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The Second International Conference on

Basic and Applied Mycology

(ICBAM-2)

Assiut University, Assiut, Egypt

14 - 15 March 2015

Timetable and Abstracts

إتحاد الجامعات العربية

The Second International Conference on Basic and Applied Mycology, 14 – 15 March, 2015

1

The Second International Conference on

Basic and Applied Mycology (ICBAM-2)

Assiut University Mycological Centre (AUMC)

and

Society of Basic and Applied Mycology (SBAM)

In Collaboration with

the Association of Arab Universities

Emblem

Conidiophore of PENICILLIUM in a cartouche, that in ancient

Egyptian hieroglyphics, encloses names of royal or divine personages


14 - 15 March 2015


1

CONTENTS

- ICBAM-2 2015 Committees …………………………………………………… 2

- General Information .. ………………………………….……………,…………. 3

- Timetable……………………………………………………………………..…. 4

- Abstracts of Oral Presentations …………………………………………………. 9

- Abstracts of Poster Presentations ……………………………………………….. 37

- ICBAM-2 2015 Participants List ……………………………,,,………………… 43


2

ICBAM-2 2015 COMMITTEES

President: Professor Dr. Abdel-Aal H. Moubasher

Organizing Committee:

Prof. Abdel-Razik . A. Abdel-Razik Chairman Faculty of Agriculture

Prof. Mohamed B. Mazen Vice Chairman Faculty of Science

Prof. Ahmad M. Moharram Member Faculty of Science

Prof. Alaa El-Din A. Moubasher Member Faculty of Medicine

Dr. Soad Abdel-Latif Member Faculty of Pharmacy

Prof. Hussein Y. Ahmed Member Faculty of Vet. Medicine

Scientific Committee:

Prof. A. M. Moharram Chairman Faculty of Science

Prof. Sobhy I. Abdel-Hafez Vice Chairman Faculty of Science

Prof. Magdy M. K. Bagy Member Faculty of Science

Prof. Saad S.El-Maraghy Member Faculty of Science

Prof. Khairya M. Abdel-Gawad Member Faculty of Science

Prof. Mohamed A. El-Nagdy Member Faculty of Science

Prof. Abdel-Raof M. Khalil Member Faculty of Science

Prof. Mohamed A. Abdel-Sater Member Faculty of Science

Prof. Mady A. Ismail Member Faculty of Science

Prof. Abdel-Nasser A. Zohri Member Faculty of Science

Prof. Sayed. Ashour Ahmed Member Faculty of Agriculture

Prof. Mohamed Samy Mohamed Member Faculty of Agriculture

Prof. Farouk A. Abdel Galil Member Faculty of Agriculture

Prof. Fikry G. Fahmy Member Faculty of Agriculture

Prof. Ensaf Abdel-Majeed Member Faculty of Medicine

Prof. Asmaa A. Hussein Member Faculty of Veterinary Medicine


3

GENERAL INFORMATION

Conference Language

Oral papers, posters and abstracts will be in English

Conference Badges

Delegates are kindly requested to were their badges at all times.

Venue

The conference will be held at the Administration Building of Assiut University, Assiut, Egypt

P.O. Box: Assiut, Egypt 71526

Tel: +20882080153

Fax: +20882080153

E-mail: [email protected]

Web site: aun.edu.eg/aumc/aumc.htm

Accommodation and local transportation:

- Assiut University Guest Houses will be available for participants.

- Assiut University Hotel will be available at special rate.

- Organizing Committee will do the appropriate reservations for all participants.

- Assiut University will provide local transportation in Assiut for participants on arrival and departure.

mailto:[email protected]


4

TIMETABLE OF THE SECOND INTERNATIONAL CONFERENCE

ON BASIC AND APPLIED MYCOLOGY

Saturday, 14 March, 2015

8.30 – 10.00

Registration, Loading Presentations, Posters up

(Octagonal Hall, Administration Building of Assiut University)

10.00 – 10.30

Opening Ceremony

10.30-11.00

[O-1]

Opening Lecture: Abdel-Aal Hassan Moubasher

Department of Botany and Microbiology, Faculty of Science & Director of the Assiut

University Mycological Centre, Assiut University, Egypt.

Email: [email protected]

Challenges confronting Mycology, with a synopsis on changes in the Code of

Nomenclature of Fungi

11:00 -11:30

Coffee & Tea Break

11:30 – 13:00

SESSION 1: ENVIRONMENTAL MYCOLOGY

Chairpersons: Abdel-Razik Abdel-Aleem & Saad Shehata El-Maraghy

11.30-12.00

[O-2]

Abdel-Wahid Fahim Moustafa

Department of Botany, Faculty of Science, Suez Canal University, Ismailia, Egypt.


On the biology of fungi in habitats of high water stress

12.00- 12:20

[O-3]

Saeed Monassar Saeed Alghalibi, Qais Yusuf Abdullah & Aida Q. M. Al-Zeqry

Department of Biology, Faculty of Science, Sana’a University, Yemen.


Mycobiota associated with Yemeni mummies and their extracellular enzyme

abilities

12:20-12:40

[O-4]

Mohamed Hani Abdel-Aal H. Moubasher

Department of Botany and Microbiology, Faculty of Science, Cairo University, Egypt.


Phylogenetic analysis of some members of Sections Nidulantes and Versicolores of

Aspergillus using DNA sequences

12:40- 13:00

[O-5]

Abdel-Aal Hassan Moubasher1, Mady Ahmed Ismail

1, Nemmat Abdel Gawad

Hussein1 & Hassan Abdel-Motagally Gouda

2

1Department of Botany & Microbiology, Faculty of Science, Assiut University, Assiut,

Egypt, 2Agricultural Research Center, Assiut, Egypt.


Osmophilic, osmotolerant, halophilic and halotolerant mycobiota of soil of Wadi

El- Natrun region, Egypt

13:00-15:00

Lunch Break






5

15:00 – 16:00

SESSION 2: FUNGI AS BIOCIDES

Chairpersons: Abdel-Wahid F. Moustafa & Mohamed Saeed El-Zemaity

15.00-15:20

[O-6]

Ahmed Yousry Ellaithy

National Research Center, Egypt.


Biological control of the two spotted spider mite Tetranychus urticae with

relevance to pathogenic fungi and host plant reaction, case study of plastic house

roses

15.20-15.40

[O-7]

Saad Shehata El-Maraghy1, Mohamed Ahmed Abdel-Sater

1, Mohamed A. Abdel-

Rahman2, Khalid A. Hussein

1 & Shaimaa Hassan

1

1 Botany and Microbiology Department, Faculty of Science, Assiut University,

2Plant

Protection Research Institute, Agricultural Research Center, Assiut.


First record of the entomopathogcnic fungus Beauveria bassiana (Balsamo) Viullemin

as endophyte in Assiut

15.40-16:00

[O-8]

Saad Shehata El-Maraghy1, Mohamed A. Abdel-Rahman

2, Azza M. A. Awad

3,

Youssef M. Omar4 & Asmaa H. M. Mohamed

2

1Department

Botany and Microbiology, Faculty of Science, Assiut University,

Egypt;2Plant Protection Research Institute,

2Agricultural Research Center, Assiut,

Egypt; 3Zoology Department, Faculty of Science, Assiut University, Egypt.

4Plant Protection Department, Faculty of Agriculture, Assiut University, Egypt.


Incidence of mycopathogens of the oat bird-cherry aphid, Rhopalosiphum padi L.

(Homoptera: Aphididae) infesting wheat plants in Assiut.

16:00-16:30

Coffee & Tea Break

16:30 – 18:10

SESSION 3: FUNGI AS BIOCIDES

Chairpersons: Sayed Ashour Ahmed, Farouk A. Abdel-Galil & Abdellah Abdel-

Moniem Saad

16:30-16:50

[O-9]

Mohamed Saeed El-Zemaity

Department of Plant Pathology, Faculty of Agriculture, Ain Shams University, Egypt. E- mail: [email protected]

Developing of fungal biopesticide formulations

16:50-17:10

[O-10]

Bassam Fikry G. Fahmy1, Nesreen Mohamed Fahmy

2, Samir H. Manaah

2 &

Mohamed Fahmy Abo-Gadeer2

1Plant Protection Research Institute, Agricultural Research Center,

2Plant Protection, Faculty of Agriculture, Assiut University, Egypt.


Occurrence of entomopathogenic fungi in grain aphids in Upper Egypt, with

reference to certain pathogenic tests

17:10-17:30

[O-11]

Ramadan Abdel-Ghani Mohamed, Osama Abdel-Hafeez Al-Bedak, HodaA. M.

Ahmed, Nemmat Abdel-Gawad Hussein and Heba Elsalahy

Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut,

Egypt.


Reliable analytic strategy to correlate the morphological and cytological

parameters on Lupinus termis L. against Fusarium oxysporum infection






6

17:30 – 17:50

[O-12]

Rabab Majead Abed1, Hadi Mahdi Aboud

2 & Ali hashim Al-Mousawi

3

1 Department of Biology -College of Education for Pure Science, University of Diyala,

Diyala, Iraq, 2Directorate of Agriculture Research-Ministry of Science and

Technology. Baghdad - Iraq, 3

Department of Biology, College of Science for Women-

University of Baghdad. Baghdad - Iraq.


Ability of some bioagent fungi to promote resistance of cucumber (Cucumis

sativus L.) against the pathogenic fungus Rhizoctonia solani

17:50 – 18:10

Posters Session

Sunday 15 March, 2015

9:00 – 11:00

SESSION 4: NEW APPROACHES OF FUNGI & MYCOTOXINS

Chairpersons: Mohamed Saad Abou El-Soud & Ahmed Y. Nassar

9:00-9:30

[O-13]

Mohamed Ali Ahmed

Department of Plant Pathology, Faculty of Agriculture, Ain Shams University, Cairo,

Egypt.


New approaches and applications of some fungi

9:30-10:00

[O-14]

Metwally Ramadan Kottb



Role of Trichoderma volatiles in soil

10:00-10:20

[O-15]

Ismail Seddiq Mohamed1, Mohamed Wail Abd-Alazeem

2, Serageldin Sultan

2 &

Hams Mohamed Ahmed2

1Department of Microbiology and Immunology, faculty of Medicine, Assuit University

2Department of Microbiology, faculty of Veterinary Medicine, South Valley University,

Egypt.

E-mail : [email protected]

Isolation and identification of fungi and their mycotoxins from milk

10:20-10:40

[O-16]

Amal Mokhtar1 & Ahmed M. Ali

2

1Department of Microbiology and Immunology, Faculty of Veterinary Medicine, Aswan

University, 2Chemistry Department, Faculty of Science, Al-Azhar University, Assiut,

Egypt.


