vmu policy sop manual v-2009-07

8/10/2019 VMU Policy SOP Manual v-2009-07

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VA LONG BEACH HEALTHCARE SYSTEMRESEARCH HEALTHCARE GROUP

VETERINARY MEDICAL UNIT

POLICIES AND PROCEDURES MANUAL

(Version 07/200!


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DAILY MANAGEMENT PROCEDURE

Each animal room is to be physically inspected daily for the following items:

1. Animal Condition

A. Observe each animal for any signs of illness or injury. !efer to your animal condition informationsheet." !eport any illness or injury to supervisor.

#. $hould a dead animal be found

1" Chec% investigator protocol for any special instructions. &ollow special instructions' if applicable.

2" !outine procedure in lieu of special instructions.

a" !emove animalb" (lace in plastic bag) sealc" *abel with investigator+s name and dated" (lace in carcass disposal free,ere" -otify investigator by email or phone

2. Cage Condition

A. Chec% that cage cards are properly in place.

#. Chec% that cages are sanitary. Any unusually soiled cages must be echanged. !eport any unusualconditions to supervisor.

C. Chec% water supply.

1" Automatic systems / chec% proper operation) free/flowing outlets' ade0uate pressure' no lea%age.

2" ater bottles / minimum 13 full' free/flowing outlet' no lea%age.

3" ater bowls / minimum 13 full and clean.

4. Chec% food supply.

3. !oom condition

A. Observe overall condition !efer to 4aily !oom Chec% $heet". !eport any needed repairs or unusualconditions to supervisor.

#. Chec% air temperature. 5he temperature should be 678/ 928. !eport any variance to supervisorimmediately.

C. Chec% automatic light timers.

4. Empty refuse container.

E. $weep and mop floors.

. Complete room chec% record form

STANDARD OPERATING PROCEDUREVETERINARY MEDICAL CARE

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Ade0uate veterinary medical care is provided for all animals maintained for research' testing' training' oreducational purposes. 5he program of veterinary medical care is and monitored by a laboratory animalveterinarian 0ualified by training and eperience for this responsibility.

5he program includes:

1. &re0uent observation of animals by a person 0ualified to verify the health of each

animal.

2. (rovision of veterinary medical care for animals found to be ill or injured.

3. Application of currently accepted measures of prophylais and therapy.

. Consideration for humane aspects of animal eperimentation such as the proper use ofanesthetics' analgesics' and tran0uili,ers and the implementation of such measures as directedby the responsible veterinarian to alleviate unacceptable levels of pain or distress to animalsubjects.

;f an animal is found and in need of veterinary medical care the animal careta%er will call the consult veterinarianor the on call veterinarian to determine the course of care the animal will need. An emergency call list for theveterinarian is located on the door of the office.

STANDARD OPERATING PROCEDUREDAILY CLEANING PROCEDURE

=ice

1. Cage bedding is cleaned every 3rddaya. Eceptions


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i. (ups #orn1. wait at least ?/9 days to clean

b. 5ransfer mice to clean cagec. Clean enhancement tubes as neededd. 4irty cages are emptied into trash in dirty room' rinsed and placed on rac%e. !un dirty cages through cage washer and store clean cages in clean room

2. Cage tops and bottles are cleaned every 2 wee%sa. Cage tops are cleaned in cage washer

b. #ottles and tops are cleaned in bottle washer3. !ac%s

a. !un through cage washer as needed

!ats

1. &ollow mice procedure

!abbits

1. !abbits re0uire fresh water and food daily2. !abbits are housed in cages operating on an automatic watering system

3. $oiled bedding pan is cleaned every 3rd

day. ash bedding pans on rac%?. !un through cage washer on rabbit cycle6. Entire rac% should be cleaned at least once a wee% in the cage washer

STANDARD OPERATING PROCEDURECLEANING CAGES "HEN CAGE"ASHER IS DO"N

5his procedure is to be followed when the cagewasher is down for repairs or maintenance and cagesneed to be washed by hand.

