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Page 1: UNITED STATES ARMY NATICK, MASSACHUSETTS 01760 … · and spices: total ash, acid insoluble ash, volatile oil (expressed as ml/100g), non-volatile ether extract, ASTA color value,

I AL REPI

52 FEL

Approved for public release;

distribution unlimited.

Project Reference: O&MA 728012.19

June 1976

UNITED STATES ARMY"

NATICK, MASSACHUSETTS 01760

[ineering Laboratory

PEL 94

Page 2: UNITED STATES ARMY NATICK, MASSACHUSETTS 01760 … · and spices: total ash, acid insoluble ash, volatile oil (expressed as ml/100g), non-volatile ether extract, ASTA color value,

Approved for public release; distribution unlimited.

Citation of trade names in this report does not constitute an official indorsement or approval of the use of such items.

Destroy this report when no longer needed. Do not return it to the originator.

Page 3: UNITED STATES ARMY NATICK, MASSACHUSETTS 01760 … · and spices: total ash, acid insoluble ash, volatile oil (expressed as ml/100g), non-volatile ether extract, ASTA color value,

SECURITY CLASSIFICATION OF THIS PAGE (When Data Entered)

REPORT DOCUMENTATION PAGE READ INSTRUCTIONS BEFORE COMPLETING FORM

1. REPORT NUMBER

TR 76-52 FEL 2. GOVT ACCESSION NO. 3. RECIPIENT'S CATALOG NUMBER

4. TITLE (and Subtl tie)

"Quality Evaluation of Herbs and Spices in The Military Food System"

S. TYPE OF REPORT ft PERIOD COVERED

Technical Report

6. PERFORMING ORG. REPORT NUMBER FEL-54

7. AUTHORfsJ

Y. Masuoka; Ivor T. Nii; Edmund M. Powers 8. CONTRACT OR GRANT NUMBER(s)

9. PERFORMING ORGANIZATION NAME AND ADDRESS

US Army Natick Research and Development Command Natick, MA 01760

10. PROGRAM ELEMENT, PROJECT, TASK AREA ft WORK UNIT NUMBERS

O&MA 728012.19

1 1. CONTROLLING OFFICE NAME AND ADDRESS

Same as Above

12. REPORT DATE

June 1976 13. NUMBER OF PAGES

14. MONITORING AGENCY NAME ft AODRESSff/ dl Heren t from Controlling Otlice) 15. SECURITY CLASS, (of (his reporfj

Unclassified 15a. DECLASS1FI CATION/DOWN GRADING

SCHEDULE

NA 16. DISTRIBUTION STATEMENT (ol this Report)

Approved for public release; distribution unlimited.

17. DISTRIBUTION STATEMENT (of the abstract entered in Block 20, it different from Report)

18. SUPPLEMENTARY NOTES

19. KEY WORDS (Continue on reverse side il necessary and identity by block number)

HERBS PACKAGING VOLATILITY SPICES SEALED SYSTEMS VOLATILE LOSS CONDIMENTS MOISTURE TRANSFER MILITARY FEEDING SYSTEMS CONTAINERS MOISTURE FEEDING

20. ABSTRACT (Continue on reverse eida If neceeaary and Identity by block number)

The services made an official inquiry into the quality of herbs and spices in the Military supply system at the 12-13 January 1972 Armed Forces Product Eval- uation Committee (AFPEC) meeting. As a result of this inquiry, it was recom- mended that the US Army Natick Research and Development Command (NARADCOM) survey samples of herbs and spices from sixteen Continental United States (CONUS) Military supply locations to determine if they conformed to specifica- tion requirements. In addition to testing for specification conformance, NARADCOM used a sensory profile panel to evaluate aromatic strength and tested

DD,™TU1473 EDITION OF T NOV 65 rS OBSOLETE

SECURITY CLASSIFICATION OF THIS PAGE (When Date Entered)

Page 4: UNITED STATES ARMY NATICK, MASSACHUSETTS 01760 … · and spices: total ash, acid insoluble ash, volatile oil (expressed as ml/100g), non-volatile ether extract, ASTA color value,

SECURITY CLASSIFICATION OF THIS PAGE(B7ian DM* Entered)

for microbiological flora. Herbs and spices from a leading producer of condiments were used as control samples. Results indicated that many of the herbs and spices being used by the Services were only marginal in quality when compared with their control commercial counterparts.

