromanol I (SCI) and G (SCG) isolated from the brown alga Sargassum siliquastrum, potently and effectively inhibited acetylcholinesterase (AChE) with IC50 values of 0.79 and 1.81 µM, respectively. Dihydroberberine (DB) isolated from Coptis chinensis, and macelignan (ML) isolated from Myristica fragrans, also potently and effectively inhibited AChE (IC50=1.18 and 4.16 µM, respectively). SCI, DB, and ML were reversible inhibitor for AChE and showed different inhibition types, i.e., mixed, competitive, and noncompetitive inhibition, respectively, (Ki =0.63, 0.77, and 4.46 µM, respectively). ML also effectively inhibited monoamine oxidase-B (IC50 =7.42 µM), suggesting it has a dual-targeting function. From docking simulations, it was obtained that the binding energy of SCI (-8.6 kcal/mol) to AChE was greater than those of SCG (-7.9 kcal/mol) and DB (-8.2 kcal/mol). It may be concluded that those four compounds, SCI, SCG, DB, and ML, can be viewed as novel reversible AChE inhibitors and promising compounds for the treatment of Alzheimer’s disease.

Poster Presentation


Poster Presentation

PBM-95

Interaction of oxidized p-phenylenediamine with lysozyme by spectroscopic methods

Semi Kim, Sohyun Kim, Moonsung Choi*


p-phenylenediamine (PPD), a derivative of p-nitroaniline, has been used for dyeing furs, photochemical measurements, accelerating vulcanization and azo-dye manufacturing, as well as for oxidizing hair dyes. It has potential toxicity which cause systemic diseases such as skin irritation and contact dermatitis, and especially includes severe edema of the eye and even permanent blindness. However, the effect of PPD to proteins has not been fully investigated. The aim of this study was to conduct the toxicity of PPD and its by-products in lysozyme after reacting with hydrogen peroxide (H2O2). After treating H2O2 to PPD, it was incubated with lysozyme, the fluorescence emission spectra of lysozyme were seen to change in the presence of various concentration of oxidized PPD at 298K. It was shown that the Stern-Volmer constant (KSV) was increased from 308K to 298K, indicating dynamic quenching. Fluorescence intensity of lysozyme decreased regularly on the emission wavelength, suggesting that oxidized PPD could interact and quench intrinsic fluorescence of lysozyme. Time dependent enzymatic activity of lysozyme was decreased in the presence of the oxidized PPD compared to the absence of it, so it can be inferred that oxidized PPD can be an inhibitor of lysozyme activity.

PBM-96

Inhibitory effect of cobalt oxidation induced by ROS on conformation and antibacterial activity of lysozyme

Sohyun Kim, Ji Hye Ahn, Moonsung Choi*


Cobalt is one of the heavy metals and it causes serious diseases such as asthma, pulmonary fibrosis, and hypothyroidism when it is overdosed. However, cobalt changes its oxidation state from Co (II) to Co (III) when they interact with reactive oxygen species (ROS). The aim of this study was to reveal the influence of ROS-induced oxidation of cobalt in the aspect of the conformation and antibacterial activity of lysozyme. Conformational change of lysozyme was assayed by intrinsic fluorescence quenching. Antibacterial activity was assessed through turbidimetric assay using Micrococcus lysodeikticus suspension by observing A450 and also compared kinetic values between Co (II) and Co (III). Significant quenching of intrinsic fluorescence of lysozyme was observed in the presence of cobalt. Co (III) showed a greater decrease of relative fluorescence units (RFU) of lysozyme. Activity assay showed that cobalt inhibited lysozyme activity, especially Co (III) inhibited lysozyme activity relatively higher. These results suggest that cobalt would affect the conformation of lysozyme especially Trp, Tyr, Phe residues and inhibit the lytic activity of lysozyme. Furthermore, ROS-induced oxidation of cobalt would affect lysozyme much severer.

PBM-93

Characterization and application of Micro-Tom Suppressor of Dwarf Mutant

Sujeevan Rajendran1*, Jung Heo1, Ryza Aditya Priatama2, Sukgui Oh3, Jong Hyang Bae3, Soon Ju Park1*

1Division of Biological Sciences and Institute for Basic Science, Wonkwang University, Iksan 54538, Republic of Korea, 2Division of Applied Life Science, Plant Molecular Biology &

Biotechnology Research Center (PMBBRC), Gyeongsang National University, Jinju 52828, Republic of Korea, 3Department of Horticulture Industry, Wonkwang University, Iksan

54538, Republic of Korea

Plant architecture affects the crop performance along with inflorescence number and fruit number. Nowadays, plant breeders are focusing on indoor farming to answer the climate change and reduced land for agriculture. Introduction of Semi-Dwarf and Dwarf varieties in major crops increased the harvest index and thus increasing the productivity of miniaturized crops. In our previous studies, we observed that the flowering time can be optimized to create a balance between vegetative and reproductive phases of a crop. Anyhow, increasing the harvest index without changing the flowering time was our goal. We have characterized a Micro-Tom mutant TOMJPG 2835-1, a mutant with increased biomass and harvest index without any changes in flowering time. We quantified major yield related traits such as inflorescence number, fruit size, fruit number, thus total yield and some architectural traits such as plant height, Plant weight, axillary branch number and length and leaf size. We observed that the inflorescence number and fruit number were greatly increased in the mutant thus increasing the yield. We located the mutations in chromosomes 1 and 2 using RNA seq analysis. Collectively 21 genes are deleted in the genome. We selected few major candidate genes and we are performing CRISPR/Cas9 based knockout for confirmation. We also made Double hybrids with Flowering time variants to synergistically increase the crop yield.

PBM-94

ASYMMETRIC LEAVES 1 induces the shading avoidance response

Young Hun Song*, Dae-Yeon Hwang

Department of Life Sciences, Ajou University

Plants have evolved an ability to adjust their morphology to the challenge imposed by a wide range of fluctuating environments. Competition for light among nearby plants induces shade avoidance responses including leaf hyponasty and changes in leaf shape. These developmental adaptations are closely related to changes in light quality, especially the red to far-red ratio, and auxin levels. Despite recent progresses in understanding shade avoidance responses, molecular mechanisms that mediate light and auxin signaling in adult plants are not well known. Here we present that phloem-specific overexpression of ASYMMETRIC LEAVES 1 (AS1) in Arabidopsis causes leaf hyponasty. The AS1 overexpressor shows increased leaf elevation angles throughout the day compared to wild type plants. The stability of AS1 protein is post-translationally regulated by light quality. Red light reduces AS1 stability, but far-red and blue light promote it. In addition, the difference in elevation angle between the AS1 overexpressor and wild type plants is relatively small under far-red and blue light conditions, indicating that changes in AS1 stability might contribute to leaf hyponasty. Consistent with the previous observation that the mutation in AS1 gene suppresses the leaf hyponasty induced by AUXIN RESISTANT 3 (axr3) mutant, the increased leaf elevation angle of the AS1 overexpressor is dramatically reduced by even low concentration of the polar AUXIN transporter inhibitor NPA.


196

PBM-100

Trait and Fertility Analysis of F1 Hybrid between Genetically Modified Brassica napus and B. rapa

Soo-In Sohn1*, Yoonsung Cho1, Hyeong Joong Kang1, Young Ju Oh2

1Biosafety Division, National Institute of Agricultural Sciences, 2Institute for Future Environmental Ecology, Institute for Future Environmental Ecology

A number of studies have been conducted on hybridization between transgenic Brassica napus and B. rapa or backcross of F1 hybrid to their parents. However, trait changes must be analyzed to evaluate hybrid sustainability in nature. In the present study, B. rapa and early flowering transgenic (BrAGL20) B. napus were hybridized to verify the early flowering phenomenon of F1 hybrids, and F1 hybrid traits were analyzed to predict their impact on sustainability. Early flowering transgenic Brassica napus L. ‘Youngsan’ (AACC, 2n=38) was transformed with CAMV 35S-regulated bar and BrAGL20 [26], and B. napus L. ‘Youngsan’ and B. rapa L. ssp. pekinensis ‘Jangkang’ (AA, 2n=20) seeds were obtained from the National Agrobiodiversity Center (Jeonju, Republic of Korea). Interspecific crossability was determined using transgenic B. napus as the pollen donor and B. rapa as the seed parent, by means of artificial emasculation and crossing. The size of F1 hybrid seeds was intermediate between those of B. rapa and transgenic B. napus, and ~40% of F1 pollen exhibited abnormal size and morphology. F2 hybrids could not be obtained, while BC1 progenies were obtained by hand pollination of B. rapa with F1 hybrid pollen, with an outcrossing rate of 50%. Our results suggest that introgression of transgenes from transgenic B. napus to B. rapa will be slowed down in nature.

PBM-97

A Study of thermodynamic parameters of Methylisothiazolinone and lysozyme by spectroscopy

and isothermal titration calorimetry

Eunji Oh, Jina Park, Moonsung Choi*


Isothiazolinones are heterocyclic organic chemicals containing vicinal sulfur and nitrogen atoms. It is not used itself while its derivatives are widely used as biocides. The methylisothiazolinone (MI), one of the isothiazolinone types is commonly used and causes severe contact allergy to mucous membranes such as eyes when exposed to the higher concentration. In this study, the interaction between MI and lysozyme was determined by detecting the spectroscopy. the thermodynamic properties of lysozyme bound with MI were investigated by isothermal titration calorimetry. The results showed that the values of Stern-Volmer quenching constants decreased with increasing temperature, which indicated that the quenching mechanism is initiated by compound formation. In titration experiments, the estimated value of stoichiometry(n) was 3.3±0.402, showing that each lysozyme molecule bound to MI occupied an effective size of 3.3 disaccharide subunits. The binding of MI to lysozyme was entropically driven, characterized by a highly favorable binding enthalpy (∆H) of -80.0±968 kcal/mol and a slightly favorable entropic contribution (-T∆S) of 75.1 kcal/mol. In conclusion, this study showed that MI had negative effect on the stability of lysozyme.

PBM-98

Inhibitory Effects of Sulfacetamide against the Lysozyme Enzymatic Activity

Jihye Ahn, Sujin Park, Moonsung Choi*


Sulfonamides are important antibiotics commonly used in human and veterinary medicine for the treatment of infectious diseases in respiratory and urinary tract. Sulfacetamide, a sulfonamide derivative, is also used as an antimicrobial to treat skin and urinary tract infections as a topical and oral agent. Though it is commonly used in human medicine, the interaction between a sulfacetamide and serum protein remained unclear. Lysozyme is an antimicrobial protein widely distributed in biological fluids including blood and tears. It serves its role as an antibacterial protein by hydrolyzing bacterial cell wall. In perspective of its significant practical role, investigating the influence of drugs on lysozyme has critical meaning upon interaction between drugs and biomacromolecules. In this study, we examined the effect of antimicrobial agent sulfacetamide on serum protein lysozyme by determining the enzymatic activity of lysozyme depend on absence and presence of the sulfacetamide. The rate of lysis of Micrococcus Lysodeikticus by lysozyme was measured turbidometrically by monitoring the lytic activity at 450 nm as a function of time. The enzymatic activity of lysozyme decreased in the presence of the sulfacetamide compared to the absence of it. The observed rate was also decreased as the concentrations of sulfacetamide were increased. Therefore, it can be inferred that sulfacetamide has an inhibitory effect on lysozyme enzymatic activity.

PBM-99

Analysis of bypass electron transfer pathways in Co2+-loaded 6His-tagged Ca2+-depleted MauG

Seungjin An1, Youngkook Moon2, Moonsung Choi1*, Sooim Shin2*1Department of Optometry, College of Energy and Biotechnology, Seoul National University of Science and Technology, Seoul 01811, Republic of Korea, 2Interdisciplinary Program of Bioenergy and Biomaterials Graduate School, College of Engineering, Chonnam National

University, Gwangju 61186, Republic of Korea

MauG is a c-type diheme protein that catalyzes the biosynthesis of tryptophan trptophylquinone (TTQ) which is required for electron transfer (ET) reaction. Ca2+ is an essential metal ion between two heme used for biosynthesis of TTQ and ET in MauG. In the absence of Ca2+, biosynthesis of TTQ could not occur. Previous studies have shown that Co2+-loaded 6His-tag at C-terminus of MauG can be substituted as a new cofactor of inactive heme in Ca2+-depleted MauG and enables to biosynthesize TTQ from premature TTQ via ET. This study used a MauG (PDB ID: 3L4M) with a 6His-tag added to the C-terminus built by pymol program, and Co2+ binding site was predicted on 6His by metal ion binding server. We characterized ET pathway and measured ET parameters, including the distance between donor and acceptor (r), packing density (ρ), electronic decaying factor (β), electron coupling (HAB) and maximum ET rate under activationless condition of Co2+-loaded 6His-tagged Ca2+-depleted MauG-preMADH complex with Harlem program.

Poster Presentation


Poster Presentation

PBM-101

The interaction of polystyrene nanoplastics with albumin by size and thermodynamic analysis

Jina Park, Eunji Oh, Moonsung Choi*


Plastic particles waste has been proven to be abundant in the aquatic and atmosphere environments including humans. It is known that nano size particles could access to all organs, translocate of blood-brain and placental barrier in living organisms. Furthermore, nanoplastics is capable of adsorbing to diverse elements, such as biomolecules in living organism, proteins, lipids, and metabolites in serum and cytoplasm. Albumin, the most abundant protein in plasma, serum, tears in human, which permits to maintain the plasma oncotic pressure and modulate the fluid distribution. Therefore, concerns were raising about their potential toxic effects, it is imperative to assess the effects of nanoplastics in human. This study was carried out to examine the effects of polystyrene nanoplastics in albumin. Dynamic light Scattering experiments were conducted, observed a change in the average size 4.5 nm increasing of average sizes (Z=34.7 nm) at 1 hour when Polystyrene (Z=30.2 nm) complexed with albumin (Z=8.5 nm). Isothermal titration calorimetry was used to study the binding interaction between albumin and Polystyrene, demonstrated the binding affinity(Kb) of albumin-Polystyrene 1.96×103 mol-1 dm3, change in binding enthalpy (∆H) of -80.0 kcal/mol. Also observed that the Gibbs free energy (∆G) value is -5.86 kcal/mol, negative which suggest that the binding of albumin with Polystyrene is spontaneous, indicating the Polystyrene-albumin complex was favored in the equilibrium albumin+Polystyrene albumin-Polystyrene.

PBM-102

Identification and characterization of OsWRKY transcription factor in the leaf architecture of rice (Oryza sativa L.)

Songhwa Chae1, Joung Sug Kim1, Kyong Mi Jun2, Yoonmok Pahk2, Duk-Ju Hwang3, Gang-Seob Lee3, Baek Hie Nahm1,2, Yeon-Ki Kim1*

1Division of Bioscience and Bioinformatics, Myongji University, 2Genomics Genetics Institute, GreenGene Biotech Inc., 3Department of Agricultural Biotechnology,

National Institute of Agricultural Science

WRKY transcription factors found exclusively in plants. Diverse biological functions in plant disease resistance, abiotic stress responses, senescence, development, embryogenesis, and hormone-controlled processes. WRKYs contain the highly conserved amino acid sequence WRKYGQK and the zinc-finger-like motifs Cys(2)-His(2) or Cys(2)-HisCys. Bind to the TTGAC(C/T) W-box cis-element in the promoter of their target genes. Therefore, it is important to identify the binding motifs of transcription factors to better understand the networks associated with development. Here, we used a rice promoter protein-binding microarray (RPBM) to identify the ATGTTTG binding motifs of OsWRKY. Among these putative target genes, there were contained transporters, protein kinases within their 2-kb promoter regions. OsWRKY were transiently expressed as fusion proteins with green fluorescent fusion protein in Nicotiana benthamiana, where they were observed to accumulate in the nucleus. For the identification of putative targets of OsWRKY36, Transcriptome analysis of the overexpression OsWRKY36 plants, combined with the RPBM results. This analysis reduced the putative targets to 6 genes. The transgenic plant showed a rolled leaf phenotype and reduction in grain yield. The shape of grass leaves possesses great value in both agronomy and developmental biology research. Leaf rolling is one of the import traits in rice breeding.


198

PNB-4

Plasma Treated Dimerization of (+)-Catechin with α-Glucosidase Inhibitory Effect

Jeong Hun Kim, Gyeong Han Jeong, Jehyeon Jo, Tae Hoon Kim*

Department of Food Science and Biotechnology, Daegu University, Gyeongsan 38453, Republic of Korea

(+)-Catechin is the naturally occurring flavan 3-ols found in many higher plant. In this study, non-thermal dielectric barrier discharge (DBD) plasma-induced structural modification of (+)-catechin (1) resulted in the isolation of the three stereochemically pure dimers with methylene linkages, methylenebicatechin (2), bis 8, 8’-catechinylmethane (3), and bis 6, 8’-catechinylmethyane (4). The structures of the newly generated catechin dimers were elucidated on the basis of spectroscopic methods. The new flavan 3-ols dimer, methylenebicatechin (2), exhibited significantly enhanced inhibitory effects toward α-glucosidase with an IC50 value of 21.2 ±0.5 μM when compared to the parent (+) -catechin. This result suggested that dimeric catechin linked through methylene bridge induced by plasma treatment of (+)-catechin might be beneficial for the prevention of diabetes.


PNB-1

Anti-inflammatory potential of an ethanolic extract of Sargassum serratifolium in nasal polyp-derived fibroblasts

Mijin Yim*

Department of Applied Research, National Marine Biodiversity institute of Korea

The purpose of this study was to assess the possible anti-inflammatory effects of Sargassum serratifolium (S. serratifolium) in nasal polyp-derived fibroblasts (NPDFs) and the associated mechanism of action. NPDFs were stimulated with Pseudomonas aeruginosa-LPS (PA-LPS) with ethanolic extract of S. serratifolium (SEE). The antioxidant activities were evaluated through ROS (Reactive oxygen species) in RAW 264.7 cells. In particular, the extract of SSE was showed significant inhibitory effect in a dose-dependent manner. To evaluate the anti-inflammatory effects of SSE, the extract of SSE was also significantly decreased on NO and PGE2 production from LPS-stimulated RAW264.7 cells.The production of collagen type I (Col-1) was investigated in nasal polyp (NP) tissue via immunohistochemistry and western blot analysis. NPDFs were treated with TGF-β1 (1 ng/mL) in the presence or absence of the extract of SSE (5-30 µM). The levels of α-smooth muscle actin (α-SMA), Col-1, and fibronectin were decreased by western blot analysis. In contrast, incubation with TGF-beta1 increased fibronectin, procollagen, vascular endothelial growth factor (VEGF) of the extract of SSE (5-30 µM). The VEGF protein was decreased in a dose-dependent manner. These results suggest that the extract of SSE may be a novel antifibrotic agent for the treatment of NP formation.

PNB-2

Molecular basis involved in the blocking effect of antidepressant metergoline on C-type inactivation of

Kv1.4 channel



Voltage-gated potassium channels (VGKCs) are transmembrane ion channels specific for potassium. Currently there are nine kinds of VGKCs. Kv1.4 is one of shaker-related potassium channels. It is a representative alpha subunit of potassium channels that can inactivate A type-currents, leading to N pattern inactivation. Inactivation of Kv channels plays an important role in shaping electrical signaling properties of neuronal and muscular cells. The shape of N pattern inactivation can be modified by removing the N-terminal (NT) domain which results in non-inactivated currents and C pattern inactivation. In the present study, we constructed a mutant of deleted 61 residues from NT of Kv1.4 channels (Kv1.4 D2-61) and found that it induced an outward peak and steady-state currents. Interestingly, metergoline treatment induced little effects on the outward peak current in the deleted Kv1.4 mutant channel. However, metergoline treatment conspicuously inhibited steady state currents of Kv1.4 D2-61 channels with acceleration current mode. The acceleration of steady-state current of deleted Kv1.4 mutant channel occurred in a concentration-dependent manner. This means that metergoline can accelerate C pattern inactivation of Kv1.4 D2-61 channel by acting as an open state dependent channel blocker. Taken together, these results demonstrate the molecular basis involved in the effect of metergoline, an ergot alkaloid, on human Kv1.4 channel, providing a novel interaction ligand.

PNB-3

Effects of Alisma Rhizome on ligand-gated ion channels activity



Alisma Rhizome is a well-known traditional East-Asian medicine which has been widely used for diuretic, anti-inflammatory, hypolipidaemic, and anti-diabetic for thousands of years. In present study, we investigated the effect of Alisma Rhizome on human 5-hydroxytryptamine 3A (5-HT3A) receptor and a3b4 nicotinic acetylcholine (nACh) receptor channel activity expressed in Xenopus oocytes. Co-application with Alisma Rhizome inhibited the 5-HT3A and a3b4 nACh receptor-mediated inward peak current. This inhibitory effect of Alisma Rhizome on 5-HT and ACh-induced inward current (I5-HT and IACh) occurred in a concentration dependent and reversible manner. Half maximal inhibitory concentrations (IC50) of Alisma Rhizome were 79.4±11.0 and 21.2±6.0 mg/ml in 5-HT3A and a3b4 nACh receptor, respectively. In addition, inhibition of I5-HT and IACh by Alisma Rhizome occurred in a non-competitive and voltage-insensitive manner. Taken together, these results indicate that Alisma Rhizome may regulate the expressed 5-HT3A and a3b4 nACh receptors in Xenopus oocytes.

Poster Presentation


Poster Presentation

PNB-8

Antifungal Potential of Extracts from Different Parts of Mulberry Plant



In the present study, we investigated the antifungal activity of methanol and ethanol extracts of different parts (leaves, twigs, and root bark) of mulberry plant against Alternaria alternata and Fusarium sp. Among them, the methanol and ethanol extracts of mulberry root bark exerted the highest inhibitory activity against the mycelial growth of A. alternata (70.6±1.6 to 80.8±6.7% and 58.7±0.0 to 80.8±6.7%, respectively) and Fusarium sp. (15.5±2.7 to 39.3 ±3.4% and 26.4 ±2.7 to 47.6 ±4.8%, respectively). In contrast, the methanol and ethanol extracts from mulberry leaves and twigs did not suppress the mycelial growth of these fungal species. Importantly, the methanol and ethanol extracts of mulberry leaves tended to even accelerate the mycelial growth of A. alternata and Fusarium sp. Therefore, the results of this study indicate that methanol and ethanol extracts of mulberry root bark can be used as control agents against A. alternata and Fusarium sp.

PNB-5

Studies on Chemical Constituents from Gnetum macrostachyum

Changon Seo1, Won Sik Jeong1, Jin Gwan Kwon1, Yeon Wo Jung1, Jung A Lee1, Eun-Kyung Ahn1, Jae-Shin Kang2, Woo Hee Kim2, Jin Kyu Kim1, Chun Whan Choi1,

Joa Sub Oh3, Seong Su Hong1*1Bio-Center, Gyeonggido Business & Science Accelerator (GBSA), 2Biological Genetic

Resources Utilization Division, National Institute of Biological Resources (NIBR), 3College of Pharmacy, Dankook University

The Gnetum macrostachyum was extracted with 50% EtOH and partitioned with n-hexane, CH2Cl2, EtOAc, n-BuOH and aqueous fractions. The purification of CH2Cl2 and EtOAc fractions by column chromatography separation and HPLC analysis to yield compounds 1-10. The structures of the isolated compounds were established as tyrosol (1), homovanillyl alcohol (2), vanillin (3), lehmbachol D (4), isorhapontigenin (5), (2S)-naringenin 8-C-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside (6), isohemiphloin (7), isorhaponcitin (8), gnemonol M (9), and resveratrol (10) by analysis of NMR and MS data, along with comparison with those in the literatures. Among them, compounds 2, 6-8 were identified from Gnetum genus for the first time and compounds 1, 3, 4 and 9 were isolated from G. macrostachyum for the first time.

AcknowledgementsThis study was supported by a grant from the National Institute of Biological Resources (NIBR) under the Ministry of Environment (MOE) of the Republic of Korea (NIBR No. 2019-06-203).

PNB-6

Combrequadranols A and B, two new cycloartane triterpenes from the stems of Combretum quadrangulare

Changon Seo1, Ji Eun Lee1, Yun-Hyeok Choi1, Jae-Shin Kang2, Chang Woo Hyun2, Jung A Lee1, Eun-Kyung Ahn1, Jin Gwan Kwon1, Jin Kyu Kim1, Chun Whan Choi1,

Joa Sub Oh3, Seong Su Hong1*1Bio-Center, Gyeonggido Business & Science Accelerator (GBSA), 2Biological Genetic

Resources Utilization Division, National Institute of Biological Resources (NIBR), 3College of Pharmacy, Dankook University

Two new cycloartane triterpenoids, named combrequadranols A and B (1 and 2), and 15 known metabolites (3-17) were isolated from an ethanolic extract of the stems of Combretum quadrangulare (family Combretaceae) using bioassay-guided fractionation. Their structures were elucidated by a combination of 1D and 2D NMR, and HRESI-MS. In addition, 14 previously described eleven flavonoids (3-9 and 11-14), two phenolic analogues (10 and 17), an ellagitannin (15), and a chromone C-glucoside (16) were identified when we compared their spectroscopic data to literature values, namely ayanin (3), vitexin (4), retusin (5), kamatakenin (6), 3,4’-dimethoxy-5,7,3’-trihydroxyflavone (7), 3,7-dimethoxy-5,3’,4’-trihydroxyflavone (8), (2R,3R)-7,3’-di-O-methyltaxifolin A (9), methyl vanillate (10), 4’,5-dihydroxy-3’, 7-dimethoxyflavanone (11), combretol (12), isoorientin (13), orientin (14), casuarinin (15), biflorin (16), and ellagic acid (17). To the best our knowledge, compounds 5, 9-11, and 13 were obtained from the family Combretaceae for the first time. Moreover, compounds 8, 15 and 17 has not been reported in the species of C. quadrangulare.

AcknowledgementsThis study was supported by a grant from the National Institute of Biological Resources (NIBR) under the Ministry of Environment (MOE) of the Republic of Korea (NIBR No. 2019-06-203).

PNB-7

Beneficial Effects of Safflower (Carthamus tinctorius L.) Seed on Scopolamine-Induced Memory Impairment in Mice

Chan Hum Park1, Min Jo Kim1, Chang Yeol Yang1, Ji Hyun Kim2, Meitong He2, Eun Ju Cho2, Takako Yokozawa3, Yu Su Shin1*

1Department of Medicinal Crop Research, NIHHS, RDA, Eumseong 27709, Republic of Korea, 2Department of Food Science and Nutrition & Kimchi Research Institute, Pusan National University, Busan 46241, Republic of Korea, 3Graduate School of Science and

Engineering for Research, University of Toyama, Toyama 930-8555, Japan

Alzheimer’s disease (AD) is the most common type of aging-related neurodegenerative disorder. Cholinergic dysfunction and oxidative stress have been proposed to play a vital role in the onset and progression of AD. Safflower seed (Carthamus tinctorius L.)-derived serotonin and its derivatives which have antioxidant and anticholinergic activities. This study examined whether safflower seed has memory-enhancing effect in scopolamine-induced memory impairment mice model. Safflower seed water extract (SWE) was orally administered at dose of 100 mg/kg/day, and then behavior tests such as T-maze and novel object recognition test were conducted. Acetylcholinesterase (AChE) activity, reactive oxygen species (ROS) production, and antioxidant enzymes in the brain were measured. In behavior tests, novel route and object recognitions were improved by administration of SWE, indicating that SWE improved memory function in the scopolamine-treated mice. Also, SWE-administered group showed inhibition of the AChE activity. In addition, administration of SWE showed the lower ROS production and higher antioxidant enzymes levels compared with the scopolamine-treated vehicle group. The present results suggest that SWE improves scopolamine-induced memory deficits via inhibition of cholinergic dysfunction and oxidative stress. As a result of HPLC analysis, SWE contained serotonin and its derivatives such as N-(p-coumaroyl) serotonin and N-(feruloyl) serotonin. Therefore, safflower seed might become a natural therapeutic agent for protecting against memory impairment.


200

PNB-12

Geranialdehyde and Its Structural Derivatives Show Acaricidal Properties against Dermatophagoides spp.

and Haemaphysalis longicornis

Ah-Hyeon Jeong1, Jun-Hwan Park1, Hui-Ju Kim1, Hoi-Seon Lee1*, Jeong-Moon Kim2*

1Department of Bioenvironmental Chemistry, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju 54896, Korea, 2Department of Landscape Architecture, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju 54896, Korea

Severe fever with thrombocytopenia syndrome caused by severe fever with thrombocytopenia syndrome virus is an emerging infectious disease with 15-30% fatality rate. Despite its severity, no treatment for severe fever with thrombocytopenia syndrome has been developed. The only method of preventing the spread of severe fever with thrombocytopenia syndrome is to control the arthropod-borne vector of the SFTS virus. Geranialdehyde (3,7-dimethylocta-2,6-dienal) analogues derived from Melissa officinalis leaves have shown an acaricidal function against Haemaphysalis longicornis, known to harbor the severe fever with thrombocytopenia syndrome virus, and the house dust mite, Dermatophagoides spp., and an indicative function (color change) on Dermatophagoides spp. In a fumigant bioassay, 2,4-octadienal (LD50, 0.93, 0.86, and 0.86 μg/cm3) was 29.4-, 29.5-, and 58.2-fold more toxic than DEET (LD50, 27.23, 25.47, and 49.72 μg/cm3) against D. farinae, D. pteronyssinus, and H. longicornis, respectively, followed by 3,7-dimethyl-6-octenal (LD50, 1.21, 1.42, and 0.49 μg/cm3), 2-octenal (2.84, 2.02, and 0.25 μg/cm3), and M. officinalis oil (2.06, 1.42, and 1.97 µg/cm3). Moreover, the color of D. farinae and D. pteronyssinus was altered from colorless to golden brown by treatment with M. officinalis oil, geranialdehyde, 3,7-dimethyl-6-octenal, and 2,4-octadienal. The results suggested that M. officinalis oil, geranialdehyde, 3,7-dimethyl-6-octenal, and 2,4-octadienal could be developed as functional targeting agent for severe fever with thrombocytopenia syndrome virus.

PNB-9

Acaricidal Properties of (1S,4R,5R)-4-Methyl-1-(propan-2-yl)bicyclo[3.1.0]hexan-3-one and Its Structural Derivatives

against Mites

Hui-Ju Kim1, Seon-A Choi1, Jeong-Bin Lee2, Min-Seung Kang1, Hoi-Seon Lee1*1Department of Bioenvironmental Chemistry, College of Agriculture and Life Sciences,

Chonbuk National University, Jeonju 54896, Korea, 2Department of Landscape Architecture, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju 54896, Korea

Acaricidal activities of Salvia officinalis seed oil against Dermatophagoides farinae, D. pteronyssinus and Tyrophagus putrescentiae were evaluated using fumigant bioassay. Based on the LD50 values against D. farinae, D. pteronyssinus, and T. putrescentiae, the essential oil (LD50, 2.53 μg/cm3, 2.74 μg/cm3 and 3.70 μg/cm3) of S. officinalis seeds was about 3.86, 3.69, and 2.94 times more active higher than benzyl benzoate (9.79 μg/cm3, 10.13 μg/cm3

and, 10.90 μg/cm3). To explore the acaricidal activities of the essential oil against Dermatophagoides spp. and T. putrescentiae, the chemical composition of the essential oil was analyzed by GC-MS. The essential oil of S. officinalis consisted mainly of (-)-α-(1S,4R,5R)-4-methyl-1-(propan-2-yl)bicyclo[3.1.0]hexan-3-one (33.62%). Based on the LD50 values of the constituents derived from S. officinalis seed oil, (-)-α-(1S,4R,5R)-4-methyl-1-(propan-2-yl)bicyclo[3.1.0]hexan-3-one (LD50, 2.80, 2.92 and 3.94 μg/cm3) showed strong acaricidal properties against D. farinae, D. pteronyssinus, and T. putrescentiae, using fumigant bioassay. The acaricidal properties of S. officinalis oil could be attributed to (-)-α-thujone. Based on the structure-toxicity relationship between (-)-α-(-)-α-(1S,4R,5R)-4-methyl-1-(propan-2-yl)bicyclo[3.1.0]hexan-3-one analogues and the acaricidal activities against D. farinae, D. pteronyssinus, and T. putrescentiae, the LD50 values demonstrated that α, β-(-)-α-(1S,4R,5R)-4-methyl-1-(propan-2-yl)bicyclo[3.1.0]hexan-3-one (3.17, 3.43 and 4.26 μg/cm3) showed acaricidal activity against D. farinae, D. pteronyssinus, and T. putrescentiae. (-)-α-(1S,4R,5R)-4-Methyl-1-(propan-2-yl)bicyclo[3.1.0]hexan-3-one analogues have promising potential as natural acaricides against D. farinae, D. pteronyssinus, and T. putrescentiae.

PNB-10

Biological Properties of 2,2,4,4-Tetramethyl-6-(3-methylbutanoyl) cyclohexane-1,3,5-trione and Structure-Activity Relationships

of Its Derivatives

Hui-Ju Kim, Ah-Hyeon Jeong, Seon-A Choi, Hoi-Seon Lee*


Biological properties of 2,2,4,4-tetramethyl-6-(3-methylbutanoyl)cyclohexane-1,3,5-trione (leptospermone) isolated from the Leptospermum scoparium oil and its structural derivatives (1,3-cyclohexanedione, 1,2,3-cyclohexanetrione-1,3-dioxime, 5,5-dimethyl-1,3-cyclohexanedione and 2,2,4,4,6,6-hexamethyl-1,3,5-cyclohexanetrione) were evaluated by agar diffusion method against foodborne bacteria, Listeria monocytogenes, Staphylococcus aureus, S. intermedius, Salmonella typhimurium, Shigella flexneri and S. sonnei. The essential oil of L. scoparium possessed potent antimicrobial activity against the six bacteria tested. The antimicrobial compound of L. scoparium was isolated by various chromatographic analyses and identified as 2,2,4,4-tetramethyl-6-(3-methylbutanoyl)cyclohexane-1,3,5-trione. To investigate the structure-activity relationships, the antimicrobial activities of 2,2,4,4-tetramethyl-6-(3-methylbutanoyl)cyclohexane-1,3,5-trione and its structural derivatives were examined against six foodborne bacteria. Based on the MIC values, 2,2,4,4-tetramethyl-6-(3-methylbutanoyl)cyclohexane-1,3,5-trione (MIC 23.6-69.7 μg/mL), 1,2,3-cyclohexanetrione-1,3-dioxime (MIC 43.9-88.5 μg/mL) and 2,2,4,4,6,6-hexamethyl-1,3,5-cyclohexanetrione (MIC 48.1-75.8 μg/mL) exhibited antimicrobial activities against six foodborne bacteria. These results indicated that 2,2,4,4-tetramethyl-6-(3-methylbutanoyl)cyclohexane-1,3,5-trione and its structural derivatives give a chance for developing natural food preservatives to rather hazardous synthetic preservatives.

PNB-11

Insecticidal Properties of 1,4-Naphthalenedione Isolated from Cercis chinensis Heartwood and Its Structurally

Related Derivatives

Ah-Hyeon Jeong, Hui-Ju Kim, Hoi-Seon Lee*


Our study was to determine the larvicidal activity of an active compound isolated from Cercis chinensis heartwood and its structurally related derivatives (5-hydroxy-1,4-naphthalenedione, 2-bromo-1,4-naphthalenedione, 2-hydroxy-1,4-naphthalenedione, 2-methoxy-1,4-naphthalenedione, and 2-methyl-1,4-naphthalenedione) against 4th-stage Aedes aegypti, Culex pipiens pallens, and Ae. togoi. The larvicidal compound of C. chinensis was isolated with the use of various chromatographic techniques and identified as the derivatives of 1,4-naphthalenedione. Based on the median lethal concentration (LC50) values of commercially procured derivatives against Ae. aegypti larvae, the most toxic derivative was 2-bromo-1,4-naphthalenedione (1.19 μg/ml); followed by 5-hydroxy-1,4-naphthalenedione (1.72 μg/ml); 2-methyl-1,4-naphthalenedione (9.12 μg/ml); 2-hydroxy-1,4-naphthalenedione (10.76 μg/ml); and 2-methoxy-1,4-naphthalenedione (12.50 μg/ml). Similar results were observed against Cx. p. pallens and Ae. togoi larvae with 1,4-naphthalenedione derivatives. These results also show that 1,4-naphthalenedione structural derivatives were less toxic than the organophosphate pirimiphos-methyl. Nonetheless, naturally occurring C. chinensis-derived materials and 1,4-naphthalenedione structural derivatives have potential for development as mosquito larvicidal agents.

Poster Presentation


Poster Presentation

PNB-16

Antioxidant phytochemicals from Ikonnikovia kaufmanniana and their protection of DNA damage

Kamila Zhumanova, Aizhamal Baiseitova, Jeong Yoon Kim, Ki Hun Park*


Ikonnikovia kaufmanniana is the endemic plant of Kazakhstan of which phytochemical analysis has not been reported. This study found out that this species had antioxidant enriched chemicals. Isolation and structural identification processes gave twelve phenolic compounds (1-12) having dihydroflavanonol, flavonol, isoflavone and flavanol skeletons. The antioxidant potential of the compounds was found to differ by their functionality and skeleton. This study selected first the phenolic compounds (1, 5, 7 and 8) which have significant potentials to hydroxyl radical that is highly connected with DNA damage. The compounds (1, 5, 7 and 8), showed a significant protective effect on pBR322 plasmid DNA against mutagenic and toxic effects of Fenton’s reactions. The most active compound 8, displayed a dose-dependent DNA damage protection potential in the range of 15~120 μM. The hydroxyl radical scavenging activity was found to have a higher correlation with DNA damage protection potential.

PNB-13

Repellent Activities of Litsea cubeba (Lour.) Fruit Oil and Its Major Components against Haemaphysalis longicornis

(Acari: Ixodidae)

Hui-Ju Kim, Ah-Hyeon Jeong, Jun-Hwan Park, Hoi-Seon Lee*


The bioactivity of the essential oil extracted from Litsea cubeba fruits on severe fever with thrombocytopenia syndrome virus vector has never previously been reported. In this study, L. cubeba oil and its main constituent, 3,7-dimethyl-2,6-octadienal and structural derivatives, were evaluated for their repellent activities on the unfed nymphs and adults of Haemaphysalis longicornis. In the repellent tests, L. cubeba oil showed repellent activities against the unfed nymphs and adults of H. longicornis. Among the L. cubeba oil’s constituents, only 3,7-dimethyl-2,6-octadienal exhibited repellent activities against the unfed nymphs and adults. Eucalyptol, (+)-limonene, a-pinene and b-pinene showed no repellent activity against the unfed nymphs and adults. In repellent bioassay using a choice device equipped with silicone membrane feeding system, 3,7-dimethyl-2,6-octadienal and L. cubeba oil at dose 0.08 mg/cm2 to the unfed nymphs and adults gave excellent repellence with 100% for at least 60 min post-application. With respect to the comparative study of the repellent activity of 3,7-dimehtyl-2,6-octadienal and its structural derivatives, all tested derivatives were significantly less potent than those of 3,7-dimehtyl-2,6-octadienal. These results indicate the strong influence of carbon– carbon double bonds and functional group (methyl group) on the repellent activities of compounds. The strong repellent activities of 3,7-dimethyl-2,6-octadienal and L. cubeba oil may suggest them as promising natural candidates for new sustainable repellent agents.

PNB-14

Acaricidal Properties of Alpinia galanga (L.) Rhizome Oil and Its Major Components against Haemaphysalis longicornis

(Acari: Ixodidae)

Hui-Ju Kim1, Min-Seung Kang1, Ah-Hyeon Jeong1, Jeong-Monn Kim2, Hoi-Seon Lee1*

1Department of Bioenvironmental Chemistry, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju 54896, Korea, 2Department of Landscape Architecture, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju 54896, Korea

The control of Haemaphysalis longicorn is as 3-host life cycler has been a much bigger concern because it has humans and animals as host and infectious disease of severe fever with thrombocytopenia syndrome. Despite its severity, no treatment for severe fever with thrombocytopenia syndrome has been developed. In this study, the essential oils of Alpinia galanga rhizome cultivated in India, Indonesia and Thailand and its main components, ethyl cinnamate, ethyl methoxycinnamate, methyl cinnamate and derivatives, were evaluated for their acaricidal activities against the unfed larvaes and nymphs of H. longicornis. Using the packet test against the unfed larvaes and nymphs, the essential oils of A. galanga rhizome cultivated in India and Indonesia showed acaricidal activities against unfed larvaes and nymphs. Among the essential oil’s components of A. galanga rhizome cultivated in India and Indonesia, only ethyl cinnamate, ethyl methoxycinnamate, and methyl cinnamate exhibited acaricidal activities against unfed larvaes and nymphs. Based on the LC50 values against unfed larvaes, benzyl cinnamate (LC50, 1.41 mg/mL) had the most effective activities than amitraz (LC50, 2.41 mg/mL). Based on the LC50 values against unfed nymphs, allyl cinnamate (LC50, 6.92 mg/mL) had the most potent activities than amitraz (LC50, 26.77 mg/mL). These results indicated that the strong influence of trans-cinnamate group on the acaricidal activities of compounds against H. longicornis.

PNB-15

Dual Properties of 5-Methylfurfural Derived from Valeriana fauriei and Its Structural Derivatives against Dermatophagoides spp., Tyrophagus putrescentiae and Haemaphysalis longicornis

Ah-Hyeon Jeong, Hui-Ju Kim, Seon-A Choi, Hoi-Seon Lee*


The potential properties of 5-methylfurfural derived from the essential oil of Valeriana fauriei and its structural derivatives to act as acaricide and mite indicator against Dermatophagoides farinae, D. pteronyssinus, Haemaphysalis longicornis and Tyrophagus putrescentiae were showed in this study. The V. fauriei oil was obtained by steam distillation extraction and analyzed by GC-MS. Seventeen compounds were characterized, and the six major components were tested against Dermatophagoides spp., H. longicornis and T. putrescentiae using contact + fumigant filter paper bioassay. Based on the LD50 values, 5-methylfurfural (14.27 μg/cm2, 15.23 μg/cm2 and 8.59 μg/cm2) showed the highest acaricidal activity against Dermatophagoides spp. and T. putrescentiae. Nevertheless, the V. fauriei oil and its constituents showed no acaricidal activity against H. longicornis. To investigate the structure-activity relationships, the acaricidal activities of 5-methylfurfural and its structural derivatives (furan, 2-methylfuran, furfural, 4,5-dimethylfurfural and 5-hydroxy-2-methylfurfural) were examined. Among 5-methylfurfural structural derivatives, the most toxic compound was 4,5-dimethylfurfural (LD50; 9.95 μg/cm2, 9.91 μg/cm2 and 10.04 μg/cm2) against Dermatophagoides spp. and T. putrescentiae, respectively. Interestingly, color alteration of Dermatophagoides spp. and T. putrescentiae was exhibited, changing from colorless to red brown through the treatment with 4,5-dimethylfurfural, furfural and 5-methylfurfural. These results suggest that the V. fauriei oil and 5-methylfurfural could be developed as new effective acaricide and mite indicator for control of synanthropic mites.


202

PNB-20

Diarylheptanoid derivatives from the Zingiber officinale rhizomes and their anti-oxidant activity

Yeong-Geun Lee1, Hyoung-Geun Kim1, Youn-Hyung Lee2, Se Chan Kang1, Tong Ho Kang1, Nam-In Baek1*

1Graduate School of Biotechnology and Department of Oriental Medicine Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea, 2Department of Horticultural

Biotechnology of College of Life Sciences, Kyung Hee University, Yongin 17104, Republic of Korea

Zingiber officinale is a herbaceous perennial plant, which has narrow, bright green, grass-like leaves and yellowish green flowers with purple markings. The rhizomes of Z. officinale has been widely used in Korean traditional medicine for the treatment of gastrointestinal ailments such as motion sickness, dyspepsia and hyperemesis gravidarum. The aim of this study was the isolation and identification of diarylheptanoid derivatives from this plant, investigation of anti-oxidant effect, as well as explaining structure-activity relationship for the anti-oxidant effect.Fresh rhizomes of Z. officinale were extracted with aqueous EtOH, and the concentrated extract was partitioned into n-hexane, EtOAc, n-BuOH, and H2O fractions. As a result of repeated SiO2, ODS, and Sephadex LH-20 column chromatographies on n-hexane and n-BuOH fraction, revealed a new diarylheptanoid derivatives (14) along with 13 known ones (1-13) were isolated. From the analysis of spectroscopic data the compounds were respectively identified to be 6-,8-paradols (1,2), trans-6-,8-,10-shogaols (3-5), 6-,8-,10-gingerols (6-8), 5-methoxy-6-,10-gingerol (9, 10), dehydro-6-,8-gingerdione (11, 12), and 6-,8-gingerdiol 4’-O-β-D-glucopyranoside (13, 14) without ambuiquity.Almost diarylheptanoid derivatives and its EtOH extracts showed high radical scavenging potential in DPPH, ORAC, and ABTS test. Also quantitative analysis in the rhizomes of Z. officinale was conducted through HPLC experiment. These results supported that the diarylheptanoid derivatives isolated from the rhizomes of Z. officinale and its EtOH extract, especially n-hexane fraction, are potential materials as anti-oxidant agents.

PNB-17

New polyprenylated acylophoroglucinol from the flowers of Hypericum ascyron

Abdul Bari Shah, Yeong Jun Ban, Zuo Peng Li, Ki Hun Park*


Hypericum ascyron belongs to the Guttiferae family and is widely distributed in eastern Asia. It has been used as a traditional Chinese medicine for treatment for headache, rheumatism, and abscesses. This plant is well-known for unique and complexed metabolites based on polycyclic polyprenylated acylphloroglucinols (PPAPs). PPAPs have been applied to various biological targets, such as antidepressant, anti-inflammatory, antiviral, antibacterial, anti-tumor, anti-neurodegenerative, and anti-HIV. This study isolated new acylphoroglucinol from the flowers of Hypericum ascyron. The structure was fully characterized by HREIMS, 2D-NMR, and CD spectra. The successive NOE cross peaks between H29, H11 and H34 revealed that prenyl at C5, methyl at C6 and prenyl at C10 are in the same direction. The prenyl at C3 is on the same side with the benzoyl group at C8 because of NOE cross peak between H24 and H14. Furthermore, it is natural that methyl at C6 and prenyl at C3 must exist in the same direction, when we consider the uniqueness of tricycle [4.3.1.0] decane structure. Thus the structure of 1 was determined ascyrones E. The electronic circular dichroism (ECD) spectrum of 1 exhibited the cotton effects at 235 nm (De+7.8) and 328 nm (De+16.7) and a negative cotton effect 290 nm (De-20.4) defined absolute configuration as 1R, 3R, 5S, 6S, 7R, 10S.

PNB-18

Caged Xanthones from Garcinia hanburyi inhibit Human Neutrophil Elastase

Da Hyun Jang, Jeong Ho Kim, Jeong Yoon Kim, Ki Hun Park*


Garcinia hanbury is small to a medium-sized tree and distributed in the Southern part of Thailand. These species secrete a yellow gum resin called as gamboge by incision of stem or by breaking off the shoots. Gamboge has been widely used as a dye and folk medicine for potent purgative and infected wound. This study isolated four caged xanthones from gamboge. Their structures were identified as gambogoic acid (1), moreollic acid (2), gambogic acid (3), and 10-methoxygambogenic acid (4), respectively. All isolated compounds were applied to human neutrophil elastase (HNE) which plays a critical role in the initiation and progression of pulmonary inflammation. HNE may be activated upon exposure to various cytokines, and chemoattractants including tumor necrosis factors. All compounds showed a potent enzyme inhibition against HNE with IC50 values of 43.7~142.7 µM. Most active compound was found to be moreollic acid (IC50 =43.7 µM). In kinetic study, all compounds were proved as a mixed type inhibitor against HNE.

PNB-19

6-Metoxyflavonols from the aerial parts of Tetragonia tetragonioides (Pall.) Kuntze and their anti-inflammatory activity

Yeong-Geun Lee1, Jin Ah Ryuk2, Joo Tae Hwang2, Hyoung-Geun Kim1, Youn-Hyung Lee3, Dong Sung Lee4, Byoung-Seob Ko2, Nam-In Baek1*

1Graduate School of Biotechnology and Department of Oriental Medicine Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea, 2Korea Institute of Oriental

Medicine, Daejeon 34054, Republic of Korea, 3Department of Horticultural Biotechnology of College of Life Sciences, Kyung Hee University, Yongin 17104, Republic of Korea,

4College of Pharmacy, Chosun University, Gwangju 61452, Republic of Korea

Tetragonia tetragonoides (Aizoaceae) has been used in Korean traditional medicine for treatment of various diseases such as inflammation, ulcers, and stomach cancer. T. tetragonoides contains several secondary metabolites such as flavonoid, alkaloid, and terpene. These compounds have been reported to treat ulcers and stomach cancer. However, pharmacological studies of T. tetragonoides on anti-inflammation is barely carried out. Therefore, the present study focused on isolation of activte materials from T. tetragonoides aerial parts, structure determination of the isolated ones, the interaction between anti-oxidation and pro-inflammatory markers, and the structure-activity relationship for the anti-inflammatory effect.Dried aerial parts of T. tetragonoides were extracted with 70% EtOH, and the evaporated residue was successively separated into EtOAc, n-BuOH, and H2O fractions. As a result of repeated SiO2, ODS, and Sephadex LH-20 column chromatography, four new 6-methoxyflavonol glycosides along with four known ones were isolated and structure elucidation by various spectroscopic data such as NMR, IR, FAB/MS.Almost compounds showed high radical scavenging effect in ABTS and DPPH and anti-inflammatory activities such as reduction of NO and PGE2 formation and suppression of TNF-α, IL-6, IL-1β, iNOS, and COX-2 expression in LPS-stimulated RAW 264.7 macrophages. These results supported that the 6-methoxyflavonols isolated from the aerial parts of T. tetragonoides and its EtOH extract are potential as anti-oxidant and anti-inflammatory agents.

Poster Presentation


Poster Presentation

PNB-24

Enhanced Protein Self Assembly through Novel Water Soluble Nitrilotriacetic Acid Ligands

Hee-Kwon Kim*


Ni(II)−Nitrilotriacetic acid (NTA) showed high-affinity and specificity and to Histidine-tagged proteins, and the histidine /Ni(II)-nitrilotriacetic acid (Ni2+-NTA) system is widely used for protein purification, and fluorescence labeling. It was known that protein nucleation is an initial key procedure to influence all over protein crystallization process. Thus controlling protein nucleation is important to increase protein crystallization. In this study, symmetric and novel multivalent nitrilotriacetic acid ligands were designed to enhance protein crystallization via formation of ligand:protein complexes. These ligands were highly symmetric and water-soluble, and they are synthesized through coupling reaction of core moiety. In the treatment of ligands with green fluorescent protein (GFP), larger complexes of GFP protein:ligand was formed via intermolecular interactions, which allowed protein nucleation to be enhanced. Our results suggested that novel multivalent nitrilotriacetic acid chelating agents can increase protein nucleation and self-assembly step.

PNB-21

New phenolics and triterpenoids from Lithocarpus mekongensis and their antioxidant activity and protective

effects on auditory hair cell damaged by neomycin in zebrafish (Danio rerio) larvae

Hyoung-Geun Kim1, Youn Hee Nam1, Sun-Woo Joo1, Young Sung Jung2, Yeong-Geun Lee1, Dae-Ok Kim2, Youn-Hyung Lee3, Dae Young Lee4, Nam-In Baek1*

1Graduate School of Biotechnology & Department of Oriental Medicine Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea, 2Department of Food Science and Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea, 3Graduate School

of Biotechnology & Department of Horticultural Biotechnology, Kyung Hee University, Yongin 17104, Korea, 4Department of Herbal Crop Research, National Institute of

Horticultural and Herbal Science, RDA, Eumseong 27709, Republic of Korea

Lithocarpus mekongensis (Fagaceae) is a perennial shrub distributed in Yunnan County in China, Laos, and Vietnam. This plant was first identified at 1992 by C.C. Huang and Y.T. Chang. While various chemical constituents and physiological activities have been reported for Lithocarpus species, there is no study of L. mekongensis to date. In our preliminary study, methanol extract and ethyl acetate fraction of L. mekongensis showed the significant protective effects on auditory hair cell damaged by neomycin in zebrafish (Danio rerio) larvae. Therefore, the present study focused on the isolation and identification of active materials from this plant, as well as evalutaion of radical scavenging activities using DPPH and ABTS radicals and protective effects on auditory hair cell (AH) damaged by neomycin in zebrafish larvae (NZL).The aerial parts of L. mekongensis were extracted with aqueous methanol, and the concentrared extract was partitioned into EtOAc, n-BuOH, and H2O fractions. The repeated column chromatographies for EtOAc and n-BuOH fractions led to isolation of three phenolic compounds and three triterpenoids from L. mekongensis. Based on spectroscopic data, one new lignan, lithomekongenol A (1), two known phenolics (2 and 3), and three known triterpenoids (4−6), were structurely identified from L. mekongensis. Some compounds showed radical scavenging activities in DPPH and ABTS radical experiments. Moreover, some compounds exhibited protective effects on AH damaged by NZL.

PNB-22

New isocoumarins and flavonoids from the fruits of Citrus grandis Osbeck and their antioxidant activity and protective effects on pancreatic islet damaged by alloxan in zebrafish

(Danio rerio) larvae

Hyoung-Geun Kim1, Yoon Hee Nam1, Hyun-Ji Oh1, Joo Tae Hwang2, Yeong-Geun Lee1, Tong Ho Kang1, Youn-Hyung Lee3, Dae Young Lee4,

young Seob Ko2, Nam-In Baek1*1Graduate School of Biotechnology & Department of Oriental Medicine Biotechnology,

Kyung Hee University, Yongin 17104, Republic of Korea, 2Korea Institute of Oriental Medicine, Daejeon 34054, Republic of Korea, 3Graduate School of Biotechnology &

Department of Horticultural Biotechnology, Kyung Hee University, Yongin 17104, Korea, 4Department of Herbal Crop Research, National Institute of Horticultural and Herbal

Science, RDA, Eumseong 27709, Republic of Korea

Citrus grandis (Rutaceae) is an evergreen shrub only distributed in Jeju island, Korea. Traditionally, C. grandis, which is called as “Dangyuji”, “Daeyuji”, has been used as a folk remedy for fever. And it was a precious fruit that must be used at various ceremonies in Jeju island. In recent studies, it was reported that C. grandis had two times higher organic acid content than C. junos and four times more vitamin C content than lemons. However, there is few study on chemical constituents and biological activities of C. grandis fruits. Therefore, the present study focused on the isolation and identification of active materials from the fruits of this plant, as well as evalutaion of protective effects on pancreatic islet (PI) damaged by alloxan in zebrafish (Danio rerio) larvae (AZL) for the isolated compounds. C. grandis fruits were extracted with aqueous methanol, and the concentrared extract was partitioned into EtOAc, n-BuOH, and H2O fractions. The repeated column chromatographies for EtOAc and n-BuOH fractions led to isolation of six isocoumarins and four flavonoids. Based on spectroscopic data, three new isocoumarins, citragrandin -C (1-3), three known coumarins (4-6), and four known flavonoids (7-10), were isolated from C. grandis fruits. Some compounds showed radical scavenging activities in DPPH and ABTS radical experiments and some compounds also exhibitied protective effects on PI damaged by AZL.

PNB-23

Cold Atmospheric Plasma induces Immunogenic Cell Death in Cancer Cells

Yongdae Yoon, Seung Joon Beak*

Seoul National University, College of Veterinary Medicine

Cancer is the second leading cause of death in the USA, and conventional therapeutic approaches including chemo/radio therapy may not improve the current crisis. We need either new technologies, concepts, or combination therapies to improve the current situation. Immunotherapeutic approach has been focused on cancer treatment because cancer drugs exhibit resistance on cancer cells. Recently, a non-thermal atmospheric plasma (or cold atmospheric plasma, CAP) was introduced and designed not only for denaturing cells and tissues, but also for operating under the threshold of thermal damage and for chemically inducing a specific response or modification. Plasma approach to the cancer patients is considered as a potential treatment on cancer therapy. In this study, we find Cold Atmospheric Plasma (CAP) induced high-mobility group box 1 (HMGB-1) protein expression and increased HMGB-1 secretion. Also, CAP induced localization of calreticulin (CRT) at cell membrane from cytoplasm. In addition, CAP induces cancer cell apoptosis through immunogenic cell death. Our data indicates CAP is a potential application of cancer therapy.


