February 2008 1

BioBiosafety at experimental safety at experimental beamlinesbeamlines

Véronique MAYEUXESRF and ILL Biosafety Officer

February 2008 2

Badges must be worn

visibly

at

all times

on site.

The badges are of the proximity type.To open a door, present your badge within a few cm of the reader until a green light comes on and you hear the door unlocking. Then, turn the handle.

Controlled access

to certain buildings

User card no. 33User card no. 33

February 2008 3

It is strictly forbidden to smoke in all ESRF buildings.

No smoking

Meals

Meals are normally taken at the restaurant or in the coffee areas. However, eating and drinking in beamline control hutches is tolerated.

Alcoholic drinks are strictly forbidden at the work place, in particular in the Experimental Hall.

It is strictly forbidden to eat and drink in beamline lead hutches, in laboratories and in workshops.

February 2008 4

The French highway code is applicable on all the site. Respect it, in particular:

Driving on the site

Respect the 30 km/h speed limit on the site.

Respect the direction of traffic on the roundabout. This also concerns cyclists!

Park only in authorised parking spaces.

Stop at stop signs.

February 2008 5

If you witness to the start of a fire:

◊

AlertIndicate the location and the importance of the fire.Do not hang up first.

◊

InterveneIf it is a small fire use a fire extinguisher.Close the doors and windows.Switch off the electrical supply.

◊

EvacuateIf the fire is too big, evacuate once you have given the alert.If you must cross a smoke-filled zone, walk or crawl as close as possible to the ground.Go to the nearest gathering point.

Emergency procedure: fire

10or

February 2008 6

◊

Listen attentively to the message.

◊

Stay calm.

◊

Evacuate the building (without using the lifts).

◊

Make your way to a gathering point.

◊

Wait for further instructions.

◊

Do not re-enter the building without have received the authorisation to do so.

Emergency procedure: evacuation of a building

Siren + message

In case of the evacuation of a building, a siren, followed by a message in English and in French, will be broadcasted continuously over the loudspeaker system.

February 2008 7

Emergency procedure: evacuation of a building

The gathering points are identified by large red signs with four

white arrows. There are a total of 6 gathering points on the site, each corresponding to a

part of the buildings as indicated on the map below.

February 2008 8

Emergency procedure: shelter in place

◊

Listen attentively to the message.◊

Leave your work in a safe condition.◊

Close the doors and windows.◊

If you see someone outside the buildings, request them to come in.◊

Do not use the lifts or the telephones.◊

Take shelter, respecting the procedures, described in the following page.

◊

Wait for further instructions.

Siren + message

In case of shelter in place, a siren, followed by a message in English and in French, will be broadcasted over the

loudspeaker system.

Shelter in place may be decided in case of an accident taking place outside the site and which might create a risk for the health of

persons on site, for example following an accident on the

motorway involving a truck carrying dangerous products.

February 2008 9

During confinement, persons present in the buildings indicated in blue on the map, including the Experimental Hall, must take shelter in the Central Building. Persons present on the long beamlines ID17 and ID19 take shelter there.Persons present in the three Guest House buildings may take shelter in either of the buildings. Persons present in the staff restaurant take shelter in the building. Persons present in the

Cafeteria must immediately make their way to the staff restaurant.

These instruction are posted in all the buildings near the emergency exits.

Emergency procedure: shelter in place

February 2008 10

◊

The evacuation will be by car or by foot depending on the urgency of the situation.

◊

During an evacuation by foot, all staff working at the ESRF must

leave the site by the emergency exit gate leading on to avenue des Martyrs.

◊

The total evacuation of the site by car takes place according to

the procedure outlined in the following pages.

Emergency procedure: total site evacuation

Siren + message

In case of a total evacuation of the site, a siren, followed by a message in English and in French, will be broadcasted continuously over the loudspeaker system.

An evacuation of the site may be decided in case of an serious accident taking place outside the ESRF site.

February 2008 11

Emergency procedure: evacuation by car

The total evacuation of the site by car takes place via the emergency exit gate, following the evacuation itinerary indicated in green on the map below. Persons located in the areas marked in red on the map (including the Guest House), as well as all persons without a vehicle, must make their way to the special gathering point near the Central Building, where they will be picked up by persons with cars.

Gathering point for persons without vehicles

The signs indicating the direction to follow.