(rocedure

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4ispose of bedding

>sing a hose remove any ecess bedding left in cage

4ip cages in 1@ bleach solution

!inse with water

$oa% cages for 1@ minutes in *pB dilute according to manufacturer+s recommendation"

!inse with water

(lace cages in autoclave for 3@ minutes on 17@ degree temperature !emove from Autoclave

(lace cages on wash rac% to dry on clean side of cage wash area

Once cages are dry approimately 1?/2@ minutes"' fill with clean bedding

(lace clean cages on clean cart and cover

STANDARD OPERATING PROCEDUREOPERATING THE AUTOCLAVES

5he is e0uipped with two autoclaves. One located in #17a in the surgery suite. 5his autoclave isused to sterili,e surgical instruments or surgical supplies for surgery. 5he other autoclave is located in#31. 5his autoclave is used to sterili,e large loads of cages' bedding' etc. 5his autoclave is set up toload dirty materials on the dirty side located in #27 dirty side of cagewasher" and the clean materialsare unloaded on the clean side located in #31".

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5o operate the autoclave in the surgery suite:

1" *oad the instruments or surgical supplies on the tray2" Close the door and secure tightly3" $elect cycle: dry' fluid' " Adjust time' temperature' etc. if necessary?" (ress start

WARNING: hot steam may escape if door is fuy ope!ed" Wear appropriate ##E $he! cham%erco!te!ts are hot"

5o operate the autoclave located in #31:

1" *oad materials on trays"2" Close door and secure tightly3" $elect cycle: fast' dry' fluid" Adjust time' temperature' etc if necessary?" Eit out of menu until prompt bring you bac% to main menu6" Enter 3 digit code any numbers will do"9" (ress enterrun

WARNING: hot steam may escape if door is fuy ope!ed" Wear appropriate ##E $he! cham%erco!te!ts are hot"

STANDARD OPERATING PROCEDUREANIMAL CONDITION IN#ORMATION SHEET

5he following items must be considered when chec%ing animal condition:

1. Deneral physical conditiona. 4ebilitated

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b. 4ehydratedc. Emaciatedd. >nthriftye. Obesef. Comatoseg. Abnormal breathing

2. Evidence of injury non/specific"

a. *impingb. (aralysisc. Ataiad. 4ilatation of pupilse. Convulsionsf. *imb fractureg. (ost/surgical complicationh. Bemorrhagei. ounds or contusionsj. 5umors

3. #ehavior

a. Changed e.g.' friendly to aggressive"b. Aggressivec. (hlegmaticd. Ballucinations

. Coat or featheringa. >n%emptb. =atted especially in perianal regions"c. Alopeciad. !uffled hair or feathering

?. ;ntegumenta. $cabs or crusts

b. Abrasionsc. oundsd. (ustulese. #listersf. Ec,ema / moist or dryg. ;nflammationh. 5umorsi. >lcerations

6. Evidence of middle andor inner ear damagea. Bead tiltb. $pinning when suspended by tail rats and mice"

c. !unning in circlesd. !otating

9. Oral cavitya. (ale gumsb. ;nflamed gumsc. #leeding gumsd. =alocclusion of the mandiblee. &oul odor from mouthf. #listers on lips or tongueg. Oral tumors

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h. Overgrown teethi. 4ental tartar calculus"j. Abscessed teeth%. !etained deciduous teethl. Cavities

7. Eyesa. 4ischarge

1" #ilateral2" >nilateral

b. Opacity1" #ilateral2" >nilateral

c. 4ilation of pupils fied"d. *esionse. ;nflammation of conjunctiva

. -aresa. 4ischarge

1" #ilateral

2" >nilateralb. *esions

1@. Earsa. 4ischarge

1" #ilateral2" >nilateral

b. Crust or scabs in canalc. Open woundsd. Bematomas

11. Ano/genital areaa. Evidence of diarrhea

b. Abnormal dischargesc. *esions

12. $cratching possible ectoparasite infestation"

