WATER - JACKETED CO2

INCUBATORS

MODELS: 39200-00, 39200-05

INSTALLATION AND OPERATION MANUAL

Cole-Parmer Instruments_____________________________________________________________________ 625 East Bunker Court ¥ Vernon Hills, IL 60061 ¥ Phone (847) 549-7600 ¥ Fax (847) 549-7676

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TABLE OF CONTENTS

SECTION 1.0 RECEIVING AND INSPECTION

SECTION 2.0 INSTALLATION

SECTION 3.0 CONTROL PANEL OVERVIEW

SECTION 4.0 OPERATION

SECTION 5.0 FYRITE READING

SECTION 6.0 MAINTENANCE

SECTION 7.0 TROUBLESHOOTING

SECTION 8.0 PARTS LIST

UNIT SPECIFICATIONS

SCHEMATICS

REV 6/99#4861323

These units are CO2 Water Jacketed incubators for professional, industrial oreducational use where the preparation or testing of materials is done atapproximately atmospheric pressure and no flammable, volatile or combustiblematerials are being heated. These units are not intended for hazardous or householdlocations or use.

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RECEIVING AND INSPECTIONIMPORTANT: READ THIS MANUAL IMMEDIATELY

Your satisfaction and safety require a complete understanding of this unit,including its proper function and operational characteristics. Be sure operatorsare given adequate training before attempting to put the unit in service. Note: Thisequipment must be used only for its intended application; any alterations ormodifications will void your warranty.

1.1 Inspection: The carrier, when accepting shipment, also accepts responsibilityfor safe delivery and is liable for loss or damage claims. On delivery, inspectfor visible exterior damage, note and describe on the freight bill any damagefound, and enter your claim on the form supplied by the carrier.

1.2 Inspect for concealed loss or damage on the unit itself, both interior andexterior. If any, the carrier will arrange for official inspection to substantiate yourclaim.

1.3 Return Shipment: Save the shipping carton until you are sure all is well. If forany reason you must return the unit, contact your customer servicerepresentative for authorization. Supply nameplate data, including modelnumber and serial number.

1.4 Accessories: Make sure that all of the equipment indicated on the packing slipis included with the unit. Carefully check all packaging before discarding. Eachunit comes equipped with 6 shelves, 2 shelf stands, 1 humidity pan, 4 levelingfeet, supply hose kit and a power cord.

Section

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4

INSTALLATIONLocal city, county, or other ordinances may govern the use of this equipment. If youhave any questions about local requirements, please contact the appropriate localagency. Installation may be performed by the end user.

Under normal circumstances this unit is intended for use indoors, at roomtemperatures between 5° and 40°C, at no greater than 80% Relative Humidity (at 25°)and with a supply voltage that does not vary by more than 10%. Customer serviceshould be contacted for operating conditions outside of these limits.

CAUTION: Make sure that the incubator is located in its intended position and levelbefore filling the water jacket.

2.1 Power Source: The power supply must be properly grounded (earthed) andcorrectly sized to match the unit nameplate rating. The supply voltage must bematch the nameplate voltage within +/- 10%. These units are intended for50/60 HZ application. If supplied with a detachable cord set, plug the femaleend into the inlet on the unit and the male plug into the supply. Assure thatunits requiring a fuse have a fuse installed. This fuse may be at the inlet or apart of the cord set male plug.

2.2 Location: Select a site for the incubator with consideration for conditions whichmay effect performance, such as heat from radiators, ovens, autoclaves, etc. Avoid direct sun, fast moving air currents, heating/cooling ducts, and high-trafficareas. To ensure air circulation around the unit, allow a minimum of 5cmbetween the incubator and any walls or partitions which might obstruct free airflow.

2.3 Lifting / Handling: These units are heavy and care should be taken to useappropriate lifting devices that are sufficiently rated for these loads. Unitsshould only be lifted from their bottom surfaces. Doors, handles and knobs arenot adequate for lifting or stabilization. The unit should be completelyrestrained from tipping during lifting and transport. All moving parts, such asshelves and trays should be removed and doors need to be positively locked inthe closed position during transfer to prevent shifting and damage.