Emergency exit gate

February 2008 12Watch out for pedestrians walking in the Hall.

Traffic on site: bicycles in the Experimental Hall

ESRF bicycles:

Experimental Hall

Technical Gallery

Outside

Watch out for forklifts in the Hall.

Watch your speed.Keep both hands on the handlebars.

February 2008 13

If you bring electrical equipment with you which is not certified CE conform, you must contact the Hall Operators (tel: 25 25) before operating this equipment.

You are not authorised to intervene on electrical equipment. In

case of a problem, contact the Hall Operators.

Electrical safety

Magnetic fields

Due to the presence of magnetic fields in the Experimental Hall,

persons wearing pacemakers must contact their Works Doctor for advice.

February 2008 14

Specific risks: radiation protection

Sources of ionising radiation at the ESRF:

AcceleratorsBeamlinesX-ray generatorsRadioactive sourcesRadioactive samplesInduced radioactivity

All people working at the ESRF (ESRF and CRG staff, users, external companies) are considered as non-exposed workers.

Annual limit for the public: 1 mSv/year(or 0.5 mSv/h, assuming 2000 working hours per year).

February 2008 15

Specific risks: radiation protection

While the shielding and the interlocked radiation monitors guarantee that the radiation levels outside the accelerator tunnels and beamline hutches are sufficiently small, the

Personnel

Safety System (PSS) must guarantee that nobody

can be present inside an area where there is a potential radiation hazard. All areas concerned are indicated with appropriate safety signs.

February 2008 16

Lasers

Magnetic fields

Electro-magnetsand permanent magnets

Danger for people with pacemakersNo access

! Other risks

February 2008 17

WhatWhat to do in case of an emergency to do in case of an emergency withwith

a a biologicalbiological agentagent

StayStay calmcalm and do not panicand do not panic

* In case of * In case of cutcut or or puncturepuncture, , immediatelyimmediately washwash cautiouslycautiously the the woundwound withwith taptap water and soap for water and soap for atat least 5 least 5 minsmins..

* In case of * In case of eyeeye projectionprojection, , immediatelyimmediately rinserinse youryour eyeseyes widelywidely open open withwith the the eyeeye--fountainfountain for for atat least 20 least 20 minsmins and go to and go to seesee an an ophtalmologistophtalmologist..

ThenThen go the go the thethe medicalmedical service for service for furtherfurther care and care and informinform the the biosafetybiosafety officerofficer..

LaterLater on, on, youyou willwill bebe askedasked to to answeranswer few questions about the few questions about the circumstancescircumstances of the accident of the accident and the nature of the and the nature of the biologicalbiological agent agent involvedinvolved..

February 2008 18

What to do in case of an accident

http://intranet.esrf.fr/Directorate/Safety/EmergencyPlan/SafetyRules

small injury

first aid kits(secretaries’

offices, beamlines)

or

Medical Service8h –

12h15 & 13h15 –

17h on working days

Accidents must be entered in the register for minor accidents, whatever the seriousness of the illness of the injury.

more serious

call for help

Intervention of Medical Service / outside assistance

Accidents must be declared to the Social Security within 48hrs. The form is available from the Personnel Service.

101010

3333

February 2008 19

Emergency Emergency proceduresprocedures BeforeBefore

youyou

startstart, Do know the , Do know the safetysafety

signssigns……

Emergency exits and evacuation routes

o Fire extinguishers

o Spill kits and First aid kits

o Emergency showers

o Eye-wash fountains

Know Know youryour nearestnearest first first aidersaiders

Emergency : dial # 10 (ESRF control room)Emergency : dial # 10 (ESRF control room)

February 2008 20

Experimental stationsExperimental stations and and samplesample

labslabs

The Local ContactThe Local Contact

AssistsAssists in the use of the in the use of the experimentalexperimental station.station.ChecksChecks UsersUsers’’safetysafety training training donedone on the ESRF web page.on the ESRF web page.InformsInforms, (, (YELLOWYELLOW or or RED RED SAF) the SAF) the specialspecialrequirementsrequirements for the for the experimentalexperimental session session withwith help of the help of the SafetySafety..InformsInforms the the SafetySafety group or the group or the ExperimentalExperimental Hall Hall OperatorOperator (EHO) about (EHO) about anyany accident or incident. accident or incident. ChecksChecks the the experimentalexperimental station (station (includingincluding the the samplesamplepreparationpreparation lablab) ) beforebefore and and afterafter eacheach experimentalexperimentalsession.session.

