w ater 2016 - water institute - the water institute at unc · · 2016-05-12global water pathogen...

water2016

May 17–19

microbiology

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1Water Microbiology Conference | May 17-19, 2016

Table of Contents

WaterMicrobiology

2016

Schedule at a Glance ..............................................................................2

Detailed Schedule

Tuesday .......................................................................................4

Wednesday ................................................................................8

Thursday ...................................................................................12

Keynote Speakers ...................................................................................15

Side Event Descriptions

Tuesday ......................................................................................17

Wednesday ...............................................................................19

Thursday .................................................................................. 20

Poster Presentation Schedule

Tuesday ..................................................................................... 22

Wednesday .............................................................................. 23

Our Sponsors ............................................................................................ 24

About the Water Institute .................................................................. 26

2

Schedule at a GlanceTuesday, May 17

7:30 a.m. Registration

8:30 a.m.–noon

Break:10–10:30 a.m.

Side EventsScientific Writing: A Boot Camp

for the Writing ProcessBellflower

Global Water Pathogen Project,User Community Feedback

Redbud

State of the Science Toolsfor Water Quality Management

SESSION CANCELED

Noon–1 p.m. LunchTrillium Dining Room

1–2:15 p.m. Verbal PresentationsWindflower, Dogwood and Redbud

2:30–3:15 p.m. Keynote: Sandra McLellanGrumman Auditorium

3:15–3:45 p.m. Break

3:45–5 p.m. Verbal PresentationsWindflower, Dogwood and Redbud

5–6:30 p.m.

Poster ReceptionAtrium

Director’s ReceptionMagnolia, by invitation

5:30-6:30 p.m.

6:30–8 p.m.

( )


Wednesday, May 18 Thursday, May 19Registration Registration

Side EventsRecent Advances in Cyanobacterial Research

Windflower

Monitoring Antimicrobial Resistancein the Environment to Support Global Surveillance: How Can We Do This

to Address Emerging Concernsfor Exposure and Health Effects?

Redbud

Side EventsHow to Test, Treat and Teach

about Water in Low Income Countriesto Eliminate Waterborne Diseases

Windflower

The Use of Serum and Salivafor Identifying Waterborne Infections

in Population-Based StudiesRedbud

LunchTrillium Dining Room

AMR LunchBoardroom, by invitation

LunchTrillium Dining Room

Verbal PresentationsWindflower, Dogwood and Redbud


Keynote: David LeesGrumman Auditorium

Keynote: Jody HarwoodGrumman Auditorium

Break Break



Poster ReceptionAtrium

Mark Sobsey’s ReceptionMagnolia

5:30-6:30 p.m.

Conference DinnerTrillium Dining Room

4

Scientific Writing: A Boot Camp for the Writing ProcessBellflowerConvening Organization: Georgia State University

Contact: Lisa Casanova

Global Water Pathogen Project, User Community FeedbackRedbudConvening Organization: Global Water Pathogen Project

Contact: Amy Pruden

State of the Science Tools for Water Quality ManagementSESSION CANCELEDConvening Organizations: U.S. Environmental Protection Agency, University of North

Carolina at Chapel Hill and Southern California Coastal Water Research Project

Contacts: Rachel Noble, Sharon Nappier, John Ravenscroft and John Griffith

Side Events8:30 to 10 a.m.Bellflower and Redbud

Tuesday, May 17Detailed Schedule

Break10 to 10:30 a.m.

Side Events (continued)10:30 a.m. to noonBellflower and Redbud

LunchNoon to 1 p.m.Trillium Dining Room

Detailed descriptions of side events are provided on page 17.


ExposureWindflower

Virus Survival in Produce Under Storage and Process Environments | Carol Y. Shieh

Microbial Indicators and Pathogens in Raw Sewage, Highly Treated Reclaimed Water and Sewage Impacted Surface Waters in North Carolina: Dilemmas in Estimating Health Risks from Recreational, Agricultural and Drinking Water Use | Emily Bailey

Evaluation of Microbiological Risks Associated with Direct Potable Reuse | Jeff Soller

Water TreatmentDogwood

Chlorinated and UV-Treated Reclaimed Irrigation Water is the Source of Aeromonas Found in Vegetables Used for Human Consumption | Maria J. Figueras

Factors Influencing Point-of-Use Chlorine Disinfection Efficacy Against a Model Fecal Indicator Bacterium in Water Using Standard Performance Evaluation Testing Conditions | Robert Coleman

Characterization of the Microbiological Quality of Two Nonpotable Water Reuse Systems in Minnesota | Cheryl Haines

Recreational WatersRedbud

EPA’s Development of Recreational Water Quality Criteria for Coliphage: Updates and Coliphage Experts Workshop Overview | Sharon Nappier

A Systematic Review and Meta-Analysis of Observational Studies Evaluating the Association Between Recreational Exposure to Ambient Waters and Adverse Health Outcomes | Sorina Eftim

A Human Fecal Contamination Index for Ranking Impaired Recreational Waters Using the HF183 Quantitative Real-Time PCR Method | Orin Shanks

Verbal Presentations1 to 2:15 p.m. Windflower, Dogwood and Redbud

6

Tuesday (cont.)Detailed Schedule

Keynote: Sandra McLellanExploring the Human Microbiome

for New Indicators of Waterborne Disease Threats2:30 to 3:15 p.m.Grumman Auditorium

Break3:15 to 3:45 p.m.

