maximize your hplc efficiency...maximize your hplc efficiency infinitylab lc series gregory hunlen...

Maximize your HPLC

Efficiency

InfinityLab LC Series

Gregory Hunlen

Application Scientist

November 2016

1

The Agilent InfinityLab LC Series!

1260 Infinity II LC1290 Infinity II LC 1220 Infinity II LC

Sept. 2014 May 2016 Oct 2016

Agilent is completely renewing it’s analytical LC portfolio!

November 2016

2

InfinityLab LC Series – a Remarkable Pedigree

Evolution not Revolution – but with revolutionary technology

To be consistent with existing workflows is of utmost importance for most of our customers.

Agilent strictly focus to introduce innovative and revolutionary technologies always with backwards

compatibility in mind. Backwards compatible with existing modules and with methods!

November 2016

3

New Benchmarks in Efficiency

The Agilent 1290 Infinity II LC, the next generation in Ultra High

Performance Liquid Chromatography, raises the efficiency of your

business in three dimensions:

Maximize Analytical Efficiency

Unmatched separation and detection performance deliver analysis data of

the highest quality—for ultimate confidence in your results.

Maximize Instrument Efficiency

Highest sample capacity and fastest injection cycles combine with new

levels of usability—for highest throughput for any application.

Maximize Laboratory Efficiency

Seamless integration in current infrastructure and smooth method transfer

from legacy equipment—for non-disruptive transition to highest productivity

and lowest cost of ownership.

Agilent 1290 Infinity II LC

November 2016

4

New 1290 Infinity II VWD

New 1290 Infinity II Multisampler

New 1290 Infinity II High Speed Pump

New 1290 Infinity II Flexible Pump New Accessories New 1290 Infinity II ELSD

New 1290 Infinity II Multicolumn Thermostat New 1290 Infinity II DAD

New 1290 Infinity II DAD-FS

The New Agilent 1290 Infinity II LC

November 2016

5

Flexibility for all applications

1290 Infinity II Flexible/High Speed Pump

• Wide power range

• Gradient options for higher speed or flexibility

1290 Infinity II Multisampler

• Dual-needle setup for scalable injection volumes

1290 Infinity II Multicolumn Thermostat

• Column temperature range from 4 to 110 °C

Intelligent System Emulation

Technology to run legacy

methods – even from other

vendor’s LCs

November 2016

6

Wide power range up to 1300 bar (2ml/min)/800 bar

(5ml/min) for optimum use of all analytical columns

independent of inner-diameter or particle technology


November 2016

7

1290 Infinity II Pumps Comparison

8

1290 Infinity II High Speed Pump

• 2 Channels

• Power Range: 2ml/min (1300bar), 5ml/min

(800bar)

• 45µl Standard Delay Volume

• For ultrafast gradients

• Best front-end for MS Users (2.1mm ID

col., low flow)

• Top-choice for binary pump lovers

• Includes ISET

1290 Infinity II Flexible Pump

• 4 Channels

• Power Range: 2ml/min (1300bar), 5ml

(800bar)

• <350µl Standard Delay Volume

• Flexibility in gradient formation

• Buffer blending

• Method development

• No ultrafast methods, where delay volume

is critical!

• Unmatched accuracy and application

range

November 2016


Highest retention time precision for reliable

peak identificationThe 1290 Infinity II Flexible Pump is the only low-

pressure mixing pump that combines the ultimate

performance of a high-pressure mixing UHPLC pump with

the flexibility of a quaternary pump. This pump delivers

superior gradient performance over the entire composition

range from 1 to 99 %.

November 2016

9

Highest retention time precision for reliable

peak identification

Isocratic analysis

The 1290 Infinity II Flexible Pump is the only low-

pressure mixing pump that combines the ultimate

performance of a high-pressure mixing UHPLC pump with

the flexibility of a quaternary pump. This pump delivers

superior gradient performance over the entire composition

range from 1 to 99 %.

November 2016

10


The dual-needle setup of the 1290 Infinity II Multisampler

enhances flexibility by providing two injectors in a single

instrument. You can optimize one path for large volume

injections and the other for low delay volumes.