February 2008 21

Safety kits/DisinfectantsSafety kits/DisinfectantsSafety kitSafety kit (Goggles, Eye-wash, Absorbent, Disinfectant, sometimes mask)

Phagosept SprayPhagosept SprayDisinfection of equipment (e.g. Disinfection of equipment (e.g. microscope, bench top)microscope, bench top)

Stellisept ScrubStellisept ScrubHand-washing after work and when contamination is suspected.

KorsolexKorsolex--plusplusDecontaminant (in case of accident ONLY!)

February 2008 22

Biological SafetyBiological Safety Biological agentsBiological agents

Any agent which may cause any infection, allergy, toxicity or caAny agent which may cause any infection, allergy, toxicity or can create n create any hazard to human health and/or environmentany hazard to human health and/or environment

MicroMicro--organismsorganisms such assuch asFungi (including yeasts), Fungi (including yeasts), BacteriasBacterias, Viruses, , Viruses, PrionsPrions

ParasitesParasitesGenetically modified organisms (Genetically modified organisms (GMOGMO’’ss))Cell culturesCell cultures such assuch as

Mammalian cell cultures (animal and human)Mammalian cell cultures (animal and human)Plant cell culturesPlant cell cultures

Biological risk

February 2008 23

BiologicalBiological safetysafety

HazardHazard groups (groups (sincesince

1994)1994)

Hazard group Pathogenicity for humans

Hazard to workers

Spread to the community

Effective prophylaxis or treatment

1Unlikely to cause human disease

Unlikely Unlikely Available

2Can cause human disease

Maybe Unlikely Usually available

3Can cause severe human disease

Maybe serious May spread Usually available

4Causes human disease

Serious Likely Usually non

Adult healthy

worker

!!!

February 2008 24

Biosafety/BiosecurityBiosafety/Biosecurity DeclarationDeclaration

of of controlledcontrolled

biologicalbiological

agentsagents

AFSSAPSAFSSAPS : Agence Fran: Agence Franççaise de Saise de Séécuritcuritéé Sanitaire des Produits de Sanitaire des Produits de SantSantéé

ArrêtArrêtéé du 30 juillet 2004du 30 juillet 2004This This decreedecree takestakes intointo the the accountaccount the notion the notion ““all or part ofall or part of”” ((tout tout ou partieou partie) of the ) of the biologicalbiological agents, agents, whichwhich meansmeans thatthat all all genesgenesand and theirtheir productsproducts (i.e. recombinant (i.e. recombinant proteinsproteins) ) fallfall underunder the the control of the AFSSAPS control of the AFSSAPS

It It concernsconcerns importation, exportation, possession, importation, exportation, possession, transfertransfer, use, use

DownloadsDownloads and and explanationsexplanations are on the web page. are on the web page. RequestRequest willwill taketake atat least least threethree weeksweeks

February 2008 25

BiosafetyBiosafety//BiosecurityBiosecurity Importation of Importation of humanhuman

samplessamples

HumanHuman samplessamples likelike organsorgans, tissues, , tissues, fluidsfluids, , ……

Importation Importation fromfrom outsideoutside of France :of France :DeclarationDeclaration to to bebe made made atat the the MinistryMinistry of of ResearchResearch (4 (4 registeredregistered copies)copies)

DoesDoes not not applyapply for for purifiedpurified proteinsproteins, , hairhair, , nailsnails

DownloadsDownloads and and explanationsexplanations are on the web page. are on the web page. RequestRequest willwill taketake atat least least twotwo monthsmonths!!!!!!

