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Centre for Forensic Science www.forensics.uts.edu.au Assoc. Prof. Peter Gunn [email protected]
UTS CRICOS PROVIDER CODE: 00099F
UTS: Centre for Forensic Science
Vision and Mission
• Vision: – Crime reduction, reduction of fear of crime, crime
solving and security in general are major objectives for society.
• Mission: – To provide and advance a modern and validated
body of scientific knowledge to address questions that are fundamental to the concepts of national security and public safety, including: intelligence, law enforcement and justice.
UTS: Centre for Forensic Science
Flagship Research Programmes
Fingerprint Detection
and Identification
Forensic DNA in the
21st Century
In-Field Detection of Explosives and Drugs
Forensic Taphonomy
Forensic Intelligence
Drugs & Toxicology
UTS: Centre for Forensic Science
Our recent & current collaborative research
• VicPol / ThermoFisher – current • Australian Museum – echidnas • AFP – hyperspectral imaging • Royal Botanic Gardens • Gene Health Technologies (Ningbo, China) • Beijing Institute of Genomics (Chinese Academy
of Sciences) • NSWPF – fired cartridge cases
UTS: Centre for Forensic Science
Technical capabilities
• Applied Biosystems 3500 Genetic Analyser – Globablfiler®, Powerplex 21®, Compass® forensic
applications
• ThermoFisher IonTorrent® massively parallel sequencing platform
UTS: Centre for Forensic Science
Development of Genetic Markers for the Short Beaked Echidna
Australian Centre for Wildlife Genomics, Australian Museum, 6 College St, Sydney, NSW Centre for Forensic Science, University of Technology Sydney, NSW, Australia
NGS – Illumina &/or Ion Torrent mitochondria
UTS: Centre for Forensic Science
• Echidna blood samples are being provided through collaborating Zoo and Aquarium Association institutions, as well as tissue samples from the Australian Museum collections
• Both captive bred and wild born echidnas being tested to get a range of information
• DNA extracted and then ddRAD next generation sequencing techniques being used to develop Single Nucleotide Polymorphism (SNP) markers
• Mitochondrial DNA full sequence analysis also to be applied • Sequences will be analysed and pedigree developed. More echidnas
from zoos in other countries will be tested to obtain as much information as possible about echidna populations
Development of Genetic Markers for the Short Beaked Echidna
UTS: Centre for Forensic Science
Alexandra Summerell1,2, Greta Frankham1, Peter Gunn2, Rebecca Johnson1
1. Australian Centre for Wildlife Genomics, Australian Museum, 6 College St, Sydney, NSW 2. Centre for Forensic Science, University of Technology Sydney, NSW, Australia
Development of Genetic Markers for the Short Beaked Echidna
UTS: Centre for Forensic Science
The reproducibility, accuracy & reliability of Low Copy Number DNA profiling using multiple sampling of profiles Armstrong, G. & Gunn P.R.
UTS: Centre for Forensic Science
Validation of the Compass® DNA identification kit
• In conjunction with – Health Gene Technologies, Ningbo, China – Beijing Institute of Genomics – Total of 34 genetic markers
UTS: Centre for Forensic Science
Conclusion
“The Consensus Method produced accurate DNA profiles in a majority of tests. 95.45% of the consensus profiles produced were accurate in comparison to the reference profile. When individual alleles were assessed it was found that 99.79% were accurate to the comparison…………….”
UTS: Centre for Forensic Science
Can the pollen content of sinus cavities infer the time/place of death?
The Retrieval and Identification of Palynomorphs from Nasal Cavities and Their Role in Determining a Location and Season
of Death
Alexandra Summerell (Bachelor of Forensic Biology in Biomedical Science)
11242587
2014
UTS: Centre for Forensic Science
Can the pollen content of sinus cavities infer the time/place of death?
UTS: Centre for Forensic Science
Application of NGS to examination of microbial content of maggot masses in decomposition
Demonstrates the clustering of skin, buccal larval mass, and larval mass samples separated by time. A definite clustering (similarity) of Day 4 (blue) samples is seen. Days 6 (orange) and 8 (green) also demonstrate strong similarity within the groups, as well as Day 10 (purple).
UTS: Centre for Forensic Science
UTS CRICOS PROVIDER CODE: 00099F UTS:SCIENCE
Identification of single nucleotide polymorphisms (SNPs) involved
in the determination of facial morphology
science.uts.edu.au
Presented by: Dr. Mark Barash
UTS: Centre for Forensic Science
African Caucasian Asian
American Indian Aboriginal Polynesian
Cranial diversity in different human populations
UTS: Centre for Forensic Science
Africans (AA)
Caucasians (NAC) Asians
Normal facial morphology variation in human populations
UTS: Centre for Forensic Science
Extending this work
• Uts – Human Research Ethics Committee – Approval for ~ 1,000 samples
UTS: Centre for Forensic Science
Extending this work
• Uts – Human Research Ethics Committee – Approval for ~ 1,000 samples
• Physical data to be collected includes:
– 3-D images for measurement of facial parameters – Fingerprints – Dental images - CT – DNA
• WGS or selected targets • Mitochondrial DNA sequencing
UTS: Centre for Forensic Science
Current personnel
• Assoc Prof Peter Gunn ([email protected])
• Dr Mark Barash ([email protected])
• 4 x Ph.D students • 4 x masters students • 3 x honours students