Section

2

5

2.4 Leveling: The unit must sit level and solidly. Leveling feet are supplied, and areto be installed in the holes in the base of the unit. Turn them counterclockwiseto raise level. If the unit must be moved, turn the leveling feet in all the wayclockwise to prevent damage.

2.5 Cleaning: The unit chamber should be cleaned and disinfected prior to use. Your operating conditions and appropriate protocol will determine the correctprocedure for decontamination. A typical decontamination procedure that isadequate for many situations has been described below. As well, certainsteps are listed that will help reduce the likelihood of contamination and thenecessity of decontamination. Whatever process is appropriate, it needs to bedone on a regularly scheduled basis. Depending on usage and protocol, thismay be monthly, quarterly or otherwise. Regardless of the decontaminationprocedure used, certain precautions will need to be taken:

A. Always disconnect the unit from the electrical service when cleaning.Assure all volatile or flammable cleaners are evaporated and dry beforereconnecting the unit to the power supply.

B. Special care should be taken when cleaning around sensing heads toprevent damage.

C. If you use chlorine-based bleaches or abrasive cleaners this will modifythe stainless steel interior finish. DO NOT USE hard tools such asmetal wire brushes or steel wool. Use non-abrasive cleaners and softtools such as plastic brushes.

D. Use only DISTILLED water in the water jacket and the humidity pan. DONOT USE de-ionized water due to its reactivity with metal components.

TYPICAL DECONTAMINATION PROCEDURE

1. Remove the humidity pan every week and autoclave, or wash with soapand water then disinfect with 70% alcohol solution. Replace in theincubator with fresh, DISTILLED water.

2. Flush the sample port tubing with 70% alcohol solution. Replace anylines that have a green color.

3. Remove the door gaskets, clean and disinfect. Clean and disinfect allmounting grooves for the door gaskets.

4. Remove all shelves, shelf supports, shelf standards and shields. Autoclave, or wash and disinfect as described in item 1.

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5. Wash and disinfect all interior surfaces.

6. Give special attention to cleaning and disinfecting all access ports, airbleeds, shaft holes, electrical pass-throughs and any other passagesinto the chamber.

7. Replace all air and CO2 filters every six months or when noticeably dirtyon the upstream side. CO2 filters are located in the shadow box justbehind the GAS IN fitting and in line with the CO2 tubing kit. Units withflow meters require operator- supplied air pumps. These pumps shouldbe checked for proper air filtering.

OPERATION FOR MINIMIZING CONTAMINATION

1. Keep the outside of the incubator, including the air in the laboratory, asclean as possible. This is particularly important for units placed directlyon the floor. Do not place incubators near doors, air vents or otherareas of high air movement or traffic.

2. The floor around the unit needs to be clean. Units that are placed on thefloor should be mounted higher Ð typically on a caster platform Ð forease of moving the unit during cleaning and access to the back of theunit.

3. Minimize the number of times access is made to the chamber duringnormal operation.

4. Do not depend on the use of antibiotics to maintain uncontaminatedconditions, as this is an inadequate technique for sterilization. Preferably use aseptic techniques as described above for maintainingsterile conditions in the incubator.

2.6 Filling and Draining: Turn the power switch to the I/On position. The frontpanel has a WATER JACKET LOW warning light. Fill the water jacket withdistilled water only through the filling port, which is labeled "Fill W/J Here." Stopfilling when the warning light goes off. Each chamber should containapproximately 19 gallons; DO NOT over-fill the water jacket. To drain the waterjacket, attach siphon hose to siphon tube adjacent to fill port and drain.

2.7 Shelves and Interior Parts: Shelving, shelf supports and humidity pan aresupplied with each unit. See Figure 1 for proper placement of theseaccessories.