Threating mycotoxins in the urine of ewes in Upper Egypt

10:40-11:00

[O-17]

Hala Jameel Al-Jebouri, Ahmed Lotfy E. Mahmoud & Abdullah Yahya Al-Mahdi

Biology Department, Faculty of Science, Sana’a University, Yemen.


Natural occurrence of Fusarium mycotoxins (fumonisins, zearalenone and T-2

Toxin) in corn for human consumption, Yemen

11.00 – 11.30 Coffee & Tea Break





7

11:30 -13:40 SESSION 5: MEDICAL MYCOLOGY

Chairpersons: Alaa-Eldin A. Moubasher, Nagwa Abdel-Azeem & Mohamed A.

Abdel-Sater

11:30 -12:00

[O-18]

Syed Mohamed Hasnain1 & Abdulrahman S. Alfrayh

2

1Department of Cell Biology, King Faisal Specialist Hospital and Research Centre,

Riyadh, Saudi Arabia, 2Department of Pediatrics, College of Medicine, King Saud

University, Riyadh, Saudi Arabia.


Role of indoor and outdoor airborne fungi in the development of respiratory

allergic diseases in Saudi Arabia

12:00 -12:20

[O-19]

Sahar Abdelomez Ismail

Department of Dermatology, Venereology and Andrology, Assiut University, Egypt.


Understanding dandruff: role of fungi

12:20 -12:40

[O-20]

Hisham Diab1 & Ahmad Mohammad Moharram

2

1Department of Dermatology and Venereology, Faculty of Medicine, Assiut University,

2Department of Botany and Microbiology, Faculty of Science, Assiut University, Egypt.

E-mail:

Presence of fungi in scalp of patients with alopecia areata: triggering factor or

coexistence

12:40 -13:00

[O-21]

Ali Mohamed Hassan Sallam

Department of Microbiology, Faculty of Science, Taiz University, Taiz, Yemen.


Mycological studies on dermatomycotic diseases in Taiz City, Yemen

13:00 -13:20

[O-22]

Nadir Musa Khalid Abuzeid1, Mohamed Bashir

1, Eltaib Abdallah

1, Abdallah

Bashir1, Abdalmajed Mohammed

1, Mohammed Yousif

1, Elmahdi Mohammed

1,

Abdallah Adam2, Awatif Eltayeb

3, Elham Abelbasit

4 & Yousif Tybin

5

1Omdurman Islamic University, Faculty of Medical laboratory Science, Department of

Clinical Microbiology, Omdurman Sudan.2Omdurman Islamic University, Faculty of

Sciences and Biotechnology, Department of Microbiology, Omdurman

Sudan.3Khartoum University, Faculty of Sciences, Department of Botany, Khartoum-

Sudan.4National Research Centre, Veterinary Medicine, Department of Mycology,

Khartoum-Sudan.5Bahary University, Faculty of Medicine, Department of

Dermatology, Bahary-Sudan.


Ecological studies on Cryptococcus species inhabitant in the Sudan

13:20 -13:40

[O-23]

Ahmad Mohammad Moharram¹, Ismail Seddik Mohamed², Ibrahim² M. A. &

Fatma Sayed Abdel-Sameii²

¹Department of Botany and Microbiology, Faculty of Science, ²Department of

Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut.


Mycotic infections in diabetic foot patients

13:40-15:00

Lunch Break

15:00- 17:00

SESSION 6: FUNGAL BIOTECHNOLOGY

Chairpersons: Ahmed Zaki & Reda El-Tohamy




8

15:00-15:20

[O-24]

Abdel-Nasser Ahmed Zohri

Department of Botany and Microbiology, Faculty of Science, Assiut University, Egypt.


Some important therapeutic compounds produced by fungi

15:20-15:40

[O-25]

Ahmad Mohammad Moharram



Macrofungi as a precious source of pharmaceutical compounds

15:40 -16:00

[O-26]

Ahmed Abdel-Fattah Shoreit, Mady Ahmed Ismail, Ahmad Mohammad

Moharram & Mohamed H. A. Hassan



Biogenic silver nanoparticles of resistant Aspergillus flavus AUMC 9834 against

some pathogenic microorganisms and their synergistic effect with antibiotic

fluconazole

16:00 – 16:20

[O-27]

Naeima Mohamed Hammam Yousef



Role of biotechnology in production of HBV vaccine in Saccharomyces cerevisiae

16.20 – 17:00

Coffee & Tea break

SESSION 7 : CLOSING SESSION

Chairpersons: Abdel-Aal H. Moubasher, Abdel-Razik Abdel-Aleem & Ahmad M. Moharram

17:00 -18:00

Closing Remarks, Best Poster Award, Posters Down



9

Abstracts of

ORAL PRESENTATIONS


10

O1 CHALLENGES CONFRONTING MYCOLOGY, WITH A SYNOPSIS OF CHANGES IN THE

CODE OF NOMENCLATURE OF FUNGI

Abdel-Aal Hassan Moubasher

Department of Botany and Microbiology, Faculty of Science & Director of the Assiut University Mycological

Centre, Assiut University, Egypt.


Mycology is critically challenged at present and in the future by the following factors:

1- Globalization and its influence on the rapid flow of information through the internet and other means of

modern communications, that put a heavy burden on mycologists to keep aware of the enhanced

advancement of knowledge.

2- Global warming due to increase in environmental disruption, primarily because of human activities, and its

adverse consequences on living organisms including fungi.

3- Rapid development in molecular biology techniques which stimulates fast flow of initiation of new

species, genera and even higher taxa of fungi.

4- Growing implementation of fungi in biotechnology for the production of indispensable substances, and the

mycologists are urged to search for more valuable products in known and new fungi.

5- Fungi are attracting more attention among the medical community, as the number of fungi involved in

human diseases has increased enormously in the last few decades, especially in immunity- deficient

patients.

6- Mycology, like other taxonomy sciences of life, is not inspiring for the junior generation of biologists, who

are reluctant to establish their future career in it. They are fascinated by other, especially applied,

specializations that may also secure higher- salary jobs.

7- Only about 7 % of the total number of species of fungi, suggested to be 1.5 million species, are known,

and the remaining huge numbers are waiting for discovery.

8- There have been tremendous changes in the names of genera and species epithets to comply with

taxonomic rules.

9- Fungi are continuously isolated from the natural substrata and cultivated on isolation and diagnostic media

for identification, all of them are selective.

10- Cultures of fungi have to be preserved alive for academic and biotechnological purposes.

11- Dual names of Ascomycota and Basidiomycota which have anamorph (asexual) phase and teleomorph

(sexual) phase with the primacy of the teleomorphic name, have been confusing for the users e.g. plant

pathologists, human pathologists, fungal biotechnologists, and fungal geneticists who are not interested in

taxonomic affairs.

Dramatic changes in the International Code of Nomenclature of Fungi have been decided in the

International Congress of Botany held in Melbourne in 2011. The most significant of them is the

abandonment of the dual names system to one fungus, one name system, which means that the two names

compete equally for priority; priority is given to the oldest genus or species name, irrespective of whether

they were originally described for teleomorphs or anamorphs. A synopsis of these changes is given.


11

O2 ON THE BIOLOGY OF FUNGI IN HABITATS OF HIGH WATER STRESS

Abdel-Wahid F. Moustafa



In biology, using the term water content very often could be misleading because not all water in the environment

(or substrate) is effective i.e. available, while part is firmly adhered to the organic particles or inorganic ions and

accordingly unavailable, the other part is unbound i.e. available or utilizable and thus usually referred to as “the

effective water content”. The term water activity (aw) has been adopted as a measure unit to express the

amount of unbound available water. aw is associated with relative humidity in the following mathematical

relation:

aw = RH/100 and RH = P/P0 X100 where:

P: vapour pressure of the solution and P0: vapour pressure of pure water.

Organisms (including fungi) able to grow at low values of aw due to shortage of water are classified as xerophilic

or xerotolerants while these organisms able to grow at low values of aw due to the presence of high salt

concentrations are usually regarded as halophilic or halotolerants. Members of both groups (xero- or halo) are

well adapted to cope with the life in such stressful habitats, suffering from either water shortage or high salinity,

by accumulating or developing compatible solutes inside their protoplasm as osmoregulators to correct the

osmotic tension on either side of the cell membrane.


12

O3 MYCOBIOTA ASSOCIATED WITH YEMENI MUMMIES AND THEIR EXTRACELLULAR

ENZYME ABILITIES

Saeed M. S. Alghalibi, Qais Y. M. Abdullah and Aida Q. M. Al-Zeqry

Department of Biology, Faculty of Science, University of Sana'a, Republic of Yemen.


Biodeterioration is considered a great factor in the decomposition of the Yemeni mummies. In this study, 5

Yemeni mummies' samples (linen bandages and some deteriorated mummies) collected from National museum

were investigated. Fourteen fungal species belonging to 8 genera were isolated and identified. The most common

species were Aspergillus niger (23.40% of the samples), A. flavus, Cladosporium cladosporioides (10.63% each),

P. echinulatum, Penicillium sp. (6.38% each), A. candidus, A. fumigatus, A. ustus and Cladosporium herbarum

(4.25% each), Aureobasidium pullulans, Scopulariopsis koningii, Chaetomium thermophilum, Stachybotrys

chartarum, Mucor circinelloides, Ulocladium chatarum and Trichoderma hamatum (2.12 % each). Twenty three

isolates were examined for their ability to produce cellulase, chitinase and pectinase. Among these, fourteen

isolates exhibited enzymatic potential. These enzymes can play an important role in deteriorating linen bandages

as well as the mummy bodies. The cellulase was produced by 8 isolates attributed to A. ustus, A. pullulans, S.

chartarum, C. thermophilum, U. chatarum, C. cladosporioides, A. candidus and Pencillium sp. Chitinase was

produced by A. niger and Pencillium sp., while pectinase was produced by Penicillium echinulatum, Trichoderma

hamatum, Scopulariopsis koningii and Mucor circinelloides.



13

O4 PHYLOGENETIC ANALYSIS OF SOME MEMEBERS OF SECTIONS NIDULANTES AND

VERSICOLORES OF ASPERGILLUS USING DNA SEQUENCES

Moubasher, Hani1; Ali, Mai

2; Abo El-Kassem, Nabil

2; Ali, Refat

2.

1Botany & Microbiology Department, Faculty of Science, Cairo University, Giza ;

2Botany Department, Faculty

of Science, Fayoum University, Fayoum, Egypt.