13. Bead sha%ing possible ectoparasite infestation"

1. &orepaws matted with discharge wiped from nose andor eyes


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STANDARD OPERATING PROCEDURE

CAGE CONDITION IN#ORMATION SHEET

5BE &O**O;-D ;5E=$ =>$5 #E CO-$;4E!E4 BE- CBECF;-D CADE CO-4;5;O-:

1. Evidence of diarrhea

2. Evidence of constipation

3. #lood in the feces

. orms in the feces

?. *ac% of urine

6. Ecessive urine

9. #lood andor eudates from lesions" plastered on cage walls.

7. Evidence of anoreia food untouched or not eaten"

. *ow water consumption

1@. Ecessive water consumption

11. Evidence of vomiting

12. orms in vomitus

13. #lood in vomitus

1. ;nterior cage damage especially sharp edges"

1?. Eterior cage damage

16. (opulation per cage in ecess of regulations.

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ROOM CONDITION IN#ORMATION SHEET

5BE &O**O;-D ;5E=$ =>$5 #E CO-$;4E!E4 BE- CBECF;-D !OO= CO-4;5;O-:

1. Cleanliness

a. &loorsb. allsc. $in%sd. Counter topse. !efuse containersf. Cage rac%sg. E0uipment including buc%ets and mops"h. &ood containersi. #edding supplies

2. =echanical operation

a. 5emperatureb. Automatic watering systemc. Automatic lighting systemd. =anual lights

3. Evidence of pests

. Ecessive odor


PROGRAM #OR MONITORING THE HEALTH STATUS

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$entinel mice and rats are used to monitor the health status of animals in our facility. $entinel mice andrats are purchased twice a year. >pon arrival of a new group or micerats' one animal of each species iseuthani,ed for necropsy' parasitology' and serology. 5he remaining new arrivals are 0uarantined until lab resultsare received verifying the new arrivals are clean. 5hereafter' every three days when soiled bedding is changed forthe facility rodent population' approimately one/half ounce per cage" of soiled bedding is mied with thesentinel micerat fresh bedding. $entinels are monitored for any appearance of illness. 5here is one cage ofsentinel animals per every room of rodents. One sentinel animal per room is given necropsy and has serology and

parasitology performed four times a year.


BREEDING

=ice and !ats

=ice and rats can become seually mature at 6 wee%s of age

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Estrus occurs in the female about every days and immediately after parturition

Destation is usually 1/21 days

;t is recommended by the that ;nvestigators label breeding cages by mar%ing a start date

=ultiple females can be housed with males but it is recommended by the to remove pregnant

females to avoid multiple litters

;t is recommended by the that males be removed once females are pregnant but not a re0uirement

;t is highly recommended that ;nvestigators observe breeding colonies daily for signs of pregnancy

-estling pads are placed in cages for pregnant mice (lease read the attached recommendations from the Gac%son *aboratory regarding standard breeding

suggestions

STANDARD BREEDING SUGGESTIONS ($%&'son L%or%)or*!

1. ;f the rac%s are against the wall the vibrations can disturb the mice' so you may want to move the rac%s acouple of inches away from the wall.2. =a%e sure the mice are not located near a door or sin% where there may be heavy traffic or loud noises. !emindcareta%ers to wor% gently' slowly and 0uietly when handling the mice.