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Figure 1

9850754

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CONTROLS OVERVIEW (See Figure Two)

3.1 Power Switch: The main power I/O (On/Off) switch on the panel controls allpower to the incubator. It must be in the I/On position before any systems areoperational. The POWER ON light indicates power to the unit.

3.2 Main Temperature Controller: Marked C°, this controller contains the digitaltemperature display, UP and DOWN arrow pads, HIGH and LOW alarmindicators and an alarm MUTE indicator.

A. Digital Temperature Display indicates the actual temperature within theincubator chamber to .1°C.

B. UP and DOWN Arrow Pads are used for inputting the set point,calibrating the display and muting or unmuting the audible alarm.

C. HIGH and LOW Alarm Indicator will light whenever there is an alarmcondition associated with the temperature within the incubator.

D. Alarm MUTE Indicator will light whenever the audible alarm has beenmuted.

3.3 Element Energized: This pilot lamp is on whenever the temperature controlleris energizing the heating element. The element will go on and off when heat isneeded to maintain the set point temperature.

3.4 High Limit Thermostat: Completely independent of the Main TemperatureController, the High Limit Thermostat functions as an override control. If at anytime the Main Temperature Control fails in the On position, the chambertemperature is then limited to the High Limit Thermostat set point which isapproximately (but not limited to) 1°C above the Main Controllers set point. Note that the ELEMENT ENERGIZED indicator will continue to function underthe control of the High Limit Thermostat.

Section

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3.5 Temperature High Limit: This pilot lamp is on whenever the High LimitThermostat has taken control of the incubator and shut down the elements. Under normal operating conditions this pilot lamp should never be on.

NOTE: If the unit is being run on the High Limit Thermostat, the temperaturestability of the unit will suffer.

3.6 CO2 Sample Port: Chamber atmosphere can be taken from the Sample Porton the control panel. CO2 concentration can then be checked using a Fyrite gasanalyzer or other instrument such as a gas chromatograph.

3.7 Door Temperature Controller: Located inside the outer door. The DoorTemperature Controller maintains temperature level of the door elements tominimize condensation on the inner glass door. It provides fast heat-up andtemperature recovery after the door has been opened, and minimizes chanceof contamination.

3.8 Door Element Energized: This pilot lamp is on whenever the door temperaturecontroller is energizing the heating element. The element will go on and off asheat is needed to maintain the door temperature set point.

3.9 Water Jacket Low: This pilot lamp is on whenever the water level in the jacketis low indicating distilled water should be added.

3.10 Purge & Purge Timer: The solid state timer and Purge button is used to injectCO2 into the chamber to bring the CO2 concentration up to the desired level.

3.11 CO2 Flow Meter: The valve at the base of the meter is used to regulate flow ofCO2 to the incubator interior. WARNING: Don't use valve at base of meter forCO2 shut-off when unit is out of service. Shut CO2 off at tank.

3.12 Air Flow Meter: The valve at base of meter is used to regulate flow of air toincubator.

Figure 2

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OPERATIONREAD THIS SECTION IN ITS ENTIRETY BEFORE ATTEMPTINGOPERATION!

Important: In order to ensure the proper operation of this incubator, it is essentialthat you regularly verify the accuracy of the temperature and CO2 controls. Temperature can be confirmed by use of a Fyrite Gas Analyzer or other equipmentsuch as gas chromatograph. These must be checked at least on a weekly basis. Any questions should be directed to a technical service representative.

4.1 Power Source: Connect service cord to grounded outlet; attach female end ofthe cordset into the fused and recessed male plug at the back of the unit andthe male plug end of the cordset into the building supply. NOTE: Fill waterjacket (See 2.6) and set temperature before connecting CO2 supply into theincubator.