E-mail:[email protected]

Eight fungal isolates, which belong to 7 species of Aspergillus, were used in this investigation, RAPD analysis

using six arbitrary primers recorded variations between, four isolates. The Aspergillus isolates were classified into

two main clusters (cluster I and cluster II). Cluster II included Aspergillus aegyptiacus isolate (AUMC 3603)

only, while cluster I was splitted into two subclusters (A and B). Subcluster A included: E. nidulans; E. rugulosa

and subcluster B, included only E. variecolor. Sequencing of ITS gene showed only one unique band ranging

from 250- 270 bp in ITS1 region and 320-350 bp in ITS2 region. Phylogenetic tree was displayed using the

TREEVIEW program (Mega4 and ClastalW2). ITS1 of the eight Aspergillus isolates could be divided into two

clusters. The first cluster consisted of E. nidulans, E. variecolor and E. rugulosa at value 1.2 and subcluster

consisted of E. nidulans and E. variecolor at value 0.5. The second cluster included the two isolates of

Aspergillus aegyptiacus (AUMC 3603&6122), A. sydowii and A. versicolor at value of 0.5 and A. caespitosus at

value 2.4 and subcluster included the two isolates of Aspergillus aegyptiacus, A. sydowii and A. versicolor at

value 1.7. Depicting the genetic relationship among Aspergillus spp. revealed that E. nidulansis closely related to

E. variecolor and the two isolates of Aspergillus aegyptiacus are closely related to A. sydowii, A. versicolor in

phylogeny. Based on the length of ITS2 sequences, the five Aspergillus isolates tested .could be divided into two

clusters. The first cluster consisted of Emericella species and one isolate of Aspergillus aegyptiacus (AUMC

3603) at value 0.58; the subcluster at value 0.25 included E. nidulans, E. variecolor and E. rugulosa. The second

cluster included only one isolate of Aspergillus aegyptiacus (AUMC 6122) at value 0.75. Depicting the genetic

relationship among Emericella species revealed that E. variecolor and E. rugulosa are closely related at value 0.1

in phylogeny. The above results lend further support to the recommendation that A. aegyptiacus is closely related

to section Versicolores, rather than section Nidulantes.



14

O5 OSMOPHILIC, OSMOTOLERANT, HALOPHILIC AND HALOTOLERANT MYCOBIOTA OF

SOIL OF WADI EL-NATRUN REGION, EGYPT

Abdel-Aal H. Moubasher, Mady A. Ismail, Nemmat A. Hussei and Hassan A. Gouda

Botany & Microbiology Department, Faculty of Science, Assiut University, Assiut, Egypt.


Ninety- six samples of newly reclaimed soil were collected from around the 8 main lakes of Wadi El-Natrun

region. Chemical analysis of these samples revealed that they are alkaline with pH 8.2–9.05, organic matter

content from 0.3 to 2.0% and total soluble salts from 1.7-30.5. Soil collected from Al Gaar Lake showed the

highest levels of moisture content, total soluble salts, potassium, carbonate, bicarbonate, sodium, calcium,

magnesium and chlorides. On the other hand, some parameters showed their peaks in other lakes, namely sodium

(11.1 mg/g) in Umm Risha, calcium (0.2 mg/g) in Hamra and magnesium (0.04mg/g) in AL-Beida. A total of 33

genera, 104 species and 3 varieties were recorded from the tested soil samples. The widest spectrum of species

(83 species) was isolated on the control medium whereas the lowest (45) appeared on 10% NaCl medium.

Aspergillus (15 species), Fusarium (12), Myrothecium (3) Stachybotrys (2), Penicillium (7) and Emericella (5)

were regularly the most dominant genera possessing the highest proportions of propagules on the three isolation

media. On the other hand, A. niveus, Chaetomium globosum, C. olivaceum, C. monocereus, F. poae,

Scopulariopsis (4 species), Scytalidium lignicola and Thermoascus aurantiacus were only isolated on 10% NaCl

medium. Aspergillus showed its count peak in Khadra Lake on the control medium, in Fasida Lake on 40%

sucrose and 10% NaCl medium. From Aspergillus, A. flavus, A. fumigatus, A. niger, A. ochraceus, A. sydowii and

A. terreus, in addition to P. chrysogenum and F. solani were common on all isolation media. Emericella and

Eurotium were the most common genera on 40% sucrose Czapek`s medium.



15

O6 BIOLOGICAL CONTROL OF THE TWO SPOTTED SPIDER MITE TETRANYCHUS URTICAE

WITH RELEVANCE TO PATHOGENIC FUNGI AND HOST PLANT REACTION, CASE STUDY OF

PLASTIC HOUSE ROSES

Ahmed Y. M. Ellaithy

National Research Center, Egypt.


The present study concerns problems encountered with practices of the two spotted spider mite Tetranychus

urticae biocontrol in roses open field and plastic houses which coincided with mildew diseases caused by

Sphaerotheca pannosa and Peronospora sparsa. Former studies revealed a positive relationship between

powdery mildew causative organisms and phytoseiid predatory mite in form of being an alternative food or

increasing prey consumption. Also the mutual relationship between mildew fungi and spider mite infestations are

also debatable between positive or negative influence. However lacking investigations with downy mildews

enhanced the present work. Field observation of T. urticae Biocontrol in roses revealed a stable interaction

between prey and predatory mite during summer and early fall when downy mildew started to flourish on roses

leaves which followed by exterminate of predatory mite Amblyseius californicus McGregor due to change of

plant odors or signals associated with P. sparsa fungi. Such observations induced investigating the interactive

relationship between host plant roses and pathogenic fungi of mildew diseases Sphaerotheca pannosa and

Peronospora sparsa, which could be named interactive defense mechanism. From one side and secondly the

interaction to call natural enemies for either of spider mite or fungi, plant hormones secreted after infestations and

synthesized secondary metabolites and their influence on predatory mites fitness and behavior. Therefore,

integrated control in plastic house roses may be reshaped.


16

O7 FIRST RECORD OF THE ENTOMOPATHOGCNIC FUNGUS BEAUVERIA BASSIANA

(BALSAMO) VIULLEMIN AS ENDOPHYTE AT ASSUIT, EGYPT

Saad S. Mohamed El-Maraghy1, Mohammed Abd-El Sater

1, Mohamed A. A. Abdel-Rahman

2, Khalid A.

Hussein1 & Shimaa Hassan

1

1Botany and Microbiology Department, Faculty of Science, Assiut University;

2Plant Protection Research Institute,

Agricultural Research Center, Egypt.


A novel biotechnology of using the beneficial microorganisms to improve plant health and productivity has been

extensively concerned as an endophyte, entomopathogenic fungi may play a role in protecting plants from

herbivory and disease. Beaveria bassiana is a fungal entomopathogen with the ability to colonize plants

endophytically. In the current study, 30 different samples representing 5 plant species were surveyed for

entomopathogenic fungi present as endophyte in leaves, shoots, and roots of wheat, corn, caraway, cumin, tomato

and coriander. B. bassiana was successfully isolated from the shoot of tomato (Solanium lycopersicum).

Identification of the isolated B. bassiana was confirmed by scanning electron microscopy technique. The going

study project now is to apply the same fungal isolate to other different plants and re-isolate this endophytic B.

bassiana strain. Moreover, the pathogenicity of the same strain will be tested against some economic insect pests

e.g. Spodoptera littoralis (cotton leaf worm) at different insect stages.


17

O8 INCIDENCE OF MYCOPATHOGENS OF THE OAT BIRD-CHERRY APHID,

RHOPALOSIPHUM PADI L. (HOMOPTERA: APHIDIDAE) INFESTING WHEAT PLANTS IN ASSIUT

Saad S. Mohamed El-Maraghy1, Mohamed A. A. Abdel-Rahman

2, Azza M. A. Awad

3 and Youssef M.

Omar4, Asmaa H. M. Mohamed

2

1Botany and Microbiology Department, Faculty of Science, Assiut University, Egypt;

2Plant Protection Research

Institute, Agriculture Research Center, Egypt; 3Zoology Department, Faculty of Science, Assiut University,

Egypt; 4Plant Protection Department, Faculty of Agriculture, Assiut University, Egypt.


The present investigation was conducted in Assiut (Abnoub district) during the wheat growing seasons of 2013

and 2014. The objective was to study the incidence of mycopathogens of oat bird-cherry aphid, Rhopalosiphum

padi L. infesting wheat plants naturally under field conditions. Six species of entomopathogenic fungi belonging

to Entomophthorales and Hyphomycetes were identified. Entomophthorales was represented by four species

belonging to the following three families; Ancylistaceae (Conidiobolus sp.), Entomophthoraceae (Entomophthora

planchoniana Cornu and Pandora (= Erynia) neoaphidis (Remaudierel & Hennebert) Humber) and family

Neozygitaceae (Neozygites fresenii). The hyphomycetous fungi were represented by two species Beauveria

bassiana (Balsmo.) Vuill. and Verticilium lecanii. Data showed that the aphid began to infest wheat plants early

during the middle of January when wheat plants were in the stem-elongation stage. Thereafter the number of

aphids increased gradually to reach a peak of abundance, when the plants were at the flowering stage during the

third week of February. During the next three weeks the number of the oat aphid declined sharply. The rate of

insect mortality with became greater by the end of January till the end of March. The number of cadavers

increased gradually to reach the maximum level by the end of March.



18

O9 DEVELOPING OF FUNGAL BIOPESTICIDE FORMULATIONS

El-Zemaity M. E.

Faculty of Agriculture, Ain Shams University, Egypt.

E- mail: [email protected]

وإبتكار المبيدات الحيوية وتوظيفها ضمن مكونات برامج اإلدارة المتكاملة لآلفات، وتعتبر المستحضرات المجهزة من تتزايد الحاجة لتطوير

رية الميكروبات أو منتجاتها بما في ذلك الفطريات أحد أهم خيارات تكتيكات إدارة اآلفة في العديد من األحوال. وقد فتحت المستحضرات التجا

فطريات وتزايد الطلب عليها الباب واسعا أمام الباحثين ورجال الصناعة من أجل إكتشاف وتطوير مواد جديدة فعالة تجاه مدى الناجحة المجهزة من ال

تعريفها أوسع من اآلفات، وفي الحقيقة فإن هناك العديد من البحوث التى إهتمت بدراسة النشاط الحيوى لفطريات معزولة محليا تجاه بعض اآلفات، و

ئصها المميزة، وتحديد الظروف المثلى لتنميتها، وغيرها من النقاط البحثية. ولكن فإن الدراسات المتعلقة باإلنتاج على نطاق واسع وتوسيم خصا

جهزة من والتجهيز بالمواصفات القياسية لمالئمة التطبيق الحقلى تعتبر قليلة ومحدودة جدا. والشك أن تحقيق نقلة نوعية لتوفير منتجات تجارية م

فطريات التى أثبتت نجاحا على المستوى المعملى، يتطلب إغتنام الفرص المتاحة وتضافر جهود العلميين المتخصصين في وقاية النبات ورجال ال

أو الصناعة معا إلنتاج مواد بمواصفات قياسية محددة. وتشجيعا للجهود الرامية لتطوير وإبتكار المبيدات الحيوية سواء على المستوى البحثى

صناعى ونشر إستخدامها على أوسع نطاق فإن هذه الورقة تركز على الخطوات القياسية إلنتاج وتطوير المستحضرات المجهزة من الفطريات ال

.وأيضا مناقشة المحددات والتحديات التطبيقية لهذه المواد المبيدة لآلفات الرئيسية خاصة الحشرات والفطريات المسببة لألمراض النباتية والحشائش،



19

O10 OCCURRENCE OF ENTOMOPATHOGENIC FUNGI IN GRAIN APHIDS IN UPPER EGYPT,

WITH REFERENCE TO CERTAIN PATHOGENIC TESTS

Bassam F. G. Fahmy1, Nesreen Mohamed Fahmy Abou-Gadir

2, Samir H. Manaah

2 and Mohamed F. Abo-

Gadeer2

1Plant Protection Research Institute, Agricultural Research Center,

2Plant Protection, Faculty of Agriculture,

Assiut University, Egypt.