Verbal Presentations3:45 to 5 p.m.Windflower, Dogwood and Redbud

BeachwaterWindflower

Beach Water Quality Modeling Using Enterococci by qPCR | Rosemarie Read

Quantification of Pathogenic Viruses and Bacteria in Stormwater Discharging to Beaches with Year-Round Surfer Populations in San Diego, California | Joshua Steele

Quantification of Pathogenic Viruses at Two Popular Southern California Surfing Beaches Impacted by Stormwater Discharge | Denene Blackwood

qPCR: A Screening Tool for Harmful Algal Blooms | Nicki Schafer


MethodsDogwood

Development and Validation of a Real-Time PCR Assay to Detect Infectious F+DNA Coliphages in Water Following Rapid Enrichment | Yvonne Yuen

Development and Evaluation of Monitoring Methods for Surveillance of Antimicrobial Resistant Fecal Indicator Bacteria in the Environment: Leon, Nicaragua, and Chapel Hill, North Carolina | Katy Brown

Incidence of Somatic and F+ Coliphage at Three Great Lake Beaches | Pauline Wanjugi

An Integrated Monitoring Approach to RTCR Level 1 and 2 Assessments | Brandon Moss

Sources and Reservoirs, ExposureRedbud

Pathogen Indicator Reduction and Bacteria Antibiotic Resistance Evaluation in Dairy Manure Separation Using Polymer | Zong Liu

Survival of Microbial Source-Tracking Markers, Pathogens and Antibiotic Resistance Genes in Poultry Litter Microcosms | Suzanne Young

Human, Wildlife and Environmental Drivers of Water Quality and Health in Chobe, Botswana | Kathleen Alexander

Efficacy Assessment of Ecological Purification System Use in Fiji | Manesha Rao

Poster Reception5 to 6:30 p.m.Atrium

Director’s Reception5:30 to 6:30 p.m.Magnolia, by invitation

8

Recent Advances in Cyanobacterial ResearchWindflowerConvening Organization: U.S. Environmental Protection Agency

Contact: Al Dufour

Monitoring Antimicrobial Resistance in the Environment to SupportGlobal Surveillance: How Can We Do This to Address Emerging Concerns for Exposure and Health Effects?

RedbudConvening Organization: University of North Carolina at Chapel Hill

Contact: Mark Sobsey

Side Events8:30 to 10 a.m.Windflower and Redbud

Wednesday, May 18Detailed Schedule


Side Events (continued)10:30 a.m. to noonWindflower and Redbud


AMR LunchNoon to 1 p.m.Boardroom, by invitation



WastewaterWindflower

Systematic Literature Reviews and Development of Distribution Curves for Viral Densities in Raw Wastewater | Sorina Eftim

Fate and Recovery of Enveloped Viruses in Municipal Wastewater | Yinyin Ye

Treatment Options for Antibiotic Resistance Genes in Wastewater | Jennifer Miller

EbolaDogwood

Ebola Virus and Surrogate Persistence and Disinfection in Wastewater | Kyle Bibby

Survival and Disinfection of Ebola Virus Surrogates in Hospital Sewage and Human Fecal Samples | Emanuele Sozzi

Disinfection of Surfaces in the Ebola Context: Efficacy Assessment of Four Chlorine Types Using E. coli and Bacteriophage Phi6 | Karin Gallandat

MethodsRedbud

Evaluation of PMA-qPCR for Quantitative Differentiation of Live Human-Associated Bacteroidales for Water Quality Monitoring | Puja Jasrotia

Propagation and Purification of Bacillus Sp. Endospores for Use | Maria Mesquita

Enhanced Detection of Poliovirus in Environmental Samples from Kenya Using the Bag-Mediated Filtration System | Nicolette Zhou


Keynote: David LeesImproving Health Controls for Viruses in Bivalve Mollusks2:30 to 3:15 p.m.Grumman Auditorium


10


Wednesday (cont.)Detailed Schedule

Antibiotic ResistanceWindflower

Characterizing Antibiotic Resistance in Singapore Water Bodies and Tributaries | Hongjie Chen

A Systematic Review on Environmental Exposure to Antibiotic Resistance Bacteria and Its Impact on Human Health with Emphasis on Methodology for Determining Linkage Between Exposure and Health Outcomes | Lydia

Abebe

The Changing Face of Water-Shifting Paradigms Linking Environmental Antibiotic Resistance to Human Health Outcomes | Clarie Sanderson