4.2 Humidity: Humidification of the incubator is achieved by the evaporation ofwater from the humidity pan in the bottom of the incubator. Fill the stainlesssteel pan supplied with DISTILLED WATER ONLY and place it on the floor ofthe chamber to achieve near saturation humidity. For a quicker recovery ofhumidity, as well as higher humidity levels, use a pan with the largest possiblesurface area. Stainless steel is the best choice for a humidity pan, do not useplastic, glass, or any other metal. Do not use corrosive chemicals such ascopper sulfate or chlorine. Such chemicals should never be placed directly inbottom of chamber. The use of disinfecting chemicals within the humidity panis not recommended because they can change the humidity by changingsurface tension of the water and inhibiting cell culture growth. Water in the panshould be changed at least once a week to help control contamination and tomaintain proper evaporation. Do not use tap water or deionized water to refillthe humidity pan. Use Distilled Water Only for this purpose.

4.3 Turn the High Limit Thermostat to its maximum position, clockwise.

4.4 Setting Main Temperature Controller: An accurate reference thermometershould be used inside the chamber when setting and calibrating the incubatortemperature. Be certain that the thermometer can be easily viewed through theglass door and is not touching any shelves or chamber walls as this will give

Section

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an inaccurate reading. To set temperature on the control press either the UP orDOWN arrow pad until the digital display begins to blink from bright to dim. While blinking the display is showing the current set point which can bechanged by pushing the UP and DOWN arrow pads until the desired value isreached. If no adjustments are made within five (5) seconds, the controller willdefault back to displaying the temperature within the chamber. Once set pointis established allow several hours for the temperature to stabilize.

4.5 Calibrating Main Temperature Controller: During Main TemperatureCalibration it is important that the inner glass door not be opened for anyreason, the outer heated door be opened as little as possible and thetemperature has been stabile at set point for several hours. Compare thereference thermometer with the digital display. If there is an unacceptabledifference put the display into calibrate mode by pressing both the UP andDOWN arrow pads at the same time until the display begins to flash. Whileflashing the display can be changed to match the reference thermometer bypushing the UP or DOWN arrow pads. If no adjustments are made within five(5) seconds the display will default back to displaying the temperature in thechamber. After the display has restabilized and maintained set point forseveral hours, check the actual temperature again. If the referencethermometer does not match the display, repeat the calibration.

4.6 Setting Door Temperature Controller: The Door Temperature Control shouldinitially be set at the 38°C guide mark and adjusted upward if necessary toeliminate condensation on the glass door. Note that the door temperature canbe adjusted to keep condensation from forming, but cannot eliminate it onceformed. Use a dry towel to wipe off the inner door before resetting the control. The door temperature should be 1°C to 1.5°C above the Main Temperature. You may wish to use a reference thermometer taped to the center of the doorliner to aid in determining the temperature; if so, the thermometer must be readvery quickly before it drops due to the cooler room temperature. Adjust thecontrol as necessary and wait several hours before checking again. Do not setthe door temperature too high or incubator uniformity will suffer andcondensation will appear on the chamber walls.

4.7 Setting High Limit Thermostat: With the Main control calibrated and stabile atset point, the High Limit needs to be adjusted. Previously turned to itsmaximum position as stated in section 4.3, now turn the control knobcounterclockwise just until the TEMPERATURE HIGH LIMIT indicator lightcomes on. Next, turn the control knob clockwise just until the indicator lightgoes off. Then turn the control knob clockwise two (2) of the smallest divisionson its scale past the point where the indicator light went off. This will adjust theHigh Limit set point to approximately 1°C above the Main Temperature Controlset point. It is recommended that the High Limit set point be checkedperiodically to assure no changes have occurred. A mark on the dial will aid for

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future reference if changes to this set point occur.

4.8 Temperature Alarms: The Main Temperature Control has visual/audible alarmindicators for HIGH and LOW conditions that are activated whenever the actualtemperature is 1°C above or below the set point. There is a built in delay offifteen (15) minutes on the occurrence of the audible LOW alarm. This preventsthe alarm from activating every time the door is opened and the temperaturedrops.

