Laboratory Safety Training

5425 Woodward, Suite 300 Detroit, MI 48202

313.577.1200 www.oehs.wayne.edu

This training is required annually for all Wayne State University faculty, staff, and students working in research and/or teaching laboratories with any chemicals.

You must be registered on the Collaborative Institute Training Initiative (CITI) website and complete a quiz hosted on the CITI site in order to fulfill the requirements for this course.

When you have finished the presentation, click on the link on the last slide that will take you to the quiz on the CITI website.

Topics Covered

Content of the OSHA Lab Standard (29 CFR 1910.1450) and the WSU Chemical Hygiene Plan and Emergency Contingency Plan

OSHA, EPA, MDEQ, and DOT regulations affecting university labs

Safety equipment in the laboratory

Safe handling and storage of chemicals

OSHA Hazard Communication & Global Harmonization System of Classifying & Labeling Chemicals, identifying hazards of chemicals

Accessing Safety Data Sheets (SDSs) for the chemicals in the lab

Personal Protective Equipment (PPE)

Lab responsibilities as a hazardous waste generator

Definitions of hazardous waste

Procedures for collection, labeling, storage and removal of waste

Responding to injuries, spills, fires, and other emergencies

The Chemical Hygiene Plan

Written policy required by MIOSHA to comply with the Laboratory Standard.

Contains important information on WSU lab safety policies and procedures, your rights and responsibilities.

A copy of the plan should be available in your lab.

Download the WSU Chemical Hygiene Plan

Specific Standard Operating Procedures are required in your lab

Specific to experiments and hazards No required format Required content:

hazard controls personal protective equipment health & safety information decontamination, waste disposal, etc.

SOP template is available here

Hazard Communication 9 Hazard Pictograms

Health Hazards (regulated by MIOSHA)

Physical Hazards (regulated by MIOSHA)

Environmental Hazards (Regulated by DEQ)

Chemical Classifications: Health Hazards

Acute Toxicity Skin Corrosion/Irritation Respiratory or Skin Sensitization Germ Cell Mutagenicity Carcinogenicity Reproductive Toxicity Specific Target Organ Toxicity – Single Exposure Specific Target Organ Toxicity – Repeated Exposure Aspiration Simple Asphyxiants

Chemical Classifications: Physical Hazards

Explosives Flammables Aerosols Oxidizers Compressed Gases Self-Reactive Chemicals Pyrophorics Self-heating Chemicals Chemicals, which in contact with water, emit

flammable gases

Labels: Pictograms – Health Hazards

• Acute toxicity (Less Severe): • Irritant • Dermal sensitizer • Acute toxicity (harmful) • Narcotic effects • Respiratory tract irritation

• Acute toxicity (Severe)

Labels: Pictograms – Health Hazards

• Skin corrosion • Serious eye damage/ • Eye irritation

• Carcinogen • Respiratory sensitizer • Reproductive toxicity • Target organ toxicity • Mutagenicity • Aspiration Hazard

Labels: Pictograms – Physical Hazards

• Flammables • Self reactives • Pyrophorics • Self heating • Emits flammable gas • Organic peroxides

• Explosives • Self reactives • Organic peroxides

Labels: Pictograms – Physical Hazards

Corrosive to Metals

Gases under Pressure

Oxidizer

Labels: Signal Word

These are words used to indicate the severity of the hazard and alert employees to the potential hazard. There are two signal words. Only one of these words will appear on the chemical label:

“DANGER”(more severe hazard) “WARNING” (less severe hazard)

Not all labels will have a signal word. Some chemicals are not hazardous enough to require that a signal word appear on the label.

Labels on Chemical Containers All containers must be labeled.

Existing labels on incoming containers must not be removed or defaced.

Make sure that labels stay attached to the container so that the contents are always known.

Labels on Chemical Containers

Secondary or “transfer” containers must be labeled if the chemical will not be used up within one work shift and constantly under the control of the user.

Labels must be legible and prominently displayed on the container.

Safety Data Sheets (SDS)

SDSs provide you with: Chemical and physical properties Health and physical hazards Toxicity information Compatibility/Incompatibility Spill and fire response Personal protective equipment

And much more...

SDSs can be easily accessed online or by contacting OEH&S at 313-577-1200.

SDSs that arrive with a shipment to your lab must be kept on file and made available to staff.

Laboratory Door Signs

Caution signs and the accompanying hazard labels are provided by OEH&S.

They are posted to inform building occupants and emergency responders of the hazards present in lab.

Lab must fill in emergency contact names & numbers!

Label Chemical Storage Areas according to the hazard!

Chemical Fume hoods are certified annually by OEH&S

Line the bottom of the window sash up with the arrow on the side of the hood to ensure maximum protection when performing work.

A sticker on the hood will list the most recent date it was tested, the person who performed the test, if it passed certification, and the airflow in linear feet per minute (lfm).

Set sash at correct height (look for the arrow)

Wear PPE (Personal Protective Equipment)

Work at least 6” into the hood

Keep hood uncluttered.

