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Carlos González Alonso Global Technical & Marketing Equipment Manager [email protected] Vaccination at the Hatchery INJECTION Key factors for process-control

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Page 1: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

Carlos González Alonso

Global Technical & Marketing Equipment Manager

[email protected]

Vaccination at the Hatchery

INJECTIONKey factors for process-control

Page 2: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

Hatchery Injection Practices - 2012

51 bill.Broilers

Page 3: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

Injection EquipmentMain Figures

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Page 4: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

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Injection EquipmentMain Figures

Page 5: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

What are the

Key Factors for Control

in SQ Vaccination?

Page 6: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

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CONCEPT SQ

Optimal Vaccination Efficacy +98%

Maximum Recommended Speed

2500-3000 DOCs/hour

Maximum Recommended Time of Operation

6 hours/operator

Maximum Output/Day 18.000 DOCs/operator/day

Recommended Maintenance 1.5 hour/SQ vaccinator/week

Sub-Cutaneous Injection

Page 7: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

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MAIN TOPICS TO CONSIDER Key Factors

Equipment Setting • Needle: length, orientation and size• Performance: Dosage, accuracy, etc.• Equipment maintenance

Operator Skills & Training • DOC handling technique• Speed impact and working hours• HR availability and turn-over

Supervision & Monitoring • Vaccination Efficacy per operator• Dosage control• Logistics & Organization

Vaccine Handling & Status • Vaccine preparation • Timing and vaccine stability• Impact of equipment on vaccine status

Biosecurity & Contamination • Monitoring of vaccine contamination• Crossed contamination between DOC• Environmental contamination

Sub-Cutaneous Injection

Page 8: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

Sub-Cutaneous Injection

Self Injection

Low Accuracy

Unconsistent location

Page 9: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

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Sub-Cutaneous Injection

Page 10: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

[email protected]

MAIN TOPICS TO CONSIDER Key Factors

Equipment Setting • Needle: length, orientation and size• Performance: Dosage, accuracy, etc.• Equipment maintenance

Sub-Cutaneous Injection

Page 11: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

[email protected]

Sub-Cutaneous Injection

Page 12: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

[email protected]

Sub-Cutaneous Injection

Page 13: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

[email protected]

MAIN TOPICS TO CONSIDER Key Factors

Operator skills & training • DOC handling technique• Speed impact and working hours impact• HR availability and turn-over

Sub-Cutaneous Injection

Page 14: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

[email protected]

Sub-Cutaneous Injection

Page 15: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

[email protected]

Sub-Cutaneous Injection

Page 16: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

[email protected]

Sub-Cutaneous Injection

Page 17: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

[email protected]

Sub-Cutaneous Injection

Page 18: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

[email protected]

Sub-Cutaneous Injection

Page 19: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

[email protected]

Sub-Cutaneous Injection

Page 20: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

[email protected]

Sub-Cutaneous Injection

VACCINATIONFAILURE

REFERENCEINCIDENCE

2012 DATA (avg.)

IMPACT

No Vaccine <0.5% 1.29% No protection in the fieldVaccine waste

Wet Fluff <0.5% 2.17% Low or no protectionVaccine waste

Mal-position <0.5% 3.81% Low absorption of the vaccineRisk of damages of vascular systemRisk of damages on digestive tract

Blood <0.25% 0.44% Infection of damaged tissuesHigher first week mortality

Bone-damage <0.25% 0.16% Injection at crane or back-spineCull chicks; Mortality

Vaccination Q > 98% 92.13%

Page 21: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

Sub-Cutaneous Injection

Page 22: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

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MAIN TOPICS TO CONSIDER Key Factors

Supervision & Monitoring • Vaccination Efficacy per operator• Dosage control• Logistics & Organization

Sub-Cutaneous Injection

0.00%

1.00%

2.00%

3.00%

4.00%

5.00%

6.00%

7.00%

8.00%

9.00%

10.00%

M ojado M od. 5.73% 4.83% 9.00% 7.90% 5.50% 3.54% 3.29% 2.56% 2.89% 3.18% 3.77% 2.14% 0.60% 2.06% 1.67%

M ojado Sev. 6.18% 3.83% 2.86% 2.80% 1.50% 2.12% 1.59% 0.78% 0.93% 0.30% 1.09% 0.36% 2.53% 0.50% 0.86%