4.9 Muting Audible Alarm: The audible alarms can be muted for a single alarmoccurrence by pressing and holding down either the UP or DOWN arrow padfor several seconds until the alarm is muted and the MUTE indicator comes on. This means the alarm is muted for that particular condition while it exists, butis not muted indefinitely or for a separate condition that may occur.

4.10 CO2 Supply: As stated in section 4.1, do not connect the CO2 supply to theincubator until the Temperatures have been set. Use only medical grade CO2

with an in-line filter from the tank to the incubator. These incubators use CO2 insmall quantities. Precise metering of input is vital for maximum performanceand only a two-stage CO2 pressure regulator is recommended. Note thatsome single-stage CO2 pressure regulators have two (2) gauges, be certainyou are using only a two-stage regulator.

4.11 CO2 Regulator: The high-pressure stage of the regulator coming direct fromthe supply tank must have a range from 0 to at least 2000 PSI (actual tankpressure as read on the gauge). The low-pressure stage should have a rangefrom 0 to at least 40 PSI. The low-pressure gauge indicates actual CO2

pressure into the incubator. Connect the CO2 supply from the low-pressurestage of the regulator to the 3/8" inlet fitting at the back of the unit above the cordinlet.

CAUTION: CO2 pressure to the incubator inlet is rated from 5 to 40 PSI. DONOT exceed 40 PSI.

4.12 Air Supply: The air inlet supply hose fitting is located on the rear of the unit justabove the CO2 TO CHAMBER hose fitting and is marked AIR TO CHAMBER. Supply air to the chamber by means of a lightwear industrial pump with anoutput rating of 2 to 5 liters Per Minute (LPM). In house air systems can beused but make sure a regulator and filter are included in the supply to theincubator. In house air supplies must be regulated to no more than 10 PSI atthe inlet.

4.13 Setting CO2 and Air Flow Rates: Adjust the LPM air flow valve so that the steel

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ball in the flow meter floats at "2". Set the CO2 flow valve according to thedesired percentage. The chart below is a useful guide; however, the settingsare not absolute. Measure the CO2 percentage, after allowing at least 2 hours,then increase or decrease CO2 flow until achieving relative percentages.

Percentage CO2 Setting

5% 100cc7.5% 150cc10% 200cc

Measure the CO2 percentage using a Fyrite or other method, after allowing atleast 2 hours to stabilize. Increase or decrease the air flow meter setting untilthe desired CO2 value is achieved. Make sure the chamber is not tightlysealed. Since air and CO2 are being supplied to the chamber under pressure,a tightly sealed chamber will build internal pressure and cause flow meterrestrictions and gas blow-by around the door gasket. Use stopper type plug inthe chamber access port on the right side of the unit with at least a _ inch holethrough it.

Note: Unless the access port on the right side of the unit is needed for powercords of accessory equipment, leave the stopper in to prevent unnecessary CO2

leakage.

4.14 Setting Purge Timer: The following procedure is explained with theassumption that 5% is the desired CO2 percentage:

A. Set dial to 15 seconds.

B. Open incubator 30 seconds, emptying CO2 content. Close door.

C. Push and release PURGE button, which will inject CO2 into chamberover a 15 second interval. This re-establishes desired CO2/air ratio.

D. Measure CO2 percentage in interior of unit with Fyrite. See section 5.0,FYRITE READING.

E. If CO2 level of interior of incubator is above or below 5%, adjust timer upor down 1 or 2 increments. Allow at least 5 minutes for stabilizationbefore repeating the same procedure and measurement. If you desire ahigher CO2 level, proportionally increase the setting on the Purge Timer.

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FYRITE READINGNOTE: Be sure to hold canister away from face. Do not press button with canisterinverted.

1. Press button on top of Fyrite canister to release CO2 concentration. Tipcanister to the side to ensure all fluid is released from top of canister.

2. Loosen screw on slide scale and align top of fluid with zero on the scale. Tighten screw.

3. Connect hose and bulb to unit being tested.

4. Place hose directly over button on top of canister and press firmly.

5. With button depressed, squeeze bulb 27 times. On the last squeeze,and with bulb still deflated, release hose from button.

