varian 900-lc series i hplc. 2 what is the i hplc? 2 models 920-lc and 940-lc compact, stylish,...
TRANSCRIPT
![Page 1: Varian 900-LC Series i HPLC. 2 What is the i HPLC? 2 models 920-LC and 940-LC Compact, stylish, automated analytical and semi prep HPLC Pre-plumbed and](https://reader035.vdocuments.site/reader035/viewer/2022062423/56649eb35503460f94bba123/html5/thumbnails/1.jpg)
Varian 900-LC Series iHPLC
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What is the iHPLC?
• 2 models 920-LC and 940-LC
• Compact, stylish, automated analytical and semi prep HPLC
• Pre-plumbed and factory tested for the highest confidence
• Easy to order, install and use
– Single Ethernet cable connection
• Focused on improved productivity through automation
• Updated premium performance hardware
• Autosampler, Fraction Collector and Scale up Module for semi-prep
• Quaternary pump, precision Column Heater,RI, Fluor, ELSD and autosampler for analytical
• Updated Integrated Instrument Software Interface
• Eliminate repetition, modernise UI
• Consistent
• Easy to use with added functionality
• Adds to modular range
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Varian 920-LC Integrated Analytical HPLC
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Varian 920-LC What Is It?
• Compact, stylish automated analytical HPLC
– Pre-plumbed and factory tested for the highest confidence
– Single cable communications
• Virtually pulse-free dual piston pump
– Does not require a pulse damper
• State-of-the-art low pressure quaternary gradient module with built-in degasser
• Autosampler with cooling option
– Synchronised injection for outstanding retention time reproducibility
– Compatible with 200 x 1.6 ml HPLC vials or 3 microplates
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Varian 920-LC What Is It?
• Either dual UV-Vis or PDA detector
– Optional second detector - Fluorescence or RI
• High precision (<0.15oC) dual column heater available
– Low delay solvent pre-heater for improved heat transfer
– Optional column switching and electric injection valve
• NEW improved instrument interface on GalaxieTM software
• External CVM, ELSD or 356-LC RI can be plugged directly into the back of the unit.
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Varian 920-LC Silver Bullets
• Premium analytical pump performance and reliability
• Superb Fast LC performance optimised for sub 3 um columns
– Improved throughput without compromising robustness
• High sensitivity/top line performance detection systems across UV/PDA/RI/Fluor and ELSD – unparalleled strength
• Unbeatable Injection performance and carryover statistics with 2008 loopless technology autosampler
• Full system level computer controlled AIQ (validation)
• Updated driver for enhanced user experience
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920-LC from Conventional to Fast HPLC
25cm x 4.6mm Pursuit XRS 5um 1.0ml/min
25cm x 4.6mm Pursuit XRS 5um 2.2ml/min
15cm x 4.6mm Pursuit XRS 5um 1.0ml/min
15cm x 4.6mm Pursuit XRS 5um 3.75ml/min
10cm x 2.0mm Pursuit XRS 2.8um Ultra 0.8ml/min
5cm x 2.0mm Pursuit XRS 2.8um Ultra 0.4ml/min
5cm x 2.0mm 2.4um 1.3ml/min16 x Speed Enhancement
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Varian 920-LC Fast LC PAHs
Varian 920-LC6 minute Analysis
Waters Acquity7 Minute Analysis
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Varian 920-LC Solvent Delivery System
• Dual piston quaternary pump produces fast gradients – even at 200 l/min
• Virtually pulse-free pumping without pulse damper
• Outstanding gradient compositional accuracy give retention time precision at the <1% level
• Built-in vacuum degas maintains pump prime and enhanced injection reliability
• Accurate flow from 0.1 to 10 mL/min for 2 to 4.6 mm columns
• Overlay of 10 chromatograms
• 10% steps at 1ml/min
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Varian 920-LC Autosampler
• Needle-in-flow path geometry for low carry-over (<0.01%)
• Typical inj precision (< 0.3% at 10 l)
• Optimized flow paths for fast injection turn-around
• Synchronised injection for best RT reproducibility
• Programmable wash setting for low carry-over
• Injection volumes from 1ul to 50L
• Cooled option available
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Varian 920-LC Washout Performance
Blank injection <0.01% carryover
125 ug/ml Caffeine
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Varian 920-LC Injection Synchronization
-20%
0%
20%
40%
60%
80%
100%
120%
0 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 1.8 2 2.2
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Sensitive UV-Vis Detectors
Typical Agilent VWD S/N ~ 25
Typical Varian 920-LC UV/Vis S/N > 36
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Information Rich Diode Array Detection
• 1024 pixels provides full coverage from 190 – 950 nm
• Full spectra collection• Enhanced purity
• Automatic peak identification with customised libraries• Greater confidence on compound identification
• Choice of flowcells• 4 mm path length semi-micro • 9mm path length analytical
• Front panel access• Lamps and flowcells
• D2 and Vis lamps self aligned• Easily user maintained
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Information Rich Diode Array Detection
• Automatic peak identification with customised libraries• Use spectra as well as RT for compound ID
• Greater confidence on compound identification
• Peak search of unknown yields 998.7 match for naphthalene
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Varian 920-LC Fluorescence Detector
• Sensitive Fluorescence detector (S/N > 600 Raman H2O peak baseline method)
• Time programmable for increase sensitivity• Automatic validation with built-in Hg lamp• Low 12 l dead volume flowcell for improved
chromatography
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Varian 920-LC Refractive Index Detector
• Based upon 356-LC RI detector• Low volume, flow efficient cell for superior peak shape• Stable, long life and high intensity LED
• Minimises drift
• Provides excellent detection limits
• Enhanced life-time