Heridos 1.09% 1.25% 1.14% 0.80% 1.56% 0.92% 1.06% 0.56% 0.43% 0.57% 0.59% 0.50% 0.60% 1.33% 0.33%

M ala Posición 0.45% 0.58% 1.43% 0.60% 0.81% 0.19% 0.41% 0.00% 0.55% 0.27% 0.55% 0.14% 0.00% 0.39% 0.29%

No vacunados 0.00% 0.00% 0.86% 1.10% 0.31% 0.31% 0.47% 0.00% 0.05% 0.07% 0.09% 0.00% 0.33% 0.06% 0.14%

M uertos 0.00% 0.00% 0.00% 0.00% 0.00% 0.04% 0.00% 0.00% 0.05% 0.05% 0.05% 0.00% 0.00% 0.00% 0.00%

16-Oct 23-Oct 30-Oct 06-Nov 13-Nov 20-Nov 21-Nov 01-Dic 11-Dic 12-Dic 18-Dic 30-Dic 15-Ene 29-Ene 10-Feb

Data CollectionData Analysis

Page 23: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

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Sub-Cutaneous Injection

0.00%

1.00%

2.00%

3.00%

4.00%

5.00%

6.00%

7.00%

8.00%

9.00%

10.00%

M ojado M od. 5.73% 4.83% 9.00% 7.90% 5.50% 3.54% 3.29% 2.56% 2.89% 3.18% 3.77% 2.14% 0.60% 2.06% 1.67%

M ojado Sev. 6.18% 3.83% 2.86% 2.80% 1.50% 2.12% 1.59% 0.78% 0.93% 0.30% 1.09% 0.36% 2.53% 0.50% 0.86%

Heridos 1.09% 1.25% 1.14% 0.80% 1.56% 0.92% 1.06% 0.56% 0.43% 0.57% 0.59% 0.50% 0.60% 1.33% 0.33%

M ala Posición 0.45% 0.58% 1.43% 0.60% 0.81% 0.19% 0.41% 0.00% 0.55% 0.27% 0.55% 0.14% 0.00% 0.39% 0.29%

No vacunados 0.00% 0.00% 0.86% 1.10% 0.31% 0.31% 0.47% 0.00% 0.05% 0.07% 0.09% 0.00% 0.33% 0.06% 0.14%

M uertos 0.00% 0.00% 0.00% 0.00% 0.00% 0.04% 0.00% 0.00% 0.05% 0.05% 0.05% 0.00% 0.00% 0.00% 0.00%

16-Oct 23-Oct 30-Oct 06-Nov 13-Nov 20-Nov 21-Nov 01-Dic 11-Dic 12-Dic 18-Dic 30-Dic 15-Ene 29-Ene 10-Feb

Page 24: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

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Sub-Cutaneous Injection

97,25%

95,33%

98,67%

93,00%

84,00%

99,00%

82,00%

96,40%

98,00%

95,90%

96,33%

98,50%

98,33%

98,67%

60%

65%

70%

75%

80%

85%

90%

95%

100%

16

-Oc

t

23

-Oc

t

30

-Oc

t

06

-No

v

13

-No

v

20

-No

v

21

-No

v

01

-dé

c

11

-Dic

12

-Dic

18

-Dic

30

-Dic

15

-En

e

29

-En

e

10

-Fe

b

Vaccinator A

Dates of Audits

Vaccin

ation Q

ualit

y

Page 25: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

[email protected]

MAIN TOPICS TO CONSIDER Key Factors

Supervision & Monitoring • Vaccination Efficacy per operator• Dosage control• Logistics & Organization

Sub-Cutaneous Injection

Page 26: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

[email protected]

Sub-Cutaneous Injection

Page 27: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

[email protected]

Sub-Cutaneous Injection

Page 28: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

[email protected]

Sub-Cutaneous Injection

Page 29: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

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MAIN TOPICS TO CONSIDER Key Factors

Vaccine handling & status • Vaccine preparation • Timing and vaccine stability• Impact of equipment on vaccine status

