The VANASYL Bags

Vanasyl Vanasyl LLC.LLC.

Areas for Bag Technology Areas for Bag Technology ApplicationsApplications

Bioreactor samplingBioreactor sampling Bioreactor additions (antifoam etc.)Bioreactor additions (antifoam etc.) Media/buffer prep & storageMedia/buffer prep & storage Product storage & transferProduct storage & transfer Lab & pilot scale bioreactorsLab & pilot scale bioreactors Aseptic connections Aseptic connections

Vanasyl Bag Material: Multilayer Ethylene-Vinylacetate (EVA)

With A Barrier Film.(Film Gauge 400 μm @ 23°C)

Examples of Suitable Uses:

• Cell Culture• Amino Acids• Lipophylic Solutions• Product Storage and Transportation• Medium Storage and Delivery• Sampling Collection and more.

Issues with Accessing Issues with Accessing Samples in Bottles and Non-Vanasyl BagsSamples in Bottles and Non-Vanasyl Bags

Requires Safety CabinetRequires Safety Cabinet

Requires replacing a Bottle with a SeptumRequires replacing a Bottle with a Septum Risks contaminationRisks contamination Time ConsumingTime Consuming

Sample withdrawal requires syringe with needleSample withdrawal requires syringe with needle Needle-stick risk to personnelNeedle-stick risk to personnel Insurance factorInsurance factor

Time required compromises sample viabilityTime required compromises sample viability pH changepH change ContaminationContamination

Benefits of Vanasyl BagsBenefits of Vanasyl Bags

No needles- eliminating needle injuries and disposal issues.No needles- eliminating needle injuries and disposal issues.

No need for crimping- minimizing human error.No need for crimping- minimizing human error.

No need for a safety cabinet.No need for a safety cabinet.

No need to empty the bag.No need to empty the bag.

Faster sampling. A sample can be taken, anywhere from 10 to 30 seconds after Faster sampling. A sample can be taken, anywhere from 10 to 30 seconds after filling the sampling bag via a sterile syringe- ensuring sample integrity.filling the sampling bag via a sterile syringe- ensuring sample integrity.

Bag can be stored, then frozen down to 90°C, then brought back to room Bag can be stored, then frozen down to 90°C, then brought back to room temperature without glass transition and samples can continue being taken.temperature without glass transition and samples can continue being taken.

Pre-sterilized (gamma-irradiated).Pre-sterilized (gamma-irradiated).

No particles.No particles.

No leakage. No leakage.

Non-fragile.Non-fragile.

Technical Data Technical Data (more available upon request)(more available upon request)

Physical Properties Dimension Value Procedure

Water Vapor Transmission Rate

g/(m2.day.bar) 1.6 ASTM F-1249

Oxygen Permeability cc(m2.day.bar) .0000022 ASTM D-3985

CO2 Permeability cc(m2.day.bar) .000006 Mocon

Permatran C-IV

Cryogenic Suitability Celsius <-90

UV Transmission RateUV Transmission Rate

Available Bag SizesAvailable Bag Sizes

150cc, 250cc, 500cc, and 1,000cc. (As standard with the Clave® fitting; otherwise to the end user’s specifications)

2L to 25L and larger (The exact bag size, tubes, and fittings are to the end user’s specifications)

CONTACT INFOCONTACT INFO

Vanasyl LLC.

Director:

Tamara Fridman 347-675-5452