Microbial Source TrackingDogwood

Campylobacter Species, Salmonella Serotypes and Application of RNA Approach for Microbial Source Tracking at the River Kokemäenjoki Watershed | Tarja

Pitkanen

Performance Characteristics of Human Host-Associated E. coli Markers in Microbial Source Tracking in Florida | Jacob Senkbeil

Development of Cross-Assembly Phage PCR-Based Methods for Human Fecal Source Identification | Elyse Stachler

SanitationRedbud

Impact of Environmental Fecal Contamination on Hand Hygiene in Urban Harare | Tala Navab-Daneshmand

Taking Evidence-Based Microbiology to the Community Level in Lower Nyakach, Kenya, to Eliminate Waterborne Diseases | Robert Metcalf

Selection of a Biosafety Level 1 Surrogate for the Ebola Virus: Comparison of Bacteriophages MS2, M13, Phi6 and PR772m | Karin Gallandat


Poster Reception5 to 6:30 p.m.Atrium

Mark Sobsey’s Reception5:30 to 6:30 p.m.Magnolia

Conference Dinner6:30 to 8 p.m.Trillium Dining Room

Dr. Mark SobseyDuring the conference we will be celebrating

Dr. Mark Sobsey’s 50 years in environmental virology and microbiology research. Mark’s work is exemplary and his dedication to the prevention and control of water-, food- and excreta-borne disease has resulted in improved livelihoods for people throughout the world.

Mark has mentored many conference participants and inspired others to do likewise. We welcome many of his former students, post-doctoral researchers and colleagues back to Chapel Hill to celebrate this anniversary with him.

In the center of the atrium you will find the “Sobsey Lab Family Tree.” We encourage you to add yourself and others to the family tree in order to not only create a keepsake for Mark but also to document his academic lineage.

On Wednesday evening there is a private reception for Mark’s former students and colleagues in the Magnolia Lounge at 5:30 p.m. The conference dinner is also Wednesday evening and is an opportunity for us all to commemorate Mark’s 50th anniversary with a nice dinner, brief remarks and a celebratory toast.

Congratulations, Mark! Here’s to many more years of your brilliant work.

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How to Test, Treat and Teach about Water in Low Income Countries to Eliminate Waterborne Diseases

WindflowerConvening Organization: International Water and Health Alliances

Contact: Robert Metcalf

The Use of Serum and Saliva for Identifying Waterborne Infections in Population-Based Studies

RedbudConvening Organization: U.S. Environmental Protection Agency

Contact: Al Dufour

Side Events8:30 to 10 a.m.Windflower and Redbud

Thursday, May 19Detailed Schedule


Side Events (continued)10:30 a.m. to noonWindflower and Redbud




TreatmentWindflower

Analysis of Fecal Indicator Data to Identify Periods of Microbial Challenge in Drinking Water Treatment Plants | Emilie Sylvestre

Chicago Area Waterway System Microbiome Research Revealing Microbial Community Diversity and Abundance | Geeta Rijal

Inactivation and Growth of Bacterial Indicators and Pathogens in Greywater in the Context of Blue Diversion Autarky Toilet | Mi Nguyen

MethodsDogwood

Determination of Adsorption and Desorption of General Bacteroidales Genetic Marker on Freshwater and Marine Sediments by Quantitative Real-Time PCR | Jia Xue

Oysters and Clams Collected from the Same Location Exhibit Marked Differences in Vibrio Colonization | Brett Froelich

Delineating the Microbial Ecology of Phage-Prokaryotes in Hypersaline Environment through “Omics” Approach | Amir Mohaghegh Motlagh

E. coliRedbud

An Evaluation of E. coli Quantification in Compartment Bag Tests Incubated at Multiple Temperatures | Patricia Akers

EPA Method 1603 “Spike the Ball” Study | Rosemarie Read

Rapid Detection of E. coli in Eight Hr from Beach Water Using KwikCount EC Medium

| Fu-Chih Hsu


Keynote: Jody HarwoodLife and Death in a Tough Spot:

Persistence of Fecal Microbiota in the Environment2:30 to 3:15 p.m.Grumman Auditorium

14

Thursday (cont.)Detailed Schedule



MonitoringWindflower

Monitoring for the Human Parasitic Nematode Enterobius vermicularis in Municipal Wastewater Effluent in Alberta, Canada | Sydney Rudko

Make it “Tough:” qPCR Master Mix Comparison | Nicki Schafer

Impact of Urbanization on Physico-Chemical and Microbiological Characteristics of Canals in Suzhou, China | Sekar Raju

Methods, Management and Treatment Dogwood

Eco-Genomics of Temperature Effects on Partial Nitritiation/Anammox (PN/A) Reactor | Ananda Bhattacharjee

Metagenomics Approach for Comparative Study of Microbial Community Structure in Granular and Conventional Activated Sludge | Sunaya Dasgupta

Metagenomic Characterization of Vibrio in the Neuse River Estuary, North Carolina | Kelsey Jesser