204

PNB-28

Effects of triterpenoid Alisol-F on human 5-hydroxytryptamine 3A and α3β4 nicotinic acetylcholine receptor channel activity



Alisma Rhizome is a known tradition medication, which has been used for its diuretic, hypolipidemic, anti-diabetic, and anti-inflammatory purposes for thousands of years. The primary compounds of Alisma Rhizome are protostane type triterpenes, such as Alisols A, B or C. We previously demonstrated that Alisol derivatives (Alisols A, B, and C) have inhibitory effects on 5-hydroxytryptamine 3A (5-HT3A) currents.1 In this study, we tested the effects of a new triterpene, Alisol-F, on human 5-HT3A and a3b4 nicotinic acetylcholine (nACh) receptor channel currents by using Xenopus oocytes expressing these channels. Co-application of Alisol-F inhibited 5-HT3A and a3b4 nACh receptor-mediated inward peak currents. The inhibitory effect of Alisol-F on 5-HT and ACh-induced inward peak currents occurred in a reversible and concentration-dependent manner. The half maximal inhibitory concentrations (IC50) of Alisol-F were 79.4±11.0 and 21.2±6.0 mM for the 5-HT3A and a3b4 nACh receptors, respectively. In addition, the inhibition of I5-HT and IACh by Alisol-F occurred noncompetitive and voltage insensitive manner. Taken together, these results show that Alisol-F may regulate 5-HT3A and a3b4 nACh receptors channel expressed in Xenopus oocytes.

PNB-25

Development of Novel GUL-based Multivalent Inhibitors for Increased Affinity of Prostate Cancer

Young-Do Kwon, Hee-Kwon Kim*


Prostate cancer is one of the most popular cancers in men, and main causes of cancer death. Prostate-specific membrane antigen (PSMA) is a transmembrane, 750 amino acid type II glycoprotein, which is upregulated on prostate cancers. It was knon that PSMA is overexpressed in most prostate cancers including metastatic tumors. Thus, PSMA is a promising target for developing novel pharmaceuticals for diagnosis and treatment of the disease. Herein, novel type of multivalent PSMA inhibitors containing PSMA target key structure, a glutamate-urea-lysine (GUL) structure, were synthesized, and imaging probes was attached to the newly prepared PSMA inhibitor moiety. Several examinations were carried out to assess the properties of inhibitors, and the result suggested that novel multivalent inhibitors can be promising agents to visualize prostate cancer.

PNB-26

A Molecular Basis For the Inhibition of Transient Receptor Potential Vanilloid Type 1 by Gomisin A

Chaelin Kim, Junho Lee*


Transient receptor potential (TRP) channels play an essential role as environmental sensors in primary afferent neurons. The transient receptor potential vanilloid type 1 (TRPV1) channel, also known as the capsaicin receptor, is critical to chemical and physical sensation, and is regulated by anesthetic chemicals through an unknown mechanism. In the present study, we investigated the effect of gomisin A on human TRPV1 channel activity. Schisandra chinensis contains bio-active compounds such as the gomisin derivatives and their related compounds. Co-application with gomisin A inhibited the capsaicin-mediated inward peak current. This inhibitory effect of gomisin A on capsaicin-induced inward current showed concentration-dependence, and was reversible. The half maximal inhibitory concentration of gomisin A was 62.7±8.4 µM. In addition, this inhibition occurred in a non-competitive and voltage-insensitive manner. Furthermore, molecular docking studies of gomisin A on TRPV1 showed it interacted predominantly with residues at cavities in the segment 1 and 2 of each subunit. Four potential binding sites for this ligand in the extracellular region at sensor domain of TRPV1 channel were identified. In summary, this study revealed the molecular basis for the interaction between TRPV1 and gomisin A, and provides a novel potent interaction ligand.

PNB-27

The regulatory effect of triterpenoids on α3β4 nicotinic acetylcholine receptor activity

Shinhui Lee, Junho Lee*


Alisma Rhizomes (SAM) is used as a diuretic, hypolipidemic, anti-diabetic and anti-inflammatory agent in traditional East-Asian medicine. In this study, we tested the effect of Alisma Rhizomes on the α3β4 nicotinic acetylcholine (nACh) receptor channel current in Xenopus oocytes. The acetylcholine-induced inward peak current (IACh) was measured with the two-electrode voltage-clamp technique. This experiment shows that the α3β4 nACh receptor cRNA injected into occytes followed by co-application with Alisma Rhizomes inhibited IACh in a noncompetitive or voltage insensitive condition. The half maximal inhibitory concentration (IC50) of Alisma Rhizomes was 12.5±3.4 mg/ml and the Vmax was 55.4 ±4.7. Protostane-type triterpenoids are the main active ingredient of Alisma Rhizomes (Alisol A, Alisol B, Alisol B 23-acetate, Alisol C 23-acetate). Altogether, our research shows that protostane-type triterpenoids may modulate the α3β4 nACh receptors expressed in oocytes in a reversible, concentration dependent and non-competitive manner. Furthermore, this modulation of the nACh receptor activity by protostane-type triterpenoids could underlie the pharmaceutics actions of Alisma rhizome.

Poster Presentation


Poster Presentation

PNB-32

Improvement of identification assay and HPLC-DAD method for the simultaneous determination of three marker compounds in Jaeumganghwatang extract granules and soft∙dried extract

Ka-Yeon Baek1, Hwa-Dong Lee2*1Department of Korean Medicine Development, Korean Medicine R&D Team 2,

2Department of Korean Medicine Development, Strategic Planning Team

Jaeumganghwatang (JGT) was a well-known one of the major traditional herbal medicines wildly used in the treatment of chronic bronchitis and inflammatory diseases. JGT was consisted of twelve medicinal herbs. However, the present procedure in Korean Herbal Pharmacopoeia (KHP) were treated many kind of preparation for each herbs. This study was intended to improve chemical analysis method for quality control of herbal medicines by high-performance liquid chromatography and thin layer chromatography (TLC). To develop and validate simultaneous determination method, C18 column (5.0 μm, 4.6 mm×250 mm) was used by gradient elution. The mobile phase consisted of acetonitrile and water containing 0.05% phosphoric acid at a flow rate 1.0 mL/min. The UV wavelength was set at 240. Method validation was accomplished by specificity, repeatability test and precision test. The relative standard deviations (RSD) value of precision test, intra-day and inter-day tests were less than 1.75% and 3.48%. Also this developed method was applied to commercial JGT extract granule products and the contents of three compounds ware consistent with the criteria range in Korean Herbal Pharmacopoeia (KHP). This results suggested the develop method was simple, effective and could be readily utilized as a quality control method for commercial JGT products.* This research was supported by a grant (16172MFDS230) from Ministry of Food and Drug Safety in 2019.

PNB-29

Cosmetic Hydrogel Maskpacks with Cellulose

Hong-Joo Byun, Hyang-Yeol Lee*


Hydrogels are homogeneous materials with amorphous structure for considering various properties. Due to their versatile properties, hydrogels have shown with many applications such as chemical actuators, surface coating, drug delivery, lubrication as well as cosmetic materials. To use as cosmetic products, hydrogels are often needed to design for the development of the tough hydrogels due to poor mechanical properties of natural polymers with non-covalent bonds. Various types of cellulose have been added to hydrogels to investigate the properties of hydrogels. Among them, when cotton fibers was added to hydrogel, tensile strength of hydrogel sheets were significantly increased by over 20% as well as showing high moisturizing effect. Hydrogels containing cellulase-treated cotton fibers showed the best effect on retaining moisture content increasing up to 380% in comparison with the one containing untreated cotton as well as excellent dispersibility.

PNB-30

Cisplatin(IV) Complexes with Short Fatty Acids

Hyang-Yeol Lee*


Although cisplatin has been one of the world’s best selling anticancer drugs and has become one of the most successful anticancer drugs being active in a series of solid tumors, the use of cisplatin in cancer treatment is often limited by resistance of cancer cells to the medicine. We have synthesized cisplatin derivatives with short fatty acids that are needed to have the ability to kill target cells selectively in a short time before drug resistance develops. Various PDK inhibitors were introduced on platinium complex as potential anti-cancer agents and charaterized their structures by nmr and mass spectrometer. Those platinum complexes showed strong potency against MCF-7 cell line which is cisplatin resistance cancer cells. A series of cisplatin complexes has shown cell viabilities against MCF-7 at 50 μM concentration up to 39%, 54% and 84% respectively.

PNB-31

Induction of Cancer Cell-Selective Apoptosis by Autocrine Motility Factor

Hee Sung Park*

Dept. Biotechnology, Daegu Catholic University

In cell competition, winner cells may produce diffusible killing signals to induce loser cell death. In this study, serum-free conditioned medium (CM) of cancer cells was tested for its effect on the growth of various cancer cells. For example, HeLa CM significantly inhibited A549, MCF-7, and AsPC-1 but little affected HL-60, SKOV3, or SNU-484. HeLa CM was fractionated through column chromatography and autocrine motility factor (AMF) was finally disclosed to be responsible for cancer cell growth inhibition. Indeed, Hela CM was no more active after it was pretreated with erythrose-4-phosphate or anti-glucose-6-phosphate isomerase (GPI) antibody. GPI, a housekeeping cytosolic enzyme, plays an important role in glycolysis and gluconeogenesis. Identical to GPI, extracellular AMF elicits mitogenic and motogenic functions and induce proliferation, differentiation, and survival of various cancer cells. HeLa rAMF was shown to significantly downregulate HER2/Akt and also AMFR/Akt signaling pathway and to induce cancer cell-selective apoptosis. Highly interestingly, each of AMFs cloned from various cancer cells including HeLa, A549, AsPC-1, Hep G2, MCF-7, SKOV3, and HT-29 was determined to have different cancer cell-selective apoptosis inducing characteristics. Results of this study provided good evidence that AMF could probably act as a diffusible killing signal in cell competition. More importantly, AMFs from various cancer cells may suggest a novel way to develop cancer therapeutics.


206

PNB-36

Effects of a combination of herbal extracts on non-alcoholic fatty liver disease (NAFLD) in mice fed a high-fat diet

Doo Jin Choi, Bo-Ram Choi, Dae Young Lee, Young-Seob Lee, Geum Soog Kim*

Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA

Non-alcoholic fatty liver disease (NAFLD) is a common chronic liver disease characterized by an abnormal accumulation of more than 5% fat by liver weight. In this study, we investigated whether a combination of two herbal extracts, called ALM, had hepatoprotective and synergistic effects against high fat diet-induced NAFLD in mice. Two medicinal herbs (AM and LE) were extracted by a heat reflux method with aqueous fermented ethanol, respectively, and then mixed to prepare ALM. The mice were induced with NAFLD in a high-fat diet for 7 weeks, and then were orally administered with ALM for 7 weeks. To clarify the effects of ALM on NAFLD in mice, it was assessed by measuring the liver weight and levels of triglycerides (TG) and glucose in serum. As a result, ALM groups were significantly decreased liver weight compared to the high-fat diet (HFD) group (p<0.001). In addition, ALM was also reduced the levels of TG and glucose in serum compared with HFD group, respectively (p<0.001). These results showed that ALM was more effective than the AM or LE treated group in a same dose. The results of this study suggest that ALM has protective effects on high-fat diet induced NAFLD in mice, based on synergistic action, and it might be a good candidate of functional materials for improving NAFLD.

PNB-33

Effect of Different Plant Growth Regulators (PGRs) on Propagation from Multiple Egg-Shape Micro-Tubers (MEM)

Culture of Pinellia Ternata (Thunb.) Breit. Tuber

Yong-Goo Kim1, Richard Komakech1,2, Youngmin Kang1,2*1Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, 111 Geonjae-ro, Najusi, Jeollanam-do 58245, Republic of Korea, 2University of Science &

Technology (UST), Korea Institute of Oriental Medicine Campus, Korean Medicine Life Science Major, Daejeon 34054, Republic of Korea

Pinellia ternata (Thunb.) Breit. is a perennial plant belonging to the genus Neptune, and it breeds well on a barren soil. In this study, we investigated the bulk growth multiple egg-shape microtubers (MEM) using tissue culture of these P. ternata tubers. The small tubers of P. ternata were investigated for the formation of small tubers under various plant growth medium conditions. As a result, the number of tuber formation at the highest point in LS medium was confirmed. When the small tuber organisms corresponding to the concentration of sugar were investigated on the basis of the LS medium in which the number of small tuber formation was the highest, 6% of the sugar in the living organism of the tubers at the concentration of 5%. It was confirmed that it was displayed higher than sugar. IAA (Indole-3-acetic acid) showed the highest cytogenetic result in combination with 0.5 mg/L and 1 mg/L of BAP (6-Benzylaminopurine), when we examined the cytopathic effect of cytokinin combination with auxin, a plant growth hormone. It is conceivable that the system optimized for large-scale in-machine propagation and soil purification of multiple egg-shape microtubers (MEM) used for pharmaceutical sites.

PNB-34

A review on the botanical aspects, phytochemical contents, and pharmacological activities of Warburgia ugandensis

Denis Okello1,2,3, Richard Komakech1,3,4, Motlalepula Gilbert Matsabisa5, Youngmin Kang1,3*

1Korean Convergence Medicine Major, University of Science and Technology (UST), Republic of Korea, 2Gombe Secondary School, P. O. Box, 192, Butambala/Mpigi, Uganda,

East Africa, 3Herbal Medicine Research Division, Korea Institute of Oriental Medicine (KIOM), Yuseongdae-ro, Yuseong-gu, Daejeon 34054, Republic of Korea, 4Natural

Chemotherapeutics Research Institute, Ministry of Health, Kampala, Uganda, 5Department of Pharmacology, Faculty of Health Sciences University of Free State, Bloemfontein 9300,

South Africa

Warburgia ugandensis Sprague (Family Canellacea) commonly known as Ugandan greenheart or pepper bark tree, is a highly valued medicinal plant in traditional medicine with a broad spectrum of antimicrobial activity whose parts especially the leaves and stem bark have for long been used in the treatment and management of many diseases and health conditions such as stomachache, cough, toothache, fever, malaria, oral thrush, measles, and diarrhea in African communities where the plant occurs. This review focused on the phytochemical contents, medicinal uses, and antimicrobial activities of W. ugandensis based on published peer reviewed articles. This review established that the high therapeutic value of W. ugandensis is attributed to the abundance of drimane sesquiterpenes in its stem bark and leaves. These phytochemicals have also made the plant to have potent antibacterial and antifungal activities. However, more pre-clinical and clinical trials need to be done to further validate the traditional medicine applications of W. ugandensis for possible drug discovery. Due to its high demand, W. ugandensis has been over exploited and hence its population is in drastic decline. Consequently, there is need for development of advanced and more rapid propagation techniques to increase its population and distribution in its natural environment to meet the ever-increasing demand.

PNB-35

Secondary Metabolites Production Based on Adventitious Root Cultures in Medicinal Plants

Endang Rahmat1,2, Youngmin Kang1,2* 1University of Science & Technology (UST), Korea Institute of Oriental Medicine, Korean

Convergence Medicine major, Daejeon 34054, Republic of Korea, 2Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, 111Geonjae-ro,

Naju-si, Jeollanam-do 58245, Republic of Korea

The increasing global demand for bioactive compound production of medicinal plant must be offset by a qualified production technology. One approach has been widely used to welcome this challenge is the utilization of adventitious root culture as a propagation strategy. Here, this study highlights the application of adventitious root culture in many medicinal plants that have been scientifically studied worldwide. Adventitious roots induced under sterile condition in growth regulator supplemented medium have shown high rate of proliferation, tremendous potentialities of accumulation and stable production of valuable secondary metabolites. Such parameters like medium composition, growth hormone type as well as elicitation technique through adventitious root culture in medicinal plants are become the main discussion. In addition to advances in plant cell culture, recent successes in large-scale bioreactor production of several plant secondary metabolites through adventitious root culture seems to be a promising approach. In vitro factors influencing scale up system such as culture medium properties, hormonal balance, gaseous composition, growth kinetics, inoculum density, and culture period are also highlighted in this study. In conclusion, the development of adventitious root culture for medicinal plant is highly useful due to its capability in vegetative propagation and germplasm preservation.

Poster Presentation


Poster Presentation

PNB-40

Hepatoprotective Effect of Platycodon grandiflorum Extract on Alcohol-Induced Liver Damage in Mice and HepG2 Cells

Jeon Hwang-Bo1, Dahye Yoon2, Seon Min Oh2, Bo-Ram Choi2, Youn-Hyung Lee3, Dae Young Lee2*

1Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea, 2Department of Herbal Crop Research,

National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, Republic of Korea, 3Department of Horticultural Biotechnology,

Kyung Hee University, Yongin 17104, Republic of Korea

We investigated the protective effect of Platycodon grandiflorum extract (PGE) on alcohol-induced liver damage (AILD) in vitro and in vivo. We used the intragastric feeding model to induce liver damage. Liver damage was induced by oral administration of 25% ethanol (EtOH) daily (5 g/kg) with or without PGE (50, 100, and 200 mg/kg) for 3 weeks. Liver damage was assessed by histopathologic examination, quantification of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels in serum, and antioxidant enzyme levels such as superoxide dismutase (SOD) and catalase (CAT) in liver tissues. The levels of AST and ALT were increased in EtOH-fed mice and were suppressed by PGE. The levels of SOD and CAT were decreased in EtOH-fed mice and were increased by PGE. The PGE was inhibited the production of nitric oxide (NO) in EtOH-treated HepG2 cells. The expression of pro-inflammatory mediators such as cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), interleukine-6 (IL-6) was significantly increased in EtOH-treated HepG2 cells and was decreased by PGE. Also, PGE increased markedly the expression of the hepatoprotective enzyme, hemeoxygenase-1 (HO-1) in EtOH-treated HepG2 cells. These results suggest that PGE has a protective effect against AILD and is a new therapeutic candidate for the prevention and treatment of AILD.

PNB-37

DYT-BE Suppress Proliferation and COX-2 Expression through the AKT pathway in HT-29

Ji-Eun Park, Eun-Kyung Son, Bo-Mi Kim, Jai-Hyun So, Hyo-Hyun Park*

Department of Korean Medicine Development, National Development Institute of Korean Medicine

Colorectal cancer (CRC) remains one of the most common malignancies in the world. Over-expression of cyclooxygenase-2 (COX-2) has been detected in colorectal cancers. The objective of our study was to investigate anti-cancer effects of Dangkwiyughwang-tang ethanol extract bioconverted by protease liquid coenzyme of Aspergillus kawachii (DYT-BE), compared to a non-bioconverted DYT (DYT-E), and determine the underlying mechanisms. DYT-BE (25-100 μg/ml) treatments inhibited proliferation, migration and COX-2 expression of HT-29 colon cancer cells and increased apoptotic cell death. DYT-BE treatment also decreased ERK and AKT phosphorylation. Treatment with LY294002 (an AKT inhibitor) decreased cell proliferation and COX-2 expression of HT-29 cells, while a combined treatment with LY294002 and DYT-BE (50 μg/ml) resulted in even further decreases in proliferation and COX-2. DYT-BE treatment also appeared to induce synergistic inhibitory effects of proliferation and COX-2 by suppression of AKT in HT-29.

PNB-38

Inhibitory effect of the halophyte Ligustrum japonicum on matrix metalloproteinases-2 and 9 in HT-1080 cells

Junse Kim1, Minjeong Park2, Youngwan Seo2*1Ocean Science & Technology School, Korea Maritime & Ocean University, 2Division of

Marine Bioscience, Korea Maritime & Ocean University

Matrix metalloproteinases (MMPs) are associated with a variety of physiological and pathological processes such as cancer, in particular, tumor angiogenesis, sclerosis, arthritis, inflammation and vascular disease. This study evaluates the inhibitory effect of Ligustrum japonicum on MMP-2 and MMP-9 activity in HT-1080 human fibrosarcoma cells using gelatin zymography and reverse transcription polymerase chain reaction (RT-PCR). L. japonicum was extracted twice for 24 h with dichloromethane (CH2Cl2) and methanol (MeOH). The combined crude extracts were partitioned between CH2Cl2 and water. The organic layer was further fractionated into n-hexane, 85% aqueous methanol (85% aq.MeOH) and the aqueous layer was fractionated into n-butanol (n-BuOH) and water, successively. Of the fractions, 85% aq.MeOH fraction showed the significant inhibitory effect on MMP-2 and MMP-9.

PNB-39

Inhibitory capability of Artemisia princeps on matrix metalloproteinases-2 and -9 in HT-1080 cells

Minjeong Park1, Junse Kim2, Yeongwan Seo1*1Division of Marine Bioscience, Korea Maritime & Ocean University, 2Ocean Science &

Technology School, Korea Maritime & Ocean University

Dried samples of Artemisia princeps were extracted four times: twice with methylene chloride (CH2Cl2) and twice with methanol (MeOH). These two crude extracts (CH2Cl2 and MeOH) were combined and then partitioned with CH2Cl2 and water. The aqueous layer was re-partitioned between n-butanol (n-BuOH) and water and then the organic layer was fractionated into n-hexane and 85% aqueous MeOH (85% aq.MeOH). Four fractions of A. princeps were tested for their inhibitory effects against matrix metalloproteinases (MMPs)-2 and -9 in HT-1080 human fibrosarcoma cells using gelatin zymography and reverse transcription polymerase chain reaction (RT-PCR). Of th fractions, n-hexane fraction showed the most significant inhibitory effect on MMP-2 and -9.


208

PNB-44

Antibacterial and Antioxidant activities of Parthenocissus tricuspidata extracts

Yu-Jin Oh1,2, Hae Jin Cho1, Hyun Sim Woo1, Yeong-Su Kim1*, Dae Wook Kim1*1Forest Plant Industry Department, Baekdudaegan National Arboretum, 2Advanced Bio

and Fermentation Convergence, BK21 Plus, Kookmin University

Parthenocissus tricuspidata distributed in Korea, china and japan is a woody vine that typically grows 3-50 feet or more. The leaves have been used as folk medicine in South Asia, for treating arthritis, jaundice, insect bites, and neuralgia. This study was carried out to analyze the effects on antibacterial and antioxidative activities of Parthenocissus tricuspidata extracts (PTE). PTE inhibited the growth Gram-positive bacteria, Staphylococcus aureus (KCTC 3881) by using an agar disc diffusion assay. The antioxidative activities of PTE were measured by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and 2,2’-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) assay. The IC50 values for DPPH and ABTS are 125.85 and 32.34 mg/ml, respectively. Thus, these results suggest that the extracts of P. tricuspidata could be used as a potential source for natural antioxidant.

PNB-41

The Inhibitory Effects of Manassantin B on VEGF-A-induced Lymphangiogenesis and Lymph Node Metastasis in an Oral

Sentinel Lymph Node Animal Model

Jeon Hwang-Bo, Mun Gyeong Bae, In Sik Chung*

Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea

The main factor that affects the prognosis of patients with oral squamous cell carcinoma (OSCC) is regional lymph node metastasis. Regional lymph node metastases usually occur via the sentinel lymph node (SLN), which is the first draining lymph node from the primary tumor. We investigated the effects of manassantin B (MB), a neolignan isolated from the roots of Saururus Chinensis, on VEGF-A-induced lymphangiogenesis and lymph node metastasis both in vitro and in vivo. MB inhibited the proliferation, tube formation, and migration of VEGF-A-treated human lymphatic microvascular endothelial cells (HLMECs). MB reduced in vivo lymphatic vessel formation in VEGF-A-stimulated Matrigel plug. To investigate the in vivo inhibitory effects of MB, we established an oral cancer sentinel lymph node animal model using BALB/c mice and oral squamous cell carcinoma SCCVII cells. We confirmed the inhibitory effects of MB on VEGF-A-induced lymphangiogenesis and sentinel lymph node metastasis in the animal model. MB suppressed the VEGF-A-induced phosphorylation of VEGFR-1 and VEGFR-2. In addition, MB reduced the activation of signaling factors such as FAK, PI3K, AKT, ERK1/2 and p38, involved in VEGF-A/VEGFR-1 and VEGFR-2 signaling pathway. Our results indicate that MB has the inhibitory effect on VEGF-A-induced lymphangiogenesis and lymph node metastasis and these suggest that MB can be a useful anti-tumor agent to restrict the metastatic spread of oral cancer.

PNB-42

Development and validation of a HPLC-DAD method for ethanolic extracts of Lespedeza bicolor roots

Hyun Sim Woo1,2, Yu Jin Oh1, Hae Jin Cho1, Min-Sung Lee1, Yeong-Su Kim1*, Dae Wook Kim1*

1Department of Forest Plant Industry, Baekdudaegan National Arboretum, 2Division of applied life Science, Institute of Agriculture and Life Science,

Gyeongsang National University

In this study, a simple and validated HPLC method for identifying and quantifying major substance in Lespedeza bicolor roots was developed. Xanthoangelol (XL), the main bioactive components in this plant, was isolated with column chromatography and used as the standard substances for quantitative analysis. The structural determination was elucidated by comparing their NMR spectral data with values reported in the literature. For validation, the specificity, linearity, precision, accuracy, detection limits, and quantification limits of XL was measured by HPLC. The results showed that the specificity was satisfied with retention time and diode array detector (DAD) spectrum by analysis of XL using HPLC. The limits of detection (LOD) for XL was 0.38 mg/ml. Recovery of XL was 98.66~102.99% with R.S.D values less than 1%. Intra-day and inter-day precisions of XL in L. bicolor were 98.66~102.56% and 98.78~102.98%, respectively. Therefore, this result suggest that the validated HPLC method could be a useful tool for the quality control of food or drug formulations containing L. bicolor.

PNB-43

Evaluation of antioxidant and antibacterial activities of Lamium album L. var. barbatum

Hae Jin Cho1,2, Yu-Jin Oh1, Hyun Sim Woo1, Yeong-Su Kim1*, Dae Wook Kim1*1Forest Plant Industry Department, Baekdudaegan National Arboretum, 2School of

Biological Sciences and Institute of Microbiology, Seoul National University

Lamium album L. var. barbatum is a perennial herb growing along forest edges. Its white flowers are characterized by a long-tubed labiate corolla and are open from April to July. In this study our objective was to evaluate the antioxidant antimicrobial activity of methanolic extracts of whole plant of L. album. The antioxidant activities were evaluated using DPPH, ABTS free radial scavenging and the antibacterial activity was evaluated by disc diffusion method and determination of minimum inhibitory concentration. The methanol extracts showed the highest free radical scavenging activity with IC50 values of 123.07 ug/ml in DPPH and 256.57 ug/ml in ABTS assay. The inhibition zones against Streptococcus mutans were ranged from 5– 10 mm. The results indicated that the methanolic extracts of L. album possessed antibacterial and antioxidant properties and could be an important source of natural compounds for development of new drugs.

Poster Presentation


Poster Presentation

PNB-48

Preventive effect of immmature Citrus unshiu extract on o-toluidine and 2-ethylanilineinduced urinary bladder toxicity

in Balb/c mice

Ji Hye Kim, Min Young Kim*


The toxicity of an unknown substance can be estimated by comparison with structurally similar compounds with known toxicity. While human bladder carcinogen 2-metylaniline (o-toluidine) has been studied, no studies concerned with the toxicity of 2-ethylaniline (2-EA) have been conducted in experimental animals. This study examined 2-EA toxicity compared to o-toluidine, and the potential protective effect of immature Citrus unshiu extract on toxicities of both anilines. Male Balb/c mice were administered orally with 0, 80, 160 mg/kg of either o-toluidine or 2-EA for 2 weeks. No substance-related mortality, but symptoms such as lethargy, slight tremor and hair loss and splenomegaly, as well as inflammation-associated fibrosis in the bladder as determined by histopathological examination were observed in o-toluidine and 2-EA-treated mice. Conversely, co-treatment with immature Citrus unshiu extract produced a marked decrease in clinical symptom and bladder inflammation associated with o-toluidine and 2-EA. The results of this study indicate that o-toluidine and 2-EA induce bladder toxicity, and Citrus unshiu extract offers a protective effect against o-toluidine and 2-EA-induced urinary bladder toxicity [Supported by Basic Science Research Program (2017R1D1A1B03028849, 2016R1A6A1A03012862 and 2014R1A1A2056292) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology, Republic of Korea].

PNB-45

Cultivated Wild Ginseng Attenuates Angiogenesis by Inhibiting the Phosphorylation of FAK and Src

Jin Soo Kim1, Jungeun Kim1, Jong Eel Park1, Dong Hee Kim2, Young Mi Seok1, Hyo Jung Kim1*

1Department of Korean Medicine Development Korean Medicine R&D Team 1, National Development Institute of Korean Medicine, Kyeongsan 38540, Republic of Korea,

2Department of Industrialization Guidance Team, National Development Institute of Korean Medicine, Kyeongsan 38540, Republic of Korea

Cultivated wild ginseng (CWG), called Asian or Korean ginseng is traditionally used in Korea and China and have been shown to possess a variety of therapeutic effects, including antineoplastic, antioxidant, and anticancer activities. However, Its effect on anti-angiogenesis has not yet been reported. In this study, we investigated the anti-angiogenic activities of a CWG in vitro. Our results suggested that CWG treatment dose-dependently inhibited cell migration, and formation of capillary-like structure of SVEC4-10 cells without cytotoxicity. Also, CWG treatment caused significant decrease in phosphorylation of focal adhesion kinase (FAK) and Src in a dose-dependent manner, demonstrating that the inhibitory effects of CWG on cell migration were mediated by disturbing the activation of focal adhesion kinase. Overall, these results suggest that CWG could be a potential therapeutic candidate to regulate angiogenesis.

PNB-46

Nematicidal Activity of Benzyloxyalcohol against Bursaphelenchus xylophilus

Junheon Kim1*, Jun Oh Park1, Su Jin Lee1,2, Mannyun Kim1

1Forest Insect Pests and Diseases, National Institute of Forest Science, 2Department of Life Sciences, Incheon National University

Pine wood nematode (PWN), Bursaphelenchus xylophilus causes devastating damage to pines worldwide. Control and prevention of pine wood disease (PWD) are primarily trunk injection of abamectin and emamectin benzoate. Previously we found the nematicidal activity of 2-(1-undecyloxy)-1-ethanol (monochamol), which is reported to be aggregation pheromone on its vectors, Monochamus species was as same to that of abamection. Since then, for developing new agent for controlling and preventing PWD using alkyloxyalcohols (ROR’OH), we synthesized alkyloxyalcohols, and reported their nematicidal activity against PWN and that the structure of alkyloxyalcohol affected to the nematicidal activity. Now we newly synthesized benzyloxyalocohols (A-H) and tested their nematicidal activity for development of new agent for PWD and for comparing their activity with alkyloxyalcohols. The nematicidal activity was different according to total carbon chain length in compounds. It may be due to the different position of oxy group in the compound. LC50 values of I and H were the lowest among the tested compounds. However, their nematicidal activity was much lower than that of monochamol and other alkyloxyalcohols. We will reported the nematicidal activity of benzyloxyalochols.

PNB-47

Antioxidant and Anti-inflammatory effects of Fermented Samsoeum by Bioconversion

Min A Kim, Han Saem Lee, Yu Mi Lim, So Hyun Chon, Eun Kyung Son, Jai Hyun So*

New Korean Medicine Team, National Development Institute of Korean Medicine

The aim of this study was to investigate the increase of antioxidant and anti-inflammatory activities of fermented samsoeum by bioconversion with Aspergillus kawachii. UPLC chromatogram showed a different pattern after bioconversion. Water extract of fermented samsoeum showed the highest 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity. Also, the fermented samsoeum extracts presented higher inhibitory effect on NO production than non-fermented samsoum extracts. From the results of this study, the samsoeum by bioconversion with Aspergillus kawachii showed higher antioxidant and anti-inflammatory activities than non-fermented samsoeum.


210

PNB-52

The hair growth promotion effects of Centipedegrass extract in vitro and in vivo

Fatuma Jumapili Ramadhani1,2, Seong Hee Kang1, Dong Ho Bak1, Sang Hun Park1, Hyoung Woo Bai1,2*

1Research division for Biotechnology, Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI), 2Radiation Biotechnology and Applied

Radioisotope Science, University of Science and Technology (UST)

Centipedegrass is the medicinal herb plants which possess several biological activities including anti-cancer, anti-diabetic, anti-inflammation and anti-fungal. However, the role of Centipedegrass extract (CGE) on the hair growth effects is not discovered yet. Therefore, the current study aimed to unravel the hair regrowth effects of CGE, and the mechanism of hair cycle progression. The effects of CGE on cell viability and genes related to hair induction in keratinocytes (HaCaT) cells and Human hair dermal papilla cells (hHDPC) were examined. Furthermore, the histological assessment was conducted after topical application of CGE on CB57L/6 mice skin for 21 days. CGE increased cell viability of hHDPC and HaCaT cells and upregulates hair growth related genes especially β-catenin, VEGF and Versican. Furthermore, CGE administration on the mice skin, allowed transition of telogen to anagen hair follicle. Also, the immunohistochemistry assay showed that CGE upregulated sonic hedgehog, β-catenin and Ki-67 expression in the hair follicle. These results demonstrate that CGE modulate the hair growth cycle progression and may have the potential benefits for hair loss treatment.

PNB-49

Skin aging effect of Umbilical cord blood-derived Stem Cell P on human fibroblast

Jun-Seok Baek1*, Jua Kim1, Su-Young Kim1, Seung Hye Kang2, Se-Eun Jin2, Eunkyung Hong2, Jeong-Kyun Yoo1, Chae Yeon Kim1, Young Soo Kim2*

1Material Development Team, Materials Science Research Institute LABIO Co., Ltd., 2Materials Science Research Institute, Materials Science Research Institute LABIO Co., Ltd.

Stem cell extract have been widely studied because of their excellent positive therapeutic effect on skin aging. USC-CM (Umbilical cord blood-derived Stem Cell Conditioned Media) contains plenty of growth factors secreted by differentiating stem cells. It is an attractive material, but the shelf life is short and difficult to use due to the risk of contamination and stability. To improve the preservation and usability of the umbilical cord blood stem cell cultivation fluid, we have established a method of using a freeze-dried powder. USC-P (Umbilical cord blood-derived Stem Cell Powder) is a freeze-dried powder with improved applicability and storage. The aim of the present study was to develop a new functional agent for cosmetics ingredient, we investigated the effect of USC-P on skin aging. We evaluated the matrix metalloproteinases-1 (MMP-1) expression on fibroblasts irradiated with UVB, and wound healing potential. The USC-P reduced UVB-induced MMP-1 expression and accelerated wound healing effect. These results suggest that USC-P may have beneficial applicability as a cosmetic ingredient candidate for an anti-skin aging agent.

PNB-50

Anti-inflammatory effect of solvent fractions in the aerial part and bulb of Lilium hansonii leichtlin

Hyun Jeong Kim, Su-Yeon Cho, In Guk Hwang, Byung Soon Hwang, Jeong-Sook Choe, Hwan-Hee Jang*

National Institute of Agriculural Science, Rural Development Administration (RDA)

Lilium hansonii Leichtlin is a Korean endemic species the grows only in the Ullengdo and belongs to the Matagon section of the genus Lilium. L. hansonii is mainly used for gardening (flowers) and food (bulbs). Some Lilium species are a rich source of chemical diversity and have been reported to be used as traditional medicines, but studies on their effectiveness are lacking. Our previous studies have confirmed the anti-thrombotic and anti-inflammatory effects of the 70% fermented ethanol extracts of L. hansonii. In this study, L. hansonii was fractionated with a solvent, such as hexane, chloroform, ethyl acetate, butanol, and water, to identify the major extract fractions with the anti-inflammatory effects. L. hansonii fractions were evaluated the anti-inflammatory ability by evaluating inhibitory effect on NO, PGE2, IL1beta and IL6 production in LPS-stimulated Raw264.7 cells. As a result, L. hansonii fractions inhibited the inflammatory response induced by LPS. Especially, the production of NO and inflammatory cytokines were suppressed in the chloroform fraction. The findings of this study will be the basis for future research to identify anti-inflammatory compounds of L. hansonii.

[This work was carried out with the support of “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ0142019)” Rural Development Administration, Republic of Korea.]

PNB-51

Delphinidin enhance radio-therapeutic effect via autophagy induction and JNK/MAPK pathway activation in

non-small cell lung cancer

Seong Hee Kang1, Dong Ho Bak1, Yong Dae Park1, Byung Yeoup Chung1, Hyoung Woo Bai1,2*

1Research Division for Biotechnology, Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI), 2Radiation Biotechnology and Applied

Radioisotope Science, University of Science and Technology (UST)

Delphinidin is a major anthocyanidin compound found in various vegetables and fruits. It has anti-oxidant, anti-inflammatory and various other biological activities. In this study, we explored the biological activities of delphinidin that were related to autophagy in radiation exposed non-small cell lung cancer (NSCLC). Radiosensitizing effects were assessed in vitro by treating cells with subcytotoxic dose of delphinidin (5 uM) prior to γ ionizing radiation (IR). We found that treatment with delphinidin or IR induced NSCLC cell death in vitro, but the combination of delphinidin pre-treatment and IR was more effective than either agent alone, yielding radiation enhancement ratio (REF) of 1.54 at 50% lethal dose (LD50). Moreover, combined treatment with delphinidin and IR, enhanced apoptotic cell death, suppressed mTOR pathway and activated JNK/MAPK pathway. Delphinidin inhibits the phosphorylation of PI3K, AKT, mTOR, and increased the expression of autophagy induced cell death associated-protein in radiation-exposed NSCLC cells. In addition, the phosphorylation of JNK was upregulated by pre-treatment of delphinidin in radiation exposed NSCLC cells. Collectively, these results showed that delphinidin acts as a radiosensitizer through autophagy induction and JNK/MAPK pathway activation enhancing apoptotic cell death in NSCLC cells.

Poster Presentation


Poster Presentation

PNB-56

Phytochemical constituents of the whole plants of Aster koraiensis

Ji-Young Kim1, Ranhee Kim1, Yutong Qi1, So-Ri Son2, Keon-Tae Park1, Dae Sik Jang1*

1Department of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Korea, 2Department of

Biomedical Science and Technology, Graduate School, Kyung Hee University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Korea

Aster koraiensis Nakai is a perennial herb that belongs to Compositae and is an endemic plant to Korea. The young leaves and stems of A. koraiensis have long been used to food source in Korea. The roots of A. koraiensis have been used to traditional Korean medicine to treat chronic bronchitis, antitussive, and antibacterial activities. Repeated chromatography of a 95% EtOH extract of the whole plants of A. koraiensis led to the isolation and characterization of two new sesquiterpenoids (1 and 4), a new phenolic compound (6), along with three sesquiterpenoids (2, 3, and 5), two caffeoylquinic acids (7-8), three flavonoids (9-11), two ionones (12 and 13), one pyrone (14), one benzofuran (15), one polyacetylene glucopyranoside (16), and five polyacetylenes (17-21) with previous known structures. The structures of the new compounds (1, 4, and 6) were determined by interpretation of spectroscopic data, particularly by 1D- and 2D-NMR spectral data analysis.

PNB-53

Antimicrobial activities of an oxygenated cyclohexanone derivative isolated from Amphirosellinia nigrospora JS-1675

against various plant pathogenic bacteria and fungi

Hoa Thi Nguyen1, Soon Ok Kim2, Nan Hee Yu1, Hyeokjun Yoon2, Chang-Hwan Bae2, Joo Hong Yeo2, Khanh Duy Le1, Van Thi Nguyen1,

Jongmoon Kim1, Ae Ran Park1, Jin-Cheol Kim1*1Department of Agricultural Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, 77 Yongbong-ro,

Buk-gu, Gwangju 61186, Republic of Korea, 2Biological and Genetic Resources Assessment Division, National Institute of Biological Resources, 42 Hwangyeong-ro, Seo-gu, Incheon

22689, Republic of Korea

During the screening of our research for bioactive secondary metabolites from microorganism, we found that one strain, Amphirosellinia nigrospora JS-1675 showed strong in vitro antibacterial activity against Ralstonia solanacearum with a minimum inhibition concentration (MIC) of 5% of fermentation broth filtrate. The active compound 1 was identified as (4S,5S,6S)-5,6-epoxy-4-hydroxy-3-methoxy-5-methyl-cyclohex-2-en-1-one by mass and NMR spectroscopy. The in vitro antibacterial activity of 1 and EtOAC layer were tested against 14 phytopathogenic bacteria. The two samples showed similar antibacterial activity with MIC value of 31.2-500 µg/ml except Pseudomonas syringae pv. lachrymans did not show any inhibition. Additionally, this compound also reduced the mycelia growth of plant pathogenic fungi except C.coccodes and F.gramineearum in a dose-dependent manner. Both compound 1 and the EtOAc layer reduced bacterial leaf spot disease in detached peach leaves. They also suppressed the development of bacterial wilt on tomato seedling by 84.6% and 76.9% at 125 µg ml-1, 92.3% and 88.5% at 250 µg ml-1, and 100.0% respectively, seven days after inoculation. This is the first report on the occurrence of (4S,5S,6S)-5,6-epoxy-4-hydroxy-3-methoxy-5-methyl-cyclohex-2-en-1-one in A. nigrospora JS-1675 and their efficacy against phytopathogenic bacteria and fungi. The high percentage of inhibition activity against plant bacteria suggest that this compound may be good sources for potential natural products.

PNB-54

Biological control of various bacterial diseases by Bacillus methylotrophicus DR-08 producing oxydifficidin and difficidin

Seong Mi Im1, Nan Hee Yu1, Hee Won Joen1, Soon Ok Kim2, Hae Woong Park3, Ae Ran Park1, Jin-Cheol Kim1*

1Department of Agricultural Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, 77 Yongbong-ro,

Buk-gu, Gwangju 61186, Republic of Korea, 2Biological and Genetic Resources Assessment Division, National Institute of Biological Resources, 42 Hwangyeong-ro, Seo-gu, Incheon

22689, Republic of Korea, 3R&D Division, World Institute of Kimchi, 86 Kimchi-ro, Nam-gu, Gwangju 61755, Republic of Korea

Bacillus methylotrophicus DR-08 strongly inhibited the cell growth of various phytopathogenic bacteria. This study aimed to identify the antibacterial metabolites and to evaluate the efficacy of the strain as a biocontrol agent for the control of bacterial diseases. The butanol extract of DR-08 broth culture completely inhibited the growth of 14 phytopathogenic bacteria with minimum inhibitory concentration (MIC) values of 1.95– 500 μg/mL. Xanthomonas oryzae pv. oryzae was most sensitive at with a MIC value of 1.95 μg/mL. Through the bioassay-guide fractionation and instrumental analyses, two metabolites were isolated and identified as difficidin and oxydifficidin. Both metabolites inhibited the cell growth of most phytopathogenic bacteria tested and oxydifficidin generally showed stronger antibacterial activity than difficidin. The difficidin biosynthetic gene cluster was confirmed by polymerase chain reaction of partial region genes (dfnA, dfnG, dfnM). The 30% suspension concentrate formulation of DR-08, at 500-fold, effectively suppressed the development of tomato bacterial wilt in pot experiment and field experiments. It also effectively reduced the development of leaf symptoms of bacterial spot on peach and red pepper. This study suggests that B. methylotrophicus DR-08 can be used as a good biocontrol agent for the control of various plant diseases caused by phytopathogenic bacteria.

PNB-55

Design, synthesis, and biological evaluation of 1-aryl-5-(2-(styryl)phenyl)penta-1,4-dien-3-one

Seunghyun Ahn1, Miri Yoo1, Jihyun Park2, Youngshim Lee2, Hyeok Lee2, Kyungrai Kang2, Dongsoo Koh1, Soon Young Shin3, Young Han Lee3,

Yoongho Lim2*1Department of Applied Chemistry, Dongduk Women’s University, Seoul 02748, Korea,

2Division of Bioscience and Biotechnology, BMIC, Konkuk University, Seoul 05029, Korea, 3Department of Biological Science, Konkuk University, Seoul 05029, Korea

Chalcones containing α,β-unsaturated carbonyl group have been known to show anticancer activities. Curcumin also shows anticancer activity, but because of its poor potency and bioavailability, further modification is required. We modified its diene-dione moiety to dienone and combined with chalcone. As a result, 1-aryl-5-(2-(styryl)phenyl)penta-1,4-dien-3-ones were designed and twenty-six derivatives were synthesized. To evaluate their anticancer activities, cell growth inhibitory effects against HCT116 colon cancer cell lines were measured using the clonogenic long-term survival assay. Since it has been reported that one of targets of curcumin is the microtubule, we tested whether 1-aryl-5-(2-(styryl)phenyl)penta-1,4-dien-3-one can inhibit the polymerization of tubulin. In addition, the cell cycle arrest was observed at the G2/M phase. Here, we report the NMR and HR/MS spectroscopic data to identify the synthetic derivatives, and their biological data obtained from the clonogenicity, flow cytometry, and Western blot analysis.


212

PNB-60

Design and Preparation of Novel Oseltamivir Derivatives as Potential Therapy Agents Against Influenza Virus

Hee-Kwon Kim*


Influenza A virus was reported as one of Orthomyxoviridae families. A lot of people are influenced from influenza virus for a long time, and many people of the earth have been died though global influenza pandemics. Due to human health concern, scientists made a wide efforts to develop effective treatments of influenza virus infection. Oseltamivir, the first orally available drug, was widely used after it was marketed in 2000. With the interested in development of novel anti-influenza drugs, we designed new type of oseltamivir derivatives, and their neuraminidase inhibition constants were examined via computer calculation to find new promising structures to treat influenza virus. From the calculation data, new potential oseltamivir derivatives were prepared from commercially available inexpensive starting materials. Our strategy will provide useful approach for the development of novel potent anti-influenza agents.

PNB-57

Neuroprotective effect of Myristargenol A on the apoptosis of glutamate-induced mouse hippocampal HT22 cells

Jung Soo Park, Ki-Young Kim*

Kyung Hee University

Myristargenol A (MG) is known to have anti-cancer effects. The purpose of this study was to determine the neuroprotective effect of MG against glutamate-induced neuronal cell death.Glutamate is the important excitatory neurotransmitters in the mammalian central nervous system (CNS) at low concentrations but glutamate cause excitotoxicity and apoptosis leading to neurodegenerative diseases at high concentrations.The neuroprotective effect of MG was evaluated by analyzing mitochondrial membrane potential (ΔΨm) change, Bcl-2 and Bax expression ratio, caspase pathway activation and mitogen-activated protein kinases (MAPK) activity in HT22 cells.MG (10 μM) significantly decreased glutamate-induced death of HT22 cells (113.6 ± 2.3% relative neuroprotection) and helped ATP production by preventing mitochondrial membrane disruption.Also, MG increased expression ratio of Bcl-2 and Bax, which are important in mitochondria-mediated apoptosis, and inhibited cleavage of proteins in that pathway (caspase-9, caspase-3, PARP).The phosphorylation of p38 and JNK was promoted by the increase of oxidative stress due to the high concentration of glutamate, but the phosphorylation level was reduced by treatment with MG. In contrast, phosphorylation of ERK involved in cell growth and proliferation was increased.These results suggest that MG have a neuroprotective effect.

PNB-58

Acyclic Triterpenoids from Alpinia katsumadai Inhibit IL-6-Induced STAT3 Activation

Ye-Ji Park, Yu-Mi Yang, Seung Woong Lee, Mun-Chual Rho*


The seeds of Alpinia katsumadai yielded two new acyclic triterpenoids, 2,3,6,22,23 -pentahydroxy-2,6,11,15,19,23-hexamethyl-tetracosa-7,10,14,18-tetraene (3) and 2,3,6,22,23-pentahydroxy-2,10,15,19,23-hexamethyl-7-methylenetetracosa-10,14,18-triene (4), as well as two known compounds, 2,3,22,23-tertrahydroxy-2,6,10,15,19,23-hexamethyl-tetracosa-6,10,14,18-tetraene (1) and 2,3,5,22,23-pentahydroxy-2,6,10,15,19,23-hexamethyl-tetracosa-6,10,14,18-tetraene (2). The absolute configurations of 2 and 3, which were determined by means of a modified Mosher’s method, are suggested as (3R; 5S; 22R) and (3R; 22R), respectively. Compounds 1-4 inhibited IL-6-induced JAK2/STAT3 activity in a dose-dependent fashion, with IC50 values of 0.67, 0.71, 2.18, and 2.99 μM. Moreover, IL-6-stimulated phosphorylation of STAT3 was significantly suppressed in U266 cells by the administration of A. katsumadai EtOH extract and Compounds 1 and 2. These results suggest that major phytochemicals, Compounds 1 and 2, obtained from A. katsumadai may be useful candidates for designing new IL-6 inhibitors as anti-inflammatory agents.

PNB-59

Synthesis of thienopyrimidine derivatives as inhibitors of STAT3 activation induced by IL-6

Eun-Jae Park, Hee Ju Lim, Seung Woong Lee, Mun-Chual Rho*


A series of thienopyrimidine compounds (6Aa-g and 6Ba-d) were synthesized and characterized by NMR spectroscopy and mass spectrometry. These compounds (6Aa-g and 6Ba-d) potently inhibited STAT3 expression induced by IL-6 in a dose-dependent manner with IC50 values of 5.73-0.32 µΜ. Among the prepared thienopyrimidine derivatives, 6Aa, 6Ab, 6Ba and 6Bc significantly suppressed the phosphorylation of STAT3 and ERK1/2 stimulated by IL-6 in Hep3B cells. Furthermore, the synthesized compounds might be useful remedies for the treatment of inflammatory diseases by inhibiting the action of IL-6.

Poster Presentation


Poster Presentation

PNB-64

Molecular Target of Berchemia racemosa Siebold & Zucc. for Anti-cancer Activity in Human Colorectal Cancer: Cyclin D1

proteasomal Degradation

Da Som Kim*, Yeongyeong Kang, Hyun Ji Eo, Youngki Park, Gwang Hun Park


In this study, we evaluated the effect of the extracts of branches from Berchemia racemosa Siebold & Zucc. (BRS-B) on cell proliferation and the molecular mechanisms in human cancer cells. The treatment of BRS-B suppressed the cell proliferation in HCT116 and SW480 cells and decreased cellular accumulation of cyclin D1 protein. To evaluate whether BRS induces cyclin D1 proteasomal degradation, the cells were pretreated with MG132 as a proteasome inhibitor and the co-treated with BRS-B. BRS-B decreased cyclin D1 protein level in absence of MG132. However, the presence of MG132 attenuated BRS-induced cyclin D1 downregulation, which indicates that BRS-B-mediated cyclin D1 downregulation may be attributed to cyclin D1 proteasomal degradation. The current study provides new mechanistic link between BRS-B, cyclin D1 downregulation and cell growth in human colorectal cancer cells. In conclusion, BRS-B has a potential candidate for the development of chemopreventive or therapeutic agents for human colorectal cancer.

PNB-61

Validation of Analytical Method and Antioxidant Activities of Eriobotrya japonica Lindl. Leaves Extract according to

Extraction Solvent

Na Young Byun, Hyun Hee Kim, Ji Hye Kim, Jae Seung Han, Seo Wk Lee, Hyang Eun Kim, Eun Ju Kim, Soon Ho Yim*


The antioxidant activities of leaves extract from Eriobotrya japonica were investigated using DPPH and ABTS radical scavenging activity. The extraction yield of the E. japonica leaves and young leaves extracts using 80% ethanol were 22.0% and 23.9%, respectively. The total flavonoid contents of E. japonica leaves extracts were 81.6~2,149.7 mg quercetin equivalent/g in 80% ethanol extract. Also, the 80% ethanol extract of leaves (IC50 values of DPPH and ABTS were 13.9 μg/ml and 10.9 μg/ml) and young leaves (IC50 values of DPPH and ABTS were 20.7 μg/ml and 17.3 μg/ml) showed higher radical scavenging activity than 20% ethanol and water extracts. Furthermore, the quantitative determination method of ellagic acid and chlorogenic acid as antioxidant compounds from E. japonica leaves was optimized by HPLC/DAD method. This method showed high linearity of the calibration curve with a coefficient of correlation (R2) of 0.999, and limit of detection was 2.35 μg/ml (ellagic acid) and 0.73 μg/ml (chlorogenic acid) as well as limit of quantification was 7.13 μg/ml (ellagic acid) and 2.22 μg/ml (chlorogenic acid), respectively. Recovery of ellagic acid and chlorogenic acid was 99.7~108.0% with RSD values less than 5.31%. These results suggest that 80% ethanol extract of E. japonica leaves could be a potential source of natural antioxidant for use in food, cosmetics and pharmaceuticals industries.

PNB-62

Validation for Simultaneous Determination of three marker components in the Eriobotrya japonica extract by HPLC-DAD

Ye-Seul Yong, Hyun-Hee Kim, Woo-Jin Jeon, Min-Ah Kang, In-Hwa Kim, Hee-Eun Chae, Soon-Ho Yim*

Department of Pharmaceutical Engineering, Dongshin University

The simultaneous determination of marker components in the Eriobotrya japonica extract was established using HPLC-DAD. The HPLC method such as SHISEIOD column (UG1230, CAP CELL PAK, 5 μg μm, 4.6×250 mm), 0.1% formic acid in water and acetonitrile solvent was used for the separation of marker components such as the chlorogenic acid, cryptochlorogenic acid and neochlorogenic acid. Verification of marker components was evaluated as specificity, linearity, accuracy, precision and recovery. The correlation coefficient (R²) value of the three marker components was showed excellent linearity (0.999~1). The detection limit (LOD) and the quantitative limit (LOQ) were in the range of 0.112 μg/ml~1.043 μg/ml and 0.245 μg/ml~ 0.741 μg/ml, respectively. Relative standard deviation (RSD) values from intra-day and inter-day precision were less than 0.50%. Recovery rate at 10, 40, 80 μg/ml was in the range of 95.6~102.3% with RSD values less than 1.18%. These results provide an HPLC-DAD simultaneous determination of three marker components in the E. japonica.

PNB-63

Correlation between Antioxidant and Antioxidant Activity in 3029Extraction Conditions of Eriobotrya japonica (Thunb.)

Lindley leaf and flesh

Ho Young Seon, Soon Ho Yim*, Yong Dae Kim, Si Young Jung, Yu Min Choi, Hy Bin Shin


This study was carried out to investigate the polyphenol contents (TPC) and the flavonoid contents (TFC) correlation between antioxidant activity. The variety extraction methods in Eriobotrya japonica (Thunb.) Lindley leaf and flesh were performed distilled water, ethanol (0, 20, 40, 60, 80, 100%) for 4 hours at 100℃ and these extracts were concentrated under reduced pressure, freeze dried, and stored at 4℃. TPC and TFC of E. japonica leaf and flesh extracts showed in range of 2.5~33.26 mg tannic acid equivalent/g and 13.93~96.41 mg catechin equivalent/g, respectively. TPC and TFC were highest in hot water leaf extract (33.26 mg/g and 96.4 mg/g, respectively). Results of antioxidant activity by DPPH and ABTS methods showed that 60% ethanol leaf extract possessed the most potent scavenging capacity by 84% and 96%, respectively. However, the 100% ethanol leaf extract was showed the highest DPPH scavenging rate by 86%. These results suggest that E. japonica leaf extract may be useful as a potential antioxidant than flesh estracts.


214

PNB-68

Phenotypic and genetic analysis of a new mutant related to development of tomato trichome and plant growth

Won-Ki Hong1, Jae-In Chun1, Seong-Min Kim1, Jin-Ho Kang1,2*1GSIAT, Graduate School of International Agricultural Technology and Crop Biotechnology, Pyeongchang 25354, Republic of Korea, 2GBST, Institute/GreenBio Science and Technology,

Seoul National University, Pyeongchang 25354, Republic of Korea

Trichomes are hair-like structure derived from plant epidermis that acts as a plant defense against biotic stresses and abiotic stresses and are uni- or multicellular structures. Trichomes exist in the wide range of plant species and are classified as either glandular or non-glandular types. Glandular trichomes function in a chemical defense or physical defense against herbivore, and non-glandular trichomes function as physiological barriers for biotic and environmental stresses. In this study, we characterized a monogenic recessive tomato mutant called no trichome (nt). To analyze the morphology of trichomes on the nt mutant in detail, we observed trichomes with a dissecting microscope and a scanning electron microscopy (SEM). Compared with wild-type plants which have four types of glandular (type I, IV, VI, VII) and three types of non-glandular (type II, III, V) trichomes on the all aerial tissue, the nt mutant does not have all types of trichomes on young stems. In addition, the nt mutant has shorter stems, fewer branches, and smaller fruits compared with wild-type plants. To identify the NT gene, we are conducting the map-based cloning and narrowed down the mapping region about 2.2 Mb.

