Posters 1. SYSKA Camilla VapBC Toxin Antitoxin systems : actors of the nitrogen-fixing symbiosis. 2. CARRÉ-MLOUKA Alyssa Signalling molecules involved in actinorhizal symbioses. 3. SVISTOONOFF Sergio Using wild Arachis species and Senegalese rhizobial strains to improve

biological nitrogen fixation of cultivated peanut in Senegal. 4. CAPELA Delphine Multiple genetic routes to improve nodule cell infection during experimental

evolution of legume symbionts. 5. LUU Thi Bich LYK cluster variations and Symbiotic interactions in Medicago truncatula. 6. BARRIÈRE Quentin Into the search of microsymbiont factors involved in NCR adaptation during

Sinorhizobium – Medicago symbiosis. 7. BUSSET Nicolas A multi-omics approach to identify type III effectors of the Bradyrhizobium

strain ORS3257. 8. NICOUD Quentin Metabolic modeling of Bradyrhizobium sp ORS285 as a tool to understand its

versatile lifestyles. 9. WALLNER Adrian Adaptation des Burkholderia à différents types d'interactions avec le riz:

approches génomiques et transcriptomiques. 10. BRAULT Mathias Diversification of cytokinin phosphotransfer signaling genes in Medicago

truncatula. 11. GRUBER Véronique Defense versus senescence mechanisms in Medicago truncatula nodules. 12. VERNIÉ Tatiana Evolution and Re-evolution of symbiotic gene regulatory networks. 13. MOHAMAD Roba Cross-selection by two legume genera of the Loteae Tribe (Anthyllis and Lotus)

of their rhizobial partners from soils

Access plan The meeting will be held at Agropolis International 1000, Avenue Agropolis F-34394 Montpellier Cedex 5 (France) GPS : N 43.645935 E 3.869505 A free car park is available next to the building.

Supported by

Organized by Eric GIRAUD, Lionel MOULIN and Valérie HOCHER

6th Meeting on Molecular

Mechanisms in Nitrogen-

Fixing Root Endosymbioses

Montpellier September 19-20

2019 Infos sur le site :

https://valeriehocher.wixsite.com/monsite/

Agropolis International

IRD

Parking

Organizing committees

Program Thursday, 19th September

13:30 Meeting introduction Lionel Moulin, Eric Giraud, Valérie Hocher 14:00 - 15:15 Session 1. Chairperson: Pierre-Marc DELAUX 14:00 - 14:15 Delphine CAPELA (LIPM, Toulouse) Multiple genetic routes to improve nodule cell infection during experimental evolution of legume symbionts. 14:15 - 14:30 Jean KELLER (LRSV, Toulouse) Phylogenomic and phylogenetic to decipher Evolution of Nitrogen-Fixing Root-Nodule Symbiosis. 14:30 - 14:45 Pascal RATET (IPS2, Paris Saclay) Ensifer adhaerens T4, a patho-symbiote for Medicago. 14:45 - 15:00 Benjamin GOURION (LIPM Toulouse) Tripartite systems to explore causes and consequences of nodule immunity 15.00 – 15.15 Pongpan SONGWATTANA (U. Suranaree, Thaïlande) Symbiotic properties of a chimeric Nod-independent photosynthetic Bradyrhizobium strain obtained by conjugative transfer of a symbiotic plasmid.

15:15 - 16:00 Coffee break

16:00 - 17:00 Session 2. Chairperson: Peter MERGAERT 16:00 - 16:15 Andreas NIEBEL (LIPM Toulouse): A study of the symbiotic Medicago truncatula Sty/Shi genes.

