thermo scientific orion versa star™ ph/ise/temperature ......thermo scientific orion versa star...

Thermo Scientific Orion VERSA STAR™ pH/ISE/Temperature Module

Reference Guide

Ross and the COIL trade dress are trademarks of Thermo Fisher Scientific, Inc. and its subsidiaries.

AQUAfast, AQUASensors, BOD AutoEZ, ionplus, KNIpHE, LogR, No Cal, ORION, perpHect, PerpHecT, pHISA, pHuture, Pure Water, Sage, ROSS, ROSS Ultra, Sure-Flow, Titrator PLUS, and TURBO2 are registered trademarks of Thermo Fisher Scientific, Inc. and its subsidiaries.

A+, All in One, Aplus, AUTO-BAR, AUTO-CAL, Auto-ID, AUTO-READ, AUTO-STIR, Auto-Test, AutoTration, CISA, digital LogR, DuraProbe, EZ Startup, ISEasy, Low Maintenance Triode, Minimum Stir Requirement, MSR, NISS, Optimum Results, Orion Dual Star, Orion Star, SAOB, SMART AVERAGING, SMART STABILITY, Star LogR, Star Navigator 21, Stat Face, Triode are trademarks of Thermo Fisher Scientific, Inc. and its subsidiaries.

Guaranteed Success and The Technical Edge are service marks of Thermo Fisher Scientific, Inc. and its subsidiaries.

© 2011 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific, Inc. and its subsidiaries.

The specifications, descriptions, drawings, ordering information and part numbers within this document are subject to change without notice.

This publication supersedes all previous publications on this subject.

Thermo Scientific Orion VERSA STAR™ pH/ISE/Temperature Module

Table of Contents

Chapter 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1

Chapter 2 Module Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

Module Connections and Inputs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3Module Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4Display Information From Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

Chapter 3 Setup Menus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

Setup Menus for Primary Measurements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5Read Type Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6Isopotential Value . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7Linear Regression Information . . . . . . . . . . . . . . . . . . . . . . . .7Low Level Stability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7

Setup Menu for Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

Chapter 4 pH, RmV and ORP Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

pH Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9RmV Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9ORP Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

Chapter 5 ISE Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

Chapter 6 Temperature Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Chapter 7 Measurement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

pH/mV/RmV/ORP Measurement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15ISE Measurement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

Chapter 8 Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

Chapter 9 Incremental Techniques . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

Known Addition and Subtraction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19Single Known Addition . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20Double Known Addition . . . . . . . . . . . . . . . . . . . . . . . . . . . .20Single Known Subtraction . . . . . . . . . . . . . . . . . . . . . . . . . .21Double Known Subtraction . . . . . . . . . . . . . . . . . . . . . . . . .21

Analyte Addition and Subtraction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22To Access Incremental Techniques . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

Chapter 10 Data Storage and Review . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

Data Storage Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23Measurement Read Type . . . . . . . . . . . . . . . . . . . . . . . . . . .23Datalog, Computer and Printer Export Settings . . . . . . . . . .23

Datalog Review . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23Calibration Review . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

Chapter 11 Customer Services . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Troubleshooting Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25Assistance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25Warranty and Registration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25WEEE Compliance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25Declaration of Conformity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26pH/ISE/Temperature Module Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

Appendix Advanced Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

Automatic pH Buffer Recognition Feature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33Testing a pH Electrode for Automatic Buffer Recognition . 33

pH Electrode Condition Icon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34Multipoint Calibration Curve Feature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35Automatic Blank (Non-Linear) Correction Feature . . . . . . . . . . . . . . . . . . . . . . . 36Determining an Isopotential Value . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

1Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module

Chapter 1 Introduction

Thank you for your purchase of the Thermo Scientific Orion VERSA STAR™ pH/ISE/Temperature Module.

This module is for use with the Thermo Scientific Orion VERSA STAR Advanced Electrochemistry Benchtop Meter.

Some key features of this module include:

• pHrangefrom-2to20withresolutionoptionsof0.001,0.01and0.1

• ISErangefrom0to19999withresolutionofuptofoursignificantdigits

• mVandRmVrangesfrom±2000mV

• Automaticandmanualdataloggingoptions

• Upto6-pointpHcalibrationwithautomaticrecognitionforUSA/NISTandDINbuffers

• Upto6-pointISEcalibrationwithadjustableISOpointcapabilities

• TheabilitytofixpH/ISEcalibrationerrorswithoutacompleterecalibrationthankstocalibrationediting

• AdvancedISEfeaturessuchasauto-blank,timedendpoint,linearpoint-to-point,non-linearselectable

auto-blank,low-concentrationrangestability,ISEIDandincrementaltechniques

Pleasereadthisreferenceguidethoroughly.Anyuseoutsideoftheseinstructionsmayinvalidateyourwarrantyand

causepermanentdamagetothemeter.

This page intentionally left blank.


Chapter 2 Module Overview

Toconnectthemodulestothemeterandforspecificinformationregardingthemeteroutputs,pleaseseethe

OrionVERSASTARReferenceGuide.TheReferenceGuideisontheCDincludedwiththemeterandalsoavailable

at www.thermoscientific.com/water.

Module Connections and Inputs

TheOrionVERSASTARmetercanacceptOrioncombinationISEelectrodesaswellasseparatereferenceandsensing

half-cellelectrodes.ThisallowsforawiderISEmeasurementoffering.Half-cellelectrodesseparatetheionssensing

fromthereferenceelectrode.Fordifficultsamples,theflexibilityofusingalternatefillsolutionsmaybeanadvantage

to researchers.

1. PreparethepHorISEelectrodeandATCprobeforuseasinstructedintheelectrodeuserguides.

The pin tip reference connector is on the lowest panel of the pH/ISE module.

2. PlacethepHorISE,ATCprobeandOrionStarstirrerprobe(cat.no.096019,optional)intothe

electrode arm.

3. AttachthepHorISEBNCandATCprobetothepH/ISEmetermodule.LowerISEandATCinto

solutionforconditioningifrequired.Ifusingthestirrerprobe,attachprobetothemeter,placeintothe

electrodearmandassigntheprobetothechannelinthesetupmenuforinstrumentsettings.

4. Preparefreshstandardsdailyforcalibration.ForISEtesting,standardsshouldbeatleastonedecade

(10times)apart.Thestandardsshouldexceedtheexpectedminimumandmaximumsamplerange.

Serialdilutionisrecommendedusingcalibratedpipettestoaccuratelypreparecalibrationstandards

from a stock solution.

5. Gatherreagents,rinsewater,andothersupplies.

ForISEtesting,ifISAwillbeaddedtothesample,itneedstobeaddedtoallthestandardspriorto

calibrationtoensureaconsistentdilutionfactor.

pH/ISE Module

8pinmini-DINforATC

BNCconnectionwithshortingcap

Reference pin socket

4 Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module

Module Maintenance

Forroutinemaintenance,dustandwipethemodulewithadampcloth.Ifnecessary,warmwaterormildwater-based

detergentcanbeused.Maintenancecanbeperformedonadaily,weeklyormonthlybasis,asrequiredbyoperating

environment.Immediatelyremoveanyspilledsubstanceusingthepropercleaningprocedureforthatspilltype.