PNB-65

Anticancer Activity of Extracts from Different Parts of Berchemia racemosa var. magna in Human Cancer Cells

Yeongyeong Kang*, Gwang Hun Park


The aim of this study, we evaluated the anti-cancer activity of extracts of the Berchemia racemosa var. magna (BR) which belongs to Rhamnaceae against human cancer cells. In Korea, BR is grown only in pine forests in Anmyeon island, Chungcheongnam-do. It is designated as a special forest protection base in accordance and is evaluated as vulnerable species (VU/Vulnerable) at national level. BR has been reported to have antioxidant and whitening effects. However, the anticancer activity of BR have not been elucidated. So, we was performed to elucidate the cells viability by parts of BR extracts in human cancer cells. The parts (leaves, branches and fruits) of BR were extracted with 70% ethanol. The types of human cancer cells for evaluating the anticancer activity were colorectal cancer (HCT116, SW480), breast cancer (MDA-MB-231), prostate cancer (PC-3), lung cancer (A549), liver cancer (HepG2), and pancreatic cancer (AsPC-1). Human cancer cell viability was measured using MTT assay. As a result, inhibition of human cancer cell proliferation was greatly inhibited by fruits of BR. These results suggest that extracts of fruits of BR can be used as a supplementary material for anticancer and as a material for developing natural anticancer drug.

PNB-66

Resveratrol conjugated with gold nanoparticle induces cell cycle arrest in MCF-7 cell line

Dong Gun Lee, Yong Hun Joo, Hyeri Lee, Jimin Lee, Sang Gyu Lee, Na-Hyun Lee, Namhyun Chung*


Breast cancer is one of the leading cause of cancer-associated death in women. Various chemotherapy and radiation exposure have been attempted to treat the cancer but anti-cancer drugs have several side effects on normal cells. To solve the related problems, various natural compounds have been investigated as candidate drug for cancer therapy. Resveratrol is a kind of phytoalexin which is produced for self-defensive effect in several plants. It has been also reported to have anti-cancer effects to cancer cell including breast cancer cell line. To enhance the therapeutic efficacy of resveratrol to cancer cells, we hypothesized that resveratrol need to be linked to gold nanoparticle as a carrier. Gold nanoparticles under 30 nm were synthesized by seed-growth method and conjugated with resveratrol through polyvinylpyrrolidone as a cross-linker (GRs). Cell cycle analysis were conducted to confirm the anti-tumor activity of GRs on MCF-7 cell line. Lactate dehydrogenase (LDH) assay were performed to examine whether or not GRs have a degree of cytotoxicity to Raw264.7 cells. As results, GRs induced S phase arrest compared with control groups. In addition, GRs had almost no cytotoxicity toward Raw264.7 cells. These results suggest that GRs could be applied to the cancer treatment with further development. This work was supported by a grant from National Research Foundation of Korea (Grant No. NRF-2015R1A2A2A0100650).

PNB-67

Rice extracts exhibit positive effects on mitochondria function of ovary removed mouse in tissue specific manner

Da Young Kim1, Min-Young Um2, Soo Im Shin1*1Interdisciplinary Program of Bioenergy and Biomaterials Graduate School, College of

Engineering, Chonnam National University, Gwangju 61186, Republic of Korea, 2Division of Functional Food Research, Korea Food Research Institute 245,

Nongsaengmyeong-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do 55365, Republic of Korea

Women in menopause experience diverse physical and mental changes such as weight gain, sleep disturbance because of reduced estrogen caused by ovarian dysfunction. Administration of estrogen relieve the menopause symptoms, but they have side effects such as breast cancer by long-term administration. In this study, we examined 4 groups of ovary removed mice including normal, high fat, high fat+estradiol, high fat+rice extracts diet to know how rice extracts affect to mitochondrial function in brain and liver because mitochondria is a key organelle playing a vital role in energy metabolism and control various signals and hormones in a cell. The results in brain represent that the high fat+rice extracts diet group increased amount of mitochondrial protein, enzyme activities and expression level of OXPHOS complex I, IV but decreased oxidative stress compared to high fat diet group. These effects were even greater than those in high fat+estradiol diet group. In liver, however, rice extracts did not affect the function of mitochondria at all. It indicates that rice extracts have a positive effect to not liver but brain specifically. Our results suggest that rice extracts improve the function of brain mitochondria, so it might be used as alternative substance to alleviate the symptoms of menopause as a natural product.

Poster Presentation


Poster Presentation

PNB-72

Stability of Oleic acid and Linoleic acid on organic inputs derived from Tea see oil

Jeewon Rhee, Song-Hee Ryu*, Hyoin Yoon, Geun-Hyoung Choi, Hyejin Yu, Hyo-Sub Lee, Sung-Jin Lim, Sang-Won Bark


Tea seed oil is essential oil derived from the seeds of Camelia oleifera. Tea seed oil has been used as raw material of commercial organic inputs in Republic of Korea. It is known that tea seed oil contains abundant of oleic acid and linoleic acid and both compounds have insecticidal effect. Therefore, this study investigated the thermal stability of oleic acid and linoleic acid as active compounds in commercial organic inputs derived from tea seed oil. As a result of evaluating the stability of oleic acid and linoleic acid at 4℃, 25℃, 35℃, 45℃ and 54℃, oleic acid and linoleic acid were very stable in commercial organic inputs. Even when stored at room temperature, the active ingredients were found to be stable for over one year.

PNB-69

Assessment of the aromatase inhibitory activity of Ma-Huang-Tang (MHT) and its active compounds

Dong Ho Jung, Byoung Seob Ko*, Joo Tae Hwang, Bo-Jeong Pyun, Song Yi Yu

Division of Herbal Medicine Research, Korea Institute of Oriental Medicine

In a search for a new herbal medicine that inhibits aromatase (CYP19), a steroidogenesis-inducing enzyme targeted in the treatment of hormone-dependent diseases, a new aromatase inhibitory effect of Ma-Huang-Tang (MHT), a traditional Korean herbal medicine prescription, was scientifically verified. This study used dibenzylfluorescein (DBF) and KGN cells to observe the effects of MHT on aromatase activity and developed a reproducible HPLC verification method for the active components. The results indicate that MHT demonstrates a dose-dependent inhibitory effect on aromatase with an IC50 value of 251 μg/mL. Furthermore, among the six herbal medicines that constitute MHT, Ephedra sinica Stapf, Cinnamomum cassia Presl and Glycyrrhiza uralensis Fischer showed the most potent inhibition of aromatase activity, with IC50 values of 1.465, 1.535 and 1.247 mg/mL, respectively. In addition, three marker compounds, liquiritin, liquiritin apioside and liquiritigenin, of the active herbal medicines showed inhibition of aromatase activity, with IC50 values of 530, 508 μM and 1.611 mM, and their content was determined as 15.58, 19.80 and 2.22 mg/g, respectively, by using HPLC/DAD. MHT and the three marker compounds inhibited estrone production and aromatase activation in KGN cells. These results indicate that the aromatase inhibitory effect of MHT is a synergistic effect of its active components and that MHT has potential as a preventive agent against aromatase activity.

PNB-70

Anti-obesity effect of Standardized Extract of Microalga Phaeodactylum tricornutum Containing Fucoxanthin

Songyi Koo1, Jae-In Um2, Sang Min Kim3*1Natural Product Informatics Center, KIST Gangneung Institute of Natural Products,

2AlgaeTech, 3Smart Farm Research Center, KIST Gangneung Institute of Natural Products

Fucoxanthin (FX), a marine carotenoid found in macroalgae and microalgae, exhibits several beneficial effects on health. The anti-obesity activity of FX is well documented, but FX has not been mass-produced or applied extensively in commercially because of limited availability of raw materials and complex extraction techniques. In this study, we investigated the anti-obesity effect of standardized FX powder (Phaeodactylum extract, PE) developed from microalga Phaeodactylum tricornutum as a commercial functional food. The effects of PE on adipogenesis inhibition in 3T3-L1 adipocytes and anti-obesity in high-fat diet (HFD)-fed C57BL/6J mice were evaluated. PE and FX dose-dependently decreased intracellular lipid contents in adipocytes without cytotoxicity. In HFD-fed obese mice, PE supplementation for 6 weeks decreased body weight, organ weight, and adipocyte size. In the serum parameter analysis, the PE-treated groups showed attenuation of lipid metabolism dysfunction and liver damage induced by HFD. In the liver, UCP1 upregulation and PPARγ downregulation were detected in the PE-treated groups. Additionally, micro computed tomography revealed lower fat accumulation in PE-treated groups compared to that in the HFD group. These results indicate that PE exerts anti-obesity effects by inhibiting adipocytic lipogenesis, inducing fat mass reduction, and decreasing intracellular lipid content, adipocyte size, and adipose weight.

PNB-71

Aldose Reductase Inhibition of the Methanolic Extracts of Exotic Plants

Ju Sung Lee1, Dong Geon Woo2, Ga Eul Cho2, Dong-Gu Lee1, Sanghyun Lee1*1Department of Plant Science and Technology, Chung-Ang University, Anseong 17546,

Korea, 2Sejong Academy of Science and Arts, Sejong 30099, Korea

The inhibition of aldose reductase (AR) by the extracts from exotic plant species in Korea was estimated. Among them, Ambrosia trifida showed the highest inhibition of AR. The content of quercetin, a potent aldose reductase inhibitory flavonoid, in the samples was then analyzed using high performance liquid chromatography coupled with UV detector. Based on the results, Ambrosia trifida had not detectable quercetin.


216

PNB-76

Exploring Step-by-Step Assembly of Nanoparticle: Cytochrome Biohybrid Photoanodes

Ee Taek Hwang*


Coupling light-harvesting semiconducting nanoparticles (NPs) with redox enzymes has been shown to create artificial photosynthetic systems that hold promise for the synthesis of solar fuels. High quantum yields require efficient electron transfer from the nanoparticle to the redox protein, a property that can be difficult to control. Here, we have compared binding and electron transfer between dye-sensitized TiO2 nanocrystals or CdS quantum dots and two decaheme cytochromes on photoanodes. The effect of NP surface chemistry was assessed by preparing NPs capped with amine or carboxylic acid functionalities. For the TiO2 nanocrystals, binding to the cytochromes was optimal when capped with a carboxylic acid ligand, whereas for the CdS QDs, better adhesion was observed for amine capped ligand shells. When using TiO2 nanocrystals, dyesensitized with a phosphonated bipyridine Ru(II) dye, photocurrents are observed that are dependent on the redox state of the decaheme, confirming that electrons are transferred from the TiO2 nanocrystals to the surface via the decaheme conduit. In contrast, when CdS NPs are used, photocurrents are not dependent on the redox state of the decaheme, consistent with a model in which electron transfer from CdS to the photoanode bypasses the decaheme protein. These results illustrate that although the organic shell of NPs nanoparticles crucially affects coupling with proteinaceous material, the coupling can be difficult to predict or engineer photosystems.

PNB-73

Deposition of iron-based inorganics during Fenton-based lignin humifications and its effects on plant stimulations

Ho Young Yoon, Hae Jin Jeong, Jong-Rok Jeon*

Department of Agricultural Chemistry and Food Science & Technology, Gyeongsang National University

Since commercial humic acids widely used for agronomical purposes contain a significant amount of iron species, it is of interest to introduce such inorganics during artificial lignin humification pathways. Fenton-based oxidations are known to result in deposition of Fe-related inorganics as well as production of reactive oxygen species. Here we report that Fenton treatments on technical lignins induce efficiently the deposition of iron-based inorganics in the lignin variants structurally modified via the Fenton ROS. Detailed content of the deposited iron-based inorganics was compared with that of commercial humic acids by employing ICP-OES. To test whether the presence of the iron-based inorganics in the lignin variants affects plant stimulations, acid washing was employed to fully remove the Fe-related inorganics from the lignin variants. The Fe contents revealed by ICP-OES suggest that most Fe inorganics were removed after the washing. The extent of seed germination of Arabidopsis thaliana treated with Fe-containing lignin variants was compared with that of Fe-washed ones. The similar germination rates were observed whether the iron species were present or absent, suggesting that the short-term effects of Fenton-based lignin variants on plant stimulations could be maintained without the iron species. However, their long-term effects on plant growth & development should be further monitored to decipher the exact roles of irons present in commercial humic acids for plant stimulations.

PNB-74

Highly Stable and Fine-Textured Hybrid Microspheres for Entrapment of Cosmetic Active Ingredients

Ee Taek Hwang*


This study details the preparation and application of supramolecular host-guest inclusion complexes entrapping biomineralized microspheres for long-term storage and their pH-responsive behavior. The microspheres were assembled using a CaCO3 synthesis process coupled with tetrahydrocurcumin-cyclodextrin (THC-CD) inclusion complexes, forming fine-textured and mechanically stable hybrid materials. The products were successfully characterized using SEM, EDS, FT-IR, XRD, and PSA. Various parameters such as the Brunauer– Emmett– Teller (BET) surface area, single point total pore volume, and pore size via adsorption/desorption analysis were also determined. The obtained THC-entrapped hybrid microspheres contained as high as 20% THC loading, and were very stable, preserving 90% of the initial concentration over four weeks of storage at different temperatures, largely limiting THC leaching, and indicating high stability in a physiological environment. In addition, the pH-responsive release of THC from the hybrid microspheres was observed, showing potential uses for application to the weakly acidic skin surface. To our knowledge, this is the first demonstration of anti-aging cosmetic formulation technology using biomineralization based on the co-synthesis of CaCO3 and CD-THC complexes.

PNB-75

Protein Inserted into Vesicle for Artificial Liposome Preparation

Ee Taek Hwang*


There is a growing interest in the preparation of lipid vesicles able to encapsulate labile biological substances such as proteins, peptides or nucleic acids for the purposes of pharmaceutical, cosmetic and chemical applications. Here, we aimed to design this by coupling membrane bound protein with hydrophilic small molecule by reconstitution and sequential rapid dilution. The MtrCAB membrane bound protein inserted within the bilayers of liposomes that have encapsulated small molecule reactive red 120 in order to exploit the electron transfer through MtrCAB in a compartmentalised fashion. The encapsulated molecule reduction via MtrCAB protein is confirmed by absorbance spectroscopy and spectroeletrochemistry. In other words, in these hybrid systems, creating artificial photosynthetic system in which a redox protein is used to mimic the efficient charge separation found in biological photosystems. Furthermore, the small molecule encapsulated proteoliposome platform inspire a new perspective for investigations in various cosmetic, biomedical application.

Poster Presentation


Poster Presentation

PNB-80

Isolation and Identification of Isoflavones and Soyasaponins in Germs of Korean Soybean (Glycine max L.) Cultivars by

UHPLC-Orbitrap-MS

Kwang-Sik Lee1,2, Hyeon-Mi Ham1, Hyun-Young Kim1, Mi-Ja Lee1, Ki-Chang Jang1, Hye-Lim Shin1, Ji-Eun Ra1, Woo-Duck Seo1*

1Division of Crop Foundation, National Institute of Crop Science, 2Department of Crop Science and Biotechnology, Dankook University

Soybean is widely used in healthy foods and nutritional support. Especially, soybean germ (SG) has received much attention because of their abundance of nutritional and biological components. This study aimed to isolate and identify isoflavones (IS) and soyasaponins (SS) in germs of twenty four Korean soybean cultivars by multiple preparative liquid chromatography (MPLC) and ultra-high performance liquid chromatography coupled to Orbitrap mass spectrometer (UHPLC-Orbitrap-MS), respectively. Twenty four compounds were identified as twelve IS and fourteen SS in methanol extracts of SGs. The identified IS were confirmed in germ of all soybean cultivars used this study. However, among the identified SS, SS Aa, SS Au and SS Ae were only detected in three cultivars (Socheongja, Taeseon and Taekwang). Also, SS Ac and SS Af were only detected in twenty one cultivars. These isolated SS data were subjected to principal component analysis (PCA) to examine whether separate SS phenotypes of cultivars. PCA revealed two clusters corresponding to the Aa type by Ab type. A total of 78.9% of the variation among cultivars was explained by principle component 1 (PC1, 47.9%) and principle component 2 (PC2, 31.0%). In conclusion, our results suggest that evaluation of SGs could be beneficial clues for developing soy-mediated functional food materials.

PNB-77

UPLC-QTOF/MS and HR-MAS NMR-based metabolomics applied for the quality evaluation of four processed Panax

ginseng products

Bo-Ram Choi, Dahye Yoon, Jae-Suk Ban, Ryong Gong, Young-Seob Lee, Geum-Soog Kim, Dae Young Lee*

Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, Republic of Korea

The root of Panax ginseng C.A. Meyer has been widely used as ingredients for traditional medicines and functional foods. Furthermore, not only raw ginseng but also several processed ginseng products have been used in the food industry and herbal markets. According to the distinct processing methods, there are four processed P. ginseng products including white ginseng (WG), tae-geuk ginseng (TG), red ginseng (RG), and black ginseng (BG).In this study, HR-MAS NMR and UPLC-QTOF/MS-based metabolomics approaches were applied to assess the metabolic compositions of four processed ginseng. In the results of HR-MAS NMR, primary metabolites were identified such as sugars and amino acids. In the multivariate analyses of NMR spectra, PCA score plot showed that TG and RG were clustered each other, and discriminated from WG and BG. The main metabolites causing these cluster were sucrose and maltose. Maltose and sucrose were not detected in the WG and BG, respectively. In PCA score plot, four groups of processed ginseng were well differentiated. OPLS-DA was also performed to discriminate two selected samples. Several ginsenosides were found as the key components to differentiate four ginseng products. UPLC-QTOF/MS with an in-house library was used to profile ginsenosides from these four ginseng products. The numbers of identified ginsenosides from the four products were as follows: WG (n=26), TG (n=28), RG (n=34), and BG (n=36).

PNB-78

Identification and quantitative analysis of metabolites from Scrophularia buergeriana and their hepatoprotective effects

against HepG2 Cells

Seon Min Oh1,2, Hyeon Seon Na1, Jeon Hwang-bo2, Dahye Yoon1, Young-Seob Lee1, Geum-Soog Kim1, Dae Young Lee1*

1Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, 2Graduate School of Biotechnology and Department of Oriental Medicine

Biotechnology, Kyung Hee University

Scrophularia buergeriana Miquel belongs to Scrophulariaceae is a plant which native to Northeast Asia. S. buergeriana is perennial herb that plays a significant role in traditional medicines. The root of S. buergeriana has been used to treat diseases such as fever, swelling, constipation, pharyngitis, neuritis and laryngitis. S. buergeriana contains various constituents such as iridoid glycoside, phenylethanoids, terpenoids, flavonoids, and sterol. Phenylethanoid and iridoid glycoside are the main constituents of S. buergeriana. Phenylethanoid displayed neuroprotection in primary cultures of rat cortical cells injured with glutamate and hepatoprotective effect on CCl₄ induced toxicity. Also, iridoid glycoside has been effect on protecting scopolamine induced neurotoxicity in vitro and in vivo. This study was carried out identification of metabolites and quantify of major compounds using HPLC from S. buergeriana extracts. NMR techniques and quantitative analysis can provide scientific basis for the analytical studies of S. buergeriana. Additionally, the major compounds isolated from S. buergeriana for its in vitro hepatoprotective activity against ethanol in HepG2 cell lines.

PNB-79

Identification of Glycosylated Flavonoids and Steroidal Saponins from Oat (Avena sativa. L) Seedlings and Their

Osteoclast Differentiation Inhibition

Hye-Lim Shin1,2, Ki-Chang Jang1, Mi-Ja Lee1, Hyun-Young Kim1, Ji-Eun Ra1, Hyeon-Mi Ham1, Woo-Duck Seo1*

1Division of Crop Foundation, National Institute of Crop Science, 2Department of Biological Sciences, Chonbuk National University

This study aimed to identify of glycosylated flavonoids and steroidal saponins from oat seedlings (OS). Four compounds were isolated from the extracts of OS by an octadecyl silica gel (ODS) column chromatography and a multiple preparative Liquid chromatography (MPLC) purification system. Their chemical structures were confirmed as isovitexin 2-O″-arabinoside (OS-0), 3-O-feruloylquinic acid (OS-3), avenacoside A (OS-7), avenacoside B (OS-8) by nuclear magnetic resonance (NMR) and Ultra-high performance liquid chromatography coupled to Orbitrap mass spectrometry (UHPLC-Orbitrap-MS). The oat seedlings extract (OSE) dose-dependently inhibited RANKL-induced osteoclast differentiation with attenuation of ERK phosphorylation, c-Fos and NFATc1 molecules in osteoclastogenesis. In the cell migration and maturation of osteoclastogenesis, OSE also prevented DC-STAMP and cathepsin K, which are required for cell fusion and bone resorption, such as osteoclast function. In conclusion, this study indicated that OS may be useful for functional food materials and prevention or treatment of osteoclast-mediated bone metabolic diseases, including osteoporosis, rheumatoid arthritis.


218

PNB-84

Lutonarin from barley seedlings inhibits inflammation through suppressing of Nuclear Factor-kB signaling pathway on

LPS-stimulated RAW 264.7 macrophage cells

Woo Duck Seo*, Mi-Ja Lee, Ji Eun Ra, Hyun Young Kim, Hyeon Mi Ham, Ki Chang Jang

National Institute of Crop Science, Rural Development Administration (RDA)

Lutonarin (LN), natural plant flavonoids, has an antioxidant activity and the major component of barley seedlings (BS). LN isolated from BS were identified by ultra-performance liquid chromatography-quadrupole-time-of-flight-tandem mass spectrometry (UPLC-Q-TOF-MS/MS). It inhibited the overexpression of nuclear factor-kB. NF-kB has an important role in the immune system and tissue stability; however, excessive activation of NF-kB causes inflammation. DNA binding, p65 phosphorylation and nuclear translocation, IkBa phosphorylation, and NF-kB reporter gene expression decreased in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages treated with LN, whereas IkBa degradation increased. Furthermore, LN suppressed pro-inflammatory cytokine production, including that of interleukin (IL)-6 and tumor necrosis factor (TNF)-a, and inflammatory enzymes such as cyclooxygenase-2 (COX-2). These results indicate that LN has anti-inflammatory effects in LPS-induced RAW264.7 macrophages via suppression of the NF-kB pathway.

PNB-81

Anti-inflammatory flavonoids from root bark of Broussonetia papyrifera in LPS-stimulated Raw264.7 cells

Mi Hyeon Park, Hyung Won Ryu, Doo-Young Kim, Yang Hee Jo, Sei-Ryang Oh*


Broussonetia papyrifera (L.) L’Her. ex Vent. belonging to Moraceae has been used to treat diuretic, tonic and suppressant for edema as traditional medicine. In this study, bioactivity-guided fractionation and metabolites investigation of root bark extracts of B. papyrifera results in the isolation and identification of six 1,3-diphenylpropanes (1, 2, 8, 10, 17, and 20), flavanones (3), two chalcones (4 and 5), five flavans (6, 11, and 14-16), dihydroflavonol (7) and five flavonols (9, 12, 13, 18, and 19) including five new compounds (5, 7, 8, 19, and 20) on inhibits NO production in LPS-induced Raw264.7 cells. The structures of isolated compounds 1-20 were elucidated on the basis of spectroscopic data (1D and 2D NMR, MS, MS/MS, and HRMS). In particular, the highest efficacy for the inhibition of NO, iNOS, and pro-inflammatory cytokine (TNF-alpha and IL-6) production was found in isolated compounds 3, 5, 7, 12, and 20, among which new compounds (broussochalcone C 5, broussoflavonol H 7, and kazinol W 20). Therefore, this study suggests that flavonoids-rich products of B. papyrifera including new compounds can be an excellent candidate for development of novel pharmaceuticals and functional foods agents in the prevention and treatment of anti-inflammation disease.

PNB-82

Flavonoids from stem of Maackia amurensis

Won Jun Kim, Seong Mi Lee, Hyun-Jae Jang, Hyung Won Ryu, Sei-Ryang Oh*


Maackia amurensis (Leguminosae) widely distributed widely in Korea, and Far East Asia. Traditionally, this plant is used as a folk medicine for the treatment of cancer, cholecystitis, and arthritis. In previous study, piceatannol and resveratrol (both stilbenes), formononetin, genistein, retuzin and afromozin (all isoflavones), maackiasine (isoflavonostilbene), scirpusin A, scirpusin B and maackin (all dimeric stilbenes), and maackoline (stilbenolignan) were separated in M. amurensis wood. These compounds have been reported for anti-inflammatory, insecticidal and hepatoprotective activities. In this study, fifteen flavonoids were isolated in the bark of M. amurensis. These compounds were extracted with 100% MeOH, and isolated by activity-guided separation. The structure of isolated compounds were identified by spectroscopic analysis including UPLC-QTof-MS, 1D (1H and 13C-NMR, DEPT), and 2D NMR (COSY, HSQC, HMBC).

PNB-83

Metabolic Profiling Combined with Chemometrics Provides Prediction Models of Antioxidant Properties in Nine

Mentha Species

Seung-A Baek1, Yun Ji Park2, Yongsoo Choi3, Sang Un Park2, Jae Kwang Kim1*1Division of Life Sciences and Convergence Research Center for Insect Vectors, Incheon

National University, 2Department of Crop Science, Chungnam National University, 3Systems Biotechnology Research Center, Korea Institute of Science and Technology (KIST)

Mentha species are well recognized for their medicinal and aromatic properties. The comprehensive metabolite profiles of nine Mentha species have been determined. The extracts of these Mentha species were also screened for antioxidant and free radical scavenging activities. Forty-seven hydrophilic and seventeen lipophilic compounds were identified and quantified from the selected Mentha species. Also, eleven phenolic compounds, riboflavin and eight carotenoids were present, and their composition and content varied among the various Mentha species. The different Mentha species exhibited a range of antioxidant potencies. Horse mint especially exhibited the strongest antioxidant capacities (1,1-diphenyl-2-picryl-hydrazyl (DPPH), hydrogen peroxide, and reducing power assay) among the nine Mentha species. A difference between different samples from the same species was not observed by multivariate analysis. A high correlation between metabolites involved in closely linked biosynthetic pathways has been indicated. The projection to latent structure method, using the partial least squares (PLS) method, was applied to predict antioxidant capacities based on the metabolite profiles of Mentha leaves. According to the PLS analysis, several carotenoid contents, such as E-β-carotene, 9Z- β-carotene, 13Z- β-carotene and lutein, as well as phenolic compounds, showed a positive relationship in reducing the power of Mentha extracts. Horse mint is a good candidate because of its high antioxidant efficacy among the nine Mentha species included in the study.

Poster Presentation


Poster Presentation

PNB-88

Antimicrobial Photodynamic Treatment for Gram Negative Bacteria with Hypericin

Seemi Tasnim Alam1,2, Kyungsu Kang1,2*1Natural Products Informatics Research Center, KIST Gangneung Institute of Natural

Products, 2Division of Bio-Medical Science & Technology, Korea University of Science and Technology (UST)

Pseudomonas aeruginosa, is an opportunistic gram negative pathogen commonly found in environment and hospital acquired infections. This bacterium is found to be resistant to wide range of frequently used antibiotics. Antimicrobial photodynamic treatment (APDT) is often used to kill bacteria with several photosensitizers (PSs) and light treatment. It is already reported that gram positive bacteria are easier to kill by APDT than gram negative bacteria due to their cell wall structure. Previously, we showed that gram positive bacteria, Staphylococcus aureus KCTC 3881 was killed successfully by APDT with hypericin and orange light. In the present study, we tried to kill gram negative bacteria, P. aeruginosa KCTC 1637 by using APDT. We found that hypericin were not up-taken to P. aeruginosa. To overcome this, we used ampicillin 100 µg/ml to damage the cell wall, and checked that ampicillin alone did not kill P. aeruginosa. Ampicillin increase the cell permeability which enhances hypericin uptake to the bacterial cell, and exposed to orange light (590 nm, intensity 150 ±20 W/m2) 3 hours to create enough reactive oxygen species to kill the bacteria. We found significant reduction using this hypericin and ampicillin combination with orange light. We suggest here that damaging the cell wall, inhibiting the efflux mechanism can increase the APDT efficiency by using hypericin in gram negative bacteria P. aeruginosa.

PNB-85

Characterization of policosanols and quality changes related to the growth times from the seedlings of various Korean wheat cultivars and screening for their AMPK activation

Woo Duck Seo, Ji Eun Ra, Hye Lim Shin, Kwang Sik Lee, Ki Chang Jang*, Mi Ja Lee, Hyun Young Kim, Hyeon Mi Ham

National Institute of Crop Science, Rural Development Administration

The main objective of this research was to compare and determine a policosanol profile and AMPK activation in growth times from various Korean wheat seedlings. Nine policosanols differed remarkably in seventeen cultivars through five growth times using GC-MS technique, especially, octacosanol (8) showed the most predominant component (39-83%) with significant variations as average contents of 361.4→613.0→203.0→196.5→50.9 mg/100 g in 3→19 days. The highest average policosanols exhibited 738.7 mg/100 g on 6 days, while the lowest was 104.4 mg/100 g on 19 days. Moreover, the wheat cultivars including Shinmichal 1, Anbaek, Namhae, and Joah on 6 days growth may be recommended as potential sources because of high policosanols (921.7-990.6 mg/100 g). Interestingly, the AMPK activation markedly increased with considerable fluctuations (control; 100%→hexane extracts; 150-370% at 100 g/ml) in this species and the main composition, octacosanol (8) possessed potent AMPK activator (control; 100%→280% at 200 g/ml). It is confirmed that the AMPK activation of wheat seedlings are positively correlated with policosanols, with the samples on 6 days exhibiting the optimal harvest times. Therefore, our results may be attributed to enhance the wheat value in nutrition and biological properties.

PNB-86

Air Drying Temperature effects on Physiological and Effective Components of Thuja orientalis L.

Kyeong Yeong Song1, Jin Hwan Seo1, Youngju Kim2, Kyung Dong Lee1*1Oriental Medicine Materials, Dongshin University, Naju, Jeonnam 520-714, Korea,

2Ireh Hi-Tech Inc., Eunha-gil 93, Suncheon 540-742, Korea

Thuja orientalis L. is important medicinal plant that has been historically used for hematemesis, antihypertensive drugs and health food. Recently, it is known for its excellent anti-bacterial, anti-inflammatory and antioxidant activity, but there is not enough to meet physiological activity by air drying temperature in leaves of T. Orientalis L. (room tem., 45, 60 and 80℃). Our results indicated that the physiological activity on the methanol extract was decreased significantly with increasing air-drying temperature levels. The total polyphenol and flavonoid content at 80℃ treatments were significantly lower 67 mg/g and 3.3 mg/g, compared with control (room temperature) treatment 75 mg/g and 4.0 mg/g, respectively, DPPH scavenging ability was the highest at 92.9% at room temperature. The antimicrobial effect of methanol extracts against air drying temperature changes was highest in Vibrio vulnificus. These results suggest that the extract could be used in functional foods.

PNB-87

Physiological Activity Changes of Eriobotrya japonica Extracts with Air Drying Temperature Levels

Kyeong Yeong Song1, Jin Hwan Seo1, Youngju Kim2, Kyung Dong Lee1*1Oriental Medicine Materials, Dongshin University, Naju, Jeonnam 520-714, Korea,

2Ireh Hi-Tech Inc., Eunha-gil 93, Suncheon 540-742, Korea

Eriobotrya japonica as medicinal and functional materials are widely grown in coastal areas of Jeonnam province, where the climatic conditions are suitable for their growth. E. japonica is known for its anti-bacterial, anti-inflammatory and antioxidant activity, but there is not enough to meet physiological activity by air drying temperature in their leaves (room tem., 45, 60 and 80℃). This study was carried out by methanol extracts to determine the content and biological activities of active substances contained in the leaves of E. japonica. The total polyphenolic compound content and antioxidant activities of ethyl acetate fraction were found to be 5.0 mg/g and 88 mg/g, respectively, and investigated the highest content than other fractions. The total polyphenol and flavonoid content at 80℃ treatments were significantly lower 38 mg/g and 3.8 mg/g, compared with control (room temperature) treatment 42 mg/g and 1.3 mg/g, respectively. DPPH scavenging ability was the highest at 55 mg/g at room temperature. The antimicrobial effect of methanol extracts against air drying temperature changes was highest in Vibrio vulnificus. This experiment suggests that bioactive substance components affected by highly air drying temperature could decrease the quality of E. japonica.


220

PNB-92

Expectation of Unknown Secondary Metabolites of Carex bostrichostigma maxim using UPLC-Q-TOF/MS Analysis

Bongkyu Song1, Dae-Choul Kim2, Qingzhen Liu2, Hye Yoon Park3, Ga Ryun Kim3, Wan Teak Im1,2*

1Ginsenoside Research Team, AceEMzyme Co., Ltd., 2Department of Biotechnology, Hankyong National University, 3Biological and Genetic Resources Assessment Division,

National Institute of Biological Resources

Carex boostrichostigma Maxim is an indigenous plant that grows in its natural state without human protection in South Korea. Carex boostrichostigma maxim is hard for harvesting in large quantities because it grows in a natural state unlike plant-breeding, and there is a lack of reporting on component analysis. We explored metabolites for similar species primarily through PCIBD and paper and analyzed metabolites through UPLC-Q-TOF/MS analysis in order to analyze components for Carex bostonigma Maxim. When the literature survey on similar species and the UPLC-Q-TOF/MS database were analysed successfully, 20 metabolites were identified that were not found through the solutions.

PNB-89

Longifolioside A inhibits inflammatory responses through blockade of NF-κB and AP-1 in lipopolysaccharide-stimulated

THP-1 macrophages

Eun Sol Oh1,2, Su Ui Lee1, Hyung Won Ryu1, Hyunju Ro2, Sung Tae Hong3, Sei Ryang Oh1*

1Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Republic of Korea, 2Department of Biological Sciences, College of

Bioscience and Biotechnology, Chungnam National University, Daejeon, Republic of Korea, 3Department of Anatomy & Cell Biology, Department of Medical Science, College of

Medicine, Chungnam National University, Daejeon, Republic of Korea

Pseudolysimachion rotundum var. subintegrum has been used as a traditional herbal remedy for the treatment of respiratory inflammatory disease. Longifolioside A isolated from Pseudolysimachion rotundum var. subintegrum is an iridoid glucoside compound. In previous study, longifolioside A exert a potent anti-oxidant activity. However the underlying pharmacological mechanism of longifolioside A in inflammatory diseases is still unknown.We found that longifolioside A significantly reduced the expression levels of inflammatory cytokine genes such as interleukin (IL)-6, -8, and tumor necrosis factor (TNF)-α in the LPS-stimulated THP-1 macrophages. In addition, longifolioside A inhibited the expression of inflammatory mediator genes such as inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 that are key enzymes generating nitric oxide (NO) and prostaglandin E2 (PGE2), respectively. Longifolioside A suppressed the phosphorylation of IRAK4, IKKα/β, IκBα and mitogen-activated protein (MAP) kinases (ERK 1/2 and JNK, but not p38), thereby inactivating NF-κB and AP-1 to decrease the expression level of inflammatory response genes.There results suggest that longifolioside A attenuates the activation of LPS/TLR4 signal transduction pathway downstream effectors, thereby suppression of NF-κB and AP-1 activation.

PNB-90

The effects of extracts of Bok choy (Brassica rapa subsp. chinesis) on transcriptome sequencing in

Drosophila melanogaster

Soo Yeon Cho, Hyeon Jung Kim, Sung Hyun Lee, Kyung A Hwang, Jeong Sook Choe, Hwan Hee Jang*

Functional Food Division, National Institute of Agricultural Sciences, Rural Development Administration

Bok choy (Brassica rapa subsp. chinensis) was originally cultivated in china and distinct from another leafy vegetable. It is widely cultivated and consumed in china and north eastern asia and is increasingly grown in northern Europe and north America. Although brassica vegetables such as cabbage, broccoli, and kale are known to have physiological effects, few studies have focused on the biological activity of bok choy. In the present study, differentially expressed genes were obtained by analyzing the transcriptome sequencing of drosophila melanogaster fed with bok choy extracts for five days. Oxidative Phosphorylation, Endocytosis, protein processing in Endoplasmic reticulum, longevity regulating pathway were obtained significant value. Especially, significant differences were confirmed in heat shock protein (HSP), lethal (2) essential for life (l(2)efl), NADH-ubiquinone oxidoreductase chain 2 (ND2).Bok choy extract showed up-regulation of HSP70 and heat shock factor (HSF) genes in fruit fly. HSP70 gene plays a role in helping cells to function normally. HSP70 is also found in the human and is known to protect neurons by expression in various diseases such as Parkinson’s disease and Alzheimer’s disease. These results indicate that Bok choy extract may regulate the expression of HSP 70 in mammals including humans.

PNB-91

Antimicrobial and Anti-inflammatory Activity of the Extracts and Fraction from Boehmeria nivea var. nipononivea

Ji Young Kim*, Gi Soo Jung, Sun Hee Lee, Soo-Kyung Yang, Gwanpil Song, Li Yeon Kim

Jeju Biological Resource, Jeju Biological Resource Co., Ltd., Jeju 63242, Korea

This study was designed to investigate the possible utilization of Boehmeria nivea var. nipononivea extract for the development of natural cosmetics ingredients. The leaves of B. nivea var. nipononivea, extracted by 80% ethanol, were sequentially fractionated with n-hexane, dichloromethane, ethylacetate, and n-butanol. Antimicrobial activities of Staphylococcus aureus, Staphylococcus epidermidis and Propionibacterium acnes strains including two antibiotic resistant strains were observed in paper disc method and MIC showed inhibition of bacterial growth. In antibacterial activity by the disc diffusion assay against S. aureus, S. epidermidis and P. acnes, the dichloromethane and ethylacetate fraction showed stronger antibacterial activity than other fractions and the extract. Moreover, the ethyl acetate fraction showed strong nitric oxide (NO) production inhibitory effect in lipopolysaccharide (LPS)-stimulated RAW 264.7 cell. However, in NO scavenging ability, the chloroform fraction was higher than the other fractions and the extract. In conclusion we found that B. nivea var. nipononivea extracts is not cytotoxic and has antimicrobial and anti-inflammatory effects. Therefore, it is suitable for use as a cosmetic material.

Poster Presentation


Poster Presentation

PNB-96

Antibacterial Effect of Conjugated Linoleic Acid on Anaerobic Microorganisms

Sook Jahr Park, Eesul An, Jong Rok Lee*


Conjugated linoleic acid (CLA) is a mixture of different types of isomers of linoleic acid, which is found mostly in meat and dairy products. CLA has been beneficial in lowering body fat and reducing tumors. In the present study, antibacterial effect of CLA were evaluated in against two anaerobic microorganisms. Anaerobic bacteria were cultured in an anaerobic jar with a CO2 gas generator pack. CLA showed strong antibacterial activity against Streptococcus mutans (S. mutans) and Propionibacterium acnes (P. acnes). At the highest amount of CLA tested (100 μg/disk), the mean diameter of the clear zone was recorded at 17 mm for S. mutans and 16 mm for P. acnes. The MIC of CLA was the same as 62.5 μg/ml against both S. mutans and P. acnes. The results of the present study demonstrate that CLA has antibacterial activity against human pathogenic bacterial strains causing dental caries and acne, and indicate possible use of CLA as antibacterial agents.

PNB-93

Expectation of Unknown Secondary Metabolites of Rosa wichuraiana Crép. ex Déségl. Crep. ex Franch. & Sav using

UPLC-Q-TOF/MS Analysis

Bong Kyu Song1, Dae-Choul Kim2, Qingzhen Liu2, Hye Yoon Park3, Ga Ryun Kim3, Wan Teak Im1,2*

1Ginsenoside Research Team, AceEMzyme Co., Ltd., 2Department of Biotechnology, Hankyong National University, 3Biological and Genetic Resources Assessment Division,

National Institute of Biological Resources

Rosa wichuraiana Crép. ex Déségl. Crep. ex Franch. & Sav is an indigenous plant that grows in its natural state without human protection in South Korea. Rosa wichuraiana Crép. ex Déségl. Crep. ex Franch. & Sav is hard for harvesting in large quantities because it grows in a natural state unlike plant-breeding, and there is a lack of reporting on component analysis. We explored metabolites for similar species primarily through PCIBD and paper and analyzed metabolites through UPLC-Q-TOF/MS analysis in order to analyze components for Rosa wichuraiana Crép. ex Déségl. Crep. ex Franch. & Sav. When the literature survey on similar species and the UPLC-Q-TOF/MS database were analysed successfully, 25 metabolites were identified that were not found through the solutions.

PNB-94

Anti-Inflammatory Effect of Broccoli Leaves in LPS-Activated Macrophage Cells

Sook Jahr Park, Eesul An, Jong Rok Lee*


Broccoli is edible green plant that has a wide variety of health benefits, including cancer prevention and cholesterol reduction. However, leaves of broccoli are not eaten and are mostly left as waste. This study was conducted to evaluate the anti-inflammatory effects of the ethanol extract of broccoli leaves (BLE). Cell viability was determined by MTT assay, and NO was determined by reacting cultured medium with Griess reagent. Pro-inflammatory cytokines were detected by ELISA. NO production was dramatically augmented by LPS compared to control. However, the increased NO was dose-dependently attenuated by treatment with BLE. The inhibition of NO production by BLE were due to the suppression of iNOS expression mediated from the inhibition of NF-κB nuclear translocation and Iκ-Bα phosphorylation, determined by western blot analysis. In addition, BLE inhibited the production of inflammatory cytokines such as IL-6 and TNF-α. These results demonstrate that BLE exerts anti-inflammatory activities and also suggest that the waste broccoli leaves could be used for controlling inflammation.

PNB-95

Production of the Rare Ginsenosides Gypenoside LXXV, through the Combinative Use of Two Recombination Enzymes

Dae-Choul Kim1, Bongkyu Song2, Hye Yoon Park3, Youn Kyoung Son3, Sang Yong Kim4, Ji-Hyang Wee4, Wan Teak Im1,2*

1Department of Biotechnology, Hankyong National University, 2Ginsenoside Research Team, AceEMzyme Co., Ltd., 3Biological and Genetic Resources Assessment Division,

National Institute of Biological Resources, 4Department of Food Science & Bio Technology, Shinansan University

Minor ginsenoside, Gypenoside LXXV exists as low content in wild ginseng or red ginseng, but have higher potential pharmacological activities. The purpose of this study was to develop a transformation method which converts major ginsenosides Rb1 into minor ginsenosides above mentioned using two recombinant β-glucosidases (Bgl A and Bgl B) expressed in E. coli. The transformation pathways of major ginsenosides using Bgl A followed by Bgl B were as follows: Rb1 → Gypenoside XVII → Gypenoside LXXV. All the reactions time for the transformation was completed within 1 day at pH 7.0 and 37°C. Gypenoside LXXV was purified by Prep-HPLC column packed with ODS resin followed by using column packed with Silica-gel. As a result, 10 g of Gypenoside LXXV was produced from 100 g of protopanaxadiol type ginsenosides mixture (PPDGM). The purity of this was above 98% chromatographic purity. These results suggest that this enzymatic method would be useful in the preparation of deglycosylated rare ginsenosides for searching the function of those in cosmetic and pharmaceutical industries.


222

PNB-100

Costunolide ameliorates gastric acid reflux-induced esophageal injury via regulating NF-κB signaling pathway



The incidence rate of reflux esophagitis (RE) is gradually increasing worldwide and imposes a great burden on patients in daily life and economic status. Costunolide, a sesquiterpene lactone, is mainly extracted from plants of the families Saussurea and Magnoliaceae. In this study, the effects of anti-inflammation and the protective effects of costunolide against esophageal tissue damage caused by reflux were evaluated in vitro and in vivo, respectively. The anti-inflammatory effects of costunolide were conducted in vitro using LPS-induced RAW 264.7 cell inflammation model. The ameliorative effects of costunolide were confirmed in vivo on reflux esophagitis controlled SD rats model. The results indicated that costunolide inhibited cellular inflammation via regulating NF-κB signaling pathway. In addition, gastric acid reflux led to increased expression of inflammatory proteins including cyclooxygenase (COX)-2, interleukin (IL)-1β and tumor necrosis factor (TNF)-α in esophageal tissues, while costunolide treatment significantly inhibited the expression of the three proteins via regulating activation of NF-κB. Furthermore, costunolide significantly improved the extent of esophageal damage caused by gastric acid reflux. Therefore, we suggested that costunolide has the potential to be a material of natural drug for the treatment of esophagitis caused by acid reflux. The study was supported by the Basic Science Research Program through the National Research Foundation (NRF) of Korea funded by the Ministry of Education (2017R1D1A3B03036020).

PNB-97

Antiallergic Effects of Family Cyperaceae Extracts

Hye Yoon Park1*, Gi-Hum Nam2, Sarah Lee3, Ga Ryun Kim1

1Biological and Genetic Resources Assessment Division, National Institute of Biological Resources, Incheon, Korea, 2Plant Resources Division, National Institute of Biological

Resources, Incheon, Korea, 3Biomaterials Research Group, National Institute of Biological Resources, Incheon, Korea

The Cyperaceae are a family of graminoid (grass-like), monocotyledonous flowering plants known as sedges, which superficially resemble the closely related rushes and the more distantly related grasses. The family is large, with some 5,500 known species described in about 90 genera, the largest being the “true sedges” genus carex with over 2,000 species. In this research, we prepared 12 species samples, the members of genus carex and cyperus. Carex is a vast genus of more than 2,000 species of grassy plants in the family Cyperaceae, almost worldwide in distribution. Cyperus is also a large genus of about 700 species of sedges, distributed throughout all continents in both tropical and temperate regions. We focused on the changes of IL-4 induced PMA/ionomycin (PI) and histamine secretion using RBL-2H3 cell (Rat Basophilic Leukemia Cell) and found that Cyperus globosus have the most powerful inhibition effects for production of IL-4 and also decreased the level of histamine release on RBL-2H3 cells and mast cells. From these results, we may suggest that Cyperus globosus might have useful as a anti-allergic material for functional food and pharmaceutics.

PNB-98

Chemical constituents of the culture broth of Antrodia albida

E-Eum Woo, Su Ha Lee, In-Kyoung Lee, Bong-Sik Yun*


The higher fungi produce structurally unique secondary metabolites with interesting biological activities. As part of an ongoing effort to discover novel fungal metabolites from the fungal strain of mushroom origin, we isolated three compounds from the culture broth of the fungus Antrodia albida. A. albida (Meripilaceae) grows well on the dead wood of deciduous trees and is distributed in Africa, Asia, and Europe. The fungus A. albida was cultured in potato dextrose broth medium at 27°C for 3 weeks with the agitation of 120 rpm. The whole culture broth was extracted with acetone and concentrated to eliminate acetone, followed by solvent partitioning with ethyl acetate. The ethyl acetate-soluble layer was concentrated and subjected to silica gel column chromatography, MPLC, Sephadex LH-20 column chromatography, and preparative reversed-phase HPLC to afford three compounds. Chemical structures of these compounds were determined by spectroscopic methods.

PNB-99

Chemical constituents of the culture broth of the fungus Panus rudis and their antioxidant activity

Lee Su Ha, E-Eum Woo, In-Kyoung Lee, Bong-Sik Yun*


Free radicals give rise to oxidative cellular damage, and the oxidation of biological molecules such as lipid, proteins, and DNA is thought to be responsible for the progression of many pathological processes including cancer, aging, inflammation, ischemia, and reperfusion. Thus, an intake of antioxidants has been posited to reduce the risk of developing some of these pathologies related with oxidative stress. In our continuing search for fungal metabolites with antioxidative activity, the culture broth of the fungus Panus rudis exhibited significant antioxidant activity. In this study, we present the isolation and structure determination of the antioxidative constituents from cultured broth of P. rudis. P. rudis was cultured in potato dextrose broth medium at 27°C for 4 weeks under stationary culture condition. The culture broth was extracted with acetone and filtrated. The filtrate was concentrated to eliminate acetone, and the resultant solution was partitioned with ethyl acetate. The ethyl acetate-soluble layer was subjected to silica gel column chromatography, MPLC, and preparative HPLC. Chemical structures of compounds were determined by mass and NMR spectroscopic methods.

Poster Presentation


Poster Presentation

PNB-104

Neuroprotective Ellagitannins against glutamate-induced apoptosis in HT22 cells

Ji Hoon Song1, Ji Yun Baek2, Hyun Young Kim2, Dahae Lee3, Do Hwi Park1, Ki Sung Kang1*

1College of Korean Medicine, Gachon University, 2Department of Food Science, Gyeongnam National University of Science and Technology, 3School of Pharmacy,

Sungkyunkwan University

Casuarinin andHT 22 cell death. These compounds block the accumulaton of intracellular reactive oxygen species (ROS) induced by glutamate. To evaluate the neuroprotective effects of hydrolysable tannins from T. chebula against glutamate-induced HT22 cell death. To demonstrate the underlying molecular mechanisms of the most effective compounds, casuarinin and chebulinic acid, against glutamate-induced HT22 cell death. Glutamate-mediated apoptotic HT22 cells death is dramatically diminished by either casuarinin or chebulinic acid. Casuarinin & chebulinic acid prevent the phosphorylation of mitogen-activated protein kinases (MAPKs) including ERK1/2 and p38. The T. chebula could be a natural source for the treatment of neurodegenerative diseases.

PNB-101

Diketopiperazines from the culture broth of the fungus Trametes palisotii

E-Eum Woo, Lee Su Ha, In-Kyoung Lee, Bong-Sik Yun*


Fungi produce structurally unique secondary metabolites with interesting biological activities. During the investigation on secondary metabolites of fungal strains of mushroom origin, we found that a fungal strain Trametes palisotii produced diverse metabolites in its culture broth. T. palisotii belongs to the polyporaceae family, and several Trametes species have been reported to exhibit antitumor and antigenotoxic activities. T. palisotii was cultured in potato dextrose broth medium for three weeks at 27°C with agitation of 120 rpm. The cultured broth was separated by solvent partition, silica gel column chromatography, medium-pressure liquid chromatography, Sephadex LH-20 column chromatography and preparative reversed-phase HPLC to afford four compounds. Chemical structures of these compounds were determined as diketopiperazines by the spectroscopic methods.

PNB-102

Anti-oxidant activities and anti-inflammatory effects of ethanol extracts from Anethum graveolens L. on

LPS-induced RAW 264.7 cells



Anethum graveolens L. (dill), is a kind of an annual herb from the Apiaceae family. The dill seeds as a traditional medicine was used to treat gastrointestinal diseases including flatulence, indigestion, stomach colic pain, and intestinal gas. In the present study, we evaluated the anti-oxidant activities and anti-inflammatory effects of ethanol extracts of A. graveolens (Ag). The anti-oxidant activities of Ag were measured by total contents of polyphenol, flavonoid, DPPH and ABTS radical scavenging and reducing power activity. And anti-inflammatory effects of Ag were measured by LPS-induced RAW264.7 cells. The DPPH radical scavenging activity was found to be 61.2% at 500 μg/ml. The ABTS radical scavenging and reducing power activity were more over 50% at 1000 μg/ml. The inhibitory effect of NO production was found to be 82% concentration 500 μg/ml of Ag. In addition, the generation quantity of inflammatory cytokines such as TNF-α and IL-1β in cell culture medium, the expression levels of inflammatory proteins in cells were showed decrease in a dose-dependent manner. Therefore, we suggest that the Anethum graveolens could be a potential material of anti-inflammatory drug with good anti-inflammatory effects. This work was carried out with the support of “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ014227)” Rural Development Administration, Republic of Korea.

PNB-103

Anti-oxidant activities and anti-inflammatory effects of ethanol extracts from Coriandrum sativum L. on

LPS-induced RAW 264.7 cells



Coriandrum sativum L(Cs)., an annual herbaceous plant of Apiaceae family. It is originated from Mediterranean area, and widely cultivated in North African and Asia as an edible and medicinal plant. In the present study, we evaluated the anti-oxidant activities and anti-inflammatory effects of ethanol extracts of Cs. The anti-oxidant activities of Cs were measured by total contents of polyphenol, flavonoid, DPPH and ABTS radical scavenging and reducing power activity. And anti-inflammatory effects of Cs were measured by LPS-induced RAW264.7 cells. The DPPH radical scavenging activity was found to be 52.8% at 500 μg/ml of Cs. The ABTS radical scavenging activity was 58.3% after exposure to 1000 μg/ml of Cs. Reducing power activity was found to be 66.8% at 2000 μg/ml. The inhibitory effect of NO production was found to be 65% concentration 500 μg/ml of Cs. In addition, the generation quantity of inflammatory cytokines such as TNF-α and IL-1β in cell culture medium, the expression levels of inflammatory proteins in cells were showed decrease in a dose-dependent manner. Therefore, we suggest that the Cs could be a potential material of anti-inflammatory drug with good anti-inflammatory effects. This work was carried out with the support of “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ014227)” Rural Development Administration, Republic of Korea.


224

PNB-108

Enhanced production of avenanthramide by elicitation method in oat sprouts

Tae Hee Kim1,2, Yu Jeong Jeong1, Suhyun Park1, Soyoung Kim1, Sung-Chul Park1, Saet Buel Lee1, Jang Won Pyun1, Jae Cheol Jeong1, Cha Young Kim1*,

Kwang Yeol Yang2

1Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, 2Department of Plant Biotechnology, College of Agriculture and Life Sciences,

Chonnam National University

Oat is an one of most valuable cereal which contains various healthy compounds such as soluble dietary fiber, high level of calcium, and low molecular weight phytoalexins known as avenanthramides. Among them, avenanthramides are unique soluble polyphenolic amides in oat and have benefits on human health such as antioxidant, anticancer, anti-itch and anti-inflammatory effects.In this study, to enhance avenanthramides contents in oat sprouts, we investigated the effect of various elicitors on the production of avenanthramide. Treatment with E2 resulted in a 2.5-fold higher content of avenanthramide (582.9 mg/kg in fresh weight, FW) than in an untreated control cultures (232.6 mg/kg, F.W). The addition of E3 produced a 2.8-fold enhancement of avenanthramide accumulation (642.9 mg/kg, F.W) in oat sprouts. Moreover, the avenanthramide production were also increased after co-treatment with E2 and E3 (1505 mg/kg, F.W) in oat sprouts. These co-treatment caused upregulation of phenylalanine ammonia-lyase (PAL) and Hydroxycinnamoyl-CoA : hydroxyanthranilate N-hydroxycinnamoyl transferase (HHT) genes. Taken together, we suggest that combination of E2 and E3 elicitor mediated approach gives offers a valuable system in enhanceing avenantramide contents in oat sprouts.

PNB-105

Inhibitory Effect of Cultivated Wild Ginseng Extract on TNFα/ IFNγ-induced inflammation of Human Keratinocyte cells

Dong Hee Kim1*, Jong Eel Park2, Jungeun Kim2, Jin Soo Kim2, Jun Ho Son2, Hyo Jung Kim2

1Department of Korean Medicine Development Industrialization Guidance Team, National Development Institute of Korean Medicine, Kyeongsan 38540, Republic of Korea,

2Department of Korean Medicine Development Korean Medicine R&D Team 1, National Development Institute of Korean Medicine, Kyeongsan 38540, Republic of Korea

Cultivated Wild ginseng (CWG) has been widely used as a panacea in traditional medicine. Several studies have shown that CWG treats cancerous tumors and inflammation-related diseases. However, the effects of CWG on inflammation and skin barrier activity in keratinocytes have not been investigated. In the present study, we evaluated the effects of CWG on TNFα/IFNγ-induced inflammation and skin barrier activity in HaCaT cells. The migration ability was examined by cell migration assay. The level of pro-inflammation related cytokines, COX-2 and skin barrier factor were measured by ELISA or Western blotting, respectively. CWG led to an significant increase in migration at the concentration of 100 μg/mL and inhibited the expression of TNF-α, IL-1β, and IL-8 in HaCaT cells. In addition, CWG induced the decrease in the expression of COX-2 and increase in the expression of filaggrin and loricirn. Taken together, our study demonstrates the availability of CWG as the materials for prevention of skin inflammation.

PNB-106

New Compounds from the Aerial Parts of Agastache rugosa and Their Anti-inflammatory Activity

Young Hye Seo1,2, Shin-Young Kang3, Ji-Sun Shin3, Seung Mok Ryu1, Byeong Cheol Moon1, Goya Choi1, Dongho Lee4, Kyung-Tae Lee3, Jun Lee1,2*

1Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, 2Convergence Research Center for Diagnosis, Treatment and Care System of Dementia,

Korea Institute of Science and Technology, 3Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, 4Department of Biosystems and Biotechnology,

College of Life Sciences and Biotechnology, Korea University

Five new compounds together with twenty-six known compounds were isolated from the aerial parts of Agastache rugosa (Fisch. & C.A.Mey.) Kuntze, Labiatae, by chromatographic methods. The chemical structures of new compounds were determined by the interpretation of spectroscopic data such as NMR (1H, 13C, HSQC, COSY, NOESY, and HMBC). Absolute configuration of new compound was determined by ECD study. Seven compounds were isolated from A. rugosa for the first time in this study. The potential anti-inflammatory activity of the isolates was evaluated by measuring their inhibitory activities on prostaglandin E2 production in lipopolysaccharide-treated RAW 264.7 macrophages. Through this study, we report the isolation, structural determination, and biological activities of chemical constituents from A. rugosa.