16:15 - 16:30 Quentin BARIERRE (I2BC, Paris Saclay) Into the search of microsymbiont factors involved in NCR adaptation during Sinorhizobium – Medicago symbiosis. 16:30 - 16:45 Hasna BOUBAKRI (EM, Lyon) Alnus glutinosa upregulated peptides: effects on its bacterial symbiont Frankia. 16:45 - 17:00 Emanuele BIONDI (LCB, Marseille) Regulation of bacterial cell cycle during nitrogen fixing symbiosis. 17:00 - 17:15 Guilhem DESBROSSES (BPMP, Montpellier) Different inoculation protocols unravels the complexity of the signalling between the nitrogen fixing rhizobium Mesorhizobium loti and the model plant Arabidopsis thaliana.

17:15 - 19:15 Poster session and aperitif 20:15 Dinner in Montpellier (down town)

Friday, 20th September

08:45 Welcoming 9:00 – 10:15 Session 3. Chairperson: Clare GOUGH 9:00 – 9:15 Martin CRESPI (IPS2, Paris Saclay) Small RNA-mediated regulations in the legume rhizobium symbiosis 9:15 – 9:30 Johan QUILBE (LSTM, Montpellier) Forward genetics in Nod-independent Aeschynomene species reveal conservations and variations in the signaling pathway and allow the identification of a new specific Receptor-Like Cytoplasmic Kinase. 9:30 – 9:45 Mame Diarra MBENGUE (LIPM, Toulouse) A mutation in E3 ubiquitin ligase PUB1 alters nod factor structure recognition in Medicago truncatula. 9:45 – 10:00 Tatiana VERNIE (LRSV Toulouse) Evolution and Re-evolution of symbiotic gene regulatory networks 10:00 – 10:15 William Patrick BUHIAN (LIPM, Toulouse) Functional characterization of MtIAA7, a putative regulator of lateral root development in Medicago truncatula.

10:15 – 11:00 Coffee break

11:00 – 12:15 Session 4. Chairperson: Jacques BATUT 11:00 – 11:15 Nico NOUWEN (LSTM, Montpellier) The modification of the flavonoid naringenin by Bradyrhizobium sp. ORS285 converts the nod genes inducer function to a growth stimulator 11:15 – 11:30 Geneviève ALLOING (Sophia Antipolis, Nice) A bacterial fluorescent biosensor to analyse changes in redox conditions during S. meliloti/M. truncatula symbiosis 11:30 – 11:45 Quentin NICOUD (I2BC, Paris Saclay) Metabolic modeling of Bradyrhizobium sp ORS285 as a tool to understand its versatile lifestyles. 11:45 – 12:00 Anne-Claire CAZALE (LIPM, Toulouse) Nitric oxide triggers tyrosine nitration of Sinorhizobium meliloti proteins 12:00 12:15 Alexandre BOSCARI (Sophia Antipolis, Nice) Involvement of group VII Ethylene Response Factors in oxygen sensing and adaptation to hypoxia in roots and nodules of Medicago truncatula

12:15 – 14:00 Lunch break Salle Vanille

14:00 - 15:00 Session 5. Chairperson: Pascal RATET 14:00 - 14:15 Florian FRUGIER (IPS2, Paris Saclay) Local and systemic regulation of symbiotic nodulation. 14:15 - 14:30 Eoghan KING (IPME, Montpellier) Comparative analysis of rice sensing of plant-beneficial Burkholderia. 14:30 – 14:45 Arlette ZAIYA ZAZOU (IRAD, Cameroun) Using wild peanut species for improving native biological nitrogen fixation in rhizobia symbiosis.

14:45 – 15:15 Session 6. Chairperson Catherine Masson-Boivin Concluding remarks & discussion on future actions of SymbiFix.

Scientific Pierre FRENDO Fernanda DE CARVALHO-NIEBEL Eric GIRAUD Catherine MASSON Pierre-Marc DELAUX

Local Eric GIRAUD Valérie HOCHER Lionel MOULIN Clotide SABOT Nathalie CLAUDE

Peter MERGAERT Hasna BOUBAKRI Pascal RATET Lionel MOULIN Valérie HOCHER Florian FRUGIER