RiskRedbud

Health Risks Associated with Integrated Aquaculture in Rural China: Focusing on Zoonotic Pathogen and Antibiotic Resistance Hazards | Jiyoung Lee

Spatio-Temporal Dynamics of F-Specific RNA Bacteriophages in River Water During Rainfall-Runoff Events: New Data for Water Quality Risk Assessment | Blandine Fauvel


Keynote SpeakersSandra McLellan, PhDProfessor in the School of Freshwater Sciences, University of Wisconsin-Milwaukee

Dr. McLellan’s research focuses on studying microbial communities in the urban environment and the implications for human and ecosystem health. Her laboratory employs next generation sequencing approaches to identify new indicators of pollution and pollution impacts. Dr. McLellan’s group uses these indicators to determine the causes of beach closings on the Great Lakes.

She graduated from the University of Wisconsin-Milwaukee in Health Sciences in 1990 and worked at Miller Brewing Company before entering graduate school. Dr. McLellan earned her graduate degree in Environmental Health at the University of Cincinnati College of Medicine in 1998.

David Lees, PhDChief Food Safety Advisor, Centre for Environment, Fisheries, & Aquaculture Science

Dr. Lees has more than 30 years experience in human health microbiology, including disease diagnosis and monitoring, virological research, algal biotoxin monitoring, statutory related programs and reference laboratory activities. He is the senior food safety advisor in the Food Safety group at the Cefas Weymouth laboratory, U.K. The Food Safety group covers activities associated with; European Regulations on food safety—classification of bivalve mollusk harvesting areas, approval of shellfish purification plants, sanitary surveys and monitoring for algal biotoxins; advice on sewage discharges; and associated applied research on algal biotoxins, fecal pollution of the marine environment and enteric viruses impacting seafood. David advises the U.K. government and European Commission on issues related to bivalve mollusk safety. Cefas Weymouth is the designated U.K. National and European Union Reference Laboratory (EURL) for bacterial and viral contamination of bivalve mollusks. Dr. Lees is director of the EURL.

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Valerie J. (Jody) Harwood, PhDProfessor and Chair, Department of Integrative Biology, University of South Florida

Dr. Harwood is an environmental microbiologist whose research focuses on microbial ecology, water quality and the use of molecular biology tools for assessing the extent and sources of fecal contamination in water. She is a major contributor to the U.S. EPA Guide Document on microbial source tracking (MST) and is a co-editor of the book “Microbial Source Tracking: Methods, Applications & Case Studies” (Springer 2011). She has also conducted extensive research on the persistence and ecology of enteric organisms in secondary habitats such as water and sediments. Dr. Harwood is at the forefront of efforts to implement quantitative PCR methods as a tool in environmental microbiology research.

She is the author of over 80 peer-reviewed publications on subjects including MST, environmental persistence and fate of fecal indicators and waterborne pathogens, and Vibrio vulnificus ecology. She collaborates with agencies ranging from local to international (e.g., U.S. EPA, WHO, UNESCO) on water quality issues. She is a member of the Council Policy Committee (executive board) and a Distinguished Lecturer for the American Society for Microbiology, as well as a Fulbright Specialist Award recipient for work in Queensland, Australia, with the Commonwealth Scientific and Industrial Research Organisation.


Writing up your work for publication can be one of a scientist’s biggest challenges. Your career advancement, and the advancement of your field, depends on getting your work into the peer-reviewed literature. Do you have projects you need to write for publication? Does the writing process seem overwhelming and intimidating? Are you struggling with organization and presentation of your ideas? Do you have writing in progress that isn’t coming together the way you want? Whether you are just starting research or ready to write, this interactive workshop will help you make progress on your paper with a framework for organizing your writing and help you work toward the goal of a publishable paper by tackling all stages of your writing process:

• What are my writing goals?• How do I focus part of a large project into a clearly defined manuscript? • How is a scientific paper structured?• How do I organize my paper around my main research goals?• What goes in each section?• How do I organize the presentation of data?• Where do I start in writing a discussion? • How do I place my work in a larger context?• How does the process of publication work? What can I expect?Bring your questions and manuscript writing projects. This workshop is highly interac-

tive—we will work with your specific examples to help you organize and plan your writing. This workshop is for scientists at any stage of the writing process, whether you are just start-ing or in the process of writing a manuscript.

Side Event DescriptionsTuesday, May 17Scientific Writing: A Boot Camp for the Writing Process | BellflowerConvening Organization: Georgia State UniversityContact: Lisa Casanova

Global Water Pathogen Project (GWPP), User Community Feedback | RedbudConvening Organization: Global Water Pathogen ProjectContact: Amy Pruden

Although clean water and sanitation are a human right, the combination of inadequate sanitation, poor hygiene and unsafe drinking water is responsible for an estimated annual burden of 2 million diarrheal deaths. The World Health Organization (WHO) reported in 2014 that 1.8 billion people use a drinking water source contaminated with feces. Almost 85% of the world’s total wastewater is discharged without adequate or any treatment. Climate change is aggravating this situation. In response, adequate management

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of wastewater must address the risk factors constituted by water pathogens. Sanitation and Disease Health Aspects of Excreta and Wastewater Management (Feachem et al. 1983) currently remains the most comprehensive overview of fecal indicator and pathogen occurrence, characteristics, development, control and dissemination in the environment. Since its publication it has played a key role in the prevention of incidence and mortality associated with water-related diseases. However, its content requires updating because of the dramatic increase of relevant knowledge and data over the past 30 years.