Chemical Fume Hood Use

Do not use hoods to store chemicals or supplies!

It’s important that air flow in the chemical fume hood is unobstructed.

Large equipment

placed in a fume hood, should be on blocks or racks to allow air flow under the equipment.

Chemical Fume Hood Use

Excess storage and clutter may affect the performance of the hood and increase the risk of spills and accidents.

A biological safety cabinets (BSC) protects the user and the research from contamination. BSCs are not intended for chemical use. They are certified annually by OEH&S.

Know the location of eyewash, safety shower, emergency equipment!

Eyewashes built into the sink should be flushed weekly to ensure proper functioning and clean water.

Post this log near eyewash to keep track:

Safety showers and combined units are tested by OEH&S.

www.oehs.wayne.edu/forms/eyewash_log_sheet.doc

Fire Extinguishers

Know where fire extinguishers are located! should be mounted on wall checked by Office of Risk

Management: 7-3110

Replaced or repaired by FP&M: 7-4315

Wear personal protective equipment (PPE)! The essentials: Lab Coat Appropriate Gloves Appropriate Eye Protection

Glove Selection

select gloves appropriate for the task check gloves for leaks double glove if necessary be alert to unusual sensations in hands do not touch your face, telephone, etc with

contaminated gloves remove gloves before leaving lab

Safety Eyewear

“must be worn in any area where there is the potential for eye injury”

Eye protection used must be ANSI approved (“Z87” stamped on the sidebar)

Must be appropriate for the hazard – goggles, safety glasses with side shields, etc.

If prescription glasses are not ANSI approved, you must wear safety glasses, safety goggles or a full face shield over them

Wear closed-toe shoes and clothing that covers your skin completely.

Remove gloves and other PPE before leaving lab.

NO Eating, drinking, applying cosmetics, etc. is allowed in labs.

No eating/drinking in labs

Food and drink must be stored and consumed in an area outside of the lab, separated by floor to ceiling walls.

Secure cylinders properly… this isn’t the right way!

These cylinders are properly secured

Properly segregate chemicals and put them away when not in use!

Liquid chemicals should be stored by class:

flammables organic acids mineral acids bases oxidizers carcinogens/highly toxic

reagents Where storage space is limited, separation using plastic tubs is acceptable.

Chemical Storage

Hazardous Waste Disposal & Emergency Procedures at WSU

These regulations come from:

U.S. Environmental Protection Agency (EPA) Resource Conservation & Recovery Act (RCRA)

Michigan Department of Environmental Quality (MDEQ)

U.S. Department of Transportation (DOT)

Labs are considered hazardous waste satellite accumulation areas

These areas are allowed to accumulate up to 55 gallons of hazardous waste

or

1 quart of acutely hazardous waste (P-Listed wastes)

For more information on EPA Listed Wastes, click here:

www.epa.gov/osw/hazard/wastetypes/listed.htm

What is a “Hazardous Waste”?

Spent organic solvents Most “wash” solutions Most metals and solutions of metal salts Mixtures of non-hazardous waste with hazardous waste Any waste which meets these EPA characteristics:

FLAMMABLE CORROSIVE REACTIVE TOXIC

Submit all requests for hazardous waste disposal and empty

containers on-line

Chemical Biological

Radioactive

http://www.oehs.wayne.edu

Online form for chemical waste disposal requests: http://www.oehs.wayne.edu/forms/hazardous-waste-pickup.php

Complete every field for each individual container of waste.

Request empty waste containers and tags, and fill in any additional requests or comments at the bottom before submitting a request.

Waste Collection Instructions 3 Golden Rules

Properly tag and label waste as soon as a new container is begun.

Keep waste containers closed when not adding waste to them.

Store liquid waste containers in secondary containers at all times.

Properly tag and label waste containers!

ATTACH A WASTE TAG and record contents when first waste is added to container.

Write clearly in ballpoint pen

Do not separate copies.

Write COMPLETE NAMES of all chemicals, including water (do not write H20).

Estimate the % volume of each component in the container.

Properly tag and label waste containers!

Waste tags must be clear & legible! Replace or write over tags if necessary.

Request Free Waste Containers

Liquid Chemical Waste SHARPS

Find forms at: http://www.oehs.wayne.edu

Solid Chemical Waste

Biological Waste

Store Liquid Waste in Secondary Containers

Use the proper primary containers: 5 gallon plastic carboys

(from OEH&S) Triple-rinsed used

solvent bottles

Store liquid waste containers in secondary plastic containers to protect from leaks/spills.

Always remove funnels and keep containers closed when you are not adding waste!

Solid Chemical Waste Disposal (e.g. ethidium bromide gel waste)

Request pails from the OEH&S website on the Chemical Waste Disposal Request Form.

Keep containers labeled and closed at all times.

Secondary containers are not required for solids.

Waste Glass & Plastic Disposal Only uncontaminated or decontaminated glass &

plastic can go into boxes. Boxes should be sturdy and in good condition.

