tissue engineering 2015-11-10 location: arvid …...nov 10, 2015 biomaterials research centre’s...
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"Tissue Engineering" 2015-11-10
Location: Arvid Carlsson lecture hall, Medicinaregatan 3, Gothenburg
08.30-09.00
Registration
09.00-09.10
Welcoming address Carina B. Johansson, PhD, Prof. Inst. of Odontology, Dep. of Prosthodontics/Dental Materials Science, Sahlgrenska Academy, University of Gothenburg. Chair of Biomaterials Research Centre (BRC).
09.10-9.55 “Engineering tissues and organs: The next frontier in regenerative medicine” Suchitra Holgersson, Prof. of Transplantation Biology Laboratory for Transplantation and Regenerative Medicine Sahlgrenska Science Park, Gothenburg, Sweden
09.55-10.15 ”NovaHep – Engineering Individualized Blood Vessels” Raimund Strehl, PhD, CTO, NovaHep AB, Gothenburg, Sweden.
10.15-10.45 Coffee
10.45-11.30 11.30-11.50 11.50-13.00 13.00-13.40 13.40-14.00 14.00-14.45 14.45-15.15 15.15-15.35 15.35-16.00
“Testing Implant Materials using Patient-specific Engineered Bone” Giuseppe Maria de Peppo, BSc, MSc, PhD. Principal Investigator The New York Stem Cell Foundation Research Institute, New York, USA. “The Area of Advance Materials Science - Materials in the year of light” Alexandar Matic, Prof. and Director Chalmers Area of Advance Materials Science, Chalmers University of Technology, Gothenburg, Sweden. LUNCH “Healthy and disease skin models for research and screening purposes” Abdoelwaheb El Ghalbzouri, PhD, Ass. Prof. LUMC skin research lab, Leiden, The Netherlands. “Regulatory use of in vitro skin equivalents” Kristina Fant, Research scientist, SP Technical Research Institute of Sweden, Borås, Sweden “Material and Clinical Working Scientists” -”Hand in Hand”- for Development of Scaffolds Stimulating Stem Cell Growth and Bone Regeneration Anna Finne Wistrand, Ass Prof. Dep. of Fibre and Polymer Technology, School of Chemical Science and Engineering, KTH Royal Institute of Technology, Stockholm, Sweden. Kamal Mustafa, Prof. Dep. of Clinical dentistry, Center for Clinical Dental Research, University of Bergen, Norway. Coffee “Biomaterials in the year of light – Emerging microscopy techniques for 3D imaging of tissue-mimicking milieus” Annika Enejder, Prof. Dep. of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden. “How to educate the next generation of tissue engineering researchers” Patric Wallin, PhD cand. Applied Physics and Engineering Education Research, Chalmers University of Technology, Gothenburg, Sweden. Discussion & End of the Day Become a member: www.brc.org.gu.se
Biomaterials Research Centre Nov 10, 2015
Biomaterials Research Centre’s Annual Day 2015 "Tissue Engineering"
Presentation of speakers
Dr. Suchitra Sumitran-Holgersson earned her Doctor of Medical Sciences degree
in Clinical Immunology at the Karolinska Institutet in 1991, and became an associate
Professor in 2000. In 2008, she joined the Sahlgrenska hospital at the Gothenburg
University as the Professor of Transplantation Biology. Her research interests include
understanding mechanisms of allograft rejections, stem cell engraftment and tissue-
engineering of organs with stem cells. She has contributed to more than 80 articles in
peer-reviewed journals and 15 book chapters and has seven scientific patents to her
credit.
Raimund Strehl, PhD, Chief Technology Officer for NovaHep, holds a degree in
cell biology from the University of Regensburg, Germany. He has an academic
background within cell culture development and tissue engineering as well as an
industrial background in the field of human pluripotent stem cell development and
application. Raimund has worked over ten years at Cellartis, heading product
development and manufacturing in the role of Chief Technology Officer, responsible
for collaborations with major pharmaceutical and biotech companies.