Display Information from Module

ExampleISEMeasurementScreen–SingleChannel

Dateandtime

Primaryreading

Calibrationinformation

CorrespondingmV value

Method

Theinformationonthedisplaycanbecustomized.Enterthesetupmenutomakechanges.1.PressSetup.2.UsethearrowkeystohighlightInst.Settings, press (f3/select) key.3.UsethearrowkeytohighlightDisplayView. Press (f3/select) key.4. Follow the onscreen prompts to choose what you would like to see on the measurement screen.


Chapter 3 Setup Menus

Setup Menus for Primary Measurements

1. PressSetup (f3) to access the Main Setup Screen.

2. UsethearrowkeytoscrollacrosstothemodulestoISEchannelyouwishtouseand

press Select (f3).

3. Scroll to across to the mode menu and press Select (f3).

ISE Setup Menu (Default values are in bold in the table below)ElectrodeSerialNumber InputElectrode Type SeetablebelowSampleID Off,Manual,AutoIncrementSignificantDigits 1,2,3,4digitsStability Smart,Fast,Medium,SlowAveraging Off,Automatic Smart Read Type AutoRead,Timed,ContinuousMeasureUnit ppm,Molar,mg/L,%,ppb,NoneIsopotential Value Off,OnLinearRegression Off,OnBlank Correction Off,OnLowLevelStability Off,OnAlarm** Limit,CalDue,SetPoint

**Limitandsetpointalarmdefaultsetto‘off’. Calduealarmsetto12hours.

pH Setup Menu (Default values are in bold in the table below)

ElectrodeID InputSampleID Off,Manual,AutoIncrementResolution 0.1,0.01,0 .001Stability Smart,Fast,Medium,SlowAveraging Off,Automatic SmartRead Type AutoRead,Timed,ContinuousBuffer Group USA,DIN,CustomIsopotential Value Off,On 7 .000LinearRegression Off,OnAlarm** Limit,CalDue,SetPoint

**Limitandsetpointalarmdefaultsetto‘off’. Calduealarmsetto12hours.


4. ForISEtesting,selecttheISEelectrodebeingusedinsetup.Ifyourelectrodeisnotlisted,

usethe“X”electrodewiththevalence(+1,+2,-1,or-2)matchingyourelectrodefor

accurate results.

Read Type Information

Therearethreemainreadtypesavailableasshowninthesetupmenus.

• Auto-Read – Themeterwilldisplaythemeasurementasitstabilizesandlockandholdthe

measurementwhenitisstable(TheARiconwilllockonthescreen.)Oncethemeasurementislocked,

themeterwillautomaticallyexportthemeasurementtothedatalog,ifthedatalogisenabledin

thesetupmenu,andtoaprinterorcomputer,ifaprinterorcomputerisconnectedtothemeterand

enabledinthesetupmenu.Totakeanewmeasurement,pressmeasure (esc)/1.

• Timed – Themeterwillreadanddisplayameasurementatthesettimeintervalthatisprogrammed

bytheoperator.Therearetwooptions:

1. Bychecking/selectingthe"Interval"box,atthesettimeinterval,themeterwillautomatically

exportthemeasurementtothedatalog,ifthedatalogisenabledinthesetupmenu,andto

aprinterorcomputer,ifaprinterorcomputerisconnectedtothemeterandenabledinthe

setupmenu.Ifselected,enterthetimeintervalvalueinahours:minutes:secondsformatusing

the numeric keypad and press f3 (select) key.

2. Ifthe"Interval"boxisunchecked/notselected,asingletimedmeasurementwillbetaken.

Themeterwillapplythesamesavingandexportingconditionsaswiththesettimeintervalto

recordandexportdata,butlockafteronetimedintervalhasexpired.Pressmeasure (esc)/1 to

resetthecountdownclockandtakeanewreading.

ISE TypesAg+ I- X- CO2 REDOX(ORP) Cl2 S-2 ClO4 O2

Cd+2 NH3 BF-4 K+ X-2 Cu+2 X+ F- SCN-CN- Pb+2 Cl- NO3- Br- NOx Ca+2 Na+ X+2

ORP, RmV and mV Setup Menus (Default values are in bold in the table below)

ElectrodeID InputSampleID Off,Manual,AutoIncrementResolution 1.0,0 .1Stability Smart,Fast,Medium,SlowAveraging Off,Automatic SmartRead Type AutoRead,Timed,ContinuousAlarm** Limit,CalDue*,SetPoint

*Only for ORP and RmV modes. **Limitandsetpointalarmdefaultsetto‘off’. Calduealarmsetto12hours.


• Continuous – The meter will continuously measure and update the display. This read type is useful

whenperforminganexperimentthatrequirescontinuousmeasurementstobetaken.Pressthe

log/print/0printkeytoexportthemeasurementtothedatalog,ifthedatalogisenabledinthesetup

menu,andtoaprinterorcomputer,ifaprinterorcomputerisconnectedtothemeterandenabledin

the setup.

Isopotential Value

Theslopeofallelectrodeschangeswithtemperature,basedontheNerstequation.Theslopecurvesatthedifferent

temperaturesintersectattheisopotentialvalue,whichistheconcentrationatwhichthepotentialoftheelectrodedoes

notvarywithtemperature.ForglasspHelectrodes,thisvalueis7.00.Forotherionselectiveelectrodes(ISEs),ifthe

isopotentialvalueisknownorcanbemeasuredexperimentally,adjustingthisvaluemakestemperaturecompensation

foranISEpossible.Temperaturecompensationcanbeperformedforoneandtwopointcalibrations.

Linear Regression Information

Thedefaultisoffwhichusesthetraditional,segmentedapproach.Thesegmentedapproachisthemostaccurateand

precise.MoreinformationcanbefoundintheappendixsectionofthisreferenceguideundertheMultipointCalibration

Curve Feature.

Selecting“on”forthisoptionresultsintheuseofasinglebestfitlineforthecalibrationcurve,andcanbebeneficialif

pHmeasurementsvarygreatly.

Low Level Stability

ThisfeatureadjuststhetimingforthecalibrationpointsforlowerlevelISEstandards.

Setup Menu for Temperature

AllofthetemperaturemeasurementsettingsarefoundinTemperatureSetupmenuforeachmeasurement.

*Thisnumberwillchangetomatchthesetupmenuchannel.

Temperatureprobescanbesetupinthemeasurementmodeofthesampleyouaremeasuring.Ch-1ATCallowsthe

temperatureprobetobeusedinmorethanonechannelfortemperature-compensatedmeasurements,suchaswhen

apHelectrodeanda2-cellconductivitycellareinthesamemeasurementsample.TemperatureisacriticalpHand

conductivitymeasurementasthemeasurementvaluesrequireatemperaturecorrectiontodeterminethereference

value,often25ºCintheAmericaand20ºCinEurope.