Sub-Cutaneous Injection

Vaccine titter

loss due to

vaccine tubes

set-up

Page 30: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

Sub-Cutaneous Injection

0

1000

2000

3000

4000

5000

6000

7000

8000

PF

U/D

OS

E

7000

6000

5000

4000

3000

2000

1000

0

BAG #1

BAG #2

BAG #3

BAG MACHINE - NEAR MACHINE - FAR

Vaccine titter loss due to vaccine tubes set-up

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Page 31: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

[email protected]

MAIN TOPICS TO CONSIDER Key Factors

Biosecurity & Contamination • Monitoring of vaccine contamination• Cross contamination between DOC• Environmental contamination

Sub-Cutaneous Injection

Page 32: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

[email protected]

MAIN TOPICS TO CONSIDER Key Factors

Biosecurity & Contamination • Monitoring of vaccine contamination• Crossed contamination between DOC• Environmental contamination

Sub-Cutaneous Injection

Page 33: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

Sub-Cutaneous Injection

MAIN TOPICS TO CONSIDER Key Factors

Biosecurity & Contamination • Monitoring of vaccine contamination• Crossed contamination between DOC• Environmental contamination

Page 34: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

SQ is a simple method, but it

demads skilled operators and

constant monitoring

Page 35: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

What are the

Key Factors for Control

in In-Ovo Vaccination?

Page 36: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

Monitoring In-Ovo Vaccination

Page 37: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

Monitoring In-Ovo Vaccination

Page 38: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

Monitoring In-Ovo Vaccination

Page 39: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

Monitoring In-Ovo Vaccination

Site of Location Test

Page 40: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

Monitoring In-Ovo Vaccination

Page 41: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

Ideal

Medium

Poor

Early

Embryo development days2017 18 19

Monitoring In-Ovo Vaccination

Page 42: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

Monitoring In-Ovo Vaccination

Page 43: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

Monitoring In-Ovo Vaccination

Page 44: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

Monitoring In-Ovo Vaccination

Page 45: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

Microbiological Monitoring

• Intensive monitoring of the hatchery and the vaccination process.

• Aspergillus sp. incidence and all major production-related bacteria

(E.Coli, Pseudomonas sp., Salmonella sp., Staphilococus sp., etc…)

• Helps to improve the bio-sanitare status of the hatchery and

secures the in-ovo vaccination process.

Vaccination Micro Control

Regular micro monitoring evaluation of the

vaccination process

Hatchery Micro Control

Yearly monitoring of the hatchery plant

and all production processes

Monitoring In-Ovo Vaccination

Page 46: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

Monitoring In-Ovo Vaccination

Page 47: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

Monitoring In-Ovo Vaccination

Page 48: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

Monitoring In-Ovo Vaccination

Page 49: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

Monitoring In-Ovo Vaccination

Page 50: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

Concept

Young Flocks

(>30w)

Medium Flocks

(30-45w)

Old Flocks

(<45w)

Egg Breakage 0.4% 0.5% 0.5%

Upside-down Eggs 0.3% 0.2% 0.2%

Side Injections 0.25% 0.2% 0.2%

Abnormal Perforation <0.1% <0.1% <0.1%

Blocked Needles <8% 8% 8-10%

Contaminated Eggs 0.25% 0.3% 0.4%

Aspergillus sp. 0.5% 0.5% 0.8%

Monitoring In-Ovo Vaccination

It is KEY to create your own

reference data sheet

Page 51: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

Topic Recomended Frequency

Vaccine Prep. Daily

In-Ovo Checklist Daily

Dosage Control Daily

Microbiological Monitoring 15 days

Operator Training Continious

Embryo-diagnosis Daily2-3 flocks

Disinfection Mix Control Daily

Egg Cleaning & Sanitare Status Weekly

Hatchability Assessment Weekly 5-10 flocks

Monitoring In-Ovo Vaccination

Page 52: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

Summary

o Hatchery Injection represents 65-70% of

the total Broiler production world-wide with

a strong positive trend.

o Skilled operatos in equipment care and

vaccination technique are requiered for

success.

o Constant monitoring and data analysis

are key factors for improvement.

o The implementation of regular vaccination

services and monitoring activities is

mandatory.

Vaccination at the Hatchery

INJECTIONKey factors for process-control

Page 53: Vaccination at the Hatchery INJECTION - Transmune€¦ · Microbiological Monitoring • Intensive monitoring of the hatchery and the vaccination process. • Aspergillus sp. incidence

Together, beyond animal health

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