Dr. de Peppo received a BSc Degree in Biotechnology at La Sapienza University in
Rome and a MSc degree in Medical Biotechnology at Bicocca University in Milan,
with a thesis in biomaterial engineering from Politecnico di Milano. Following an
advanced course in Bioinformatics in Valencia, in 2007, he was awarded a Marie
Curie fellowship at the Department of Biomaterials at the University of Gothenburg
in Sweden, where he received an international PhD in Tissue Engineering. In 2013 he
was honored the Best Prize for his doctoral studies and the Argos Hippium Award for
his professional achievements abroad. After completing his PhD, Dr. de Peppo was
awarded a postdoctoral fellowship at the New York Stem Cell Foundation Research
Institute, where is now leading the Bone Engineering and Regeneration group. His
major research goal is to engineer patient-specific vascularized bone grafts for large
and complex skeletal reconstructions using an osteoinductive scaffold – perfusion
bioreactor model of bone development. Other research directions include
manufacturing of biomaterial scaffolds, testing of implantable biomaterials using
tissue-engineered products, and stem cell therapy using pluripotent stem cells.
Aleksandar holds a Senior Research Position from the Swedish Research council
directed towards Soft Matter Physics and is also director for Chalmers Area of
Advance Materials Science.
The research spans from fundamental investigations of glass formation, gelation
and colloidal aggregation to applied projects on new electrolytes for Li-batteries
and fuel cells.
Suchitra Sumitran-
Holgersson MSC, PhD
Prof. of Transplantation
Biology Laboratory for
Transplantation and
Regenerative Medicine
Sahlgrenska Science Park,
Göteborg
Giuseppe Maria de
Peppo, BSc, MSc, PhD Principal Investigator
The New York Stem Cell
Foundation
Research Institute
www.nyscf.org
Raimund Strehl PhD, CTO, NovaHep
AB, Gothenburg,
Sweden.
Alexandar Matic Prof. and Director
Chalmers Area of
Advance Materials
Science, Chalmers
University of
Technology,
Gothenburg, Sweden.
Biomaterials Research Centre Nov 10, 2015
Biomaterials Research Centre’s Annual Day 2015 "Tissue Engineering"
Presentation of speakers
Abdelouahab El Ghalbzouri (1973) studied Biotechnology and Biochemistry
(BSc, 1994-1997) in Etten-Leur, the Netherlands and Molecular and Cellular
Biology (MSc, 1998) at the Medical University Paris VI in Paris (France). In
1999, he started his PhD at the Department of Dermatology of the Leiden
University Medical Center (LUMC). During his PhD, he optimized the generation
of reconstructed human skin models and extensively studied their use for tissue
engineering, research, and clinical purposes. Abdoel continued his work on
human skin models as a post-doc (2004-2006) and became senior scientist
(universitair docent, UD) at the Department of Dermatology (LUMC). Currently,
he is leading the research group ‘disease skin models’, in which various skin
diseases (e.g. eczema, squamous cell carcinoma) and skin conditions (e.g. wound
healing, skin aging) are mimicked in vitro to better understand the
physiopathology of the skin. In 2008, this research group was awarded the
‘Alternative to animal testing award’ from the Dutch Society for Animal
Protection and the Netherlands Centre for Alternatives to Animal Use. In 2009,
the group received the ‘Pearl project award’ for mimicking skin cancer in vitro
from the Netherlands Organization for Health Research and Development
(ZonMw). In 2010 he participated in the NGI venture challenge where he
presented his spin-off company Biomimiq. Biomimiq offers services with its
unique and customizable in vitro human skin models representing both healthy
and diseased human skin, ascertaining the company as one of the leaders in a
large and rapidly growing market. In January 2013, Biomimiq was incorporated
as a separate unit within the Tissue Engineering company Aeon Astron Europe
B.V. (Leiden, The Netherlands), where it continued its services on skin models.
As founder, he has a scientific advisory role within Biomimiq-AAE
Dr Kristina Fant is a research scientist at SP Technical Research Institute of
Sweden since 2011. She has a PhD in Physical Chemistry from Chalmers
University of Technology. Her area of expertise is in vitro safety testing and the
development of alternative test methods, i.e. methods not involving animal
experiments. She is developing and implementing test methods to be run in
compliance with Good Laboratory Practice principles. The SP cell culture
facilities are currently the only Swedish lab to offer GLP approved safety testing
to external customers, and Sweden’s representative in the EU-NETVAL network
of qualified laboratories that can participate in method validation.
Anna Finne Wistrand has a background in polymer synthesis. Her research
interests focus today on tissue engineering and the translational, interdisciplinary
field of material science and biology. This is an area which requires an enhanced
understanding of polymer synthesis, physico-chemical characteristics,
characterization and structure-property relationships at both molecular and
nanoscale level. A general aim for the research is to design and fabricate complex
three-dimensional constructs that attract multiple cell types in a predetermined
manner and permit development of extracellular matrices in a well defined way.
