the effect of artificial sugar and raw sugar on the daphnia’s heartbeat katie clarke nov. 7 2006
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The Effect of Artificial The Effect of Artificial Sugar and Raw Sugar on Sugar and Raw Sugar on the Daphnia’s Heartbeatthe Daphnia’s Heartbeat
Katie ClarkeKatie Clarke
Nov. 7 2006Nov. 7 2006
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PurposePurpose
To see how the daphnia’s To see how the daphnia’s heartbeat is affected by the heartbeat is affected by the artificial sugar and the raw sugar.artificial sugar and the raw sugar.
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Background- DaphniaBackground- Daphnia
Over 150 species Over 150 species Males- 2 Males- 2
millimetersmillimeters Females- 3-5 Females- 3-5
millimetersmillimeters Diet of algaeDiet of algae
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Background- Artificial Background- Artificial Sugar (Aspartame)Sugar (Aspartame) Aspartic AcidAspartic Acid 40%40% an excitotoxinan excitotoxin Can pass through the blood brain Can pass through the blood brain
barrierbarrier
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Background- Background- AspartameAspartame PhenylalaninePhenylalanine 50%50% 4% is in protein we eat4% is in protein we eat
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Background- Background- AspartameAspartame Methyl Ester Methyl Ester 10%10% Wood AlcoholWood Alcohol Breaks down into formaldehydeBreaks down into formaldehyde
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Background- Raw Background- Raw SugarSugar Not processed Not processed From crushed sugar canes From crushed sugar canes Thin coating of molassesThin coating of molasses
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HypothesisHypothesis
If the daphnia is exposed to If the daphnia is exposed to artificial sugar containing artificial sugar containing aspartame the daphnia’s aspartame the daphnia’s heartbeat will be higher than it heartbeat will be higher than it would be with raw sugar.would be with raw sugar.
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VariablesVariables
Dependant Variable- Daphnia’s Dependant Variable- Daphnia’s heartbeatheartbeat
Independent Variable- Artificial Independent Variable- Artificial sugar and Raw sugarsugar and Raw sugar
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ControlControl
Control- Daphnia’s heartbeats Control- Daphnia’s heartbeats with waterwith water
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ConstantsConstants
Constants- The daphnia, the Constants- The daphnia, the amount of raw sugar used, the amount of raw sugar used, the amount of artificial sugar used, amount of artificial sugar used, and the time each daphnia was and the time each daphnia was watched.watched.
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MaterialsMaterials
2 Cultures of 2 Cultures of Daphnia MagnaDaphnia Magna
1 Teaspoon1 Teaspoon 1 Microscope 1 Microscope 1 Slide1 Slide Artificial Sugar Artificial Sugar
““EqualEqual”” Raw Sugar Raw Sugar “Sugar “Sugar
in the Raw”in the Raw”
3 Eyedroppers3 Eyedroppers 2 Cups of water2 Cups of water 1 Watch1 Watch
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ProcedureProcedure
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ResultsResults
Daphnia's Heartbeats per Minute
0
100
200
300
400
500
600
1 2 3 4 5
Minutes
Dap
hn
ia H
eart
bea
ts
Water
Equal
Sugar in the Raw
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ResultsResults
Daphnia's Average Heartbeat
Minutes 1 2 3 4 5
Water 218 245 217 267 270
Artificial Sugar 312 502 299 306 304
Raw Sugar 290 293 301 296 298
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ConclusionConclusion
To see how the daphnia’s To see how the daphnia’s heartbeat is affected by the heartbeat is affected by the artificial sugar and the raw sugar.artificial sugar and the raw sugar.
The Hypothesis was acceptedThe Hypothesis was accepted Possible because of the Possible because of the
excitotoxinexcitotoxin Aspartame affects the brain more Aspartame affects the brain more
than the heart.than the heart.
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Used in Real LifeUsed in Real Life
To learn more about what we eat.To learn more about what we eat.
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RecommendationsRecommendations
Learn more about how it affects Learn more about how it affects the heartthe heart
Make the project last longerMake the project last longer
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AcknowledgeAcknowledge
My parentsMy parents J.T. and CoriJ.T. and Cori Mrs. PeffleyMrs. Peffley