PNB-107

Enhancement of resveratrol production in peanut sprouts by elicitation method

Soyoung Kim1,2, Yu Jeong Jeong1, Suhyun Park1, Jae Cheol Jeong1, Bo-Keun Ha2, Cha Young Kim1*

1Biological Resource Center, Korea Research Institute of Bioscience Biotechnology, 2Department of Plant Biotechnology, College of Agriculture & Life Sciences,


Resveratrol is a major stilbene compound which is produced in several plants. Resveratrol benefits on human health such as antioxidant, anticancer, anti-heart disease, and anti-diabetes effects. Resveratrol is synthesized by the common phenylpropanoid pathway. Peanuts are a good source of resveratrol in nature and the peanut sprouts have higher resveratrol content than those in peanut seeds. In this study, to enhance resveratrol contents in peanut sprouts, We investigated the production of resveratrol in response to various elicitation. Expression of AhSTS gene was up-regulated by elicitation of 3-day-old sprouts with several elicitors including E2. In addition, E2 treatment significantly enhanced resveratrol content in 10-day-old peanut sprouts. HPLC analysis showed that resveratrol content was about 46-fold higher (44.2 μg/g in fresh weight, FW) in peanut sprouts treated with E2 for 4 days compared with control (0.9 μg/g FW). In addition, expression levels of AhPAL, AhC4H, Ah4CL, and AhSTS genes significantly increased in E2-treated peanut sprouts. Interestingly, epicotyls (460.1 μg/g in dry weight, DW) and hypocotyls (488.1 μg/g DW) had a higher trans-resveratrol content than shoots (2.0 μg/g DW) and roots (58.7 μg/g DW). Taken together, We suggest that elicitor (E2) mediated approach gives offers a valuable system in enhancing resveratrol content in peanut sprouts.

Poster Presentation


Poster Presentation

PNB-112

Sargassum Fulvellum Inhibits Inflammation and Oxidative Damage in Gluten-stimulated HaCat cells

Eun-Ji Shin1, Min Ju Kim1,2, Eui Jeong Han2, Disanayaka Mudiyanselage Dinesh Madusanka2, Mawalle Kankanamge Hasitha Madhawa Dias2, Ginnae Ahn2,3*

1Department of Research and Development, Nantapizza 5000, Damyang, Korea, 2Department of Food Technology and Nutrition, Chonnam National University, Korea,

3Department of Marine Bio-Food Sciences, Chonnam National University, Korea

In this study, we investigated the beneficial capacity of Sargassum Fulvellum (S. Fulvellum) against gluten-induced inflammation and oxdiative stress in HaCaT, a human keratinocyte. First of all, we prepared the hot water extract of S. Fulvellum (SFH) and used it for this study. SFH did not show cytotoxicities at the used all concentrations and improved the reduced cell viability as well as the increments of the intracellular reactive oxygen species (ROS) generation, the apoptotic body formation and the sub-G1 DNA population after the gluten stimulation. Also, SFH reduced the expression levels of inflammatory cytokines such as interleukin (IL)-1β, IL-4, IL-5, IL-6, IL-8, and IL-13. Moreover, SFH suppressed the nuclear factor (NF)-κB signaling by regulating the phosphorylation of IκB-α and NF-κB p65 as well as the translocation of NF-κB p65 into the nucleus. In addition, SFH inhibited the activation of extracellular signal regulated kinase (ERK)/P38 signaling in the gluten-stimulated cells. From these results, we indicate that SFH ameliorates the inflammation and oxidative stress caused by the exposure of gluten in HaCaT cells and might be a natural material for the improvement of gluten-caused various diseases. This work (Grants No. 2018-2810) was supported by Korea Association of University, Research Institute and Industry (AURI).

PNB-109

Protective Role of Cirsium japonicum var. maackii from Amyloid Beta25-35-induced Cellular Damage in C6 Glial Cells

Min Jeong Kim1, Ji Hyun Kim1, Sanghyun Lee2, Hyun Young Kim3, Eun Ju Cho1*1Department of Food Science and Nutrition & Kimchi Research Institute, Pusan National

University, Busan 46241, Korea, 2Department of Plant Science and Technology, Chung-Ang University, Anseong 17546, Korea, 3Department of Food Science,

Gyeongnam National University of Science and Technology, Jinju 52725, Korea

In the Alzheimer’s disease, the accumulation of amyloid beta (Aβ) is one of the major hallmarks. Accumulation of Aβ causes oxidative stress and neuronal inflammation through activation of glial cells. Cirsium japonicum var. mackii (CJM) is known to possess medicinal benefits including antioxidant and anti-inflammation effects. We also previously demonstrated that CJM has protective effect from hydrogen peroxide-induced oxidative stress in C6 glial cells. In this study, we investigated the effect and its mechanisms of CJM on Aβ25-35-induced cellular damage in C6 glial cells. We observed that treatment of Aβ25-35

decreased cell viability, and increased reactive oxygen species (ROS) formation. However, the ethanol (EtOH) extract and four fractions (n-butanol, ethyl acetate, chloroform, and n-hexane) from CJM elevated cell viability and reduced ROS formation, indicating that CJM attenuated oxidative stress by Aβ25-35. In Western blot analysis, treatment of CJM suppressed Aβ25-35-induced inflammation through down-regulation of inducible nitric oxide synthase, cyclooxygenase-2, interleukin-1β, and interleukin-6 protein expressions compared with Aβ25-35 – treated cells. Furthermore, Aβ25-35-induced apoptosis also diminished by CJM through down-regulation of B-cell lymphoma-2-associated X protein/B-cell lymphoma-2 ratio. Our results suggest that CJM protects glial cellular damage that is induced by Aβ25-35 via regulation of inflammation and apoptosis.

PNB-110

Combination of Carthamus tinctorius L. Seed and Taraxacum coreanum Protects from Cognitive Impairment under

Aβ25-35-Induced Alzheimer’s Disease Model

Mei Tong He1, Ji Hyun Kim1, Chan Hum Park2, Yu Su Shin2, Eun Ju Cho1*1Department of Food Science and Nutrition & Kimchi Research Institute, Pusan National University, Busan 46241, Republic of Korea, 2Department of Medicinal Crop Research,

National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong 369-873, Republic of Korea

The prevention and treatment of neurodegenerative diseases such as Alzheimer’s disease (AD) are attracted much attention. To expect the synergistic effect of herbal medicines, their combination has been applied for AD therapy. Carthamus tinctorius L. seed (CTS) showed protective effect against cell apoptosis and oxidative stress in recent studies. Additionally, Taraxacum coreanum (TC) attenuated inflammation, as well as the oxidative damage and cognitive dysfunction. This study evaluated the cognitive improvement in the mixture of CTS and TC (5:5) via a series of behavioral tests including T-maze, novel object recognition, and Morris water maze tests. Aβ25-35-injected mice were administered with the mixture at 50, 100 or 200 mg/kg for 14 days. Results showed that new route awareness and novel object recognition were improved by the administration of the combination. Furthermore, under the water maze test, it took shorter time to reach the hidden platform after 3 days training, and the combination treated groups spent more time in the target quadrant. On the other hand, the time to reach the exposed platform showed no significant differences among the groups, indicating that the improvement of cognitive function are not related to the visual perception or physical activity. These results suggested the synergistic effect of CTS and TC combination against Aβ25-35-induced cognitive impairment.

PNB-111

The Effects of Paeoniflorin on Lipopolysaccharide-induced Neuroinflammation and Cognitive Impairment in Mice

Hui Wen Meng1, Ah Young Lee2, Eun Ju Cho1*1Department of Food Science and Nutrition & Kimchi Research Institute, Pusan National

University, Busan 46241, Republic of Korea, 2Department of Food Science, Gyeongnam National University of Science and Technology, Jinju 52725, Republic of Korea

Neuroinflammation plays a crucial role in neurodegenerative disorders, such as Alzheimer’s disease (AD). Paeoniflorin (PF) is the major active compound of Paeonia lactiflora, which is a traditional medicine widely used in Asia countries. In this study, we investigated the neuroprotective effect of PF. PF (5 and 10 mg/kg) was injected in lipopolysaccharide (LPS)-treated mice for 21 days intraperitoneally. Behavioral tests including T-maze, novel object recognition and Morris water maze tests were carried out to observe the protective role of PF against memory deficit and cognition dysfunction. In the T-maze and novel object recognition tests, the abilities of new route awareness and novel object recognition were improved by the injection of PF compared with LPS treated control group. In addition, PF groups took shorter time to reach the hidden platform than the LPS treated control group in Morris water maze test. The results of behavioral tests indicated that PF possesses protective activity against cognitive impairment induced by LPS. Furthermore, PF inhibited NO formation in brain, kidney, and liver tissue, and also PF reduced AST, ALT levels in serum. These results suggest that PF improves memory dysfunction and cognition impairment in LPS-treated mouse model.


226

PNB-116

Variation of Furanocoumarins in the Angelica dahurica Root Extracts due to the Different Source of Plant Materials and

Extraction Methods Employed

Tan Steven Ryan Susanto, Jaehong Han*

Metalloenzyme Research Group, Department of Plant Science and Technology

The root part of Angelica dahurica Bentham et Hooker (AD), or also known as baekji or guritdae in Korea, is a traditional medicine widely used in Korea, Japan, and China for alleviating headache, toothache, nasal congestion and several skin diseases. Many studies have been conducted to identify the bioactive compounds composing AD. Furanocoumarins (FCs) are the major constituents of AD, possessing beneficial pharmacological activities such as anti-microbial and anti-inflammatory. However, variation in AD composition profile can be observed, making the examination and comparison of plant materials profile from different sources essential. In this study, three different AD root samples (AD1-3) were analyzed by TLC and UHPLC-DAD. One of the AD root extract (AD1) showed distinct profile on both TLC and UHPLC chromatogram compared to the other extracts and AD reference plant material from Korea Food and Drug Administration (KFDA). Oxypeucedanin, imperatorin, isoimperatorin, and two other unidentified peaks were found as major constituents of AD2 and AD3, but not in AD1. Additionally, extraction method as a factor affecting the efficiency of extraction process was also evaluated. Organic solvent extraction and supercritical CO2 fluid extraction were employed for the comparison of composition profile and extraction yield. The results will provide the information for the application of furanocoumarins from AD.

PNB-113

Inhibitory effects of 3,4-dihydroxybenzaldehyde isolated from Polysiphonia morrowii on IgE-mediated allergic response in

mast cell and passive cutaneous anaphylaxis animal mouse model

Eui Jeong Han1, Soo-Jin Heo2, Eun-A Kim2, Eun-Ji Shin3, Min Ju Kim1,3, Disanayaka Mudiyanselage Dinesh Madusanka1,

Mawalle Kankanamge Hasitha Madhawa Dias1, Ginnae Ahn1,4* 1Department of Food Technology and Nutrition, Chonnam National University, Yeosu, Korea, 2Jeju International Marine Science Center for Research & Education, Korea

Institute of Ocean Science & Technology (KIOST), Jeju, Korea, 3Department of Research and Development, Nantapizza 5000, Damyang, Korea, 4Department of Marine Bio-Food

Sciences, Chonnam National University, Yeosu, Korea

In this study, we investigated the anti-allergic effect of 3,4-dihydroxybenzaldehyde (DDB) isolated from Polysiphonia morrowii on IgE-mediated mast cell activation and passive cutaneous anaphylaxis (PCA) animal mouse model. The result showed that DDB did not show any cytotoxic effects at used all concentration (15.6, 31.3 and 62.5 μg/ml) in bone marrow-derived cultured-mast cells (BMCMCs). DDB markedly suppressed the degranulation of BMCMCs by reducing the release of β-hexosaminidase in a dose dependently manner. Also, DDB decreased the binding of IgE with FcεRI as well as the expression of FcεRI on cell in dose dependent manner. In addition, DDB reduced the mRNA expression levels and the secretion of proinflammatory cytokines interleukin(IL)-1β, IL-4, IL-5, IL-6, L-10, IL-13, interferon (IFN)-γ and/or tumor necrosis factor (TNF)-α by suppressing the activation of nuclear factor (NF)-κB signaling. In further study, the application of DDB significantly reduced the PCA reaction known as a type I allergic reaction in IgE stimulated and BSA-sentisized mice ear. Taken together, these results suggested that DDB has the anti-allergic effect and might be a natural material for the remedy of allergy. This work (Grants No. 2019017569) was supported by National Research Foundation of Korea in 2019.

PNB-114

Protective effects of Sargassum horneri in UVB-irradiated Human Keratinocyte

Min Ju Kim1,2, Eui Jeong Han1, Eun-Ji Shin2, Disanayaka Mudiyanselage Dinesh Madusanka1, Mawalle Kankanamge Hasitha Madhawa Dias1,

Kyounghoon Lee3, Ginnae Ahn1,4*1Department of Food Technology and Nutrition, Chonnam National University, Korea,

2Department of Research and Development, Nantapizza 5000, Damyang, Korea, 3Department of Marine Production Management, Chonnam National University, Korea,

4Department of Marine Bio-Food Sciences, Chonnam National University, Korea

In this study, we evaluated the protective activity of chloroform fraction of Sargassum horneri methanol extract (SHMC) against UVB-irradiated cellular damages in HaCaT cells, a human keratinocyte. The results showed that SHMC improved the cell viability in UVB-irradiated HaCaT cells by reducing the intracellular reactive oxygen species (ROS) generation. In addition, SHMC inhibited apoptosis via the reduction in sub-G1 cell population and apoptotic body by the modulating of Bax/Bcl-2 expressions known as apoptosis-related molecules in UVB-irradiated HaCaT cells. Furthermore, SHMC suppressed the NF-κB signaling by regulating the phosphorylation of IκB-α and NF-κB as well as the activation of nuclear factor (NF)-Κb p65. Interestingly, the pre-treatment with SHC led to the activation of Nrf2/HO-1 signaling with the increased heme oxygenase 1 (HO-1) and the nuclear factor erythroid 2-related factor 2 (Nrf2) expressions. The protective capacities of SHMC against UVB-caused cellular damages were blocked by the inhibition of HO-1 singaling. Taken together, this study suggests that SHMC may be used as a potential protector against damage caused by UVB irradiation. This work (Grants No. M01201920180359) was supported by Korea Institute of Marine Science & Technology Promotion (KIMST).

PNB-115

Effect of Aster yomena (Kitam.) Honda against Obesity and Insulin Resistance in High Fat Diet-fed Mouse Model

Min Jeong Kim1, Jeong Sook Noh2, Sanghyun Lee3, Eun Ju Cho1, Hyun Young Kim4*

1Department of Food Science and Nutrition, Pusan National University, Busan 46241, Korea, 2Department of Food Science and Nutrition, Tongmyong University, Busan 48520,

Korea, 3Department of Plant Science and Technology, Chung-Ang University, Anseong 17546, Korea, 4Department of Food Science, Gyeongnam National University of Science

and Technology, Jinju 52725, Korea

Obesity is associated with the development of metabolic dysfunction in the body, such as deteriorated lipid profile and insulin resistance. Until now, there exists no effective anti-obesity medication without side effect. The purpose of this study was to examine whether Aster yomena (Kitam.) Honda (AY), that has been used as a medicinal herb in Korea for antibacterial and anti-inflammatory effects, improves obesity and insulin resistance in vivo. When ethyl acetate fraction from AY (EFAY) was administered to C57BL/6J mice fed with 60% high fat diet (HFD), EFAY significantly lowered body and organ weights compared to the HFD control mice. EFAY groups also improved metabolic dysfunctions including serum lipid profile, fasting blood glucose, and serum insulin compared to HFD control group. We further elucidated the mechanisms from obesity and insulin resistance by Western blot analysis. In the liver, EFAY significantly suppressed lipogenesis-related protein expressions including fatty acid synthase and acetyl-CoA carboxylase (ACC). EFAY promoted activation of AMP-activated protein kinase (AMPK) pathway by regulating phosphorylated AMPK and phosphorylated ACC expressions, which could improve lipid and glucose metabolism in the liver. Taken together, EFAY has anti-obesity and blood glucose control effects in the high fat diet-fed mouse model. Therefore, EFAY is responsible for a promising agent against obesity and insulin resistance.

Poster Presentation


Poster Presentation

PNB-120

Effects of Heat-killed Enterococcus faecalis EF-2001 on Ethanol-induced Acute Gastric Mucosal Injury in Mice

Han Gyo Shin, Da Bin Jeon, Ba Wool Lee, Seong Hun Jeong, Ju Hong Kim, Ji Hye Ha, Ji Young Park, Hyung Jun Kwon, Young Bae Ryu, In Chul Lee*


This study aimed to investigate the protective effects of heat-killed Enterococcus faecalis EF-2001 (EF-2001) on gastric ulcer using a murine model of ethanol (EtOH)-induced acute gastric injury. EF-2001 (20, 40, and 80 mg/kg/day) was administered by oral gavage for 5 days before EtOH treatment (10 mL/kg body weight). EF-2001 effectively attenuated EtOH-induced gastric mucosal injury with reduced gastric mucosal ulcer and histological damage score. Pre-treatment of EF-2001 markedly suppressed the phosphorylation of mitogen-activated protein kinases (MAPKs; ERK1/2, JNK, p38MAPK). In addition, EF-2001 significantly inhibited phosphorylation of nuclear factor-kappaB (NF-κB) and subsequently suppressed the up-regulation of inducible nitric oxide synthase, cyclooxygenase-2, tumor necrosis factor-α, interleukin-1β and interleukin-6 in gastric tissues. Taken together, these results suggest that EF-2001 exerts a gastroprotective effect against acute gastric injury, and the underlying mechanism might be associated with the suppression of MAPKs and NF-κB signaling and consequent reduction of pro-inflammatory mediators or cytokines.

PNB-117

Anti-Melanogenic Effect of Gedunin in B16F10 Melanoma Cell Line

Hwang-Ju Jeon, Yong-Chan Kim, Hye Seung Choe, Sung-Eun Lee*


Gedunin known as a natural Hsp90 inhibitor in tetranortriterpenoids has been found in Indian neem tree and has exhibited its biological activities, including anticancer activity, protective effect on the TLR-mediated inflammation, and antimalarial activity against Plasmodium falciparum. Herein, we evaluated anti-melanogenic effect of gedunin using B16F10 mouse melanoma cell line. Melanogenesis was stimulated by the treatment of alpha melanocyte stimulating hormone (α-MSH, 200 μM) and two doses of gedunin, 25 and 50 μM, were treated into the medium containing the cells after 1 h later the addition of α-MSH. mRNA levels and intracellular melanin contents were measured after 24 h and 48 h incubations. The mRNA level of Mitf gene in α-MSH-treated cells was 1.5-fold up-regulated, and the stimulating effect decreased by the addition of gedunin. The mRNA level of Mitf gene in the gedunin-treated group was significantly reduced when compared to α-MSH-treated group. Melanin production in melanoma cells was induced after 48 h of α-MSH treatment, and intracellular melanin contents were about 3-fold higher than the control group. Stimulated melanin amounts by α-MSH decreased by gedunin treatments, and the remaining intracellular melanin contents were 0.4 and 0.44-fold lower than α-MSH-treated group, respectively. Therefore, gedunin attenuates melanogenesis in B16F10 melanoma cell line and it is highlighted to use it as a whitening ingredient in cosmetic industry.

PNB-118

Chemical constituents of the rhizome of Atractylodes macrocephala

Jin Hong Guang1, Hwan Lee2, Hye Jin Kim1, Kwan-Woo Kim1, Dong-Sung Lee2, Dae Young Lee3, Dahye Yoon3, Geum-Soog Kim3, Ren-Bo An4,

Hyuncheol Oh1, Youn-Chul Kim1*1College of Pharmacy, Wonkwang University, Iksan 54538, Republic of Korea, 2College of Pharmacy, Chosun University, 309 Pilmun-daero, Dong-gu, Gwangju 61452, Republic of

Korea, 3Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, Republic of Korea, 4Key Laboratory of

Natural Resources of Changbai Mountain & Functional Molecules, (Yanbian University), Ministry of Education, Yanji, Jilin 133002, P.R. China

Atractylodes macrocephala is a famous medicinal plant, and its rhizome has long been used as a tonic agent in various ethno-medical systems in East Asia including Korea, Japan and China for the treatment of gastrointestinal dysfunction, cancer, osteoporosis, and obesity. A. macrocephala rhizome has been reported to possess diverse biological activities, such as anti-tumor, anti-inflammatory, anti-aging, anti-oxidative, and anti-bacterial activities. A. macrocephala rhizome has been reported to contain sesquiterpenoids, polyacetylenes, phenylpropanoids, flavonoids, and polysaccharides. In the present study, by means of repeated column chromatography using silica gel, Sephadex LH-20 and ODS, two new compounds along with sixteen known compounds. The structures of the known compounds were identified as atractylone (1), 3β-acetoxyatractylon (2), atractylenolide I (3), atractylenolide II (4), atractylenolide III (5), atractylenolide VII (6), 8-epiatractylenolide III (7), eudesm-4(15)-ene-7α,11-diol (8), linoleic acid (9), myristic acid (10), 3-O-caffeoyl-1-methylquinic acid (11), (2E,8E,10E)-tetradecatriene-4,6-diyne-1,14-diol (12), 14-aceroxy-12-senecioyloxytetradeca-2E,8Z,10E-trien-4,6-diyn-1-ol (13), isoscopoletin (14), caffeic acid (15), and protocatechic acid (16). The structures of all the isolated compounds were identified based on 1D and 2D NMR, including H-H COSY, HSQC, and HMBC spectroscopic analyses.

PNB-119

Alleviating effect of the Chrysanthemum zawadskii Herbich var. latilobum Kitamura extract via inhibiting prostaglandin F2α

(PGF2α) on oxytocin-induced primary dysmenorrhea in mice

Ju Hong Kim1, Ji Hoon Lee1, In Chul Lee1, Young Bae Ryu1, Seong Hun Jeong1, Da Bin Jeon1, Han Gyo Shin1, Ba Wool Lee1, Ji Hye Ha1,

Kyoung Oh Cho2, Hyung Jun Kwon1*1Functional Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology, 2Laboratory of Veterinary Pathology, College of Veterinary Medicine,


Primary dysmenorrheal, which is a common gynecological problem, is defined as cramping pain in the lower abdomen occurring just before or during menstruation, and it occurs in up to 50% of menstruating females and causes significant disruption in quality of life. Chrysanthemum zawadskii (CZ), known as “Gujulcho” in Korea, has been used in traditional medicine to treat various inflammatory diseases, including rheumatoid arthritis. The aim of this study was to determine the inhibitory effect of CZ on the release of uterine prostaglandin F2α (PGF2α), which is associated with primary dysmenorrhea. Here, we investigated the effects of C. zawadskii water extract (CZW) on the analgesic activities were studied by measuring nociception using oxytocin-induced writhing in estrogen-treated mouse models. Prostaglandin E2 and nitric oxide production in cultured lipopolysaccharide (LPS)-treated macrophage cells were determined. The level of PGF2α in uterine tissue were determined according to specification of enzyme linked immunosorbent assay (ELISA) and histopathological analysis (H&E). In vivo study demonstrated that the CZW significantly reduced OT-induced writhing responses. It also decreased the level of PGF2α in OT-induced mice uterine tissue. Moreover, Western blot analysis showed that COX-2 and OTR expressions in uterine tissue of dysmenorrhea mice were significantly reduced. These results indicate that C. zawadskii water extract (CZW) decreased the release of PGF2α and may be a therapeutic candidate for the treatment of primary dysmenorrhea.


228

PNB-124

Anti-melanogenic effect of callus extract from Pinellia ternata in α-MSH stimulated B16F10 Cells

Seung Bin Park, Jong Sub Lee, Min Ju Lim, Jae In Lee, Dong Young Kim, Ji Hoon Lee, Hyung Jun Kwon, In Chul Lee, Ji Young Park, Young Bae Ryu*


Pinellia ternata is one of the most commonly used herbs in traditional Chinese medical science. P. ternata can be used for treating cough, vomiting, headache, and dizziness, and so on. In recent years, the therapeutic effects of P. ternata on tumors have been further demonstrated by modern pharmacological investigations. In the present study, we found novel anti-melanogenic properties of methanolic extract of P. ternata callus. Anti-melanogenic activity of P. ternata callus (EC50 =17.2 μg/ml) is much more effective than synthetic tyrosinase inhibitor known as kojic acid (EC50=64.96 μg/ml) and we also checked the cytotoxic concentration of P. ternata callus (CC50=139 μg/ml). To further elucidate the anti-melanogenic effect of P. ternata callus, we performed depigmenting assays using the zebrafish embryo model. The body pigmentation of zebrafish was visualized using a Leica Microsystems and quantified by NIH image J software (National Institutes of Health, Bethesda, MD, USA). Furthermore we checked the mRNA expression of TRP-1, TRP-2, tyrosinase and, microphathalmia-associated transcription factor (MITF) by using real-time PCR. Collectively, P. ternata callus extracts could be a candidate agent for treating hyperpigmentation and additive to whitening cosmetics.

PNB-121

Spiraea prunifolia var. simpliciflora attenuates inflammatory responses in a murine model of lipopolysaccharide-induced

acute lung injury via suppression of MAPK and NF-κB

Ba Wool Lee, Han Gyo Shin, Seong Hun Jeong, Da Bin Jeon, Ju Hong Kim, Ji Hye Ha, Ji Young Park, Hyung Jun Kwon, Young Bae Ryu, In Chul Lee*


Spiraea prunifolia var. simpliciflora (SP) is a deciduous shrub that grows in most regions of Korea. The root of SP is traditionally used as an herbal for anti-pyretic, anti-malaria, and emetic treatment. This study aimed to investigate the anti-inflammatory response of the methanol extract of SP leaves in tumor necrosis factor (TNF)-α-stimulated NCI-H292 cells and in LPS-induced acute lung injury (ALI) mice. The C57BL/6 mice were administered 50 and 100 mg/kg of SP by oral gavage for 5 days. LPS was applied to animals via intranasal inoculation 1 h after the final SP treatment. In the TNF-α-stimulated NCI-H292 cells, SP significantly decreased TNF-α, interleukin-1β (IL-1β), and IL-6 in a concentration-dependent manner. SP also significantly suppressed the phosphorylation of mitogen-activated protein kinases (MAPKs; JNK, Erk, and p38) and nuclear factor-kappaB (NF-κB). In the ALI model, SP decreased the inflammatory cell count and TNF-α, IL-1β, and IL-6 levels in the bronchoalveolar lavage fluid and inflammatory responses in the lung tissues of SP-treated mice compared with the ALI-induced mice. SP also effectively suppressed phosphorylation of the MAPKs and activation of p65NF-κB in lung tissues. Overall, our results indicate that SP has therapeutic effects on inflammatory responses in TNF-α-stimulated NCI-H292 cells and in the LPS-induced ALI model by inhibiting activation of the MAPKs and NF-κB pathways concurrent with suppressing production of pro-inflammatory cytokines.

PNB-122

Ameliorative effect of Ficus auriculata Lour. extract in testosterone-induced benign prostatic hyperplasia (BPH)

Jae In Lee, Jong Sub Lee, Min Ju Lim, Seung Bin Park, Dong Young Kim, Ji Hoon Lee, Hyung Jun Kwon, In Chul Lee, Ji Young Park, Young Bae Ryu*


Benign prostatic hyperplasia (BPH) can lead to lower urinary tract symptoms. In this study, we investigated whether Ficus auriculata Lour. (FA) extract affects testosterone-induced benign prostatic hyperplasia (BPH) and metabolites profiling using ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS). 21 metabolites in FA extract were identified by ESI-negative mode. We also evaluated prostate specific antigen (PSA) expression assay and androgen receptor (AR). FA extract reduced the expression of BPH markers including PSA (prostate specific antigen) and AR (androgen receptor). These findings suggest that FA extract effectively prevented the development of testosterone-induced BPH and might be useful in the clinical treatment of BPH.

PNB-123

Inhibitory Effect of Callus Extract from Euphorbia maculate on Melanogenesis in B16F10 Cells

Jong Sub Lee, Min Ju Lim, Jae In Lee, Seung Bin Park, Dong Young Kim, Ji Hoon Lee, Hyung Jun Kwon, In Chul Lee, Ji Young Park, Young Bae Ryu*


Previously we demonstrated anti-melanogenic effect of the methanolic extracts of Euphorbia maculate callus (EM) on α-MSH stimulated melanin formation in B16F10 mouse melanoma cells. The results demonstrated that extracts of E. maculate callus (EC50 =21.7 μg/mL) showed much better inhibition of melanogenesis than kojic acid used as a positive control (EC50=64.96 μg /mL). We investigated the expression of the critical molecules involved in melanin synthesis such as TRP-1, TRP-2 and microphthalmia-associated transcription factor (MITF) was attenuated dose-dependently in callus extracts-treated cells. Protein and mRNA expressions of these molecules were measured by western blot analysis and reverse-transcription polymerase chain reaction (qRT-PCR). We also checked anti-melanogenic effect of methanolic extracts of EM whole plants. Methanolic extracts of E. maculate whole plants showed more cytotoxicity with lower anti-melanogenic effect. Furthermore, metabolites in E. maculate callus extracts and whole plants extracts are evaluated by using Ultra-Performance Liquid Chromatography-Quadrupole-Time-of-Flight (UPLC-QTOF) mass spectrometry (MS) analysis. Taken together, the extract of E. maculate callus may partly accounted for the whiting products and drugs for skin disorders.

Poster Presentation


Poster Presentation

PNB-128

C-phycocyanin containing multifunctional chitosan-coated poly(lactic-co-glycolic acid) nanoparticles to treat atopic

dermatitis by spatiotemporally controlled codelivery of ceramide

Seul Ki Min1*, Hoo Cheol Lee2, Hwa Sung Shin2

1Division of Polar Life Science, Korea Polar Research Institute, 2Department of Biological Engineering, Inha University

Atopic dermatitis is the most common and complex inflammatory disease associated with defective skin barrier. Therefore an anti-inflammatory agent is required to effectively treat atopic dermatitis with co-administration of a lipid-enhancing skin barrier composed of ceramide. Treatment of atopic dermatitis will benefit from the development of nanoparticles with long-term efficacy in a single dose, as the treatment is affected by drug dose and frequency. In this study, skin-sensitive chitosan nanoparticles delivering both ceramide and C-phycocyanin have been developed to exhibit anti-inflammatory effects and their effects on the stratum corneum formation in a rat atopic dermatitis model was verified. Analysis of mRNA expression of keratinization factors showed that the stratum corneum formation effect of poly(lactic-co-glycolic acid) nanoparticles containing ceramide was similar to or higher than that of ceramide alone. Nanoparticles coated with C-phycocyanin and chitosan reduced histamine release compared to nanoparticles without C-phycocyanin. Additionally, despite frequent washing, the nanoparticles were fixed well to the epidermis after their administration. Collectively, the combination of lipid therapy/anti-inflammatory nanoparticles could be used as a treatment strategy for atopic dermatitis, providing antihistamine and keratinization effects after a single dose of their administration.

PNB-125

Effect of Sapium rotundifolium hemsl extract on testosterone-induced benign prostatic hyperplasia

Min Ju Lim, Hyung Jun Kwon, Jong Sub Lee, Jae In Lee, Young Bae Ryu, Ji Young Park*


Benign prostatic hyperplasia (BPH) is an extremely common chronic disease in the male population, in which the incidence increases gradually with age, and almost 50% of men over the age of 50 suffer from this disease. In this experiment, the methanolic extract of Sapium rotundifolium (SRM) was fractionated into hexane (SRH), chloroform (SRC), ethyl acetate (SRE), butanol (SRB) and H2O (SRD). Among these sub-fraction layers, SRH and SRC decreased the mRNA expression of PSA and AR in LNCaP-LN3 cells. Also, SRM, SRH and SRC reduced the expression of BPH-related proteins such as androgen receptor (AR), prostate specific antigen (PSA), 5 alpha reductase 2 (5αR-2), estrogen receptor alpha (ERα) and steroid receptor coactivator 1 (SRC-1) in LNCaP-LN3 cells. In order to find active compounds, the metabolites of SRM, SRH and SRC were analyzed by UPLC-QTOF/MS and identified nine common compounds. SRM was assessed in testosterone propionate (TP)-induced BPH rat model. The treatment of SRM decreased the prostate weight and prostate weight index, and also decreased the levels of testosterone and dihydrotestosterone (DHT) in serum. In addition, the expression levels of AR, PSA, 5αR-2, ERα and SRC-1 proteins in the SRM treatment group were significantly reduced. Histological analysis also showed that the SRM treatment suppressed TP-induced prostatic hyperplasia. These results suggest the possibility of S. rotundifolium as a pharmacological therapy for BPH.

PNB-126

Combination of GU-10 water extract and curcumin exhibits antiviral activity against porcine epidemic diarrhea virus

infection in piglets

Da Bin Jeon1, In Chul Lee1, Seong Hun Jeong1, Ji Young Park1, Young Bae Ryu1, Joo Hong Kim1, Han Gyo Shin1, Ba Wool Lee1, Ji Hye Ha1, Kyoung Oh Cho2,

Woo Song Lee1, Hyung Jun Kwon1*1Functional Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology, 2Laboratory of Veterinary Pathology, College of Veterinary Medicine,


Porcine epidemic diarrhea virus (PEDV) causes severe watery diarrhea and lead to death in piglets resulting economic losses of porcine industry. Therefore, a highly effective antiviral strategy against PEDV outbreaks is needed. In this study, we investigated whether combinations of GU-10 water extract and curcumin exerted antiviral activity against PEDV by inhibiting viral replication and host inflammation in vitro and in vivo. In vitro post-treatment assay which measure the ability to inhibit viral replication, GU-10 had antiviral activities of a 50% effective inhibitory concentration (EC50) with a dose of 310.5 mg/mL and a selectivity index (SI) value of 1.3. Interestingly, combination of GU-10 and curcumin in a ratio of 9:1 exhibit stronger antiviral activities of an EC50 with a dose of 37.1 mg/mL and a SI value of 2.9. Moreover, combination ratio of 9:1 showed effective anti-inflammatory activities much as curcumin on PEDV infection. To confirm the antiviral activities, in vivo transmission disease model in piglets were performed. Piglets infected with PED virus expressed watery diarrhea, vomiting, and dehydration within two days. These results suggest that combination of GU-10 and curcumin in a ratio of 9:1 have strong antiviral activity against PEDV and may be developed into natural therapeutic drugs.

PNB-127

Anti-allergic inflammatory effect of alendronate in mast cells

Jihye Lee1, Eui Jeong Han2, Ginnae Ahn2, Kyungsook Jung1*1Functional Biomaterial Research Center, Korea Research Institute of Bioscience and

Biotechnology, 2Department of Marine Bio-Food Sciences, Chonnam National University

Nitrogen-containing bisphosphonates (NBPs), such as alendronate, are inhibitors of mevalonate pathway, and has been widely used for the treatment of osteoporosis. Recent studies suggest NBPs exhibit inhibitory effects on growth and function of various immune cells. Mast cells are known as key player in IgE-dependent allergic reaction and other several immune responses. We have evaluated the inhibitory effect of alendronate on IgE-mediated mast cell activation. Bone marrow-derived cultured mast cells (BMCMCs) from C57BL/6 mice were used to determine the release of β-hexosaminidase. Flow cytometer analysis was conducted to evaluate the effect of alendronate on mast cell activation, through the detection of FceRI expression and IgE-binding to the surface of BMCMCs. We performed RT-PCR to investigate the effect of alendronate on IgE/BSA-induced cytokine production in BMCMCs. To evaluate the effect of alendronate on mast cell-mediated hypersensitivity reaction in vivo, passive cutaneous anaphylaxis (PCA) mice model induced by both anti-2,4-dinitrophenol(DNP)-IgE and DNP-BSA. Pre-incubation of BMCMC with alendronate resulted in dose-dependent inhibition of anti-IgE-induced release of beta-hexosaminidase. IgE-mediated inflammatory cytokine and chemokine mRNA expression was also reduced. Mast cell-mediated hypersensitivity was significantly reduced on alendronate treated PCA mice model. These results suggest that alendronate act as modulator of mast cell activation through inhibition of IgE receptor cross-linking, thus indicating the presence of new targets of alendronate.


230

PNB-129

Gyeonggi-do Beauty Resource Bank [Cosmetic resource DB construction project]

Changon Seo, Jin Kyu Kim, Woo Jung Kim, Mi Suk Seo, Jung A Lee, Young Rak Cho, Kyu Hee Park, Jong Suk Lee*

Bio-Center, Gyeonggido Business & Science Accelerator (GBSA)

The Gyeonggi-do Beauty Resource Bank collects specialized bioresources from the Gyeonggi province, builds a database for each sample, established an extract library, and provides samples to companies.The beauty resource DB supports industrialization by providing various information such as scientific name, extraction information, compound information, and LC-MS profile about the materials. The plant samples from Gyeonggi-do and DMZ area are the first choice for the bioresource. Up to now, the database has been established for 500 samples, and it is expanding to 700 samples within the year 2019. We are ready to provide 400 samples at present.All information about DB and resource are provided in the homepage(www.g-beauty.or.kr).

PNB-130

Investigation of Drug-herbs and Anti-hypertensive Drugs in Human Hepatocytes

Jin-Moo Lee1, Sun-Young Ahn1, Young-Hoon Kim1, Hye Jin Cha1, Jisoon Shin1, Hye Hyun Yoo2, Soo Kyung Suh1*

1Pharmacological Research Division, Toxicological Evaluation and Research Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety,

187 Osong Saengmyeong 2-ro, Heungdeok-gu, Chungju-shi 28159, Republic of Korea, 2College of Pharmacy, Hanyang University, 55 Hanyang University-Ro, Sangnok-go,

Ansan-si, Republic of Korea

Drug-drug interactions studies provide important clues to safe use of pharmaceuticals, as in general, drug use are involved with multiple drug combinations. In this study, we evaluated drug interactions of two herbal ingredients (Salvia miltiorrhiza Bunge; salvianolic acid B and Lycium chinense Mill; betaine) and anti-hypertensive drugs in human hepatocytes. Extract of Salvia miltiorrhiza Bunge and Lycium chinense Mill are known as anti-hypertensive agents. The aim of this study was to evaluate drug-herb interaction using measurement of metabolic enzyme cytochrome P450. As a result, salvianolic acid B inhibited the activities of CYP2C9 and CYP3A4, while betaine induced CYP2C9 and CYP3A4. E-3174 is metabolite of losartan which was metabolized by CYP2C9 and/or CYP3A4. When salvianolic acid B was used in combination with losartan, the production of E-3174 was decreased in a dose-dependent manner. On the other hand, the combination of betaine and losartan increased E-3174 production in a dose-dependent manner. This suggests a potential change in the blood level of these drugs when taken simultaneously with Salvia miltiorrhiza Bunge, and Lycium chinense Mill, indicatory potential adverse effects.

Poster Presentation


Poster Presentation

PES-4

Influence of heading date difference on gene flow from PAC rice to rice cultivars

Eunji Bae, Doh-Won Yun, Gang-Seob Lee, Ancheol Chang, Sung-Dug Oh*


Genetically modified (GM) crops have been increased continuously over the world and concerns about the potential risks of GM crops have also been increasing. In this study, we investigated the influence of heading date difference on gene flow from PAC rice to rice cultivars. In the experimental plot design, The PAC rice was placed in the center as a pollen donor and rice cultivars were placed in eight directions as pollen receivers. Five pollen receiver rice cultivars were Unkawng, Daebo, Saegyejinmi, Nakdong, and Ilmi which had different flowering times. A total of 266,436, 300,237, 305,223, 273,373, and 290,759 seeds were collected from Unkawng, Daebo, Saegyejinmi, Nakdong, and Ilmi, respectively, which were planted around PAC rice. The PAC×non-GM hybrids were detected by repeated spraying of herbicide and PAT immunostrip assay. The hybrids were found in Nakdong which had the same heading date with PAC rice. The hybridization rate was 0.0007% at Nakdong plot. The physiological elements including rice heading date were found to be important factors to determine PAC rice out crossing rate with PAC rice. Consideration should be taken into for many factors like the physiological elements of field heading date of rice cultivars to set up the safety management guideline for prevention of GM rice gene flow.


PES-1

Land Suitability Assessment of Apples and Pears by Combining Classification Results by Climate and Soil

Information in South Korea

Yong Seok Kim*, Kee Kyung Kang, Kyo Moon Shim, Ji Na Hur


Land suitability assessment for apples and pears conducted with soil and climate information in south Korea. In doing so, we intended to preserve land and increase the productivity by providing valuable information regarding where more suitable areas for apples or pears are located. For the analysis of land suitability assessment for apples and pears soil factors and climte factors related to apples and pears growth were selected. Soil morphological and physical properties including subsoil texture, degree of soil erosion, drainage class, available soil depth, slope, gravel content and topography were selected as soil factors. Climate factors including average annual temperature, temperature during the growing season, temperature during maturation, extreme minimum temperature, chilling requirement and precipitation were selected. And we combined both soil and climate classification results using a most-limithing characteristic method. Maps showing the suitable land for ginseng cultivation were drawn, adopting the land suitability classes obtained through this study, soil map, climate map, and GIS information. The results indicate that the suitable area of cultivation for apples in south Korea was 3.5% and them for grape was 15.1%.

PES-2

Isolation and characterization of Pseudomonas geniculate ANG3, Exiguobacterium acetylicum ANG40 and Burkholderia

stabilis ANG51 isolated from soil

Hee Sook Kim1, Ji-Youn Kim1, Song Min Lee1, Hye-Jung Park1, Sang-Hyeon Lee2, Jeong Su Jang1*, Mun Hyon Lee1

1Food Research Center, Angel Co., Ltd., 2Major in Pharmaceutical Engineering, Division of Bioindustry, Silla University

This study was carried out to investigate plant growth promoting activity and plant disease controlling activity using strains isolated from soil. According to the result of study on of plant growth promoting activity, ANG51 was found to have 1-aminocylopropane-1-carboxylyic acid (ACC) deaminase and Indole-3-acetic acid (IAA) production ability related to ethylene. In contrast, the other strains were found to have only IAA production ability. In addition, all the strains were found to show abilities of nitrogen fixation, phosphate solubilization, and siderophore production. Also, it was confirmed that they could grow in various growth temperature ranges. According to the analysis on plant disease controlling effect, all the strains except ANG40 showed antagonistic action against various phytopathogenic fungi, which can be ascribed to siderophore production ability and antibiotic substances. In the case of ANG40, it was found to have extracellular activity. Although ANG40 failed to show antagonistic action against phytopathogenic fungi, it has a significant meaning, given that it can help plant growth by converting macro molecules, which cannot be used for the growth of plants, into small molecules. Therefore, this study suggested the possibility of using Pseudomonas geniculate ANG3, Exiguobacterium acetylicum ANG40, Burkholderia stabilis ANG51, which were finally selected from each group through comparative advantage analysis on plant growth promotion and plant disease controlling effect, as biological agents.

PES-3

Molecular biological analysis of Bt-transgenic (Bt-9) rice and its effect on Daphnia magna feeding

Jiyoung Kim, Soo-Yun Park, Doh-Won Yun, Myung-Ho Lim, Ancheol Chang, Sung-Dug Oh*


Insect-resistant transgenic (Bt-9) rice was generated by inserting mCry1Ac1, a modified gene from the soil bacterium Bacillus thuringiensis, into the genome of a conventional variety of rice (Ilmi). With regard to potential problems such as safety, an evaluation of non-target organisms is necessary as an essential element of an environmental risk assessment of genetically modified (GM) crops. We studied the effects of the Bt-9 rice on the survival of cantor Daphnia magna, a commonly used model organism in ecotoxicological studies. D. magna fed on the Bt-9 rice and its near non-GM counterparts (Ilmi) grown in the same environment. The Bt-9 rice was confirmed to have the inserted T-DNA and protein expression evident by the PCR and ELISA analyses. The feeding study showed a similar cumulative immobility and abnormal response of the Daphnia magna between the Bt-9 rice and Ilmi. Additionally, the 48 h-EC50 values of the Bt-9 and Ilmi rice were 4,400 mg/L (95% confidence limits: 3861.01-5015.01 mg/L) and 5,564 mg/L (95% confidence limits: 4780.03-6476.93 mg/L), respectively. The rice NOEC (No observed effect concentration) value for D. magna was suggested to be 1,620 mg/L. We conclude that the tested Bt-9 and Ilmi have a similar cumulative immobility for D. magna, a widely used model organism, and the growth of Bt-9 did not affect non-target insects.


232

PES-8

Effect of different types of UV radiation on Triton X-100 degradation

Dong Gon Yoo, Seong Ho Yun*, Eun Hea Jho*

Department of Environmental Science, Hankuk University of Foreign Studies

The pesticide products contain surfactants, which are used for effective activation of the pesticide ingredients. Some studies have reported that surfactants may exhibit similar or higher lethal effects than pesticide ingredients. In this study, Triton X-100 (TX) was used as a target chemical and 35 d-photolysis experiments were carried out using different UV wavelengths. The residual TX concentrations were analyzed by HPLC and intermediate products were analyzed by NMR. Toxic effects were determined using Microtox. The TX reduction in the controls was insignificant. TX was reduced by 25% with UV-A, 81% with UV-B, and 96% with UV-C within one day. The NMR spectrums were also different for different types of UV radiations. The toxic effects of photodegraded samples were greater than that of the controls. The samples irradiated by UV-C showed higher toxicity than the samples irradiated by the other UV radiations. The increase in toxicity after photodegradation suggests that intermediate products with higher toxicity might have been generated during photodegradation. Overall, this study shows that the toxicity of surfactants, which can enter the environment in various ways, can change due to photodegradation in the environment.

PES-5

Catalytic Activity of Azo Dye Orange G in Heterogeneous Fenton Oxidation with Fe-Impregnated Biochar Catalyst

Jong-Hwan Park1, Seong-Heon Kim2, Dong-Cheol Seo1*1Division of Applied Life Science (BK21 Plus) & Institute of Agriculture and Life Science,

Gyeongsang National University, 2Soil and Fertilizer Division, National Institute of Agricultural Sciences

This study was conducted to evaluate the catalytic activity of Fe-impregnated biochar (Fe-BC) in heterogeneous Fenton oxidation (HFO) for removing azo dye orange G (OG) from solution under various conditions. The optimum molar Fe concentration for maximum catalytic activity of Fe-BC in HFO with acceptable effluent Fe concentration was found at impregnation of 0.25 M. The main factor influencing OG degradation in HFO reaction with Fe-BC was the initial H2O2 concentration, the initial Fe-BC dosage, the initial OG concentration, solution pH and temperature. The 99.7% OG and 44.2% TOC removal efficiencies were achieved within 2 hr of reaction at optimum conditions of 0.075 g/L H2O2, 0.5 g/L Fe-BC for 0.1 g/L OG at an initial pH of 5.5 under 25 oC. The catalytic activity of Fe-BC on the OG degradation in HFO was divided into the initial slow induction reaction and the subsequent rapid decomposition reaction. The evaluation for successive HFO reaction showed that Fe-BC can be use at least 4 runs and maintained above 89.3% OG removal. In addition, the concentration of Fe released from Fe-BC during the HFO reaction was below 2 mg/L, which meeting the water quality standard of effluent. Overall, application of Fe-BC in HFO for treating wastewater contained non-degradation pollutants offers more economical, safe, and recyclable advantages than conventional Fenton oxidation catalysts.

PES-6

Metabolic engineering of Clostridium acetobutylicum for the production of short-chain ester

Hyeon Jeong Seong, Hyeon Ji Noh, Yu-Sin Jang*

Institute of Agriculture & Life Science (IALS), Department of Agricultural Chemistry and Food Science Technology, Division of Applied Life Science (BK21 Plus Program),

Gyeongsang National University, Jinju, Republic of Korea

Esters are valuable chemicals that have widely used as a cosmetic, fragrance additive for foods and pharmaceutical industry. In the commercial process, esters are produce using chemical synthesis but chemical synthesis under hazardous conditions using toxic solvents and high temperature. In this study, we engineered in Clostridium acetobutylicum strain in order to produce short chain esters, efficiently. To do this, first C. acetovbutyrlicum ATCC 824, was transformed by introduction of the alcohol acyltransferase (AATs) from Malus sp. (apple). Batch culture of the engineered C. acetobutylicum strain CaAAAT expressing the apple AAAT gene produced 46.45 mg/L of short chain esters. In this study, we demonstrated the use of the engineered C. acetobutylicum for the short chain ester production. [This work was supported by a grant from the Ministry of Science and ICT (MIST) through the National Research Foundation (NRF) of Korea (NRF-2016R1D1A3B04933184).]

PES-7

Optimization of residual analysis method for flutriafol in agricultural produce using the QuEChERS method with

LC-MS/MS

Hyun Ho Noh1, Hyeyoung Kwon2*, Danbi Kim1, Chang Jo Kim1, Byeong-Chul Moon1, Jin-Ho Ro1, Min-Seok Oh1, Hyo Sub Lee1,

Sujin Baek1, Kee Sung Kyung3

1Chemical Safety Division, National Institute of Agricultural Sciences, 2Rural Development Administration, Planning and Coordination Bureau, 3Department of Environmental and

Biological Chemistry, Chungbuk National University

This study was carried out to determine analysis method for the pesticide residue of flutriafol in agricultural products, brown rice, soybean, apple, green pepper, mandarin and Kimchi cabbage, using QuEChERS method with LC-MS/MS. To establish the extraction method, samples were extracted by two methods widely used in AOAC and EN method, and the recoveries were calculated. After extraction, recovery of flutriafol in agricultural products were all in the range of 91.1-105.3%. In order to establish the purification method, recoveries were calculated after purification by adsorbents, such as PSA, C18 and GCB. As results, recoveries in those were found to be 87.8-116.3%, indicating that it is considered possible to perform two extraction methods and purification using an adsorbent. Therefore, it is considered that both extraction methods and adsorbents for purification can be used to analyzed flutriafol in agricultural products. To survey matrix effect in the MS detector, pure standard and matrix-matched standard were analyzed and then matrix effect was calculated using equation of calibration curve. As results, matrix effects were found to be low in all sample preparation methods. Therefore, flutriafol in agricultural products for the residual pesticide analysis could be analyzed by approaching QuEChERS method with LC-MS/MS which is quickly analysis method. Also, it is reasonable to use matrix-matched standard calibration because matrix effect may be different depending on sample.

Poster Presentation


Poster Presentation

PES-12

Uptake of veterinary antibiotics by plants grown in soil contaminated with veterinary antibiotics



Plants act as the natural sink for a variety of toxins in the environment, including veterinary antibiotics (VAs). The aim of this study was to evaluate the uptake of VAs by three different types of plant- 1. Root vegetable (Daucus carota, carrot) 2. Leafy vegetable (Lactuca sativa, lettuce) 3. Fruit/vegetable (Capsicum annum, pepper). Three VAs over a wide range of molecular weight was selected for the study-sulfadimethoxine (SDZ), oxytetracycline (OTC), streptomycin (STR). In this study, STR antibiotics could not be extracted from the plant samples. Being a water-soluble polar antibiotic with a large molar mass (728.69 g mol-1) STR cannot be taken up easily by plants. Also, the sandy clay loam used in the study with 30.73% clay can form strong complexes with STR. SDZ and OTC were extracted from all three plants. VAs in roots was higher than in aerial parts. In carrot, VAs was higher in the peel/skin in comparison to the flesh. Bio-concentration factor (BCF) was highest in carrot followed by lettuce and pepper. The BCF values for carrots ranged from 0.01025±0.0539 to 2.1322 ±0.0033, and 0.01818 ±0.0233 to 5.8593 ±0.0015 μg kg-1 for OTC and SDZ respectively. Similarly, the BCF values for VAs in the shoot was found to be in the order lettuce>pepper>carrot.

PES-9

Molecular and Functional study of Insect Odorant Receptor

Yong Kun Shim, Je Jun Oh, Sanung Eom, Junho Lee*


The olfactory nervous system recognizes many different chemicals in the general living environment. Insects have evolved a group of odorant-gated ion channels composed of highly-developed olfactory receptors capable of distinguishing between various chemicals with symbolic or evasive specificities. Recently, aphid genomes related to olfaction have been identified and olfactory receptors have been reported that are differentially differentiated from Drosophila. A representative receptor, odorant-gated ion channels comprised of a highly conserved co-receptor (Orco) has a homotetramer channel structure with four subunits arranged symmetrically around the central hole. It has a very similar structure to the 7-transmembrane receptor present in the human body and gating mechanism to receptors of neurotransmitters.In this study, whole cell voltage clamp recording was performed with cell expression system of OR85b gene, which is a subtype of olfactory neuro-receptor isolated from Drosophila. After the successful expression of this receptor, microbial culture extract of microorganism, a harmful insect inducer, was used to investigate whether olfactory receptor activity was regulated. The activity of the receptor was confirmed in the recording media diluted 10,000 times(microbial culture extract).Therefore, it is possible to identify attractant or repellent substance using the olfactory receptor activity regulating system of insects. Through this study, MZ01 shows the attracting phenomenon by activating insect receptor OR85b. The results of the scientific analysis of the performance of the extracts are presented.

PES-10

Effect of organic fertilizer on relative efficiency ratio of watermelon and chemical properties of plastic film house soils

with different electrical conductivity

Myung Sook Kim*, Seong-Jin Park, Seong-Heon Kim, Hyun-Young Hwang

Soil and Fertilizer Division, National Institute of Agricultural Sciences

In farmhouse, over application of organic fertilizer caused salt accumulation in plastic film house soils of watermelon. This study was investigated the effect of organic fertilizer application on relative efficiency ratio of nitrogen and soil chemical properties for watermelon cultivation in plastic film house. The soil samples were taken the plastic film house fields with different electrical conductivity (EC). The five organic fertilizers were applied at 100% level of the basis amount of nitrogen fertilizer in soil testing recommendation. The yield of watermelon in organic fertilizer was similar to NPK treatments. The relative efficiency ratio of nitrogen of watermelon tended to decrease according to increasing of EC of soil. The nitrate nitrogen and electrical conductivity showed to be slightly higher than NPK treatment. These results suggest that organic fertilizer should applied considering EC of plastic film house soils to prevent nutrient accumulation of soil and to increase the yield.

PES-11

Isolation and characterization for plant growth promoting bacteria from seaweed treated soil

Jeong Won Seo*, Yeon Jong Koo

Department of Agricultural Chemistry, Chonnam National University

The alginate degrading bacteria were expected to aggregate the soil around the roots and to participate in salt stress resistance of the plant. In this study, more than 30 strains were selected from the alginate containing medium and all isolates were identified by 16S rRNA sequencing. Stenotrophomonas pavanii and Bacillus marisflavi showed the strongest alginate degrading activity among the isolates. In addition, S. pavanii had a plant growth promoting effect and the growth promoting effect was amplified by adding alginic acid to the soil. These results suggest that S. pavanii and alginic acid can be used as promising Biofertilizers.


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PES-16

Effect of sulfadimethoxine, streptomycin and oxytetracycline on the tolerance index and free proline content of plants



This study aimed to evaluate the effect of three commonly used VA’s on the tolerance index (TI) and free proline concentration in three different plants: root vegetable (Daucus carota), leafy vegetable (Lactuca sativa), and fruit (Capsicum annum). The observed TI (%) values showed a relative difference depending upon both the type of VAs and the plant used. TI for all three plants decreased in a dose-dependent manner. Comparatively pepper plants exhibited the highest tolerance to all three VAs (SDZ: 56-63; OTC: 76-79; STR: 88-96%). On the other hand, carrot and lettuce plants were found to be sensitive to VAs. Among the three VAs the TI for all three plants, relative to control, was found to be the least affected by STR. Biochemical analysis of the roots and leaves showed a variation in accumulation of free proline. Free proline concentration increased with increasing abiotic stress (VAs concentration). Proline content in roots was slightly higher than in leaves. The highest proline accumulation was observed in the roots of carrot plants at 10 mg kg-1 SDZ (189.84±7.23 μg g-1) and the lowest in carrot leaves of control plants (21.286±4.75 μg g-1).

PES-13

Observed mycorrhizal frequency of plants grown in soil treated with veterinary antibiotics



The objective of this study was to assess the effect of three veterinary antibiotics (VA’s): sulfadimethoxine (SDZ), streptomycin sulfate (STR), oxytetracycline (OTC) on the mycorrhizal frequecy (F%) of plants grown in VAs contaminated soil. The three different vegetable plants selected were- 1. Daucus carota (carrot, root vegetable) 2. Lactuca sativa (lettuce, leafy vegetable) 3. Capsicum annum (pepper, fruit). We observed a steady decline in the ability of the VAs treated roots to form symbiotic relationship, with increasing concentration. Since, roots are the first plant organs to come in contact with the VAs the physiology of the root affected by VAs treatment also affected the intensity of mycorrhizal colonization. Among the three plants used F% was the highest in lettuce (10.14-32.37%) plants followed by pepper (9.52-22.12%) and finally carrot (5.73-29.59%). Upon comparison with control mycorrhizal frequency was affected the most by SDZ antibiotics followed by OTC and lastly STR antibiotics. The relatively higher bio-availability of SDZ antibiotics makes for their easy absorption by plant roots. A comparatively poor root development, as a result of VAs treatment, failed to sustain proper symbiotic relationship as it hampers the organic exudates released from the root.