The objective of the GWPP is to improve sustainable access to safe drinking water and sanitation by updating knowledge on water pathogens using advanced information technologies. This will be accomplished by publishing and disseminating a state-of-the-art reference book on water-related disease risks and intervention measures (replacing Feachem et al. 1983) and creating an online open-access database and knowledge platform. The updated reference and online database will provide an updated review of the efficacy of sanitation technologies and serve as a compendium of waterborne pathogen information and quantitative data to support risk assessment to protect water safety. The GWPP is also coordinating with the WHO to support its Sanitation Guidelines.

The GWPP began in May 2014 with completion slated for December 2017. The GWPP network is conducting workshops and poster sessions and setting up booths at conferences and other venues worldwide to connect with the user community from professional, civil society and political sectors and to solicit feedback on the reference book and online database.

This workshop will comprise a brief introductory presentation, hands-on demonstration of the online tool and example quantitative microbial risk assessment case study using the reference book or online tool. User feedback will help in the implementation of the GWPP deliverables, i.e., helping policy, regulators and practitioners to use the GWPP deliverables as resources in their work. The target audience is conference attendees from CDC, U.S. EPA, state public health agencies, wastewater municipalities, water reuse entities, academia and consultants.

State of the Science Tools for Water Quality Management | SESSION CANCELEDConvening Organizations: U.S. Environmental Protection Agency, University of North Carolina at Chapel

Hill and Southern California Coastal Water Research ProjectContacts: Rachel Noble, Sharon Nappier, John Ravenscroft and John Griffith

The water quality microbiology research community can benefit from knowledge of current tools that are available for implementation for improved management of coastal and receiving water quality. Since the release of the new criteria document for recreational waters by U.S. EPA in 2012, multiple new tools and criteria are in various phases of development. Included in those are 1) the use of coliphage for management of coastal waters, particularly those impacted by wastewater discharges, 2) quantitative microbial risk assessment and 3) natural source exclusion. In this exciting time of tool and new criteria development, there is much that the water microbiology research community can contribute


through the identification of key data gaps relating to successful implementation. This side event will promote a dialogue among developers of these tools, scientists working at the forefront of the respective fields, the research community and managers. The goal of this side event is to conduct a highly technical dialogue on the issues surrounding each topic, and to identify high priority data gaps for each new tool that can advance the field. While the dialogue will be focused on recent activities in the United States, attendees from different regions of the globe are welcome and desired to promote the advancement of the science.

Wednesday, May 18Recent Advances in Cyanobacterial Research | WindflowerConvening Organization: U.S. Environmental Protection AgencyContact: Al Dufour

Cyanobacteria and their toxins are receiving increased interest because of the potential severity of the effects of their toxins and because of the increasing reports of dermal, respiratory and gastrointestinal effects in recreationists exposed to surface waters. It is the purpose of this side event to bring to the attention of the scientific and policymaking communities some of the recent advances in: molecular toolbox development that can detect and identify cyanobacterial targets linked to toxin production; the identification of allergenic peptides and their functional interaction with cyanobacterial toxins; health effects associated with cyanobacterial toxins; and descriptions of the diversity of cyanobacteria in freshwater ponds.

The presentations at this side event will introduce some new approaches to identifying risk associated with cyanobacteria.

Monitoring Antimicrobial Resistance in the Environment to Support Global Surveillance: How Can We Do This to Address Emerging Concerns for Exposure and Health Effects? | Redbud

Convening Organization: University of North Carolina at Chapel HillContact: Mark Sobsey

It is now well documented that antimicrobial resistant bacteria (ARB) and their genes are common and widespread in water and other environmental media, including wastewater and human excreta used for agriculture and discharged to the environment. Many of these bacteria are resistant to multiple antibiotics. There is considerable overuse, careless use, inappropriate use and unregulated availability and use of many antibiotics in medicine, animal treatment and veterinary medicine. There is also extensive and largely unregulated use in animal agriculture and aquaculture. This overuse and abuse contributes to the extensive presence of antibiotic compounds, their degraded compounds and metabolites and ARB bacteria in water, soil, sediments and across aquaculture and animal production food production facilities. Hotspot sources of ARB such as hospital

20

and community sewage, animal manures from intensive livestock operations, water of intensive aquaculture systems and pharmaceutical waste discharges are recognized but rarely if ever monitored to support ARB surveillance at a local, national or global scale. Such monitoring is now considered essential and needs to be harmonized with ARB surveillance in healthcare and agricultural/veterinary systems.

