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Laboratory Safety and Pro Laboratory Safety and Pro cedures cedures Laboratory Safety and Laboratory Safety and Procedures Procedures Safety Rules 1

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Laboratory Safety and ProcedureLaboratory Safety and Proceduress

Laboratory Safety and Laboratory Safety and Procedures Procedures

Safety Rules 1

Laboratory Safety and ProcedureLaboratory Safety and Proceduress

Laboratory Safety and Laboratory Safety and Procedures Procedures

Why Worry About Why Worry About Safety?Safety?

Laboratory Safety and ProcedureLaboratory Safety and Proceduress

1. Be prepared to work when you 1. Be prepared to work when you arrive at the laboratory. Familiarize arrive at the laboratory. Familiarize yourself with the lab procedures yourself with the lab procedures before beginning the lab.before beginning the lab.

2. Perform 2. Perform onlyonly those lab activities those lab activities assigned by your teacher. assigned by your teacher. Never do anything in the laboratory Never do anything in the laboratory

that is not called for in the laboratory that is not called for in the laboratory procedure or by your teacher. procedure or by your teacher.

Never work alone in the lab. Never work alone in the lab. Do not engage in any horseplay.Do not engage in any horseplay.

Laboratory Safety and ProcedureLaboratory Safety and Proceduress

3. Work areas should be kept clean 3. Work areas should be kept clean and tidy at all times. and tidy at all times. Only lab manuals and notebooks should Only lab manuals and notebooks should

be brought to the work area.be brought to the work area. Other books, purses, brief cases, etc.. Other books, purses, brief cases, etc..

should be left at your desk should be left at your desk 4. Clothing should be appropriate for 4. Clothing should be appropriate for

working in the lab. Jackets, ties, and working in the lab. Jackets, ties, and other loose garments should be other loose garments should be removed. removed. Open-toed shoes should Open-toed shoes should not be worn.not be worn.

Laboratory Safety and ProcedureLaboratory Safety and Proceduress

5. Long hair should be tied back 5. Long hair should be tied back or covered, especially in the or covered, especially in the vicinity of open flame.vicinity of open flame.

6. Jewelry that might present a 6. Jewelry that might present a safety hazard, such as dangling safety hazard, such as dangling necklaces, chains, medallions, or necklaces, chains, medallions, or bracelets should not be worn in bracelets should not be worn in the lab.the lab.

7. Follow all instructions, both 7. Follow all instructions, both written and oral, carefully.written and oral, carefully.

Laboratory Safety and ProcedureLaboratory Safety and Proceduress

8. Safety goggles and lab aprons should 8. Safety goggles and lab aprons should be worn at be worn at all timesall times..

9. Set up apparatus as described in the 9. Set up apparatus as described in the lab manual or by your teacher. Never lab manual or by your teacher. Never use makeshift equipment.use makeshift equipment.

Laboratory Safety and ProcedureLaboratory Safety and Proceduress

10. Always use the proper instrument 10. Always use the proper instrument (tongs, test tube holder, forceps, etc.) (tongs, test tube holder, forceps, etc.) for handling apparatus or equipment.for handling apparatus or equipment.

11. Keep all combustible materials 11. Keep all combustible materials away from open flames. away from open flames.

12. Never touch any substance in the 12. Never touch any substance in the lab unless specifically instructed to do lab unless specifically instructed to do so by your teacher.so by your teacher.

13. Never put your face near the mouth 13. Never put your face near the mouth of a container that is holding chemicals.of a container that is holding chemicals.

Laboratory Safety and ProcedureLaboratory Safety and Proceduress

14. Never smell any chemicals unless 14. Never smell any chemicals unless instructed to do so by your teacher. instructed to do so by your teacher. When testing for odors, use a wafting When testing for odors, use a wafting motion to direct the odors to your motion to direct the odors to your nose. nose.

15. Any activity involving poisonous 15. Any activity involving poisonous vapors should be conducted in the vapors should be conducted in the fume hoodfume hood..

16. Dispose of waste materials as 16. Dispose of waste materials as instructed by your teacher.instructed by your teacher.