PES-14

Risk assessment of sulfadimethoxine, streptomycin, and oxytetracycline in three plant types based on

germination index



Depending upon the nature of the veterinary antibiotics (VA’s) and plant growth conditions VA’s may pose a low-high risk to the plants. In this study, germination index of Daucus carota, Capsicum anum, and Lactuca sativa were used for the ecological risk assessment of VAs according to The European Union System for the Evaluation of Substances (EUSES). Three commonly used VA’s- sulfadimethoxine (SDZ), streptomycin (STR), oxytetracycline (OTC) with varying molecular weights were used. Carrot seeds were the most susceptible to VAs, and SDZ resulted in the highest toxicity. STR antibiotics were the least toxic with EC50 values of 60.24, 36.89 and 306.84 mg kg-1 in lettuce, carrot, and pepper, respectively. VAs toxicity to plant seed germination was in the order SDZ >OTC >STR and Carrot > Lettuce>Pepper. The difference between the lowest (SDZ treatment, carrot plant: 7.49 mg kg-1) and the highest (STR treatment, pepper plants: 306.84 mg kg-1) EC50 values was very high (approx 40.96 fold). Based on the estimated risk quotient (RQ) values: FL, AMX, and OTC were classified to pose high, medium, and low acute risk in the environment, respectively. The result indicates that SDZ antibiotics posed a high acute ecological risk in the administered soils, while OTC is estimated to have medium acute risk in the environment. STR antibiotics showed a low acute ecological risk.

PES-15

Activity of detoxification phase I and phase II enzymes in plants grown in veterinary antibiotics contaminated soil



The detoxification mechanism of plants under veterinary antibiotics (VA’s) stress was observed using - 1) Daucus carota (root vegetable), 2) Lactuca sativa (leafy vegetable), 3) Capsicum annum (fruit). Detoxifying phase I (NADPH p-450 reductase) and phase II (glutathione-s-transferase (GST)) enzymes were analyzed for the plants grown in sulfadimethoxine (SDZ), streptomycin (STR), and oxytetracycline (OTC). STR antibiotic could not be extracted and we were unable to detect any metabolites of SDZ and OTC. This may be because- a) Metabolites not formed during the experimental period. b) Metabolites formed processed by the plant detoxification system. c) Metabolites formed beyond the detection limit. NADPH p-450 reductase enzyme activity increased with increasing concentration of VAs, irrespective of the VAs and plant used. The increased enzyme activity suggests the onset of a detoxification mechanism likely responsible for the elimination of SDZ and OTC metabolites. In contrast, GST enzyme activity showed a progressive decline with increasing concentrations of SDZ and OTC in carrot and lettuce plants. However, in the case of STR, there was no significant difference from control. VAs stress had a negligible effect on GST activity of pepper plants. The highest GST activity was recorded in pepper plants at STR 10 mg kg-1 (0.00631±0.000097 nmol g-1 min-1).

Poster Presentation


Poster Presentation

PES-20

Correlation Analysis of Odor Indicators to estimate Odor Strength in Pig Farm

Saem-Ee Woo*, Gwanggon Jo, Si Young Seo, Taehwan Ha, Yu Na Jang, Min Woong Jung, Hung-Hoon Kwag, Deug-Woo Han


In Korea, livestock industry has been growing continuously as a demand from domestic meat market expand. However, this trend also caused an elevated incidents of odor complaint from neighbors. Many experts regard this as the nuisance threatening the future of the industry.To understand livestock odor, many studies have been conducted. So far, it is clear that several volatile amines and acids are responsible. Although it is crucial to recognize the odor strength, in general, people continuously exposed to odor, loose the sense of smell quickly. This means that workers in pig farm can not smell the odor and do not understand the need of abation. In this study, we attempted to quantitate odor compounds and select one which correlate most with dilution olfactometry. Several pig farms are visited regularly and air samples are collected from several spots, including inside hog barn. Air samples were subjected to quantitative analysis using techniques such as gas chromatography and air dilution olfactometer. Data were subjected to Pearson’s correlation to find out an interrelationship among factors. Statistical results suggest that dilution olfactometer data correlated more significantly with hydrogen sulfate (r=0.67), skatole (r=0.61), and phenol (r=0.59) in the air collected inside hog barn, indicating hydrogen sulphate can be a best one. This research was funded by the Rural Development Administration (Project No. PJ0135772019) Republic of Korea.

PES-17

The fate of 15N-labelled Organic Materials applied to Chinese Cabbages Cropping System

Cho-Rong Lee*, Yura Oh, Nan-Hee An, Jung-Lai Cho, Sang-Min Lee

Organic Agriculture Division, Department of Agricultural Environment, National Institute of Agricultural Sciences, Rural Development Administration

Chemical nitrogen fertilizer such as ammonium sulfate can release most nitrogen for one cropping season while, organic materials such as green manure, compost etc. are known as slow releasing their nitrogen for several months. These differences of release properties could impact on plant and soil. The aim of this study was to investigate the nitrogen fate of organic materials to plant, soil, and environment using nitrogen stable isotope (15N) as the nitrogen tracer. From September to December 2018, we cultivated chinese cabbages in 1/2000a wagner pots after application of 15N-labelled organic materials (green manure (GM), livestock compost (LC), green manure+livestock compost (GM+LC), and oil cake (OC)) and 15N-labelled chemical fertilizer (ammonium sulfate (CF)) at a rate 45.6 kg N·10a-1. The amount of 15N remaining in plant and soil was investigated at harvest. The results are summarized as follows, cumulative nitrogen mineralization rate from organic materials were lower than CF by 3 weeks after application despite of the same nitrogen input. At harvest, proportions of nitrogen uptakes by chinese cabbages were lower in organic materials than CF. While proportions of nitrogen residuals in soil were higher in organic materials than CF. Proportions of nitrogen loss into environment were not significantly different among CF, GM, OC. As results, the amount of nitrogen uptake in organic materials treatments were approximately 1/2 of CF.

PES-18

Effect of Controlled Release Fertilizers on Young Oil Palm Trees in Nursery

Yong-Sang Lee1,2*, Hoon Kim3

1Life Science Institute, Enbio. Co., Ltd., 37-20, Beonyoungro-28beon gil, Gunpo-si 15880, Republic of Korea, 2Research and Development Division, PT. Tunas Sawa Erma, Jalan Garuda Spadem No. 2B, Merauke, Papua 99611, Indonesia, 3Resources Management

Division, Korindo, Jalan MT. Haryono Kav.62, Jakarta 12780, Indonesia

Palm oil, which is the world’s most production-efficient and one of the world’s most produced and consumed oils, is produced mostly by palm plantation system in Indonesia and Malaysia. It is reported that Palm plantation area in Indonesia reaches 1.1 million hectares by 2016 and palm cultivation technology for palm oil production is continuously developed by national organizations and oil palm plantation companies, and so on. The main raw materials should be input to oil palm trees to acquire oil palm fruit bunches are mainly chemical fertilizers and some recycled oil palm by-products. Oil palm fruit bunches are harvested once per 10~15 days for 25 years until re-planting new oil palm trees, so it is absolutely necessary to select the most appropriate young palm trees in the nursery stage. During the 1-year nursery, a conventionally developed fertilizing system and Controlled release fertilizers containing N, P, K and Mg were applied and studied in Papua province, Indonesia. The fertilizer deficiency symptoms and typical abnormal growths of young oil palm trees were compared. The controlled release fertilizers were also proved to be applicable for nursery management by providing good nutrition to young oil palm trees.

PES-19

Removal of Odorous Compounds from Livestock Facility using Alkaline Liquid Spray

Saem-Ee Woo*, Gwanggon Jo, Si Young Seo, Taehwan Ha, Yu Na Jang, Min Woong Jung, Jung-Hoon Kwag, Deug-Woo Han


Volatile fatty acids (VFAs) are acidic molecules known as major contributors for manure-originated malodor. Since alkaline liquids often used as washing liquid to erase adhesive acidic compounds in industries, we hypothesized that alkaline liquid can also be effective to wash out acidic VFAs from the surface of livestock facility. To verify the hypothesis, we evaluated a capability of alkaline liquid to wash out acidic VFAs. Alkaline liquid generated with electrolysis was diluted to pH 10. A manure-odor generating system, consisted of a manure container to volatilize odor molecules, a concrete wall as adsorbent for odorous molecules, and air-flow regulator was assembled as a set. After several days of incubation, liquids, either alkaline or neutral pH were sprayed on the concrete surface. As control, few concrete were not sprayed at all. Subsequently, odorous compounds remained on the concrete were wiped with cotton swab and analyzed with TD-GC-FID for quantification. As results, VFAs on surface of spayed concrete were significantly decreased when compared with non-sprayed concrete. In case of neural pH spay, acetic acid, propionic acid, and iso-valeric acid, remained on concrete were decreased by 52%, 34%, and 41% respectively. In case of alkaline pH spray, acetic acid, and iso-valeric acid were decreased by 49%, and 24%, while, propionic acid was increased by 12%. This research was funded by the Rural Development Administration (Project No. PJ0135772019) Republic of Korea.


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PES-24

Evaluation of Food Waste Compost on Crop Growth and Soil Properties

Seong Heon Kim, Hyun Young Hwang, Myung Sook Kim, Seong Jin Park*


In Korea, the production of food waste increases with the population and industrialization; more than 10 thousand tons of food wastes were daily generated. The composting of food waste is recommended to reduce environmental problems and improve utilization efficiency of resource as bio-fertilizer. Therefore, the aim of study was to evaluate of food waste compost (FWC) on crop growth and soil. This study is conducted as follows; 1) evaluate possibility of FWC application by comparing with livestock compost, 2) determine application rate of FWC in Chinese cabbage cultivation. In experiment 1, treatments consist of control, inorganic fertilizer (N-P2O5-K2O : 320-78-198 kg ha-1), cow (CM), chicken (CH), pig (PM) and food waste (FW) compost (compost application rate: 20 Mg ha-1). In results, yield was NPK>CM>FW>PM>CH. In soil, Exch.Na content was increased slightly in PM and FW treatment. In experiment 2, FWC applications consist of a gradient of five levels (0, 10, 20, 40, 60 Mg ha-1). In results, yield was decreased when 40 Mg ha-1 applied. pH, EC and TC were not significantly different. But Exch. Na was increased when application rate was increased. As a result, FWC could be a good fertilizer, but abuse of FWC caused about salt accumulation in soil. And 40 Mg ha-1 can be proposed as optimum application rate of FWC proposed from this study.

PES-21

Assesmment of surface ditch irrigation for upland-crops in a reclaimed tidelands

Hui Soo Jun1, Jae Kwon Son2, Jae Do Song2, Jae Young Cho1*1Department of Bioenvironmental Chemistry, Chonbuk National University, Jeonju,

Republic of Korea, 2Department of Agricultural Engineering, Chonbuk National University, Jeonju, Republic of Korea

Recently there has been a growing need to convert rice paddy fields to upland producing economic crops. A various agricultural technology has been considered each agricultural circumstances in reclaimed tidelands. The effects of surface ditch irrigation in reclaimed tidelands were evaluated under different salt concentrations. The aim of the study was to design an efficient irrigation technique for cultivating field crops according to the salinity level. A randomized block design was used and the salt concentration in each treatment was adjusted to 2 dS m-1, 3 dS m-1 and 4 dS m-1 respectively. The experimental crops were maize and soybean, and an experimental area was 1 ha. For soybean crop, concentrations of total-N and total-P in soils have not been constant tendency but altered according to growth stage of crops. Exchangeable cations (Ca2+, Mg2+ and K+) decreased slowly with the growth stage of crops, however, exchangeable sodium declined sharply. Exchangeable conductivity in soils has declined in rate by one-third at the crop harvest age. By harvest time, rate of exchangeable conductivity declined by one-third. For maize cultivation, chemical changes in the soil were similar to soybean cultivation and electrical conductivity declined in rate by half.

PES-22

Effects of open channel irrigation on upland-crops in a reclaimed land



Cultivation of economic crops in reclaimed arable land requires efficient water management in accordance with soil property and weather condition. Therefore, desalinization is a priority for the efficient utilization in reclaimed tidelands. By applying water management with desalinization, reclaimed arable lands are expected to convert to economic crops cultivation. This study was conducted in randomized blocks which were adjusted to 2 dS m-1, 3 dS m-1 and 4 dS m-1 respectively. Soybean and maize were cultivated in an experimental area of 0.5 ha. Concentrations of total-N and total-P were found to alteration which is related with growth stage. With the progression of time, exchangeable cations (Ca2+, Mg2+ and K+) declined slowly and exchangeable sodium has decreased sharply with the progression. Electrical conductivity decreased by one-third.

PES-23

Improvement of soil physical properties in reclaimed land using soil conditioner



The aim of this study was to develop a soil conditioner for improvement of soil physical properties in reclaimed tidelands. Deterioration of soil physical properties is caused to dispersed soil by sodium ions in reclaimed tideland. This causes waterways and canals to collapse. Organic matter is known to contribute improvement of soil physical properties. However, readily available agricultural products such as rice straw and husks are underutilized due to their increased use for other purposes. Substitute organic matter which is easy to obtain in Korea is seaweed. Seaweeds have a large amount of sodium alginate that increases adhesion, which facilitates soil aggregation. In this study, the soil conditioner was prepared in pellet-shaped by mixing 80% pulverized seaweed, 15% sodium alginate and 5% arabia gum. The result of the treating agent, in reclaimed tideland the water stability increased by 73%.

Poster Presentation


Poster Presentation

PES-28

Application of Biochar for the reduction of odor during the livestock manure composting process

Hui Soo Jun1, Tae Kwon Park2, Jae Young Cho1*1Department of Bioenvironmental Chemistry, Chonbuk National University, Jeonju,

Republic of Korea, 2Yougiind. Co., LTD

By using Biochar that can reduce ammonia emissions during the composting process. In addition, the use of Biochar in the composting process can create a low-carbon society by reducing carbon dioxide, which is the cause of global warming. In this study, different amounts of Biochar and rice husk were adjusted with 50% cow manure and chicken manure 10%, respectively. The concentrations of released ammonia, carbon dioxide, hydrogen sulfide, and methane were measured during composing process. The results showed that Biochar was the concentration of ammonia and carbon dioxide. And, hydrogen sulfide and methane gas were detected below of the detection limit by using biochar in composting process.

PES-25

Desalinization of salt-affected reclaimed land using detergent to substitute exchangeable cations



The high concentration of exchangeable is caused to degenerate the property of soil in salt affected soil. Desalinization is priority so that converting paddy soil in reclaimed tideland. In this study, an environment-friendly substitute detergent derived from ammonium which minimizes the use of decontamination water and exchanges ions with high efficiency has been suggested. Ammonia water and acetic acid were mixed in an equivalent ratio and the pH adjusted to 7. The treatments were inundated by either water or detergent for 7 days and then drained subsequently. Consequently, following the use of cation exchange detergent, the volume of water and time required for desalinization was reduced by more than 1,500 ton/ha while and 2 years, respectively. The electrical conductivity of soil was also reduced by about 83% from 27.5 dS/m to 3.8 dS/m, furthermore, the percentages of exchangeable sodium declined by about 63% from 45.3% to 16.9%.

PES-26

Assessment of water washing method for desalinization in reclaimed tideland



Exchangeable salts in reclaimed soil have high concentrations of exchangeable sodium ions. Exchangeable sodium ions increase soil alkalinity resulting in the crumbling of soil particles, and an increase in the osmotic pressure of the soil solution. This study evaluated the effects of desalinization by water washing process. Soil with 3 different types of textures were used for the study- silt loam, sandy loam, and loamy sand. The water washing method was carried out at very high salinity (16 dS m-1) which was adjusted at the rate of NaCl:MgCl2:CaCl2 =3:1:1. Water washing method resulted in a change in pH from 7.5 to 8.5. Not only the electrical conductivity was reduced by 55% but also the percentage of exchangeable sodium decreased by 30%.

PES-27

Effect of biochar type on nitrous oxide emissions in upland soil

Sun Il Lee*, Gun Yeob Kim, Eun Jung Choi, Jong Sik Lee

Department of Climate Change & Agroecology, National Institute of Agricultural Sciences

Soil improvement with biochar has recently been proposed as a means to mitigate climate change through reducing soil greenhouse gas (GHG) emissions. The objective of this study was to the effects of three types of biochar on nitrous oxide (N2O) emissions from upland soil in the short term. A 57-days incubation study was carried out, in which three types of biochar made from the pyrolysis of rice hull, soybean straw, and pear branch. Then, the biochars were added at two levels of 5 and 10 Mg ha-1, respectively. Soil moisture content was adjusted to 70% of the water holding capacity at a temperature of 25℃. N2O emissions were analyzed 2 day, 3 day, and weekly. The cumulative N2O emissions were 216.6 mg m-2

for control, 123.7~193.1 mg m-2 for ricehull biochar treatement, 153.4~169.6 mg m-2 for soybean biochar treatment and 169.4~208.3 mg m-2 for pear branch biochar treatment. The cumulative N2O emissions were significantly decreased with increasing the biochar input amount. It was found that N2O emissions were related to the biochar type. Consequently the biochar made by agricultural residue could decrease the soil N2O emission. To verify the conclusions of this study, long-term studies in the field with crops should be conducted.


238

PES-32

Phytoremediation of hazardous chemical residues from animal burial sites



The capability of phytoremediation application on veterinary pharmaceuticals (VPs) such as acetaminophen (AM), sulfamethazine (SM), and tetracycline (TC) were investigated. The experiment was carried out in glass tanks for 2 weeks using aquatic species as Pistia stratiotes (water lettuce) and Myriophyllum aquaticum (parrot feather), and hairy root cultures of Helianthus annuus (sunflower). Water lettuce, parrot feathers, and sunflower plants were found to effective for phytoremediation of veterinary pharmaceuticals.

PES-29

The effects of soil-treated herbicides on Sticky chickweed (Cerastium glomeratum)

Chang-Wook Park, Seok-Min Lee, Hye-Bin Park, Yu-Na Kim, Hee-Soon Park, Ko-Eun Lee, Eun-Jung Park, In-Jung Lee*

Division of Plant Biosciences, Kyungpook National University, Daegu 41566, Korea

Due to the increase in international trade, the flow of exotic plants is increasing. Some plant species have a great impact on the domestic ecosystem and agricultural environment due to their excellent ability to adapt to environment such as a large number of seeds and high germination rate.Sticky chickweed (Cerastium glomeratum) is a Caryophyllales Caryophyllaceae biennial plant. It is estimated that seeds were brought with imported agricultural products from Europe in the 1980s to 2000s. It is an dominant weed that is often found in wayside and farmland.This experiment was conducted to investigate the effects of soil-treated herbicides on Sticky chickweed and to use them as basic data for developing effective management methods.The germination characteristics of the Sticky chickweed treated with herbicide in the same sowing depth were investigated. In addition, we investigated the growth and physiological activity of Sticky chickweed treated with herbicides by comparing chlorophyll contents, shoot length, and dry weight of germinated plants.As a result, the germination rate decreased in the overall treatments and the growth of some germinated treatments decreased. Therefore, it will be able to use herbicides for controlling Sticky chickweed pre-emergence.

PES-30

Monitoring of veterinary pharmaceuticals, chemical deodorants, and disinfectant



This study examined residual concentrations of agriculture chemical residues such as disinfectants (citric acid, sodium hypochlorite), chemical deodorants (amine acid, chlorine), and veterinary pharmaceuticals (acetaminophen, sulfamethazine, and tetracycline) in arable land near the livestock manure treatment facility. In this study, acetaminophen and tetracycline were below the detection limit. The concentration of sulfamethazine in soils was 4.78 to 5.48 μg kg-1. The disinfectants and chemical deodorants were detected natural background levels.

PES-31

Persistence, photodecomposition and decomposition mechanism of agricultural chemical residues in water



Secondary environmental pollution from residual agriculture chemicals in the leachates of livestock burial sites is increasing. In this study, we investigate the residual concentration, photolysis, and decomposition mechanism of veterinary pharmaceuticals (VPs)- acetaminophen, sulfamethazine, and tetracycline in water. This experiment used Rose bengal as a photo sensitizer. In the case of control, VPs decreased by the level of half-life after 14 days. Consequently, the level of the residual concentration of VPs was declined rapidly by the treatment of Rose bengal. Therefore, the residual concentration of VPs in water is not considered to be a concern.

Poster Presentation


Poster Presentation

PES-36

Residual Characteristics and Processing Factors of Cyantraniliprole and Its Metabolite in Cinidium Rhizome

Dong Kyu Jeong, Dong Yeol Lee*

Research and Development Team, Gyeongnam Oriental Anti-Aging Institute

This study was carried out to investigate the residual characteristics of insecticide cyantraniliprole and its metabolite IN-J9Z38 in medicinal herb Cinidium Rhizome and calculate processing factors by drying. The test insecticide was sprayed two times each at different spraying intervals, and were used for the analyzed after harvesting and drying process. Cyantraniliprole and IN-J9Z38 residues in Cnidium Rhizome root were extracted with QuEChERS extract method and analyzed with LC-MS/MS. The method limit of detections in both dried and fresh samples of cyantraniliprole and IN-J9Z38 were 0.004 mg/L and 0.04 mg/L, respectively. Average recoveries of cyantraniliprole among the dried and fresh Cnidium Rhizome were 89.2%-97.5% and 99.7%-104.1%, respectively, and for IN-J9Z38, 77.7%-93.1% and 75.3%-84.2%, respectively. Processing factors of cyantraniliprole in fresh Cnidium Rhizome by drying were found to be from 3.84, indicating that the cyantraniliprole residues in dried samples increased from three to four times by dring process.

PES-33

Development of QuEChERS based method for the simultaneous determination of four sulfonylurea herbicides in cabbage and spinach by liquid chromatography tandem-

mass spectrometry

Mohammad Mydul Islam Abul Kasem1, Byeong-Chul Moon1, Jin-Ho Ro1, Danbi Kim1, Sujin Baek1, Min-Seok Oh1, Hyeyoung Kwon2*

1Department of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration, 2Planning and Coordination Bureau,

Rural Development Administration

QuEChERS (quick, easy, cheap, effective, rugged and safe) method was modified to determine four sulfonylurea herbicides (bensulfuron-methyl, halosulfuron-methyl, nicosulfuron and thifensulfuron-methyl) in cabbage and spinach using liquid chromatography tandem-mass spectrometry (LC-MS/MS). Sample (10 g) extraction was carried out by 1% formic acid in acetonitrile (10 ml) solution followed by addition of 4 g magnesium sulfate and 1 g sodium chloride. Different amount of high surface area graphitized carbon black (HSGCB) and C18 was varied for cleanup. The optimized amount was obtained by 0.005 g HSGCB and 0.25 g C18 for each ml of extracts. After clean up the extract was diluted for 10 times before injection. The method was validated at spikes level of 0.01, 0.05, 0.10, 0.25 and 0.5 mg/kg. Mean recoveries range were 85% to 115%. The calibration curve correlation coefficient (R2) was 0.99 or higher, and limit of quantifications was from 0.0001 to 0.0003 mg/kg for all herbicides. Using the modified method, the herbicides exhibited low matrix effect (ME %) ≤20%, and the colour of the extracts were almost disappeared. The method showed better performance than the QuEChERS method. Thus, the modified method has promising capacity for sulfonylurea residue analysis, which could prove useful in sample preparation of cabbage and spinach, and other matrices in future.

PES-34


Jehyeong Yeon1, Ae Ran Park1, Nan Hee Yu1, Cuong Viet Han1, Ho Myeong Kim2, Hae Woong Park2, Jin-Cheol Kim1*

1Department of Agricultural Chemistry, Institute of Enviromentally Friendly Agriculture, College of Agriculture and Life Science, Chonnam National University, Gwangju 61186,

Republic of Korea, 2R&D Division, World Institute of Kimchi, Gwangju 61755, Republic of Korea

Organic acids released during the decomposition of organic soil amendments have been used in crop fields as benign chemicals to control phytopathogens. However, the use of organic acids against root-knot nematodes (RKNs, Meloidogyne spp.) has not been recommended in practice, owing to their short retention time in the soil. In this study, we tested the nematicidal activity of organic acids including maleic, fumaric, acetic, acrylic, caprylic, oxalic, and formic acids, as well as copper sulfate against Meloidogyne incognita. Maleic acid showed the strongest nematicidal activity with induction of 100% second-stage juvenile (J2) mortality and 70.47% hatching inhibition at a concentration of 0.37 mmol/L. In addition, the combination of maleic acid and copper sulfate was revealed to have an additive effect in J2 mortality. Application of a wettable powder-type formulation of a mixture of maleic acid and copper sulfate (MIX-WP30) reduced RKN disease on tomato by 51.72% in a pot experiment, suppressed gall formation on melon and decreased nematode population density in the soil. Under field conditions, the efficacy of MIX-WP30 (46.73%) was comparable with that of the commercial nematicide fosthiazate (48.38%). This study identified the effectiveness of MIX-WP30 against RKNs, and demonstrated that there was an in vivo synergistic effect of MIX-WP30. Based on its nematicidal activity, MIX-WP30 could be used as an effective nematicide for RKN management.

PES-35

Study on Residual Properties of Cyazofamid and Its Metabolite in Herbal Medicine Cinidium Rhizome

Dong Yeol Lee*, Dong Kyu Jeong, Won Min Jeong, Young Sook Kil, Seung Mi Sin, Young Min Goo, Sang Gon Kim


This study was carried out to elucidate residual properties of fungicide cyazofamid and its metabolite CCIM in herbal medicine Cinidium Rhizome and calculate processing factors by drying. The test fungicide was treated three times each at different spraying intervals, and were used for the analyzed after harvesting and drying process. Cyazofamid and CCIM residues in dried and fresh in Cnidium Rhizome were extracted with QuEChERS EN method and analyzed with Liquid chromatography - tandom mass spectrometry. The method detection limits in both dried and fresh samples of cyazofamid and CCIM were 0.004 mg/L and 0.02 mg/L, respectively. Average recoveries of cyazofamid among the dried and fresh Cnidium Rhizome were 95.0%-95.5% and 97.6%-100.9%, respectively, and for CCIM, 97.7%-104.7% and 83.6%-100.7%, respectively. Processing factors of cyazofamid in fresh Cnidium Rhizome by drying were found to be from 6.80, indicating that the cyazofamid residues in dried samples increased from six to seven times by drying process.


240

PES-40

Risk Assessment of Operator Exposure During Treatment of Fungicide Fluazinam on Korean Cabbage Plantation

Il Kyu Cho*, Young Gon Oh, Dong-Gi Lee, Jae Ung Seol, Ji Myung Kim

Research Center for Environmentally Friendly Agricultural Life Sciences, Jeonnam Bioindustry Foundation

Dermal and inhalation exposure of agricultural operators to fluazinam WP (50.0%) have been studied during pesticide mixing/loading and power sprayer application (10 replicates, each of 1,000 L of spray suspension) on the ten of Korean cabbage farms located in Goesan of the Republic of Korea. Outer clothing, inner clothing, gauze, nitrile gloves and an IOM (Institute of Occupational Medicine) sampler were used to measure the potential exposure for applicators in compliance with whole body dosimetry (WBD) exposure protocol. All mean field fortification recoveries were between 77.1% and 103.7% (CV=8.7%) at the level of 100 LOQ while LOQ for fluazinam was 0.005 μg/mL using LC-QTRAP-MS/MS. The major exposure to fluazinam appeared in all part of the body during application. The daily exposure amounts of ADE (Actual Dermal Exposure) and AIE (Actual Inhalation Exposure) for mixer/loader were 160.71 and 0.30 μg, respectively, whereas the amounts of ADE and AIE for applicator were 96,040 and 4.45 μg. The total exposure on agricultural applicators showed at the level of 0.4304 mg~590.4983 mg to fluazinam during treatment for Korean cabbage. The TER (Toxicology/Exposure Ratio) showed 39.2 (>1) when AOEL of fluazinam (0.0035 mg/kg bw/day) was used as a reference dose for the purpose of risk assessment of the application.

PES-37

Aroclor Accelerates the Toxicity of POPs in Activity of Mitochondrial complex III from Tail of Zebrafish

Songhee Lee1, Eun Ko2, Sooim Shin1,2*1Department of Bioengineering and Biotechnology, College of Engineering, Chonnam National University, Gwangju 61186, Republic of Korea, 2Interdisciplinary Program of

Bioenergy and Biomaterials Graduate School (BK21 Plus Program), Department of Bioengineering and Biotechnology, Chonnam National University, Gwangju 61186,

Republic of Korea

Persistent organic pollutants (POPs) are organic compounds resistant to environmental degradation causing problems in human health. Aroclor, a polychlorinated biphenyl, is a kind of POPs that interrupt endocrine system. We proceed experiments to know how these toxins influence in metabolic pathway of zebrafish model system which is often used to study human genetics and diseases. Two group of experiments were compared for the study; one is exposed to 5 kinds of POPs mixture (4,4’-DDT, chlordane, Heptachlor, beta-HCH, Hexachlorobenzene), and the other is exposed to POPs mixture with an aroclor. The zebra fish raised in continuous water system with flowing different POPs+aroclor concentrations for 3 months. After isolating the mitochondria from the tails of each zebrafish group, the activities of the mitochondrial electron transport chain complex I to IV were measured spectrophotometrically and compared them according to POPs concentration. As a result, mitochondrial complex III of female zebrafish in aroclor treated group decreased much more its activity in concentration dependent manner other than POPs only treated group. On the other hand, the complex III of the male zebrafish with aroclor treated group exhibited similar activity to all other groups. These results demonstrate that aroclor boosts up a toxicity of POPs on endocrine system depending on sex and it specifically inhibits activity of mitochondrial complex III in muscle of zebrafish.

PES-38

Determination of analysis method for the pesticide residue of chlormequat with LC-MS/MS in paprika and potato

Sujin Baek1, Min-Seok Oh1*, Hyeyoung Kwon2, Hyun Ho Noh1, A. K. M. Mydul Islam1, Danbi Kim1, Jin-Ho Ro1, Byeong-Chul Moon1

1Chemical Safety Division, National Institute of Agricultural Sciences, 2Planning and Coordination Bureau, Rural Development Agency

This study was performed to compare extraction method of chlormequat that it is used as a plant growth regulator to inhibit cell elongation in paprika and potato. In order to extract chlormequat, it was used to methanol and acetonitrile. In methanol extraction method, samples were extracted with 1% formic acid in methanol of 10 mL and then those were centrifuged at 3,500 rpm for 5 min. Another method, acetonitrile extraction, was used with QuEChERS method called by EN method. The samples were extracted by cetonitrile of 10 mL and then those were centrifuged at 3,500 rpm for 5 min after put in EN extraction kit, 4 g MgSO4, 1 g NaCl, 1 g Na citrate, 0.5 g disodium citrate sesquihydrate. The chlormequat in paprika and potato was analyzed with LC-MS/MS using ESI positive mode for improvement sensitivity and selectivity. As results, the LOQ of chlormequat in paprika and potato was 0.01 mg/kg and linear equation of calibration curve was over 0.99 in all samples. The recovery of chlormequat extracted with acetonitrile method found to be 70-120 (RSD 10%) indicating that this method is suitable for residual pesticide analysis of chlormequat. However, methanol extraction method was not. Therefore, extraction solvent should be used acetonitrile with EN QuEChERS extraction kit for residual pesticide analysis of chlormequat in paprika and potato.

PES-39

Leaf chlorophyll measurement by SPAD meter for estimation of leaf nitrogen content in ‘Gamhong’/M.9 apple orchard

Seong Eun Lee*, In Bog Lee, Pyoung Ho Yi, Gopal Selvakumar

Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, RDA

The SPAD is a non-destructive portable meter measuring leaf greenness. There have been many attempts to use SPAD value for leaf nitrogen (N) estimation in various crops because positive linear relationship has been demonstrated between SPAD readings and total extracted chlorophyll by many studies. Still, there isn’t enough information on relationships between leaf N and chlorophyll content in apple orchard. This study aimed to assess whether there are seasonal changes in correlation coefficient between SPAD value, as a measured chlorophyll content, and N content of apple leaves, and to confirm the possibility of practical use of SPAD meter for nutrient diagnosis in ‘Gamhong’/M.9 apple orchard. The leaf N and chlorophyll content of ‘Gamhong’ apple tree were measured in fourty commercial apple orchards by chemical analysis and SPAD-502 chlorophyll meter. In this study, leaf N content and SPAD value exhibited positive correlation from May to August. So, measuring SPAD value of apple leaves can be an inexpensive, efficient, and convenient way to estimate leaf N content for successful N management of apple orchard, although there are some influences of environmental factors, such as different soil properties and meteorological condition of a certain region. Meanwhile, the measurement is recommended to be conducted in July (r=0.81***) or August (r=0.88***) because the correlation was much stronger at that time than in May (r=0.63***) or June (r=0.61***).

Poster Presentation


Poster Presentation

PES-44

Effects of Biochar and Effective Microorganisms on Ammonia Gas Emission from Upland Soil with Urea for

Soybean Cultivation

Su-Lim Lee1, Jae-Hoon Lee1, Ju-Hyeon Lee1, Jong-Hwan Park2, Dong-Cheol Seo1,2*

1Department of Agricultural Chemistry and Food Science & Technology, Gyeongsang National University, 2Division of Applied Life Science (BK21 Plus) &

Institute of Agriculture and Life Science, Gyeongsang National University

This study was carried out to evaluate the ammonia gas emission characteristics under commercial effective microorganisms (C-EM) and biochar (BC) treatments in soybean cultivation soil (SCS) with urea. Ammonia gas emissions were assessed by an acrylic pot, which included 50 g of SCS and different levels of urea (0.5-2.0 g/pot), BC (0.1-2.5 g/pot) and C-EM (0.05-5.00 mL/pot). The ammonia gas emitted from the pot was collected in a dilute sulfuric acid solution (0.05N) and analyzed by the indophenol method. Ammonia gas emissions from all treatments except the control were highest at 2 days, while it decreased gradually after 2 days. Ammonia gas emissions from SCS increased as the urea concentration increasing. On the other hand, ammonia gas emissions in SCS with C-EM and BC decreased compared to SCS with only urea, which significantly decreased with increasing C-EM and BC treatment levels. Overall, both C-EM and BC were effective in reducing ammonia gas emissions from SCS with urea. However, this study investigates only the effects of C-EM and BC on ammonia gas emission characteristics in SCS, so it need to evaluate soil characteristics and crop growth according to their treatment in future.

PES-41

Molecular responses to phosphine and ethyl formate in two developmental stages of two-spotted spider mite

(Tetranychus urticae)



Phosphine and ethyl formate are effective against various insect pests such as two-spotted spider mite (Tetranychus urticae). In order to understand the mode of fumigation action of phosphine and ethyl formate, we investigated the molecular responses in the developmental stages of T. urticae against the fumigants. Cytochrome c oxidase (COX) was similarly inhibited in nymphs and adults. In the case of alpha-naphthyl acetate esterase (ANAE) and glutathione-S-transferase (GST), the responses after exposure of each fumigant were significantly different in nymph and adult stage. ANAE was the most inhibited enzyme by phosphine in mite adults. In the case of GST, it was found that the activity increased by two fumigants in mite nymphs, while the adult was not affected. In regard to gene expression, the expression of cox11 gene was significantly reduced by 6 to 8 times in T. urticae exposed to ethyl formate. On the other hand, nymphs responded sensitively when compared with adults after exposure to phosphine. The expression level of 6 genes increased by 1.5 to 8 times compared to control. Taken together, similar biological responses were observed for ethyl formate and phosphine in T. urticae, but it was observed to be more influenced by phosphine in nymph. This study suggests that different control methods seem to need according to developmental stages of T. urticae.

PES-42

Novel resistance mechanisms in the rice weevil, Sitophilus oryzae to phosphine

Kyeongnam Kim, Hwang-Ju Jeon, Sung-Eun Lee*


Phosphine (PH3) resistance in stored-product insect pests has been reported worldwide, including Korea, where Sitophilus oryzae has developed resistance to PH3. Here, two PH3-resistant S. oryzae strains such as moderate resistant (MR) and strong resistant (R), were used to find the development of PH3 resistance. The values of Ct99 towards MR and R strains were approximately 3- and 30-fold higher than that for the control strain (S), respectively. Cytochrome c oxidase (COX), a potential PH3-target site, increased and the Lineweaver-Burk plots of the EF-inhibited COX activities indicated an uncompetitive mode of inhibition for the S strain and noncompetitive modes for the resistant strains. The RT-qPCR analysis showed significant up-regulation of cat, wnt7, wnt11, and gh48 and down-regulation of jhi, casp, and gh31 in the R strain. The expressions of the fatty acid biosynthesis enzymes, acetyl-CoA c arboxylase and fatty acid synthase, and energy metabolism enzymes, COX2 and succinate dehydrogenase were significantly down-regulated in both resistant strains. The next-generation sequencing of the mitochondrial DNA revealed missense mutations in nad4, nad5, and nad6 and point mutations in cox1 and cox2. To our knowledge, this is the first report of the mutations related to PH3 resistance. Taken together, S. oryzae resistance to PH3 was definitely acquired by the overall transformation of energy metabolism to overcome PH3 toxicity.

PES-43

Residual Evaluation of Insecticide Bifenthrin Sprayed on Radishes (Raphanus sativus var. Sativus)

Young-Jin Hong1, Won-Bae Joh1, Seon-Yeong Im2, Ji-Hye Kim2, Byung-Hun Song2, Dong-Jin Lee1,2*

1Department of Crop Science & Biotechnology, Dankook University, Cheonan-si 31116, Republic of Korea, 2DICA, Cheonan-si 31116, Republic of Korea

This study was carried out to investigate residue pattern of insecticide bifenthrin sprayed on radish (Raphanus sativus var. Sativus), one of minor crops in Korea, and to utilize it as basic data for safety evaluation of residues. The tested variety of radish used in experiment was Kwonnongyeoleumaltarimu. Bifenthrin is a pyrethroid insecticide known as comparative persistent pesticide in soil environment. The formulated bifenthrin used in test was 2% a.i. wettable powder and diluted with water to 1,000 times. The diluted bifenthrin were spayed 2 times on the foliage of radish growing in green house. The test plots were designed by the application days of pesticide before harvest, they were 30-21, 21-14, 14-7 and 7-0 days. The quantitative analysis of bifenthrin residue in radishes was performed using a HPLC/PDA (UV 210 nm). The limits of quantification (LOQ) of leaves and roots were 0.003 and 0.002 mg/kg, respectively. The recovery tests were performed at two fortification levels (0.1 and 0.5 mg/kg), the rates were 90.5~117.7% (CV =7.49 %) in leaves and 85.1~104.0% (CV=4.31%) in roots. The residue amounts of bifenthrin in leaves and roots were ranged 0.01~0.37 mg/kg and 0.01~0.05 mg/kg, respectively. The residual amount gradually were decreased with elapsed time after application of the pesticide. Based on the results, it was suggested the safe use guideline and MRL of bifenthrin on radish.


242

PES-48

Deposition and metal content of particulate matter on the surface of shrub and tree leaves

Seon Ju Kwon, Seung Ju Cha, Joo Kyung Lee, Jin Hee Park*

Chungbuk National University, Agricultural Chemistry

It is known that different plant species have ability to deposit different amount of particulate matter on their leaves. Therefore, particulate matter on different plant species was fractionated according to the particle size (PM , PM , PM ) and quantified. Metal distribution on the leaves and metal concentration of particulate matter of different size fraction was analyzed using micro X-ray fluorescence (μ-XRF) and inductively coupled plasma atomic emission spectroscopy (ICP-AES), respectively. Heavy metals contained in fine dust could affect the growth of plants if accumulated on the leaves. In this study, the mass of PM deposited on the surface of the shrub leaves ranged from 0.0181 μg/cm² to 13.1 μg/cm², while PM ranged from 0.00213 μg/cm² to 10.4 μg/cm². The mass of PM ranged from 0.00338 μg/cm² to 3.27μg/cm². Particulate matter contained cluster elements such as Al, Ca, Mg and Si and heavy metals including Cu and Zn. Cluster element concentration was higher in coarser size while heavy metal concentration was higher in finer size fraction. Particulate matter on pine tree leaves was observed under a stereoscopic microscope, and Cu and Pb distribution on the leaves was shown by μ-XRF.

PES-45

Investigation of half-lives in soil and uptake by radish of veterinary antibiotics

Song-Hee Ryu*, Hyoin Yoon, Jeewon Rhee, Hyejin Yu, Sung-Jin Lim, Geun-Hyoung Choi, Hyo-Sub Lee, Sang-Won Bark


Concerns about the environmental impact of antibiotics used in animal husbandry have increased. Because most antibiotics are excreted to livestock manure, antibiotics can be released into the environment through the use and application of manure in agriculture. Therefore, this study was conducted to assess of half-lives in soil and uptake by plant of three commonly used veterinary antibiotics, i.e. sulfamethazine (SMZ), sulfathiazole (STZ), oxytetracycline (OTC). Three antibiotics were mixed with soil and then collected to determine the remaining antibiotic ingredients. For investigation of uptake by radish of antibiotics, radish was grown on soil mixed with three antibiotics and then soil samples and leaf and root of radish were collected. As a result, half-life of OTC in soil was longer than those of SMZ and STZ. Sulfamethazine (SMZ) was detected in the radish root at level of 1 mg/kg, while OTC and STZ were not detected in the crop samples. These results suggest that SMZ has the potential to be taken up by crops.

PES-46

Monitoring of paprika internal electrical conductivity under metal stress

Seung Ju Cha1, Jin Hee Park1*, Seon Ju Kwon1, Joo Kyung Lee1, Hyun Jun Park2

1Chungbuk National University, Agricultural Chemistry, 2Soil Research Institute, Prumbio Co., Ltd.

Electrical conductivity within a plant represents the physiological activity of the plant including ion and water transport. The internal electrical conductivity (ECp) of the plant stem helps to understand the physiological changes of plants in relation to heavy metal stress. In this study, to evaluate the effects of Cr3+, Cr6+ and Mn2+ on physiological activity of the paprika, paprika was exposed to 50 mg/L and 5 mg/L of Cr3+ and Cr6+, respectively, and 100 mg/L and 50 mg/L of Mn2+ in hydroponic solution. Hydroponic solution without metals was served as a control. Two electrodes with three needles were inserted on both sides of the paprika stem, electrical resistance was logged every 1 h and electrical resistance was converted to electrical conductivity. The ECp of the paprika under metal stress was lower than that of the control indicating that water and ion transport was disrupted due to metal stress. The ECp of paprika slightly decreased with Mn2+ treatment, but it was recovered after two days of Mn exposure. External symptoms of paprika by Cr were not observed, but both Cr3+ and Cr6+ disturbed water and ion transport of the paprika. Therefore, monitoring ECp can be used to diagnose physiological changes in plants under environmental stress conditions before symptoms appear.

PES-47

Effect of pyroligneous acid on amidase activity in soil

Joo Kyung Lee1, Hyun Jun Park2, Seung Ju Cha1, Seon Ju Kwon1, Jin Hee Park1*1Chungbuk National University, Agricultural Chemistry,

2Soil Research Institute, Prumbio Co., Ltd.

Urea is nitrogen fertilizer widely used in Korea. Urea is broken down into ammonia and carbon dioxide by a variety of urea hydrolysis enzymes in soil. Because ammonia is likely to be volatilized and lost from soil, controlled release of ammonia from urea is required. Pyroligneous acid was used to prevent rapid hydrolysis of urea by reducing soil urease activity. Pyroligneous acid inhibited the activity of microbial and plant urease, thus preventing rapid dissolution of ammonium from urea. Amidase is an enzyme that catalyzes the hydrolysis of an amide, which includes glutaminase and asparaginase. In this study, the effect of pyrolignous acid on the amidase activity was evaluated. Upland soil was amended with urease, glutamine and aspargine, pyrolignous acid was added and soil was incubated for 24 h. After incubation of the soil, the NH4

+-N content was analyzed using the indophenol blue method. The enzyme activity was estimated based on the content of released NH4

+-N. The result showed that pyroligneous acid inhibited the activity of soil amidase. Pyroligneous acid inhibited C-N bond cleavage resulting from similar inhibition mechanism of urease, glutaminase and asparaginase.

Poster Presentation


Poster Presentation

PES-52

Determination of Optimal Conditions for the CO2 and NH3 Treatment for the Induction of the Infective Juveniles of

Entomopathogenic Nematodes

Sang Heyong Park1, Kwang-Hyun Baek2*, Yeon Su Yu1*1Research Division, Daedong Tech Co., Gyeongsan, Gyeongbuk 38401, Republic of Korea, 2Department of Biotechnology, Yeungnam University,

Gyeongsan, Gyeongbuk 38541, Republic of Korea

The solid culture system for the entomopathogenic nematode (EPN) isolated in Korea can be industrialized by switching the stage of EPN to the infective juveniles (IJ). The conversion to IJ at the end of solid culture system is very important considering the insecticidal efficacy and shelf storage period. To determine whether the various CO2 and NH3 treatment could affect the induction rate of IJ at the end of the solid culture system, several combinations varying the concentrations and treatment time were attempted and measured the efficacy for IJ induction. We induced IJ with the highest efficiency with the exposure time for the CO2 treatment for 5 minutes and NH3 for 3 minutes and with the treatment date as 9 days after EPN inoculation. The optimum concentrations for both CO2

and NH3 were 80~90% for the induction of EPN IJ. Based on these results, the optimum conditions for inducing IJ can be adjusted, and be adapted to industrial production system of EPN by the solid culture system.

PES-49

Residual Patterns of Dithianon and Flufenoxuron in Lettuce as a Minor Crop

Tae Hyun Lee, Kyu-Won Hwang, Jeong Hoon Sun, Hyun Jin Kim, Kyung Su Jung, Joon-Kwan Moon*

Department of Plant Life and Environmental Sciences, Hankyong National University, Anseong 17579, Republic of Korea

This study was carried out to investigate the residual characteristics of dithianon and flufenoxuron on lettuce. The pesticides were sprayed 2 or 3 times onto the crop at a week interval prior to harvest, and the samples of lettuce were collected at 0, 1, 3, 5, and 7 days after the final application of pesticides. Samples clean-up with SPE-florisil, and residues were analyzed by HPLC-UVD. Limit of Quantitation (LOQ) were both 0.05 mg/kg, recovery rates of dithianon at two fortification levels of 0.5 and 2.5 mg/kg were determined 78.3~80.3% and 73.0~73.3%, and flufenoxuron were 83.9~92.0% and 91.6~92.4%, respectively. The residue levels of dithianon and flufenoxuron were 47.51, 7.38 mg/kg at 0 DAT (day after treatment) and decreased to 14.04, 2.57 mg/kg at 7 DAT, respectively. The residual amounts of pesticides interpreted using first order kinetics model show that dissipation constants of dithianon and flufenoxuron in lettuce were 0.1324 day-1 and 0.1428 day-1, respectively. In addition, the biological half-life in lettuce was 5.2 days for dithianon and 4.9 days for flufenoxuron.

PES-50

Spatial Distribution of Soil Chemical Properties Using Geostatistics

Woo Ri Go*, Yeon Kyu Sonn, Sang Ho Jeon, Byung Keun Hyun, Byung Hwan Seo, Sun Gang Yun

Soil and Fertilizer Division, National Institute of Agricultural Science, RDA

In this study, spatial distribution mapping of pH and organic matter content in rice field was produced for essential soil information to crop growth prediction model using unmanned aerial vehicle (UAV). The soil sampling was lattice type, in 10 m intervals divided into topsoil and subsoil in rice field in Gimje area. Spatial distribution mapping was prepared for soil pH and organic matter content using analysis data. After checking the coordinates of longitude and latitude of the sampling area using the GPS, we created sampling point in the land cadastral map using the ArcGIS program. Soil chemical properties of non-survey area were estimated by geostatistical methods. And then spatial distribution characteristics were confirmed by using semivariogram. Results of semivariogram of topsoil and subsoil pH nugget were 0.52 and 0.39, range were 19.9 and 38.3, respectively. And topsoil and subsoil organic mater nugget were 0.00 and 0.14, range were 19.9 and 19.9, respectively. Model type of pH and organic mater were gaussian. Spatial distribution can be used to compare the chemical content of soils visually, and if the accumulation of multi-year data is possible, it will be possible to check the change of soil in the land parcel unit. Through this, I think that obtain of reliable data on the soil among the environmental factors affecting the crop.

PES-51

Evaluation of Ammonium Ion Behavior for Obtaining Information on Secondary Particulate Matter Originating from

Various Soils

Se-Wook Hwang1, Jong-Hwan Park1, Jeong-Woo Lee2, Dong-Cheol Seo1,2*1Division of Applied Life Science (BK21 Plus) & Institute of Agriculture and Life Science,

Gyeongsang National University, 2Department of Agricultural Chemistry and Food Science & Technology, Gyeongsang National University

Ammonia gas emitted from soil is one of the main source of secondary particulate matter, which is attracting attention from many researchers worldwide. Especially, the behavior of ammonium ions (NH4

+) in soil is closely related to the ammonia gas emission. Therefore, this study was preferentially conducted to evaluate the adsorption behavior of NH4

+ in three different soils [Greenhouse soil (GS), Paddy soil (PS), and Forest soil (FS)]. Maximum adsorption capacity of NH4

+ was higher in the order of PS>FS≥GS, and the amount of NH4

+ converted to NO3- in all the soils was negligible. The adsorption amount of NH4

+ in the soil without organic matter (OM) was decreased to 1-5% compared to the soil with OM, indicating that the adsorption NH4

+ in the soil is closely related to OM. The release concentration of cations in the soil was increased during adsorption reaction of NH4

+, suggesting that the exchange mechanism between soil cation and NH4

+ had a dominant influence on the adsorption behavior. Ammonium ions adsorbed on the soil was the most released by HCl solution compared to other solutions (KCl, H2O and NaCl). The results of the FTIR demonstrate that the adsorption of NH4

+ in the soil is affected by carboxyl and hydroxyl groups in the OM.


244

PES-56

Carbon storage capacity of paddy soil with organic cultivation

Yangsoo Han*, Choong-Bae Park, Hongshik Nam, Kwang-Lai Park, Kee-Choon Park

Organic Agriculture Division, Dept. of Agricultural Environment, NAAS, RDA

Carbon (C) storage in agricultural soils is considered to be an important factor mitigating global climate change. Although paddy soil represents a large portion of cropland in South Korea, little information on the potential C storage is available and the efficiency of C storage in paddy soil might differ among various cultivation systems (i.e., organic cultivation and conventional cultivation). In this study, topsoil (0-15 cm) carbon storage capacity was quantified from five regions in South Korea having different soil properties and cultivation systems: Suncheon, Uiseong, Buyeo (A and B) and Gimhae. Topsoil carbon storage capacities of organic paddy soil were 35.8±4.8 Mg ha-1 in Suncheon, 46.4±2.5 Mg ha-1 in Uiseong, 34.2±8.1 Mg ha-1 in Buyeo A, 30.5±4.6 Mg ha-1 in Buyeo B, and 26.6±6.3 Mg ha-1 in Gimhae. On the other hands, Topsoil carbon storage capacities of conventional paddy soil were 29.2±1.4 Mg ha-1 in Suncheon, 36.2±13.2 Mg ha-1 in Uiseong, 19.9±2.0 Mg ha-1 in Buyeo A, 12.9±2.8 Mg ha-1 in Buyeo B, and 25.8±3.2 Mg ha-1 in Gimhae. As a result, a significant differences between organic and conventional cultivation in carbon storage for paddy topsoils were observed. In addition, the differences were further increased with the addition of green manure in the organic cultivation.

PES-53

An abalone-derived bacteria has strong fucoidan metabolic activity and compete with plant to use soil nutrients

Yeji Gil, Heejeong Oh, So Hee Yang, Euyeon Kim, Yeonjong Koo*

Department of Agricultural & Biological Chemistry, Chonnam National University

Managing soil microbe is an important technique in the eco-friendly farming. We isolated an marine bacteria from abalone and discovered plant bacterial interaction in the soil environment. Only one bacteria (AF1) was isolated from abalone and AF1 had strong metabolic activity to the fucoidan, which is a sulfated polysaccharide found in various species of brown algae and brown seaweed. In the growth comparison with various carbon source, AF1 had higher affinity to the fucoidan than to the alginate which is another major polysaccharide of brown algae. To test plant growth stimulation effect of AF1, we inoculated AF1 into the soil of net melon. AF1 didn’t have growth stimulation effect but reduced the plant growth enhancing effect of fucoidan itself, unexpectedly. This result shows that some marine bacteria may compete with plant to consume carbon source in the soil without any positive plant growth effects. By the way, some isolated soil bacteria F2 and F8 had plant growth stimulation effect even they also had strong fucoidan metabolic activity. These results shows that the different plant-bacterial interaction come from bacterial native environments.

PES-54

Chemical characteristics of groundwater based on different cropping types in Gyeongnam Province, South Korea

Jae Young Heo1*, Hyeon Ji Cho1, Young Han Lee1, Si Lim Choi1, So Jin Yeob2

1Environmental Agricultural Research Division, Gyeongsangnam-do Agricultural Research and Extension Services, 2Climate Change and Agroecology Division,

National Institute of Agricultural Sciences

This study was conducted to obtain basic data of the chemical characteristics of groundwater based on different cropping types in Gyeongnam Province, South Korea. Groundwater sampling sites from paddy field 5, upland 5, and controlled horticultural field 10 sites were collected on April and July in every years from 2016 to 2018. As a result of comparing the average of three years water quality analysis, pH was no significant change in paddy field, upland and controlled horticultural field by 6.7~7.2. EC was no significant change in annual, but land to use were in the order of controlled horticultural field > upland > paddy field and its contents were 0.43, 0.33, and 0.25 dS m-1, respectively. The NO3-N contents by land use were in the order of upland > controlled horticultural field > paddy field and its contents were 9.65, 8.90, and 4.83 mg L-1, respectively. In addition, the c oncentrations of Cl- were significantly higher in controlled horticultural field. Also, EC, Cl-, Ca2+, K+, and Mg+ contents were highest in controlled horticultural field types agricultural ground water in Gyeongnam Province, South Korea. But, heavy metals were mostly not detected. In conclusion, groundwater chemistry is heavily influenced by land use. These results indicated that they are traceable to agricultural non point sources fertilizers, livestock farms and sewage.

PES-55

Comparison of Cadmium Retain Capacities in Microplastic Polyethylene and Polypropylene

Ju Hyun Eom1, Dong Cheol Seo1,2*1Department of Agricultural Chemistry and Food Science & Technology,

Gyeongsang National University, 2Division of Applied Life Science (BK21 Plus) & Institute of Agriculture and Life Science, Gyeongsang National University

The vast amount of plastic waste emitted into the environment and the increasing concern of potential harm to wildlife has made microplastic pollution a growing environmental concern. In recent years, studies on microplastics have focused on their decomposition in the ocean. However, our knowledge is limited about the relationship between microplastic and metal ions in freshwater. Therefore, this study was conducted to evaluate the retain capacity of Cd by microplastics [polyethylene (PE) and polypropylene (PP)] in freshwater. The retain capacity of Cd by PE was higher than that of PP according to results obtained from Langmuir and Freundlich adsorption models, indicating that PE has a higher affinity for Cd than PP. Specifically, retain capacity of Cd by PP was not observed in low Cd concentration, but that of PP at high Cd concentration is relatively increased compared to low Cd concentration. These results indicate that retain capacities of Cd by microplastics are dominantly influenced by metal ion concentration. Our study found that microplastics in freshwater are related to the behavior of Cd. However, in order to clarify the specific relationship between Cd and other metals, additional research (kinetic and mechanism) should be carried out.