20µl

500µl

November 2016

11

Lowest carryover for highest data quality

Wash inner needle

Flow through principle washes inner needle

thoroughly and constant with eluent.

Wash outer needle

Choice of 3 external solvents to program

washing steps for the outer needle.

Needle-seat back flush

Choice of 3 external solvents to back

flush the seat.

Using the new Multiwash feature you can flush the inside

and outside surfaces of the injection needle with up to three

different solvents, as well as backflush the needle seat.

November 2016

12

Lowest carryover for highest data quality

The 1290 Infinity II Multisampler with multiwash reduces

carryover to less than 9 ppm!

November 2016

13

How is Sample Management facilitated?

Agilent’s new Sample Entry Component uses intuitive graphical user interaction

similar to graphic/paint programs which allow sample list and sequence setups

based on sample container layouts. The component is autosampler and sample

container independent and can adapt to any sampler or container and geometry

via usage of the build-in and published proprietary description interfaces. For any sampler or container type

14

Simple and effectively to use Graphical tool for sample entry

July 1, 2016

The NEW Agilent InfinityLab LC Series - India

Laboratory Efficiency – Invest into new LCs and software to finally save money!

The new Agilent 1260 Infinity II LC!

November 2016

15


New Electronics (future proof, RoHS compatible)

Leveraging 1290 Infinity II LC technologies into the core platform, for your benefit!

New Housing (better ergonomics, higher safety

standard)

New Autosamplers (significantly better performance)

New Column compartments (highest usability or

price optimized)

New Detectors (improved specifications)

New Pumps (new functions and features)

New Accessories (better usability, better performance)

With full backwards method compatibility!

November 2016

16

November 2016

1260 Infinity II LC: 18 New Modules

17

* Available for ordering 01.Aug.2016

** Available for ordering Q1 2017

1260 Infinity II VWD

1260 Infinity II

Multisampler

1260 Infinity II

Binary Pump*

1260 Infinity II

Quarternary Pump

1260 Infinity II

Multicolumn Thermostat

1260 Infinity II DAD-HS

1260 Infinity II DAD-WR1260 Infinity II

Vialsampler

1260 Infinity II Bio-

inert Multisampler

1260 Infinity II Bio-inert

Quarternary Pump

1260 Infinity II FLD

1260 Infinity II RID

1260 Infinity II

Isocratic Pump

1260 Infinity II MWD

1260 Infinity II

SFC Multisampler**

1260 Infinity II

SFC Binary Pump**

1260 Infinity II FLD Spectra

1260 Infinity II

ELSD*

Infinity II Vialsampler

• drawer-like vial-trays with convenient access

• 2 individual trays for loading during analysis

• 2 x 66 vials = 132 vials

(cartesian arrangement A1-F11)

• Optional „classic vial tray 100“

(numbering 1-50 and 51-100)

Vial trays (default = 2x 66 vials 2 mL)

Needle-wash Station

• Outer Needle Wash for carryover reduction

• Carryover <40 ppm

18

November 2016

Infinity II Vialsampler: integration

Sample Cooler option

• 5°C below ambient down to 4°C

• Accuracy +/- 2°C

• Slide in cooler from backside

• Customer installable

• No heating capability

Integrated Column Compartment (ICC) option

• Optional upgrade, user exchangable

• Column heating (+5°C above ambient to 80°C)

• up to 2 Columns (max 7 mm ID, max 30 cm lenght)

• 2 Versions: ICC with 3 µl or 6 µL heater volume

• Hinged door for convenient column installation

19

November 2016

Infinity II Vialsampler: easy access

Walk-Up option

• External tray Option

• up to 5 vials

• Automated loading and diposal

• Supported by OpenLAB and MassHunter

20

November 2016

Laboratory Efficiency – Invest into new LCs and software to finally save money!


November 2016

21

The Agilent 1220 Infinity II LC600 bar for UHPLC Capabilities!

