TISSUE BIOREPOSITORYAND

PATHOLOGY SERVICES

TISSUE PROCUREMENT

• Routinely collect tumor tissue, adjacent normal tissue, buffy coat, plasma, newly diagnosed and pre-transplant hematologic samples

• Also collect urine, saliva, and biopsies for particular projects

• All tissue samples receive quality control analysis before distribution

• Site specific committees comprised of surgeons and researchers must approve request for samples that are in low inventory. Ensures that valued samples are given to important projects

DONOR EVENTS 2011*event could be any combination of tissue and blood samples

JANUARY 67 FEBRUARY 68 MARCH 88 APRIL 78 MAY 75 JUNE 89 JULY 72 AUGUST 102

Breast 4 Bladder 2 Bladder 5 Bladder 5 Bladder 2 Bladder 4 Bladder 2 Bladder 4

CNS 7 Breast 3 Breast 5 CNS 9 Breast 2 Breast 6 Breast 5 Breast 10

Endocrine 1 Cervical 1 CNS 13 Colon 2 CNS 4 Cervix 2 CNS 8 CNS 10

Gastric 1 CNS 3 Colon 2 Endocrine 4 Colon 1 CNS 4 Endocrine 1 Colon 2

Intestine 2 Endocrine 1 Endocrine 5 Esophagus 1 Endocrine 4 Colon 3 Esophagus 1 Endocrine 2

Liver 3 Gastric 1 Gastric 2 Gastric 1 Gastric 1 Endocrine 5 Gastric 1 Gastric 1

HN 17 Liver 5 Intestines 1 Liver 1 Liver 3 Esophagus 1 HN 14 HN 18

Leukemia 1 HN 14 Liver 6 HN 15 HN 19 Liver 5 Leukemia 7 Leukemia 12

Lung 7 Leukemia 8 HN 9 Leukemia 8 Leukemia 11 HN 13 Liver 4 Liver 7

Pancreas 2 Lung 7 Leukemia 8 Lung 3 Lung 3 Leukemia 9 Lung 4 Lung 1

Prostate 11 Ovary 5 Lung 5 Other 1 Ovary 3 Lung 7 Other 2 Other 5

Other 1 Pancreas 1 Ovary 5 Ovary 2 Pancreas 3 Other 3 Ovary 1 Ovary 5

Rectum 2 Prostate 8 Pancreas 4 Pancreas 3 Prostate 7 Ovary 1 Pancreas 3 Pancreas 2

Renal 4 Renal 7 Prostate 6 Prostate 9 Rectum 1 Pancreas 3 Prostate 6 Prostate 8

Uterus 4 Skin 1 Renal 3 Rectum 2 Renal 4 Prostate 5 Rectum 1 Rectum 1

Uterus 1 Sin 3 Renal 5 Skin 3 Rectum 3 Renal 6 Renal 9

Soft Tissue 1 Skin 1 Soft Tissue 1 Renal 8 Uterus 6 Sin 1

Uterus 5 Soft Tissue 1 Uterus 3 Skin 3 Uterus 4

Uterus 5 Testes 1 Uterus 4

Vulva 1 Gastric 1

Collections: 2010 vs. 2011

Month 2010 2011

January 40 67

February 41 68

March 31 88

April 52 78

May 64 75

June 51 89

July 60 72

August 63 102

Each month, we have increased our tissue collection over what it was in the previous year

Pathology Services

• Tissue fixation, processing, and embedding• Frozen and FFPE tissue sectioning• H&E staining• Immunohistochemistry• RNA isolation and BioAnalyzer• LCM• Tissue MicrroArray• *In the process of offering special stains *• Other requests …

Laser Capture Microdissection

Cryostat & Microtome

PixCell IIXT

Bioanalyzer

Extraction & QC

RiboAmp TM& Paradise TM

Amplification

Captured cells After captureH&E section

Cell Selection

Extracted RNA

Amplified

Unamplified

qPCR analysis

BIOANALYZER

• Determine sample quality before performing downstream applications

• Faster than gels – up to 12 samples in 30 min• Minimal sample consumption; 1 μL• Ready-to-use reagent kits• Chip preparation in less than 5 min

TISSUE MICRO ARRAY

• Services provided: – Custom TMA construction– TMA slide sectioning– Immunohistochemistry and in situ hybridization on TMA slides– TMA pathology imaging scoring– Support for grant applications by providing LCM budgets and support

letters

Contact InformationKiki Gibbs

Phone: 843-792-8165E-mail: gibbski@musc.edu

Location: room 421 Bioengineering Building