SECURITY CLASSIFICATION OF THIS PAGEfHTien Dal» Entered)

Page 5: UNITED STATES ARMY NATICK, MASSACHUSETTS 01760 … · and spices: total ash, acid insoluble ash, volatile oil (expressed as ml/100g), non-volatile ether extract, ASTA color value,

TABLE OF CONTENTS

Page List of Tables and Figures 3

Introduction 4

Experimental Procedures 5

Results and Discussion 8

Conclusion 11

References 12

1,

Page 6: UNITED STATES ARMY NATICK, MASSACHUSETTS 01760 … · and spices: total ash, acid insoluble ash, volatile oil (expressed as ml/100g), non-volatile ether extract, ASTA color value,

PREFACE

The US Army Natick Research and Development Command has received complaints about the quality of herbs and spices in the supply system. However, there is a lack of official documentation, unsatisfactory material reports (UMR's, etc). Part of the problem is undoubtly due to the limited use of hermetically sealed containers Co package these items. The commercial dredge-top retail container does not offer complete protection against moisture transfer, loss of volatiles, and the lik@D Compounding this problem, condiments with the exception of ground black pepper are locally procured items, or "X" coded. Local procurement permits government buying of products of a value up to $2500.00 as small local purchases without inspection to verify that specification requirements have been met«, Since the value of most spices and herbs is solely a function of the volatile and non-volatile flavors and analyses for these characteristics are seldom conducted* it is possible that the Government may be receiving something less than it should. The following study was initiated to explore the above problem and to propose changes to improve this situation. This effort was undertaken under Department of Defense Production Engineering in support of stock fund food and food service items.

The authors acknowledge with thanks the assistance of the following personnel of the US Army Natick Research and Development Command:

1. The Flavor Profile Panel. 2. The Food Acceptance Group, Food Science Laboratory. 3. The Food Chemistry Group, Food Sciences Laboratory. 4. The Food Microbiology Group, Food Sciences Laboratory.

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LIST OF TABLES

Table

1. Location of Continental United States (CQNUS) Bases submitting 13» Samples»

2. Chemical Analysis of Bay Leaves. 14» 3. Chemical Analysis of Garlic Powder, 15» 4. Chemical Analysis of Ground Cinnamon» 16. 5» Chemical Analysis of Oregano«, 17» 6. Chemical Analysis of Chili Powder» 18» 7. Chemical Analysis of Ground Mustard» 19» 8» Moisture Analysis and Scoville Pungency Ratings of Cayenne Pepper» 20» 9. Profile Panel Evaluation of Spices and Herbs. 21»

10» Quality of Bay Leaves as Related to Chemical Analysis, Profile 22« Evaluations, and Packaging Media,

11» Quality of Garlic Powder as Related to Chemical Analysis, Profile 23° Evaluations and Packaging Media,

12» Quality of Cinnamon Powder as Related to Chemical Analysis, Profile 24« Evaluations and Packaging Media,

13. Quality of Oregano Leaf & Powder as Related to Chemical Analysis, 26, Profile Evaluations and Packaging Media.

14. Quality of Chili Powder as Related to Chemical Analysis, ASTA Color, 28» Profile Evaluations and Packaging Media,

15. Quality of Ground Mustard as Related to Chemical Analysis, Profile 30» Evaluations and Packaging Media.

16. Quality of Red Pepper as Related to Chemical Analysis, Scoville 32. Ratings, Profile Evaluations and Packaging Media,

17. Microbiology of Processed Spices Used by the Armed Forces, 34» 18. Aerobic Plate Count (APC) of Processed Spices Used by the Armed 35.

Forces, 19» Yeast and Mold Count of Processed Spices Used by the Armed Forces» 36» 20» Coliform Count of Processed Spices Used by the Armed Forces, 37» 21» Clostridium Perfringens Count in Processed Spices Used by the 38.

Armed Forces, 22. Spices Containing Food Poisoning Bacteria and Califorms, 39» 23. Food Poisoning Bacteria and Coliforms Found in Different Brands 40»

of Spice.