Greater and more strategic and focused efforts are needed to bring experts and various stakeholders together to identify candidate methods for monitoring ARB and their genes in environmental media, especially in hotspot sources and environments impacted by these sources to which humans and animals may be exposed. Such ARB monitoring is essential for assessing status and trends and evaluating new and improved efforts to eliminate or control these sources using integrated management systems.

This side event’s purpose is to present the WHO, the EU Joint Monitoring Program for Antimicrobial Resistance, U.S. government agencies (U.S. EPA, USDA, FDA and CDC) and others with recommendations, guidance and suggested specifications, as well as promote the concepts and materials for future expert workshops and implementation programs and activities to further develop the monitoring system. Working group members will be identified to take the initiative forward with participating stakeholder groups.

Thursday, May 19How to Test, Treat and Teach about Water in Low Income Countries to Eliminate

Waterborne Diseases | WindflowerConvening Organization: International Water and Health AlliancesContact: Robert Metcalf

The world largely ignores the chronic epidemic of waterborne diseases among more than 1 billion people living in low income countries that WHO estimates daily leads to more than 4.6 million people getting diarrhea and more than 2,000 people dying from waterborne diseases. Unlike “the big three”—HIV/AIDS, malaria and tuberculosis—waterborne diseases are easily preventable: kill the germs with heat or chlorine, and people don’t get sick.

As microbiology is the key to understanding waterborne diseases, microbiologists are uniquely positioned to take the lead in raising awareness of this chronic epidemic and in presenting practical strategies that low income communities could use to eliminate waterborne diseases.

The first part of the workshop will cover two practical water testing methods currently used in low resource areas: 1) the Portable Microbiology Laboratory (PML), a field kit that contains two tests widely used in the water and food industries to detect the fecal indicator E. coli, and 2) the Aquagenx portable and self-contained Compartment Bag Test (CBT) for E. coli. Workshop participants will learn how to perform these tests on water sources to obtain next day results that correlate with WHO disease risk guidelines. The second part of the workshop will introduce disinfection methods, their strengths and weaknesses. The


last part of the workshop will present the ongoing Friends of the Old (FOTO) Goal is Zero project in Lower Nyakach, Kenya. This is an example of how evidence-based testing, community education and provision of disinfection methods (liquid chlorine or solar water pasteurization) have enabled the 70,000 citizens of Lower Nyakach to render waterborne diseases negligible. The FOTO project is easily replicable.

The Use of Serum and Saliva for Identifying Waterborne Infections in Population-Based Studies | Redbud

Convening Organization: U.S. Environmental Protection AgencyContact: Al Dufour

The purpose of this side event is to bring to the attention of the scientific community new approaches for identifying infections in large populations, which enhance our ability to define burden of disease and identify etiological agents associated with recreational and drinking water exposures. The use of antibody biomarkers, noninvasive specimen collection and experimental designs for collecting pathogen data will be discussed. Research examples will include: description and use of the multiplex bead assay to monitor changes in infection prevalence over time; discovery of etiological agents of disease related to swimming-associated exposures; and identifying pathogens associated with drinking water exposures. The goal of this side event is to educate and stimulate the interests of attendees as to the value of new approaches for identifying etiological agents and defining burden of disease, and to encourage the free exchange of ideas among scientists.

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Poster Presentation ScheduleTuesday, May 17

Title Presenter

Opportunistic Pathogens in a Hot Water Pipe Loop SystemNicole Rockey,

University of Michigan

Nontuberculous Mycobacteria in Point-of-Use Water Sources in

Homes: A Case-Control Study of Association with Human Disease

Connie Zhou,

University of Washington

A Potential Role for Cations in Aquatic Microbiology

in Urbanizing Watersheds

Brian Badgley,

Virginia Tech

Antibiotic Resistance Characterization of Surface Water Bacteria

in an Urbanizing Watershed

Dylan Laird,

Texas A&M University

Characterizing Rotavirus A from Water Samples in Nairobi, KenyaJill Falman,


Comparative Decay Kinetics of Fecal Indicator Bacteria, Host-

Associated Fecal Markers and Pathogens in a Coastal California

Lagoon

Laurie Van De Werfhorst,

University of California–Santa

Barbara

Correlating the Relative Abundance of Ammonia and Nitrite

Oxidizing Bacteria with the Nitrification Performance Using

Hierarchical Oligonucleotide Primer Extension (HOPE)

Peiying Hong,

King Abdullah University of

Science and Technology

Comparison of the qPCR and the HybriScan Legionella Assays Vs.