Temperature Unit ºCorºFselectionTemperature Input and Type Ch-1ATC,Ch-2ATC,Ch-3ATC,Ch-4ATC,MTC

Temperature Cal Ch-1*ATConlyorAllATCallowsallATCstobe calibratedatonce


Chapter 4 pH, RmV and ORP Calibration

pH Calibration

UptosixpHbufferscanbeusedforcalibration.Thiscanbeacombinationofautomatically-recognizedUSA/NISTor

DINbuffervaluesandcustombuffers.AlwaysusefreshpHbuffersandselectbuffersthatbracketthesamplepHand

areatleastonepHunitapart.PreparethepHelectrodeaccordingtotheinstructionsintheelectrodeuserguide.

1. Pressf1 (Cal)toperformapHcalibration.

2. Followscreenpromptsforcalibration.Usefreshbuffers,rinseelectrodewithDIwaterbeforeeach

reading,blotwithalintfreetissue.

3. USAandDINbuffersetsareautomaticallyrecognizedbasedofftheselectioninthesetupmenu,

andthemeterallowscustombufferstobeusedforupto6calibrationpoints.Custombufferoption

prompts for the nominal values and stores data.

4. Duringcalibration,pointscanbeeditedorretestedpriortoacceptance.

5. Informationforthelastcalibrationcanbedisplayedonthemainmeasurementscreen.Refertothis

referenceguide,Chapter2ModuleOverview,DisplayInformationsectionformoreinformation.

RmV Calibration

OnestandardcanbeusedforrelativemVcalibration.Alwaysusefreshstandard.Preparetheelectrodeaccordingto

theinstructionsintheelectrodeuserguide.

1. Pressf1 (Cal) toentercalibrationmode.

2. Followthescreenpromptsforcalibration.

3. Thecalibrationcanbesavedorprintedwhencomplete.




ORP Calibration

ThemVreadingofanORPstandardisdependentontheORPelectrodereferencesystem,referencematerial,filling

solutionandORPstandardtemperature.AnORPelectrodecanbeautomaticallycalibratedtoread420mVrelativeto

theStandardHydrogenElectrode(EH)at25°CwhentheOrionORPstandard(cat.no.967901or96791),OrionORP

electrode(cat.no.9678BNWP,9778BNWP,9180BNMDor9179BNMD)andOrion4MKClfillingsolution(cat.no.

900011)or4MKClgel(inelectrodewithcat.no.9179BNMD)areused.TherawmVreadingofanelectrodewiththe

sameconditionsis220mVat25°C.

OnestandardcanbeusedforORPcalibration.Alwaysusefreshstandard.Preparetheelectrodeaccordingtothe

instructionsintheelectrodeuserguide.

1. Pressf1 (Cal)toentercalibrationmode.

2. Followthescreenpromptsforcalibration.

3. Thecalibrationcanbesavedorprintedwhencomplete.




Chapter 5 ISE Calibration

OnetosixstandardscanbeusedforISEcalibration.Ifmorethanonestandardisused,startwiththelowest

concentrationfirst.Alwaysusefreshstandards.Selectstandardsthatbracketthesampleconcentrationandarea

decadeapart.Preparetheionselectiveelectrodeaccordingtotheelectrodeuseguide.

1. Presschannel key until ISE channel is displayed. Press Cal (f1).Followthescreenpromptsstarting

with the lowest concentration.

2. PressEdit (f3)tothecorrectconcentrationvalueofastandardifitisnotautomaticallyrecognized.

3. Afterthefirstdatapointhasbeenaccepted,pressAccept (2) tocontinuecalibration.

The meter then offers options of Esc (f1)toleavecalibration,Next (f2)measurenextcalibration

point,orCal Done (f3).

4. TheoptionsafterCalDoneispressedarePrint (f1),Cal Edit (f2) or Slope Edit (f3).

5. TheCalibrationreporttableisdisplayedwiththeconcentration,mVreading,andTemperatureand

averageslopewhichisupdatedwitheachsuccessivepointmeasured.

6. Fromthisscreenthenavigationoptionsare Print (f1),Cal Edit (f2) or Slope Edit (f3).Printexports

datatoprinterorcomputer,Caleditallowsconcentrationtobecorrectedpriortothecompletionof

thecalibrationbyDelete (f1) or ReMeasure (f3).

7. PressingtheSlopeEditchangesthescreentitletoSinglePointCal–SlopeEdit.Thisallowsthe

currentslopelinetobeupdatedwiththepresentelectrodeperformance.

8. Press Cal Done (f1)whencompleted,pressviewgraph,savethecalibrationinCalLog.


Chapter 6 Temperature Calibration

TheATCtemperaturedisplayhasarelativeaccuracyof±0.1°C.ATCprobes(bothseparateandthosebuiltinto

electrodes)havevaryingtemperatureaccuracies,usually±0.5°Cto±2°C.Use this function only if necessary.

Sincethetemperatureoffsetcalculatedduringthecalibrationisappliedtoallfuturetemperaturemeasurements,

recalibrateifadifferentATCprobeisused.Fortemperaturecalibration,theprobeneedstobeconnectedtothemeter

andthecalibrationsolutionshouldhaveaknown,stabletemperature.ItisrecommendedthattwoNIST-traceable

thermometersbeusedtomeasureandverifysolutiontemperature.

1. Pressf3 (setup) key.

2. Usethearrowkeystohighlightthechannelthatthetemperatureprobeisconnectedto.

Press f3 (select).

3. UsethearrowkeystohighlightTemperatureandpressf3 (select).

4. UsethearrowkeystohighlighttheappropriateTemperatureCaloptionandpressf3 (select).

5. Follow the onscreen prompts.


Chapter 7 Measurement

pH/mV/RmV/ORP Measurement

Afterasuccessfulcalibration,thesamplescanbetested.

1. Rinsetheelectrodewithdeionizedwater.Blotdrywithlint-freetissue.

2. Placeelectrode/ATCprobeinfirstsampleandstirgently.

3. IfthemeterisinAutoReadmode(meterdefault)pressmeasure (esc) (1) key. If the meter is in

continuousortimedreadmode,themeterwillimmediatelystarttakingreadings.

4. IfinAutoReadmodeanddatalogisenabled(default),thereadingwillautomatically

bestoredwhenthe“AR”appears.Ifincontinuousreadmodeanddatalogisenabled,

press log/print (0) keytostoreintothemeter’smemory.

Note: Toenableordisabledatalogging,presssetupandselectInst.Settings.

ISE Measurement

1. Preparethesampleasinstructedintheelectrodeuserguide.

2. FollowdirectionscarefullyonISAandothersampleadditions.

3. Rinsetheprobe(s)andplaceintothesample.IfthemeterisinAutoReadmode(meterdefault)press

measure (esc) (1)key.Ifthemeterisincontinuousortimedreadmode,

themeterwillimmediatelystarttakingreadings.

4. IfinAutoReadmodeanddatalogisenabled(default),thereadingwillautomaticallybestoredwhen

the“AR”appears.Ifincontinuousreadmodeanddatalogisenabled,presslog/print (0) key to store

intothemeter’smemory.