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INTRODUCTION

The Armed Forces Product Evaluation Committee requested that the U.S, Army Natick Research and Development Command perform a quality audit of herbs and spices being supplied to the Military Services, Condiments» with the exception of ground black pepper, are locally purchased items, and aside from occasional Unsatisfactory Material Reports, very little feedback data are available to evaluate their quality in the supply system.

Each Military Service requested four installations to submit 6 samples each of ground cinnamon, ground Oregano* ground red pepper, ground mustard, chili powder, dehydrated garlic powder, and whole bay leaves with information on date of pack or date of receipt. These items represent the most widely used herbs and spices. They were sent to Natick R&DC in various commercial con- tainers such as glass bottles, paper bags, cans (both clinched and double seamed), cartons and chip-board boxes. The unit net weights ranged from 3/16 ounce to one pound«

Page 9: UNITED STATES ARMY NATICK, MASSACHUSETTS 01760 … · and spices: total ash, acid insoluble ash, volatile oil (expressed as ml/100g), non-volatile ether extract, ASTA color value,

EXPERIMENTAL PROCEDURES

A. Chemical Analyses. The following analyses were performed on the herbs and spices: total ash, acid insoluble ash, volatile oil (expressed as ml/100g), non-volatile ether extract, ASTA color value, and moisture. These methods are described in., the Official Analytical Methods of the American Spice Trade Association . The specific tests required for each herb or spice are referenced in the appropriate specifications for Chili Powder Seasoning , Onions and Garlic, Dehydrated , and Spices, Ground and Whole 4.

B. Scoville Pungency Evaluation. Fifteen samples of cayenne pepper and one sample of red pepper were tested using the Scoville (sensory) Heat Test of Pungency of Capsicum Spices and Oleoresins (ASTA Method 21.0) modified to denote a "presence" or "absence" of heat for each sample. Screened panels were presented a series of 4 dilutions in increasing strength; Included in each series was a sample at 45,000 Uftits (for cayenne pepper) and 31,000 Units (for red pepper). These pungency ratings are the minimum requirements set forth in Federal Specification EE-S-631G *, Three out of five judges recording positive responses at the minimum allowable pungency level constituted a "passing" sample. In this way, it was not necessary to determine the precise Scoville Value for each pepper but, to determine only If a pepper conformed to the specification requirements.

An alcoholic extract was made by adding 50 ml of 95% ethyl alcohol to 0.10 gram of ground pepper (Schedule A-Range C of ASTA-Method 21.0) and allowing a 20-24 hour extraction period. The extract was then filtered into a 50 ml volumetric flask and brought back to volume with 95% ethyl alcohol. For testing, the dilutions were prepared using a 5% sucrose solution (50 grams made up to a liter), made one hour prior to testing. The proper amount of alcoholic extract was placed in a 50 ml volumetric flask and diluted to the mark with sucrose solution.

For each test, a series of 4 dilutions as specified In Schedule B-ASTA-Method 21.0 was presented to each subject as follows:

Cayenne Pepper Extract Sample 1 Sample 2 Sample 3 S_ample__4

Scoville Units Blank 25,000 45,000* 60,000 ml of extract 0.00 1.00ml 0.55ml 0.42ml ml of sucrose (5% solution) 50ml 49.0ml 49.45ml 49.58ml *Note: For red pepper, 31,000 Scoville Units was substituted for 45,000 units.

Subjects were presented a 5 ml sample of all 4 dilutions at one time in 1 ounce plastic cups. An unsalted cracker and water were served to panel members to reduce flavor carry-over between tastings.

C. Profile Evaluation (spice strength). The standard profile technique was used to evaluate spice strength^.The flavor profile panel members (5-6 members per session) were instructed to report the aroma and flavor impressions and their strengths. These impressions and strengths were then converted to "good", "weak" and "absent". The samples were prepared by dilution to just-recognizable

5. -.1"!

