th2a - genes &...
TRANSCRIPT
cgtggaaaaataagt----ttgtccactggaacgtctcaaaacagttccaactg
xxxx xx xxxx xxxxxxxxxxxxxxxxxxxxxxxx
------ggctcaagtaacctttcccacaccaacgtctcaaaacagttccaactg
ctgagaaaacacac-cg-cttgaaggccaagaagagaggtatttatag-cgtgg
x xxxxxxxxxxxx xx xxxxx x xxxxx x xxxxxxxxxxx xxxxx
ctgagaaaacacacacgacttgaggttg-agaagcggggtatttataggcgtgg
gccttggatgagctatgagcacgcgaaagatgattggacaataaatgttccttg
x xxxxxxxxxxxxxx x xxx x xx xxxxxxxxxx xxx xx x xx
acattggatgacgtatgggtacatgcaatctgattggacagtaagtgctgggtg
tatgcaaataaggggaattcaagtggtgctttctgattggttaattcatcacca
xxxxxxxxxxxxxxxxxxxxxx xxx xxxxx xxxxxxxxxx xxxxx xx
catgcaaataaggggaattcaagctgtgatttctaattggttaatccatcatca
atcagccaatcaggtggtgagtgtaatttgccattagactataaataggcgtgg
xxxxxxxxxxxxxxxx xxxx xxx xxxxxxxxxxxxxxxxxxxxxxxxxx
atcagccaatcaggtgttgagcataagctgccattagactataaataggcgtgg
acgcacta-cctccgct-acggctttatggcttttgaagaatc
x xxxx xxx xx xxxxxx x xxxxxxxxxxxxx
tcatgggggcctc-gcttaccgctttaagacttttgaagaatc
Th2a
Th2b
Ra
tM
ou
se
TATA
Hexamer CCAAT
Octamer CCAAT
CCAAT TATAOctamer
Approximate TSS positions
BamHINeo
GAPHHHHHHGAAGGDYKDDDDKSAAGGYPYDVPDYA
8.8 kb
BamHI
LoxP
TAG
Th2b
LoxP
His Flag Ha
Th2a //
BamHI
8.8 kb
BamHITAG
X CMV - Cre
Th2bTh2aTh2a //
Th2bNeo/Neo
Th2b +/tag
A
B
Montellier et al. Figure S1
TH2B-less
TH2B-tag
PCR
Th2bNeo
Wild type
1 2 3Southern
12.7
8.8
BamHI digest
C
Th2bTh2a
BamHI
BamHI
BamHI BamHI
12.7 kb// Th2b+/+
TH2B
3’ probe
3’ probe
3’ probe
3’ probe
1 2 3
PCR primers
Mass spectrometry analysis of histone variants
Montellier et al. Figure S2 (related to Figure 4A and B)
IPI00228616
H1
IPI00223713
H1
IPI00331597
H1
IPI00223714
H1
IPI00319556
H1
IPI00467914
H1
IPI00230133
H1
IPI00331734
H2A
IPI00555055
H2A
IPI00623951
H2A
IPI00230264
H2A
IPI00221463
H2A
IPI00229542
H2A
H2A
IPI00378480
IPI00874654
H2B
IPI00330000
H2B
IPI00272033
H2B
IPI00111957
H2B
IPI00114642
H2B
IPI00282269
H2B
IPI00227930
H2B
IPI00554853
H2B
IPI00648991
H2B
IPI00459318
H2B
IPI00785343
H3
IPI00277753
H3
IPI00230730
H3
IPI00407339
H4
Montellier et al. Figure S3
+ -H2AZ + -
Spc R-Spt
Expression profile of H2AZ+
versus H2AZ- genes
Nor
mal
ized
expr
essi
on v
alue
s
p<0.0001* p=0.0004*
B TH2BGFP
tagTH2BGFP
A (related to Figure 4 D to F)
Montellier et al. Figure S4 (related to Figure 5B)
Th2
b+/
+Th
2b
+/t
ag
TH2B TP2 TH2B Prm1
Co-detection of TH2B and TP2 Co-detection of TH2B and Prm1
11500 12000 12500 13000 13500 14000 14500
Mass (Da)
0
10
20
30
40
50
60
70
80
90
100
0
10
20
30
40
50
60
70
80
90
100
Rela
tive
Ab
un
da
nc
e
11331.39
14208.75
13860.44
Th2b +/+
TH2B -less
H4
+1Ac
13916.50
tH2B
H2B type 1-H
and/or 1-K and/or 2-B
13700 13800 13900 14000 14100 14200 14300 14400 14500
Mass (Da)
0
10
20
30
40
50
60
70
80
90
100
0
10
20
30
40
50
60
70
80
90
100
13860.44
H2B type 1-F/J/L
and/or 1-M
TH2B
14208.75
H2A
Type 2-A
13916.50
H2B type 1-P (x7.3)
13876.60
H2B type 1-B (x4.0)
H2B
type 1-C/E/G(x2.4)
H2B
type 1-H
and/or 1-K
and/or 2-B(x2.6)
H2B type 1-F/J/L or 1-M (x2.8)
H2A type 2-A (x1.1)
Montellier et al. Figure S5A (related to Figure 6B)
-0.20
0.00
0.20
0.40
0.60
0.80
1.00
1.20
1.40
1.60
tH2B H2A type 2-A H2B type 1-P Histone H2B type 1-B Histone H2B type 1-F/J/L and/or 1-M
Histone H2B type 1-H or 1-K and/or 2-B
Histone H2B type 1-C/E/G
H2
B/H
4 r
atio
tH2B +/+
tH2B -/-
x1.1
x7.3 x4.0
x2.8
x2.6 x2.4
Th2b +/+
TH2B - less
Montellier et al. Figure S5B (related to Figure 6B)