Anna Finne Wistrand finished her PhD 2003 at KTH Royal Institute of
Technology, Fibre and polymer Technology. She has after that spent some time as
visiting scientist in the group of (1) Prof. Virgil Percec, Department of Chemistry,
University of Pennsylvania (2) Prof. Y. Ito, Nanomedical Engineering, RIKEN.
In addition, she has had positions at Akzo Nobel (Casco Adhesive), PP Polymer
and she is right now active at Novus Scientific.
Abdelouahab El
Ghalbzouri, PhD, Ass. Prof. LUMC
skin research lab,
Leiden, The Netherlands.
Anna Finne Wistrand Ass.Prof. Dep.of Fibre and
Polymer Technology,
School of Chemical
Science and Engineering,
KTH Royal Institute of
Technology, Sweden
Kristina Fant Research scientist,
SP Technical
Research Institute
of Sweden, Borås,
Sweden
Biomaterials Research Centre Nov 10, 2015
Biomaterials Research Centre’s Annual Day 2015 "Tissue Engineering"
Presentation of speakers
Kamal Mustafa is a professor at the University of Bergen, Norway and leader of
Tissue Engineering Group. He is also an active member of Bergen Stem Cell
Consortium. His research activity has been tremendously increased during the last
3 years focusing on the development of a tool box for bone regeneration according
to the concept of tissue engineering. The research group is producing innovative
research in the field of biomaterials, cell biology, regenerative medicine and
translation research. The main research area is addressed to develop translational
approaches for regenerative therapies of different skeletal defects. The group
involves a vital collaboration between Cell Biologists, Material Scientists,
Engineers and Clinicians aiming to develop an appropriate scaffold for bone tissue
engineering. Currently, he is sponsoring and leading a maxillofacial clinical trial
which is among the few in Europe with Advanced Therapy Medicinal Products
using MSC and biomaterials.
Kamal Mustafa received his PhD from Karolinska Institute in 2001. Then, he had
a postdoc period at three different institutes; Karolinska Institute, University of
Oslo and University of Bergen. He worked also as visiting scientist at The Nordic
Institute for Dental Materials Oslo (NIOM), University of North Carolina, Chapel
Hill, USA and University of Malmö, Sweden.
Annika Enejder is Professor in Molecular Microscopy, Department of Biology and
Biological Engineering, Chalmers University of Technology. She started her
academic career as a PhD student in the Division of Atomic Physics, Lund
University, where she studied the fundamentals of laser light interaction with cells
and biomolecules (Prof. Sune Svanberg). After a few years in the industry she
returned to academia through a VR postdoc scholarship and spent four years at the
Spectroscopy Lab, MIT, USA and the Ludwig-Maximilian University, Munich
(Raman and CARS microscopy). She has devoted all her scientific career to
interdisciplinary research, integrating advanced physical technologies to the benefit
of the biosciences. She is currently a part-time Marie Curie Fellow at Stanford
University in Prof. Sarah Heilshorn’s Biomaterials Group and has transferred her
unique technologies of recombinant protein engineering of ECM-mimicking proteins
for tissue-mimicking environments. The Marie Curie Fellowship has also offered
insights in the latest technologies in Biopolymer- and Neuro-sciences, Stem cell and
cancer biology as well as neurodegenerative diseases. On the European arena,
Annika has been the chair of the European Network for nonlinear Raman microscopy
since 2006 involving ~50 EU groups and is the coordinator of a FP7 Marie Curie
Innovative Training Network FINON, for the development of novel technology for
nano-scale nonlinear microscopy.
Patric Wallin is a PhD candidate in Bioscience with a specialization in
Educational Science. He is working in the Division of Biological Physics at
Chalmers University of Technology, and is associated with the Division of
Engineering Education Research (EER). He has tutored projects in a master
level course on Tissue Engineering at Chalmers for several years, and has
studied how students learn about Tissue engineering with different educational
research approaches. Patric’s research interests in the education research field
are focused around undergraduate research experiences, communities of practice
and situated cognition. He is particular interested in the progress and
development processes students experience in these learning situations. Presentation of speakers
Annika Enejder, Prof. Dep. of Biology
and Biological
Engineering,
Chalmers University
of Technology,
Gothenburg, Sweden.