6. Turn Fyrite canister upside down 3 times, each time allowing all fluid toflow to the opposite end of the canister.

7. Tip canister slightly to ensure all fluid has been released from top ofcanister.

8. Read CO2 concentration in %.

NOTE: Your Fyrite indicator will come with a complete set of detailedinstructions which should be followed carefully. The fluid used inside thisFyrite instrument is poisonous and corrosive and must not be takeninternally. In the event of a spill or accidental body contact with the Fyritefluid, follow instructions on the refill bottle carefully.

Section

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MAINTENANCE

NOTE: Prior to any maintenance or service on this unit, disconnect service cordfrom the power supply.

6.1 Cleaning: Disinfect the incubator interior and remove and clean shelves andshelf slides on a regular basis. Please review the cleaning procedures in theInstallation section for detailed instructions.

6.2 Clean humidity pan and replenish water as necessary. Fill the stainless steelhumidity pan with distilled water and place it on the floor of the chamber. Fillthe water jacket when the indicator light comes on. DO NOT over fill.

6.3 Periodically check CO2 supply lines and connections for leaks. Use a liquidsoap solution and wet supply line and connections to detect leaks. Look forbubbles.

6.4 Keep the CO2 flow system free from impurities. Erratic CO2 control is usuallytraceable to the CO2 pressure regulator on the tank, impurities in the tank, orimpurities in the solenoid valve. Replace the CO2 in-line filters every six (6)months or when the filter has become noticeably dirty on the upstream side.There is a filter attached to the tubing kit and one inside the shadow boxconnected to the GAS-IN line infront of the solenoid.

6.5 Storage: If the incubator is turned off for any length of time, be it hours, days, orweeks, it may be reactivated by simply turning it on and allowing 24 hours forrecovery. NO adjustment or recalibration is necessary. If it is shut down, openthe door, remove the humidity pan, and disconnect the gas supply. If this is notdone, all the warm water will condense out on the interior of the incubator. Since the water will contain carbonic acid from the CO2, damage may occur. Ifincubator is shut down for storage or transporting, siphon water from jacket,remove humidity pan, remove shelves and shelf standards, turn off CO2 supplyat tank, screw in leveling legs and disconnect the power supply.

Section

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TROUBLESHOOTING

TEMPERATURE

Temp-1 Temperature too

high

1/ controller set too high-see section 4.42/ controller failed on Ð call Customer Service3/ wiring error Ð call Customer Service

Temp-2 Display reads "HI" or

"400"+

probe is unplugged, is broken or wire to sensor isbroken Ð trace wire from display to probe; movewire and watch display to see intermittentproblems

Temp-3 Chamber tempspikes over set pointand then settles toset point

recalibrate Ð see section 4.5Temp-4 Temperature too low

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1/ High Limit too low Ð see section 4.72/ controller set too low Ð see section 4.43/ unit not recovered from door opening Ð wait fordisplay to stop changing4/ unit not recovered from power failure or beingturned off Ð incubators will need 24 hours to warmup and stabilize5/ element failure Ð see if element light is on;compare current draw to data plate6/ controller failure Ð confirm with front panel lightsthat controller is calling for heat7/ High Limit failure Ð confirm with front panel lightsthat it is operating correctly8/ wiring problem Ð check all functions and comparewiring to diagram in section 8.0, especially aroundany areas recently worked on9/ loose connection Ð check shadow box for looseconnections

Temp-5 Display reads "LO"

1/ sensor is plugged in backwards Ð reversesensor wires to controller 2/ if ambient room temperature is lower than rangeof unit Ð compare set points and ambienttemperature to rated specifications in section 8.0

Temp-6 Unit will not heat overa temperature that isbelow set point

1/ confirm that fan is moving and that amperageand voltage match data plate Ð check fan motormotion in shadow box and feel for air movement inchamber2/ confirm that set point is set high enough ,TurnHigh Limit counterclockwise and see if elementlight or High Limit light comes on3/ check connections to sensor4/ check calibration Ð using independent certifiedreference thermometer, follow instructions insection 4.5

Temp-7 Unit will not heat upat all

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1/ verify that controller is asking for heat by lookingfor element light Ð if pilot light is not oncontinuously during initial start up there is aproblem with the controller2/ check amperage Ð amperage should be virtuallyat maximum rated (data plate) amperage3/ do all controller functions work?4/ is the High Limit set high enough? Ð fordiagnostics, should be fully clockwise with the pilotlight never on5/ has the fuse at inlet blown?