The study was carried out in cultivated wheat fields at three governorates of Upper Egypt (Assiut, Sohag and

Qena). The entomopathogenic fungi naturally infecting cereal aphids were surveyed, and identified. Four

entomopathogenic fungi: Cladosporium cladosporioides (Fresen.) de Vries, Beauveria bassiana (Balsamo)

Vuillemin , Paecilomyces variotii Bainier, and Metarhizium anisopliae (Metchnikoff) Sorokin were isolated

from the grain aphids Sitobion avenae (Fabricius). The laboratory pathogenicity tests were carried out to explain

the sensitivity and ability of the subjected aphids for fungal infestation. The mortality of grain aphids due to these

fungi was assayed and evaluated. C. cladosporioides showed great ability to spread its hyphae and condiospores

on the target aphid and killed it. Moreover, the condiospores germinated forming germ tube and infective spear-

like hyphae for mechanical pressure and to facilitate the direct penetration of insect cuticle. Scanning electron

microscopy of B. bassiana showed condiogenous cells carrying spherical or subspherical conidiospores which

penetrate the integument of insects. The entomopathogenic fungus P. variotii was also able to infect and kill the

target insects. Data also revealed that the close attachment of the fungal mycelium to the grain aphid cuticle may

accelerate its germination and conditioning it for penetrating the target host .Data of scanning electron

microscope is considered the perfect tool to investigate the mode of infection and colonization of host insects by

the entomopathogenic fungi.


20

O11 RELIABLE ANALYTIC STRATEGY TO CORRELATE THE MORPHOLOGICAL AND

CYTOLOGICAL PARAMETERS ON LUPINUS TERMIS L. AGAINST FUSARIUM OXYSPORUM

INFECTION

Ramadan A. Mohamed1; Osama A. Al-Bedak

2; Hoda A.M.Ahmed

3; Nemmat A. Hussein

1 and H. Elsalahy

1

1Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut, Egypt;

2Assiut University

Mycological Centre, Faculty of Science, Assiut University, Assiut, Egypt; 3Plant Pathology Res. Inst., Agriculture

Research Centre, Giza, Egypt.


There are many studies that rely on a variety of different measurements to find a relationship between the parasite

and the reaction of the host in the wilt disease, but the question remains, which of these measurements has strong

or weak correlation with the Pathological case. Additionally, the correlation between these measurements could

give an explicit image or a real strength of the fungal isolation and its impact on the plant. This study was

conducted on 30 isolates of F. oxysporum isolated from Lupinus termis L. Root rot was ranged 60 to 80%, and

wilt was from 73.33 to 93.33% of the total plants. Some of cellular, morphological and physical measurements

were conducted on 8 isolates (out of 30 of the F. oxysporum). All of the collected data that describes the plant

response against the pathogenic strains were analyzed using Canoco package software to determine the fungal

groups which cause wilt disease. Hierarchical clustering analysis resulted in dividing the isolates into two groups;

A and B caused wilting of lupine in different severities. Result of DCA separated the epidermal cell parameters in

independent group and the rest of the measured parameters in another group. The CCA results showed that most

of the measured parameters on leaf No. 4, except leaf cell number, expressed highly about the wilting disease.

That makes these parameters are valuable and sensitive for any changes to wilting strength. This study aims to

use a reliable analytic strategy to differentiate between isolates using the appropriate scale to diagnosis wilting

disease accurately. More importantly, the ordination method of the used package cleared that AUMC9272 was

the highly severe isolate that cause wilt disease



21

O12 ABILITY OF SOME BIOAGENT FUNGI TO PROMOTE RESISTANCE OF CUCUMBER

(CUCUMIS SATIVUS L.) AGAINST PATHOGENIC FUNGUS RHIZOCTONIA SOLANI

Rabab Majead Abed1, Hadi Mahdi Aboud

2 and Ali Hashim Al-Mousawi

3

1Department of Biology, College of Education for Pure Science, University of Diyala, Diyala, Iraq,

2Directorate

of Agriculture Research, Ministry of Science and Technology. Baghdad, Iraq, 3Department of Biology, College of

Science for Women, University of Baghdad. Baghdad, Iraq.


The bioagent fungi Trichoderma harzianum and Glomus mosseae showed the ability to strengthen several defense

in cucumber plant against Rhizoctonia solani. This study included measurement of the activity of salicylic acid

(SA) hormone, the amount of total phenolic compounds and the amount of lignin. The ability to increase these

defensive parameters was shown in plants treated with T. harzianum or G. mosseae or there interaction in

presence and absence of R. solani. The treatment with T. harzianum + G. mosseae + R. solani showed highest

level in SA activity up to 0.7281 mg, and the treatment with T. harzianum + R. solani resulted in significant

increase in the amount of total phenol and lignin up to 1.898 and 18.099 mg respectively. These defensive

activities positively affected the growth parameters of cucumber plant whether the pathogen fungus was present

or absent. These parameters included shoot height, fresh and dry weight for shoot and root parts and root length.


22

O13 NEW APROACHES AND APPLICATIONS OF SOME FUNGI

Mohamed Ali Ahmed

Department of Plant Pathology, Faculty of Agriculture, Ain Shams University, Cairo, Egypt.


مناهج وتطبيقات حديثة للفطريات

الفطريات فيما بينها كما تتواصل مع ما حولها من كائنات أخرى , فعلى سبيل المثال تلعب فطريات الميكوريزا Communicateتتواصل mycorrhizae ة مع جذور النباتات , فانها تكون دورا كبيرا فى تواصل النباتات عبر جذورها , ففى الوقت الذى تتبادل فيه هذه الفطريات المنفع

حية شبكة واسعة من الهيفات الفطرية تحت األرضية تربط النباتات بواسطة جذورها مكونة نظام اتصاالت معقد , محذرة النباتات من الكائنات الاألخرى المتصلة بها عبر شبكة هيفات األخرى الضارة , فالنباتات التي تنتشر عليها حشرات المن قادرة على تنبيه وارسال إشارة إلى النباتات

, باإلضافة فطريات الميكوريزا من هجوم وشيك , مما يمنحها انذاراّ مبكراّ والوقت الكافى لتكوين دفاعاتها الكيميائية الخاصة من أجل منع اإلصابة . إلى تحذير النباتات المجاورة من هجمات أكالت األعشاب , ومسببات األمراض والجفاف الوشيك

للقبض على فرائسها من النيماتودا والتغذية مصائد متخصصة nematophagous fungiتستخدم الفطريات المتغذية على النيماتودا و ل نمط عليها نتيجة تأثير فيرمونات األسكاروسيدات , والتي تفرز بشكل جوهري من قبل العديد من أنواع النيماتودا التي تعيش في التربة , وتمث

عرف مكان ت على الفريسة وتكوين التراكيب الصائدة المناسبة , وهى بذلك eavesdropجزيئي تستخدمه الفطريات المفترسة للنيماتودا للتنصت أكثر دقة من وصف التواصل. " التنصت", هذا مما دفع بعض الباحثين إلى أن وصف من النيماتودا اوجود فرائسه

لديه مقدرة على اكتشاف أقصر الطرق الى الغذاء من خالل Physarum polycephalum كما أظهرت الدراسات أن فطر العفن الهالمي متاهة من الممرات حيث وضع الباحثون الغذاء عند مخرج كل نفق من المتاهة ووضعوا الفطر في بداية المتاهة وتركوه يبحث عن أقصر الطرق

بداية أرسل الفطر خيوط استشعار دقيقة الى كل االتجاهات حتى امتلئت جميع الممرات بخيوط االستشعار, اال أن اثنين من الخيوط بنفسه . في التمكنا من لمس الغذاء مبكرا من طرق مختصرة وارسال على الفور موجات من التقلصات )المعلومات( الى بقية جسم الفطر, هذه االشارة جعلت

شعار تتقلص وتختفي, في حين أن خيط االستشعار الذي وجد الغذاء مبكرا أصبح اكثر سمكا, وبعد ثمانية ساعات أصبح الفطر بقية خيوط االست الهالمى شريطا واحدا يتصل طرفيه بالغذاء من أقصر الطرق.

يابانية طوكيو, حيث الموقع المستقبلى وفى تجربة وضع فطر العفن الهالمى على سطح بيئة غذائية تحاكى نموذج لخريطة لموقع العاصمة ال نما فطر العفن الهالمى خارجاّ من مصدر الغذاء األولي واستعمر كل من المصادر الغذائية األخرى , , و لغيرها من المدن الرئيسية في المنطقة

عن طريق ربط المصادر الغذائية ببعضها المساراتوفي نهاية المطاف , فإن الفطر حل مشكلة ربط العاصمة بالمدن الرئيسية عن طريق شبكة من على المدن الرئيسية في البالد, ووضع فطر العفن على موقع تم استعمال خريطة لكندا, ووضع قليل من رقائق الشوفان أخرى وفي تجربة .البعض

كفاءة إلى المدن األخرى , وفى خالل , وذلك تحت ظروف مناسبة من الحرارة والرطوبة , وتركوا الفطر يشكل المسارات األكثر مدينة تورونتو . ساعات كّون الفطر شبكات من الطرق تكاد تحاكي تماما نظام الطرق الكندية السريعة الفعلية

, وكانت الفكرة هي البحث عن طرق أبسط للسيطرة على P. polycephalumوكذلك طور العلماء روبوت يتحكم فيه فطر العفن الهالمى ال يتم التحكم فيها عن طريق األسالك أو الترانزستورات, sensor chip, وهناك دراسة أخرى أختبرت فيه رقاقة استشعار تسلوك الروبوتا

يمكنها معالجة المعلومات بصورة أفضل, كما hybrid technologyولكن عن طريق فطر العفن الهالمى , ويعتقد أن هذه التكنولوجيا الهجينة أن القدرة على التعلم والنمو لفطر العفن الهالمى من خالل الخبرات والتجربة والخطأ سوف يجعل من الممكن حل المشاكل في كل من علم

.P على الفطر , ويعتقد العلماء أن بحوث الحوسبة القائمة computer scienceوعلم الحاسوب neuroscienceاألعصاب polycephalum ستؤدي إلى ثورة في صناعة اإللكترونيات البيولوجية والكمبيوترrevolution in the bioelectronics and

computer industry , . وهذا يمثل خطوة هامة نحو استخدام أكثر انتشارا لمكونات وأجهزة يتم التحكم فيها بيولوجيا



23

O14 ROLE OF TRICHODERMA VOLATILES IN SOIL

Metwally R. Kottb



Several fungi produce during primary and secondary metabolism, various mixtures of gas-phase, carbon-based

compounds called volatile organic compounds (VOCs). Due to their small size, they are able to diffuse easily

through the atmosphere and soils. These compounds are biologically important by being involved in different

ecological interactions including, those between fungi and plants, arthropods, bacteria, and other fungi. Fungal

VOCs can be used in various biotechnological applications e.g. biofuel, biocontrol, mycotaxonomy and disease

diagnosis. Among those fungi developing VOCs, the genus Trichoderma might be included as one of the most

active. The production ability of VOCs differs markedly from one species to another within the same genus.

