virginia igem workshop #3 high school education series

Virginia iGEMWorkshop #3High School Education Series

Workshop Plan


• Lesson: Restriction Enzyme Digest, Ligation Reaction, & Gibson Assembly

• Activity: Make Your Own BioBrick (Exploring the Parts Registry)

• Follow-up Reading: Enzymatic Assembly of DNA Molecules up to Several Hundred Kilobases, Gibson et. al.

Restriction Enzyme Digest


Restriction Enzyme Digest: http://igem.org/Videos/Restriction_Digest

Calf Intestinal Alkaline Phosphatase


Ligation Reaction


Ligation Reacion: http://igem.org/Videos/Ligating_Your_Parts

Gibson Assembly


Activity: Make Your Own BioBrick


Follow-up Reading:

Enzymatic Assembly of DNA Molecules up to Several Hundred Kilobases, Gibson et. al.

Virginia iGEMWorkshop #3

High School Education Series

Any Questions?


virginia igem workshop #3 high school education series

Documents

biobrickvirginia igemworkshop

gibson et

gibson assemblyactivity

parts registryfollow