Patric Wallin, PhD candidate
Applied Physics and
Engineering Education
Research, Chalmers
University of Technology
Kamal Mustafa
Prof. Tissue Engineering
Research Group - Leader
Dep. of Clinical Dentistry-
Center for Clinical Dental
Research University of
Bergen, Norway
Biomaterials Research Centre´s Annual Day 2015-11-10 “Tissue Engineering”
Abstracts
“Engineering tissues and organs: The next frontier in regenerative medicine”
Suchitra Holgersson, Prof. Dep. of Surgery, Sahlgrenska University Hospital, Gothenburg, Sweden
Different pathological end-stage diseases have few effective therapeutic options apart from a whole organ
transplant that, however, often meets with a serious shortage of donor organs. To solve these clinical issues
we are using a novel tissue engineering (TE) approach in which a whole donor organ is decellularized to
obtain a complex 3D-biomatrix-scaffold maintaining the intrinsic vascular network, that is subsequently
recellularized with recipient's autologous organ-specific differentiated cells or/and stem cells, to build a
potentially functional biological substitute.
“NovaHep – Engineering Individualized Blood Vessels”
Raimund Strehl, PhD, CTO, NovaHep AB, Gothenburg, Sweden
NovaHep focuses on the commercialization of a unique technology to engineer individualized blood vessels
using a decellularized tissue scaffold and the patient's own stem cells. The engineered blood vessels can be
used to replace non-functioning blood vessels in patients with different types of vascular disease.
“Testing Implant Materials using Patient-specific Engineered Bone”
Giuseppe Maria de Peppo, BSc, MSc, PhD. Principal Investigator, The New York Stem Cell
Foundation Research Institute, New York, USA.
Millions of people worldwide require orthopedic treatments to reconstruct defects caused by traumatic
accidents and disease. Metal implants (such as titanium and its alloys) can form a stable bond with bone, an
interaction that highly depends on the surface characteristics of the implant material. Osseointegration is
traditionally studied using experimental assays and animal studies. However, available in vitro methods fail
to provide insights regarding the complex tissue response to the materials in a 3D setting under conditions
mimicking the native physiological environment. On the other hand, in vivo studies are time and resource
intensive, and often irrelevant due to interspecies differences in tissue quality and metabolism. In order to
bridge the existing gap in implantology research, we have engineered patient-specific bone grafts from
human induced pluripotent stem cells, and used these as research platforms to study the cellular response to
the implant material, the strength of interaction of the implant with the engineered bone, and the quality of
the bone-implant interface. In addition to allow development and screening of implant surfaces promoting
enhanced osseointegration, this platform could represent the first important step towards the generation of
personalized treatments without the need for animal testing.
Biomaterials Research Centre´s Annual Day 2015-11-10 “Tissue Engineering”
Abstracts
“Healthy and disease skin models for research and screening purposes”
Dr. A. El Ghalbzouri , PhD, Ass. Prof. LUMC skin research lab, Leiden, The Netherlands
Tissue engineering involves the use of living cells to develop biological substitutes for tissue replacements.
Next to the treatment of acute and chronic wounds, tissue engineering offers new opportunities as a tool for
the industry. For example, reconstructed human skin equivalents (HSEs) are representative models of human
skin and widely used for screening purposes, clinical applications and research purposes. Since in vitro HSEs
recapitulate most of the in vivo characteristics of human skin, they contribute to the replacement of animal
experimentation. Human epidermal skin models are mostly used to predict the safety of ingredients used in
various industries, while full thickness HSEs are more often applied for research and tissue engineering
purposes. These HSEs are all generated with skin tissue obtained from healthy donors. In dermatology, there
is unmet clinical need to develop therapies for a large number of skin diseases, including skin cancer,
psoriasis and eczema. Currently there are no such off-the shelf in vitro models available that harbour specific
diseased characteristics to screen and validate novel targets, and test the effects of potential new therapies in
vitro. Therefore, a highly active research area is emerging by combining tissue engineering principles and
knowledge from developmental biology to establish complex three-dimensional in vitro disease model
systems representing various skin conditions and diseases, including recessive epidermolysis bullosa
simplex, cutaneous squamous cell carcinoma and melanoma, wound healing and skin aging. These HSEs are
an excellent tool to gain more insight into the mechanisms of various pathological conditions and contribute
to the development of appropriate therapies and novel therapeutics.