Temp-8 Indicated chambertemperatureunstable

1/ ±0.1 may be normal2/ is fan working? Ð verify movement of cooling fanin center of shadow box3/ is ambient room temperature radically changing Ðeither door opening or room airflow from heatersor air conditioning? Ð stabilize ambient conditions4/ sensor miss-located, damaged, or wires may bedamaged - check mounts for control and HIGHLIMIT sensors, then trace wires between sensorsand controls5/ calibration sensitivity Ð call Customer Service6/ HIGH LIMIT set too low Ð be sure that HIGH LIMITset point is more than 5 degrees over desired Mainset point; check if HIGH LIMIT pilot is oncontinuously; turn controller knob completelyclockwise to see if problem solved then followinstructions in section 4.7 for correct setting.7/ electrical noise Ð remove nearby sources of RFIincluding motors, arcing relays or radiotransmitters8/ bad connection on temperature sensor or faultysensor Ð check connectors for continuity andmechanical soundness while watching display forerratic behavior; check sensor and wiring formechanical damage9/ bad connections or faulty capacitor Ð checkconnectors for mechanical soundness and look forcorrosion around terminals or signs of arcing orother visible deterioration

Temp-9 Will not maintain setpoint

1/ assure that set point is at least 5 degrees overambient room temperature2/ see if ambient is fluctuating

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Temp-10

Display and referencethermometer donÕtmatch

1/ calibration error Ð see section 4.52/ temperature sensor failure Ð evaluate if pilot lightis operating correctly3/ controller failure Ð evaluate if pilot light isoperating correctly4/ allow at least two hours to stabilize5/ verify that reference thermometer is certified

Temp-11

Can't adjust setpoints or calibration

1/ turn entire unit off and on to reset2/ if repeatedly happens, call Customer Service

Temp-12

Calibrated at onetemperature, but notat another

This can be a normal condition when operating temperaturevaries widely. For maximum accuracy, calibration shouldbe done at or as close to the set point temperature.

CO2 LEVEL

CO2-1 Overshoots set pointbut stabilizes

1/ purge the chamber to see if solenoid valveworks by feeling and listening to valve2/ recalibrate with Fyrite, see section 4.13 andsection 4.143/ fan not operating correctly a- fan motor stopped b- fan blade fell off c- wrong fan blade installed or mountedbackwards4/ wrong restrictor installed or missing altogether5/ tank pressure too high, see section 4.116/ CO2 sensor partially plugged with dirt orcondensation7/ regulator set wrong, see section 4.10 and 4.118/ incubator too heavily loaded9/ incubator being operated without shelving10/ CO2 attached to sample port instead of ÒCO2 INÓfitting

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CO2-2 Overshoots set pointand continues to rise

1/ debris in solenoid causing it to leak continuously2/ solenoid failed while open3/ unit miss-wired4/ CO2 sensor or interface failure5/ CO2 sensor plugged by debris or condensation

CO2-3 Rises very slowly

1/ restrictor partially plugged2/ filter overly dirty or partially plugged3/ CO2 tank regulator set too low, see section 4.114/ hose kinked or leaking5/ poor door seal6/ CO2 tank contains mixed gas, not 100% medicalgrade CO2

CO2-4 Never rises

1/ CO2 tank empty2/ solenoid failed while closed3/ restrictor plugged4/ CO2 hose blockage5/ CO2 filter plugged6/ purge timer at 0.0 and has not been reset, seesection 4.13 and 4.14