Note:Toenableordisabledatalogging,presssetupandselectInst.Settings.


Chapter 8 Methods

Themeterstoresuptotenmethodsperchannelwiththelastcalibrationdatastoredforeachmethod.

Usethechannelsetuptoaccessandmodifymethods.


2. Usethearrowkeystohighlighttheappropriatechannel.Pressf3 (select).

3. UsethearrowkeystohighlightMethodandpressf3 (select).

4. Themethodscanbeloaded,copied,savedandeditedbyfollowingtheonscreenprompts.


Chapter 9 Incremental Techniques

Known Addition and Subtraction

TheOrionVERSASTARmeterhaspresetmethodsforsingleknownaddition,singleknownsubtraction,doubleknown

additionanddoubleknownsubtraction.

• Whenperformingincrementaltechniques,thesampleconcentrationmustbeknownwithinan

orderofmagnitudesotheincrementmaybecorrectlychosenandthesampleandstandardmustbe

volumetrically determined.

• Itisveryimportantthatanycomplexingagentispresentinexcess(atleast50to100times)ornot

atall.Theratiooffreetocomplexedionsmustremainconstantovertheaddition.Anindicationthat

acomplexingagentispresent,butnotingreatenoughquantitytomaintainaconstantfreeionto

complexedionratio,isanabnormallyhighorlowslope.Ifthisisasuspectedproblem,addanexcess

amountofcomplexingordecomplexingagenttotheoriginalsolutioninthebeaker.

• Electrodeinterferencesshouldbeataminimum,sincetheeffectoftheinterferencemightchange

astheconcentrationoftheionofinterestchanges.Thissituationmayalsoresultinhighorlow

electrodeslopes.Consulttheelectrodeuserguideforinformationonreducingoreliminatingsome

electrode interferences.

• Knownadditionisausefulmethodformeasuringdilutesamples,occasionalsamplesorsamplesthat

containanexcessofcomplexingagent.Knownadditioncanalsobeusedtoverifytheresultsofa

direct measurement.

• Knownsubtractionisusefulwhenmeasuringionsforwhichstablestandardsdonotexist.Known

subtractioncanalsobeusedasaquickversionofatitration.Whenperformingaknownsubtraction,

itisnecessarytoknowthestoichiometricratiobetweenthestandardandsample.

• Doubleincrementaltechniquescalculatetheelectrodeslopeandsampleconcentration

simultaneously.Theelectrodeslopeisdetermineddirectlyinthesample,whichresultsingreater

accuracyforsampleswithcomplexmatricesandgreateranalysisspeed.


Single Known Addition

A standard of known concentration is added to a sample of unknown concentration. The total concentration should

approximatelydoubleupontheadditionofthestandard,thereforesampleconcentrationshouldbeknownwithinan

orderofmagnitude.Refertothefollowingtabletochoosethecorrectstandardconcentrationandvolumeofaddition.

• Refertotheappropriateelectrodeuserguideforinstructionsforpreparingtheelectrode,required

solutions(ionicstrengthadjusters,standards,etc)andanyspecialrequirements.Determinethe

electrodeslopeasdirectedintheelectrodeuserguide.

• Ensurethattheelectrode,ATCprobeandreferenceelectrode(ifapplicable)areconnectedtothe

meter inputs that correspond with the selected channel.

Double Known Addition

Formostdoubleknownadditionanalysis,itisrecommendedthat:

• Thesamplevolumeshouldbe100mL.

• Theconcentrationofthestandardaddedshouldbe100timestheexpectedsampleconcentration.

• Calculatethevolumeofstandardrequiredtohalvetheinitialsampleconcentrationanddivideby

six.Fivesixthsofthevolumewillbeaddedinthefirstadditionandtheremainderinthesecond.If

available,consultaprintoutfromapreviousanalysisofasimilarsample.Note:theproposedvaluesfor

constantmVwillbesmallerthanthoserecommendedforknownaddition.Thisisanefforttominimize

mechanceofover"subtracting"thesample(overshootingtheendpoint).

Refertotheappropriateelectrodeuserguideforinstructionsforpreparingtheelectrode,requiredsolutions

(ionicstrengthadjusters,standards,etc)andanyspecialrequirements.

Ensurethattheelectrode,ATCprobeandreferenceelectrode(ifapplicable)areconnectedtothemeterinputsthat

correspond with the selected channel.

Amount of standard to be added per 100 mL of sample

Standard concentration compared to expected sample concentration

1mL 100timesmoreconcentrated5mL 20timesmoreconcentrated10mL 10timesmoreconcentrated


Single Known Subtraction

Inknownsubtraction,eachadditionofstandardsubtractsanincrementofthespeciestobemeasured.Enterthe

correctreactionratioofthespeciestobemeasuredwiththestandardtobeadded.Forexample,asulfide(S-2)sample

istobesubtractedbytheadditionofsilver(Ag+).Twosilverionsarerequiredtosubtracteachsulfideionandthe

reactionratiois0.5.

Sampleconcentrationshouldbeknownwithinanorderofmagnitudeandthetotalsampleconcentrationshould

beapproximatelyhalveduponadditionofthestandard.Refertothefollowingtabletochoosethecorrectstandard

concentration and volume of addition.

Refertotheappropriateelectrodeuserguideforinstructionsforpreparingtheelectrode,requiredsolutions(ionic

strengthadjusters,standards,etc)andanyspecialrequirements.Determinetheelectrodeslopeasdirectedinthe

electrodeuserguide.



Double Known Subtraction

Ensurethatthesubtractionabilityofthechosenstandardconcentrationandvolumeaddeddoesnotexceedthe

concentration of sample.

Formostdoubleknownsubtractionanalysis,itisrecommendedthat:

• Thesamplevolumeshouldbe100mL.

• Theconcentrationofthestandardaddedshouldbe50timestheexpectedsampleconcentration.

• Calculatethevolumeofstandardrequiredtohalvetheinitialsampleconcentrationanddivideby

six.Fivesixthsofthevolumewillbeaddedinthefirstadditionandtheremainderinthesecond.If

available,consultaprintoutfromapreviousanalysisofasimilarsample.Note:theproposedvaluesfor

constantmVwillbesmallerthanthoserecommendedforknownaddition.Thisisanefforttominimize

mechanceofover"subtracting"thesample(overshootingtheendpoint).

Refertotheappropriateelectrodeuserguideforinstructionsforpreparingtheelectrode,requiredsolutions(ionic

strengthadjusters,standards,etc)andanyspecialrequirements.



Amount of standard to be added per 100 mL of sample

Standard concentration compared to expected sample concentration

1mL 50timesmoreconcentrated5mL 10timesmoreconcentrated10mL 5 times more concentrated


Analyte Addition and Subtraction

InAnalyteAddition,thefirstmeasurementisinaknownstandardsolutioncontainingthesametypeofanalytein

interest as the sample. The second measurement follows the addition of the unknown solution. The concentration of

the unknown is then calculated.