3. Changing the cages more than 1/2 times a wee% may cause the mice to get agitated / resist the temptation toconstantly chec% the mice.. >se gloves or forceps sterili,e glovesforceps between cages". #e aware of unnatural odors toic fumes'perfume" as these can adversely affect your mice.?. ;f a female is ready to deliver' s%ip changing cage until the pups are 2 days old or mil% spots are visible in thepupsH tummies.6. ;f the female does not nest the pups up right away after birth' but leaves them scattered around the cage' donHtgive the benefit of the doubt/ foster them out.9. *eave males in with females at all times. !emoving them from the cage can cause the mom to become stressedand abort or cannibali,e the pups.7. 5ry using nestlets or %imwipes for nesting material.. Chec% the temperature in your mouse room' I67/92 is optimal range of

6?/9? is tolerable"1@. Chec% your light cycle. e use a 1 hours JonJ and 1@ hours JoffJlight cycle. *ights are on from ?am to 9pm." ;f the lights are not going off when they should' this can alter theircircadian rhythm.11. Kou might also want to consider the age of your mice / generally mice will breed until approimately months. ;f breeders do not deliver after 6 / 7 wee%s' try a different male or female. ;n particular' C?9#*6Gmales tend to be la,y.12. 5ry changing diets. 4ietary fat can play an important role in mouse health and breeding performance. ;f youare feeding a fat try raising it to a 6 fat or try lowering from a 6 to a . At 5he Gac%son *aboratory' wemaintain the majority of our breeding colonies on a 6 fat diet *ab4ietL ?F?2". $ome strains e.g. 4#A1'4#A2"' however' breed more successfully on a lower " fat diet. Other institutions maintain their breeders ona 1@/12 fat Jbreeder chowJ diets. $ome strains' however' e.g. C?9#*6" may become obese on higher fatdiets' which' ultimately' may diminish their breeding performance.


RODENT "EANING

=ice

staff no longer performs weaning

=ales must be separated from females at 21/27 days to avoid overcrowding and inbreeding eceptions

are when mice appear underdeveloped"

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;t is recommended by the to house no more that mice per cage and at least two mice housed

together if possible

An ;nternal Animal Ac0uisition &orm must be completed and turned into the staff for any surviving

mice

;f mice must be weaned by staff then a M2?.@@ charge per hour incurs with a minimum charge of 3@

minutes

(lease read following memo regarding more on current charges

!ats

(lease follow same procedure as mice ecept as rats grow in si,e they must be separated into pairs to

avoid overcrowding


MANAGEMENT O# BREDING COLONIES

VMU Res+onsii,i)*

5he will notify investigators of pregnant mice' when to wean' and separate overcrowded

cages.

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Rese%r&- In.es)i%)ors Res+onsii,i)*

5he research investigator is responsible for separating pregnant mice' weaning pups' and

separating overcrowded cages at the appropriate times.

No)ii&%)ion Ti1e Li1i)s

hen the research investigator is notified of to wean pups they will have 1 wee% to wean pups.

hen the research investigator is notified to wean pups and the mother is pregnant with a 2 nd litter

then the research investigator must wean the pups from the 1 stlitter at 21 days.

hen the research investigator is notified of an overcrowded cage then the research investigator will

have 7 hours to separate mice.

hen a research investigator is notified of a pregnant mouse and other mouse are present then the

research investigator has 7 hours to separate the other mice from the pregnant mouse. An eceptionto this would be one male mouse left in with the female mouse so long as the research investigator isdiligent about weaning on the 21stday to avoid birth of new pups mied with older pups.

#%i,re )o &o1+,*

;f the research investigator fails to separate pregnant mice' wean the pups' and not separate

overcrowded cages at the appropriate times it becomes an animal welfare issue and must be reportedto the Animal Committee' O!O' and AAA*AC for non/compliance.

;n the event the staff has to separate pregnant mice' wean pups' and separate overcrowdedcages the research investigators will be charged a special service fee of M2? per hour with a minimumcharge of 3@ minutes of the staff+s time.

5he research investigator is only allowed three failures to separate pregnant mice' wean pups' andor

separate overcrowded cages during the 3 years of the active protocol. ;f the research investigatoraccumulates 3 failures during the 3 years of the active protocol the will report the researchinvestigator to the Animal Committee' O!O' and AAA*AC for non/compliance to animal welfare.