17. Clean up all spills immediately.17. Clean up all spills immediately.

Laboratory Safety and ProcedureLaboratory Safety and Proceduress

18. Clean and wipe dry all work 18. Clean and wipe dry all work surfaces at the end of class.surfaces at the end of class. Wash Wash your hands thoroughly. your hands thoroughly.

19. Know the location of 19. Know the location of emergency equipment (First aid emergency equipment (First aid kit, fire extinguisher, safety kit, fire extinguisher, safety shower, fire blanket, etc.) and how shower, fire blanket, etc.) and how to use them.to use them.

20. Report 20. Report allall accidents to the accidents to the teacher immediately, regardless of teacher immediately, regardless of how small it seems to be.how small it seems to be.

Laboratory Safety and ProcedureLaboratory Safety and Proceduress

21. Read and double check labels on 21. Read and double check labels on reagent bottles before removing any reagent bottles before removing any reagent. Take only as much reagent as reagent. Take only as much reagent as you need.you need.

22. Do 22. Do notnot return unused reagent to return unused reagent to stock bottles- share with other students.stock bottles- share with other students.

23. When transferring chemical reagents 23. When transferring chemical reagents from one container to another, hold the from one container to another, hold the containers out away from your body.containers out away from your body.

24. When mixing an acid and water, 24. When mixing an acid and water, always add the acid to the water.always add the acid to the water.

Laboratory Safety and ProcedureLaboratory Safety and Proceduress

25. Avoid touching chemicals with your 25. Avoid touching chemicals with your hands. If chemicals do come in contact hands. If chemicals do come in contact with your hands, wash them with your hands, wash them immediately.immediately.

26. Notify your teacher if you have any 26. Notify your teacher if you have any medical problems that might relate to medical problems that might relate to lab work, such as allergies or asthma.lab work, such as allergies or asthma.

27. If you will be working with chemicals 27. If you will be working with chemicals in the lab, avoid wearing contact lenses. in the lab, avoid wearing contact lenses. Change to glasses, if possible, or notify Change to glasses, if possible, or notify the teacher- there are special goggles the teacher- there are special goggles (nonvented) for you to wear.(nonvented) for you to wear.

Laboratory Safety and ProcedureLaboratory Safety and Proceduress

28. Glass tubing, especially long pieces, 28. Glass tubing, especially long pieces, should be carried in a vertical position should be carried in a vertical position to minimize the likelihood of breakage to minimize the likelihood of breakage and to avoid stabbing anyone.and to avoid stabbing anyone.

29. Never handle broken glass with your 29. Never handle broken glass with your bare hands. Use a brush and dustpan to bare hands. Use a brush and dustpan to clean up broken glass. Dispose of the clean up broken glass. Dispose of the glass in the “Broken Glass Box”, or as glass in the “Broken Glass Box”, or as directed by your teacher.directed by your teacher.

Laboratory Safety and ProcedureLaboratory Safety and Proceduress

30. Always lubricate glassware 30. Always lubricate glassware (tubing, thistle tubes, thermometers, (tubing, thistle tubes, thermometers, etc.) with water or glycerin before etc.) with water or glycerin before attempting to insert it into a rubber attempting to insert it into a rubber stopper.stopper.

31. Never apply force when inserting 31. Never apply force when inserting or removing glassware from a or removing glassware from a stopper. Use a twisting motion. If a stopper. Use a twisting motion. If a piece of glassware becomes "frozen" piece of glassware becomes "frozen" in a stopper, take it to your teacher.in a stopper, take it to your teacher.

Laboratory Safety and ProcedureLaboratory Safety and Proceduress

32. Do not place hot glassware 32. Do not place hot glassware directly on the lab table. Always directly on the lab table. Always use an insulating pad of some use an insulating pad of some sort, such as the base of your ring sort, such as the base of your ring stand.stand.

33. Allow plenty of time for hot 33. Allow plenty of time for hot glass to cool before touching it. glass to cool before touching it. Hot glass can cause painful burns. Hot glass can cause painful burns. (Remember: (Remember: HotHot glass looks cool.) glass looks cool.)

Laboratory Safety and ProcedureLaboratory Safety and Proceduress

34. Exercise extreme caution when 34. Exercise extreme caution when using a gas burner. Keep your head using a gas burner. Keep your head and clothing away from the flame.and clothing away from the flame.