Isocratic or Gradient Pump:• 600 bar

• flow range: 0.2 - 10 mL/min

• low pressure mixing

• integrated degasser

• flow precision 0.07 % RSD

• AIV available NEW

Manual Injector or Autosampler:• injection range: 0.1-100 µL, extension up to

1500 µL

• 100 x 2 mL vials or 30 x 6 mL vials

• injection cycle time 50 s

• carryover: < 0.05%

• SIM sampler cooler 5-40°C coming soon

Column Oven:• up to 80°C

• one column, max. 250 mm

VWD or DAD:• 80 Hz data rate

• wavelength range: 190 - 600 nm (VWD)

up to 950 nm (DAD)

• 2.5 µAU (7 µAU for DAD) noise at 230 nm

November 2016

22

1220 Infinity II LC Mobile SolutionThe only transportation-optimized UHPLC system in the world !!!

The 1220 Infinity II LC in combination with a Mobile

Upgrade Kit for shock & vibration resistance.

It can be placed in a van for on-site LC analysis.

November 2016

23

100% Method TransferabilitySwitching from 1100 Series to 1220/1260 Infinity LC

100% HPLC compatibility: On one system you can run your established and

validated HPLC methods – unchanged – as well as push UHPLC to new levels of

performance.

November 2016

24

The Story of the Infinity II Series

is the Story of Efficiencies

November 2016

25


New 1290 Infinity II Multicolumn Thermostat with space

for up to eight short columns, four 30 cm columns or

any combinations plus column selection valves allows

simplest method-switching.

November 2016

26


Wide column temperature range of the 1290 Infinity II

Multicolumn Thermostat from 4 to 110 °C to tune

selectivity or speed.

November 2016

27

A new level of usability


• Agilent A-Line Quick Connect UHPLC column

fittings for dead-volume-free fluidic connections

• New multicolumn thermostat with simplified access

and space for up to 8 columns

• Easy-to-install Quick Change heat exchangers with

volumes of 1.0, 1.6 or 3.0 μL

• Quick-Change valves for straightforward

reconfiguration of your switching schemes

Graphical sample entry for easy

and fast sequence setup

November 2016

28


New Agilent A-Line Quick Connect UHPLC column fittings

for truly dead-volume-free fluidic connections

• Tool-free connection up to 1300 bar

• Removable and re-usable for all column types

• No overtighting of threads and damaging columns

November 2016

29

Designed-In Supplies for 1290 Infinity II

The Agilent A-Line Quick Connect Fitting

November 2016

30

• Finger tight up to 1300 Bar

• Spring loaded design

• Design with lever does not damage the capillary

• High efficiency for everybody

• Leak free and reliable connection

• Multiple connections

• Ease of use for non experts

• ZDV connection for all column types

• Removable and reusable

Features

Advantages

Benefits

LE

SS

ST

RE

SS

MO

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SU

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SA

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The Agilent A-Line Quick Turn Fitting

November 2016

31

• Finger tight up to 600 Bar, with a wrench up to 1300 Bar

• Spring loaded design

• Design does not damage the capillary

• Leak free and reliable connection

• Multiple connections

• ZDV connection for all column types

• Removable and reusable

Features

Advantages

Benefits

Swagelok

compatible


New 1290 Infinity II Multicolumn Thermostat with

simplified access and space for up to eight columns

November 2016

32


New 1290 Infinity II Multicolumn Thermostat with

simplified access, easy and flexible to install Quick-

Change heat-exchangers and Quick-Change Valves. Plus,

unmatched ease of column installation – done in seconds!

November 2016

33


New easy-to-install Quick Change heat exchangers with

volumes of 1.0, 1.6 or 3.0 μL.

November 2016

34


Increased Quick-Change valve portfolio for straightforward

reconfiguration of your flow path.