Poster Presentation


Poster Presentation

PES-60

Non-negative matrix factorization for impurity profiling of seized methamphetamine

Beom Jun Ko1, Dong Won Shin1, Jae Chul Cheong1, Wonho Lee2, Suhkmann Kim2, Jin Young Kim1*

1Forensic Genetics & Chemistry Division, Supreme Prosecutors’ Office, Seoul 06590, Republic of Korea, 2Department of Chemistry, Center for Proteome Biophysics and Chemistry Institute for Functional Materials, Pusan National University,

Busan 46241, Republic of Korea

Methamphetamine impurity profiling is a powerful forensic tool which is combined of instrumental and statistical analysis in methamphetamine trafficking investigation. It gives useful information about starting materials, synthetic method and distribution routes of drug. Recently seized methamphetamine samples are having fewer impurities and lower amount of impurities and these cause difficulties in extracting forensic information from given samples. As the number and amount of impurities are reduced, a new strategy to overcome recent developments in illegal manufacturing and purifying techniques for methamphetamine is needed for impurity profiling.To cope with this problem, non-negative matrix factorization (NMF) was suggested for calculating virtual impurities not detected by instruments. For calculating virtual impurities, a test sample set comprised of samples believed to be synthesized by Emde method were used and these sample set were used for comparing the differences in predicted values to actual ones. As the numbers of samples in test set were increased, the predicted values became closer to actual values detected by instruments.This study is applicable to highly purified methamphetamine samples and should provide additional forensic information to law enforcement agencies.

PES-57

Analysis of the distribution of assimilation products and the characteristics of transcriptomes in rice by

submergence during the ripening stage

Hyeonseok Lee*

National Institute of Crop Science, Crop Production & Physiology

Research on the submergence stress of rice has concentrated on the quiescence strategy to survive in long-term flooding conditions based on Submergence-1A (SUB1A). In the case of the ripening period, it is important that submergence stress can affect the quality as well as the survival of rice. Therefore, it is essential to understand the changes in the distribution of assimilation products in grain and ripening characteristics in submergence stress conditions. We confirmed that the distribution rate of assimilation products in grain was decreased by submergence treatment. To understand this phenomenon at the molecular level, we analyzed the relative expression levels of genes related to sucrose metabolism, and found that the sucrose phosphate synthase gene was decreased in the seeds and leaves, but not in the stems. Furthermore, the sucrose transporter gene decreased in the seeds and leaves, but increased in stems. We also analyzed the biological metabolic processes using the KEGG mapper with selected differentially expressed genes (DEGs). We found that the expression of genes for each step related to starch and D-glucose synthesis was down-regulated in the seeds and leaves but up-regulated in the stem. The results of this study provide basic data for the development of varieties and corresponding technologies adapted to submergence conditions, through understanding the action network of the elements that change in the submergence condition.

PES-58

Threshold concentration of arsenic in paddy soils causing changes in nutrient availability to rice plant

Myeong-Hui Kim, Bu-Ung Choi, Ji-Hyun Yoon, Oh-Do Kwon, Ji-Hyock Yoo*


Pot experiment was conducted under flooded soil condition to estimate the threshold concentration of arsenic in soil that affects the availability of nutrients. Total 6 levels of As contaminated soils were prepared from As spiking. Soil solutions were sampled at growth stages after flooding for analysis of major nutrients such as N, P and correlation analysis with arsenic concentration in soil was performed. The growth of rice plant was inhibited at arsenic concentration over 75 mg/kg for loam soil and 58 mg/kg for sandy loam soil. The arsenic threshold concentration in soil was estimated to be in the range of 62-75 mg/kg from the functional equation among soil As, NH4-N and PO4-P concentrations in soil solution. P contents in shoots and roots were not affected by the soil arsenic concentration. However, N, K and Ca contents in shoots form the high arsenic soil (exceeding 89 and 106 mg/kg for loam and sandy loam soil, respectively) decreased significantly. In the case of roots nutrient contents (N, K and Ca) from the high arsenic soil (exceeding 52 and 75 mg/kg for loam and sandy loam soil, respectively) decreased significantly. The results show that the condition exceeding threshold soil arsenic concentration (62-75 mg/kg) inhibits rice growth and nutrient consumption by rice plant (excepting P) and consequently decreases the nutrient contents in rice plant.

PES-59

Changes of Soil Organic Carbon stock and GHGs emission from LULUCF in South Korea (1990 to 2017)

Seong Jin Park*


Greenhouse gases (GHGs) emission is estimated and reported by a guideline book from Intergovernmental Panel on Climate Change (IPCC). Tier 1 methodology by IPCC use IPCC default factors and Tier 2 uses country specific emission factor. In this research, we conducted to calculate the greenhouse gases emission from 1990 to 2014 by IPCC Tier 1 methodology. According to the guide line, there are five sectors causing GHGs emissions and one of the land-use, land-use change, and forestry (LULUCF) sector deal with greenhouse gases (GHGs) emission resulting from land-use such as agricultural activities and land-use change. GHGs emission in this sector consists of carbon stock changes in soil, N2O emission from disturbance associated with land-use conversion to cropland, CO2 emission from agricultural lime application. The emission by carbon stock changes conversion to cropland and lime application was 3592.44, 32, 125 Gg CO2-eq, respectively. Total emission from the LULUCF-Cropland sector in 2017 was 4,582 Gg CO2-eq. They increased by 508% since 1990 and decreased by 6% compared to 2017. Total emission from this sector shows that the largest sink is the soil carbon loss and its increase trend in total emission in recent years is largely due to loss of cropland area.


246

PES-64

Lipid profiles of Tetranychus urticae adults after exposure to ethyl formate and phosphine


School of Applied Bioscience, Kyungpook National University

Methyl bromide, which had been used for quarantine of pests around the world, is banned due to global warming. Therefore, phosphine and ethyl formate have been introduced to the market as alternatives for this substance. They have shown strong insecticidal effects in eradicating the nymphs and adults of Tetranychus urticae. Interestingly, their insecticidal activity is resulted from the inhibition on the activity of cytochrome C oxidase, leading to disturb energy production through the inhibition on electron transport system. However, the effect of these substances on lipid biosynthesis has not been studied. Therefore, this study investigates how the pattern of lipid production of the pest is changed after the fumigation of these two substances by using MALDI-TOF MS/MS profiling. Phosphine and ethyl formate treatments resulted in a total of 82 lipid production changes, which were statistically significant. Among them, the structure of 19 lipids was identified, and PI (38:6) increased about 2.56 times in relation to phosphine treatment, but no significant change was observed in ethyl formate-treated T. urticae. It was also confirmed that PI (34:0) decreased 2.55 times in the insect after the ethyl formate treatment, and 8.19 times in relation to phosphine treatment. Based on these results, it is expected that these two fumigants affect the activity of various proteins involved in lipid biosynthesis of the insect pests.

PES-61

Long-term effects of soil amelioration methods on metal polluted farmlands

Buung Choi1, Ji-Hyock Yoo1*, Oh Do Kwon1, Jin Woong Park1, Ji Hyun Yoon1, Seon Mi Lee1, Goo-Bok Jung2

1Chemical Safety Division, Department of Agro-Food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea, 2Soil and Fertilizer Division, Department of Agricultural Environment, National Institute of

Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea

This study was conducted to compare long-term effects of soil amelioration on metal polluted farmlands among several physiochemical methods. Amelioration methods include soil covering(A), soil covering +steel slag(B1), soil covering +limestone(B2), soil covering+steel slag+limestone(C) and soil covering+steel slag+limestone+others(D). Soil samples were collected from the farmlands ameliorated more than 6 years ago nearby abandoned metal mine. Total As, Cd and Pb in soils were extracted with aqua regia and bioavailable metal fractions were extracted with 1M NH4NO3, 0.01M CaCl2 and 0.43N HNO3. Total As, Cd and Pb in soils were 2.6~70.6 mg/kg, 0.1~4.1 mg/kg and 14.4~248.1 mg/kg, respectively. The 1M NH4NO3 extractable contents were N.D.~5.5 mg/kg (As), N.D.~7.9 mg/kg (Cd) and N.D.~24.1 mg/kg (Pb). The 0.01M CaCl2 extractable contents were N.D.~0.1 mg/kg (As), N.D.~0.3 mg/kg (Cd) and N.D.~0.6 mg/kg (Pb), and 0.43N HNO3 extractable contents were N.D.~7.2 mg/kg (As), 0.02~3.0 mg/kg (Cd) and 1.3~161.9 mg/kg (Pb). For each method, the order of ratios of bioavailable contents extracted with 1M NH4NO3 to total contents were B2 (0.2%)<B1 (0.3%)<C (0.4%)<A (0.5%)<D (1.6%) for As, B1 (2.1%)<B2 (6.6%)<C (11.6%)<A (17.5%)<D (25.2%) for Cd and B1 (< <0.03%) <B2 (0.03%)<A (0.5%)<C (0.8%)<D (1.4%) for Pb, respectively. In case of 0.01M CaCl2 extraction, the ratios for B1 method were 0.03%, 0.3% and 0.01% for As, Cd and Pb, respectively. The results indicated that B1,”soil covering+steel slag” method might be optimum for metal immobilization in soils for these surveyed area.

PES-62

Chemical properties of soils from ameliorated farmlands near closed metal mine

Se In Kim, Ji Hyock Yoo*, Myung Hee Kim*, Seon Mi Lee*

Dep. of Agro-Food Safety, National Institute of Agricultural

After 6-10 years from the completion of soil amelioration, the chemical properties of soils from ameliorated fields were analyzed to assess whether soils are optimum for cultivation. Soil organic matter content was analyzed by Tyurin method and available phosphorus was analyzed by Bray NO.1 method. The pH ranged from 4.9 to 7.7 showing higher value than optimum range of farmlands (5.5-6.5), probably because of the effect of limestone used for amelioration. Available phosphorus content in soils were very low (1.3-673 mg kg-1, mean 58.4 mg kg-1) in comparison with the optimum content (300-500 mg kg-1), so phosphorus fertilization is necessary. Exchangeable Ca and Mg contents were 1.5-16.4 and 0.5-6.3 cmol+ kg-1, respectively and there were many fields exceeding optimum range (5.0-6.0 for Ca and 1.5-2.0 cmol+ kg-1 for Mg). This is also considered to be the effect of ameliorant, steel slag and limestone. Soil organic matter contents were less than 20 g kg-1 in many fields comparing with optimum range (20-30 g kg-1), so organic matter contents should be increased. The results probably reflect the long-term effects of soil ameliorant on the soil properties and it was recommended for many fields to enrich the soil.

PES-63

Arsenic Removal Utilizing Biogenic Lepidocrocite Reductively Formed by Clostridium Sp. Strain C8

Chang Hu Kang, Hor-Gil Hur*, Youri Yang, Jisu Lee, Yongseok Ko

School of Earth Sciences and Environmental Engineering, Gwangju Institute of Science and Technology (GIST), 123 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea

Arsenic (As) is a ubiquitous and highly toxic oxyanion-forming metalloid that extensively exists in various natural environments. There have been a few studies for removal of arsenic using iron (oxyhydr)oxides. Here, we investigated that adsorption and structural incorporation of arsenite or arsenate in the presence of ferrihydrite by Clostridium sp. Strain C8 which isolated from methane gas producing consortium. Results of XRD, SEM and ferrozine assay showed that biogenic lepidocrocite (γ-FeOOH) was produced through a low-level iron reduction process when arsenic is present, whereas hematite (Fe2O3) was produced when arsenic is absent. It is assumed that negatively charged arsenate (H2AsO4

- and HAsO42-)

or neutral species arsenite (H3AsO3) is adsorbed on iron oxide and inhibited bacterial iron reduction for a long time and transforming into another type of iron oxide in anoxic condition. Result of sequential chemical extraction indicate that the ratio of arsenic adsorbed on the lepidocrocite structure surface was decreased and the incorporation rate was increased with incubation time as a result of the biogenic lepidocrocite formation. Therefore, transformation of ferrihydrite to biogenic lepidocrocite under the influence of arsenic can be a solution to removal of arsenic by incorporating arsenic and plays an important role in the cycling of arsenic in the environment.

Poster Presentation


Poster Presentation

PES-68

Impact of different types of manure on the compost quality and greenhouse gas emissions during food waste composting

Hyun Young Hwang, Seong Jin Park*

Division of Soil and Fertilizer, National Institute of Agricultural Sciences

To overcome the problems of difficult decomposition of food waste (FW) and long composting period, various combination materials are used in the composting process. However, each additive has different effects on the raw material transformation, compost quality and emissions of ammonia and greenhouse gases (GHGs). This study was investigated the effect of adding different types of manure to the FW composting process. Apart from a control treatment (Food waste only, FO), three treatments were conducted with the livestock manure (LM): (1) mixing cow manure (FC); (2) mixing swine manure (FS); and (3) mixing poultry manure (FP). The composting experiment was conducted in 52L composting chamber for 105 days. The results showed that composting with LM reduced nitrogen loss by NH3 and N2O emission. Most of NH3 emission was observed within one month after composting in FO, other treatments didn’t show clear patterns. FC and FS significantly increased CO2 emission by 76% over the FO rather, slightly reduced CO2 flux was observed in FP. The patterns of CO2 were similar with temperature changes. The highest peak was found at initial thermophilic phase. There was no significant difference in CH4

production. Germination index was increased in FC and FS, they could accelerate maturation rate. These results suggested that FW composting with LM is recommendable to improve compost quality and control GHGs emissions.

PES-65

Monitoring of ambient ammonia in an agricultural area using diffusive passive air samplers

Ho-Young Lee1, Tien Do Van1, Min-Kyu Park1, Sang Ryong Lee2, Sung-Eun Lee3, Sung-Deuk Choi1*

1School of Urban and Environmental Engineering, Ulsan National Institute of Science and Technology (UNIST), 2Department of Agricultural Convergence, Jeonju University,

3School of Applied Biosciences, Kyungpook National University

Ammonia (NH3) is a well-known basic gas in the atmosphere, which significantly contributes to fine particle (PM2.5) formation. NH3 is emitted mainly from agricultural activities, especially livestock farming and composting livestock waste, which have received much attention as major sources of NH3 in South Korea. Diffusive passive air samplers (PAS) have been widely used in the monitoring of ambient NH3 because of their advantages such as easy installation and cost-effectiveness. In this study, the performance of PAS was verified, and the monitoring using PAS was conducted in an agricultural area with farmlands and cattle sheds. The cartridge adsorbent containing NH4

+ was extracted using deionized water and analyzed with an ion chromatograph (IC). For quality assurance/quality control (QA/QC), blank samples were analyzed and the PAS were deployed in duplicate. Moreover, linearity between sampling periods and concentrations was evaluated to ensure the maximum adsorbent capacity of PAS. The sampling periods were varied from 2 to 28 days, and the linearity of NH4

+ uptake during the sampling period was satisfactory (R2 >0.95). After the verification of PAS, real samples were deployed for two weeks at a farmland and nearby cattle sheds. The levels of NH3 at 11 sites in the agricultural area were compared with those in rural areas, and the spatial distribution was investigated.

PES-66

Land-use Management for Sustainable Rice Production in Reclaimed Coastal Tideland Soils: A review

Hyun-Jin Park1, Hye-In Yang1, Se-In Park1, Bo-Seong Seo1, Dong-Hwan Lee1, Young-Jae Jeong1, Ju-Yeon Lim1, Han-Yong Kim2, Woo-Jung Choi1*

1Department of Rural and Biosystems Engineering, Chonnam National University, 2Department of Applied Plant Science, Chonnam National University

The properties of secondary salt-affected soils developed from improper irrigation and drainage management and their effects on rice growth and yield are well documented. However, relevant information on coastal reclaimed tideland (RTL) soils, which are classified as primary salt-affected soils developed through salt accumulated sediments is lacking. In this paper, we reviewed physical and chemical properties of RTL soils in comparison with non-RTL soils and analyzed the relationship between rice production and soil salinity in RTL to suggest agricultural management practices for sustainable rice production in RTL. In similar to secondary salt-affected soils, we found that RTL soils are characterized by high alkalinity, salinity, and sodicity, and rice yield were negatively correlated with salinity. However, it was also found that lower fertility (e.g., organic matter and phosphorus) of RTL soils than non-RTL soils might also hamper rice yield in RTL soils. Correlation between years after reclamation and soil properties of RTL showed that cultivation of rice with annual fertilization and organic inputs increased soil fertility but salinity and sodicity did not show significant pattern, suggesting that natural desalinization in RTL soils is hardly to be achieved with conventional rice cultivation. Therefore, it is suggested that not only fertilization management but also salinity management such as gypsum application, tillage, and proper irrigation may be necessary to improve rice production is RTL soils.

PES-67

Assessment of Electrical Conductivity of Saturated Soil Paste from 1:5 Soil-Water Extracts for Reclaimed Tideland Soils

in South-Western Coastal Area of Korea

Hyun-Jin Park1, Hye-In Yang1, Se-In Park1, Bo-Seong Seo1, Dong-Hwan Lee1, Young-Jae Jeong1, Ju-Yeon Lim1, Han-Yong Kim2, Woo-Jung Choi1*

1Department of Rural and Biosystems Engineering, Chonnam National University, 2Department of Applied Plant Science, Chonnam National University

Measurement of electrical conductivity of saturated soil paste (ECe) for assessment of soil salinity is time-consuming, and thus conversion of EC of 1:5 soil-water extract (EC1:5) to ECe using a dilution factor may be of help to monitor salinity of huge number of soil samples. This study was conducted to evaluate the dilution factor for reclaimed tideland (RTL) soils of South Korea. Soil samples (n=40) were collected from four RTLs, and analyzed for EC1:5, ECe, and cation compositions of 1:5 soil-water extract. The dilution factor (8.70) was estimated by regression analysis between EC1:5 and ECe, and the obtained dilution factor was validated by applying to an independent data set (n=96) of EC1:5 and ECe. The ECe measured and predicted was strongly correlated (r2=0.74, P<0.001), but ECe was overestimated by 16% particularly for the soils with high clay content and low sodium adsorption ratio (SAR). This study suggests that using the dilution factor to convert EC1:5 to ECe is feasible method to monitor changes in the soil salinity of the study RTL. However, overestimation of ECe should be cautioned for the soils with high clay content and low SAR.


248

PES-72

Vegetated ridge and sandbag may not reduce soil erosion and loss of carbon and nutrients from upland fields

Se-In Park1, Hye In Yang1, Hyun-Jin Park1, Bo-Sung Seo1, Dong-Hwan Lee1, Kwang-Sik Yoon1, Han-Yong Kim2, Woo-Jung Choi1*

1Department of Rural and Biosystems Engineering, Chonnam National University, Gwangju 61186, Republic of Korea, 2Department of Applied Plant Science,

Chonnam National University, Gwangju, 61186, Republic of Korea

Though construction of vegetated ridge (VR) and placement of sandbag (SB) across the slope of upland fields are believed to be effective in reducing soil erosion and nutrient loss, relevant data are lacking to confirm such expectations. In this study, the effects of VR and SB on loss of soils and carbon (C), nitrogen (N), and phosphorus (P) (CNP) were investigated through both artificial (in dry season) and natural (in rainy season) runoff experiments on upland fields cultivated with maize (Zea mays L.). Unlike the expectations, both VR and SB were not effective in reducing loss of soils and CNP. For VR, accelerated convergent flow caused by ridge failure, which occurred through breaching of ridge by water when water stored in furrow exceeds the water storage capacity of the ridge, seemed to lead to excessive loss of soils and CNP. For SB, the malfunction of SB could be ascribed to loss of soils and CNP through seepage water flow through gaps between SBs and between SB and soil surface. Maize growth and yield were not affected by VR and SB, coinciding the lack of the effects of VR and SB on soil and nutrient loss. As VR and SB are easy to be implemented and cost effective, however, further study is necessary to correct the flaws of VR and SB.

PES-69

Effect of soil amendments on As bioavailability in As-contaminated paddy soil

Dae-Won Kang1, Ji-Hyock Yoo2, Da-Young Kim3, Hyuk-Whan Song1, Oh-Kyung Kwon4, Won-Il Kim4*

1Food Quality and Safety Center, Korea National Food Cluster, 2Chemical Safety Division, National Institute of Agricultural Science, 3Department of Environmental Horticulture,

University of Seoul, 4O-Jeong Eco-Resilience Institute, Korea University

This study was conducted to reduce mobility and bioavailability of As in the contaminated paddy soil near abandoned mine by treating soil amendments and to suggest the appropriate management for safe agri-food production. Soil pH increased in Yesan soil treated with agri-lime (AL) and steel slag (SS) treatment, and significant increases in poultry manure (PM) and gypsum treatment were not identified. The content of DOC in soil according to the treatment of soil amendments was significantly increased at the beginning of the incubation period in soil treated with AL and SS, but it tended to decrease at gypsum treatment. In addition, DOC content in soil shows the positive correlation with plant available form of As in soil, which is estimated to have been reduced by a combination of techniques such as covalence with non-small chemical species in calcium and soil solutions supplied during gypsum treatment and reduction of As mobility due to reduction of DOC. More detailed research should be continued to take into account the efficiency as well as the effects of relevant factors such as changes in soil acidity, potential change in oxidation-reduction and physical chemistry of the soil that affect the uptake of crops, along with the transformation of the biological efficacy of soil As upon the treatment of soil amendments in the soil.

PES-70

Temperature response of soil organic matter decomposition in volcanic ash soils under agricultural land-use

Hye In Yang, Hyun-Jin Park, Se-In Park, Woo-Jung Choi*

Department of Rural & Bio-Systems Engineering, Chonnam National University, Gwangju 61186, Republic of Korea

Volcanic ash (VA) soils act as a substantial carbon (C) sink due to the unique physicochemical properties, but increases in temperature under climate change may alter the pattern of soil organic C (SOC) storage by affecting SOC mineralization. To investigate the temperature sensitivity of VA soils under agricultural land-use, Q10 of SOM decomposition (increases in SOM decomposition per 10oC rise in temperature) was compared with non-volcanic ash (NVA) soils. The soils were incubated at 15, 25, and 35°C for 30 days without external organic matter input, and for another 30 days with rice straw application. Before the straw addition, the averaged Q10 value of VA soils (1.94) was greater than that of NVA soils (1.70), reflecting higher concentration of recalcitrant SOM in VA soils which is more sensitive to temperature rise. However, after the straw addition, the Q10 values decreased to 1.40 and 1.50 for VA soils and NVA soils, respectively, indicating that rice straw decomposition was less sensitive to temperature compared to already existing SOM that were more stabilized. This study suggests that though VA soils contain greater SOC than other soils, the capacity of VA soils to store SOC may decrease with warming and thus further study is necessary to protect SOC in VA soils under increasing temperature.

PES-71

Applicability of circulating system of closed chamber and INNOVA for estimating the flux of ammonia emission

from swine manure

Min-Suk Kim1,2, In-Sun Kang1, Sang-Ryong Lee1*1Department of Agricultural Convergence, Jeonju University,

2O-Jeong Eco Resilience Institute, Korea University

This study was conducted to construct a circulating system using closed chamber and an optical sound wave analyzer on order to quickly evaluate the ammonia emission rate in livestock manure. The applicability of the system was evaluated using artificial manure and 10 types of swine manure and liquid fertilizer collected from swine farms. Volatilized ammonia in artificial manure increased with increasing pH and total ammoniacal nitrogen in solutions. In comparison with conventional ammonia analysis method, the results showed very similar tendency of ammonia emission patterns. The slurry and liquid fertilizer collected from swine farms showed weak alkalinity and varied ammoniacal nitrogen concentration and more noise than artificial manure was appeared. Conventional method of collecting and analyzing the total amount of ammonia through pump have a low possibility of ammonia loss. But in order to analyze a sample, there are reagents, manpower and time consuming difficulties. The system constructed in this study is advantageous in that it can be analyzed in a short period of time with relatively little use of additional reagents, and the results are also reliable. In addition, the chamber system is expected to be used for research such as exploration of factors influencing the ammonia generation by compost and liquid fertilizer application for agricultural soils.

Poster Presentation


Poster Presentation

PES-73

The performance of sediment trap for water pollutants and soil removal from cropland runoff

Han-Yong Kim1*, Woo-Jung Choi2, Hyeon-Jin Park2, Jae-Hyeok Park1

1Department of Applied Plant Science, Chonnam National University, 2Department of Rural and Biosystem Engineering, Chonnam National University

The erosion of soil and transport of water pollutants from cropland via rainfall runoff threaten both the soil fertility and the quality of surface waters. In environmental preservation agriculture, it is therefore essential to minimize a soil and water pollutants runoff from cropland. The objectives of this study were to assess the performance of sediment trap (i.e. detention ponds) for water pollutants and soil removal from corn land runoff. Field experiment (ca. 400 m2) was conducted with three sediment trap (L 2.0 m×W 1.0 m×D 0.5 m) treatment plots and their control plots established at downward margin of the sloped cropland (2.5%) in a randomized complete block design. During natural rainfall events, mixing of rainfall runoff between treatment plots was minimized by separating experimental plots with a 30 cm PVC barrier pushed 10cm into the soil. The sediment trap significantly reduced water pollutants like suspended solid, total nitrogen, total phosphorus and total carbon in rainfall runoff from corn land. In general, however, this effect tended to be smaller in late season as sediment deposition increases. Over the seasons, the sediment trap resulted in significant soil detention, preventing soil loss of 1.1~1.7 kg m-2 from corn land. We conclude that sediment trap can be a useful tool to realize an environmental preservation agriculture via removing soil and water pollutants from cropland runoff.

PES-74

A study of the interaction between benzalkonium chloride and albumin by spectroscopic methods

Sujin Park, Semi Kim, Moonsung Choi*


Benzalkonium chloride (BKC), also known as alkyldimethylbenzylammonium chloride, is used as antiseptics, disinfectants, preservatives in eye drop for its antimicrobial activity. Albumin, which is the most abundant protein in tears, acts as a lubricant on the ocular surface. In this study, we tried to reveal the effect of BKC on albumin. Interaction between BKC and albumin was investigated by UV-vis spectrophotometry and conformational changes were identified through intrinsic fluorescence quenching. Aggregation of albumin was appeared in the presence of high concentration of BKC by observing A340. Significant quenching of albumin was observed in the presence of BKC. As a result, we revealed that BKC interacted with albumin and induced aggregation at high concentration and also quenched the intrinsic fluorescence of albumin which could be inferred that BKC affected the conformation of albumin.

PES-75

Biotoxicity of UV photodegradation off-gas for 10 VOCs

In Sun Kang1, Min Suk Kim1, Sang Ryong Lee1, Jinying Xi2*1Department of Agricultural Convergence, Jeonju University,

2School of Environment, Tsinghua University

Many kinds of products in the off-gas after VOCs UV photodegradation have been detected. The biological toxicity of these products in the off-gas was investigated by testing the acute and genetic toxicities of water solution in which the off-gas were absorbed. 10 types of VOCs which have high removal efficiency (RE) by UV photodegradation were selected to do the toxicity tests. The results will give some indication on what is the risk level of applying UV photodegradation in VOCs gas emission control. Luminescent Bacteria, Daphnia magna, Fish and Honeybee are appointed as the experiment species and are commonly used in the standard experiment. So, Luminescent Bacteria and Daphnia magna are selected as the model organism to test the acute toxicity. Especially, one of the most common species that is frequently used is Daphnia magna, whose propagation power is particularly strong, and its sensitivity to toxic material is so outstanding, making the species particularly suitable for testing the toxicity (OECD, 2004; US. EPA, 2002; ASTM, 1992). Umu test is employed to evaluate the genotoxicity of off-gas. Umu test was developed by Oda et al. which are used to evaluate the genotoxic potential of harmful pollutants in the environment. The test is accomplished by estimate the extent of DNA-damaging agents to induce expressions of umu operon.


250

PFS-4

A Study on the Content of Heavy Metals for Other Processed Products

Yon Koung Park*, Sung Ah Park, Yung Ran Na, Hyeon Jin Kim, Hyeon Jeong Kwon, Hyun Nho Cho, Jung Mi Kang

Food Analysis Division, Busan Metropolitan City Institute of Health and Environment

We estimated the heavy metal contents in 82 food samples (pill, powder processed products). The contents of lead (Pb), cadmium (Cd), arsenic (As), copper (Cu), chromium (Cr) and nickel (Ni) were determined using an inductively coupled plasma mass spectrometry (ICP-MS). The content of mercury (Hg) was determined using a mercury analyzer (Combustion gold amalgamation method). Average concentrations of Pb, Cd, As, Cr, Ni, Cu and Hg were 0.46 mg/kg, 0.12 mg/kg, 2.21 mg/kg, 2.1 mg/kg, 1.58 mg/kg, 6.03 mg/kg and 0.01 mg/kg in the samples. The mean contents of Cu and As were high and Hg was low in the foods. The highest level contents of Pb, Cd, As, Cr, Ni, Cu and Hg in the sampels was as follow; Noni (4.2 mg/kg), Sea mustard (0.85 mg/kg), Sea tangle (30.92 mg/kg), Balloon-flower root (0.13 mg/kg), Sea tangle (34.6 mg/kg), Sea tangle (11.12 mg/kg), Assai palm (20.76 mg/kg). Levels of heavy metal in the samples detected heavy metal standard were lower than the standard limit. However, 62.6% of the examined samples was not detected heavy metal standard. It detected the heavy metal contents and need limited standard for food safety in various other processed products.

PFS Food Sciences

PFS-1

Survey on Harmful Materials of on Herbs Commonly Used for Food and Medicine in Busan

Nam Ho Kim*, Hye Kyung Cho, Jong Bae Youn, Seung Ju Lee

Drug Analysis Team, Busan Metropolitan City Institute of Health and Environment

This survey was conducted to investigate the residue amount of harmful materials (lead, cadmium, arsenic, mercury, sulfur dioxide) in 141 herbs commonly used for food and medicine for market in Busan area in 2018. Total 141 samples were contaminated with lead, cadmium, arsenic, mercury, sulfur dioxide. The lead, cadmium and arsenic were analyzed with ICP-OES, mercury was determined with a mercury analyzer and sulfur dioxide was analyzed by modified Monier-Williams method. Harmful material contents in samples were significantly different among herbs. Lead was detected from 77 samples (54.6%), cadmium was 86 samples (61.0%), arsenic was 62 samples (44.0%), mercury and sulfur dioxide were 141 (100.0%) samples. The contents of harmful materials were as follows (detected range and mean value); Lead (Pb): not detected~3.93 (0.46) mg/kg, cadmium (Cd): not detected ~0.67 (0.18) mg/kg, arsenic (As): not detected~0.68 (0.17) mg/kg, mercury (Hg): 0.0001~ 0.08 (0.005) mg/kg, sulfur dioxide (SO2): 0.6~369.1 (7.8) mg/kg respectively. The highest value of Pb was detected in Cinnamomi Ramulus (Vietnam), Cd was detected in Acanthopanacis Cortex (China), As was detected in Nelumbinis Folium (China), Hg was detected in Polyporus (China), SO2 was detected in Dioscoreae Rhizoma (China).

PFS-2

Survey on Pesticide Residues and Heavy Metals of Local Agricultural Products in Busan Area

Ji-Yoon Lee1*, Kyeong-A Kim2, Dongu-Ju Park2, Sun-Mi Lee2, Han-Nui You2, Eun-Ju Song2, Sang-Kee Min2

1Office of Eomgung Agricultural Products Inspection, Busan Metropolitan City Institute of Health and Environment, 2Office of Eomgung Agricultural Products Inspection,

Busan Metropolitan City Institute of Health and Environment

This survey was conducted to investigate the residue amounts of harmful materials on local agricultural products in Busan area. A total of 188 samples was collected from local foods market, mart, urban vegetable garden, environment-friendly agricultural product center, etc. Pesticide residues were analyzed to 130 pesticides according to multi class pesticide multiresidue methods announced on Korean Food Code using with the GC-ECD, GC-NPD, and GC-MSD. As the results, the pesticide residues were detected from 11 samples (5.9%), but their values were not over the maximum residue limits (MRLs). The detected samples were Chinese chive (36.4%), Perilla leaf (30.8%), Crown daisy (25.0%), Korean plum (20.0%), and Welsh onion (5.3%), respectively. And the kinds of detected pesticides were Chlorfenapyr (3), Tefluthrin (3), Diethofencarb (2), Chlorothalonil (1), Diniconazole (1), Fenobucarb (1), Pyridalyl (1), and Thifluzamide (1) in order.The heavy metals were analyzed with regard to Pb and Cd using ICP-OES. The mean value of Pb content was 0.019±0.041 mg/kg and Cd, 0.006±0.015 mg/kg respectively. Their measured values were shown within MRLs.

PFS-3

A Study on Pesticide Residues Monitoring of Agricultural Products Used for Food and Medicine in Busan area

Younju Ok, Sohyun Park*

Office of Banyeo Agricultural Products Inspection, Busan Metropolitan City Institute of Health and Environment

This study examined pesticide residues on agricultural products that are commonly used in both food and medicine in Busan area. Total of 126 samples (97 samples for food agriculture, 29 samples for medicinal herb) were analyzed 165 kinds of pesticides by GC/MS/MS. As the results, the pesticide residues were detected in 17 samples (13.5%). Of which were detected, 16 samples were used for food agriculture and 1 sample was used for medicinal herb. The products that exceeded their MRLs were not found. Frequently detected products were jujube (12), lycium fruit (3) and schisandra fruit (2) and these kinds of agricultures are subject to the Food Act even if they are used as medicines. A total of 16 Types of pesticides were detected such as Tebuconazole (7), Bifenthrin (6), Hexaconazole (6), Chlorothalonil (3), Cypermethrin (3), Buprofezin (2), Chlorpyrifos (2), Fludioxonil (2), Iprodione, (2) Myclobutanil (2), Diazinon (1), Difenoconazole (1), Fenpropathrin (1), Kresoxim-methyl (1) and Tebufenpyrad (1).

Poster Presentation


Poster Presentation

PFS-8

Comparison of pesticide residue in Korean melon between sample preparation method in Korea and Japan

Danbi Kim, Jin-Ho Ro*


This study compared residual amounts of mandipropamid and diflubenzuron in the Korean melon between sample preparation method in Korea (unpeeled) and Japan (peeled) . The MRL will be deleted for diflubenzuron; but for mandipropamid MRL is 0.01 mg/kg in Japan. The sample was analyzed by the QuEChERS method using HPLC-MS/MS. Mandipropamid and diflubenzuron showed normal range of recovery (70~120%) and relative standard deviation value (less than 20%) for unpeeled and peeled Korean melon. The limit of detection were 0.01 ppm for both pesticides. For pesticide application, mandipropamid and diflubenzuron were diluted 2000 times and sprayed three times and twice onto the crops, respectively at an interval of seven days with pre-harvest intervals of 3 days. The sample was collected after 0, 1, 3, 5, 7 and 14 days of last application. The samples were pre-treated with unpeeled and peeled samples, separately. The result showed that the amount of mandipropamid and diflubenzuron for unpeeled samples were 0.063~0.483 and 0.027~0.431 mg/kg, respectively, and for peeled samples were <0.01~0.039 and <0.01~0.021, respectively. The amount of mandipropamid for unpeeled and peeled based on PHI were 0.250 and 0.014 mg/kg, respectively, and for diflubenzuron were 0.264 and 0.013 mg/kg, respectively. Thus, the result indicate that Koeran melon treated two pesticides should not be exported to Japan because both pesticides were detected over Japan MRL in peeled samples.

PFS-5

Analysis of sodium diacetate in foods by HPLC-DAD

Kun Ho An, Chan Lee, Ji Hyun Lee*

Department of Food Science and Technology, Chung-Ang University

Sodium diacetate has been used as an acidulant on processed meats and breads. Two methods (i.e. free acetic acid and sodium acetate analsys method) were proposed to analyze sodium acetate by Codex. Herein, we developed a method to analyze sodium diacetate in processed meat and bread by using high performance liquid chromatography coupled with diode array detector (HPLC-DAD). The method was optimized by comparing different mobile phase, HPLC column, and column temperature etc. The optimum mobile phase consisted of 0.2% m-phosphoric acid in isocratic mode. Sodium diacetate was separated on an Alltech Prevail Organic Acid column (250×4.6 mm, 5 μm) at 40°C. Linear range was between 20 to 2000 μg/mL with a good linearity (R2>1.000). The limit of detection (LOD) and limit of quantification (LOQ) were calculated through the ICH guideline and were 2.42 and 7.35 μg/mL, respectively.

AcknowledgementThis research was supported by a grant (18162MFDS009) from Korea Food & Drug Administration in 2018.

PFS-6

Investigation of the quality changes for dried pepper powder after UV-C irradiation

Ji-Yeon Bae1, Deuk-Yeong Lee1, Jin-Ho Rho2, Jin-Hyo Kim1*1Department of Agricultural Chemistry, IALS, Gyeongsang National University, Jinju,

2Chemical Safety Division, National Institute of Agricultural Science, RDA, Wanju

UV irradiation is one of the mostly considered the secure food sanitation process due to its easy application and no residue after treatment. Recently, UV-C irradiation was applied to remove microbial and/or chemical contaminants in food and successively the goal was achieved in some cases. However, UV-C can change food coloring matters such as anthocyanins and carotenoids. The microbial contamination of dried pepper powder usually issued and UV-C would be expected one of the option for microbial control. However, the UV-C treatment decreased the quality factor of dried pepper over 0.5 h. After four hours of UV-C treatment, water soluble pigment and capsanthin contents were decreased 14.5% and 33.7%, respectively.

PFS-7

Development of the Analysis and Content Evaluation Technology for Allergens in Rice

Sang Gu Lee*, Si Myung Lee, Seon Woo Oh, Soo Yun Park, Tae Hoon Ryu, Hyeon Jung Kang

Biosafety Division, National Institute of Agricultural Science

Allergic reaction means when organism comes into contact with an exotic substance that result in damage to cell in the body due to hypersensitive antigen-antibody reaction. Allergic diseases are classified as skin diseases and respiratory diseases depending on the target organ with symptoms. Especially when antigens are food, they are called food allergies. Currently, the food safety assessment of GM crops specifies the results of the substantial equivalence evaluation through the comparative analysis of nutrients and anti-nutrient components with existing crops, and research is being carried out to establish natural variations on the composition of domestic crop varieties, but the information on allergy substances contained in domestic crops is insufficient. So, This study was carried out based on information on the previously reported sequence of rice allergen to develop antibody-based allergen detection methods for quantitative analysis of them. It is expected that through this process, it will be possible to establish a database for natural variation and allowable range of allergy protein content for domestic rice varieties and to provide data on the safety assessment of GM crops.


252

PFS-12

Cassia tora seed extract improves type 2 diabetic condition through the regulation of pancreatic mitochondrial enzymes’

activities and secretion of adiponectin

Eun Ko1, Min Young Um2, Moonsung Choi3, Sooim Shin1,4*1Interdisciplinary Program of Bioenergy and Biomaterials Graduate School, College of

Engineering, Chonnam National University, 2Division of Functional Food Research, Korea Food Research Institute, 3Department of Optometry, College of Energy and

Biotechnology, Seoul National University of Science and Technology, 4Department of Biotechnology and Bioengineering, College of Engineering, Chonnam National University

Cassia tora is an annual herb, which has been reported to have pharmacological effects such as antioxidant, hypolipidemic and anti-diabetic effect. The whole plant, roots, and seeds have been widely used as traditional medicine. Accordingly, its efficacy on diabetic conditions such as hyperglycemia and hyperlipidemia had been studied. However, the anti-diabetic effect of cassia tora on mitochondrial function remains unclear. In this study, the effects of Cassia tora seed extracts (CSEE) on pancreatic mitochondrial function was examined in high-fat diet (HFD) fed mice. Also, several indicators of diabetes were measured to investigate its effect on insulin resistance. To these, effective compounds were extracted from cassia tora using 70% ethanol and they were treated on HFD mice for 12 weeks. As a result, HFD fed mice exhibited increased fasting glucose and insulin secretion, and decreased adiponectin representing its condition of insulin resistance. However, HFD with CSEE treated mice exhibited decreased blood glucose and insulin secretion, improved insulin sensitivity and increased adiponectin secretion. Moreover, CSEE enhances pancreatic mitochondrial complexes’ activity and increased expression level of them, compared with HFD fed mice. Taken together, these findings indicate that CSEE attenuates HFD-induced diabetic condition by hypolipidemic effect with increased adiponectin secretion and by enhanced pancreatic mitochondrial function resulting in appropriate insulin exocytosis and inhibition of apoptosis in pancreatic beta cell.

PFS-9

Application of Dispersive Liquid-liquid Microextraction for the Determination of Ochratoxin A in Grain Feeds

Mi Jeong Lee, Theresa Lee, Jeomsoon Kim, Ja Yeong Jang*

Microbial Safety Team, National Institute of Agricultural Sciences, Rural Development Administration

Ochratoxin A is one of the most important and deleterious mycotoxins. OTA is produced mainly by some strains of Aspergillus ochraceus and Penicillium verrucosum species, that occurs in a variety of grains, cereals, and other plant products and animal feeds. OTA causes nephrotoxicity and renal tumors in a variety of animal species. A method based on dispersive liquid-liquid microextraction (DLLME) combined with ultra-high performance liquid chromatography was developed for the quantitative extraction of OTA from grain feeds such as corn, barley, wheat, sorghum, and oat. Under the optimum conditions, the linear range were 1-200 ng/mL for OTA with the correlation coefficient (R2) of 0.9999. Limits of detection (LOD) were 10 ng/g, and the mean recoveries were in the range of 71.4-115.3% with RSD <19.5% (n =3). We compared DLLME method with conventional immunoaffinity column method; the recovery and RSD were comparable for the two methods, but the DLLME approach was more effective in terms of time and cost.

PFS-10

Occurrence of Fusarium Species and Mycotoxins in Unprocessed Cereals

Mi Jeong Lee, Jung-Hye Choi, Ju-Young Nah, Hyun-Suk Jin, Soobin Yim, Theresa Lee, Jeom-Soon Kim, Ja Yeong Jang*

Microbial Safety Team, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea

A total of 148 samples of oat (n=46), sorghum (n=43), adlay (n=41), and millet (n=18) were collected from fields in 2017 to identify mycotoxigenic fungal flora and to analyze the presence of Fusarium mycotoxin such as deoxynivalenol (DON), nivalenol (NIV), zeralenone (ZEA), fumonisin B1+B2 (FUM). When the fungal flora associated with each cereal samples were investigated, adlay was the most often contaminated with Fusarium spp. (55.5%), followed by the sorghum (36.9%), millet (20.6%), and oat (8.0%). Contaminants (mean and ranges) found in sorghum were DON (27.2 ng/g; ND~159.0 ng/g), NIV (45.5 ng/g; ND~206.0 ng/g), ZEA (28.2 ng/g; 11.1~55.9 ng/g), FUM (111.6 ng/g; ND~687.0 ng/g). Millet samples were contaminated NIV (50.3 ng/g; ND~149.0 ng/g) and ZEA (17.5 ng/g; ND~36.0 ng/g). Adlay samples were contaminated DON (39.6 ng/g; ND~656.0 ng/g), NIV (27.1 ng/g; ND~90.0 ng/g), and ZEA (8.4 ng/g; ND~32.1 ng/g). On the other hand, oat samples were largely free from mycotoxins. This is the first report of occurrence of the mycotoxins and mycotoxigenic Fusarium species on oat, sorghum and millet in Korea.

PFS-11

Application of Kinetic Models for Predicting the Degradation Rate of Triazole Pesticides in Perilla leaves

Hyo Jeong Kim1, Sang Hyeob Lee1*, Se Yeon Kwak1*, Aniruddha Sarker1*, Hye Rin Jeong1*, Ae Ji Nam1*, Tae Hwa Kim2*, Jang Eok Kim1*

1School of Applied Biosciences, Kyungpook National University, 2Analysis Technology and Tomorrow, Daegu 41566, Korea

Dissipation patterns of pesticides are especially significant for understanding the potential exposure in the environment. It is important to predict the biological half-lives of applied pesticides to ensure safety. In this study, three kinetic models (zero order (ZO), first order (FO) and second order (SO)) were employed to compare and characterize the best-fit kinetic model describing the residual pattern of triazole pesticides in perilla leaves as a minor crop. Triazole pesticides are familiar to minor crops so in this study we used pesticides such as tetraconazole and tebuconazole. Tetraconazole (12.5%, EW) and tebuconazole (25%, EC) were diluted with water and sprayed 3 times at an interval of 7 days before harvesting. The residual amounts of tetraconazole and tebuconazole were analyzed by GC-ECD and HPLC-UVD respectively. Comparing the correlation coefficients (R2) of the kinetic models, tetraconazole was 0.97, 0.98, and 0.97 for the ZO, FO, SO respectively and tebuconazole was 0.90, 0.97, and 0.98 for the ZO, FO, SO respectively. Therefore FO was best fit to Tetraconazole but SO was best fit the dissipation patterns of tebuconazole. The equations of biological half-life of tetraconazole were 16.1 days using FO (Ct=10.939e-0.043x) but 14.5 days, 18.4 days using ZO and SO. Furthermore, tebuconazole half-life was 5.4 days using SO (Ct=0.0258x+0.1428) but 8.3 days, 6.8 days using ZO and FO.

Poster Presentation


Poster Presentation

PFS-16

Effect of selected PGPRs on the initial growth of wheat cultivar

Sang Gyu Lee, Hyeri Lee, Dong Gun Lee, Jimin Lee, Na-Hyun Lee, Namhyun Chung*


Wheat is one of the most important grains, which of consumption increases globally. Many countries are making efforts to increase the extent of wheat harvest. This study was focused on the effect of plant growth-promoting rhizobacteria (PGPRs) on the initial growth of wheat cultivars. PGPRs as rhizosphere microorganism in soil have a beneficial effect on various plants. Most of PGPR have abilities of IAA production, phosphate solubilization, and/or siderophore production. We have isolated several PGPRs including Pseudomonas nitroreducens strain IHB (PnIHB) and Paenibacillus pabuli strain P7S (PP7S) from soil. The effect of PGPRs on early germination of wheat was studied with 3 wheat cultivars in Korea. When wheat seeds were treated with PGPRs, many aspects of germination and early growth were stimulated differently by PGPRs. It was also noticed that the degree of stimulation was dependent on the kind of wheat cultivars. All of these results are a preliminary study of how PGPRs are involved in the early growth of wheat due to some molecular biological effects. It is also suggested that treatment of PGPRs as a fertilizer component can increase wheat yield by promoting early growth of wheat. This work was supported by a grant (PJ01249602) from the Rural Development Administration, Republic of Korea.

PFS-13

Immature Citrus unshiu physiological activities during vinegar manufacturing: changes in polyphenolic contents

and in vitro antioxidant activity

Hyeok Jun Yang, Min Young Kim*

Toxicology Lab, Major in Biomaterials, Jeju National University

Vinegars based on fruit juices could conserve part of the health-associated compounds present in the fruits. However, in general very limited knowledge exists on the consequences of vinegar-making on different antioxidant compounds from fruit. In this study, two types of vinegars (Citrus vinegar or Citrus fruit pulp vinegar) derived from immature Citrus unshiu were evaluated for their bioactive compounds, antioxidant and other physiological capacities. The contents of total phenolic and flavonoid contents were measured using colorimetric method. In addition, free radicals scavenging, SOD-like activity, ferrous ion chelating and reducing power assays were used to determine the antioxidant capacity. Both vinegars were shown to be antioxidants with high contents of total phenolics and total flavonoids; Citrus fruit pulp vinegar exhibited higher antioxidant activity than that of Citrus vinegar, however, which was closely related to the contents of total phenolic and flavonoid. The beneficial health effects of immature Citrus vinegars may in part be related to the process-induced changes in their antioxidative phenolics during vinegar manufacturing.

PFS-14

Quality and bioactive properties of mulberry leaf according to blanching and hot-air dry conditions

Ji Hae Lee1, O-Chul Kwon1, Wan-Taek Ju1, Hyun-Bok Kim1, You-Young Jo1, Haeyong Kweon1, Young Bo Lee2*

1Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea, 2Technology Services Team, National

Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea

Mulberry (Morus alba. L) leaves contains various flavonoids including rutin, quercetin, iso-quercetin. Recent studies have reported antioxidant, hypoglycemic, and hypotensive activities of mulberry leaves, which are due to high level of bioactive compounds. In this study, we analyzed physicochemical properties of mulberry leaves according to blanching time and hot-air dry conditions. The mulberry leaves were harvested at the middle of the May, and then blanched for 1-10 minutes. The samples were dehydrated for 7 minutes, and hot-air dried at 40-70℃ for 5-2 hours. The quality of mulberry leaves were compared by hardness, color value, contents of polyphenols and antioxidant activities. The changes of physicochemical properties were observed according to the blanching time and hot-air dry conditions. These results will be utilized as basic data for the industrial use of mulberry leaves.

PFS-15

Profiling of Endogenous Metabolites in Multigrains Based on Metabolomics

Hyejin Yu1,2, Jeonghak Lee2, Songhee Ryu1, Jeong-Han Kim2*1Division of Chemical Safety, National Institute of Agricultural Sciences,

2Department of Agricultural Biotechnology, Seoul National University

According to a recent study, 78% of Koreans prefer multigrains. The main reason for preference of multigrains is that it is considered to be good for health. This study was carried out to determine the endogenous metabolites in multigrains that Koreans generally prefer. Five grains; white rice, brown rice, millet, foxtail millet and sorghum were obtained to conduct this study. GC-MS/MS and LC-MS/MS were utilized to analyze metabolites in the samples. The Automated Adjusted Retention Time (AART) function was used to adjust simultaneously the retention time of target compounds in GC-MS/MS. As statistical tool, Principal Component Analysis (PCA) and Partial least square Discriminant Analysis (PLS-DA) were used.


254

PFS-20

Real-time PCR based detection method for GM cotton and canola

Min Ki Shin*

Food Safety Risk Assessment Division, National Institute of Food and Drug Safety Evaluation

Genetically modified (GM) crops have been constantly developed and approved for commercialization. To make sure of GMO labeling regulations, GMO identification methods have been developed and verified.In this study, we validated detection methods for GM cotton, MON88702, and GM canola, LBFLFK, using real-time PCR (RT-PCR).The qualitative detection analysis of MON88702 and LBFLFK was carried by RT-PCR assessing specificity, sensitivity and repeatability.As a result, each detection method for MON88702 and LBFLFK was able to specifically detect only those event for other GM soy, corn, cotton and canola events. The detection limit of the method for MON88702 and LBFLFK was 0.01% and 0.005%, respectively.In conclusion, we validated detection method for GM cotton, MON88702, and GM canola, LBFLFK. The results showed that validated methods would be applicable for the detection to ensure the appropriateness of mandatory labeling for GMOs in Korea.

This research was supported by grant (19161MFDS052) from the Ministry of Food and Drug Safety Korea.

PFS-17

Relevancy of nitrate reductase and leaf rust resistance in Korean wheat

Hyeri Lee, Sang Gyu Lee, Dong Gun Lee, Jimin Lee, Minseo Kim, Namhyun Chung*


Wheat is one of the most important crop around the world. Leaf rust is one of the most common cause of wheat spoilage disease. Puccinia triticina is the most common pathogen among three rust diseases which can reduce the yield of wheat to 70%. Nitrate is one of the major sources of nitrogen for the growth of plants. It is taken up by plant roots and transported to the leaves where it is reduced to nitrite. The main objective of this research was to investigate relationship between nitrate reductase and leaf rust resistance in wheat plants. We isolated leaf rust pathogen, P. triticina, which was used to inoculate several Korean wheat and durum cultivars. Then, the infection levels were checked according to six infection types such as 0, 0’, 1, 2, 3 and 4. The six infection types were divided into two groups of more infected and more resistant groups, for each of which two cultivars were selected. We screened the relationship between nitrate reductase expression level and leaf rust level with the two groups. With further study, the obtained results would contribute to the development of enhanced resistance of cultivars to pests and disease. This work was supported by a grant (PJ01249602) from the Rural Development Administration, Republic of Korea.

PFS-18

The relationship between rice yield and the occurrence of extreme weather events in South Korea

Jina Hur, Kyo-Moon Shim*, Yongseok Kim, Kee-Kyung Kang


The climate change impact on rice productivity in South Korea was investigated with respect to a relationship between the rice yield and the occurrence of extreme weather events over 17-year span (1999-2015). First of all, we analyzed the changes of both rice yield and the frequency of extreme weather events (abnormally high/low temperatures, abnormally long duration of sunshine, and abnormally short duration of sunshine) over the time. Overall, the rice yield and the occurrence of abnormal weather events over South Korea have increased during this 17-year period. In the meteorological factors, the increase trend of abnormally high temperature is the highest, whereas one of abnormally low temperature is the lowest. On the basis of the correlation coefficient, the change of rice yield is negatively related with one of the occurrence of abnormally low temperature (-0.41 with 99% confidence level), while it is positively related with the abnormally long duration of sunshine (0.51 with 99% confidence level). It implies that the sufficient long duration of sunshine without extremely cold may be linked to the high productivity of rice in South Korea under the assumption of no technological adaptation to climate change.

This study was carried out with the support of Research Program for Agricultural Science & Technology Development (Project No. PJ01185802), National Institute of Agricultural Sciences, Rural Development Administration, Republic of Korea.

PFS-19

Detection of genetically modified canola DP-073496-4 and MON88302 using event-specific multiplex PCR

Ji-Eun Park, Jin-Young Choi*, Hyun-Joong Kim*, Hae-Yeong Kim *

Department of Food Science and Biotechnology, Kyung Hee University

Genetically modified (GM) canola has been grown for use as food-grade oil and animal feed. As of April 2019, the Ministry of Food and Drug Safety in Korea has authorized the ten GM canola events for food use in Korea. Previously, we performed the detection of eight genetically modified canola events (GT73, MS8, RF3, T45, MS1, RF1, RF2, and Topas 19/2) using two event-specific pentaplex PCR systems. In recent 5 years, two GM canola events (DP-073496 and MON88302) have been newly approved for food or feed in Korea. And the detection methods of these events are necessary for management of GM canola events. In this study, an additional multiplex PCR method was developed to detect these two events simultaneously. The limit of detection of this PCR assay was approximately 0.0125% using DNA mixed non-GM canola DNA with 2 GM canola events. Compared with the unintentional limit of 3% in Korea, this result demonstrated that this PCR method might be enough to detect specific GM canola event.

Poster Presentation


Poster Presentation

PFS-24

Effects of soybean varieties on quality characteristics of tofu

Eunyeong Sim*, Hye Young Park, Areum Cheon, Mi-Jung Kim, Jieun Kwak, Yong Hee Jeon, Choon Ki Lee

Department of Central Area Crop Science, National Institute of Crop Science, RDA

The objective of this study was to evaluate several quality properties of six soybeans (Saedanbaek, Daechan, Daepung2, Miso, Seonpung, Saegeum) and their effects on quality characteristics of tofu. The distributions of 100 seed weights and seed coat ratios were 22.86 g (Saedanbaek)~32.43 g (Saegeum) and 5.70% (Saegeum)~6.33% (Saedanbaek), respectively. Saedanbaek was the highest in protein and ash contents with the values of 45.88% and 5.38%. Proximate composition, composition of fatty acid, amino acid, dietary fiber were also investigated. Contents of phytic acid and total phosphorus ranged in 2.78 (Saegeum)~3.59 (Saedanbaek) g/100 g, 6066 (Daepung2)~7777 (Saedanbaek) mg/kg, respectively. The crude protein and lipid contents of soybeans seemed to affect the hardness of the tofu. There was a significant difference in the moisture and yield rate of the tofu among soybean varieties (p<0.05). Total solid contents of the tofu was about 20%. The weights of the tofu increased 1.8~2 times than the weights of soaked soybeans. There was no significant correlation between protein contents and yield rate of tofu when the contents were considered based on varieties. In sensory evaluations, significant differences were shown in taste, texture and overall acceptability in tofu made of different soybeans varieties.

PFS-21


Thu Thi Nguyen, Chi Rac Hong, Munkhbat Saranchimeg, Sang Min Kim*

Smart Farm Research Center, KIST Gangneung Institute of Natural Products

Asparagus (Asparagus officinalis L.) is rich source of phytochemicals, including antioxidants, phenolics, saponins and amino acids. Studies on asparagus revealed a wide range of therapeutic activities, such as anti-diabetic, anti-tumor, antifungal. The aim of this study was to evaluate the quality and functional ingredients of asparagus juice produced by different processing methods. Three processing methods were blending for physical method, hot-water extraction for thermal method, and pectolytic enzyme treatment from Aspergillus niger for enzymatic method, and two fresh asparagus (green and white asparagus) were used to make juice. After processing, the yield of juice solution was measured in a solid state, and antioxidant activity, ingredients such as phenolics, saponin protodioscin and amino acids were evaluated with the freeze-dried juice powder. In result with juice solutions, the enzymatic method showed the highest juice yield among three methods. The juice powder from the physical method contained the highest amount of total flavonoids in both asparagus while the juice powder from enzymatic method exhibited higher protodioscin amount in white asparagus. In addition, total phenolics, antioxidant activity and amino acids in both asparagus were changed significantly according to different processing methods. These results will give basic information in the selection of proper extraction method in the production of asparagus juice.