STANDARD OPERATING PROCEDURE#ASTING ANIMALS

(lease label all cages that are to be fasted with a red notification card supplied by the staff. 5he

notification card should be completely filled out with (rotocol number' date and time of fasting' phonenumber of person responsible for fasting animals' and a signature.

5here are fasting rat cages located in the storage rooms of the . (lease as% the staff to hoo%

up cages to the automatic watering system

5here are a total of 12 mice metabolic cages. $i cages are set up and located in the clean side of room

#27. 5hese are available for all investigators and please notify the staff in advance if more cages

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are needed. (lease ma%e sure the metabolic cages are included on the approved protocol before theanimals are placed in the metabolic cage.


HOUSING DIMENSIONS

5he following is 0uoted from the Duide for the Care and >se of *aboratory Animals:

henever it is appropriate' social animals should be housed in pairs or groups' rather thanindividually' provided that such housing is not contraindicated by the protocol in 0uestion anddoes not pose an undue ris% to the animals #rain and #ention 19". 4epending on a variety ofbiologic and behavioral factors' group/housed animals might need less or more total space peranimal than individually housed animals. !ecommendations provided below are based on theassumption that pair or group housing is generally preferable to single housing' even whenmembers of the pair or group have slightly less spaceper animalthan when singly caged.

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$tandardCage

AutomaticateringCage

N1@g 11 mice 12 mice

1@/1?g 7 mice mice

1?/2?g ? mice 6 mice

2?g mice ? mice

up to 1?@g rats

1?@/2@@g 3 rats

2@@g 2 rats

#,oor Are% or C%es$tandard mouse cages P 11in by 6in P 66 s0. in.Automatic watering mouse cages P 11in by 9in P 99 s0. in.!at cages P 16.? by 7.? P 1@ s0. in.

Per Die1 C%e C-%res (As o M%* 2003!

Animal -on


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STANDARD OPERATING PROCEDUREAUTOMATIC "ATERING RAC4

STANDARD OPERATING PROCEDURETEMPERATURE GUIDELINES

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5he recommended temperature range for the animal rooms is between 678/928. (lease use the followingguidelines when the temperature is out of normal range.

1. Call the emergency etension 391?2. (lace portable air conditionerheater units into animal rooms. 5hese units can be set to maintain the

specified temperature3. ;f further assistance is needed refer to the Emergency Cascade 5elephone *ist


PEST CONTROL GUIDELINES #OR RESEARCH #ACILITY

;-5!O4>C5;O-:

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5he improper use of pesticides in aerosol' li0uid' or powder forms can adversely affect biologicalsystems. !esearch animals are vulnerable to such toic agents and should be protected from anyeposure to such materials. (esticides are capable of interfering with biological assays and many othertypes of eperimental procedures in the laboratory as well.

(O*;CK:

A** (E$5 CO-5!O* EQEC>5E4 ;- 5BE A-;=A* &acility of !esearch will be in compliance with

the Duide for the Care and >se of *aboratory Animals. (est control in individual laboratories will beapproved by the principal investigator of that laboratory.

(!OCE4>!E$:

A. !eport any pest control problem' i.e. ants' roaches' sow bugs' maggots etc. to the AdministrativeOfficer' !esearch 4evelopment at etension ?7@1.

1. 5he problem will be evaluated by the AO and the principal investigator' the supervisor of theanimal facility andor the veterinarian if applicable.

2. #uilding =anagement will be notified of the problem. (est Control personnel will consult with

the Administrative Officer or their designee as to the appropriate action. 5he principalinvestigator or animal facility supervisor will be informed of any options and the content of thematerials to be used.

#. Application of the pesticide will proceed only after the Administrative Officer has given approval.

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STANDARD OPERATING PROCEDURENUDE 5 MICE 6 RATS

!E$5!;C5;O-$

Area is restricted to animal care staff and designated research staff.

-o entry into area if you have entered any other animal area ecept rodent rooms" that day.

