young cell 的 分佈
DESCRIPTION
Young Cell 的 分佈. Aty-Lym. Fluorescence (RNA ,DNA). ProMyelo. Myelo. Blast. Meta. Band. Resistant RBC 既沒有 RNA, 也沒有顆粒 , 因此即使有 紅血球破壞不全的現象 , 也不會干擾 WBC 的分類. RBC Ghost. Side Scatter ( 內容物結構 ). Estimation of cell viability by flow cytometry. (Death cells). (Live cells). - PowerPoint PPT PresentationTRANSCRIPT
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Young Cell Young Cell 的 分佈的 分佈
Side Scatter ( 內容物結構 )
Band
Meta
MyeloProMyelo
Blast
Aty-Lym
Fluo
resc
ence
(R
NA
,DN
A)
Resistant RBC 既沒有RNA,也沒有顆粒 , 因此即使有紅血球破壞不全的現象 , 也不會干擾 WBC 的分類
RBC Ghost
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Estimation of cell viability by flow Estimation of cell viability by flow cytometrycytometry
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Protocol for viability
細胞培養之細胞數約細胞培養之細胞數約 1×101×1055~2×10~2×1055//wellwell ,加入不同濃度藥物,培養,加入不同濃度藥物,培養不同的時間不同的時間
↓↓收細胞收細胞↓↓
加入螢光染劑(加入螢光染劑( PI for viabilityPI for viability ))↓↓
流式細胞儀分析流式細胞儀分析
(Death cells)
(Live cells)
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Cell cycle analysis
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Protocol for cell cycleProtocol for cell cycle
細胞培養之細胞數約細胞培養之細胞數約 1×101×1055~2×10~2×1055/well/well ,加入不同濃度藥物,培養不同的時間,加入不同濃度藥物,培養不同的時間↓↓收細胞收細胞↓↓加入加入 70% ethanol70% ethanol ,固定細胞。放入,固定細胞。放入 -20-20 度冰箱儲存,至少度冰箱儲存,至少 2424 小時。小時。↓↓
將將 -20-20 度中的度中的 samplesample 從冰箱取出從冰箱取出↓↓離心離心 1500rpm1500rpm ,, 5mins5mins↓↓倒掉上清液,並打散細胞倒掉上清液,並打散細胞↓↓
PBS washPBS wash↓↓
1500 rpm1500 rpm ,, 5mins5mins↓↓加入加入 PIPI (( for cell cyclefor cell cycle )作用)作用 3030 分鐘分鐘↓↓流式細胞儀分析流式細胞儀分析
☆ ☆ cell cyclecell cycle 所用的所用的 PIPI 與與 viabilityviability 所用的所用的 PIPI 成分不同 成分不同
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G0/G1
S
G2/M
Apoptosis
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G2/M
G2/M
G2/MG2/M
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G0/G1G0/G1
G0/G1
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Analysis of apoptosis by flow Analysis of apoptosis by flow cytometrycytometry
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Phosphatidylserine (PS) and apoptosis
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Apoptosis rate analysis using Annexin V/propidium iodide flow cytometry in cancer cells treated with drug
Viable cellsEarly stage of apoptosis
Later stages of apoptosis and necrosis
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Apoptotic cells
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Detection of intracellular/intranuclear Detection of intracellular/intranuclear antigensantigens
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Detection of intracellular/intranuclear antigensDetection of intracellular/intranuclear antigens
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Tumor Antigen Markers