Culture for the Identification of Legionella Sp. in Nonpotable Water

Samples

Rosemarie Read,

Northeast Ohio Regional

Sewer District

Feasibility of Escherichia coli as an Indicator of Electrochemical

Disinfection with a BDD Anode

Rosa Maria Araujo Boira,

Universitat de Barcelona

Environmental Surveillance of Polio in Kenya: Key Lessons LearnedJ. Scott Meschke,


Detection of Waterborne Enteropathogens from a River Water

Sample to Trace the Source of Contamination

Sarmila Tandukar, Tribhuvan

University Teaching Hospital

Optimal Strategies for Monitoring Irrigation Water Quality

and the Development of Guidelines for the Irrigation of Food Crops

Marc Verhougstraete,

University of Arizona


Title Presenter

Opportunistic Pathogens in a Hot Water Pipe Loop SystemNicole Rockey,

University of Michigan

Nontuberculous Mycobacteria in Point-of-Use Water Sources in

Homes: A Case-Control Study of Association with Human Disease

Connie Zhou,


A Potential Role for Cations in Aquatic Microbiology

in Urbanizing Watersheds

Brian Badgley,

Virginia Tech

Antibiotic Resistance Characterization of Surface Water Bacteria

in an Urbanizing Watershed

Dylan Laird,


Characterizing Rotavirus A from Water Samples in Nairobi, KenyaJill Falman,


Comparative Decay Kinetics of Fecal Indicator Bacteria, Host-

Associated Fecal Markers and Pathogens in a Coastal California

Lagoon

Laurie Van De Werfhorst,

University of California–Santa

Barbara

Correlating the Relative Abundance of Ammonia and Nitrite

Oxidizing Bacteria with the Nitrification Performance Using

Hierarchical Oligonucleotide Primer Extension (HOPE)

Peiying Hong,

King Abdullah University of

Science and Technology

Comparison of the qPCR and the HybriScan Legionella Assays Vs.

Culture for the Identification of Legionella Sp. in Nonpotable Water

Samples

Rosemarie Read,

Northeast Ohio Regional

Sewer District

Feasibility of Escherichia coli as an Indicator of Electrochemical

Disinfection with a BDD Anode

Rosa Maria Araujo Boira,

Universitat de Barcelona

Environmental Surveillance of Polio in Kenya: Key Lessons LearnedJ. Scott Meschke,


Detection of Waterborne Enteropathogens from a River Water

Sample to Trace the Source of Contamination

Sarmila Tandukar, Tribhuvan

University Teaching Hospital

Optimal Strategies for Monitoring Irrigation Water Quality

and the Development of Guidelines for the Irrigation of Food Crops

Marc Verhougstraete,

University of Arizona

Wednesday, May 18

Title Presenter

Rapid and Efficient Extraction Method of Chlorophyll by Bead

Beating

Nicki Schafer, Northeast

Ohio Regional Sewer District

Actinobacteria Isolated from Deep-Water Endemic Amphipods of

Lake Baikal as a Source of Novel Biological Active Compounds

Evgenii Protosov,

Irkutsk State University

Exploration of Strategies to Track the Downstream Impacts of

Human Sewage in Underserved Rural Communities

Jacob Cantor,

Virginia Tech

Microbiological and Physico-Chemical Characteristics of Surface

Water Collected from the Tiaoxi River (Taihu Watershed), China

Sekar Raju, Xi’an Jiaotong-

Liverpool Universityy

Patterns of Antimicrobial Tesistance in E. coli Isolates from Soil and

Water Samples in Three Texas Watersheds with Different Land Uses

Maitreyee Muherjee,


Microbe-Suspended Sediment Dynamics of Recreational Beach

Water of the Chesapeake Bay

Jon Calomiris,

Sotiria Science

Development of a One Health Sampling Scheme for Surveillance of

Antimicrobial Resistant Bacteria in Northern Colorado

Megan Dietz,

Colorado State University

Water Quality Testing for Bacterial IndicatorsKatherine Vilardi, Wentworth

Institute of Technology

Water Quality and Fecal-Indicator Detection in Response to an

Impaired Urban Watershed, Turkey Creek: A “Gulf of Mexico

Initiatve Focus” and a “Making a Visible Difference” Program

Stephanie Friedman,

EPA/ORD/NHEERL/GED

Handwashing for Ebola Outbreak Contexts: Comparison of Soap,

Hand Sanitizer and 0.05% NaDCC, HTH and NaOCl Chlorine

Solution on the Inactivation and Removal of Model Organisms

Marlene Wolfe,

Tufts University

Fate and Transport of Fecal Indicator Bacteria and Microbial Source-

Tracking Targets in GroundwaterHannah Billian, Virginia Tech

Meta-Analysis of Seasonal Concentration and Flow-Based Results

for Escherichia coli and Bacteroidales for Inland Recreational Waters

from Middle Tennessee

Megan Stallard,

Middle Tennessee State

University

Exposure Assessment of Livestock Carcass Management Options

for Natural Disasters

Kaedra Jones,

ICF International

24

Our SponsorsGOLD

Bio-Rad’s Life Science Group develops, manufactures and markets a wide range products used for research in functional genomics, proteomics and

food safety. The group ranks among the top five life science companies worldwide, and maintains a solid reputation for quality, innovation and a longstanding focus on the success of its customers. Bio-Rad’s life science technologies include electrophoresis, imaging, multiplex immunoassay, chromatography, microbiology, bioinformatics, protein function analysis, transfection, flow cytometry and nucleic acid amplification. Bio-Rad’s Digital Biology Center specializes in the development of advanced technologies for the rapidly growing field of digital biology—including complete solutions for droplet digital PCR and digital sequencing.