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or threshold value as follows (Room temperature was used in all cases): Garlic-1; 1.000,000 dilution prepared in two steps by addingl-Bg^tö:-vlOOG'iiaUwat^r^ and letting diluent stand 2 hours. A one (1) ml aliquot of this concentrated solution was diluted further into 1000 ml water and.. let lefoand fdiri.2 hours^ej.sThis., represented •■ ah (aqueous. Solution'ofe^p.*p „m. garlic, Ciuuamon-0.05 g/100 g applesauce, One (1) g in 200 ml water. Let stand 2 hours. Dilute 10 ml of this solution with 90 g canned applesauce. Let stand 2 hours. Oregano-0.01 g/100 ml water. One (1) g in 200 ml water. Let stand 2 hours. Dilute two (2) ml of this solution with 98 ml water. Let stand 2 hours. Mustard-0.2 g/100 g chicken broth. Add 2 g in 100 ml water. Let stand 2 hours. Dilute 10 ml of this solution with 90 g of chicken broth. Let stand 2 hours. Red Pepper (cayenne pepper and red pepper).-0.02 g/100 g chicken broth» One (1) g in 100 ml water. Let stand 2 hours. Dilute 2 ml of this solution with 98.0 g chicken broth. Let stand 2 hours. Bay Leaves-0.02 g/100 ml water. 2 g in 100 ml water. Let stand 4 hours. Dilute one (1) ml of this solution in 100 ml water. Let stand,2 hours. Chili Powder-0.75 g/100 ml water. 5 g in 100 ml water. Let stand 2 hours. Dilute 1.5 ml of this solution in 100 ml water. Let stand 2 hours.

D. Microbiological Evaluations

Preparation of sample. Ten grams of each spice or herb were weighed into a ..i-sr-i sterile, tared blender jar and blended for 1 to 2 minutes in 90 ml of sterile phosphate buffered water (SBW)^.

Dilutions

All dilutions were made from the initial blend (1:10) by transferring 10 ml into 90 ml Butterfield Phosphate buffer-SBW.^-? The lowest dilution cultured for any count was 1:100 to avoid possible inhibition of growth due to bactericidal or bacteriostatic properties of some spices 8,9 # xn the case of Clostridium perfringens counts, this dilution was achieved by spreading 0.1 ml of 1:10 dilution on duplicate plates of Shahidi Ferguson Perfringens (SFP) agar.

Inhibition of Bacterial Growth by Spices

To determine whether the spices tested would inhibit bacterial growth, Eseheric- hia coli and Staphyloeoeeus aureus were inoculated in tubes of lauryl sulfate tryptose (LST) broth and trypticase soy broth (TSB) containing 10% sodium chloride (NaCl), respectively, to which was added 1 ml of 1:10 dilution of each spice. Excellent growth of both organisms was achieved in their respective media in less than 24 hours at 35°C, indicating that the spices were not in- hibitory at the concentration tested. Subsequent recovery of both organisms by standard methods (described below) was also achieved. To allow a margin of safety, the lowest "in-use" dilution of spices for all counting procedures was set at 1:100.

Media

All media were purchased from Difco Laboratories, Detroit, Michigan<,

6.

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Aerobic Plate Count

One ml dilutions ranging from 10 to 10"^ were pipetted into duplicate petri plates, poured with plate count agar, and incubated at 35°C for 48 hours.

Yeast and Mold Count

Dilutions prepared for aerobic plate counts were also used for making yeast and mold counts, One ml of each dilution was added to a petri plate and poured with potato dextrose agar (PDA), acidified to pH 3,5, Plates were incubated at 23°C for 5 days before counting,

Coliform and Fecal Coliform Count

Coliforms were estimated by a 3-tube probable number (MPN) determination in lauryl sulfate tryptose (LST) broth by standard methods**. Gas producing LST tubes were confirmed in brilliant green lactose bile (2%) broth (BGLB)„ Fecal coliforms were estimated by transferring growth from positive LST tubes to Escherichia coli (EC) broth at 45.5°C for 48 hours**. Counts, determined from MPN tables, are presented as 30/g when all tubes at the 1:100 dilution are negative.