C*O5B;-D:

=as%' gown' and clean eam gloves

BEA*5B =O-;5O!;-D:

All animals will be chec%ed daily by husbandry staff

(roblems to be reported to the nit $upervisor

4A;*K =O-;5O!;-D:

&looded cages will be spot changed as soon as discovered All cages will be closed and secure

&ood and water chec%ed daily

CADE C*EA-CBA-DE:

Changed every third day with autoclave bedding

&EE4 $CBE4>*E:

Chec% daily' ad lib.

A5E! $CBE4>*E:

Chec% daily' change every third day

!OO= C*EA-;-D:

&loors swept and mopped daily


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DAY ONE

>pon receiving dogs give shots of canine distemper' adenovirus type 2' parainfluen,a' andparvovirus vaccine.

Dive Albon tablets 2? mglb.

4ip dogs in (aramite one o,. per one gallon of water" until s%in is wet allowing dog to sha%e dry'do not rinse.

orm dogs with (anacur for three days.

DAY T"O

Dive Albon tablets 12.? mglbfor six days

STANDARD OPERATING PROCEDUREENVIRONMENTAL ENRICHMENT

6

E8ERCISE PROGRAM #OR DOGS

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4ogs 3@ %g and over will have eercise periods of not less than 3@ minutes per day by means of wal%ing on a

leash andor let run free in the room to have positive physical contact with other dogs.

4ogs will have positive physical contact with humans through play' grooming' petting' or wal%ing on a leash.

4ogs will be provided with toys to %eep them occupied during the long stretches' evenings' and nights when

humans are not present. $uch as rawhide chews' tugs' and other chewable toys."

!adio will play during day light hours with easy listening music to comfort the dogs.

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STANDARD OPERATING PROCEDUREAUTOCLAVING BEDDING

1. !un food container through cage washer

2. 5a%e clean food container to sterili,er room

3. 5a%e bedding bag to sterili,er room

. Autoclave #edding:

(unch holes in bag

Autoclave at 29@ &. for minutes with a drying cycle of at least 2@ minutes.

?. (lace autoclave bedding in clean bedding container

6. 5a%e container and bedding to nude room

9. $pray out side of bedding container with cavicide/@6 before going in nude room

STANDARD OPERATING PROCEDUREBOTTLE "ASHER OPERATION

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O+er%)ion

1. (ull up blue steam valve2. (ull up yellow drain valve3. $hut door completely. 5urn on power switch?. (ress green washer button

6. !eturn valves to original position

Des&ri+)ion#lue valve steam"

located behind machine

handle faces doorway in O&& position parallel to the wall

turn handle ? degrees up to turn O-

Kellow valve drain"

located in front of machine

handle faces wall in the O&& position parallel to the wall

turn handle @ degrees up to turn O-

STANDARD OPERATING PROCEDUREEUTHANASIA

(erforming euthanasia is not the responsibility of the unless the animal is suffering and theinvestigator is not available. 5he veterinarian must be notified prior to euthanasia.

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5urn valve to 1@@@ ($;

5urn second valve to 1@ ($;

RRCo2should be delivered slowly to the chamber to avoid ecitation and stress in the rodent to be

euthani,ed 5he following information was ta%en from Citiprogram.org regarding proper use of carbon dioide

in performing euthanasia and it is also the recommendation of the to follow the A


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Lo&%)ion or Non


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4uring lunch hours see (olice dispatcher at reception des% located in the Emergency !oom of

building 126.

All visitors' contractors' and vendors must have a temporary identification badge which must be

visible and worn above the waist.

All visitors' contractors' and vendors must sign in when arriving and sign out when leaving.

All visitors' contractors' and vendors must have an escort with them at all times.


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!esearch and 4evelopment$tandard Operating (rocedure

$ne 200?

nit 4isaster (lan

PURPOSE 5his $O( describes the steps to be ta%en in case of disasters including earth0ua%es' unusualweather' fire and or other unanticipated events which may disturb the healthsafety of nit" personnel or animals housed in the .