35. Always turn the burner off 35. Always turn the burner off when it is not in use.when it is not in use.

36. Do not bring any substance 36. Do not bring any substance into contact with a flame unless into contact with a flame unless instructed to do so.instructed to do so.

37. Never heat anything without 37. Never heat anything without being instructed to do so.being instructed to do so.

Laboratory Safety and ProcedureLaboratory Safety and Proceduress

38. Never look into a container 38. Never look into a container that is being heated.that is being heated.

39. When heating a substance in 39. When heating a substance in a test tube, make sure that the a test tube, make sure that the mouth of the tube is not pointed mouth of the tube is not pointed at yourself or anyone else.at yourself or anyone else.

40. Never leave unattended 40. Never leave unattended anything that is being heated or is anything that is being heated or is visibly reacting.visibly reacting.

Laboratory Safety and ProcedureLaboratory Safety and Proceduress

Lab Safety:Lab Safety:Everyone is Responsible!Everyone is Responsible!

Safety glassesSafety glasses Contact lensesContact lenses Long hair and bulky clothingLong hair and bulky clothing Rings, watches, and dangling jewelryRings, watches, and dangling jewelry

Laboratory Safety and ProcedureLaboratory Safety and Proceduress

Accidents?Accidents?

Accidents Accidents cancan happen happen Remain calmRemain calm Report Report ALLALL accidents immediately accidents immediately Prevention!Prevention!

watch & help your partnerwatch & help your partner

Laboratory Safety and ProcedureLaboratory Safety and Proceduress

Types of Accidents &Types of Accidents &How to Handle themHow to Handle them

A. Broken GlassA. Broken Glass most commonmost common stop where you are; assess situationstop where you are; assess situation move when safe; report cutsmove when safe; report cuts call instructor over call instructor over chemicals involved?chemicals involved?

Laboratory Safety and ProcedureLaboratory Safety and Proceduress

Types of Accidents &Types of Accidents &How to Handle themHow to Handle them

B. Scrapes and CutsB. Scrapes and Cuts report immediately to teacherreport immediately to teacher possibility of infectionpossibility of infection do not contact another’s blooddo not contact another’s blood

what if blood is present?what if blood is present?

Laboratory Safety and ProcedureLaboratory Safety and Proceduress

Types of Accidents &Types of Accidents &How to Handle themHow to Handle them

C. Chemical SpillsC. Chemical Spills chemical safety warning signschemical safety warning signs small mess or major disaster?small mess or major disaster? treat all as dangerous; respecttreat all as dangerous; respect wait for instructor’s directionswait for instructor’s directions on your skin or clothing?on your skin or clothing?

Laboratory Safety and ProcedureLaboratory Safety and Proceduress

Types of Accidents &Types of Accidents &How to Handle themHow to Handle them

D. FireD. Fire lab burnerslab burners

when not in use, turn it offwhen not in use, turn it off

paperpaper place in sink; run waterplace in sink; run water

clothing or hairclothing or hair fire blanket; fire extinguisherfire blanket; fire extinguisher

Laboratory Safety and ProcedureLaboratory Safety and Proceduress

Lab Safety EquipmentLab Safety Equipment

eye wash stationeye wash station safety showersafety shower fire safety blanketfire safety blanket fire extinguisherfire extinguisher first aid kitfirst aid kit fume hoodfume hood

•spill kit

•goggle cabinet

Laboratory Safety and ProcedureLaboratory Safety and Proceduress

Clean-Up:Clean-Up:

follow instructor’s directionsfollow instructor’s directions NEVER put back in bottleNEVER put back in bottle return bottles to proper placereturn bottles to proper place wash/dry glassware; store itwash/dry glassware; store it clean/dry your lab areaclean/dry your lab area

Laboratory Safety and ProcedureLaboratory Safety and Proceduress

Additional Resources from Additional Resources from the Internet:the Internet:

Laboratory Survival ManualLaboratory Survival Manual

How to Practice Safe ScienceHow to Practice Safe Science

Material Safety Data SheetsMaterial Safety Data Sheets