PFS-22


Jinyoung Choi, Jieun Park*, Hyunjoong Kim*, Haeyeong Kim*

Department of Food Science and Biotechnology, Graduate school of Biotechnology, Kyung Hee University

The value of cotton and the high adoption rate of the stacked cotton have increased the area of GM cotton cultivation in the world. In 2017, GM cotton account for 150,000 tons among 153,100 tons of whole imported cotton into Korea. Some cases have been reported that unintentional leaks of GM cotton into the natural environment caused by the increased GM cotton cultivation and distribution. According to the Biosafety Clearing-House of Korea, currently 13 single-events and 16 stacked-events of GM cotton were approved to import into Korea. Therefore, the effective detection and post-monitoring methods of GM cotton are needed to manage GM cotton efficiently and to prevent its contamination into natural environment in Korea. In this study, a multiplex PCR detection method of GM cotton was developed for faster and more efficient detection. A total of four sets of event-specific tetraplex PCR were performed for 12 single genetically modified cotton events (MON531, MON1445, MON15985, MON88701, MON88913, GHB119, GHB614, COT102, LLcotton25, 281/3006, DAS81910, T304-40ⅹGHB119) except for MON757 event that has ceased commercialization. The detection limit of each set showed a detection value ranging from 0.05% to 0.5% depending on each set, which is lower than the 3% threshold of the unintentional inclusion allowance of Korea.

PFS-23

A Review on Processing Factors of pesticide Residues During Potato Processing

Moo-Hyeog Im1*, Jeong Ah Kim1, Hye Su Lee1, Jeong-A Seo1, Seo Hong Kim2

1Food Engineering, Daegu University, 2Environmental and Biological Chemistry, ChungBuk National University

This study summarizes the processing factor (PF) of pesticide residue in potato processing from 2007 to 2016 JMPR reports. PF is being used in CODEX to establish the tolerance limit for pesticide residue of processed foods. Therefore, this study investigated the correlation between the pesticide physicochemical characteristics and pesticide reduction rate on PF of potato processed food evaluated by JMPR, and to make it possible to utilize the CODEX for setting the tolerance limit for pesticide residue for potato processing. Potatoes were compared to PF for 25 pesticides calculated through the processing in Washed, Peel, Baked, Boiled, Flakes, Chips, Fried, Dried, Starch. Among the six pesticides treated with washed, cycloxydim, flupyradifurone and sulfoxaflor were concentrated even after washing with oil soluble pesticide. When the peel was removed, most pesticides tended to decrease significantly. This showed that the pesticide remained on the surface and was greatly reduced due to the removal of the peel. In case of boiling, except for some pesticides, most pesticides showed a large decrease rate. The residues of the boiled potato which had been removed the peel decreased more than those of the boiled potato with peels. Flakes, chips and fried showed the tendency to decrease in most pesticides. But, 11 kinds of pesticides such as cyromazine, chlothianidin, cycloxydim and dinotefuran, which have water solubility characteristics, were concentrated.


256

PFS-28

Change of nutrient, ginsenoside, and volatile flavor constituents and antioxidant activity during food processing

of mountain-cultivated ginseng with the cocktail lactic acid bacteria

Su Cheol Kim1, Yong Min Kang2, Jin Hwan Lee3, Kye Man Cho1,2*1Department of Food Science, Gyeongnam National University of Science and Technology,

2Herbal Medicine Resources Research, Korea Institute of Oriental Medicine, 3Department of Life Resources Industry, Dong-A University

This study investigated that change of the nutrient (fatty acids, amino acids, and minerals), ginsenosides, and volatile flavor compounds and antioxidant activities during food processing of mountain-cultivated ginseng (MCG) with the cocktail lactic acid bacteria. The content of fatty acids was increased during processing, but the value of free amino acid was decreased and the minerals were little unchanged. The total phenolic and flavonoid contents and mallard reaction products increased markedly according to process stages. The levels of total ginsenosides increased from 31.25 mg/g (MCG) to 32.36 mg/g (RMCG) and then decreased (27.27 mg/g, at FRMCG) during processing. Particularly, the contents of F2 (0.31 →1.02→2.27 mg/g), Rg3 (0.36→0.77→2.9 mg/g) and compound K (0.5→1.68→4.13 mg/g) of ginsenosides and β-panasinsene (17.28%→22.69%→31.61%) of volatile flavors were increased during processing, respectively, corresponding to the effects of antioxidant increased. This result proposed that processed MCG may be contributed to enhance the ginseng value, which can then be used as nutritional and functional ginseng products, such as foods and cosmetics etc.

PFS-25

Multi-Analytical Method of Mycotoxin from Feed using LC-MS/MS

Hyeong Wook Jo1, Jeong Hoon Seon2, Kyu Won Hwang2, Joon Kwan Moon2*1Hansalim Agro-Food Analysis Center, Hankyong National University Industry Academic

Cooperation Foundation, 2Department of Plant Life and Environmental Sciences, Hankyong National University

Multi-analytical method using LC-MS/MS for mycotoxin from feed for monitoring was developed with QuEChERS preparation. After Sample weighing (5 g) in a 50 mL conical tube, 5% formic acid in distilled water (10 mL) and acetonitrile (10 mL) was added. After it was strongly shaken for 30 min, QuEChERS extraction salt (EN method) were added to the 50 mL conical tube. The mixture was strongly shaken for 1 min and was centrifuged at 4,000 rpm for 10 min. The acetonitrile layers (1 mL) were purified by dSPE (C18 25 mg, 50 mg, 100 mg, 200 mg) and centrifuged at 10,000 rpm for 5 min. The purified extract was filtered with a membrane filters (pore size: 0.2 um) before analysis. The result of this study confirm that LC-MS/MS methods are a good option for multi-mycotoxin. The 13 compounds could be accurately quantified in the 0.625~250 ug/kg concentration range with correlation coefficients>0.99. Matrix effect (ME, %) were -85.4~156.9% range and the percentage (of 13 mycotoxin) recovers were in the range 74.7~123.8% (AFB1,2, AFG1,2: 10 ug/kg, OTA, ZEN, FB1,2, α-ZAL, β-ZAL, T-2, HT-2: 50 ug/kg and DON 250 ug/kg).

PFS-26

Changes of phenolic composition and antioxidant activity of strawberries (cv. ‘Seolhyang’ and ‘Jukhyang’) during ripening

Dong Hee Ahn1, Jinwook Lee2*, Jihyun Lee1*1Department of Food Science and Technology, Chung-Ang University, Anseong, Republic of

Korea, 2Department of Plant Science and Technology, Chung-Ang University, Anseong, Republic of Korea

Strawberries (Fragaria ananassa) are rich sources of phenolics, especially anthocyanins. The phenolic content depends on maturity of strawberries; however, there is little information on the changes of phenolic composition of a newly developed strawberry cultivar, ‘Jukhyang’. Herein, phenolic profile of a traditional strawberry cultivar (cv. ‘Seolhyang’) and a newly developed strawberry cultivar (cv. ‘Jukhyang’) harvested at different ripening stages (i.e., green, pink, red, and over-ripened) were determined by high performance liquid chromatography coupled to diode-array-detector (HPLC-DAD). Additionally, total phenolic contents (TPCs), total flavonoid contents (TFCs), antioxidant activity (DPPH radicals scavenging activity), and total anthocyanin contents (TAC) were investigated in the strawberry samples. Pelargonidin-3-O-glucoside and cyanidin-3-O-glucoside were major anthocyanins found in strawberries regardless of cultivars and ripening stages. Additionally, catechin was found in the strawberry samples. Catechin contents were rapidly decreased during ripening; however, anthocyanin contents were continuously increased. From red to over-ripened stage, the amount of pelargonidin-3-O-glucoside in ‘Jukhyang’ and ‘Seolhyang’ strawberries increased 1.8-fold and 2.8-fold, respectively. ‘Jukhyang’ strawberries showed lower TPCs, TFCs, antioxidant activity than ‘Seolhyang’ strawberries at every ripening stage. However, ‘Jukhyang’ strawberries contained higher cyanidin-3-O-glucoside content than ‘Seolhyang’ strawberries, regardless of ripening stages. This indicates that anthocyanin composition can be different by cultivars and ripening stages of strawberries.

PFS-27

Change of the nutrient and phytochemical constituents and antioxidant and antiinflammatory effects from fresh to

processed mountain-cultivated ginseng sprouts

Su Cheol Kim1, Yong Min Kang2, Jin Hwan Lee3, Kye Man Cho1,2*1Department of Food Science, Gyeongnam National University of Science and Technology,

2Herbal Medicine Resources Research, Korea Institute of Oriental Medicine, 3Department of Life Resources Industry, Dong-A University

This study investigated that change of the nutrient (fatty acids, amino acids, and minerals) and phytochemical (ginsenosides, flavonols, and phenolic acids) constituents and biological activities from fresh to processed mountain-cultivated ginseng sprouts (MCGS). The content of free amino acids was decreased during processing, but the fatty acids and minerals were little unchanged. The total phenolic and flavonoid contents and mallard reaction products increased markedly according to process steps. the levels of total ginsenosides decreased from 45.06 mg/g (MCGS) to 31.74 mg/g (FAMCGS) during processing, but the amounts of flavonols and phenolic acid increased from 586.0 μg/g and 195.48 μg/g (MCGS) to 898.77 μg/g and 365.39 μg/g (FAMCGS), respectively. Particularly, the contents of Rg3 (0.98→2.9 mg/g, 2.9 times) and compound K (0.55→6.3 mg/g, 11.5 times) of ginsenosides, epigallocatechin (53.87→146.12 μg/g, 2.7 times) of flavonols, and ferulic acid (nd→62.43 μg/g) of phenolic acids increased during processing, respectively, corresponding to the effects of antioxidant and anti-inflammatory increased. This result proposed that processed MCGS may be contributed to enhance the ginseng value, which can then be used as nutritional and functional ginseng products, such as foods and cosmetics etc.

Poster Presentation


Poster Presentation

PFS-32

Development of Analytic Method for Maltitol in Mouthwash using High-Performance Liquid Chromatography coupled

with Evaporative Light Scattering Detector

Sangseop Kim1, Kyoung-Moon Han1, Jihyun Shin1, Soae Ji1, Rheeda Lee1, Soojeong Son2, Kyunghun Son1*

1Cosmetic Research Team, National Institute of Food and Drug Safety Evaluation, 2Pharmaceuticals and Medical Devices Research, National Institute of Food and Drug

Safety Evaluation

Maltitol has 75– 90% of the sweetness of table sugar and is used as a low-calorie sweetener because of its low absorption rate. It has been used as an additive in many pharmaceuticals, for example, mouthwash, cough drops and toothpaste. The Ministry of Food and Drug Safety (MFDS) has regulated the content of maltitol equivalent to 90.0– 110.0% of the indicated amount by Korean Quasi-drugs Codex (KQC). The aim of this study is to improve the test method of maltitol in KQC, which is a gas chromatography with packed columns. In this study, it is developed by using a high-performance liquid chromatography coupled with evaporative light scattering detector because of its simplicity and efficiency.The stationary phase of LC column was C8 (4.6×150 mm×5.0 µm) and mobile phase was acetonitrile, water and isopropanol (75:20:5, isocratic). The assay was validated with linearity, precision, and recovery rate. For the linearity, the calibration curve was drawn three times, and the R2 values were greater than 0.999. The limit of detection and limit of quantitation were 0.02 and 0.06% ppm, respectively. In addition, the precision was evaluated by relative standard deviation (RSD) of 6 repeated measurements at 0.5-4%, which was less than 3%. The recovery rate was in a range of 99.8-105.9% by measuring the content of maltitol in mouthwash products.

PFS-29

Comparison in phytochemical constituents (ginsenosides, phenolic acids, and flavonols) of antioxidant activities in above

and below grounds of ginseng sprouts for different roasting conditions

Ho Jeong Park1, Su Cheol Kim1, Du Young Cho1, Hee Sun Jung1, Jin Hwan Lee2, Kye Man Cho1,3*

1Department of Food Science, Gyeongnam National University of Science and Technology, 2Department of Life Resources Industry, Dong-A University, 3Herbal Medicine Resources

Research, Korea Institute of Oriental Medicine

In this study, the contents of phytochemicals including ginsenosides, phenolic acids, and flavonols and as well as antioxidant effects in in above and below grounds of ginseng sprouts (GSs) for different roasting conditions. The roasted GSs exhibited significantly higher antioxidant activity than unroasted GSs in the four antioxidant methods including DPPH, ABTS, and hydroxyl radical scavenging activities and Ferric reducing antioxidant power (FRAP). In particular, the above ground of GSs at 150℃ for 15 min showed the highest antioxidant activity in comparison with other samples. The levels of Rg3 and compound K of ginsenosides, chorogenic acid of phenolic acids, and quercetin of flavonols as well as the total phenolic and flavonoid contents and maillard reaction products increased during roasting process. Moreover, leading to a general increase in antioxidant activity after roasting. These results suggest that the roasting GSs could contribute to obtaining natural antioxidants in certain food and cosmetics applications.

PFS-30

Change of nutritional constituents and in vitro antioxidant activity during the food processing of isoflavone-enriched

soy leaves with the cocktail lactic acid bacteria

Du Young Cho1, Su Cheol Kim1, Ho Jeong Park1, Hee Sun Jung1, Sang Hee Nam1, Jin Hwan Lee2, Kye Man Cho1,3*



This study investigated that change of the nutrient (fatty acids and amino acids) and isoflavone contents and antioxidant activities during food processing of isoflavone-enriched soy leaves (IESL) with the cocktail lactic acid bacteria. The content of fatty acids was increased during processing, but the value of free amino acid was decreased. The total phenolic and flavonoid contents increased according to process stages. The levels of total isoflavone increased from 8923.09 μg/g (FrIESL) to 9977.34 μg/g (UFeIESL) and then decreased (6417.62 μg/g, at FeIESL) during processing. Particularly, the contents of daidzein (270.02→1705.71→3735.1 μg/g) and genistein (121.18→565.71→1386.01 μg/g) were increased during processing, respectively, corresponding to the effects of antioxidant increased. This result proposed that processed IESL may be contributed to enhance the ginseng value, which can then be used as nutritional and functional ginseng products, such as foods and cosmetics etc.

PFS-31

Change of γ-aminobutyric acid and isoflavone constituents and in vitro antioxidant activity during the lactic acid

fermentation of isoflavone-enriched soy leaves

Hee Sun Jung1, Su Cheol Kim1, Ho Jeong Park1, Du Young Cho1, Sang Hee Nam1, Jin Hwan Lee2, Kye Man Cho1,3*



This study investigated that change of the free amino acid and isoflavone contents and antioxidant activities during the fermentation of isoflavone-enriched soy leaves (IESL) with the cocktail lactic acid bacteria. The content of total free amino acids was decreased from 1152.25 mg/100 g (0 hr) to 863.72 mg/100 g (72 hr) during the fermentation of IESL, but γ-aminobutyric acid content was increased from 144.24 mg/100 g to 175.59 mg/100 g. The total phenolic and flavonoid contents increased during the fermentation of IESL. The levels of daidzin and genistin decreased from 4327.03 and 2459.23 μg/g (0 hr) to 3082.27 and 1211.84 μg/g (72 hr), while the contents of daidzein (749.27→2265.57 μg/g) and genistein (217.93→987.65 μg/g) were increased during the fermentation of IESL, respectively. The effects of antioxidant increased. This result proposed that processed IESL may be contributed to enhance the soy leaf value, which can then be used as nutritional and functional IESL products, such as foods and cosmetics etc.


258

PFS-36

Residual characteristics of pyrifluquinazon and its main metabolite (pyrifluquinazone-1H) in minor crops

Jiho Lee*, Kwanghun Lee, Suyoung Ju, Jinchan Kim, Kyujin Jung, Sangchul Kim

Bio Technology Division, Korea Conformity Laboratories

Pyrifluquinazon is known to insecticide that has been used to inhibit a feeding of insects in fruits and vegetables. In this study, we investigated the residual characteristics of pyrifluquinazon and its main metabolite (pyrifluquinazon-1H) in minor crops (perilla, shallot, leek, radish). The residual pesticide was analyzed by HPLC-MS/MS (Shimadzu LCMS-8050) equipped with OSAKA SODA C18 column.Method limit of quantitation (MLOQ) was 0.01 mg/kg. The linearities of calibration curves (r2) were ≥0.99 at the range of 0.005-0.2 mg/kg. For the recovery tests, 10 g of crops was fortified with standard solutions at 10 MLOQ and 50 MLOQ levels and extracted with 10 mL of acetonitrile by shaking (1300 rpm, 1 min). After extraction, 4 g of MgSO4 and 1 g of NaCl were added, shaken for 1 min and centrifuged at 4,000 rpm for 5 min. The supernatant was injected to HPLC-MS/MS after mixing with solvent (1:1, v/v). The results of recovery tests were in the range of 70-120% (RSD≤10%) at two fortified levels. For field trials, 0 day plot after spraying the pesticides of perilla (leaf), shallot, leek, radish (leaf and root) were 4.03 mg/kg, 0.78 mg/kg, 2.38 mg/kg, and 0.90 mg/kg, respectively.

PFS-33

GC/MS method development of ethyl carbamate analysis in various food matrices

Seung Min Kim, Sung Hyeon Jung, Myung-Sub Chung, Jihyun Lee*

Department of Food Science and Technology, Chung-Ang University, Korea

Ethyl carbamate (EC) is a probable carcinogen (IARC, Group 2A) and found in various fermented foods and alcoholic beverages. The aim of this study was to develop analytical methods for EC in high-fat solid (beef tenderloin), high-fat liquid (mayonnaise), low-fat solid (boiled egg white), and low-fat liquid (milk) food matrix. Differences from AOAC methods included a use of EC-d5 as internal standard. Removal of lipophilic compounds were performed by n-hexane extraction in order to improve extraction efficiency. The standard curve had a high linearity (R2=0.999) in all evaluated food matrices. The limit of detection (LOD) and limit of quantification (LOQ) were in the range of 0.10-0.43 ng/g and 0.30-1.29 ng/g, respectively. The spike-recovery test resulted in recovery rate of 95.8%-106.4%. Intra- and inter-assay precision ranged from 0.7% to 7.8% and 1.3% to 12.1%, respectively.

AcknowledgementThis research was supported by a grant (18162KFDA053) from Korea Food & Drug Administration in 2018.

PFS-34

Physicochemical analysis of Zanthoxylum schinifolium using HPLC

A Yeong Lee1, Hyo Seon Kim1, Goya Choi1*, Chang-Seob Seo2*1Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine,

2Herbal Medicine Research Division, Korea Institute of Oriental Medicine

The genus Zanthoxylum consistis of approximately 250 species, of which six are distributed in Korea. The Korean herbal pharmacopoeia lists the origin of this medicine as Zanthoxylum piperitum De Candolle, Zanthoxylum schinifolium Siebold et Zuccarini, and Zanthoxylum bungeanum Maximowicz. Zanthoxylum schinifolium is used in spice and treatment of ozena, rheumatoid, nasal sinusitis, sore throat etc, and its oil has been folk remedies to treat stomachache and asthma in Korea. This plant consist of coumarins, quinoline, alkaloids, and essential oil. The fruit of this plant were extracted in 70% ethanol (ZSE) and distilled water (ZSW), and then carried out physicochemical pattern analysis using HPLC. Schinifoline was detected in both the hexane fraction of ZSE and ZSW. The hexane fraction was mainly composed of oil, and the fatty acid of this part was analyzed by HPLC. Generally, GC has been used for the analysis of fatty acids following derivatization into their methyl esters. However, this method tend to be time-consuming, labor intensive, and difficult to quantify. In this study, fatty acids were analyzed using HPLC-CAD technique without derivatization. Herein we found that the hexane fraction of Z. schinifolium contains three unsaturated fatty acid (linolenic acid, linoleic acid, and oleic acid) and two saturated fatty acids (palmitic acid and stearic acid).

PFS-35

Chemical Properties Variation of Morus bombycis using Decompressed Heat Pump Dryer

Hee-Jung Park, Hyun-Soo Kim, Sang-Hyun Lee*

Department of Forest Environmental Science, Chonbuk National University

This study compared and analyzed chemical properties variation of Morus bombycis, using Decompressed Heat Pump Dryer (DHPD) and existing hot-air dryer. In order to examine chemical properties variation were measured the DPPH and ABTS radical scavenging activity, nitric oxide content in water extract of the DPHD, 70% ethanol extract of DPHD, hot-air dryer water extract and 70% ethanol extract of the hot-air dryer were measured. In results, DPPH and ABTS radical scavenging activity, DPHD was higher than hot-air dryer. Nitric oxide content, in water extract, DPHD was 46.7 higher than hot-air dryer and DPHD was 18.5 higher than a for 70% ethano l extract. DHPD can be used as an effective drying method for enhancing the quality of food since it shows a high content of chemical components, and the antioxidant activity as compared to the existing vacuum freeze drying method. In addition, DHPD is next-generation drying technology, which solve the issues such as the destruction of the nutrients and flavor of the ingredients appearing in hot-air dryer and the degradation of the unique characteristics of the products by the variation of the form.

※ This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry(IPET) through Technology Commercialization Support Program, funded by Ministry of Agriculture, Food and Rural Affairs(MAFRA)(816001-3)

Poster Presentation


Poster Presentation

PFS-40

Antifungal and antiaflatoxigenic activities of naphthoquinones towards Aspergillus flavus

Gayoung Kim, Hye Seung Choe, Sung-Eun Lee*


Aflatoxin is a mycotoxin produced by Aspergillus flavus and is known as a secondary metabolite that induces liver cancer in humans. They are consisted of aflatoxin B1, B2, G1, and G2. Foods, that are primarily infected by the fungi, are dominated by maize, peanuts, walnuts, and almonds. In addition, various physical disinfection methods as well as chemical methods, and biological methods have developed in markets to prevent the contamination of the fungi and aflatoxins. Recently, we have confirmed the inhibition on A. flavus growth and the production of aflatoxins by naturally occurring compounds derived from pepper plants such as piperlongumine, piperine, and piperonal, and food additives, including benzoic acid, and sorbic acid. Through the present study, antioflatoxic and antifungal activities of naphthoquinone-based substances were confirmed and their activities were verified in solid medium. Among the 10 natural substances used in the experiment, 1,4-naphthquinone was found to have strong antifungal effects according to the concentration gradient. Azoxystrobin (10 ppm) was used as the control group. In the future, the molecular mechanism of these antifungal and antiaflatoxigenic activities will be described.

PFS-37

Protective Role of Kimchi under Different Fermentation Stages against Amyloid Beta-Induced C6 Glial Cell Damage

Ji Myung Choi1, Eun Ok Choi1, Yung Hyun Choi2, Eun Ju Cho1*1Department of Food Science and Nutrition, Kimchi Research Institute, Pusan National

University, 2Department of Pathology and Research Institute of Korean Medicine, Anti-Aging Research Center, Dong-eui University

Kimchi is a fermented vegetable with health benefits. Baechu kimchi is the most representative one among many types of kimchi and Koreans eat various fermented states of it. We prepared three kinds of kimchi under different fermentation stage; Fresh kimchi (Fresh; pH 5.6 and acidity 0.3%), optimally ripened kimchi (OptR; pH 4.3 and acidity 0.64%), and over-ripened kimchi (OvR; pH 3.8 and acidity 1.24%). The present study examined protective role of ethanol extract from Baechu kimchi under different fermentation stages against amyloid beta 25-35 (Aβ 25-35)-induced damage in C6 glial cell. Accumulation of Aβ is known as an increasing risk of Alzheimer’s disease. Aβ 25-35-treated C6 cells decreased cell viability and elevated the reactive oxygen species (ROS) level. However, the treatment of kimchi extracts increased cell viability and decreased the ROS level. Also, Baechu kimchi extracts attenuated Aβ 25-35-induced neuronal damage by regulating mitogen-activated protein kinases family protein expression. Especially, OvR was significantly down regulated the extracellular signal-regulated kinase and p38 expressions. These results suggest that Baechu kimchi may be the beneficial food for preventing Aβ-induced neuronal damage.

PFS-38

Protective Effect of Samultang and Its Four Herbal Plants from Reactive Oxygen Species under in Vitro and Cellular System

Yu Ri Kwon1, Mi Na Nam1, Ah Young Lee2, Seung Mi Sin3, Young Min Goo3, Eun Ju Cho1*

1Department of Food Science and Nutrition, Pusan National University, Busan 46241, Republic of Korea, 2Department of Food Science, Gyeongnam National University of

Science and Technology, Jinju 52725, Republic of Korea, 3Gyeongnam Oriental Anti-aging Institute, Gyeongnam 52215, Republic of Korea

Overproduction of free radical and oxidative stress have been reported to a major pathological hallmark of neurodegenerative diseases. The aim of the present study is to investigate the antioxidant and protective effects of Samultang, and its four herbal plants, Paeonia lactiflora (PL), Ligusticum striatum (LS), Rehmannia glutinosa (RG), and Angelica gigas (AG), under in vitro and cellular system. The fractions of Samultang strongly increased 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical (·OH), and nitric oxide radical (NO) scavenging activity in a concentration-dependent manner. Furthermore, we investigated the protective effects of Samultang on cellular damage against oxidative stress induced by hydrogen peroxide (H2O2) in SH-SY5Y cells. The treatment of Samultang alleviated the oxidative stress by H2O2 through increasing cell viability and decreasing intracellular reactive oxygen species levels. Based on these results, we further studied the radical scavenging effects of PL, LS, RG, and AG. Among them, PL showed the highest of DPPH, ·OH, and NO radical scavenging activity. These data implies that the PL plays in Samultang a crucial role, involved with antioxidative and neuroprotective effects. The present research suggests that Samultang and its four herbal plant, especially PL, play the protective role against oxidative stress.

PFS-39

Simultaneous analysis of fungicide tecloftalam and its metabolites tecloftalam imide in rice using LC-MS/MS

Junghak Lee, Jong Hwa Lee, Yongho Shin, Ji-Ho Lee, Min Woo Jung, Jeong-Han Kim*


The fungicide Tecloftalam is widely used for the control of bacterial leaf blight in paddy rice. The aim of this study was to development of simultaneous analytical method for tecloftalam and tecloftalam imide in rice using LC-MS/MS for residue monitoring. For the determination in LC-MS/MS (Shimadzu LC-MS 8060), negative electrospray ionization mode and selected reaction monitoring was employed using Kinetex C18 anlytical column (2.1×100 mm, 2.6 µm) for peak separation. The mobile phase was acetonitrile and water containing 2 mM ammonium acetate. The method limit of quantitation (MLOQ) was 0.05 µg/mL. The linearity of matrix-matched calibration curve (r2) was ≥0.98 at the calibration range of 0.01~0.5 mg/kg. For the preparation of sample, 20 mL of acetonitrile was added to 5 g of sample powder before shaking (20 min). For partitioning, 4 g MgSO4 and 1 g NaCl were added before shaking (1 min) and centrifugation (3,000 rpm, 10 min). The final matrix-matched supernatant (5 µL) was injected to LC-MS/MS. A recovery test was performed at two fortification levels (10 MLOQ, 50 MLOQ) to validate the established method. The recovery rate was in the range of 77.5~114.9% with 4.5~7.9% of relative standard deviation (RSD). The analysis method developed in this study proved to be suitable for simultaneous quantitative determination of tecloftalam and its tecloftalam imide in rice.


260

PFS-41

Dissipation pattern of fungicide validamycin A in Sorghum bicolor after treatment of 20% water soluble powder

during cultivation

Mai Rehan, Junghak Lee, Xiu Yuan, Seohyun Kim, Eunyoung Park, Jeong-Han Kim*


Validamycin A has been used to control sheath-blight disease. We established field experiment using a minor crop, Sorghum Bicolor. For field trials (n=3) the crop was treated with validamycin A (20% water soluble powder 1,000 fold dilution) at 50/40/30, 40/30/21, 30/21/14 and 21/14/7 DBH (days before harvest). Validamycin A was analyzed on LC-MS/MS (Shimadzu LCMS-8060) with HILIC column (2.1×100 mm, 1.6 µm). Mobile phase were water and acetonitrile with 0.1% formic acid. The method limit of quantification was 0.01 mg/kg and the linearity was ≥0.99 (0.002~0.5 mg/kg). For recovery test 5 g of grain or leaves was spiked with standard solution at level of 0.01 and 0.05 mg/kg, extracted with 25 mL of 50% methanol and centrifuged at 3,000 rpm for 5 minutes. Then (1 mL) was transferred to tube containing 10 mg GCB to be vortexed for 5 minutes at 14,000 rpm for purification. The matrix matched extract (200 μL) was injected. The recoveries range for grain sample was from 75~101% and for leaves sample was from 82~91% with RSD ≤10%. The initial residue of validamycin A in grain sample at 21/14/7 DBH was 0.06 mg/kg and decreased to 0.04 mg/kg at 50/40/30 DBH. In leaves sample 0.04 mg/kg of initial residue decreased to 0.03 mg/kg.

PFS-42

Hair growth promoting effect of 4HGF encapsulated with PGA nanoparticles (PGA-4HGF) by β-catenin activation

and its related cell cycle molecules

Hye-Ji Lee, Ha-Kyong Kwon, Hye-Jin Park*

Department of Food Science and Biotechnology, College of BioNano Technology, Gachon University, Gyeonggi-do 13120, Republic of Korea

Poly-γ-glutamic acid (γ-PGA)-based nanoparticles draw remarkable attention as drug delivery agents due to their controlled release characteristics, low toxicity, and biocompatibility. 4HGF is an herbal mixture of Phellinus linteus grown on germinated brown rice, Cordyceps militaris grown on germinated soybeans, Polygonum multiflorum, Ficus carica, and Cocos nucifera oil. Here, we encapsulated 4HGF in PGA-nanoparticles to facilitate its penetration into hair follicles (HFs). In this study, we report the hair promoting activity of 4HGF encapsulated with PGA nanoparticles (PGA-4HGF) and their mechanism. The average size of spherical nanoparticles was ~400 nm in diameter. Continuous release of PGA-4HGF was observed in a simulated physiological condition. As expected, PGA-4HGF treatment increased hair length, induced earlier anagen initiation and elongated the duration of anagen phase in C57BL/6N mice, compared with 4HGF treatment. PGA-4HGF significantly increased dermal papilla cells proliferation and induced cell cycle progression. PGA-4HGF also significantly increased total amount of β-catenin protein expression, a stimulator of anagen phase, through induction of cyclinD1 and CDK4 protein levels, compared to 4HGF treatment. Our findings underscore the potential of PGA nanocapsules to efficiently deliver 4HGF into HFs, hence promoting hair-growth. Therefore, PGA-4HGF nanoparticles may be promising therapeutic agents for hair growth disorders.

Poster Presentation


Poster Presentation

PAM-4

In Silico Optimization of 16S rRNA Gene Primer Pairs for Exploring Bacterial Diversity in Microbial Community

Amplicon Analysis

Huy Quang Pham, Kerfahi Dorsaf, Yeon-Gyun Jung, Seung-Dae Choi, Minji Kim, Hyun-Woo Son, Jae-Ho Shin*


Targeted amplicon sequencing of 16S rRNA genes has become the dominating tool used for studying microbial community. However, determining which hypervariable regions and primer sets should be used for analyzing microbial communities requires considerable inspection. Therefore, there is the demand for computational methods to design optimal bacterial 16S primers able to achieve accurate taxonomy information provided by the new sequencing technologies. In this study, we propose a computational method for optimizing the choice of important primer sets based on different hypervariable regions included V12, V13, V34 and V45 and against various databases. The results show that our strategy is able to find a better primer pair on V45 region than the ones available in the literature. Despite validation from amplicon community sequencing, we still believe that through in silico analysis, our improved primers should be widely utilized for further amplicon studies.


PAM-1

Optimization of multienzyme production from Aspergillus sp. for use in Retting Process of pineapple leaf fiber

Juthamas Suwanprateep, Paweena Thongkred*, Thidarat Nimchua*

BIOTEC, NSTDA

At present, harvesting the fruits leaves an enormous amount of leaves, which are considered as waste materials. However, the pineapple leaf fibers (PALF) composed of 70-82% cellulose, have the proven potentials to produce yarns and fabrics. In this study, enzymatic retting of PALF was thus developed in order to extract cellulose fibers compared to water and chemical retting process. After optimization of enzyme production factors, optimized conditions obtained from solid state fermentation of A. aculeatus BCC17849 yield the maximum xylanase and pectinase activities of 525.43 ±35.48 and 263.61 ±2.96 u/g, respectively with 3 days of cultivation time at 30°C. To test enzyme efficacy, retting solutions were adjusted to contain 100 u/g fresh fiber of pectinase used to ret the fibers at 40°C and pH 4.5 for 1 day with a liquor ratio of 1:10 on an incubator shaker at 100 rpm. Controls included retting of the PALF with 0.5%(w/v) of urea treatment for 7 days and water-retted treatment for 14 days were done in parallel. As compared with the controls (water and chemical retted samples), treatment with enzyme solutions resulted in significant increase in reducing sugar liberated from the fibers, illustrating of the enzyme ability to ret the PALF. Scanning electron micrographs also revealed that the separation and fibrillation of multienzyme-retted PALF appeared to be better when compared to the control treatments.

PAM-2

Conversion of l-arabinose to l-ribulose using genetically engineered Candida tropicalis

In-Seok Yeo, Jung Hoe Kim*

Department of Biological Sciences, Korea Advanced Institute of Science and Technology

For the biological production of l-ribulose, conversion by enzymes or resting cells has been investigated. However, concentrated substrates, an additional purification step to remove borate, and the requirement for a cell cultivation and harvest steps before utilization of resting cells make the production process expensive and complex. Microbial fermentation may help overcome these limitations. In this study, we constructed a genetically engineered Candida tropicalis strain to produce l-ribulose by fermentation with a glucose/l-arabinose mixture. For the uptake of l-arabinose as a substrate and conversion of l-arabinose to l-ribulose, two heterologous genes, l-arabinose transporter and l-arabinose isomerase genes, were constitutively expressed in C. tropicalis under the GAPDH promoter. The Arabidopsis thaliana-originated l-arabinose transporter gene (STP2)-expressing strain exhibited a high l-arabinose uptake rate of 0.65 g/L/h and the expression of l-arabinose isomerase from Lactobacillus sakei 23K showed 30% of conversion (9 g/L) from 30 g/L of l-arabinose. Furthermore, glucose catabolite repression was overcome by the constitutive expression of l-arabinose isomerase under the GAPDH promoter. This genetically engineered strain can be used for l-ribulose production by fermentation using mixed sugars of glucose and l-arabinose.

PAM-3

The prevalence of tetracycline-resistant bacteria and detection of ribosomal protective proteins (RPP) encoding genes of tetO,

tetQ, tetS and tetW in soil samples collected from selected agricultural fields and animal houses in Gimje, Iksan and

Jangsu areas

Kathyleen Nogrado1*, Heesu Jeon2, Jae-Young Cho1,2, Ji-Hoon Lee1,2

1Department of Agricultural Chemistry, Chonbuk National University, 2Department of Bioenvironmental Chemistry, Chonbuk National University

This study aims to determine the presence of commonly detected tetracycline genes in agricultural soil samples. The study utilized both the viable cell count and molecular methods to estimate the prevalence as well as tetracycline-resistant bacteria per gram of soil. The highest and least total culturable tetracycline-resistant bacteria was from Gimje: GAF-1 at 3.46×103 CFU/g and GAH-1 at 1.40×101 CFU/g. On the other hand, the highest prevalence of the total culturable bacteria was from Iksan IAF-3. Result showed that 47% of the total culturable bacteria 6.0×103 CFU/g was tetracycline-resistant. Additional information on the tetracycline resistance among Gram-negative bacteria in soil samples was also acquired, its prevalence ranged from 0.10% to 1.76%. Meanwhile, results of the conventional PCR showed that among the four genes, tetQ was the most prevalent in all collected samples. Further analysis using real-time PCR to determine the absolute quantification of tetracycline resistance expressed in average gene copy number per gram of soil (gene copy/g) was performed, and qPCR results showed that the average tetO gene was highest in Iksan (IAH-3) at 9.46×105 gene copy/g while tetQ was highest in Gimje agricultural field 1 (GAF-1) at 1.24×105 gene copy/g. Further study to examine the distribution of antibiotics-resistant genes and potential effects of the animal houses on the agricultural areas remains to be performed.


262

PAM-8

Characterization of a fungal disease caused by Phialemonium curvatum in Lentinula edodes

Min Keun Kim*, Soon Ae Sim, Si Lim Choi, Kwang Pyo Hong

Environmental Agriculture Research Division, Gyeongnam Agricultural Research and Extension Services

Lentinula edodes has traditionally been cultivated on hardwood logs, mainly oak (Quercus spp.) in order to obtain fruiting bodies for human consumption. Recently sawdust cultivation is becoming more common and getting increase. Fungal contamination is a important factor affecting the stage of inoculation, spawn running, mycelia browing, and growth of fruit body. In 2018, unusual symptoms on L. edodes were observed in mushroom farm in Haman-county of Gyeongnam Province. The mycelia of the pathogen covered and swallowed the myceila of L. edodes, and result in a crack on the surface of media. Finally, the media can not develope the fruit-body and show the abnormal symptom with black color in the infected region. The fungal mycelia were 2.2-3.1μm wide, septate and branched. The optimal temperature and pH for mycelial growth was 30℃ and 6.0~8.0. The phylogenetic tree obtained from the ITS sequence analysis showed that the isolated fungal pathogen corresponded to Phialemonium curvatum (100.0%). This report will help the farmers to understand the characterization of a fungal disease on L. edodes caused by P. curvatum.

PAM-5

Isolation and Characterization of IAA-producing Bacillus Consortia on Initial Growth Promotion of Arabidopsis thaliana

and Lettuce (Lactuca sativa L.)

Jimin Lee, Hyeri Lee, Dong Gun Lee, Na-Hyun Lee, Sang Gyu Lee, Namhyun Chung*


A promising method to improve plant growth is application of bio-fertilizer containing plant growth-promoting rhizobacteria (PGPR) strains instead of synthetic chemicals. Rhizospheric microbes have immense potentiality to synthesize and release various compounds, which include materials for adjustment of soil physicochemical property as well as for promotion of plant growth. Previous studies suggested that, among various traits of PGPR, production of indole-3-acetic acid (IAA) may be the first trait to be considered for application of PGPR. With various soil samples, six PGPR strains with outstanding IAA production activity were isolated and examined for other plant growth promoting activities such as siderophore production, phosphate solubilization, HCN production, and protease activity. All those isolates had at least one more positive feature, suggesting that the six strains are high potential for application to plant growth. The 16S rDNA sequence of those PGPRs was analyzed for bacterial identification. The extent of plant promoting activity of isolated PGPR strains were evaluated via seed germination test and pot experiments in growth chamber using Lactuca sativa L. and Arabidopsis thaliana. This work was supported by a grant (116118-3) from IPET (Korea Institute of Planning and Evaluation for Technology in Food Agriculture and Forestry).

PAM-6

Methods for Development of Bio-Fertilizers Containing Plant Growth-Promoting Rhizobacteria (PGPR) and Plant Growth

Stimulants in Horticultural Crops

Na-Hyun Lee, Jimin Lee, Hyeri Lee, Dong Gun Lee, Sang Gyu Lee, Namhyun Chung*


Plant growth-promoting rhizobacteria (PGPR) have been recognized as a promising alternative to chemical fertilizer. One of the major challenges in the development of sustainable bio-fertilizer is to assure consistent cell viability of the inoculum. Non-spore forming bacteria PnIHB was particularly concerned, since their cell viability decreases sharply due to stress factors during application at the site. As a largely unexplored way of protection, zeolite was suggested as a shelter or carrier that has microporous structure for protection from environmental stresses. It was found that starch and gelatin have the highest performance for granulation of necessary components (e.g., humic acid, amino acid, glycerol). These two additives were further tested for conservation of cell viability and nutrient supplement to PnIHB. This study aimed to prove its preservation of cell viability of PnIHB inside pellets. This shelf life experiment was performed at two different temperatures of room temperature and 35℃. Samples from the pellet including zeolite as microbial carrier were taken every week to test the cell viability by obtaining CFU. Suitable combinations (e.g., zeolite+starch, zeolite+gelatin) was found to help maintenance of cell viability. We plan to examine the survival rate of PnIHB inside pellets up to next 6 months. This work was supported by a grant (116118-3) from IPET (Korea Institute of Planning and Evaluation for Technology in Food Agriculture and Forestry).

PAM-7

Addition of Salt in the Incubation Media Induces Active Growth of the Symbiotic Bacteria in Entomopathogenic Nematodes

Awdhesh Mishra1, Yeon Su Yu2, Sang Heyong Park2, Kwang-Hyun Baek1*1Department of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 38541,

Republic of Korea, 2Research Division, Daedong Tech Co., Gyeongsan, Gyeongbuk 38401, Republic of Korea

Entomopathogenic nematodes in the genera of Steinernema and Heterorhabditis are environmentally beneficial insect controlling agents without toxicity to human being. Especially, the symbiotic bacteria Xenorhabdus spp. and Photorhabdus spp. in the nematodes are the major factors inducing the death of insects and larvae. The most important factors for the success of the solid-culture system is inoculation of the symbiotic bacteria Xenorhabdus nematophils and Photorhabdus luminescens in the nematodes, however, there have been problems of successive incubation of the bacteria. Recent finding of adding salt in the media increased the growth rate significantly, therefore, the massive gene expression was analyzed to identify the key factors for the growth. In this presentation, the current status of of entomopathogenic nematodes research along with the genes responsible for the active growth of the symbiotic bacteria are discussed.

Poster Presentation


Poster Presentation

PAM-9

Biocontrol Activity of Photorhabdus Species against the Streoptomycin-Resistant Plant Pathogenic Bacteria

Muhammad Fazle Rabbee, Md. Sarafat Ali, Kwang-Hyun Baek*

Department of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 38541, Republic of Korea

Photorhabdus species maintain a complex life cycle in a symbiotic interaction with entomopathogenic nematodes from the family Heterorhabditis and also involve a pathogenic interaction with insect larvae. The nematode-bacteria pair infects and kills insects. These bacteria are rich in biosynthetic gene clusters that produce bioactive specialized metabolites that prevent saprophytes and other microbial competitors from invading the insect cadaver. Photorhabdus produce various antimicrobial compounds such as anthraquinone, stilbene, mevalagmapeptides, photopyrones, glidobactin, and carbapenem, etc. Here we have shown that addition of L-proline and monosodium glutamate in the culture broth enhanced the metabolic activity of Photorhabdus species against wild type and streptomycin resistant plant pathogens. HPLC-MS analysis of the metabolic extracts also indicated the upregulation of certain compounds and inducing the metabolic shift in the bacteria.

PAM-10

An integrated understanding of Xanthomonas pathogenesis controlled by twocomponent regulatory systems using

transcriptome analysis

Mi-Ae Lee, Youngchul Yoo, Sujin Kim, Abdulwahab Antar, Sang-Won Lee*

Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University

The Gram-negative bacterium Xanthomonas oryzae pv. oryzae (Xoo) is the causal agent of bacterial leaf blight disease of rice. Xoo possesses intracellular signaling pathways, known as two-component regulatory systems (TCSs), which control a broad spectrum of biological processes in the pathogenic bacteria. In this study, we constructed a total of 62 inactivated mutants of the response regulatory (RR) genes to identify novel TCSs, which are involved in pathogenicity of Xoo. Upon the virulence test with the mutant strains, six mutant strains showed dramatically reduced virulence and have been selected for further analysis. To investigate the role of six response regulators (RRs) in Xoo pathogenicity, we examined transcriptome analysis of the mutant strains in rich medium PSA and host mimic media XOM2 using RNA-seq analysis. A total of 1,653 differentially expressed genes (DEGs) were identified (|log2 fold change|≥1) in the six mutant strains and these DEGs were enriched including biosynthesis of the secondary metabolite, bacterial secretion system, and glycolysis/gluconeogenesis. In addition, gene co-expression network analysis showed that transcription factors (TFs) and other RRs are likely associated with the identified RRs for Xoo virulence. To understand the integrated regulatory mechanisms of the disease mediated by TCSs, further analysis is in progress.

PAM-11

Plasma activated water selectively enhances infected macrophage apoptosis

Chaebok Lee1, Hwa-Jung Kim2*1Department of Medical Science, Chungnam National University, 2Department of Medical

Science and Department of Microbiology, Chungnam National University

Plasma effects can be quite selective, meaning tunable between damage to pathogenic organisms without damage to the host, or activation of different pathways in different organisms. In spite of its fatal effect in human mortality, pathogenic Mycobacterium tuberculosis (Mtb) have not yet been implied in a plasma— induced apoptosis effects in cells. In this study, we investigated the apoptosis of macrophages infected with pathogenic mycobacteria by plasma-activated water treatment. Plasma activated water (PAM) selectively enhanced higher infected macrophage apoptosis. Confocal microscopy showed a change in the cell membrane from intact to bleached upon exposure to PAM. qRT-PCR results show that Mtb cells secreted a large amount of thiol-related oxidoreductase and antioxidant enzymes. The macrophage apoptosis of PAM treatment is dependent on high hydrogen peroxide concentration, ROS production, and redox imbalance. This finding explains that the apoptosis efficacy of PAM is related to the toxicity of OH and H2O2 radicals in cells.


264

Poster Presentation


Poster Presentation


266

Poster Presentation


Poster Presentation

Bio-exhibition


270


부스번호 기관명 부스번호 기관명

1 YMC 10 마이크로자임

2 (주)켐온 11 한국생명공학연구원 생물자원센터(KCTC)

3 시마즈 사이언티픽 코리아 12 뷰키코리아

4 워터스코리아 13 한국생명공학연구원 해외생물소재센터

5 엔사이언스 14 (주)엠제이사이언스

6 영인과학(주) 15 (주)포바이오코리아

7 한국원자력연구원 첨단방사선연구소 16 영인프런티어

8 한국한의학연구원 17 안전성평가연구소 호흡기질환 제품 유효성평가연구단

9 한국한의약진흥원 한의약소재은행사업 18 (주)성문시스텍

Bio-exhibition Information

Convention Hall


Bio-exhibition

Bio-exhibition

YMC 부스번호 : 1

취급품목 Preparative HPLC, Column, Packing material, Micro reactor

CEO 김현택 대표전화 031-603-1321 전자메일 [email protected]

담당자 강훈철 대표팩스 031-716-1630 홈페이지 www.ymckorea.com

주　소 경기도 성남시 분당구 판교로 255번길 9-22, 310호

(주)켐온 부스번호 : 2

취급품목 연구대행 자문 및 비임상시험 보고서

CEO 송시환 대표전화 031-888-6634 전자메일 [email protected]

담당자 이건우 대표팩스 031-888-6640 홈페이지 www.chemon.co.kr

주　소 경기도 수원시 영통구 광교로 147 경기바이오센터 15층

시마즈 사이언티픽 코리아 부스번호: 3

취급품목LC, GC, LC/MS, LC/MS/MS, GC/MS, GC/MS/MS, UV/VIS Spectrophotometer 등 분석기기 및 표면/시험기기

CEO 타지마 와타루 대표전화 02-540-5541 전자메일 [email protected]

담당자 조윤성 대표팩스 02-540-2163 홈페이지 www.shimadzu.co.kr

주　소 06108 서울시 강남구 논현로 609 9층 (논현동, 팍스타워)

워터스코리아 부스번호: 4

취급품목 액체크로마토그래피, 질량분석기, 컬럼 및 소모품 등

CEO 이남구 대표전화 02-6300-9200 전자메일 [email protected]

담당자 이현숙 대표팩스 02-6300-9200 홈페이지 www.waters.com/kr

주　소 서울시 영등포구 여의공원로 101, 905-7호 워터스코리아

엔사이언스 부스번호: 5

취급품목 진공펌프, 오일펌프, Aspiration 시스템

CEO 나지수 대표전화 02-6013-4268 전자메일 [email protected]

담당자 나지수 대표팩스 02-6442-4268 홈페이지 www.nscience.co.kr

주　소 경기도 안양시 동안구 시민대로 230 (관양동, 아크로타워지니센터D-1133)

272


영인과학(주) 부스번호: 6

취급품목 분석기기 판매 및 렌탈 서비스

CEO 백종웅 대표전화 02-519-7300 전자메일 [email protected]

담당자 한주현 대표팩스 02-519-7400 홈페이지 http://www.youngin.com

주　소 (우: 06030) 서울시 강남구 압구정로 28길 22 구정빌딩 6층

한국원자력연구원 첨단방사선연구소 부스번호: 7

취급품목 연구기술용역

CEO 위명환 대표전화 063-570-3001 전자메일 [email protected]

담당자 권경진 대표팩스 063-570-3049 홈페이지 www.kaeri.re.kr/arti

주　소 전라북도 정읍시 금구길 29 첨단방사선연구소

한국한의학연구원 부스번호: 8

취급품목 CAP 식치연구 성과 전시

CEO 고병섭 대표전화 042-868-9509 전자메일 [email protected]

담당자 육진아 대표팩스 042-868-9293 홈페이지 https://www.kiom.re.kr

주　소 대전광역시 유성구 전민동 461-24

한국한의약진흥원 한의약소재은행사업 부스번호: 9

취급품목 생물전환 추출물, 분획물, 단일화합물 등

CEO 이응세 대표전화 053-810-0396 전자메일 [email protected]

담당자 소재현 대표팩스 053-801-0279 홈페이지 http://kmmb.nikom.or.kr

주　소 경상북도 경산시 화랑로 94 (갑제동)

마이크로자임 부스번호: 10

취급품목 유인평판트랩(종이, PP), 돌발해충 포충 트랩, 미생물용 배지

CEO 심영근 대표전화 061-363-0607 전자메일 [email protected]

담당자 오재준 대표팩스 061-363-0608 홈페이지 http://www.microzyme.co.kr

주　소 전남 담양군 담양읍 태봉로 97, 본관 2층


Bio-exhibition

Bio-exhibition

한국생명공학연구원 생물자원센터(KCTC) 부스번호: 11

취급품목 미생물, 식물세포주, 동물세포주, 미세조류 등의 자원 기탁 및 분양

CEO 김성건 대표전화 063-570-5603 전자메일 [email protected]

담당자 김소연 대표팩스 063-570-5609 홈페이지 http://kctc.kribb.re.kr

주　소 전라북도 정읍시 입신길 181 한국생명공학연구원

뷰키코리아 부스번호: 12

취급품목 실험실용 회전증발농축기, 융점측정기, 근적외선 분광기, 크로마토그래피 시스템

CEO로저바우만, 피우스슐러

대표전화 02-6817-7500 전자메일 [email protected]

담당자 이경희 대표팩스 02-6718-7599 홈페이지 www.buchi.com

주　소 서울특별시 금천구 디지털로 130, 301호 (가산동, 남성프라자)

한국생명공학연구원 해외생물소재센터 부스번호: 13

취급품목 해외 유용식물 추출물

CEO 김장성 대표전화 042-860-4306 전자메일 [email protected]

담당자 박미진 대표팩스 042-860-4309 홈페이지 http://www.ibmrc.re.kr

주　소 대전시 유성구 과학로 125 한국생명공학연구원 해외생물소재센터

(주)엠제이사이언스 부스번호: 14

취급품목 HPLC Systme & column, Western 장비, MPLC 시스템 & 소모품

CEO 안병철 대표전화 042-932-8930 전자메일 [email protected]

담당자 문석태 대표팩스 042-932-8935 홈페이지 http://www.mjscience.com

주　소 대전시 유성구 테크노2로 199, 미건테크노월드 1차 402호

(주)포바이오코리아 부스번호: 15

취급품목 기초실험소형장비, 시약 및 기자재, 실험소모품 외

CEO 서형종 대표전화 02-2061-8033 전자메일 [email protected]

담당자 이성민 대표팩스 02-2061-8034 홈페이지 http://www.forbio.co.kr

주　소 서울시 금천구 가산디지털2로 14, 대륭테크노타운 12차 1107호

274


영인프런티어 부스번호: 16

취급품목과학기기(GC, LC 소모품, 용매, QuEChERS(캐쳐스), pH meter, tip, pipette, plasticware등), 항체(의뢰 제작서비스 포함), 대사체 분석 서비스

CEO 이덕희 대표전화 02-2140-3300 전자메일 [email protected]

담당자 안대근 대표팩스 02-2140-3310 홈페이지https://www.younginfrontier.com/laboratory

주　소 서울특별시 금천구 벚꽃로 244, 1101호

안전성평가연구소 호흡기질환 제품 유효성평가연구단 부스번호: 17

취급품목 유효성평가서비스

CEO 이규홍 대표전화 063-570-8773 전자메일 [email protected]

담당자 김경애 대표팩스 063-570-8897 홈페이지 http://www.ncer-kit.com

주　소 전북 정읍시 백학 1길 30, 전북흡입안전성연구본부

(주)성문시스텍 부스번호: 18

취급품목 UHPLC, HPLC, GC 컬럼 및 시료전처리(SPE, QuEChERS) 제품 및 소모품

CEO 김문해 대표전화 02-2201-5881 전자메일 [email protected]