Coagulase Positive Staphylococci

Coagulase positive staphylococci were estimated by a modification of the 3-tube MPN procedure by using, the same dilutions usedforthe coliform count. To al- low for recovery of injured cells, 1 ml of appropriate dilution was added to 5 ml of TSB and incubated at 35°C for 3 hours. An equal volume of TSB contain- ing 19.5% NaCl was then added to each tube to yield a final salt concentration of 10%7»10. After incubation at 35°C for 48 hours, 0.1 ml of the TSB-NaCl culture was spread on each of two plates of Vogel Johnson agar. Plates were incubated at 35°C and examined after 24 and 48 hours for the presence of smooth black colonies with yellow äones. Two or more typical representative colonies were transferred to brain heart infusion (BHI) tubes and tested for coagulase production according to standard procedures*** Counts, determined from MPN tables, are presented as 30/g when all tubes at the 1:100 dilution are negative.

Clostridium perfringens

Clostridium perfringens organisms were counted by spreading 0.1 ml of 1:10 and 1:100 dilutions onto duplicate plates of Shahidi-Ferguson perfringens (SFP) agar* . Plates were overlaid with 10 ml of SFP overlay agar (egg yolk omitted), placed into gas pack anaerobic jars (BBL), and incubated at 35°C for 24 hours, Black colonies surrounded by a zone of precipitate were counted and confirmed in lactose motility (LM) agar. Lactose motility agar was steamed for 10 minutes and cooled immediately prior to use. Inoculated tubes were incubated at 35°C for 24 hours. Non-motile, lactose positive cultures in LM agar, ex- hibiting typical microscopic morphology, were considered to be C. Perfringens* . Counts were presented as 50/g when one colony was confirmed from 0.1 ml of a 1:10 dilution. When no colonies were observed, counts were presented as£l00/g

7.

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since the lowest dilution per gram was 1:100.

RESULTS AND DISCUSSION

The results of chemical analysis of herbs and spices are shown in Tables 2 through 8. These tables show the chemical analysis of herbs and spices sub- mitted by four (4) CONUS installations from each of the four Services and industry in comparsion with specification requirements.

Table 2 Bay Leaves - Generally, most samples met the specification require- ments of EE-S-631G for % total ash, acid Insoluble ash and moisture; however, almost 60% of the samples had a volatile oil level less than the specification requirement. This condition may have resulted from the procurement, initially, of low quality products, or products which deteriorated during storage due to improper packaging or both of these factors.

Table 3 Garlic- Specification requirements of JJJ-O-1866 for acid insoluble ash was exceeded by 1/2 of the samples and moisture was exceeded by over 1/3 of the samples. This condition may be due to the same reasons stated for bay leaves. High acid insoluble ash may have been due to contamination with dirt or sand and high moisture content may have been due to a high moisture initial- ly or poor packaging.

Table 4 Ground Cinnamon- Generally, samples met the specification requirements of EE-S-631G except that the % volatile oil was lower in almost 3/4 of the samples. This condition is again similar to that for bay leaves.

Table 5 Oregano- Oregano met the moisture requirement of EE-S-631G; however, % total ash was higher in over a third of the samples, % acid insoluble ash was higher in almost 2/5 of the samples and % volatile oil was lower in over 2/3 of the samples. The greater ash and acid insoluble ash content indicate the product may be heavily contaminated with extraneous materials such as stones, sand, fibers, etc. The lower volatile oil content in the samples is probable due to the same assumptions cited for bay leaves.

Table 6 Chili Powder- Over 3/4 of the chili powder samples met the specification requirements of MIL-C-3394C for % total ash. However, none of the samples met the minimum ASTA color requirement. The ASTA color requirement in the specification is "not less than 70 ASTA extractable color." The requirement for 70 ASTA color may be attained through proper blending of California chili pepper (Anaheim variety of Capsicum annum). A high level of California chili pepper is necessary in order to meet the color requirements, i.e., "bright deep red" for peppers and "reddish brown color" in the finished chili powder. Below 70 ASTA color value means that possible an insufficient quantity of California chili pepper was used in the blend or that the color had deteriorated during storage or both factors were occurring.

Table 7 Mustard, Ground - Generally, 2/3 of the samples met the specification requirements of EE-S-631G for total ash. The product essentially conformed to the specification.

8.

