what is the baseline susceptibility of aphids to transform and sivanto prime?
TRANSCRIPT
What is the Baseline Susceptibility of Aphids to Transform and Sivanto?
David Kerns, Sebe Brown & John David GonzalesLouisiana State University AgCenter
Sorghum – Sugarcane Aphid Research Exchange Meeting
New Orleans, LAJanuary 7 – 8, 2016
CollaboratorsName Affiliation Trial Location 2014 2015Gus Lorenz University of Arkansas Rowley & Marianna, AR July Aug
Michael Brewer Texas A&M University Corpus Christi, TX July June
Kathy Flanders Auburn University Clanton & Macon Co., AL Aug June
Amanda Jacobson Auburn University Brewton, AL -- July
Tom Royer Oklahoma State University Lane & Garfield Co., OK Aug Aug
Stephen Biles Texas A&M University Port Lavaca, TX Aug --
Scott Stewart University of Tennessee Madison Co., TN -- Aug
Danielle Sekula Texas A&M University Weslaco, TX Aug June
Angus Catchot Mississippi State University Starkville & Grenada, MS Aug July
Nick Seiter University of Arkansas McGehee, AR -- Aug
David Buntin University of Georgia Pike Co., GA -- Oct
Drew Higgins Higgins Farms Castro Co., TX -- Aug
Francis Reay-Jones Clemson University Florence, SC -- July
Shelby Williams Ag-Venture Seed Poinsett Co., AR -- July
Kerns, Brown & Gonzales
Louisiana State University Alexandria, Jonesville, Winnsboro, St Landry &
Somerset, LA
July, July, Aug
Jun, Jun, Aug
Introduction
• Research will establish early baseline data on key insecticides used to manage sugarcane aphid.
• Baseline data will be used to benchmark insecticide efficacy so shifts in susceptibility can be later detected in subsequent years.
Materials & Methods• Bioassays with Transform in 2014 & 2015, and Sivanto
in 2015.• Utilized a leaf dip method.
– Insecticide concentrations tested included: 0.0, 0.5, 2, 5, 10, 15, 25, and 35 ppm active ingredient.
– Included Induce non-ionic surfactant at 0.5% v/v.• 8 replicates per dose
• Prepared 29-ml condiment cups with 2 ml of agar.• Cut 3-cm diameter leaf discs placed in the cups abaxial
side up.• Placed a steel washer over the leaf.• Placed 10 adult SCA on the exposed leaf in each cup.• Sealed with single ply tissue.• Placed in growth chamber at 27 ± 1° C for 48 H.
Procedures
Sulfoxaflor 2014
Alexandria, LA
Port Lavaca, TX
Weslaco, TX
Jonesville, LA
Starkville, MS
Corpus Christi, TX
Lane, OK
Clanton, AL
Rowley, AR
Winnsboro, LA
mean median
0
2
4
6
8
10
12
14
16
Sulfoxaflor (LC50
-ug/ml)
Sulfoxaflor 2015
Castro County, TX
Garfield County, OK
Florence, SC
Grenada, MS
Pike County, GA
Poinsett County, AR
Madison County, TN
Marianna, AR
Somerset, LA
Corpus Christi, TX
Macon County, AL
St. Landry Parish, LA
Brewton, AL
Weslaco, TX
Winnsboro, LA
Mean Median
0
2
4
6
8
10
12
14
16
Sulfoxaflor (LC50
-ug/ml)
Flupyradifurone 2015
Castro County, TX
Florence, SC
Weslaco, TX
Grenada, MS
Pike County, GA
Brewton, ALCorpus Christi, TX
Macon County, AL
St. Landry Parish, LA
Madison County, TN
Garfield County, OK
Marianna, AR
McGehee, ARPoinsett County, AR
Somerset, LA
Winnsboro, LA
Mean Median
0
2
4
6
8
10
12
14
16
Flupyradifurone (LC50
-ug/ml)
Variability
LC50
(ug/ml Leaf D
ip)
0
2
4
6
8
10
12
14
16
18
Sulfoxaflor 2014 Sulfoxaflor 2015 Flupyradifurone 2015
Correlating Susceptibility 2015
Key Findings• Developed baseline susceptibility
data for sulfoxaflor and flupyradifurone.
• Sulfoxaflor slightly more toxic to SCA than flupyradifurone.
• Toxicity of sulfoxaflor and flupyradifurone were not correlated.
• Sulfoxaflor susceptibility slightly decreased in 2015 relative to 2014.
Next Steps• Need to determine if shipping aphid
samples affects bioassay results.• Continue monitoring.
Discussion