담당자 노혜정 대표팩스 02-2201-5796 홈페이지 http://smst.co.kr

주　소 서울특별시 동대문구 청계천로 471, 대성스카이렉스 2차 오피스 4층

Author Index


276


AAbul Kasem, Mohammad Mydul Islam PES-33

Ahmad, Sajjad PBM-58

Ahn, Dong Hee PFS-26

Ahn, Eun-Kyung PNB-5, PNB-6

Ahn, Ginnae GS4-9, PNB-112, PNB-113,

PNB-114, PNB-127

Ahn, Ji Hye PBM-96

Ahn, Jihye PBM-98

Ahn, Joong Hoon PBM-32, PBM-34

Ahn, Joong-Hoon PBM-29, PBM-30, PBM-31,

PBM-33, PBM-37

Ahn, Philip PBM-16

Ahn, Seunghyun PNB-55

Ahn, Sun-Young PNB-130

Akauliya, Madhav PBM-14

Alam, Seemi Tasnim PNB-88

Ali, Md. Sarafat PAM-9

Altenbach, Susan B. IS1-1, PBM-45, PBM-46

An, Eesul PNB-94, PNB-96

An, Kun Ho PFS-5

An, Nan-Hee PES-17

An, Ren-Bo PNB-118

An, Seungjin PBM-99

Antar, Abdulwahab PAM-10

Arefin, M. Taufique IS2-2

Asamenew, Gelila GS4-4, GS4-8, S4-3

BBae, Chang Hwan PBM-22

Bae, Chang-Hwan PNB-53

Bae, Eun-Kyung PBM-89

Bae, Eunji PES-4

Bae, Ji-Yeon PFS-6

Bae, Jong Hyang PBM-85, PBM-93, S6-2

Bae, Joon-Yong PBM-13

Bae, Mun Gyeong PNB-41

Baek, Ji Yun PNB-104

Baek, Jun-Seok PNB-49

Baek, Ka-Yeon PNB-32

Baek, Kwang-Hyun PAM-7, PAM-9, PES-52

Baek, Nam-In GS3-5, PNB-19, PNB-20, PNB-21, PNB-22

Baek, Seung Cheol PBM-91

Baek, Seung Joon GS1-1, GS1-4

Baek, Seung-A PNB-83

Baek, Sujin PBM-76, PES-7, PES-33, PES-38

Bahn, Yong-Sun S5-1

Bai, Hyoung Woo PNB-51, PNB-52

Bai, Hyoung-Woo S3-1

Baiseitova, Aizhamal PNB-16

Bak, Dong Ho PNB-51, PNB-52

Bak, Dongho PBM-49

Ban, Jae-Suk PNB-77

Ban, Yeong Jun PNB-17

Bang, Kyungil PBM-59

Bang, Woo Young PBM-22

Bark, Sang-Won PES-45, PNB-72

Beak, Seung Joon PNB-23

Bhoo, Seong Hee PBM-4, PBM-56, S1-1

Bodelier, Paul L.E. S2-2

Boo, Kyung-Hwan PBM-73

Bunch, Heeyoun PBM-61

Byun, Hong-Joo PNB-29

Byun, Na Young PNB-61

CCaburatan, Lorrenne PBM-27

Cao, Viet Dang PBM-73

Cha, Hye Jin PNB-130

Cha, Seung Ju PES-46, PES-47, PES-48

Chae, Hee-Eun PNB-62

Chae, Songhwa PBM-90, PBM-102

Chang, Ancheol PES-3, PES-4

Cheon, Areum PFS-24

Cheon, Ye Eun GS1-8

Cheong, Jae Chul GS2-2, PES-60

Cho, Du Young PFS-29, PFS-30, PFS-31

Cho, Eun Ju PFS-37, PFS-38, PNB-7, PNB-109,

PNB-110, PNB-111, PNB-115

Cho, Ga Eul PNB-71

Cho, Hae Jin PNB-42, PNB-43, PNB-44

Cho, Hye Kyung PFS-1

Cho, Hyeon Ji PES-54

Cho, Hyun Nho PFS-4



Author Index

Cho, Il Kyu PES-40

Cho, In-Gyu GS2-5

Cho, Jae Young PES-12, PES-13, PES-14, PES-15,

PES-16, PES-21, PES-22, PES-23, PES-25,

PES-26, PES-28, PES-30, PES-31, PES-32

Cho, Jae Young Cho S2-1

Cho, Jae-Young PAM-3

Cho, Jung-Lai PES-17

Cho, Kye Man PFS-27, PFS-28, PFS-29,

PFS-30, PFS-31

Cho, Kyoung Oh PNB-119, PNB-126

Cho, Kyoungwon PBM-46, PBM-47, PBM-48

Cho, Man-Ho PBM-56

Cho, Moonjae GS3-6, PBM-11, PBM-35

Cho, Myoung-Lae PBM-91

Cho, Somi Kim PBM-35

Cho, Soo Yeon PNB-90

Cho, Su-Yeon PNB-50

Cho, Yoonsung PBM-100

Cho, Young Rak PNB-129

Choe, Hye Seung PFS-40, PNB-117

Choe, Jeong Sook PNB-90

Choe, Jeong-Sook PNB-50

Choe, Sanggil S7-2

Choi, Bo-Ram PBM-26, PNB-36, PNB-40, PNB-77

Choi, Bu-Ung PES-58

Choi, Buung PES-61

Choi, Byung-Yoon PBM-88

Choi, Chang Min GS3-6

Choi, Changmin PBM-11

Choi, Chun Whan PNB-5, PNB-6

Choi, Doil PBM-51

Choi, Doo Jin GS1-3, PNB-36

Choi, Eun Jung PES-27

Choi, Eun Ok PFS-37

Choi, Eun-Jeong PBM-42

Choi, Geun-Hyoung PES-45, PNB-72

Choi, Goya PFS-34, PNB-106

Choi, Gyu Sik PBM-32

Choi, Hee-Don S4-1

Choi, Hong-Kyu PBM-52, PBM-54, PBM-57

Choi, Hoon GS2-4

Choi, In-Geol PBM-9

Choi, Ji Myung PFS-37

Choi, Jihye PBM-40

Choi, Jin-Young GS4-2, PFS-19

Choi, Jinyoung PFS-22

Choi, Jiwon PBM-89

Choi, Jung-Hye PFS-10

Choi, Moonsung PBM-41, PBM-59, PBM-95, PBM-96,

PBM-97, PBM-98, PBM-99, PBM-101,

PES-74, PFS-12

Choi, Sang Ho GS3-2

Choi, Seon-A PNB-9, PNB-10, PNB-15

Choi, Seung-Dae PAM-4

Choi, Seung-Hyeon IS5-2, S5-3

Choi, Si Lim PAM-8, PES-54

Choi, Sung-Deuk GS2-5, PES-65

Choi, Won-Gyu IS1-3

Choi, Woo IS5-3

Choi, Woo-Jung PES-66, PES-67, PES-70, PES-72, PES-73

Choi, Yongmun PBM-18

Choi, Yongsoo PNB-83

Choi, Yoram PBM-52, PBM-54, PBM-57

Choi, Yu Min PNB-63

Choi, Yun-Hyeok PNB-6

Choi, Yung Hyun PFS-37

Chon, So Hyun PNB-47

Chong, Youhoon PBM-30

Choo, Byung Kil PNB-100, PNB-102, PNB-103

Choo, Hye Jeong PBM-32, PBM-34

Chu, Dong PBM-17

Chun, Jae-In PBM-43, PNB-68, S6-3

Chung, Byung Yeoup PBM-49, PNB-51, S3-1

Chung, In Sik PNB-41

Chung, Moon-Soo PBM-36

Chung, Myung-Sub PFS-33

Chung, Namhyun PAM-5, PAM-6, PBM-9,

PFS-16, PFS-17, PNB-66

Clark, John M. PL-1

DDao, Quang Minh S3-2

Dey, Debasish Kumar GS3-9

Dias, Mawalle Kankanamge GS4-9, PNB-112,

Hasitha Madhawa PNB-113, PNB-114

Dinesh Madusanka, PNB-112

Disanayaka Mudiyanselage

Ding, Tian-Bo PBM-21

278


Dorsaf, Kerfahi PAM-4

Dung, Ngo Thi Phuong IS4-3

EEo, Hyun Ji PNB-64

Eom, Ju Hyun PES-55

Eom, Mi Kyung IS5-2

Eom, Sanung PBM-24, PES-9, PNB-2, S1-2

FFürstner, Alois S3-2

GGang, Hyo-Seung PBM-92

Gautam, Avishekh PBM-14

Ghatge, Sunil PBM-67

Gil, Yeji PES-53

Go, Boram PBM-69

Go, Woo Ri PES-50

Gong, Ryong PNB-77

Goo, Young Min PBM-44, PES-35, PFS-38

Guang, Jin Hong PNB-118

Guo, Chenliang PBM-17

Gupta, Ravi GS1-8, PBM-84

Gwag, Jung Eun GS3-5

HHa, Bo-Keun GS1-7, PNB-107

Ha, Hyong-Ho PBM-92

Ha, Ji Hye PNB-119, PNB-120, PNB-121, PNB-126

Ha, Lee Su PNB-99, PNB-101

Ha, Taehwan PES-19, PES-20

Hahn, Bum-Soo PBM-1, PBM-2, PBM-3

Ham, Hyeon Mi PNB-84, PNB-85

Ham, Hyeon-Mi PNB-79, PNB-80

Ham, Young-Min PBM-68

Han, Cuong Viet GS2-3, PES-34

Han, Da Ye PBM-31

Han, Deug-Woo PES-19, PES-20

Han, Eui Jeong GS4-9, PNB-112, PNB-113,

PNB-114, PNB-127

Han, Jae Seung PNB-61

Han, Jaehong PNB-116

Han, Kook-Il IS5-2

Han, Kyoung-Moon PFS-32

Han, Song-I PBM-68, PBM-69, PBM-70,

PBM-71, PBM-72

Han, Soo-Jung PBM-28

Han, Soon-Ki IS1-2

Han, Yangsoo PES-56

He, Mei Tong PNB-110

He, Meitong PNB-7

Heo, Jae Young PES-54

Heo, Jae-Kyung PBM-78

Heo, Jung PBM-85, PBM-93, S6-2

Heo, Soo-Jin PNB-113

Hong, Chi Rac GS4-3, PFS-21

Hong, Eunkyung PNB-49

Hong, Gyunghoon IS3-2

Hong, In- Hong PBM-17

Hong, Kwang Pyo PAM-8

Hong, Seong Su PNB-5, PNB-6

Hong, Su-Myeong S8-1

Hong, Suk-Whan AL-2, PBM-8, PBM-10

Hong, Sung Tae PNB-89

Hong, Won-Ki PBM-43, PNB-68, S6-3

Hong, Young-Jin PES-43

Hong, Yu Kyung GS1-4

Huh, Eun Young S5-1

Huh, Tae Lin PBM-53

Hur, Hor-Gil PBM-67, PES-63

Hur, Ji Na PES-1

Hur, Jina PFS-18

Hwang, Bang Yeon GS3-2

Hwang, Byung Soon PNB-50

Hwang, Dae-Yeon PBM-94

Hwang, Duk-Ju PBM-102

Hwang, Ee Taek PNB-74, PNB-75, PNB-76

Hwang, Hyun Young PES-24, PES-68

Hwang, Hyun-Young PES-10



Author Index

Hwang, In Guk PNB-50

Hwang, Joo Tae PNB-19, PNB-22, PNB-69

Hwang, Kyu Won PFS-25

Hwang, Kyu-Won PES-49

Hwang, Kyung A PNB-90

Hwang, Se-Wook PES-51

Hwang-Bo, Jeon PNB-40, PNB-41

Hwang-bo, Jeon PNB-78

Hyeon, Hyejin GS1-8, GS4-5

Hyun, Byung Keun PES-50

Hyun, Chang Woo PNB-6

IIbal, Jerald Conrad PBM-58

Im, Moo-Hyeog PFS-23

Im, Seon-Yeong PES-43

Im, Seong Mi PNB-54

Im, Wan Teak PNB-92, PNB-93, PNB-95

JJang, Da Hyun PNB-18

Jang, Dae Sik PNB-56

Jang, Daeheon PBM-38, PBM-82

Jang, Geupil PBM-12

Jang, Hae Won S4-1

Jang, Hwan Hee PNB-90

Jang, Hwan-Hee PNB-50

Jang, Hyun-Jae PNB-82

Jang, Ja Yeong PFS-9, PFS-10

Jang, Jeong Su PES-2

Jang, Jeong Woo PBM-84

Jang, Jin Hoon GS1-6, PBM-55

Jang, Ki Chang PNB-84, PNB-85

Jang, Ki-Chang PNB-79, PNB-80

Jang, Su-Nyeong PBM-66, PBM-78

Jang, You-Ran GS4-1

Jang, Yu Na PES-19, PES-20

Jang, Yu Sin PES-6

Jang, Yu-Sin PBM-25

Jannat, Iskakova IS4-2

Jee, Youngheun GS4-9

Jeon, Da Bin PNB-119, PNB-120, PNB-121, PNB-126

Jeon, Doin GS3-3, GS3-8

Jeon, Heesu PAM-3

Jeon, Hwang-Ju PES-42, PNB-117

Jeon, Jin-Woo GS2-5

Jeon, Jong-Rok PNB-73

Jeon, Junhyun S5-2

Jeon, Sang Ho PES-50

Jeon, Woo-Jin PNB-62

Jeon, Yong Hee PFS-24

Jeon, Yong Sam S6-1

Jeon, You-Jin GS4-9

Jeong, Ah-Hyeon PNB-10, PNB-11, PNB-12,

PNB-13, PNB-14, PNB-15

Jeong, Chan Young PBM-10

Jeong, Da Hye PBM-69, PBM-70, PBM-71, PBM-72

Jeong, Dahye PBM-68

Jeong, Dong Kyu PBM-44, PES-35, PES-36

Jeong, Gyeong Han PNB-4

Jeong, Hae Jin PNB-73

Jeong, Hye Rin PFS-11

Jeong, Hye-Rin GS2-1, GS2-6

Jeong, In Hong PBM-21

Jeong, Jae Cheol GS1-7, PBM-86, PNB-107, PNB-108

Jeong, Min-Gyun PBM-52, PBM-54, PBM-57

Jeong, Seong Hun PNB-119, PNB-120, PNB-121,

PNB-126

Jeong, Won Min PBM-44

Jeong, Won Sik PNB-5

Jeong, Yong Seob S4-2

Jeong, Young-Jae PES-66, PES-67

Jeong, Yu Jeong GS1-7, PBM-86, PNB-107, PNB-108

Jeong,, Won Min PES-35

Jeoung, Seounghun PBM-59

Jho, Eun Hea PES-8

Ji, Soae PFS-32

Jin, Hyun-Suk PFS-10

Jin, Se-Eun PNB-49

Jin, Zhehao GS4-6

Jo, Gwanggon PES-19, PES-20

Jo, Hyeong Wook PFS-25

Jo, Ick-Hyun PBM-26

Jo, Jehyeon PNB-4

Jo, Yang Hee GS3-2, PNB-81

Jo, You-Young PFS-14

280


Jo, Young Jae PBM-58

Joen, Hee Won PNB-54

Joh, Won-Bae PES-43

Joo, Sun-Woo PNB-21

Joo, Yong Hun PNB-66

Ju, Boeun GS4-6

Ju, Suyoung PFS-36

Ju, Wan Taek PBM-5, PBM-6, PNB-8

Ju, Wan-Taek PFS-14

Jumapili Ramadhani, Fatuma PNB-52

Jun, Hui Soo PES-12, PES-13, PES-14, PES-15,

PES-16, PES-21, PES-22, PES-23, PES-25,

PES-26, PES-28, PES-30, PES-31, PES-32

Jun, Kyong Mi PBM-90, PBM-102

Jung, Choonkyun PBM-38, PBM-82

Jung, Dong Ho PNB-69

Jung, Gi Soo PNB-91

Jung, Goo-Bok PES-61

Jung, Hee Sun PFS-29, PFS-30, PFS-31

Jung, Hyeonjung PBM-79

Jung, Hyun-Ah GS4-4

Jung, Kwang-Woo S5-1

Jung, Kwangwoo PBM-49

Jung, Kyujin PFS-36

Jung, Kyung Su PES-49

Jung, Kyungsook PNB-127

Jung, Min Woo PFS-39

Jung, Min Woong PES-19, PES-20

Jung, Si Young PNB-63

Jung, Sung Hyeon PFS-33

Jung, Won-Kyo GS3-7

Jung, Woo-Jin PBM-16

Jung, Yeon Wo PNB-5

Jung, Yeon-Gyun PAM-4

Jung, Yong-Hwan PBM-68

Jung, Young Sung PNB-21

KKang, A-Hyeon PBM-80

Kang, Chang Hu PES-63

Kang, Chon-Sik PBM-8

Kang, Chon sik PBM-9

Kang, Dae-Won PES-69

Kang, Gi-Ung PBM-58

Kang, Hyeon Jung PFS-7

Kang, Hyeong Joong PBM-100

Kang, Igojo GS1-9

Kang, In Sun PES-75

Kang, In-Sun PES-71

Kang, Jae-Shin PNB-5, PNB-6

Kang, Jin-Ho PBM-43, PNB-68, S6-3

Kang, Jung Mi PFS-4

Kang, Kee Kyung PES-1

Kang, Kee-Kyung PFS-18

Kang, Ki Sung PNB-104

Kang, Kyungrai PNB-55

Kang, Kyungsu GS3-4, PNB-88

Kang, Mi Sook PBM-68, PBM-69, PBM-70,

PBM-71, PBM-72

Kang, Min Sung PBM-85

Kang, Min-Ah PNB-62

Kang, Min-Seung PNB-9, PNB-14

Kang, Myung-Gyun PBM-91

Kang, Myung-Ji PBM-20, PBM-50

Kang, Se Chan PNB-20

Kang, Se Won IS5-2

Kang, Seong Hee PBM-49, PNB-51, PNB-52

Kang, Seung Hye PNB-49

Kang, Shin-Young PNB-106

Kang, So-Mi PBM-70, PBM-71

Kang, Sun Chul AL-1, GS1-5, GS3-9

Kang, Tong Ho PNB-20, PNB-22

Kang, Yeongyeong PNB-64, PNB-65

Kang, Yong Min PFS-27, PFS-28

Kang, Youngmin PNB-33, PNB-34, PNB-35

Kasiviswanathan, Dharanibalan GS3-6, PBM-11, PBM-35

Kaur, Shubhpreet PBM-36

Kesawat, Mahipal Singh PBM-3

Kil, Young Sook PBM-44, PES-35

Kim, Bo-Eun PBM-87

Kim, Bo-Mi PNB-37

Kim, Bong-Gyu PBM-30, PBM-31, PBM-81

Kim, Cha Young GS1-7, PBM-86, PNB-107, PNB-108

Kim, Chae Yeon PNB-49

Kim, Chaelin PNB-26

Kim, Chang Jo PES-7

Kim, Chang Sook PBM-73

Kim, Da Som PNB-64

Kim, Da Young PNB-67



Author Index

Kim, Da-Hye PBM-68, PBM-70, PBM-71, PBM-72

Kim, Da-Young PES-69

Kim, Dada-Hye PBM-69

Kim, Dae Wook PNB-42, PNB-43, PNB-44

Kim, Dae-Choul PNB-92, PNB-93, PNB-95

Kim, Dae-Ok PNB-21

Kim, Danbi PBM-76, PES-7, PES-33, PES-38, PFS-8

Kim, Don-Kyu PBM-87, PBM-88

Kim, Dong Bum PBM-14

Kim, Dong Hee PNB-45, PNB-105

Kim, Dong Young PNB-122, PNB-123, PNB-124

Kim, Doo-Young PNB-81

Kim, Eun Ji PBM-31

Kim, Eun Ju PNB-61

Kim, Eun-A PNB-113

Kim, Euyeon PES-53

Kim, Ga Ryun PNB-92, PNB-93, PNB-97

Kim, Gayoung PFS-40

Kim, Geum Soog PNB-36

Kim, Geum-Soog GS1-3, PBM-26, PNB-77,

PNB-78, PNB-118

Kim, Gun Yeob PES-27

Kim, Hae-Yeong GS4-2, PFS-19

Kim, Haeyeong PFS-22

Kim, Han PBM-37

Kim, Han-Woo GS3-7

Kim, Han-Yong PES-66, PES-67, PES-72, PES-73

Kim, Hee Sook PES-2

Kim, Hee-Kwon PNB-24, PNB-25, PNB-60

Kim, Heejin S6-3

Kim, Heon-Woong GS4-4, GS4-8, S4-3

Kim, Ho Myeong GS2-3, PES-34

Kim, Hoon PBM-91, PBM-92, PES-18

Kim, Hui-Ju PNB-9, PNB-10, PNB-11, PNB-12,


Kim, Hwa-Jung PAM-11

Kim, Hyang Eun PNB-61

Kim, Hye Jin PNB-118

Kim, Hyeon Jin PFS-4

Kim, Hyeon Jung PNB-90

Kim, Hyeon-Young GS3-3, GS3-8

Kim, Hyeongseok PBM-83

Kim, Hyo Jeong PFS-11

Kim, Hyo Jung PNB-45, PNB-105

Kim, Hyo Seon PFS-34

Kim, Hyo-Jeong GS2-1, GS2-6

Kim, Hyoung-Geun GS3-5, PNB-19, PNB-20,

PNB-21, PNB-22

Kim, Hyun Bok PBM-5, PBM-6, PNB-8

Kim, Hyun Hee PNB-61

Kim, Hyun Jeong PNB-50

Kim, Hyun Jin PES-49

Kim, Hyun Young PNB-84, PNB-85, PNB-104,

PNB-109, PNB-115

Kim, Hyun-Bok PFS-14

Kim, Hyun-Hee PNB-62

Kim, Hyun-Ji PBM-78

Kim, Hyun-Joong GS4-2, PFS-19

Kim, Hyun-Soo GS4-9, PFS-35

Kim, Hyun-Young PNB-79, PNB-80

Kim, Hyunjoong PFS-22

Kim, In-Hwa PNB-62

Kim, In-Hyeon GS3-3, GS3-8

Kim, In-Young PBM-87

Kim, Inhwan GS4-7

Kim, Jae Hoon PBM-68, PBM-69, PBM-70, PBM-72

Kim, Jae Kwang GS1-8, GS4-5, PNB-83

Kim, Jae-Hoon PBM-71

Kim, Jae-Yean PBM-77

Kim, Jang Eok PFS-11

Kim, Jang-Eok GS2-1, GS2-6, S8-3

Kim, Jeom-Soon PFS-10

Kim, Jeomsoon PFS-9

Kim, Jeong Ah PFS-23

Kim, Jeong Ho PNB-18

Kim, Jeong Hun PNB-4

Kim, Jeong Yoon PNB-16, PNB-18

Kim, Jeong-Han GS2-2, GS4-6, PFS-15,

PFS-39, PFS-41, S7-4

Kim, Jeong-Monn PNB-14

Kim, Jeong-Moon PNB-12

Kim, Ji Hye PNB-48, PNB-61

Kim, Ji Hyun PNB-7, PNB-109, PNB-110

Kim, Ji Myung PES-40

Kim, Ji Young PNB-91

Kim, Ji-Hye PES-43

Kim, Ji-Sun IS5-2

Kim, Ji-Yeun Kate GS3-7

Kim, Ji-Youn PES-2

Kim, Ji-Young PNB-56

Kim, Jihae PBM-77

Kim, Jin Kyu PNB-5, PNB-6, PNB-129

282


Kim, Jin Soo PNB-45, PNB-105

Kim, Jin Sook S8-2

Kim, Jin Young PES-60, S7-1

Kim, Jin-Cheol GS2-3, IS2-3, PES-34, PNB-53, PNB-54

Kim, Jin-Hong PBM-36

Kim, Jin-Hyo PFS-6

Kim, Jin-Hyun PBM-52, PBM-54, PBM-57

Kim, Jinchan PFS-36

Kim, Jinsoo PBM-13, PBM-14

Kim, Jiwon PBM-73

Kim, Jiyoung PES-3

Kim, Jongmoon PNB-53

Kim, Joo Hong PNB-126

Kim, Joung Sug PBM-90, PBM-102

Kim, Ju Hong PNB-119, PNB-120, PNB-121

Kim, Ju Hyeon PBM-21

Kim, Jua PNB-49

Kim, Jung Hoe PAM-2

Kim, Jung-Bong GS4-4, GS4-8, S4-3

Kim, Jungeun PNB-45, PNB-105

Kim, Junheon PNB-46

Kim, Junse PNB-38, PNB-39

Kim, Ki Hwan PBM-80

Kim, Ki-Young PNB-57

Kim, Kicheon GS3-3, GS3-8

Kim, Kin Kyu PBM-49

Kim, Kwan-Woo PNB-118

Kim, Kwang-Sun GS1-2, GS3-1, PBM-75

Kim, Kyeong-A PFS-2

Kim, Kyeongnam PES-41, PES-42, PES-64

Kim, Kyungmun PBM-21

Kim, Li Yeon PNB-91

Kim, Mannyun PNB-46

Kim, Mi-Jung PFS-24

Kim, Mihyang PBM-30

Kim, Min A PNB-47

Kim, Min Jeong PNB-109, PNB-115

Kim, Min Jo PNB-7

Kim, Min Ju GS4-9, PBM-21, PNB-112,

PNB-113, PNB-114

Kim, Min Keun PAM-8

Kim, Min Suk PES-75

Kim, Min Young PBM-39, PFS-13, PNB-48

Kim, Min-Chul PBM-58

Kim, Min-Kyu S5-1

Kim, Min-Suk PES-71

Kim, Minji PAM-4

Kim, Minseo PBM-9, PBM-64, PFS-17

Kim, Mun-Ock PBM-20, PBM-50

Kim, Myeong-Hui PES-58

Kim, Myung Hee PES-62

Kim, Myung Sook PES-10, PES-24

Kim, Nam Ho PFS-1

Kim, Pyoung Il IS5-4

Kim, Ranhee PNB-56

Kim, Sang Gon PBM-44, PES-35

Kim, Sang Min GS4-3, PFS-21, PNB-70

Kim, Sang Yong PNB-95

Kim, Sang Yoon S2-2

Kim, Sangchul PFS-36

Kim, Sangseop PFS-32

Kim, Se In PES-62

Kim, Seahyun PBM-62

Kim, Semi PBM-95, PES-74

Kim, Seo Hong PFS-23

Kim, Seo-Young GS4-9

Kim, Seohyun GS4-6, PFS-41

Kim, Seong Heon PES-24

Kim, Seong-Heon PES-5, PES-10

Kim, Seong-Min PBM-43, PNB-68

Kim, Seung Min PFS-33

Kim, Sewon PBM-46

Kim, Sohyun PBM-95, PBM-96

Kim, Songyi PBM-29, PBM-31, PBM-37

Kim, Soon Ok PNB-53, PNB-54

Kim, Soyoung GS1-7, PNB-107, PNB-108

Kim, Su Cheol PFS-27, PFS-28, PFS-29,

PFS-30, PFS-31

Kim, Su-Hwa IS1-3

Kim, Su-Yeon PBM-3

Kim, Su-Young PNB-49

Kim, Suhkmann PBM-26, PES-60

Kim, Sujin PAM-10

Kim, Sun Tae GS1-8, PBM-84

Kim, Suncheun S7-2

Kim, Sung-Hwan GS3-3, GS3-8

Kim, Tae Hee PNB-108

Kim, Tae Hoon PNB-4

Kim, Tae Hwa PFS-11

Kim, Tae Jin GS4-5

Kim, Tae-Young PBM-67

Kim, Taehyun S5-2



Author Index

Kim, Taewook PBM-51

Kim, Te Ha PBM-14

Kim, Won Jun PNB-82

Kim, Won-Chan PBM-79, PBM-80

Kim, Won-Il PES-69

Kim, Woo Hee PNB-5

Kim, Woo Jung PNB-129

Kim, Yeon-Ki PBM-102

Kim, Yeong-Su PNB-42, PNB-43, PNB-44

Kim, Yi Jeong PBM-92

Kim, Yong Dae PNB-63

Kim, Yong Jun PBM-85

Kim, Yong Seok PES-1

Kim, Yong Tae PBM-40, PBM-42

Kim, Yong-Chan PNB-117

Kim, Yong-Goo PNB-33

Kim, Yong-Hak IS5-4

Kim, Yong-Suk GS4-8

Kim, Yongseok PFS-18

Kim, Youn-Chul PNB-118

Kim, Young Mee GS3-6

Kim, Young Soo PNB-49

Kim, Young-Hoon PNB-130

Kim, Young-Kee PBM-74

Kim, Youngju PNB-86, PNB-87

Kim, Youngmee PBM-11

Kim, Yu-Ji PBM-87

Kim, Yu-Na PES-29

Kishii, Masahiro PBM-8

Ko, Beom Jun PES-60

Ko, Byoung Seob PNB-22, PNB-69

Ko, Byoung-Seob PNB-19

Ko, Eun PES-37, PFS-12

Ko, Rakdo GS4-6

Ko, Yongseok PES-63

Koh, Dongsoo PNB-55

Koh, Young Ho PBM-22

Komakech, Richard PNB-33, PNB-34

Kong, Ryong GS1-3

Koo, Songyi PNB-70

Koo, Yeon Jong PES-11

Koo, Yeonjong PES-53

Koob, Michael PBM-15

Kwag, Hung-Hoon PES-20

Kwag, Jung-Hoon PES-19

Kwak, Jieun PFS-24

Kwak, Min Hyung PBM-14

Kwak, Se Yeon PFS-11

Kwak, Se-Yeon GS2-1, GS2-6, S8-3

Kweon, Haeyong PFS-14

Kwon, Deok Ho PBM-21

Kwon, Do Yeon PBM-65

Kwon, Eun-Bin PBM-20, PBM-50

Kwon, Ha-Kyong PFS-42

Kwon, Hyeon Jeong PFS-4

Kwon, Hyeyoung PBM-76, PES-7, PES-33, PES-38

Kwon, Hyung Jun PNB-119, PNB-120, PNB-121,

PNB-122, PNB-123, PNB-124,

PNB-125, PNB-126

Kwon, Hyung-Jin PBM-19

Kwon, Hyung-Joo PBM-13, PBM-14

Kwon, Jin Gwan PNB-5, PNB-6

Kwon, O Chul PBM-5, PBM-6, PNB-8

Kwon, O-Chul PFS-14

Kwon, Oh Do PES-61

Kwon, Oh-Do PES-58

Kwon, Oh-Kyung PES-69

Kwon, Seon Ju PES-46, PES-47, PES-48

Kwon, Seong Woo PBM-25

Kwon, Yonghoon S3-2

Kwon, Young-Do PNB-25

Kwon, Yu Ri PFS-38

Kyung, Kee Sung PES-7

LLe Thi, Yen PBM-27

Le, Khanh Duy PNB-53

Le, Tram-Anh Ngoc GS3-4

Lee, A Yeong PFS-34

Lee, Ah Young PFS-38, PNB-111

Lee, Ahreum GS4-6

Lee, Aro PBM-9, PBM-62

Lee, Ba Wool PNB-119, PNB-120, PNB-121, PNB-126

Lee, Byong Won GS1-8

Lee, Byung Cheon PBM-8, PBM-9, PBM-62,

PBM-63, PBM-64

Lee, Chaebok PAM-11

Lee, Chan PFS-5

Lee, Chang-Muk PBM-1, PBM-2, PBM-3

284


Lee, Cheol PBM-68, PBM-69, PBM-70,

PBM-71, PBM-72

Lee, Cho-Rong PES-17

Lee, Choon Ki PFS-24

Lee, Choong-Sik S7-3

Lee, Chul-Won IS5-4

Lee, Dae Young GS1-3, PBM-26, PNB-21,

PNB-22, PNB-36, PNB-40, PNB-77,

PNB-78, PNB-118

Lee, Dahae PNB-104

Lee, Deuk-Yeong PFS-6

Lee, Do Eun PBM-22

Lee, Dong Gun PAM-5, PAM-6, PFS-16,

PFS-17, PNB-66

Lee, Dong Sung GS3-5, PNB-19

Lee, Dong Yeol PBM-44, PES-35, PES-36

Lee, Dong-Gi PES-40

Lee, Dong-Gu PNB-71

Lee, Dong-Hwan PES-66, PES-67, PES-72

Lee, Dong-Jin PES-43

Lee, Dong-Sung PNB-118

Lee, Dongho PNB-106

Lee, Eunsong S6-2

Lee, Ga-Hyun PBM-53, PBM-78

Lee, Gang-Seob PBM-90, PBM-102, PES-4

Lee, Geung-Joo S6-1

Lee, Gi Hyun GS1-8

Lee, Gi-Hyun PBM-84

Lee, Gwan- Seok PBM-17

Lee, Gyeong Been PBM-18

Lee, Hae Min PBM-62

Lee, Han Saem PNB-47

Lee, Hanseul PBM-14

Lee, Hee Su PBM-14

Lee, Hee-Dong S8-1

Lee, Ho-Young PES-65

Lee, Hoi-Seon PNB-9, PNB-10, PNB-11, PNB-12,


Lee, Hojoung AL-2, PBM-7, PBM-8, PBM-9, PBM-10

Lee, Hoo Cheol PNB-128

Lee, Hwa-Dong PNB-32

Lee, Hwan PNB-118

Lee, Hyang-Yeol PNB-29, PNB-30

Lee, Hye Lim PBM-31

Lee, Hye Su PFS-23

Lee, Hye Suk GS2-2, S7-4

Lee, Hye-Ji PFS-42

Lee, Hye-Lime PBM-33

Lee, Hyeok PNB-55

Lee, Hyeong Hwan PBM-44

Lee, Hyeonseok PES-57

Lee, Hyeri PAM-5, PAM-6, PBM-9,


Lee, Hyo Sub PES-7

Lee, Hyo-Sub PES-45, PNB-72

Lee, Hyunyoung PBM-78

Lee, In Bog PES-39

Lee, In Chul PNB-119, PNB-120, PNB-121, PNB-122,

PNB-123, PNB-124, PNB-126

Lee, In-Jung PES-29

Lee, In-Kyoung PNB-98, PNB-99, PNB-101

Lee, Jae In PNB-122, PNB-123, PNB-124, PNB-125

Lee, Jae Pil PBM-91

Lee, Jae Won GS1-3, PBM-26

Lee, Jae-Hoon PES-44

Lee, Jaeeun PNB-3, PNB-28

Lee, Jaehak GS1-1

Lee, Jaejoon S5-2

Lee, Jechan S2-3

Lee, Jeong-Bin PNB-9

Lee, Jeong-Woo PES-51

Lee, Jeonghak GS4-6, PFS-15

Lee, Ji Eun PNB-6

Lee, Ji Hae PBM-5, PBM-6, PFS-14, PNB-8

Lee, Ji Hoon PNB-119, PNB-122, PNB-123, PNB-124

Lee, Ji Hyun PFS-5

Lee, Ji-Ho PFS-39

Lee, Ji-Hoon PAM-3

Lee, Ji-Yoon PFS-2

Lee, Jiho GS2-2, PFS-36, S7-4

Lee, Jihye PNB-127

Lee, Jihyun GS4-7, PFS-26, PFS-33

Lee, Jimin PAM-5, PAM-6, PBM-38, PBM-82,


Lee, Jin Hwan PFS-27, PFS-28, PFS-29,

PFS-30, PFS-31

Lee, Jin-Moo PNB-130

Lee, Jinwook PFS-26

Lee, Jisu PES-63

Lee, Jisun PBM-83

Lee, Jong Hwa PFS-39

Lee, Jong Rok PNB-94, PNB-96



Author Index

Lee, Jong Sik PES-27

Lee, Jong Sub PNB-122, PNB-123, PNB-124, PNB-125

Lee, Jong Suk PNB-129

Lee, Jong-Yeol GS4-1, PBM-45, PBM-46,

PBM-47, PBM-48

Lee, Jonghwa S7-4

Lee, Jongjoon S5-2

Lee, Jongsuk PBM-18

Lee, Joo Kyung PES-46, PES-47, PES-48

Lee, Jooeun PBM-79

Lee, Joon Yeop PBM-91

Lee, Ju Huck IS5-2, S5-3

Lee, Ju Sung PNB-71

Lee, Ju-Hee S2-2

Lee, Ju-Hyeon PES-44

Lee, Jun PNB-106

Lee, Jun-Young S4-1

Lee, Jung A PNB-5, PNB-6, PNB-129

Lee, Jung-Sook IS5-2

Lee, Junghak GS2-2, PFS-39, PFS-41, S7-4

Lee, Jungwhoi PBM-69, PBM-72

Lee, Junho PBM-23, PBM-24, PES-9, PNB-2,

PNB-3, PNB-26, PNB-27, PNB-28, S1-2

Lee, Ka Yeon S6-1

Lee, Keun Chul IS5-2

Lee, Ko-Eun PES-29

Lee, Kwang Sik PNB-85

Lee, Kwang-Sik PNB-80

Lee, Kwanghun PFS-36

Lee, Kyounghoon PNB-114

Lee, Kyuhong GS3-3, GS3-8

Lee, Kyung Dong PNB-86, PNB-87

Lee, Kyung-Ha S1-1

Lee, Kyung-Tae PNB-106

Lee, Kyungha PBM-4, PBM-56

Lee, Mi Ja PNB-85

Lee, Mi Jeong PFS-9, PFS-10

Lee, Mi Kyeong PBM-50

Lee, Mi-Ae PAM-10

Lee, Mi-Ja PNB-79, PNB-80, PNB-84

Lee, Min-Ki GS4-4, GS4-8, S4-3

Lee, Min-Sung PNB-42

Lee, Mun Hyon PES-2

Lee, Na-Hyun PAM-5, PAM-6, PFS-16, PNB-66

Lee, Ok Ran GS1-6, PBM-55

Lee, Rheeda PFS-32

Lee, Saet Buel PNB-108

Lee, Sang Gu PFS-7

Lee, Sang Gyu PAM-5, PAM-6, PFS-16, PFS-17, PNB-66

Lee, Sang Hoon S4-3

Lee, Sang Hyeob PFS-11

Lee, Sang Hyuk GS1-3

Lee, Sang Ryong PES-65, PES-75

Lee, Sang Woo GS3-2

Lee, Sang-Bum S8-1

Lee, Sang-Hoon GS4-4

Lee, Sang-Hyeob GS2-1, GS2-6

Lee, Sang-Hyeon PES-2

Lee, Sang-Hyun PFS-35

Lee, Sang-Min PES-17

Lee, Sang-Ryong PES-71

Lee, Sang-Won PAM-10

Lee, Sanghwa PBM-61

Lee, Sanghyun PNB-71, PNB-109, PNB-115

Lee, Sangyool PBM-12

Lee, Sarah PNB-97

Lee, Seo Wk PNB-61

Lee, Seoghyen PBM-50

Lee, Seok-Min PES-29

Lee, Seokjin AL-2, PBM-10

Lee, Seon Mi PES-61, PES-62

Lee, Seon-Hye GS4-4, GS4-8, S4-3

Lee, Seon-Kyeong PBM-3

Lee, Seong Eun PES-39

Lee, Seong Mi GS3-2, PNB-82

Lee, Seung Ju PFS-1

Lee, Seung Sik PBM-36, PBM-49, S3-1

Lee, Seung Woong PNB-58, PNB-59

Lee, Shinhui PNB-27

Lee, Si Hyeock PBM-21, PBM-22

Lee, Si Myung PFS-7

Lee, Sohee GS1-2

Lee, Song Min PES-2

Lee, Songhee PES-37

Lee, Soo Chan S5-1

Lee, Su Ha PNB-98

Lee, Su In PBM-13

Lee, Su Jin PBM-30, PNB-46

Lee, Su Ui GS3-2, PBM-20, PBM-50, PNB-89

Lee, Su-Bin PBM-47

Lee, Su-Lim PES-44

Lee, Suji GS4-4, GS4-8, S4-3

286


Lee, Sun Hee PNB-91

Lee, Sun Il PES-27

Lee, Sun-Mi PFS-2

Lee, Sung Hyun PNB-90

Lee, Sung-Eun GS2-5, PES-41, PES-42, PES-64,

PES-65, PFS-40, PNB-117

Lee, Sungbeom PBM-36, S3-1

Lee, Tae Hyun PES-49

Lee, Taegum PBM-30

Lee, Theresa PFS-9, PFS-10

Lee, Won Je AL-2, PBM-7, PBM-8, PBM-9, PBM-10

Lee, Wonho PES-60

Lee, Woo Song PNB-126

Lee, Yeong-Geun GS3-5, PNB-19, PNB-20,

PNB-21, PNB-22

Lee, Yeonseon S5-1

Lee, Yong-Sang PES-18

Lee, Yoon-Yeol S4-1

Lee, Youn-Hyung GS3-5, PNB-19, PNB-20,


Lee, Young Bo PFS-14

Lee, Young Han PES-54, PNB-55

Lee, Young-Seob GS1-3, PBM-26, PNB-36,

PNB-77, PNB-78

Lee, Younghee PBM-13, PBM-14

Lee, Youngshim PNB-55

Leem, Hyun Hee PBM-91

Li, Qing X. IS2-1

Li, Wan-Yi GS3-2

Li, Zuo Peng PNB-17

Lim, Hee Ju PNB-59

Lim, Jeong-Hyeon PBM-81

Lim, Ju-Yeon PES-66, PES-67

Lim, Ki Hwan PBM-80

Lim, Min Ju PNB-122, PNB-123, PNB-124, PNB-125

Lim, Myung-Ho PES-3

Lim, Sangyong S5-1

Lim, Sun-Hyung PBM-45, PBM-46, PBM-47, PBM-48

Lim, Sung-Jin PES-45, PNB-72

Lim, Yoongho PNB-55

Lim, Yoonji PBM-19

Lim, Yu Mi PNB-47

Liu, Kwang-Hyeon PBM-53, PBM-66, PBM-78

Liu, Qingzhen PNB-92, PNB-93

MMa, Seohee PBM-26

Madusanka, Disanayaka GS4-9, PNB-113, PNB-114

Mudiyanselage Dinesh

Maeng, Jihye S3-3

Maharjan, Sony PBM-13

Makhabbat, Zhelderbayeva IS4-2

Mani, Vimalraj PBM-1, PBM-2, PBM-3

Matsabisa, Motlalepula Gilbert PNB-34

Meng, Hui Wen PNB-111

Min, Cheol Woo GS1-8, PBM-84

Min, Sang-Kee PFS-2

Min, Seul Ki PNB-128

Mishra, Awdhesh PAM-7

Mok, Hyejung IS3-3

Moon, Byeong Cheol PNB-106

Moon, Byeong-Chul PBM-76, PES-7, PES-33, PES-38

Moon, Dong-Oh PBM-20

Moon, Jeong Yong PBM-35

Moon, Joon Kwan PFS-25

Moon, Joon-Kwan PES-49

Moon, Youngkook PBM-41, PBM-99

Moustaid-moussa, Naima IS3-1

Mun, Ye Ji PES-12, PES-13, PES-14, PES-15,

PES-16, PES-30, PES-31, PES-32

Muthuramalingam, Karthika GS3-6, PBM-11

Mydul Islam, A. K. M. PBM-76, PES-38

NNa, Hyeon Seon PNB-78

Na, Yung Ran PFS-4

Nah, Ju-Young PFS-10

Nahm, Baek Hie PBM-90, PBM-102

Nam, Ae Ji PFS-11

Nam, Ae-Ji GS2-1, GS2-6

Nam, Gaewon S3-3

Nam, Gi-Hum PNB-97

Nam, Heejin PBM-60

Nam, Hongshik PES-56

Nam, Mi Na PFS-38

Nam, Sang Hee PFS-30, PFS-31



Author Index

Nam, Seungah PBM-83

Nam, Yoon Hee PNB-22

Nam, Youn Hee PNB-21

Namgoong, Hwan PBM-27

Nan, Li PNB-100, PNB-102, PNB-103

Nguyen, Hoa Thi PNB-53

Nguyen, Khoa PBM-23

Nguyen, Thu Thi GS4-3, PFS-21

Nguyen, Van Thi PNB-53

Nimchua, Thidarat PAM-1

Nogrado, Kathyleen PAM-3

Noh, Hye-Lyeong PBM-81

Noh, Hyeon Ji PES-6

Noh, Hyun Ho PBM-76, PES-7, PES-38

Noh, Jeong Sook PNB-115

OOh, Byeong Seob IS5-2

Oh, Dae-Ju PBM-68

Oh, Eun Sol GS3-2, PNB-89

Oh, Eunji PBM-97, PBM-101

Oh, Heejeong PES-53

Oh, Hyun-Ji PNB-22

Oh, Hyuncheol PNB-118

Oh, Je Jun PES-9, S1-2

Oh, Joa Sub PNB-5, PNB-6

Oh, Jong Min PBM-92

Oh, Jongmin PBM-91

Oh, Min-Seok PBM-76, PES-7, PES-33, PES-38

Oh, Nuri PBM-38, PBM-82

Oh, Sei Ryang PNB-89

Oh, Sei-Ryang GS3-2, PBM-20, PBM-50,

PNB-81, PNB-82

Oh, Seon Min PNB-40, PNB-78

Oh, Seon Woo PFS-7

Oh, Suk Gui PBM-85

Oh, Sukgui PBM-93, S6-2

Oh, Sung-Dug PES-3, PES-4

Oh, Ui Hyeon PBM-68, PBM-70, PBM-71

Oh, Ui-Hyeon PBM-69, PBM-72

Oh, Young Gon PES-40

Oh, Young Ju PBM-100

Oh, Yu Jin PNB-42

Oh, Yu-Jin PNB-43, PNB-44

Oh, Yura PES-17

Ok, Chul-Ho GS3-7

Ok, Younju PFS-3

Okabe, Satoshi IS5-1

Okello, Denis PNB-34

PPahk, Yoonmok PBM-102

Park, Ae Ran GS2-3, PES-34, PNB-53, PNB-54

Park, Bueyong PBM-17

Park, Byoung Kwon PBM-13

Park, Chan Hum PNB-7, PNB-110

Park, Chang Eon PBM-58

Park, Chang-Wook PES-29

Park, Choong-Bae PES-56

Park, Daeui PBM-91

Park, Do Hwi PNB-104

Park, Dongu-Ju PFS-2

Park, Eun Mi PBM-39

Park, Eun-Jae PNB-59

Park, Eun-Jung PES-29

Park, Eunsun PBM-27

Park, Eunyoung GS2-2, GS4-6, PFS-41, S7-4

Park, Gwang Hun PNB-64, PNB-65

Park, Hae Woong GS2-3, PES-34, PNB-54

Park, Hee Sung PNB-31

Park, Hee-Jung PFS-35

Park, Hee-Soon PES-29

Park, Ho Jeong PFS-29, PFS-30, PFS-31

Park, Hye Lin PBM-56

Park, Hye Yoon PNB-92, PNB-93, PNB-95, PNB-97

Park, Hye Young PFS-24

Park, Hye-Bin PES-29

Park, Hye-Jin PFS-42

Park, Hye-Jung PES-2

Park, Hyeon-Jin PES-73

Park, Hyo-Hyun PNB-37

Park, Hyun Jun PES-46, PES-47

Park, Hyun-Jin PES-66, PES-67, PES-70, PES-72

Park, In-Dal GS3-7

Park, Jae-Hyeok PES-73

Park, Jam-Eon IS5-2, S5-3

288


Park, Ji Young PNB-120, PNB-121, PNB-122, PNB-123,

PNB-124, PNB-125, PNB-126

Park, Ji-Eun GS4-2, PFS-19, PNB-37

Park, Jieun PFS-22

Park, Jihyun PNB-55

Park, Jin Hee PES-46, PES-47, PES-48

Park, Jin Woong PES-61

Park, Jina PBM-97, PBM-101

Park, Jong Eel PNB-45, PNB-105

Park, Jong-Hwan PES-5, PES-44, PES-51

Park, Joo-Seok PBM-52, PBM-54, PBM-57

Park, Joongwon PBM-14

Park, Joonho PBM-27

Park, Jun Oh PNB-46

Park, Jun-Hwan PNB-12, PNB-13

Park, Jun-Kyung IS5-4

Park, Jung Soo PNB-57

Park, Kee-Choon PES-56

Park, Keon-Tae PNB-56

Park, Ki Hun PNB-16, PNB-17, PNB-18

Park, Kwang Soo S6-1

Park, Kwang-Lai PES-56

Park, Kyu Hee PNB-129

Park, Man-Seong PBM-13

Park, Mi Hyeon PNB-81

Park, Min-Kyu GS2-5, PES-65

Park, Minjeong PNB-38, PNB-39

Park, Nam Il GS4-5

Park, Sang Heyong PAM-7, PES-52

Park, Sang Hun PNB-52

Park, Sang Un PBM-65, PNB-83

Park, Sang kyu PBM-14

Park, Se-In PES-66, PES-67, PES-70, PES-72

Park, Seong Jin PES-24, PES-59, PES-68

Park, Seong-Jin PES-10

Park, Seung Bin PNB-122, PNB-123, PNB-124

Park, Seung-Hwan IS5-2, S5-3

Park, So-Young PBM-66, PBM-78

Park, Sohyun PFS-3

Park, Soo Yun PFS-7

Park, Soo-Yun PES-3

Park, Sook Jahr PNB-94, PNB-96

Park, Soon Ju PBM-85, PBM-93, S6-2

Park, Soyoung PBM-1, PBM-2, PBM-3

Park, Suhyun GS1-7, PNB-107, PNB-108

Park, Sujin PBM-98, PES-74

Park, Sung Ah PFS-4

Park, Sung-Chul PBM-86, PNB-108

Park, Tae Kwon PES-28

Park, Taehyeon PBM-51

Park, Woo-Ram PBM-88

Park, Ye-Ji PNB-58

Park, Yeong-Jun PBM-58

Park, Yon Koung PFS-4

Park, Yong Dae PNB-51, S3-1

Park, Youngki PNB-64

Park, Yun Ji PNB-83

Pathiraja, Duleepa PBM-9

Petrov, Max S. IS4-1

Phong, Huynh Xuan IS4-3

Prakash, Prapti PBM-36

Priatama, Ryza A. S6-2

Priatama, Ryza Aditya PBM-93

Pyun, Bo-Jeong PNB-69

Pyun, Jang Won PBM-86, PNB-108

QQi, Yutong PNB-56

Quang Pham, Huy PAM-4, PBM-58

RRa, Ji Eun PNB-84, PNB-85

Ra, Ji-Eun PNB-79, PNB-80

Rabbee, Muhammad Fazle PAM-9

Rahman, M. Mokhlesur IS2-2

Rahmat, Endang PNB-35

Rajendran, Sujeevan PBM-93

Reddy, Chinreddy Subramanyam PBM-1, PBM-2, PBM-3

Rehan, Mai GS2-2, GS4-6, PFS-41

Rhee, Jeewon PES-45, PNB-72

Rho, Jin-Ho PFS-6

Rho, Mun-Chual PNB-58, PNB-59

Riu, Key-Zung PBM-73

Ro, Hyunju PNB-89

Ro, Jin-Ho PBM-76, PES-7, PES-33, PES-38, PFS-8

Roh, Yeon Jin PBM-63



Author Index

Ryu, Chang Hwan GS1-4

Ryu, Hyung Won GS1-8, GS3-2, PBM-20, PBM-50,


Ryu, Jun Sun GS1-4

Ryu, Seung Mok PNB-106

Ryu, Song-Hee PES-45, PNB-72

Ryu, Songhee PFS-15

Ryu, Tae Hoon PFS-7

Ryu, Young Bae PNB-119, PNB-120, PNB-121, PNB-122,

PNB-123, PNB-124, PNB-125, PNB-126

Ryuk, Jin Ah PNB-19

SSaranchimeg, Munkhbat PFS-21

Sarker, Aniruddha GS2-1, GS2-6, PFS-11

Schulthoff, Saskia S3-2

Selvakumar, Gopal PES-39

Seo, Bo-Seong PES-66, PES-67

Seo, Bo-Sung PES-72

Seo, Byung Hwan PES-50

Seo, Chang-Seob PFS-34

Seo, Changon PBM-18, PNB-5, PNB-6, PNB-129

Seo, Dong Cheol PES-55

Seo, Dong-Cheol PES-5, PES-44, PES-51

Seo, Eunyoung PBM-51

Seo, Ho Seong S5-1

Seo, Jang-Kyun PBM-28

Seo, Jeong Won PES-11

Seo, Jeong-A PFS-23

Seo, Jin Hwan PNB-86, PNB-87

Seo, Mi Suk PNB-129

Seo, Si Young PES-19, PES-20

Seo, Sun-Il IS5-4

Seo, Woo Duck PNB-84, PNB-85

Seo, Woo-Duck PNB-79, PNB-80

Seo, Yeongwan PNB-39

Seo, Young Hye PNB-106

Seo, Youngwan PNB-38

Seok, Young Mi PNB-45

Seol, Jae Ung PES-40

Seon, Ho Young PNB-63

Seon, Jeong Hoon PFS-25

Seong, Hyeon Jeong PES-6

Shah, Abdul Bari PNB-17

Shanmugam, Gnanendra S5-2

Sharma, Chanchal GS1-5

Shim, Kyo Moon PES-1

Shim, Kyo-Moon PFS-18

Shim, Yong Kun PES-9

Shim, Young Kun S1-2

Shin, Chanseok GS1-9, PBM-51

Shin, Dong Won PES-60

Shin, Eun Ji GS4-9

Shin, Eun-Ji PNB-112, PNB-113, PNB-114

Shin, Han Gyo PNB-119, PNB-120, PNB-121, PNB-126

Shin, Heungsop PBM-40, PBM-42, PBM-83

Shin, Hwa Sung PNB-128

Shin, Hy Bin PNB-63

Shin, Hye Lim PNB-85

Shin, Hye-Lim PNB-79, PNB-80

Shin, Jae-Ho PAM-4, PBM-58

Shin, Ji-Sun PNB-106

Shin, Jihyun PFS-32

Shin, Jisoon PNB-130

Shin, Jong Hoon GS3-1

Shin, Min Ki PFS-20

Shin, Myoung Nim S4-2

Shin, Soo Im PNB-67

Shin, Sooim PBM-41, PBM-59, PBM-60,

PBM-99, PES-37, PFS-12

Shin, Soon Young PNB-55

Shin, Yongho GS2-2, PFS-39, S7-4

Shin, Yu Su PNB-7, PNB-110

Sim, Eunyeong PFS-24

Sim, Geun Yong PBM-37

Sim, Geun Young PBM-30

Sim, Jae-Ryeong PBM-48

Sim, Joon-Soo PBM-1, PBM-2, PBM-3

Sim, Soon Ae PAM-8

Sin, Seung Mi PBM-44, PES-35, PFS-38

Singh, Ashutosh S1-3

So, Jai Hyun PNB-47

So, Jai-Hyun PNB-37

Sohn, Soo-In PBM-100

Son, Eun Kyung PNB-47

Son, Eun-Kyung PNB-37

Son, Hyun-Woo PAM-4

Son, Jae Kwon PES-21, PES-22, PES-23,

PES-25, PES-26

290


Son, Jun Ho PNB-105

Son, Kyunghun PFS-32

Son, So-Ri PNB-56

Son, Soojeong PFS-32

Son, Youn Kyoung PNB-95

Son, Yun PBM-75

Song, Bong Kyu PNB-93

Song, Bongkyu PNB-92, PNB-95

Song, Byung-Hun PES-43

Song, Eun-Ju PFS-2

Song, Gwanpil PNB-91

Song, Hyuk-Whan PES-69

Song, Jae Do PES-21, PES-22, PES-23,

PES-25, PES-26

Song, Jaekyeong IS5-4

Song, Ji Hoon PNB-104

Song, Kyeong Yeong PNB-86, PNB-87

Song, Min Kyung PBM-30

Song, Woo-Young PBM-67

Song, Young Hun PBM-94

Sonn, Yeon Kyu PES-50

Soon, Yoo Ju PBM-84

Subburaj, Saminathan S6-1

Suh, Min Kuk IS5-2

Suh, Soo Kyung PNB-130

Sun, Jeong Hoon PES-49

Susanto, Tan Steven Ryan PNB-116

Suwanprateep, Juthamas PAM-1

TTasho, Reep Pandi PES-12, PES-13, PES-14,

PES-15, PES-16, S2-1

Thanonkeo, Pornthap IS4-3

Thi Hai Duong, Doan PBM-77

Thongkred, Paweena PAM-1

Torii, Keiko U. IS1-2

Truong, Hai An AL-2, PBM-8, PBM-10

Trịnh, Cao Son PBM-9

Trịnh, Cao Sơn AL-2, PBM-10

Tu, Luhua S6-1

UUm, Jae-In PNB-70

Um, Min Young PFS-12

Um, Min-Young PNB-67

Um, Taeyoung PBM-51

VVan Vu, Tien PBM-77

Van, Tien Do PES-65

WWee, Ji-Hyang PNB-95

Wirtz, Conny S3-2

Woo, Dong Geon PNB-71

Woo, E-Eum PNB-98, PNB-99, PNB-101

Woo, Hyun Sim PNB-42, PNB-43, PNB-44

Woo, Ji Eun PBM-25

Woo, Saem-Ee PES-19, PES-20

XXi, Jinying PES-75

YYamada, Mamoru IS4-3

Yang, Chang Yeol PNB-7

Yang, Doog-Hoon S5-1

Yang, Hye In PES-70, PES-72

Yang, Hye-In PES-66, PES-67

Yang, Hyeok Jun PFS-13

Yang, Jungeun PBM-18

Yang, Kwang Yeol PNB-108

Yang, Seung-Hyun GS2-4



Author Index

Yang, So hee PES-53

Yang, Soo-Kyung PNB-91

Yang, Young SL-1

Yang, Youri PBM-67, PES-63

Yang, Yu-Mi PNB-58

Yeo, In-Seok PAM-2

Yeo, Joo Hong PNB-53

Yeo, Joo-Hong PBM-22

Yeob, So Jin PES-54

Yeon, Jehyeong GS2-3, PES-34

Yi, Pyoung Ho PES-39

Yi, Tae Gyu GS4-5

Yim, Mijin PNB-1

Yim, Soo Kwang PBM-55

Yim, Soobin PFS-10

Yim, Soon Ho PNB-61, PNB-63

Yim, Soon-Ho PNB-62

Yokozawa, Takako PNB-7

Yong, Ye-Seul PNB-62

Yoo, Dong Gon PES-8

Yoo, Hye Hyun PNB-130

Yoo, Jeong-Kyun PNB-49

Yoo, Ji Hyock PES-62

Yoo, Ji-Hyock PES-58, PES-61, PES-69

Yoo, Miri PNB-55

Yoo, Seon Mi GS4-4, S4-3

Yoo, Youngchul PAM-10

Yoon, Dahye PBM-26, PNB-40, PNB-77,

PNB-78, PNB-118

Yoon, Ho Young PNB-73

Yoon, Hyeokjun PNB-53

Yoon, Hyoin PES-45, PNB-72

Yoon, Jeongyeon PBM-51

Yoon, Ji Hyun PES-61

Yoon, Ji-Hyun PES-58

Yoon, Kwang-Sik PES-72

Yoon, Kyungjae Andrew PBM-22

Yoon, Yongdae PNB-23

Yoon, Young Geol PBM-15

You, Han-Nui PFS-2

Youn, Jong Bae PFS-1

Yu, Hyejin PES-45, PFS-15, PNB-72

Yu, Nan Hee GS2-3, PES-34, PNB-53, PNB-54

Yu, Seung Yeob IS5-2

Yu, Song Yi PNB-69

Yu, Yeon Su PAM-7, PES-52

Yuan, Xiu GS4-6, PFS-41

Yun, Bong-Sik PNB-98, PNB-99, PNB-101

Yun, Doh-Won PES-3, PES-4

Yun, Seong Ho PES-8

Yun, Sun Gang PES-50

Yun, Yeong-Bae PBM-74

ZZhu, Yong- Zhe PBM-17

Zhumanova, Kamila PNB-16

발행일 2019년6월20일

발행처 (사)한국응용생명화학회

서울시강남구테헤란로7길22한국과학기술회관신관803호

Tel. 02-568-0970,02-568-0799(학술지)

Fax. 02-568-0971

Homepage http://www.ksabc.or.kr

E-mail. [email protected] / [email protected]

디자인 및 인쇄 씨디엠더빅

서울금천구디지털로9길32,갑을그레이티밸리B동1805호

Tel. 02-857-1573

Fax. 02-857-1574

E-mail. [email protected]

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