“Regulatory use of in vitro skin equivalents”
Kristina Fant, Research scientist, SP Technical Research Institute of Sweden, Borås, Sweden
Progress in the development of test systems based on in vitro tissue engineering in the last decades have
contributed to a significant decrease in animal use for industrial risk and safety assessment of products and
ingredients. This is also reflecting extensive research efforts sparked e.g. by stricter legislation foremost
within the EU, where the REACH Regulation stipulates that animal testing should only be undertaken as the
last resort when no other methods are available, and the Cosmetics Regulation forbids all animal testing on
finished cosmetic products and their ingredients since 2013 regardless of the availability of alternative
methods. Also for medical devices, recent changes in the ISO 10993 series put an increasing emphasis on the
use of cell-based methods before animal tests both for ethical reasons and to provide improved predictivity
and reproducibility.
Reconstruction of human epidermis was first described in the scientific literature over thirty years ago and
has since then reached a stage where the model is organotypic, i.e. it reflects the histological appearance and
the lipid composition of normal epidermis. Thanks to dedicated research efforts in industry and in EU-funded
projects, already from the start aiming specifically for possible future validation of methods and regulatory
acceptance, RhE technology has finally been adopted as stand-alone methods in OECD test guidelines:
OECD TG 431 for skin corrosion (2004) and OECD TG 439 for skin irritation testing of chemicals (2009).
However, the methods are not yet validated to also cover testing of medical devices where the irritating
potential is usually much lower. An ISO 10993/TC194-organized validation study is underway and some of
the considerations in this study will be discussed.
Biomaterials Research Centre´s Annual Day 2015-11-10 “Tissue Engineering”
Abstracts
“Material and Clinical Working Scientists -”Hand in Hand”- for Development of Scaffolds
Stimulating Stem Cell Growth and Bone Regeneration”
A Finne-Wistrand1, K. Mustafa
2
1 Department of Fibre and Polymer Technology, School of Chemical Science and Engineering, KTH Royal Institute of Technology,
Sweden. 2Department of Clinical dentistry, Center for Clinical Dental Research, University of Bergen, Norway
In tissue engineering, the regeneration process is affected by the scaffold microstructure as well as its initial biological
and biomechanical properties. There are in general two ways to influence the cell-material interactions, optimize the
polymer microstructure and optimize the scaffold design. Our group has strategically evaluated the cell-material
interactions and has today a broad knowledge of the influence from functionality, hydrophilicity and crystallinity as
well as the scaffold design, porosity, pore structure and mechanical properties. The different strategies presented here
are from projects where we are working with scaffold-based therapies aimed at successful engineering of bone
constructs for clinical use.
The copolymer design is often used for tuning cell-material interactions. We know that even small differences in
polymer microstructure influence the material properties and thereby also the cell-material interaction. As an example
of this, poly(LLA-co-DXO) scaffolds enhance the attachment and differentiation of human osteoblast-like cells in vitro
compared to poly(LLA-co-CL).1
In these copolymer scaffolds pore sizes of >90 was shown to induce better cellular and
tissue responses where blood vessels can be recruited. It was also found that endothelial microvascular networks can be
generated through the polymer scaffolds and maintained in vivo influencing the osteogenic potential of the tissue
engineered construct.
Another way to tune the mechanical properties and the hydrophilicity is to add surfactants. In vitro and in vivo data
generated recently, indicated that tuning hydrophilicity of copolymer scaffolds with 3% of Tween 80 promotes the
proliferation of bone marrow derived stem cells (MSC) and induce bone formation (Un published data). Furthermore,
cellular responses was shown to be improved by modifying the surface of the poly(LLA-co-CL) scaffolds with nano-
diamond particles (n-DP). n-DP modification significantly increased cell attachment and differentiation of MSC on
poly(LLA-co-CL) scaffolds in vitro and enhanced bone formation in vivo.2,3
Another strategy that we are using to tune material-cell interactions is to functionalize the polymers and immobilize
growth factors. We have, for example, synthesized epoxy-functionalized polyesters4 and covalently immobilized
heparin and we have compared covalently attached and physisorbed BMP2 to the scaffold.5
The physisorption of BMP2
onto nDP modified copolymer scaffolds appears to hold great promise compared to growth factors adsorbed solely onto
a polymer. A low dose of BMP2 physisorbed onto scaffolds modified with nanodiamond particles was shown to be
bioactive for bone regeneration. Obtaining bone after 4 weeks in vivo as demonstrated by histology and µCT analyses
suggests accelerated bone regeneration in relation to the modified copolymer scaffolds.