February 2008 26

Contamination routesContamination routesTransmission Practices

Respiratory Aérosols While pipeting, centrifuge, sonication, filtration, Culture dish opening, lyophilisated products

CutaneousIrritated or

Damaged skinPuncture, wound, bite or scratch from animals, damaged skin or irritated

Digestive swallow Mouth pipeting, cigarette, meals taken in labs

Conjonctive Projection, contactEye Projection : culture drops, infectant aerosolsContaminated tools or machines in contact with eye lids or eyes, contaminated gloves or hands

February 2008 27

Evaluation of an experimentEvaluation of an experiment SampleSample

sheetssheets

* Sample name (* Sample name (or functionor function))* Source origin * Source origin (organism + strain)(organism + strain)

* Classification * Classification (risks you know)(risks you know)

* Question about possible * Question about possible dangers dangers (The ones you are aware of)(The ones you are aware of)

* Manipulation at the ESRF and * Manipulation at the ESRF and Request for Lab facilitiesRequest for Lab facilities(sample mounting or more processing to (sample mounting or more processing to be done)be done)

February 2008 28

Evaluation of an experimentEvaluation of an experiment SafetySafety

ApprovalApproval

FormForm

(SAF)(SAF)

GREEN GREEN -- no biological riskno biological risk

YELLOWYELLOW -- precautionsprecautionsOpen questionsOpen questionsGood laboratory practiceGood laboratory practiceSamples falling under control of authoritiesSamples falling under control of authorities

REDRED -- biological riskbiological riskStrict protocol to be followedStrict protocol to be followedExperiments start only after checked by the Experiments start only after checked by the

Safety group, Presence 24h/24h with the Safety group, Presence 24h/24h with the sample or locked up sample or locked up

No red experiments on weekNo red experiments on week--endsendsNo automatic mode for sample changer.No automatic mode for sample changer.

Safety Approval

Form

February 2008 29

Biological agents: bits and piecesBiological agents: bits and pieces BeforeBefore

youyou

startstart

youryour

experimentexperiment……

InvolvingInvolving proteinprotein crystalscrystals……SampleSample sheetsheet: : properproper classification of classification of youryour source source originoriginFollowFollow the the protocolsprotocols for for yellowyellow and and redred experimentsexperiments..Be Be carefulcareful whenwhen handlinghandling heavyheavy atomatom solutions.solutions.MakeMake sure the sure the safetysafety kit kit isis completecomplete..For For usingusing laserslasers……....((specsspecs))LeaveLeave the the workwork area area clean and clean and tidytidy afterafter use. use. ((wasteswastes))

InvolvingInvolving humanhuman cellcell lineslines, tissues, tissues……For manipulation : For manipulation : workwork in in appropriatelyappropriately designeddesigned laboratorylaboratory((containmentcontainment levellevel L1/L2).L1/L2).Requires Requires BiosafetyBiosafety training (Lab use and sample handling)training (Lab use and sample handling)Close Close medicalmedical followfollow--up (e.g. vaccinations) up (e.g. vaccinations) mightmight bebe necessarynecessary

February 2008 30

ChemicalChemical risksrisks

How do I protect myself and the environment ?How do I protect myself and the environment ?

Know your protocol.Know your protocol.Know your chemicals and look at the material Know your chemicals and look at the material safety data sheet (MSDS) in your lab or on the safety data sheet (MSDS) in your lab or on the web.web.Use small quantities of chemicals and try to Use small quantities of chemicals and try to consider safer substitutes or protocols when consider safer substitutes or protocols when possible.possible.Wear safety glasses, proper gloves, and a lab Wear safety glasses, proper gloves, and a lab coat when handling hazardous chemicals.coat when handling hazardous chemicals.Know the disposal of your chemical waste.Know the disposal of your chemical waste.

February 2008 31

Liquid nitrogenLiquid nitrogenLiquid nitrogen gives severe skin burns

Safety goggles and appropriate gloves should be worn when handling liquid nitrogen

Goggles and Gloves are marked and dedicated to one specific BL.Goggles and Gloves are marked and dedicated to one specific BL.

Must be transported in appropriate Dewar's

Do not travel with gas cylinders or liquid gases in the lift or do not work in closed rooms which are not sufficiently ventilated

1 litre of liquid nitrogen generates 646 l of gas

1 litre of helium gas generates 700 l of gas

Warning with dry ice (Carboglace). Bigger quantities should be stored outside the building.

Chemical risk

February 2008 32

Protection Protection EquEquipment (PE)ipment (PE) Laboratory chemical hoodLaboratory chemical hood

Place everything you need in the Place everything you need in the hood before starting and switch it on hood before starting and switch it on in advance.in advance.Lower the sashLower the sash.. Always make sure Always make sure the sash is pulled down to the the sash is pulled down to the marked level. The best protection is marked level. The best protection is provided when the sash is brought to provided when the sash is brought to the lowest level for convenient the lowest level for convenient operation.operation.Use Use slow and deliberate motionsslow and deliberate motions. . Place supplies so that they do not Place supplies so that they do not obstruct the airflow. obstruct the airflow. Clean the Clean the hoodhoodbeforebefore and and afterafter use !!!use !!!Avoid storing hazardous chemicals Avoid storing hazardous chemicals inside the hood it is inside the hood it is not a storage not a storage areaarea..