(1290 Infinity TCC shown)

November 2016

35


The Agilent A-Line Stay Safe Cap

November 2016

36

• Tight system

• Free rotating cap

• Ergonomic design

• Time strip on venting valve

• Health lab environment

• Easy solvent refill

• Fast tube connection

• Efficient use of valve

• No solvent vapors in the lab

• No twisted solvent tubes

• Hand tight connection

• Information on valve usage

Features

Advantages

Benefits

A-Line Stay Safe Cap Starter Kit 5043-1222

Includes

• 3 caps with 1port

• 1 cap with 2 ports

• 4 venting valves

• 4 fittings

Available as an

instrument option

November 2016

37

• Robust design

• Adjustable shelves

• Handle grip

• Power strip

• Container for waste bottle

• Stable system

• Easy LC stack customization

• Customer self-assembling

• No risk when moving

• High flexibility

• Easy handling

Features

Advantages

Benefits

Designed-In Supplies for 1290 Infinity IIThe Agilent A-Line Mobile Flex Bench

POIS

Opportunity

5043-1252 A-Line Mobile Flex Bench

79cm

165cm

79cm

CPL: November 01

More automation capabilities

1290 Infinity II Multisampler

• Injector programming for simple sample preparation

steps such as dilution, mixing or derivatization


• Quick-Change valves for column and solvent

selection, column regeneration, or sample clean-up

and enrichment

Method Scouting Wizard for easy

method & sequence generation for

column/solvent screening

BlendAssist software for

convenient, accurate and precise

additive or buffer blending

November 2016

38

Detection capabilities

1290 Infinity II Diode Array Detector

• 60 mm Max-Light Flow Cell with Optofluidic

Waveguides

• 30 x wider linear range for samples with

widely different concentration levels with

HDR-DAD

1290 Infinity II ELSD

• 9 x wider linear range for samples with widely

different concentration levels

• Detection of compounds without chromophor

1290 Infinity II VWD

• 240 Hz data rate

• Dual wavelength capability

Unique detection capabilities reveal more

November 2016

39

Leading 2D-LC Solutions

Most versatile ISET technology and methoddevelopment solutions

Most robust Long Life pumphead technologyand Mixing

Maximum Sampling Capacity with Minimum Benchspace Requirements

High Dynamic Range DAD with High Sensitivity Maxlight Flow Cell Technology

The Agilent 1290 Infinity II LC - Leading in U(H)PLC

Technology

1290 Infinity II LC

November 2016

40

Innovation: 1290 Infinity II 2D-LC SolutionSquare your Separation

1290 Infinity II 2D-LC Solution

• Analytical Efficiency

Unmatched levels of separation

power to solve your most

complex separation problems

• Instrument Efficiency

Most easy-to-use software and

easiest start into 2D-LC with

Easy Starter Kit

• Laboratory Efficiency

Fully scalable system with

automatic switch between 1D-

LC, (Multiple) Heart-Cutting,

and Comprehensive 2D-LC

WHY 2D-LC?

From 1D-HPLC to 2D-LC

November 17, 2016

Confidentiality Label

42

General thoughts in separation science

43

„Resolving power is what it is all

about in analytical separation

science.“

November 17, 2016

Taxanes from taxus extractWhere 2D-LC can help

• Taxol® (Paclitaxel) excellent antitumor activity against

breast and lung cancer.

• Content in Taxus brevifolia very low, synthesis very difficult due

to complex structure.

• Monitoring of content in crop cultures highly desired, but

difficult due to complex herbal matrix

Application Note 5991-3576EN.pdf1D-Chromatograms of standard mix and plant extracts

44

November 17, 2016

http://en.wikipedia.org/wiki/File:Taxol.svg

http://en.wikipedia.org/wiki/File:Taxol.svg

http://www.chem.agilent.com/Library/applications/5991-3576EN.pdf

Bringing in a 2nd Dimension - how?

45

Pump Autosampler Column Detector

„Just like 1D-LC with a chemically selective detector“

DAD = separates in the wavelength-domain

MSD = separates in the mass-domain

„CSD“ = separates in the chemical domain (again...)

Detector

November 17, 2016

Bringing in a 2nd Dimension - how?

46

Pump Autosampler Column Detector

Pump

Detector

Co

lum

n

Valve

(„Sampler“)

1D-LC System

2D-LC System

+

1D-LC System

„Just like 1D-LC with a chemically selective detector“

November 17, 2016

1D-Setup

Switchable 1D/2D SetupChange between UHPLC and 2D-LC without manual Interaction

2D-Setup

47

November 17, 2016

Technical Note 5991-4843EN

https://www.chem.agilent.com/Library/technicaloverviews/Public/5991-4843EN.pdf

What is ISET?- Two components

A. Best in class performance of the

1290 Infinity LCBroad power range, ultra-low delay volume,

superior sensitivity, unmatched flow and

composition accuracy

B. The revolutionary ISET emulation

algorithmFull characterization of target LC systems

Creation of emulation function

FA

Peak 3

Overlay of 10 runs

RSD = 0.005 %

November 2016

What is ISET?