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Table 8 Cayenne Pepper, ground - The cayenne pepper is no longer called cayenne but is called red pepper. This change was made through ASTA. Furthermore, the Scoville heat range was revised to incorporate both red pepper and cayenne pepper using 30M to 55M Units as specified in EE-S-631H. The results of Scoville Pungency Ratings of red pepper as compared with the control samples submitted by industry sources are shown in Table 8. The Scoville rating and the moisture met the specification requirements of EE-S-631G. EE-S-^631G requires 30M Units for red pepper and 45M Units for cayenne pepper and a moisture content of not more than 10.0 percent.

Table 9 Profile Panel Evaluation of Spices and Herbs - The results of the pro- file panel evaluation of herbs and spices are shown in Table 9. In this . evaluation, the individual herbs and spices were compared with control samples submitted by a leading condiment manufacturer. The evaluations of these items are as follows:

Bay Leaves - Out of 15 samples submitted, 10 or 2/3 of the samples were rated weak. Garlic Powder - Out of 13 samples submitted, 3 or approximately 1/4 of the samples were rated weak. Cinnamon, Ground - Out of 16 samples of ground cinnamon submitted, 8 or 1/2 or the samples were rated weak. Oregano Powder - Out of 14 samples of oregano powder, 3 or about 1/4 of the samples were rated weak. Chili Powder - Out of 16 samples of chili powder, 4 or about 1/4 of the samp- les were rated weak. Mustard Powder - Out of 14 samples, 7 or 1/2 were judged as weak. Red Pepper - Out of 13 samples, 5 were judges as weak and 1 sample had no pepper flavor. - .

The results of the packaging evaluations are shown in Tables 10-16. The poly- styrene cup with poly-friction cap used for bay leaves was thought to be too permeable to the atmosphere and thus unsatisfactory. The slip cover fiber- board carton was found to be an unsatisfactory package, see Tables 10 and 12; however, ground mustard packed in a carton with a slip cover was rated as satisfactory by the profile panels, see Table 15, although the panel reported that the ground mustard had a musty after taste. It is possible that this after-taste may have been absorbed from contaminants of the carton. The glass- ine lined paper bag folded for closure used in packaging of the bay leaves (Table 10), was found to be satisfactory if the bay leaves are used within a short period of time. This was substantiated by the profile evaluation and per cent volatile oil. It is possible that this type of packaging material may be suit- able for a leafy type of product such as bay leaves or oregano where the vola- tile components are entrapped within the leafy material» The friction top can and dredge top can were found to be borderline packages as shown in Tables 10 through 16. This was substantiated by profile tests. Data for some spices indicate that the friction top can offers a slight advantage. None of these cans were hermetically sealed. The glass bottle with plastic screw cap was found to be adequate as a packaging media, although a few samples were rated

9,

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unsatisfactory by profile panels as shown in Tables 10-16. It was noted that the glass bottle with plastic cap appears superior to the dredge top and friction top cans; however, it does not prevent moisture transfer because of the loose fit of its plastic screw cap.

Lastly, the: hermetically-sealed can is one of the best barriers possible for herbs and spices, although it requires some sort of reclosure device. It was used as shown in Tables 11, 12, 13, 14, 15,and 16.

Microbiological testing of the herbs and spices was conducted as shown in Tables 17-23. Aerobic plate counts ranged from 100 to 9.1 x 10° per gram de- pending on which spice was being tested. Yeast and mold counts ranged from 100 to 6.7 x 10 per gram. Coliforms were found in cinnamon, garlic powder, and red pepper. No fecal coliforms were found in any of the spices (30/g low- est dilution). Coagulase positive staphylococci were found in one sample of oregano.

C. perfringens was found in oregano, cinnamon, red pepper, and bay leaves, in a range of 50 to 2850 per gram. Oregano had the highest incidence of C. perfrin_- gens.