2 POLICY ;n order of priority' the following general rules apply to disaster situations:

a. Personne, s-o,9 no) %&) in >%*s )-%) en9%ner )-eir -e%,)- %n9 s%e)* 5his may includeleaving the area and remaining at a safe location

E,,en 4os1%n(?@2! 0,e(7! 222


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I )-e -e%,)- %n9 s%e)* o )-e +ersonne, %re no) en9%nere9 +ersonne, s-o,9 %&) in >%*s )ose&re )-e -e%,)- %n9 s%e)* o )-e %ni1%,s

c. Personne, s-o,9 no)i* )-e %++ro+ri%)e +ersons of the disaster and the condition of thepersonnel' animals' and building.

DE#INITIONS -one.

RESPONSIBILITIES

;t is the responsibility of the i)- )-e %ni1%,s %) %,, )i1es (i i) is s%e )o 9o so! Contact the

d. Gr%.e,* InFre9 Ani1%,s;n cases in which animals cannot be saved' animals are to beeuthani,ed. !odents will be euthani,ed with CO2according to the $O( for CO2euthanasia. Allother species will be euthani,ed with (entobarbital $odium Euthanasia $olution&atal (lus" 12@mg%g ;< or ;(".

e. Te1+er%)re &on)ro, %n9 .en)i,%)ion ;f power is unavailable for temperature control andventilation for more than a few hours or if room temperatures get too hot or too cold:

i. (rop doors open to allow at least some air echange.

ii. ;ce may be piled on the floors to help cool the rooms.

iii. Animals may be moved out to the hallways for better ventilation if i. and ii. areinsufficient. !odent cages should be covered with filter bonnets when they are ta%en outof their rooms."

iv. 4ogs may be cooled by hosing them down.

f. S-e,)er ;n the event that ade0uate shelter is no longer available at one campus for some or all ofthe animals' they will be moved to the other campus on a space available basis following thepriority set up in SevacuationT above.

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g. Ro9en) &%es Chec% rodent cages and change cages that are very soiled and a threat to thehealth of the animals. 5his includes transgenic mice in sterile caging' even if the hoods are notwor%ing.

h. I %&&ess is res)ri&)e9 or ,oner )-%n >ee'' the animals should be removed to another facilityor euthani,ed to prevent starvation' dehydration' or animal suffering.

i. On&e +-ones s)%r) >or'in %%in5he highest ran%ing person available should contact thefollowing agencies and tell them what has happened.

i. >$4A estern !egional Office: 9@" /997.

ii. O*A Office of *aboratory Animal elfare at -;B": 3@1" 6/9163.

iii. nopened bottled water should be %ept no more than two years.

#oo9 5he maintains a minimum of a one wee% supply of animal feeds at all times. 5his supply will bedispensed to the animals as conditions warrant.

E)-%n%si% 4i)s Each campus will maintain a euthanasia %it containing sufficient syringes' needles' and(entobarbital $odium Euthanasia $olution" to euthani,e all the non/rodent animals at both campuses.

Le%.e C%e R%&' "-ee,s Un,o&'e9: #ecause of the strong jolt and the rolling wave motion that occurs duringan earth0ua%e it is better not to loc% the cage rac% wheels. ;f the rac% is left to roll it shifts with the floor and theanimal cages will remain in place.

#i,)er onne)sFeep a store of emergency filter bonnets to cover cages in transit' along with gloves.

;%r)er,* re.ie> o e1eren&* ,is) %n9 s++,ies5his must be done to be sure supplies are ade0uate and up todate' and that the emergency phone list is up to date. Chec% the following:

a. Emergency water supply At least a three/day supply. 5he water must not be more than two years old".b. Euthanasia %its chec% for ade0uate supplies and that euthanasia solution has not epired".c. &ood supply at least a one wee% supply of food".d. $upply of filter bonnets' gloves' etc. ade0uate supplies"e. Chec% that emergency numbers and staff home numbers are current.

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vmu policy sop manual v-2009-07

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