With ISET – part of the 1290 Infinity II pumps – you can

execute any legacy HPLC or latest UHPLC method while

delivering the same chromatographic results.

ISET eliminates deviations in resolution and retention times

when your laboratory deploys methods developed originally

on other HPLC or UHPLC systems.

November 2016

49

Intelligent System emulation technology- How it works (1290 to 1200)

Programed gradient

1290 gradient

1200 gradient

0 0.5 1 1.5 2 2.5 3 3.5

0

50

100

150

200

250

300

350

400

min

mAU

Injection

Same gradient conditions by moving

the gradient and by emulating the

gradient mixing behavior.

November 2016

Select pump

Select sampler

Intelligent System Emulation Technology ISETImplementation

November 2016

51

Intelligent System Emulation Technology ISET- How to emulate Waters Alliance?

Select vendor

Select system

Select sample

loop

November 2016

52

ISET not only takes care for the various delay volumes in

different LC systems but also for different mixing behavior

– this is only available from Agilent and make instrument-

to-instrument method transfer easier than ever before.

Non-disruptive transition by ISET

November 2016

54

Agilent innovative ISET technology allows you to use legacy

methods of virtually any LC and achieve the same results on

latest 1290 Infinity II LCs. You can retire your old systems

without retiring your precious methods.

All 1290 Infinity II pumps have ISET included.

Non-disruptive transition by ISET

November 2016

55


The 60 mm path length of the Agilent Max-Light flow cell

enables the 1290 Infinity II DAD to achieve typical noise

levels of less than ± 0.6 μAU/cm for highest detection

sensitivity and lowest detection limits.

1290 Infinity II DAD with

High Sensitivity cell

Conventional detector

November 2016

56

Effects of path length increase

Peak Dispersion

60 mm pathlength

large geom. cell volume (78 µL)

lower light transmission

- Loss of resolution

- Loss of signal height

10 mm pathlength

small geom. cell volume (13 µL)

high light transmission

Conventional flow cells:

60 mm pathlength

4 µL σV dispersion volume

Max-Light High Sensitivity cell:

Optofluidic waveguides

(total internal reflection)


For your samples with widely different concentration levels,

the 1290 Infinity II High Dynamic Range DAD solution

offers a 30x wider linear range and up to 30x higher sensitivity,

allowing you to detect and quantify all components within a

single run.

November 2016

58


For your samples with widely different concentration levels,

the 1290 Infinity II High Dynamic Range DAD solution

offers a 30x wider linear range and up to 30x higher sensitivity,

allowing you to detect and quantify all components within a

single run.

November 2016

59

Introducing Agilent InfinityLab

Combined with Agilent OpenLAB Software and Agilent CrossLab Services & Support, Agilent

provides you with complete Liquid Chromatography solutions which make a real difference.

OpenLAB

Software solutions to capture,

analyze, and share analytical data.- Chromatographic Data Systems

- Enterprise Content Management

- Electronic Notebook

- MassHunter MS Solutions

InfinityLab

LC components designed

to perfectly fit together.- Instruments

- Columns

- Supplies

CrossLab

Serving more than 260000 labs

around the globe.- Instrument repair and maintenance

- Compliance Solutions

- Training and Consulting

- Laboratory Enterprise Solutions

November 2016

60

Society Needs More Results for a Better Quality of Life

November 2016

61

Data Analysis and Interpretation Reporting SamplePreparation

Chromatography

Today’s workflow

Highlights

Agilent InfinityLab

LC Series enables

more with less!

Future workflow

Data Analysis and Interpretation Reporting SamplePreparation

Chromatography

The Story of the

InfinityLab LC Series

November 2016

62

Question?

maximize your hplc efficiency...maximize your hplc efficiency infinitylab lc series gregory hunlen...

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