InAnalyteSubtraction,thefirstmeasurementusesknownstandardsolutioncontainingthetypeofionsthattheISE

canmeasureandthatwillcomplexwiththeanalyteininterestintheunknownsample.Thesecondmeasurement

follows the addition of the unknown sample that reduces the concentration of the ions in the measurement solution.

The concentration of the analyte in interest in the unknown is then calculated.

• Isopotentialvalueneedstobeknownandentered.

• Blankcorrectionisnotapplied.

• Theslopevalueat25°Cneedstobeknownandentered.

To Access Incremental Techniques

1. SetthemeasurementmodetoISE.


3. UsethearrowkeystohighlighttheISEchannel.Pressf3 (select).

4. UsethearrowkeystohighlightIncrementalTechniquesandpressf3 (select).

5. Follow the onscreen prompts.


Chapter 10 Data Storage and Review

Data Storage Settings

Measurement Read Type

The measurement read type determines when the meter sends measurements to outputs as selected in the setup menu

and when properly connected to the meter. These are set individually for each channel. Measurement Read Type is set

perchannelinthesetupmenu.Pleasereferchapter3SetupMenusinthisreferenceguideformoreinformation.

Datalog, Computer and Printer Export Settings

OrionVERSASTARMetershavea2000-datalogcapability.Tomakeyourselection:

1. Frommeasurementmode,presssetup/3.

2. PressthearrowkeystohighlightInst.Settings.Presssetup/3.

3. Usethearrowkeys,f2 (page) key and f3 (select) key to:

a. set the communication protocol

b.turnonprinting

c. select the print format

d.turnondatalogging

Formoreinformationonthesesettings,refertotheOrionVERSASTARReferenceGuide,Chapter3InstrumentSettings

toturnonDataLogtoenabledatastorage.

Datalog Review

Thisoptionallowsforstoreddatatobe:

• reviewed

• printedindividually,inagrouporall

• deleted

• analyzedwithgeneralstatisticalinformation

Thedatalogwillreflectascreencaptureofalltheinformationatthetimeofdatalogging.

Toreviewdataloginformation

• Presslog view (9) key

• Pressf3 (select) key

• Followpromptstoreviewinformation


Calibration Review

Thisfeatureallowsforreviewandprintingofeachofthelast10calibrationsforeachparameterofthemeter.

Theinformationwillmatchtheinformationrecordedforthatcalibration.

Toreviewcalibrationinformation

1. Presslog view (9) key.

2. Pressright arrow (6) keytoselectCalLoginformationanddown arrow (8)tohighlightthe

desired parameter.

3. Press f3 (select) key and follow the prompts.


Chapter 11 Customer Services

Troubleshooting Guide

Assistance

Aftertroubleshootingallcomponentsofyourmeasurementsystem,contactTechnicalSupport.WithintheUnitedStatescall1.800.225.1480andoutsidetheUnitedStatescall978.232.6000orfax978.232.6031.InEurope,theMiddleEastandAfrica,contactyourlocalauthorizeddealer.Forthemostcurrentcontactinformation,orthelatestapplicationandtechnicalresourcesforThermoScientificOrionproducts,visitwww.thermoscientific.com/water.

Warranty and Registration

Toregisteryournewmeterandforthemostcurrentwarrantyinformation,visitwww.thermoscientific.com/water.

WEEE Compliance

ThisproductisrequiredtocomplywiththeEuropeanUnion’sWasteElectrical&ElectronicEquipment(WEEE)Directive2002/96/EC.Itismarkedwiththesymbolabove. ThermoFisherScientifichascontractedwithoneormorerecycling/disposalcompaniesineachEUMemberStateandthisproductshouldbedisposedoforrecycledthroughthem.Furtherinformationoncompliancewiththesedirectives,therecyclersinyourcountry,andinformationonThermoScientificOrionproductsthatmayassistthedetectionofsubstancessubjecttotheRoHSDirectiveareavailableatwww.thermoscientific.com.

Error Recommended Actions

Measurement is flashing9999andoverrangeorunderrange.

Measurementisoutsidetheallowablemeasurementrange.Checkelectrodeconnectionandsettingsinthesetupmenu.Cleantheelectrodeaccordingtotheelectrodeuserguideandrecalibratetheelectrodewithnewbuffersorstandards. Iftheerrorcontinues,performthemeterselftestinthediagnosticsmenu.

The measurement freezesandwill notchange.

ThemeterisinAutoReadmode.(ARiconappearssolidonthedisplay.)Press measure (esc)/1keytotakeanewreadingorgotosetuptochangethereadtype.


Declaration of Conformity

Manufacturer: Thermo Fisher Scientific Inc .

Address: AyerRajahCrescent Blk55#04-16/24 Singapore139949 Singapore

Hereby declares that the following products:Orion VERSA STAR Advanced Electrochemistry Benchtop Meters with Modules are rated 100to240VAC,50/60Hz,0.5A.

Equipment Class:

Measurement,controlandlaboratoryOrionStarA-seriesmetersareEMCClassA

Conforms with the following directives and standards:

EN61326-1:2006 Electromagnetic Compatibility (EMC Directive) Electricalequipmentformeasurement, controlandlaboratoryuse-EMCrequirements

EN61010-1:2001 Safety StandardsUL61010-1:2004 Safetyrequirementsforelectricalequipmentformeasurement,CAN/CSA C22 .2 No . 61010-1-04 controlandlaboratoryuse-generalrequirements

CheowKwangChan QA/RegulatoryManager

PlaceandDateofIssue: 12December,2011 Singapore


pH/ISE Module (VSTAR-ISE) SpecificationspH Range -2.000to20.000

Resolution 0.1,0.01,0.001Relative Accuracy ±0.002CalibrationPoints Upto6CalibrationEditing YesAdjustableISOPoint Yes

mV/RmV Range ±2000.0mVResolution 0.1Relative Accuracy ±0.2mVor±0.05%ofreadingwhicheverisgreaterEH ORP Mode Yes

ISE Range 0to19999Resolution Upto4significantdigitsRelative Accuracy ±0.2mVor±0.05%ofreadingwhicheverisgreaterUnits ppm,M,mg/L,%,ppb,noneCalibrationPoints Upto6CalibrationEditing YesAdjustableISOPoint Yes

CalibrationFeatures Timedendpoint,linearpointtopoint,non-linearselectableauto-blank,lowconcentrationrangestability

IncrementalTechniquesSingleknownaddition&subtraction, doubleknownaddition&subtraction, singleanalyteaddition&subtraction

Advanced Features Displayselectrodetype,temperaturecompensationTemperature Range -5to105°C,23to221ºF

Resolution 0.1Relative Accuracy ±0.1OffsetCalibration 1point

Inputs pH/ISE:BNC,referencepin ATC:8-pinmini-DIN

pH/ISE/Temperature Module Specifications

NOTE:Wereservetherighttomakeimprovements.Specificationssubjecttochangewithoutnotice.