SILVERThe NSF Applied Research Center (ARC) is NSF International’s research and development arm, providing custom R&D services. Services include expert testing, method development, product validation/commercialization and risk assessment. The ARC furthers NSF’s mission of public health and safety by supporting scientific innovation and leadership.

Our independent laboratory and consultation services include: microbial control and antimicrobial efficacy claims; trace analyte assessment; human health risk assessments; consulting for GLP, GMP, lab design and method training; and genomic and bioinformatics services.

Scientific Methods provides microbiologic testing, specialized in human enteric viruses

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BRONZEThe Aquagenx Compartment Bag Test (CBT) is a simple, portable water quality test kit

that lets anyone, anywhere determine if drinking water contains E. coli bacteria and poses a health risk. It is ideal for on-site testing in low resource and disaster settings because it generates quantified test results without requiring electricity, labs or extra equipment such as incubators and UV lamps. The CBT’s simplicity and convenience remove the barriers for bacteriological water quality monitoring in any location where testing was previously too difficult or costly.

InnovaPrep—Automated concentration of bacteria, protozoa, viruses or other

microorganisms from large liquid volumes. See a demonstration of the Concentrating Pipette—the first truly automated liquid to liquid concentrator.

26Water

Microbiology

2017

Save the date!

May 15-19, 2017 #UNCwatermicro

About UsThe Water Institute at UNCThe Water Institute at UNC provides global academic leadership for sustainable management of water for health and human development. Based in UNC’s Gillings School of Global Public Health, one of the highest ranked public health schools, we work to solve the complex global water challenges through our four main strategic functions: research; teaching and learning; knowledge and information management; and networking and partnership development. We have seven focus areas to support these functions, which are: monitoring, valuation and learning for WaSH; WaSH governance; sanitation for the 21st century; adapting to water scarcity and climate change; drinking water for all; national and regional WaSH challenges in the U.S.; and the water-food-climate-energy nexus.

Through all our endeavors, we work toward improving access to safe water, sanitation and hygiene for all. We unite faculty, students, practitioners and policymakers around this common purpose on a local, national and international scale.

Concurrent with the 2017 Water Microbiology Conference will be the 2017 IWA Health Related Water Microbiology Conference, also hosted by The Water Institute at UNC. IWA participants will be able to participate in all Water Microbiology Conference sessions as well as several IWA only sessions.

Both conferences will include the following, selected from submitted abstracts and proposals:

• Side event sessions• Verbal presentations• Poster presentationsThe IWA conference also will contain networking events

and opportunities for arranged visits and tours of labs and other sites of interest. Information regarding abstract and proposal submission, as well as registration fees, will be announced in mid-2016.

The host committee for the joint conference is under the leadership of Drs. Mark Sobsey, Jamie Bartram, Rachel Noble and Jill Stewart.

16-2038

To learn more, visit bio-rad.com/QX200

Take water quality testing to the next level

Quantify small fold changes in target DNA or RNA with extreme precision using Bio-Rad’s QX200™ Droplet Digital™ PCR technology. With a greater tolerance to PCR inhibitors compared to qPCR, ddPCR™ affords unparalleled diagnostic sensitivity and reproducibility.

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The QX200 Droplet Digital PCR system and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. Purchase of the product includes a limited, non-transferable right under such intellectual property for use of the product for internal research purposes only. No rights are granted for diagnostic uses. No rights are granted for use of the product for commercial applications of any kind, including but not limited to manufacturing, quality control, or commercial services, such as contract services or fee for services. Information concerning a license for such uses can be obtained from Bio-Rad Laboratories. It is the responsibility of the purchaser/end user to acquire any additional intellectual property rights that may be required.

Please contact your local Bio-Rad representative for additional information.

Applied Research Center (ARC)Public Health Research and Validation

NSFresearch.org

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CLAIMSVALIDATION

PROOF OFCONCEPTTESTING

METHODDEVELOPMENT

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ARC - UNC Water Ad_PRINT.pdf 1 4/20/2016 8:58:12 AM

Scienti�c Methods Inc

Providing microbiologic testing; specialized in human enteric viruses by infectivity assay and RT-qPCR,

validation of new methods and new products, providers of the Continuous Flow Centrifuge and positive charged �lters for multiple pathogen concentration, and

easy-to-use, �eld ready kits to quantify coliphages and rapid enumeration of E. coli.

Our Sponsors

w ater 2016 - water institute - the water institute at unc · · 2016-05-12global water pathogen...

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