February 2008 33

Genotoxic compounds

Carcinogens Mutagens Teratogens

Causes cancer affect any cells or tissues

Hereditary genetic defectsEffect germ cells (gonads)

Non-hereditary congenitalmalformations

Effect growing fœtus

Arsenic acid and salts( cacodylic buffers )

AcrylamideBeryllium

Cadmium saltsCobalt and Nickel salts

Crystal violet….

PhenolToluene

Mercury compoundsLead compounds

CadmiumDMSO

FormaldehydeEstradiol

…..

Ethidium bromideAcrylamide1,3-butadien

Potassium chromate and dichromateCadmium salts

….

February 2008 34

Category 1 and Category 2

Risk statements labelR 45 : can cause cancerR 49 : Can cause Cancer

by respiratory tractR 46 : Can cause hereditary

genetic alterationsR 60 / 61 : Can alter fertility / Risks duringpregnancy, hazardous effects for the child

Category 3

Risk statements labelR 40 : Carcinogenic effects suspected

unsufficient proofsR 68 : Potential irreversible effects

R 62 / R 63 : Possible risks of fertility alterations / Possible risks during pregnancy, hazardous effects for the child

GenotoxicGenotoxic compoundscompounds

GeneralGeneral or or specificspecific

toxictoxic

effectseffects

cancan

occuroccur

quicklyquickly

((especiallyespecially

on reproductive on reproductive germgerm

cellscells

in in humanshumans).).HoweverHowever, , otherother

effectseffects

are are notnot

revealedrevealed

beforebefore

yearsyears

afterafter

exposureexposure..((protectiveprotective

measuresmeasures

to to preventprevent

anyany

trouble are trouble are missedmissed

or or ignoredignored).).

How to recognise them ?

Chemical risk

Read the MSDS !!!

February 2008 35

Office WastesOffice Wastes

Normal solid waste only in control cabin

and offices

Paper to be recycled only in control cabin

and offices

Wastes

February 2008 36

Infectious biological wastesInfectious biological wastes from Containment Level 1 from Containment Level 1

Solid biological waste to be autoclaved

Needles and sharps objectsSolid biological

waste to be incinerated

Wastes

February 2008 37

Other Lab waste Other Lab waste containerscontainers Please label according to the chemical to be disposed offPlease label according to the chemical to be disposed off

Acrylamide, Ethidium bromide, Sybr stain, solutions in falcon or eppendorf tubes

For broken glass as slides, capillaries, others and heavy

metals

Wastes

February 2008 38

Chemical WasteChemical Waste Please label according to the chemical to be Please label according to the chemical to be

disposed offdisposed off

Liquid chemical wastes :•Acids•Bases

•Halogenated solvents•Non-halogenated

solvents• label according to toxic

waste

Wastes

February 2008 39

Transport of biological samplesTransport of biological samples

Document for Airport Security check when leaving the ESRF from Lyon, Grenoble or Geneva Airport

Form to be requested in advance to the Biosafety officer by email (expsaf@esrf.fr and mayeux@esrf.fr) when submitting A-form (Tick box).

Let the Safety group know about any problem during travel with biological samples (expsaf@esrf.fr and mayeux@esrf.fr)

European Synchrotron Radiation Facility Installation Européenne de Rayonnement Synchrotron

ESRF

Grenoble, 02-02-2006 Ceci est une boîte de transport pour des plaques de cristallisation et/ou un récipient d’azote liquide contenant

des échantillons biologiques non dangereux qui ont été utilisés pour des expériences à l’ESRF (European

Synchrotron Radiation Facility) de Grenoble.

Manipulez-les avec précaution.

Ces échantillons sont de minuscules cristaux, de l’ordre du nanomètre, extrèmement fragiles. Ils referment une

toute petite quantité de protéines hautement purifiées. Les plaques de cristallisations contiennent de petites

gouttelettes, de l’ordre de 1 micro-litre à 100 nano-litre, dans lesquelles les protéines cristallisées restent

stables.