Uncontaminated Waste Glass & Plastic Disposal

Line box with trash bag. Keep weight below 25 lbs. Tape boxes closed securely

when full Label “waste glass” or

“broken glass”. Custodians dispose of

these in the regular trash.

Needles, razor blades, lancets, etc. are NEVER disposed of in trash bags or cardboard boxes.

ONLY in SHARPS containers!

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In Case of Fire! In the event of a fire, pull

the closest fire alarm pull station.

This rings to the WSU Police Station who then dispatches the Detroit Fire Dept.

Exit the building by the closest stairwell. Do not use the elevator!

Emergency Evacuation Information

Refer to these to familiarize yourself with the closest stairwell to exit in an emergency.

Floor plan diagrams are posted on each floor in your building.

Take steps to prevent chemical spills

Store chemicals on sturdy shelves, away from edge, on shelves w/ lips.

Reduce clutter, eliminate trip hazards/obstructions.

Transport chemicals in carts with sides or in secondary containers.

Chemical Spills and Releases If you have any doubt about whether a spill can be safely

cleaned up by staff in the lab, get help!

Confine the area! Close doors and call from a safe location.

In an emergency or after hours, contact WSU Police at 313-577-2222.

Non-emergency spills during regular office hours, contact OEH&S at 313-577-1200.

Provides prompt & safe clean-up of hazardous materials spills, 24 hrs/day- 365 days/year.

Call OEH&S 313-577-1200 for more information.

WSU OEH&S Hazmat Response Team

Minor Chemical Spills Minor spills… do not spread rapidly do not endanger people or

property except by direct contact

do not endanger environment

Consider: amount of material spilled hazards of chemical(s) involved if you have proper equipment to

clean up the spill Use common sense and call for help if you aren’t sure!

Cleaning up a minor spill

Wear lab coat, gloves, eye protection

Prevent spread of liquid / vapors

Absorb with spill pads, towels, etc.

Neutralize acids and bases

Clean area with soap & water

Decontaminate area

Label contaminated materials and notify OEH&S for dispos

Mercury Spills ALWAYS report mercury spills to OEH&S!

Mercury is a toxic material!

A spill can split into small beads that are difficult to see.

The beads can spread far and hide in cracks and crevices.

Mercury vaporizes at room temperature.

Mercury vapors are invisible and have no odor.

Chronic exposure can be a health hazard.

Mercury Spills ALWAYS report mercury spills to OEH&S!

Do not attempt to clean up a mercury spill on your own.

Isolate / block off the area surrounding the spill to prevent the contamination from spreading.

Call OEH&S for assistance at 313-577-1200.

After hours, contact the WSU Police at 313-577-2222.

Dangerous Goods Shipping: Regulatory Overview (awareness)

By law, anyone who packs, ships, transports or receives dangerous goods must be properly trained.

Who’s the law? US Department of Transportation (DOT)

Training must be renewed every 2 years.

These slides do NOT count as your initial training!

The shipper bears legal responsibility for shipments.

Penalties for non-compliance can result in the big fines:

Up to $250,000 and up to a year jail sentence for individuals

Up to $500,000 per incident for organizations

There are nine classes of Dangerous Goods or Hazardous Materials

Class 1: Explosives Class 2: Gases Class 3: Flammable

Liquids Class 4: Flammable

Solids Class 5: Oxidizers and

Organic Peroxides

Class 6: Toxic (poisonous) and Infectious Substances 6.1 Toxic Substances 6.2 Infectious Substances

Class 7: Radioactive Class 8: Corrosive Class 9: Miscellaneous (dry ice)

Class 6 and Class 9 are most commonly shipped by labs.

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Shipping Dangerous Goods

Common materials shipped include: human and animal samples, bacteria, DNA, proteins, chemicals (solids or liquids), dry ice, etc.

Shipping ANY material on dry ice by air is considered a Dangerous Good/Hazardous Material

OEH&S is the designated authority for shipping Dangerous Goods from WSU.

Contact OEH&S at 577-1200 to ship any Dangerous Goods.

Additional Information WSU Chemical Hygiene Plan http://www.oehs.wayne.edu/environmental/chemical-hygiene-plan.pdf

WSU Hazardous Materials Management http://www.oehs.wayne.edu/hazardous/index.php

WSU Emergency Procedures http://www.oehs.wayne.edu/procedures/emergency.php

“Experimenting With Danger” Lab Safety Video: http://www.youtube.com/watch?v=ALBWxGik64A&feature=share&list=PL8690AD327F838FC0&index=4

Office of Environmental Health & Safety

5425 Woodward, Suite 300 Detroit, MI 48202

313.577.1200 http://www.oehs.wayne.edu

Take the Quiz! To verify your completion of this course through WSU, you must return to the CITI site and take the quiz.

If you are unable to register on CITI, you may download the quiz here. Questions? Call 577-1200 or e-mail: fracassa@wayne.edu