CO2-5 Is unstable Ð actualreading variesaround set point

1/ confirm that fan is working2/ check for air leak around CO2 sensor or fanmotor shaft3/ magnetic field interference4/ door not sealing properly5/ atmospheric pressure fluctuations6/ defective Fyrite pump or tubing7/ CO2 entering chamber too quickly a- tank pressure too high b- regulator set wrong c- restrictor incorrect or missing8/ top of unit exposed to cold air drafts9/ unit being operated without shadow box cover10/ incubator too heavily loaded

CO2-6 Feeding continuouslyor abnormally highCO2 usage

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1/ do decay test: if more than 1% decay in an hour,check for leak : door gasket tightness, motor inletto chamber, sensor and probe inlet to chamber ,access port not plugged2/ check solenoid valve for correct operation3/ leak in plumbing including between regulator andCO2 tank4/ door being opened too often

MECHANICALMech-1 Door not sealing

1/ check physical condition of gasket2/ confirm that door latch pulls door in tightly3/ assure that gasket is in original location

Mech-2 Motor doesn't move1/ if shaft spins freely: check connections to motorand check voltage to motor;2/ if shaft rubs or is frozen, relieve binding and retest

Mech-3 Motor makes noise1/ If noise is from the motor, tap the top of motorshaft with ball peen hammer.2/ If the sound gets worse, tap the other end of theshaft - avoiding touching the fan blade. 3/ If there is no change, call Customer Service. 4/ If noise is from shaft or fan blade, realignshaft.

Mech-4 Solenoid valvebuzzing

After removing solenoid clean with alcohol forcarbon build-up then blow out. Check valve seat orchannels for contamination. Check CO2 filter and/orgrade of CO2 used. If seat is worn, replace.

OTHEROther-1 Controller on at all

times - "locked-up"1/ turn unit off and on to reset 2/ if cannot change any condition on the front panel,call Customer Service

Other-2 Front panel displaysare all off

1/ Check for wire damage.Other-3 Unit or wall

fuse/circuit breakeris blown

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1/ check wall power source 2/ compare current draw and compare to specs ondata plate3/ see what other loads are on the wall circuit

Other-4 Unit will not turn on1/ check wall power source2/ check fuse/circuit breaker on unit or in wall3/ see if unit is on, e.g., fan or heater, and justcontroller is off 4/ check all wiring connections, esp. around theon/off switch

Other-5 Unit is smoking ÐOut of box

Put unit under vent and run at full power for onehour.

OtherÐ6

Contamination inchamber

1/ see cleaning procedure in Installation section 2.02/ develop and follow Standard operating procedurefor specific application; include definition of cleaningtechnique and maintenance schedule.

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PARTS LIST

Description 120V 220V

CO2 Filter 100199 100199CO2/Air Flow Meter 100013 100013Door Element 103068 103068Door Temperature Control 100001 100001Float Switch 210004 210004Gasket, 7 feet 3450534 3450534Glass Door 9520572 9520572Heating Element 2350503 120071High Limit Thermostat 100001 100001I/O (On/Off) Switch X1000124 X1000124Main Temperature Control 1750557 1750558Motor 4880508 4880507Pilot Light - Green 4650527 4650527Pilot Light - Red 101148 101148Potentiometer X1000699 X1000699Power Cord 104189 104192Power Inlet w/Filter and Fuse 104179 104179Purge Timer X1000698 103033Push Button Switch, Purge 103032 103032Solenoid Valve 8600528 8600529

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UNIT SPECIFICATION

Weight Shipping Net325 lbs. 295 lbs.

Dimensions Exterior WxDxH(in.)

Interior WxDxH(in.)

23x23.5x38.5 18.5x18.25x24.5

Capacity Cubic Feet4

Temperature Uniformity CO2 RangeAmb 5°C to 70°C + 0.25°C at 37°C 0 to 20%

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WIRING DIAGRAM