The chemistry profile of VOCs of several isolates of Trichoderma, isolated from different Egyptian habitats, was

determined where several chemical classes were detected and quantified. These include: pyrones, sesquiterpens,

alcohols and alkans, in addition to CO2 were identified and quantified. One isolate (identified by molecular tools

as T. asperellum) was selected as it was able to produce extraordinary high amount of 6-pentyl-α-pyrone. The

effect of Trichoderma VOCs on plant growth and triggering of plant defence was tested. The results clearly

showed that VOCs of T. asperellum revealed strong ability toward the modulation of plant growth and induction

of defence signals which have been detected at the biochemical and molecular levels.

A test for the antifungal activity of VOCs produced by different Trichoderma spp, showed that T. harzianum

came first followed by T. asperellum and Trichoderma sp1. The strong effect revealed by T. harzianum was

attributed to the types of VOCs produced where the following compounds were detected: Acora 3,5 dien11ol,

Trichoacorenol, Phenyl ethyl alcohol. Similarly, a test for nematicidal activity of VOCs against cereal cyst

nematodes “Heterodera avenae” showed that VOCs of T. asperellum significantly inhibit nematode mobility and

egg hatching. In conclusion, VOCs of Trichoderma could be a promising tool in biological control as it showed

strong interaction with plants, fungi and nematodes.


24

O15 ISOLATION AND IDENTIFICATION OF FUNGI AND THEIR MYCOTOXINS FROM MILK

Ismail Seddik Mohamed1, Mohamed Wael Abd-Alazeem

2, Serageldeen Sultan

2 and Hams Mohamed

Ahmed2

1Department of Microbiology and Immunology, Faculty of Medicine, Assuit University;

2Department of

Microbiology, Faculty of Veterinary Medicine, South Valley University, Egypt.


A total of 50 milk samples from cows and buffaloes (25 samples for each) were randomly collected and subjected

to mycological and mycotoxicological evaluation. The mean total yeast counts (colonies/gm) were 2.4x107 and

1.1x107 for cow and buffalo milk respectively, while the mean total mould counts (colonies/gm) were 6.7x10

2

and 1.6x104 for cow milk and buffalo milk respectively. The common yeasts isolated from cow and buffalo milk

included Candida spp. and Kluveromyces marxianus. The predominant species of moulds were Aspergillus niger

and A. flavus which were isolated from the examined samples at varying percentages ranged from 12-24%.

Isolates of moulds were screened for mycotoxins production by TLC method, Ochratoxin was extracted from A.

niger isolates from cow and buffalo milk while aflatoxin was only extracted from A. flavus isolated from buffalo

milk.


25

O16 THREATING MYCOTOXINS IN THE URINE OF EWES IN UPPER EGYPT

Amal Mokhtar1 and Ahmed M. Ali

2

1Microbiology and immunology Department, Faculty of Veterinary Medicine, Aswan University;

2Chemistry

Department, Faculty of Science, Al-Azhar University – Assiut Branch


Mycotoxins are very harmful environmental contaminants for human being and his animal wealth. Mycotoxins of

many genera could be detected in the animal meat, eggs, milk and dairy products. Exposed animals to the

mycotoxins of Aspergillus flavus and Fusarium are commonly concerned.

In this work Aflatoxin B1, and its metabolites as well as Zearalenone (or Zearalenol) and Ochratoxin were

detected in randomly 24 hours collected urine of 28 ewes (2-5 years old) from Toshka (Aswan governerate).

The urine samples were incubated with active glucuronidase to liberate the toxins from the conjugated

glucuronides. The predicted liberated toxine and their metabolites were extracted in the organic solvents to be

chromatographed on TLC precoated plates.

Aflatoxin B1 and some metabolites such as AFM1 and AFQ1 were detected (according to their RF values in

chloroform: methanol 97:3) on silica gel in five samples.

Noticeably, 22 from the tested 28 cases were exposed to the Zearlenone while only one case with Ochratoxin A

were observed.

Conclusively the exposure of ewes to the Fusarium mycotoxin Zearalenone is more common in the Upper Egypt

than the others. Such a conclusion can interpret that 2 ewes of these tested 28 ones were subjected to abortion

since the estrogenic effect of Zearelenone is a causative factor for such a defect.



26

O17 NATURAL OCCURRENCE OF FUSARIUM MYCOTOXINS (FUMONISINS, ZEARALENONE

AND T-2 TOXIN) IN CORN FOR HUMAN CONSUMPTION, YEMEN

Hala Jameel Al-Jebouri, Ahmed Lotfy E. Mahmoud and Abdullah Yahya Al-Mahdi

Biology Department, Faculty of Science, Sana’a University, Yemen.


The presence of fumonisins, zearalenone (ZEA) and T-2 toxin has not been regulated in the legislation of

Republic of Yemen. Therefore, the data on contamination of cereals, especially corn, which is highly susceptible

to contamination by these toxins, are not sufficient. In this regard, fifty samples of corn kernels which showed

Fusarium contamination were collected from different corn growing areas in Yemen (Ibb, Taiz, Dhamar, Sana’a

and Al-Hodaida), during the spring- summer (2010 – 2011 and 2011 – 2012) cycles, and were further analyzed

for the presence of these toxins using thin layer chromatography (TLC) and enzyme – linked immunosorbent

assay (ELISA) techniques. Of the tested samples 72% were contaminated with fumonisins at concentrations

ranging from 00.57 to 120.20 µg/g with an average of 9.33 µg/g. Only 30% of samples were contaminated with

ZEA with concentrations ranging from 00.17-03.30 µg/g with an average of 01.04 µg/g. Fortunately, all samples

were free of T-2 toxin. The co-occurrence of fumonisins and ZEA was also detected in 15 samples (30%). This

study is considered the first preliminary survey dealing with three mycotoxins occurring naturally in fresh corn in

Yemen. It is highly recommended to use this study as a base for further investigations on other cereals. The high

levels of fumonisins and ZEA can cause over mycotoxicoses and impairment of the immune system in animals

consuming corn kernels contaminated with these harmful mycotoxins.


27

O18 ROLE OF INDOOR AND OUTDOOR AIRBORNE FUNGI IN THE DEVELOPMENT OF

RESPIRATORY ALLERGIC DISEASES IN SAUDI ARABIA

Syed M Hasnain1 and Abdulrahman S. Alfrayh

2

1Department of Cell Biology, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia,

2Department of Pediatrics, College of Medicine, King Saud University, Riyadh, Saudi Arabia.


The role of fungi and their airborne spores are well known in the sensitization of susceptible human individuals

and thereby initiating the development of allergic reactions. Bronchial asthma, allergic rhinitis, rhino-

conjunctivitis, conjunctivitis and allergic broncho-pulmonary aspergillosis are certain human diseases commonly

associated with Fungi. These are in addition to well- known mycological diseases in human including

aspergilloma where fungi are known to infect, colonize and affect human by mycotoxins and mycotoxicosis.

In Saudi Arabia where some of the allergic diseases, particularly bronchial asthma, are on the rise, airborne

monitoring and detection of indoor and outdoor fungal spores has been undertaken using both viable and non-

viable methods. A number of fungal spores were identified in different parts of the country, major cities and

homes etc. A relationship between the various identified fungi were demonstrated by IgE mediated skin prick

testing (SPT) on allergic patients coming to allergy clinics of several hospitals. The five most dominant fungi

identified, both indoor and outdoor with some degree of variations, were Cladosporium cladosporioides,

Cladosporium herbarum, Alternaria alternata, Aspergillus fumigatus and Penicillium chrysogenum. In addition,

Ganoderma mastoporum and various other basidiospores and ascospores such as Leptosphaeria were also

identified outdoor on non-viable trap slides. Because of monitoring methodology in most part of the world,

basidiospores and ascospores remain undetected. This may be one of the reasons that the allergenicity to

basidiospoesis limited to some western countries of the world.

In this presentation, aero-mycological or medical aerobiology data as well as clinical data from Saudi Arabia will

be discussed in relation to the prevalent allergic diseases. It is expected that the discussion will provide an

opportunity for collaboration between mycologists and allergists and generate interest amongst mycologists to

develop the speciality in this part of the world.



28

O19 UNDERSTANDING DANDRUFF: ROLE OF FUNGI

Sahar Abdelomez Ismail

Department of Dermatology, Venereology and Andrology, Faculty of Medicine, Assiut University, Egypt.


Dandruff is a common scalp disorder affecting almost half of the population at the post-pubertal age. Although

dandruff isn't contagious and is rarely serious, it can be embarrassing and sometimes difficult to treat. The

etiology of dandruff depends upon three factors: individual susceptibility, sebaceous gland secretions, and

microfloral metabolism. The vast majority of recent data supports a direct causal link between Malassezia fungi

and dandruff.


29

O20 PRESENCE OF FUNGI IN SCALP OF PATIENTS WITH ALOPECIA AREATA: TRIGGERING

FACTOR OR CO-EXISTANCE

Hisham D Gaber¹, Ahmed M 2 Moharram and Reham M Abdel Gaber¹.

¹Department of Dermatology, Venereology and Andrology, Faculty of Medicine, Assiut University, Egypt; 2Department of Botany and Microbiology, Faculty of Science, Assiut University, Egypt.


Background: Alopecia areata (AA) is the most frequent cause of inflammation-induced hair loss, affecting 0.1

to 0.2% of population worldwide. Despite its prevalence, there is little known about the underlying etiology and

pathogenesis. Several data indicate a role of genetic susceptibility to develop the disease. Multiple candidate

genes have been suggested. It has been suggested that hair follicle melanocytes, dermal papilla cells, or

keratinocytes may contain the triggering antigens Viral, bacterial or fungal pathogens have been implied as

possible triggering factors of autoimmune reactions. Little is known about the pathogenic mechanisms by which

these fungi cause disease, particularly in immunocompetent individuals.