Attention : Un changement de température ou un mouvement brusque du récipient d’échantillons pourrait

entraîner leur destruction.

Cet envoi contient, sous forme hautement purifiée, les cristaux des protéines suivantes :

List des echantillons Nom Origine Organisme recepteur ( s’il y a

lieu) ATTENTION :

Ces échantillons ne représentent aucun risque biologique pour l’homme ou l’environnement. En cas de

dispersion accidentelle vous pouvez nettoyer à l’aide d’un chiffon mouillé contenant un détergent classique et rincer à l’eau.

En cas de question ou de problème, vous pouvez contacter :

Sigrid Kozielski – Ingénieur Sécurité Biologique de l’ESRF (04 76 88 22 03)

Paul Berkvens – Responsable du Service Sécurité de l’ESRF (04 76 88 20 71)

Martine Moroni – Ingénieur Sécurité des Expériences de l’ESRF (04 76 88 23 69)

Patrick Schlick – Aéroport de Lyon, Service de la Police aux frontières (04 72 22 74 08)

Bien à vous,

Ingénieur sécurité biologique de l’ESRF Transporteur des echantillons

Name: Sigrid Kozielski

Nom: Laboratoire:

Commentaire: Addresse:

Signature: Signature:

February 2008 40

OnOn--sitesite infos on Biosafetyinfos on Biosafety

ESRF ESRF webpageswebpagesSafetySafety regulationsregulations ((frenchfrench andand englishenglish) on intranet) on intranetPreparationPreparation ofof biologicalbiological experimentsexperiments andand formsforms to to bebe downloadeddownloaded

http://www.esrf.fr/Infrastructure/Safety/Experiments/Biology_Exphttp://www.esrf.fr/Infrastructure/Safety/Experiments/Biology_Experimentseriments

ILL ILL safetysafety webpageswebpagesSafetySafety notes notes availableavailable on ILL intranet on ILL intranet Notes on Notes on biosafetybiosafety andand formsforms to to bebe downloadeddownloaded

http://www.ill.fr/index_sc.htmlhttp://www.ill.fr/index_sc.html

EMBL EMBL webpageswebpagesSafetySafety webpageswebpages @ @ EMBLEMBL--GrenobleGrenobleFromFrom ESRF via ESRF via wwi.emblwwi.embl--grenoble.frgrenoble.frSafetySafety webpageswebpages @ @ EMBLEMBL--HeidelbergHeidelberg ((onlyonly accessible @ EMBL)accessible @ EMBL)

February 2008 41

OnOn--sitesite infos on infos on BiosafetyBiosafety

ESRF ESRF internetinternet webweb pagespages::

SafetySafety regulationsregulations ((French French andand EnglishEnglish))UsersUsers’’trainingtrainingFireFire trainingtrainingPreparationPreparation ofof biologicalbiological experimentsexperiments

http://www.esrf.eu/Infrastructure/Safety/Experiments/Biology_Experiments

Forms to be downloaded for AFSSAPS controlled agents, Human samples and derivatives importation, sample sheets,...

February 2008 42

Exp. Safety contactsExp. Safety contacts

Martine MORONIExperimental Safety EngineerTel: 23-69Office: EXPH S-30 1-05, (moroni@esrf.fr)

Katty

TAXILExperimental Safety TechnicianTel: 21-76Office:

EXPH S-30 1-05, (taxil@esrf.fr)

Véronique

MAYEUX

Joint Site Biology and Biochemistry Safety EngineerTel: 22-03Office: EXPH S-30 1-04, (mayeux@esrf.fr)

February 2008 43

The Hall Operators

The Hall Operators can provide you with

safety or technical assistance 24h/24. You can contact them by telephone by dialling 25 25

or by going directly to their office S30-0-

13 situated in the Experimental Hall opposite ID 32.

If your are working alone on a beamline or in a laboratory, you must contact a Hall Operator to receive a warning device for persons working alone.

If you are unable to start a beamline because the “Operator”

key is missing from the Personnel Safety System (PSS) panel, contact the Hall Operator.

February 2008 44

TTelephone Numberselephone Numbers

Emergencyor

Experimental Hall Operators: 2525

(24h/24h)

Safety Group :2403

10