The concept of using a combination of a biomaterial and stem cells for bone regeneration has been tested recently in
human. This phase I study was performed in Bergen employing 11 patients required reconstruction of alveolar bone. It
is among few clinical studies in Europe with Advanced Therapy Medicinal Products. The study indicate that the use of
biomaterials combined with MSC in the applied protocol for augmentation of the atrophied mandibular ridge have
results comparable to the gold standard; autologous bone transplantation.
A close collaboration between material scientists, cell biologists and clinicians is important for finding optimal
parameters and this talk will describe how we systematically have worked with this during the years.
REFERENCES: 1 S. Dånmark, A. Finne-Wistrand, M. Wendel, et al (2010) J Bioact Compat Polym 25:207-223.2 Z. Xing, T. O. Pedersen, X. Wu, et al (2013) Tissue
engineering 19:1783-1791. 3 Y. Sun, A. Finne-Wistrand, T. Waag, et al (2015) Macromol. Mater. Eng. 300: 436–447 4J. Undin, A. Finne-Wistrand,
A.-C. Albertsson et al (2013) Biomacromolecules 14: 2095-2102. 5 S. Suliman, Z. Xing, XJ. Wu, et al (2015) J. Control Release, 197, 148-157.
ACKNOWLEDGEMENTS:
The authors acknowledge the European Union 7th Frame Program, VascuBone (project number 242175), The Swedish Research Council (Dnr 621-2013-3764), The Norwegian Research Council (17734/V50, 180383/V4
Biomaterials Research Centre´s Annual Day 2015-11-10 “Tissue Engineering”
Abstracts
“Biomaterials in the year of light – Emerging microscopy techniques for 3D imaging of
tissue-mimicking milieus”
Annika Enejder, Prof. Dep. of Biology and Biological Engineering, Chalmers University of Technology,
Gothenburg, Sweden.
We develop an emerging category of laser-based microscopy techniques for 3D visualization of biopolymers (native
and bioengineered fibrous proteins, carbohydrates, membrane lipids etc.) in living cells and extra-cellular mimicking
matrices/surfaces. This allows us to monitor the dynamic and dual interaction between cells and ECM
components/different surface chemistries under close to native conditions without any sample preparations. Instead of
attaching a bulky fluorophore to the target molecules, their inherent vibrations are resonantly enhanced by frequency-
matched electromagnetic waves from pico-second short laser pulses and then detected as blue-shifted scattered light;
Coherent Anti-Stokes Raman Scattering (CARS) and/or Second Harmonic Generation (SHG) signals. The group hosts
one of the most technically well-equipped laboratories for non-linear
microscopy in Europe and the single one in Scandinavia, allowing for
simultaneous CARS, Raman, 2-photon fluorescence, Second and Third
Harmonic Generation (SHG & THG) microscopy. Unique images of adipocyte-
derived stem cells in tissue-mimicking recombinant protein-engineered
scaffolds (collaboration with the Heilshorn group, Stanford University) and
fibroblast cells on lipid coated surfaces (Julie Gold, Chalmers) will be shown
without any sample preparation, sectioning or labeling.
CARS microscopy image at the carbon-hydrogen vibration 2930 cm-1
characteristic for
proteins, showing the distribution of fibers in an electrospun scaffold of elastin-like
proteins synthesized by recombinant protein-engineering by the Heilshorn group,
Stanford University. The image covers 35x35x10 µm.
“How to educate the next generation of tissue engineering researchers”
Patric Wallin, PhD cand. Applied Physics and Engineering Education Research, Chalmers University of
Technology, Gothenburg, Sweden.
Tissue engineering is very research intensive both in academia and industry, and graduates that are going to work in
the field are required to have the ability to advance the scientific frontier. Therefore, tissue engineering education
needs to reflect the nature of the discipline and establish a strong link between research and teaching. In this talk, I
will present a framework that can be used to establish different approaches to link research and teaching.
In the second part, I will focus on my own case study to describe how teaching and research is linked in a master’s
course on tissue engineering. A central component of the course is an authentic research project that the students carry
out in smaller groups and in collaboration with faculty. I will discuss how the students experience learning in this kind
of discovery-oriented environment based on data from reflective writing and interviews. From the data three themes
related to the students’ learning experiences were identified: learning to navigate the field, learning to do real research
and learning to work with others. Overall, the students strongly valued learning in a discovery-oriented environment
and three aspects of the course contributed to much of its success: taking a holistic approach to linking teaching and
research, engaging students in the whole inquiry process, and situating authentic problems in an authentic physical and
social context.