Methodology: Thirty patients diagnosed clinically as alopecia areata and 30 normal age matched

persons has undergone mycological examination. Mycological culture of epidermal scrapings from the

scalp was done.

Results: In alopecia group, 36.7% (n: 11) showed positive results by DME but only 20% (n: 6)

revealed positive culture on Sabouraud`s agar were observed. However, among the control group

26.7% (n: 8) showed positive results by DME, only 13% (n: 4) of them gave positive culture on

Sabouraud`s agar.

Conclusion: the possible role of fungal antigens (eg. antigens involved in melanin synthesis) in

triggering autoimmunity in alopecia areata still needs further research on a wider scale of cases.


30

O21 MYCOLOGICAL STUDIES ON DERMATOMYCOTIC DISEASES IN TAIZ CITY, YEMEN

Ali M. H. Sallam

Department of Microbiology, Faculty of Science, Taiz University, Taiz, Yemen.


This Study aimed at investigating the distribution of dermatomycoses and diversity of dermatophytes associated

with the human skin infection at Taiz City, Yemen. The study extended from May 2013–May 2014 using

Sabouraud's dextrose agar (SDA) at 28ºC. Results showed that males were more affected than females. The direct

microscopic examination indicated that about 71% of the cases were positive. Tinea versicolor was the

commonest dermatomycotic disease (45 out of 120 cases). The most common dermatophytes were Trichophyton

violaceum, T. rubrum and Microsporum canis.


31

O22 ECOLOGICAL STUDIES ON CRYPTOCOCCUS SPECIES INHABITANT IN THE SUDAN

Nadir M. K. Abuzeid1, Mohmoud Bashir

1, Eltaib Abdallah

1, Abdallah Bashir

1, Abdalmajid Mohammed

1,

Mohammed Yousif1, Elmahi Mohammed1, Abdallah Adam

2, Awatif Eltayeb

3, Elham Abelbaseit

4, Yousif

Tybin5.

1Omdurman Islamic University, Faculty of Medical laboratory Science, Department of Clinical Microbiology,

P.o.x:382 Omdurman Sudan.2Omdurman Islamic University, Faculty of Sciences and Biotechnology,

Department of Microbiology, P.o.x:382 Omdurman Sudan.3Khartoum University, Faculty of Sciences,

Department of Botany, Khartoum, Sudan.4National Research Centre, Veterinary Medicine, Department of

Mycology, Khartoum-Sudan.5Bahary University, Faculty of Medicine, Department of Dermatology, Bahary,

Sudan.


Cryptococcosis has emerged as an important public health problem in Africa, Asia and the Americas due to the

increasing numbers of persons at risk of this infection and the adaptation of its a etiological agents to new

environments. Cryptococcus belongs to the Phylum Basidiomycota and can be readily distinguished from other

pathogenic yeasts such as Candida by the presence of a polysaccharide capsule, formation of melanin, and urease

activity, which all function as virulence determinants. This study was designed to determine the phenotypes of

Cryptococcus species and characterization of their environmental sources. A total of 572 environmental samples

were collected from different sources (faeces, soil, milk, water, air and plants) at Khartoum State. Various

techniques were conducted for culturing on Sabouraud`s dextrose agar (SDA), according to the type of sample.

Isolated yeasts were identified according to their capsules stained by India ink, morphology, growth conditions on

different media and biochemical tests. Results revealed that the best dominant niche for inhabitant Cryptococcus

species is pigeon faeces compared with other sources. C. neoformans, C. laurentii, C. albidus and C. terreus were

the species that are ubiquitous to the bird dung with high incidence in clay cages that protected from sunshine and

acidic pH. The C. laurentii was the most common isolated.



32

O23 MYCOTIC INFECTIONS IN DIABETIC FOOT PATIENTS

Ahmad Mohammad Moharram1, Ismail S. Mohamed

2, Ibrahim M. A.

2 and Fatma Sayed Abdel-Sameii

2

1Department of Botany and Microbiology, Faculty of Science, Assiut University, Egypt;

2Department of

Microbiology and Immunology, Faculty of Medicine, Assiut University, Egypt.


A hospital based study was conducted over a period of 15 months from March 2013 to May 2014 and included

130 patients (72 males and 58 females) complaining of diabetic foot ulcer admitted to the Vascular Surgery

Department. The age of patients ranged from 45-70 years. Fungal infection was diagnosed by both direct

micrsoscopic examination of swab samples and culturing on Sabouraud`s dextrose agar. Seven yeast species were

isolated and identified, namely Candida albicans (11.11% of the samples), C. tropicalis (27.77%), C. glabrata

(25.92%), C. krusei (12.96%), C. parapsilosis (16.66%), C. caryicola (3.7%) and Kodamaea ohmeri (1.85%).

Filamentous fungi were represented by Aspergillus flavus, A. fumigatus, A. niger, Rhizopus stolonifer and

Syncephalastrum racemosum. The antifungal susceptibility pattern of 54 yeast isolates showed their highest

sensitivity to Fluconazole (68.5% of the total isolates) followed by Amphotericin B (68.4%), while most of

isolates were resistant to Itraconazole (96.3%) and Clotrimazole (75.9%). Aspergillus species showed high

sensitivity to Terbinafine (100%) followed by clove oil (60%), while most of the isolates were resistant to

Itraconazole (95%) followed by Amphotericin B (90%). Isolates of Rhizopus stolonifer were only sensitive to

clove oil and Terbinafine.

Syncephalastrum racemosum showed sensitivity to Amphotericin B and clove oil and resistance to the rest of

antifungal agents



33

O24 SOME IMPORTANT PHARMACEUTIC COMPOUNDS PRODUCED BY FUNGI

Abdel-Naser A. Zohri

Botany & Microbiology Department, Faculty of Science, Assiut University, Egypt.


The natural products from microbial origin have played and still play an invaluable role in drug discovery.

Microbial production of drugs used to prevent or treat a variety of diseases received a lot of attention.

Development of microbial products with new medicinal activities has turned out to be a large research area.

Pharmaceutical research has exploited ergosterol as a target for such antimycotic drug as amphotericin B. Also, it

acts as a precursor of vitamins. Cyclosporins are powerful drugs in human transplantation surgery to prevent

rejection of transplanted organs. Also, these drugs had antiparasitic, fungicidal, immunosuppressive and chronic

antiinflammatory activities. Lovastatin (mevacor) is a hypocholesterolmic drug. Also, it is used for the treatment

of various tumor types. ß-glucans exhibit medicinal properties such as antitumor, antimicrobial and antioxidant

activities plus mycotoxinabsorptionas well as uses in stimulation of the immune response and the reduction of

blood cholesterol and glucose levels. Steroid drugs and hormones are very important therapeutic compounds.

11β-hydroxy-testosterone is a strong anti-tumor drug against human ovarian cancer and having anti-

hypercholesterolemic properties. Fungal production of ergosterol, cyclosporin A, lovastatin and ß-glucans as very

important pharmaceutical compounds are reviewed. Fungal transformations of steroids with special reference to

11β-hydroxy-testosterone formation are also included. Optimizations of both nutritional and environmental

factors affecting the production of these pharmaceutical compounds are discussed.


34

O25 MACROFUNGI AS A PRECIOUS SOURCE OF PHARMACEUTICAL COMPOUNDS.

Ahmad Mohammad Moharram

Department of Botany and Microbiology, Faculty of Science, Assiut University, Egypt


Macrofungi including medicinal mushrooms, morels, truffles and others have been reported to produce at least

126 bioactive compounds. Agaricus bisporus contains high levels of dietary fibers and antioxidants including

vitamin C, D, and B12; folates; and polyphenols that may provide beneficial effects on cardiovascular and

diabetic diseases. Agaricus extract contains more beta glucans than any other mushroom species investigated.

Cordyceps sinensis acts an immune stimulator by raising cancer- and virus-fighting T Cells and Natural Killer

Cells and prolongs the life of white blood cells. It has demonstrated anti-tumor properties and also protects the

kidneys from chemotherapy side effects. A study on the nucleoside antagonist, Cordycepin shows that it causes

double strand breaks in the DNA of breast cancer cells. Several oriental studies have shown Ganoderma lucidum

to have an inhibiting effect on the growth of tumor cells of the lungs, cervix, prostate, liver, and on sarcomas and

leukemia. This effect was suggested to be due to stimulating the body's own cancer fighting mechanisms by

increasing the activity of T-lymphocytes, and interleukin-1 and 6. Therapeutic effects of mycelia extracts from

Grifola frondosa (Maitake) include: anti-fungal (Candida albicans) and anti-bacterial, blood pressure moderator,

nerve tonic, respiratory conditions, and stress reduction. Lion's Mane Mushroom is known in the wild from North

America, Europe, China and Japan. It grows on many species of deciduous trees, in particular oak, walnut,

beech, maple and sycamore. The active compounds that have received the most attention for their nerve

regenerating properties are named erinacines, a series of low molecular weight molecules that are able to pass

through the blood brain barrier - an essential attribute for any medicinal compound expected to be able to effect

healing on myelin or nerve tissue. Other research studies on Lion's Mane Mushroom extract have shown it to

possess the following properties: Immune stimulating, antimicrobial against Aspergillus, Candida, and bacilli and

anti-tumoral. Compounds from other medicinal mushrooms with proven anti-cancer and antimicrobial properties

have been studied in pre-clinical models and will increasingly be submitted for clinical trials.


http://www.ncbi.nlm.nih.gov/pubmed/22821173


35

O26 BIOGENIC SILVER NANOPARTICLES OF RESISTANT ASPERGILLUS FLAVUS AUMC 9834

AGAINST SOME PATHOGENIC MICROORGANISMS AND THEIR SYNERGISTIC

EFFECT WITH ANTIBIOTIC FLUCONAZOLE.

Ahmed A. Shoreit, Mady A. Ismail, Ahmad M. Moharram and Mohammad H. Abdullah



The aim of this study was to biosynthesis silver nanoparticles (AgNPs) from Aspergillus flavus AUMC 9834

isolated from oral suspension of beta-lactam antibiotic Cefpodoximeproxetil, its antimicrobial activity, and the

synergistic effect of AgNPs in combination with the commonly used antifungal fluconazole against a resistant

fungus. The synthesized AgNPs from cell free filtrate were characterized using UV-VS spectrophotometer

analysis, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and transmission electron

microscopy (TEM). UV-VS spectrophotometer analysis showed a peak at 420 nm corresponding to the Plasmon

absorbance of silver nanoparticles and FTIR analysis showed the potential biomolecule responsible for the

reduction of silver. The structural properties of silver nanoparticles were confirmed using XRD technique, while

TEM micrographs revealed that the silver nanoparticles are dispersed and aggregated, and mostly having

spherical shape measuring 5 - 19 nm. The synthesized silver nanoparticles plus fungal filtrate exhibited a varied

growth inhibition activity against the tested pathogenic bacteria. A significant increase in area of growth

inhibition was observed when a combination of silver nanoparticles and fluconazole was applied. The current

results revealed that the synthesized silver nanoparticles produced by the fungus A. flavus is promising to be used

in medical therapy due to their broad spectrum against some pathogenic bacteria and resistant tested fungus.