Ordering Information

CML # DescriptionVSTAR00 Orion VERSA STAR Benchtop Meter

-Electrodearmwithredesignedholder -Universalpoweradapter

VSTAR10 Orion VERSA STAR pH Benchtop Meter Set -OrionVERSASTARmeterwithpH/temperaturemodule -Electrodearmwithredesignedholder -Universalpoweradapter

VSTAR12 Orion VERSA STAR pH Benchtop Meter Kit -OrionVERSASTARmeterwithpH/temperaturemodule -8302BNUMDOrionROSSTriodepH/ATCprobe -096019OrionStarstirrerprobe -810199ROSSsolutionkit(475mLeachofpH4,7and10buffers; storagesolution;cleaningsolution;andpHelectrodestoragebottle) -Electrodearmwithredesignedholder -Universalpoweradapter

VSTAR20 Orion VERSA STAR Conductivity Benchtop Meter Set -OrionVERSASTARmeter -OrionVERSASTARconductivity/temperaturemodule -Electrodearmwithredesignedholder -Universalpoweradapter

VSTAR22 Orion VERSA STAR Conductivity Benchtop Meter Kit -OrionVERSASTARmeter -OrionVERSASTARconductivity/temperaturemodule -013005MDOrionDuraProbeconductivitycell,K=0.475 -011007conductivitystandard,1413µS,5x60mL -Electrodearmwithredesignedholder -Universalpoweradapter

VSTAR30 Orion VERSA STAR RDO/Dissolved Oxygen Benchtop Meter Set -OrionVERSASTARmeter -OrionVERSASTARdissolvedoxygen/temperaturemodule -Electrodearmwithredesignedholder -Universalpoweradapter

VSTAR32 Orion VERSA STAR RDO/Dissolved Oxygen Benchtop Meter Kit -OrionVERSASTARmeter -OrionVERSASTARdissolvedoxygen/temperaturemodule -083005MDOrionpolarographicDOprobe -080017calibrationsleeveforDOprobe -080513DOprobemaintenancekit -970802BODfunnel/stirrer -080360BODadapter -Electrodearmwithredesignedholder -Universalpoweradapter


CML # DescriptionVSTAR40A Orion VERSA STAR pH/ISE Benchtop Meter Set

-OrionVERSASTARmeter -OrionVERSASTARpH/ISE/temperaturemodule -Electrodearmwithredesignedholder -Universalpoweradapter

VSTAR40A2 Orion VERSA STAR pH/ISE Benchtop Meter Kit -OrionVERSASTARmeter -OrionVERSASTARpH/ISE/temperaturemodule -8102BNUWPOrionROSSUltrapHelectrode -096019OrionStarstirrerprobe -927007MDATCprobe,stainlesssteel -ROSSsolutionkit(475mLeachofpH4,7and10buffers;storagesolution; cleaningsolution;andpHelectrodestoragebottle) -Electrodearmwithredesignedholder -Universalpoweradapter

VSTAR40B Orion VERSA STAR Benchtop Meter with Two pH/ISE Modules -OrionVERSASTARmeter -TwoOrionVERSASTARpH/ISE/temperaturemodules -Electrodearmwithredesignedholder -Universalpoweradapter

VSTAR40B2 Orion VERSA STAR Benchtop Meter with Two pH/ISE Modules Kit -OrionVERSASTARmeter -TwoOrionVERSASTARpH/ISE/temperaturemodules -8102BNUWPOrionROSSUltrapHelectrode -096019OrionStarstirrerprobe -927007MDATCprobe,stainlesssteel -ROSSsolutionkit(475mLeachofpH4,7and10buffers;storagesolution; cleaningsolution;andpHelectrodestoragebottle) -Twoelectrodearmswithredesignedholders -Universalpoweradapter

VSTAR50 Orion VERSA STAR pH/Conductivity Benchtop Meter Set -OrionVERSASTARmeter -OrionVERSASTARpH/temperaturemodule -OrionVERSASTARconductivity/temperaturemodule -Electrodearmwithredesignedholder -Universalpoweradapter

VSTAR52 Orion VERSA STAR pH/Conductivity Benchtop Meter Kit -OrionVERSASTARmeterwithpH/temperaturemodule -OrionVERSASTARconductivity/temperaturemodule -8157BNUMDOrionROSSUltraTriodepH/ATCprobe -013005MDOrionDuraProbeconductivitycell,K=0.475 -810199ROSSsolutionkit(475mLeachofpH4,7and10buffers; storagesolution;cleaningsolution;andpHelectrodestoragebottle) -011007conductivitystandard,1413µS,5x60mL -Electrodearmwithredesignedholder -Universalpoweradapter


CML # DescriptionVSTAR80 Orion VERSA STAR pH with LogR Benchtop Meter Set

-OrionVERSASTARmeter -OrionVERSASTARpH/temperaturewithLogRtechnologymodule -Electrodearmwithredesignedholder -Universalpoweradapter

VSTAR82 Orion VERSA STAR pH Benchtop Meter Kit -OrionVERSASTARmeter -OrionVERSASTARpH/temperaturewithLogRtechnologymodule -8172BNXOrionROSSSure-FlowpHelectrode -927007MDATCprobe,stainlesssteel -ROSSsolutionkit(475mLeachofpH4,7and10buffers;storagesolution; cleaningsolution;andpHelectrodestoragebottle) -810007ROSSfillsolution,5x60mL -Electrodearmwithredesignedholder -Universalpoweradapter

VSTAR90 Orion VERSA STAR pH/ISE/Conductivity/RDO/Dissolved Oxygen Benchtop Multiparameter Meter Set -OrionVERSASTARmeter -OrionVERSASTARpH/ISE/temperaturemodule -OrionVERSASTARconductivity/temperaturemodule -OrionVERSASTARdissolvedoxygen/temperaturemodule -Electrodearmwithredesignedholder -Universalpoweradapter

VSTAR91 Orion VERSA STAR pH/ISE/Conductivity/RDO/Dissolved Oxygen Benchtop Multiparameter Meter Set -OrionVERSASTARmeter -TwoOrionVERSASTARpH/ISE/temperaturemodules -OrionVERSASTARconductivity/temperaturemodule -OrionVERSASTARdissolvedoxygen/temperaturemodule -Two096019OrionStarstirrerprobes -Twoelectrodearmswithredesignedholders -Universalpoweradapter


CML # DescriptionVSTAR92 Orion VERSA STAR pH/ISE/Conductivity/RDO/Dissolved Oxygen

Benchtop Multiparameter Meter Set -OrionVERSASTARmeter -OrionVERSASTARpH/ISE/temperaturemodule -OrionVERSASTARconductivity/temperaturemodule -OrionVERSASTARdissolvedoxygen/temperaturemodule -8157BNUMDOrionROSSUltraTriodepH/ATCprobe -013005MDOrionDuraProbeconductivitycell,K=0.475 -083005MDOrionpolarographicDOprobe -080017calibrationsleeveforDOprobe -080513DOprobemaintenancekit -970802BODfunnel/stirrer -080360BODadapter -810199ROSSsolutionkit(475mLeachofpH4,7and10buffers; storagesolution;cleaningsolution;andpHelectrodestoragebottle) -011007conductivitystandard,1413µS,5x60mL -Electrodearmwithredesignedholder -Universalpoweradapter

