vinamilk pdf delvotest.pdf
TRANSCRIPT
Detection of Antibiotic Residues inVinamilk
Kris FergusonRegional Sales Manager Food and Crop Protection APAC
1Page
Why Test?
1.Example of a European milk qualitycontrol program
2.Problem of fast vs broad spectrumtests
3.Delvotest BLF how to use
4.Best practice when using Delvotest
Content
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Milk Residue Control : Example of the EU
Animal’s milk1-40 Liters
Farm’s tank>>1,000 Liters Truck
>>10,000 LitersSilo>>100,000 Liters
Consumer’s productsYoghurt, Cheese, etc
Dairy Factory
FARM
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DAIRYCONTROL
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Broad Spectrum test.Tests such as Delvotest SP NTor Delvotest T are capable ofdetecting all antibiotics in themilk in 2h30min to 3hours.
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Fast tests for -lactams.Tests such as Delvotest BLFare capable of detecting allantibiotics from the -lactamfamily in 5 minutes.
CONTROL
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Milk Residue Control : Example of the EU
Animal’s milk1-40 Liters
Farm’s tank>>1000 Liters Truck
>>10,000 LitersSilo>>100,000 Liters
Consumer’s productsYogurt, Cheese, etc
Dairy Factory
Laboratory>1,000 samples/day
Good milk quality starts at the farm. Farmers are responsiblefor the delivery of good milk (price increases with quality).
FARM
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DAIRYCONTROL
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Tank sample is takenby the truck andsent to the lab
Farms will befined ifantibioticsare detected.
Strong collaborationbetween Lab and Dairy.The Dairy organises andpays for the testing of themilk samples.
Fast test and broad spectrum testsThe problem
Broad Spectrum test.Tests such as Delvotest SP NTor Delvotest T are capable ofdetecting all antibiotics inmilk in 2h30min to 3hours.
CONTROL
STOP
CONTROL
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Fast tests for -lactams.Tests such as Delvotest BLFare capable of detecting allantibiotics from the -lactamfamily in 5 minutes.
Based on Bacillus bacteriaSensitivity depends on the way
antibiotics acts on the Bacillus.
Based on affinity of antibody or ingeneral proteins with the antibiotic
Sensitivity depends on theselectivity of the binding moleculeto the antibiotics.
Those are different targets withdifferent affinity for antibiotics
different sensitivity profiles
Delvotest BLF
IncubatorUse
Press Program to change timer:“T1 2” means timer 1 : 2minPress on Time Set x times to increase T1 ofx min.When time reached, long press on Time Setwill save the new time.
Press Program again to change timer 2:Same procedure as for T1.
Temperature set (63°C – 65°C):Press “Program” together with “Time Set”to increase temperature.Press “Program” together with “Start/Stop”to decrease temperature.
Press Program to change mode: Auto(Autoamtic switch off – no sound) or Snd(sound is played after incubation)Press on Program to select desired modeand long press on Program to save theselected program.
Time SetPress several times on Time Set to selectthe desired incubation time. (Time loops1:45 to 3:15)
Temperature set (63°C – 65°C):Press “Program” together with “Time Set”to increase temperature.Press “Program” together with “Start/Stop”to decrease temperature.Start /Stop
Power on: press I/O
Display: Display “PrE” “HEAt”“brd”
Display “64°C”and “cont”
T°=64°CStart count downDisplay remaining time
Display “64°C”and “Count”
T°=64°C
Start /Stop
Start count down (2min)Display remaining time
Time elapsed
Bip sound is played“StRp” is displayed
Start /Stop to start 3 min
Start count down (3min)Display remaining time
Time elapsed
Play Bip soundDisplay “REAd”
Start /Stop
Start
Stop5sec
Start
Stop5sec
Display: Display “PrE” “HEAt”“FASt”
Press”I /O” and “Program”+ “I/O” toswitch from broad spectrum test mode
to fast test mode.
Start
Stop5sec
Start /Stop to stop bip soundPlay Bip sound*Display “Read”
AUTO Stop Heating**
Time elapsed
Start/Stop
Delvotest Step by Step
Take representative sample
Cut of or remove ampoulesfrom tray (!DO NOTDAMAGE OTHER PLATE’S orAMPOULE’S FOIL, ifdamaged, will be falsepositive!)
Make a hole (Not necessaryto remove foil forampoules)
Pipette milk (the correct volumeof milk is the one in the pipettetube, not its tank)
Add the milk to the testThe antibiotics is any willdiffuse in the agar gel
Incubate: the bacteriain the agar gel willgrow if no antibioticsin the milk
Read the test. POS or NEGdepending on colors(POS=Not good milk,NEG=Good milk)
Principle of Delvotest
Penicillin 4
Antibiotic freePOSITIVE RESULT
NEGATIVE RESULT
Sulfadiazine 100
Control TimeTime
Colo
r
Control time versus fix reading time
Penicillin 4
Antibiotic freePOSITIVE RESULT
NEGATIVE RESULT
Sulfadiazine 100
Control TimeTime
Colo
r
Fix timeFix time Test less sensitive than at control timeIf incubation time increases, the test sensitivity decreases
Delvotest Colors
Wrong color reading Wrong results!
? ? ?
Delvotest Colorcards for improved reading
Systematically in each box of Delvotest T
On request
On request
On request
Delvotest: Storage and Sampling
Good practice in storage/handlingThe test is sensitive to LIGHT.
Store in the DARK (in original packaging).
The test is sensitive to TEMPERATURE.Store 4-8°C (in original packaging).Place ampoules in fridge directly after cutting out the
required number.
Test should not freeze even partially. (creates milk runnersand/or false positive, test can also become liquid)
Up to 4 days, test can be transported at max 30°C.
The test is sensitive to AIR.Test where foil is removed can dry. (False positive
/ dried test)
What if not stored correctly?Spores DIE (when freezing, agar gel looses structure)Test becomes slower and more sensitiveFalse positives
Thank You!