What is the public health significance ofthese findings? According to Peppier 12, aerobic plate counts rarely bear any relation to the safety of the spice. On the other hand high plate counts in spices do not always signify that food spoil- age will result from their use since usually only small amounts are used to flavor foods. Notwithstanding,low aerobic plate counts do not assure safety or that foodispoilage will not occur. The presence of coliform organisms does not necessarily mean that the spices have been contaminated by pathogens. In this regard many spices reportedly contain natural inhibitors which not only control the development of pathogens in the spice itself but in some instances help con- trol other organisms In foods to which the spice is added. Inhibition; however, was not demonstrated in vitro using E.; coli and S^. aureus_ as test microbes. Spices, heavily contaminated with yeast and! mold, may release lipases which can substantially reduce the storage life of the product to which they are added.

10.

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CONCLUSION

The results of this study indicate that the Services are being supplied herbs and spices sometimes of marginal or poor quality. This may be due to initial "marginal" quality, or to poor packaging, or to a combination of both. If these items continue to be procured on a "local" basis and not inspected, they should be obtained only from reputable sources to Insure initial good quality. In order to preserve initial quality, they should be procured in protective packag- ing, (hermetically sealed containers), along with a polyethylene snap-on-lid for reclosure after opening. Dating of packages would assist inventory control and encourage more rapid turnover. Storage at cool temperatures will help maintain quality, also. Ideally, procurements of sufficiently large quantities on a centralized basis in order to justify adequate inspection and verification testing (as in the can with black pepper) would provide the best solution for assuring that spices and herbs of suitable potency are supplied to the Military Services.

11.

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REFERENCES

1. 1968. Chili Powder Seasoning, Military Specification MIL-C-3394.

2« 1974. Onions and Garlic, Dehydrated/Federal Specification JJJ-Q-1866.

3« 1971. Spices, Ground and Whole, Federal Specification, EE-S-631.

4« 1968. Official Analytical Methods of the American Spice Trade Association, Second Edition.

5. Carl, J. F. 1957. The Profile Method of Flavor Analysis. Advances in Food Research 7, 1-40.

6. Association of Official Analytical Chemists, 1970. Official Methods of Analysis of the Association of Official Analytical Chemists. 11th Edition, Association of Official Analytical Chemists, Washington, D..C.

7. Heidebaugh, N.D., D.B. Rowley, E.M. Powers, C.T. Bourland and J.L. McQueen. 1973, Microbiological Testing of Skylab Foods. Appl. Microbiol. 25 (1): 55-61.

8. Frazier, W.C. 1967. Food Microbiology. McGraw and Hill Book Co., New York.

9. Longree, Rarla. 1967. Quantity Food Sanitation. Interscience Publishers, A Division of John Wiley and Sons, New York.

10. Powers, E.M., C, Ay, H.M. El-Bisi and D.B. Rowley. 1971. Bacteriology of Dehydrated Space Foods.

11. Shahidi, S.A. and A.R. Ferguson. 1971. New Quantative, Quantitative and Confirmatory Media for Rapid Analysis of Food for Clostridium perfringens. .'*:% A^lQOMiÖtoobiol. 21:500-506. c s —

12. Peppier, H.J. 1975. Microorganisms in Spices: What they mean, and how to reduce them. Canners Packer 144 (2) 34-35.

12,

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Table 1. Location of Continental_ynited„States Bases Subrni tti ng_ Samp 1 es

Navy Bases

a. Navy Subsistence Office, Puget Sound, Bremerton, WA. b. Navy Subsistence Office, Norfolk, VA c. Naval Supply Center, San Diego, CA d. Naval Construction Battalion, Gulfport, MS

Marine Corps Bases

e. Marine Corps Base, Camp Pendelton, CA f. Marine Corps Base, Camp Lejeune, NC' g. Marine Corps Air Station, Yuma, AZ h. Marine Corps Base, Quantico, VA

Air Force Bases

i. 62nd Air Base Group (SVF), McChord, AFB, HA j. Chanute Air Force Base, IL k. Bergstrom Air Force Base, TX 1. Dover Air Force Base, DE

Army Bases

m. Fort George Meade, MD n. Hq.j Fort Sam Houston, TX o. Fort Bragg, NC p. Fort Ord, CA

Industry

ET-Ethylene treated NT-Non-ethylene treated

13.

Page 18: UNITED STATES ARMY NATICK, MASSACHUSETTS 01760 … · and spices: total ash, acid insoluble ash, volatile oil (expressed as ml/100g), non-volatile ether extract, ASTA color value,

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