VSTAR93 Orion VERSA STAR pH/ISE/Conductivity/RDO/Dissolved Oxygen Ultimate Benchtop Multiparameter Meter Set -OrionVERSASTARmeter -OrionVERSASTARpH/ISE/temperaturemodule -OrionVERSASTARconductivity/temperaturemodule -OrionVERSASTARdissolvedoxygen/temperaturemodule -8157BNUMDOrionROSSUltraTriodepH/ATCprobe -013005MDOrionDuraProbeconductivitycell,K=0.475 -083005MDOrionpolarographicDOprobe -Two096019OrionStarstirrerprobes -080017calibrationsleeveforDOprobe -080513DOprobemaintenancekit -970802BODfunnel/stirrer -080360BODadapter -810199ROSSsolutionkit(475mLeachofpH4,7and10buffers; storagesolution;cleaningsolution;andpHelectrodestoragebottle) -011007conductivitystandard,1413µS,5x60mL -Twoelectrodearmswithredesignedholders -Universalpoweradapter


AccessoriesCML # DescriptionVSTAR-PH Orion VERSA STAR pH/temperature moduleVSTAR-ISE Orion VERSA STAR pH/ISE/temperature moduleVSTAR-CND Orion VERSA STAR conductivity/temperature moduleVSTAR-RD OrionVERSASTARRDO/DO/temperaturemoduleVSTAR-LR OrionVERSASTARpH/temperaturewithLogRtechnologymoduleSTARA-BEA BenchtopelectrodearmforOrionStarA-seriesmetersSTARA-HB FreestandingbaseforusewithOrionStarA-seriesbenchtopelectrodearmSTARA-PWR Replacement universal power adapter for Orion VERSA STAR meters096019 OrionStarstirrerprobe8102BNUWP OrionROSSUltrapHelectrode,refillable,glassbody8172BNWP OrionROSSSure-FlowpHelectrode,glassbody8156BNUWP OrionROSSUltrarefillablepHelectrode,epoxybody8157BNUMD OrionROSSTriodepH/ATCelectrode,refillable,epoxybody8302BNUMD OrionROSSTriodepH/ATCelectrode,refillable,glassbody8172BNX OrionROSSSure-FlowpHelectrode,glassbody8611BNWP OrionROSSsodiumcombinationISE927007MD OrionATCprobe,stainlesssteelbody927005MD OrionATCprobe,epoxybody013005MD OrionDuraProbeconductivitycell,4-cell,K=0.475013016MD Orionconductivitycell,flowthrough,K=0.1083005MD Oriondissolvedoxygenprobe,polarographic086030MD Orionauto-stirDO/BODprobe,polarographic087010MD OrionRDOopticalDOprobe,3mcable810199 OrionROSSall-in-onepHbufferkit910104 OrionpH4.01buffer,475mL910107 OrionpH7.00buffer,475mL910110 OrionpH4.01buffer,475mL011008 Orionconductivitystandard,100µS/cm,5x60mLbottles011007 Orionconductivitystandard,1413µS/cm,5x60mLbottles011006 Orionconductivitystandard,12.9mS/cm,5x60mLbottles1010001 Orionconductivitycalibrationresistorkit9512HPBNWP Orionhigh-performanceammoniaISE9512BNWP Orion standard ammonia ISE951007 Ammoniastandard,1000ppm,475mL951210 Low-levelammoniaISA951211 AmmoniaISA,475mL951209 FillsolutionforOrionhigh-performanceammoniaISE,60mL951202 FillsolutionforstandardammoniaISE,60mL951213 AmmoniaISEstoragesolution9609BNWP OrionfluoridecombinationISE040906 Fluoridestandard,1ppmwithTISABII,475mL040907 Fluoridestandard,2ppmwithTISABII,475mL040908 Fluoridestandard,10ppmwithTISABII,475mL940909 TISABII,1gallon8611BNWP OrionROSSsodiumcombinationISE


Appendix Advanced Features

Automatic pH Buffer Recognition Feature

TheOrionVERSASTARMeterwithpH/ISE/TemperaturemodulesystemsarecapableofautomaticallyrecognizingpH1.68,4.01,7.00,10.01and12.46buffersorpH1.68,4.01,6.86,and9.18buffersduringapHcalibration,dependingonthepHbuffersetthatisselectedinthesetupmenu.Duringacalibration,themeterusestheselectedbuffersetandtherawmVreadingofthepHelectrodeinthebuffertorecognizeanddisplaythebuffervalueatthemeasuredtemperature.TherawmVreadingofthepHelectrodeinthebuffermustbeabout±30mVfromthetheoreticalmVreadingofthebufferinorderforthemetertoautomaticallyrecognizethebuffer.

USA pH Buffer Set DIN pH Buffer SetBuffer mV Range Buffer mV Range

1.68 +285to+345mV 1.68 +285to+345mV4.01 +207to+147mV 4.01 +207to+147mV7.00 -30to+30mV 6.86 +38to-22mV10.01 -207to-147mV 9.18 -99to-159mV12.46 -293to-353mV

Testing a pH Electrode for Automatic Buffer Recognition

ToverifythattherawmVreadingofthepHelectrodeinuseis±30mVfromthetheoreticalmVreadingofthebuffer,andthereforeverifythatthepHelectrodeinuseiscapableofperformingautomaticbufferrecognition,performthefollowingprocedure.

1. SetthemeasurementmodetomV.PreparethepHelectrodeaccordingtotheinstructionsinthepHelectrodeuserguide.

2. RinsethepHelectrodewithdistilledwater,blotitdrywithalint-freetissueandplacethepHelectrodeintoapH4.01bufferatapproximately25°C.

3. WaitforthemeasurementtostabilizeandrecordthemVvalueofthepH4.01bufferwhenthemeterindicatesthatthemeasurementisstable.ThereadtypeselectedinthesetupmenuwilldeterminehowthemVmeasurementsaredisplayedbythemeter.

4. RemovethepHelectrodefromthepH4.01buffer.

5. RinsethepHelectrodewithdistilledwater,blotitdrywithalint-freetissueandplacethepHelectrodeintoapH7.00bufferatapproximately25°C.

6. WaitforthemeasurementtostabilizeandrecordthemVvalueofthepH7.00bufferwhenthemeterindicatesthatthemeasurementisstable.

7. ThemVreadingofthepHelectrodeinpH4.01buffershouldbeintherangeof+207to+147mVandthemVreadingofthepHelectrodeinpH7.00buffershouldbeintherangeof-30to+30mV.IfthemVreadingsofthepHelectrodeareinthecorrectranges,thepHelectrodeiscapableofperformingautomaticbufferrecognition.IfthemVreadingsofthepHelectrodearenotinthecorrectranges,thevaluesofthepHbufferswillneedtobemanuallyenteredduringapHcalibration.


pH Electrode Condition Icon

TheelectrodeconditioniconindicatestheperformanceofthepHelectrode,basedonthelastsavedcalibration andelectrodemeasurementstability.