• Temperature controlled optics minimises baseline drift and provides outstanding detection limits
• Complete automation thru Galaxie software
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Varian 380-LC and 385-LC ELSD
• Universal HPLC detector• Compatible with gradients
• More sensitive than RI
• NEW Glass Nebuliser for Improved Reproducibility• 2 to 3 Times More Sensitive
• 40Hz Data Rate for Fast LC• Complete Galaxie compatibility
• Program changes thru a run
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Heated Column Compartment
• Temperature range from ambient + 10oC up to 65oC
• Excellent temperature precision +0.15oC
– Ensures retention time reproducibility
– Eliminates re-runs
• Good temperature accuracy <1oC
– Low delay volume pre-heater for efficient heat transfer to mobile phase
• Rapid warm-up
– Typically from ambient to 40oc in <15 minutes
• Spacious - easily accommodates 2 x 250mm columns and 2 valves
• Option with electrically actuated column switching valve and injection valve
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Excellent Long term Stability
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50
11:4
6:00
12:2
2:00
12:5
8:00
13:3
4:00
14:1
0:00
14:4
6:00
15:2
2:00
15:5
8:00
16:3
4:00
17:1
0:00
17:4
6:00
18:2
2:00
18:5
8:00
19:3
4:00
20:1
0:00
20:4
6:00
21:2
2:00
21:5
8:00
22:3
4:00
23:1
0:00
23:4
6:00
0:22
:00
0:58
:00
1:34
:00
2:10
:00
2:46
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3:22
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3:58
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4:34
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5:10
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5:46
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6:22
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6:58
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7:34
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8:10
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8:46
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9:22
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Time
Te
mp
era
ture
(d
eg
ree
s C
)
Mobile phase input Temp
Mobile phase temp inside column
Oven Temp
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Quick Warmup - Outstanding Stability
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8:00
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9:00
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9:30
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Time
Te
mp
era
ture
(d
eg
ree
s C
)
Ambient Room
Column heater
Column
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Reproducibility of 50 Injections
Reproducibility <0.8% over 50 injections and 12 hours
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Varian 920-LC Software Usability
• User friendly GalaxieTM software
• Simplified, modern, consistent Instrument interface
• Modular 11 pages
• 920-LC 4 pages!!
• One page real-time status and chromatogram
• Advanced integration and calibration
• New IMS capabilities
• Automated instrument validation
• Reduces downtime and operating costs
• Fusion AE
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Varian 920-LC Integrated HPLC
Special features of the Varian 920-LC• Leak detector
• Increases confidence with unattended automated runs
• Single waste point• Ensures capture of all wastes
• Easy to connect to waste vessel
• Built-in Solvent bottle storage• Improved safety and convenience
• Lower hight than modular systems
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Varian 920-LC Integrated HPLC
Special features of the Varian 920-LC• Clear front doors
• Stylish
• Provides access to autosampler without opening
• Safety increased as lab staff shielded from solvent spray and leaks
• Useful, convenient storage compartments• Column rack in door
• Tool drawer
• Operation log drawer
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Varian 920-LC AIQ (Validation)
AIQ (Analytical Instrument Qualification)• Fully PC controlled IQ and OQ (not PQ)
• Puts the Varian 920-LC at the forefront of regulated capabilities
• Consistent, reproducible methodology• Includes pumps, autosampler, detectors, column
heater
• Chargeable option
• Fully documented
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900 LC -Series AIQ
• Full system test designed and implemented as part of the 900-LC Series Analytical Instrument Qualification package.
• Verifies the entire LC system by systematically running the following
• Flow Rate Accuracy Test
• Wavelength Detector Accuracy Test
• Auto-sampler Injector Accuracy Test (also measures Detector Linearity)
• Auto-sampler Injector Precision Test
• Auto-sampler Injector Carryover Test
• Proportioning Accuracy Test
• Detector Noise Test
• Detector Drift Test
• Auto-sampler Vial Identification Accuracy Test
• Proportioning Linearity
• Compositional Ripple
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Varian 920-LC Configurations
pump inject detector Oven Detector 2
option
Options
Isocratic
00-100890-00Dual piston pump 10 ml head
Auto Dual UV/Vis
Opt FL, RI ELSD
Gradient
00-100891-00Quat, Grad, Degas Auto Dual
UV/VisOpt FL, RI ELSD opt
High Performance Gradient
00-100892-00
Quat, Grad, Degas Auto cooled Dual UV/Vis
Opt FL, RI ELSD opt
Development
00-100893-00Quat, Grad, Degas Auto PDA Std FL, RI ELSD opt
Research
00-100894-00Quat, Grad, Degas Auto cooled PDA Std FL, RI ELSD opt
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Varian 920-LC Consumables
• Excellent range of HPLC Columns providing industry leading resolution and robustness
• Pursuit
• Pursuit XRS
• Polymeric Phases - PL
• From 2mm to 2” ID Prepacked
• Other Speciality Phases
• NEW 2.8 Ultra Column AND 2.4um Columns for Fast or High Res LC
• 2.8um Ultra is optimised for Speed
• 2.4um gives further enhancements in Resolution
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2.8um Vs 2.4 um – High Res or Extra Speed
2.8um 2mm x 5cm for speed
2.4um 3mm x 5cm for RESOLUTION
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Questions??