36

O27 ROLE OF BIOTECHNOLOGY IN PRODUCTION OF HBV VACCINE BY SACCHAROMYCES

CEREVISIAE

Naeima M. H. Yousef

Department of Botany and Microbiology, Faculty of Science, Assiut University, 71516, Egypt


Hepatitis B is a serious disease caused by the hepatitis B virus (HBV). Infection with this virus can cause scarring

of the liver, liver failure, liver cancer, and even death. Hepatitis B virus is spherical in shape with a diameter of 42

nm, DNA core enclosed within a lipoprotein coat. Hepatitis B vaccine is the first recombinant vaccine developed

for the prevention of hepatitis B virus infection. The vaccine contains one of the viral envelope proteins, hepatitis

B surface antigen (HBsAg). It is produced by yeast cells, into which the genetic code for HBsAg has been

inserted. This type of vaccine called also subunit vaccine against hepatitis B virus, which is composed of only the

surface proteins of the virus. Recombinant vaccine produced using an expression plasmid vector. The target gene

of interest is cloned into the expression vector and then transfected into host cells. Once inside the cells, the

expression vector uses the cell’s machinery for transcription to produce mRNA, and then the translational

machinery to produce the protein of interest. After expression of the gene product in cells, the target protein must

be purified from the proteins of the host cell, then purified and package.

—

http://www.webmd.com/hepatitis/default.htm

http://www.webmd.com/content/article/90/100597.htm

http://www.webmd.com/cancer/understanding-liver-cancer-basic-information

http://en.wikipedia.org/wiki/Vaccine

http://en.wikipedia.org/wiki/Hepatitis_B

http://en.wikipedia.org/wiki/Proteins

http://en.wikipedia.org/wiki/HBsAg


37

Abstracts of

POSTER PRESENTATIONS


38

P1 ILYONECTRIA RADICICOLA SPECIES COMPLEX FROM PANAX GINSENG

MEYER AND THEIR PATHOGENICITY.

Mohamed El-Agamy Farh, Johan Sukweenadhi, Md-AmdadulHuq, Yeon-Ju Kim, Deok-Chun Yang Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University Giheung-gu Yongin-si,

Gyeonggi-do, Republic of Korea.


Ilyonectria radicicola (previously named Cylindrocarpon destructans) is a soil borne pathogen that causes

diverse root diseases to many hosts including Panax ginseng. Strains of I. radicicola still show morphological as

well as pathological diversity. Previous studies proved the polyphyletic properties of I. radicicola species

complex based on the molecular sequences; internal transcribed spacer, β-tubulin, translation elongation α-1 and

particularly histone H3 and up to now new species are being discovered. Strains that infect P. ginseng are not

clearly identified yet. On the other hand, it was found that I. radicicola was able to deglycosylate P. ginseng's

saponins, Protopanaxadiol (PPD) type ginsenosides, Rb1, Rb2, Rc and Rd. This activity was proven to be

correlated with the development of damping off caused by pythium irregulare in ginseng seedling. The main

reason for the disease development by I. radicicola is still unclear. Therefore, our work aims to clarify the factor

responsible for disease development by I. radicicola species complex; genetic diversity or ginsenosides

deglycosylation. Fourteen I. radicicola strains were obtained from the Asian culture collection; KACC, NBRC,

and isolated from infected roots. Based on the previous studies, we identified the strains using histone H3

sequence. On the other hand, the interaction of the strains with the PPD type ginsenosides were also checked

through the measurement of the growth rate of the strains and hydrolysis rate of PPD type ginsenosides.

Interestingly the phylogenetic analysis of the strains and their interaction with PPD type ginsenosides did not give

correlated results. We are doing ex vivo pathogenicity test to confirm the main factor for causing infection.


39

P2 PREVALENCE OF DERMATOPHYTOSES IN DHAMAR CITY, YEMEN

Najla M. Baghza

Department of Microbiology, Faculty of Medicine and Health Sciences, Dhamar University, Dhamar, Yemen

E-mail: baghza2006 @yahoo.com

The prevalence of dermatomycosis infection among human in Dhamar City, Yemen, was investigated. Two

hundred and fifty specimens (scales, hair and nails) were collected from patients suffering of dermatophytoses.

Tinea manuum was the most clinical type distribution of dermatophytoses. The mycological analysis of the

collected specimens revealed that Trichophyton soudanens and Microsporum cookei were the most frequent

isolated dermatophytes. Screening of the isolated dermatophytes for their resistance to antifungal compounds

revealed that Ketoconazole was the most effective. The relations, between prevalence of dermatophytoses and

each of age, sex, level of education, exposure to animal and place of residence of patients were also discussed.


40

P3 ECO-FRIENDLY BIONEMATICIDES AGAINST MELOIDOGYNE JAVANICA

INFECTING OKRA PLANTS

S. A. Montasser1, M.A. Ahmed

2, A. F. El-mesalamy

2 and Atef M. El-sagheer

2

1Agriculyure, Zoology and Nematology Department, Faculty of Agriculture Al-Azhar University, Cairo, Egypt,

2Agriculyure, Zoology and Nematology Department, Faculty of Agriculture Al-Azhar University, Assiut, Egypt.


Compounds of biological asset are considered as new efficient method that becomes widely used for controlling

plant parasitic nematodes, as aim to decrease the extent of environment degradation and the chemical effect of

nematicides. So six bionematicides were evaluated in controlling the root-knot nematode Meloidogyne javanica

infecting okra plants in three application times and two concentrationsunder greenhouse conditions. All the tested

bionematicides significantly reduced the numbers of nematodes in soil, galls formation and egg-masses than those

in the check. Agrein at low concentration, and Bioark and Biozayd at high concentration in simultaneous

treatment of the nematicide and inoculation of the nematode proved to be the best effects on nematode control.

On the other hand, all tasted bionematicides significantly succeeded to improve the plant growth as compared to

the check. Highly significant increase in weights of both shoot and roots of plants grown in soil treated with

Agrein, Bioark and Biozayd at tow concentrations in simultaneously treatment as compared to different time of

the nematicide and the nematode application.



41

P4 PRODUCTION OF KOJIC ACID BY ENDOPHYTIC FUNGI ISOLATED FROM

MEDICINAL PLANT IN EGYPT

Nermien H. Seddek


Two hundred and fourteen endophytic fungal isolates collected from apparently healthy leaves of eleven common

medicinal plants in Egypt were screened for production of kojic acid using static cultivation at 28 °C. Twenty

four endophytic fungal isolates showed the ability to produce kojic acid; these isolates belong to Aspergillus (five

isolates of two species and two species varieties), Petromyces (nine isolates of two species), Penicillium (six

isolates of four species), Chaetomium globosum (two isolates) and one isolate from each of Emericella nidulans

and Pleospora allii. High levels of kojic acid (≥ 12 g/l) were produced by six fungal isolates belonging to the

genera Petromyces and Aspergillus. Three isolates of Petromyces flavus originated from Datura stramonium,

Hyoscyamus muticus and Withania somnifera were able to produce 20.68 ± 0.03, 20.9 ± 0.59 and 23.84 ± 0.1 g/l

of kojic acid, respectively. One isolate of P. parasiticus No. 63 collected from Cyperus rotundus produced 17.71

± 0.19 g/l of kojic acid. The remaining two isolates were Aspergillus flavus var. columnaris isolated from

Rosmarinus officinalis and Zizyphus spina-christi which produced 19.18 ± 0.72 and 19.52 ± 0.47 g/l of kojic acid,

respectively.


42

POSTER PRESENTATION [P-5]

BIODIVERSITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH EGYPTIAN

DATURA

Nermien H. Seddek


Endophytic fungi are specific microbes that live at a specific ecosystem in nature. Various endophytic fungi have

been known possess an ability to produce a broad range of biologically active substances. Totally 26 endophytic

filamentous fungi were isolated from healthy leaves of two species of Datura (D. innoxia and D. stramonium)

cultivated in Egypt. Out of 1350 sterilized investigated segments, endophytic fungi colonized 858 segments from

two tested Datura species on three types of media contributing 63.56% colonization frequency. Only Alternaria

alternata and Aspergillus japonicus showed spatial heterogeneity and host recurrence on this work but all

endophytes appeared on each plant species showed host specificity.


43

LIST OF PARTICIPANTS


44

Abd-Alazeem, Mohamed Wael

Department of Microbiology,

Faculty of Veterinary Medicine,

South Valley University

Telephone:

E-mail:

Abdallah, Eltaib

Department of Clinical Microbiology,

Faculty of Medical laboratory Science,

Omdurman Islamic University,

P.o.x:382 Omdurman Sudan.

Telephone:

E-mail:

Abdelbaseit, Elham

Department of Mycology,

National Research Centre,

Veterinary Medicine,

Khartoum-Sudan.

Telephone:

E-mail:

Abd-Alla, Mohamed

Department of Plant Pathology,

Faculty of Agriculture,

Assiut University,

Telephone:

E-mail:

Abd-Ella, Aly Ahmed

Department of Plant Protection,


Assiut University,

Telephone: 01142269247


Abdel-Azeem, Nagwa E.

Department of Dermatology,

Faculty of Medicine,

Assiut University,


E-mail:

Abdel-Sameii, Fatma Sayed

Department of Microbiology and Immunology,


Assiut University,

Egypt.

Telephone:

E-mail:

Abdel-Rahim, Mohamed H.



Assiut University,

Telephone:

E-mail:

Abdel-Rahman, Mohamed A. A.

Plant Protection Research Institute,

Agricultural Research Center

Telephone:

E-mail:

Abdel-Galil Farouk A.



Alexandria University

Telephone:

E-mail:

Abdel Sater, Mohammed A.

Department of Botany and Microbiology,

Faculty of Science,

Assiut University,



Abdel-Razik Abdel-Aleem

Department of Plant Diseases,


Faculty University

Telephone:

E-mail:

Abdullah, Mohamed Hassan

T3A Factory, Assiut


Fax:


Abdullah, Qais Y. M.

Department of Biology,

Faculty of Science,

University of Sana'a,

Republic of Yemen

Telephone:

E-mail:




45

Abdu-Allah, Gamal Abdel-Latif Mohamed



Assiut University



Abed, Rabab Majead


College of Education for Pure Science,

Diyala University,

Diyala - Iraq

Telephone:


Abo El-Kassem, Nabil

Botany Department,

Fayoum University,

Fayoum, Egypt

Telephone:

E-mail:

Abou-Gadeer, M. F.