Icon pH Electrode Definition of Icon

Electrodeconditionisgoodandtheelectrodeslopeis90to110%.

Electrodeconditionisfairandtheelectrodeslopeis80to120%.

Electrodeconditionisbadandtheelectrodeslopeislessthan80%orgreater than120%.

Consulttheelectrodeuserguideforinstructionsonhowtoclean,conditionandtroubleshootthe electrode.


Multipoint Calibration Curve Feature

TheOrionVERSASTARMeterwithpH/ISE/Temperaturemodulesystemsutilizeapoint-to-pointschemeformultipointcalibrationsinthepHandISEmodes.Inthistechnique,straightlinesegmentsaredrawnbetweeneachsuccessivepairofcalibrationpoints.SeparateslopeandE0valuesarecalculatedusingtheadjacentpairofdatapoints.Forexample,inafivepointcalibration,fourslopesandfiveE0valuesarecalculatedtocorrespondtothefourlinesegmentsthatconnectthedata.pHorconcentrationvaluesforsamplesarecalculatedaccordingtothesegmentthattheirpotentialvaluesfallinto.Thoseaboveorbelowtherangeofcalibrationarecalculatedbyextrapolationfromthetoporbottommostsegment.

Thedisplayedslopeisanaverageslopeoftheseparatesegments.Theslopemaybeoutsidetherangenormallyexpectedforanelectrode.Forexample,iftheslopeofonesegmentislow,thentheaveragewillbelowerthantheoretical,eventhoughtheresponsemaybeNernstianovermostoftherange.

Thefigurebelowisanexampleofatypicalcalibrationcurveusingthismethod.


Automatic Blank (Non-linear) Correction Feature

TheOrionVERSASTARMeterwithpH/ISE/Temperaturemodulesystemshaveafeatureknownasautomaticblankcorrection.Inthistechnique,themeterdecideswhetherblankcorrectionisthebestmeasurementstrategybyanalyzingtheelectroderesponseduringamultipointcalibration.Aseparateblankdoesnothavetoberun.Graphically,automaticblankcorrectionisequivalenttodrawingasmoothcurvethroughthelowestthreepointsofthemultipointcalibrationandextrapolatingtozeroconcentrationbasedontheassumptionofNernstianelectrodebehavior.

Multipointcalibrationsatthelowerlimitofdetectionaredesirablewhentheresponseofanionselectiveelectrodeisnon-linearandcannotbecharacterizedwithaoneortwopointcalibration.Thisisusuallyseenasalowelectrodeslope.Generally,theelectrodeisbehavinginaNernstianmannerbuttheeffectofablankisbeingobserved.Seethefigurebelow.

Thisblankmaybeatruereagentblank,tracesofanalyteioninthereagentsoritmaybethe“mud”valueoftheelectrode.Itcouldalsobeaninterferenceinthereagentsthatbecomesapparentatlowlevelsofanalyteion,oritcouldbeanycombinationoftheseeffects.TheexpandedversionoftheNernstequationtraditionallyusedforblankcorrectionis as follows:

E = Eo + S * log (C + b) wherebistheblank

Inamulti-pointcalibration,asetofequationsisgeneratedandtherelationshipbetweenthemevaluated.Forexampletheequationsgeneratedinathreepointcalibrationwouldbeasfollows:

E1 = Eo + S * log (C1 + b)

E2 = Eo + S x log (C2 + b)

E3 = Eo + S x log (C3 + b)

ThemeterevaluatestherelationshipsbetweenthethreepotentialsE1,E2andE3andthethreeconcentrationsC1,C2 andC3.Iftherelationshipsindicatethatblankcorrectionisdesired,ablankwillautomaticallybecalculatedandthe non-linearitywillbecorrectedforinaNernstianmanner.Iftheappropriateconditionsarenotmet,theblankissetatzeroandeachsegmentofthemultipointcalibrationistreatedindependently.


Whenallthreeofthefollowingconditionsaremet,blankcorrectionisinvoked.

1. Theconcentrationofthefirststandardiszero,ortheslopeoftheelectrodebetweenthefirstandsecondstandardsislessthantheslopebetweenthesecondandthird.

2. Potentialdifferencesbetweenpointsaresignificant.Forexample,E3-E1>10mV

3. Theblankcorrectionalgorithmconvergesatreasonableblankandslopevalues.Conditionsinsteps1and2preventfailuretoconvergeinmostsituations.However,theslopewillattainanyvaluenecessaryforconvergencetoacalculatedblankvalueof3xC3.

Whenblankcorrectionisimplemented,theslopevaluefortheelectrodemaybeoutsidetherangeofvaluesnormallyconsideredacceptableduringanordinarycalibration.Forbestresults,calibrationstandardsshouldbecloseinrangetotheexpectedsampleconcentrationsandshouldbrackettheexpectedsampleconcentration.Whenconditions1,2and3arenotmet,thecalibrationdataishandledbythemultipointcalibrationmethoddescribedearlier.

Incalibrationswithmorethanthreepoints,acombinationofmethodsisutilized.Automaticblankcorrectionisusedifthelowestthreepointssatisfythecriteriaandmultipointcalibrationisusedfortheotherpoints.Theslopedisplayedaftercalibrationisanaverageoftheslopecalculatedintheautomaticblankcorrectionalgorithmandtheslope(s)foreachadditionalsegment.

Determining an Isopotential Value

1. Prepareseveralstandardswithconcentrationrangesoverthemeasuringrangeoftheelectrode.

2. Measurethemillivoltvalueofthestandardsatroomtemperature,about20°Cto25°C.

3. Measurethemillivoltvalueofeachstandardat75°C.

4. Measurethemillivoltvalueofeachstandardat10°C.

5. Onsemi-logarithmicgraphpaper,plottheconcentrationvaluesonthelogaxisversusthemillivoltvaluesonthelinearaxis.

6. Thelineswillintersectattheisopotentialvalue.Readtheconcentrationfromthegraphforthispointfromthelogaxis.Thisistheisopotentialvalue.


Notes

9 0 0 1 : 2 0 0 8

Water Analysis Instruments

North America 166 Cummings Center Beverly, MA 01915 USA Toll Free: 1-800-225-1480 Tel: 1-978-232-6000 [email protected]

NetherlandsTel: (31) 033-2463887 [email protected]

India Tel: (91) [email protected]

Japan Tel: (81) [email protected]

ChinaTel: (86) 21-68654588 [email protected]

Singapore Tel: (65) [email protected]

Australia

Tel: (613) 9757-4300in Australia (1300) [email protected]/water

© 2011 Thermo Fisher Scientific Inc. All rights reserved.

68X592004 Rev. A 12-11

thermo scientific orion versa star™ ph/ise/temperature ......thermo scientific orion versa star...

Documents