Assiut University



Abou-Gadir, Nesreen M. F.



Assiut University



Aboud, Hadi Mahdi

Directorate of Agriculture Research

Ministry of Science and Technology

Baghdad - Iraq

Telephone:

E-mail:

Abou El-Soud, Mohamed Saad



Alexandria University

Telephone:

E-mail:

Abu El-Naga, Heidi Ibrahim Gabr



Assiut University,


E-mail: heidiaboalnaga1 @yahoo.com

Abu El-Yusr Amal Ibrahim



Assiut University

Telephone:

E-mail:

Abuzeid, Nadir

Department of Medical Microbiology,

Faculty of Medical Laboratory Sciences,

Omdurman Islamic University (OIU)

P.O. Box: 382.Omdurman-Sudan

Telephone: 00249-121072496

Fax : 00249-183-786085


Adam, Abdallah


Faculty of Sciences and Biotechnology,



Telephone:

E-mail:

Ahmed, Hams Mohamed

Department of Microbiology ,



Qena, Egypt



Ahmed, Amer Fayez



Assiut University

Telephone:

E-mail:

Ahmed, Hoda A. M.

Plant Pathology Research Institute,

Agricultural Research Center,

Giza, Egypt


E-mail:[email protected]





46

Ahmed M. Ali

Department of Chemistry,

Faculty of Science,

Al-Azhar University – Assiut Branch



Ahmed M. Ali



Ain Shams University

Telephone:

E-mail:

Ahmed, M. A.

Department of Agriculture Zool. and Nematol.,


Al-Azhar University,

Assiut, Egypt

Telephone:

E-mail:

Ahmed, Mohammed Sayed


Assiut University,

Telephone:

E-mail:

Ahmed, Sayed Ashour



Assiut University

Telephone:

E-mail:

Al-Bedak, O. A

Assiut University Mycological Centre,

Faculty of Science,

Assiut University,

71516 Assiut, Egypt



Alfrayh, Abdulrahman S.

MD, FACAAI, FAAAAI

Department of Pediatrics,

College of Medicine,

King Saud University,

Riyadh, Saudi Arabia

Telephone:


Al-Eraky Amal Ibrahim



Assiut University

Telephone:

E-mail:

Ali, Mai

Botany Department,

Fayoum University,

Fayoum, Egypt

Telephone:

E-mail:

Ali, Refat

Botany Department,

Fayoum University,

Fayoum, Egypt

Telephone:

E-mail:

Al-Araby, Ali Amer

Department of Botany,

Faculty of Science,

Algabel Algharbi University,

Libya

Telephone:

E-mail:

Alghalibi, Saeed M.S.


Faculty of Science,

Sana’a University,

Yemen

Telephone: 00967-733583112

Fax: 00967-210105


Al-Jebouri, Hala Jameel

Biology Department,

Faculty of Science,


Yemen

Telephone:


Al-Mahdi, Abdullah Yahya

Biology Department,

Faculty of Science,


Yemen

Telephone:

E-mail:




47

Al-Mousawi, Ali Hashim


College of Science for women,

University of Baghdad,

Baghdad - Iraq.

Telephone:

E-mail:

Al-Ryani, M. A.


Faculty of Science,

Algabel Algharbi University,

Libya



Al-Zeqry, Aida Q. M.


Faculty of Science,

University of Sana'a,

Republic of Yemen

Telephone:

E-mail:

Awad, Azza M.A.

Department of Zoology,

Faculty of Science,

Assiut University

Telephone:

E-mail:

Baghza, Najla M.


Faculty of Medicine and Health Sciences,

Damar University,

Dhamar, Yemen

Telephone:


Bashir, Abdallah


Faculty of Medical laboratory Science, Omdurman

Islamic University,

P.o.x:382 Omdurman Sudan

Telephone:

E-mail:

Bashir, Mohmoud


Faculty of Medical laboratory Science, Omdurman

Islamic University,


Telephone:

E-mail:

Darwish, Soumia Mohamed Ibrahim

Food Science & Technology Department

Faculty of Agriculture

Assiut University

Telephone: +2 - (0) 88 - 233 - 26 31

Fax: +2 - (0) 88 - 233 - 13 84

E-Mail: [email protected]

Diab, H.

Department of Dermatology, Venereology and

Andrology,


Assiut University,

Assiut, Egypt

Telephone:

E-mail:

Ellaithy, Ahmed Y. M.

National Research center,

Egypt.

Telephone:


El-Maraghy, Saad S. M. A.


Faculty of Science,

Assiut University


Fax: 0882333837


El-Mesalamy, A. F.

Department of Agric. Zool. and Nematol.,



Assiut, Egypt


E-mail:

El-Sagheer, A. M.

Department of Agric. Zool. and Nematol.,



Assiut, Egypt



Elsalahy H.


Faculty of Science,

Assiut University,

Assiut, Egypt







48

Eltayeb, Awatif


Faculty of Sciences,

Khartoum University, Khartoum-Sudan.

Telephone:

E-mail:

El-Tohamy, M. Reda



Zagazig University

Telephone:

E-mail:

El-Zawahry, Aida M.



Assiut University

Telephone:

E-mail:

El-Zemaity, Mohamed Saeed

Department of Chemical Pesticides and Poisons,


Ain Shams University,

Telephone:


Fahmy, B.F.G

Plant Protection Research Institute, Agricultural

Research Center



Farh, Mohamed El-Agamy A.

Korean Ginseng Center and Ginseng Genetic

Resource Bank,

Kyung Hee University Giheung-gu Yongin-si,


Telephone: +8201086809666/+82-31-201-2100

Fax: +82-31-202-2687

E-mail: [email protected]/[email protected]

Gouda, H. A.

Department of Botany & Microbiology,

Faculty of Science,

Assiut University,

Assiut, Egypt



Hasnain Syed M.,

Department of Cell Biology,

King Faisal Specialist Hospital and Research Centre,

Riyadh, Saudi Arabia

P.O. Box 3354, Riyadh 11211

Telephone: +966-11-5577681

Mobile: +966-508940930


Hassan, Kenawy M.



Assiut University

Telephone:

E-mail:

Hassan, Shimaa


Faculty of Science,

Assiut University



Huq, Md-Amdadul,


Resource Bank,



Telephone:

E-mail:

Hussein, Khalid A.


Faculty of Science,

Assiut University,

Telephone:

E-mail:

Hussein, Nemmat A.


Faculty of Science,

Assiut University,

Assiut, Egypt



Ibrahim M. A.



Assiut University,

Egypt.

Telephone:

E-mail:


mailto:[email protected]/[email protected]




49

Ismail, M. A.


Faculty of Science,

Assiut University,

Assiut, Egypt



Isaac, George Saad

Department of Biological and Geological Sciences

Faculty of Education

Ain Shams University


E-Mail: [email protected]

Ismail, Sahar Abdel-Moez

Department of Dermatology, Venereology and

Andrology,


Assiut University,

Assiut, Egypt

Telephone:


Kotb, Saber Abd El-Motagally H.


Assiut University,

Telephone:

E-mail:

Kottb, M.R.


Faculty of Science,

Suez Canal University,

Ismailia

Telephone:


Mahmoud, Ahmed Lotfy E.


Faculty of Science,


Yemen

Telephone:

E-mail:

Manaah, S. H.


Faculty Agriculture,

Assiut University

Telephone: 010223377214

E-mail:

Mohammed, Abdalmajid





Telephone:

E-mail:

Mohamed, Asmaa H. M.

Plant protection Research Institute,

Agricultural Research Center,

Egypt

Telephone:

E-mail:

Mohammed, Elmahi





Telephone:

E-mail:

Mohamed, Ismail Seddik



Assuit University

Telephone:

E-mail:

Mohamed, Osama Abd-El-Haq



Assiut University

Telephone:

E-mail:

Mohamed, R. A. E. O


Faculty of Science,


Tel: +201115988552

Fax: +02-088-2361152


Mohammoud, Abd El-Rady


Assiut University,

Telephone:

E-mail:





50

Moharram, A. M.


Faculty of Science,

Assiut University,

Assiut, Egypt

Telephone:


Mokhtar, Amal



Aswan University

Telephone: 01007088609/0973483104

Fax: 0973483104


Moubasher, A. H.


Faculty of Science,

Assiut University,

Assiut, Egypt


Fax: 0882361152


Moubasher, Alaa-Eldin A.



Assiut University,


E-mail:

Moubasher, Mohamed Hani

Professor of Microbiology


Faculty of Science,

University of Cairo

Giza, Egypt



Moustafa, A. F.


Faculty of Science,

Suez Canal University,

Ismailia

Telephone:

E-mail:

Montasser, S. A.

Department of Agriculture Zool. and Nematol.,



Cairo, Egypt

Telephone:

E-mail:

Nassar, Ahmed Y.

Department of Biochemistry,


Assiut University,


E-mail:

Niazi, Abdul Rehman


University of the Punjab,

Quaid-e-Azam Campus,

Lahore, Pakistan

Telephone:


Omar, Youssef M.


Faculty Agriculture,

Assiut University

Telephone:

E-mail:

Saad, Abdellah Abdel-Moniem

National Research center,

Egypt.

Telephone:

E-mail:

Sallam, A. M. H.


Faculty of Science,

Taiz University,

Taiz, Yemen

Telephone:


Sallam, Nashwa M. A.



Assiut University

Telephone:

E-mail:

Seddek, Nermien H.


Faculty of Science,

Assiut University









51

Shoreit, Ahmed A.


Faculty of Science,

Assiut University,

Assiut, Egypt

Telephone:


Sukweenadhi, Johan,


Resource Bank,



Telephone:

E-mail:

Sultan, Serageldeen


Faculty of Vet. Medicine,


Telephone:

E-mail:

Tybin, Yousif



Bahary University,

Bahary-Sudan.

Telephone:

Fax:

E-mail:

Yang, Deok-Chun


Resource Bank,



Telephone: +82-31-201-2100,

Fax: +82-31-202-2687


Yousif, Mohammed





Telephone:

E-mail:

Yousef, Naeima Mohamed Hamam


Faculty of Science,

Assiut University,



Zaki, Ahmed



Zagazig University

Telephone:

E-mail:

Zohri, Abd-El Naser A.


Faculty of Science,

Assiut University,

Telephone:

E-mail: 01006464109



المؤتمر الدولى الثانى

يقيةـيا األساسية والتطبــللميكولوج

مصر –أسيوط –جامعة أسيوط

5141مارس 41-41

جدول األعمال

ملخصات البحوثو

إتحاد الجامعات العربية

the second international conference on basic and … form of sicbam-2 book.pdf · the second...

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