1min 3min 5min MNase digestion
Th2
b+/
+
Nucleosomes
Sub-nucleosomal particles
TH2
B-l
ess
Th2
b+/
+
TH2
B-l
ess
Th2
b+/
+
TH2
B-l
ess
Figure S5C(related to Figure 6)
MS/MS spectra of the modified peptides in histones
Montellier et al. Figure S5D (related to Figure 6C)
Monoisotopic mass of neutral peptide Mr(calc): 1496.84 Variable modifications: N-term : Propionyl:13C(3) (N-term) K2 : Acetyl (K) K5 : Acetyl (K) K9 : Acetyl (K) K13 : Acetyl (K) Ions Score: 91 Expect: 4.8e-008
MS/MS Fragmentation of GKGGKGLGKGGAKRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 17530: 1496.836954 from(749.425753,2+)
Pr-GKacGGKacGLGKacGGAKacR H4K5ac, K8ac, K12ac; K16ac
Monoisotopic mass of neutral peptide Mr(calc): 982.56 Variable modifications: N-term : Propionyl (N-term) K4 : Acetyl (K) K8 : Acetyl (K) Ions Score: 40 Expect: 0.0023
MS/MS Fragmentation of GLGKGGAKRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 6489: 982.553324 from(492.283938,2+)
Pr-GLGKacGGAKacR H4K12ac, K16ac
Monoisotopic mass of neutral peptide Mr(calc): 1513.87 Variable modifications: N-term : Propionyl:13C(3) (N-term) K2 : Propionyl:13C(3) (K) K5 : Acetyl (K) K9 : Acetyl (K) K13 : Acetyl (K) Ions Score: 70 Expect: 6.6e-006
MS/MS Fragmentation of GKGGKGLGKGGAKRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 27868: 1513.863074 from(757.938813,2+)
Pr-GKprGGKacGLGKacGGAKacR H4K8ac, K12ac, K16ac
Monoisotopic mass of neutral peptide Mr(calc): 1450.81 Variable modifications: N-term : Propionyl (N-term) K8 : Propionyl (K) R12 : Methyl (R) Ions Score: 71 Expect: 2.9e-006
MS/MS Fragmentation of DNIQGITKPAIRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 23701: 1450.809604 from(726.412078,2+)
Pr-DNIQGITKprPAIRme H4R35me
MS/MS Fragmentation of ISGLIYEETRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 10921: 1249.653234 from(625.833893,2+)
Monoisotopic mass of neutral peptide Mr(calc): 1249.66 Variable modifications: N-term : Propionyl (N-term) R10 : Methyl (R) Ions Score: 57 Expect: 4.8e-005
Pr-ISGLIYEETRme H4R55me
MS/MS Fragmentation of GVLKVFLENVIRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 15906: 1512.899574 from(757.457063,2+)
Monoisotopic mass of neutral peptide Mr(calc): 1512.90 Variable modifications: N-term : Propionyl:13C(3) (N-term) K4 : Crotonoyl (K) Ions Score: 73 Expect: 9.3e-007
Pr-GVLKCrVFLENVIR H4K59cr
Monoisotopic mass of neutral peptide Mr(calc): 1058.61 Variable modifications: N-term : Propionyl (N-term) R8 : Methyl (R) Ions Score: 49 Expect: 0.00025
MS/MS Fragmentation of VFLENVIRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 13932: 1058.610514 from(530.312533,2+)
Pr-VFLENVIRme H4R67me
Monoisotopic mass of neutral peptide Mr(calc): 1413.69 Variable modifications: N-term : Propionyl (N-term) K10 : Crotonoyl (K) Ions Score: 41 Expect: 0.002
MS/MS Fragmentation of DAVTYTEHAKRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 26245: 1413.683874 from(707.849213,2+)
Pr-DAVTYTEHAKcrR H4K77cr
Monoisotopic mass of neutral peptide Mr(calc): 1376.70 Variable modifications: N-term : Propionyl:13C(3) (N-term) K10 : Dimethyl (K) Ions Score: 57 Expect: 5.9e-005
MS/MS Fragmentation of DAVTYTEHAKRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 13738: 1376.664954 from(689.339753,2+)
Pr-DAVTYTEHAKme2R H4K77me2
Monoisotopic mass of neutral peptide Mr(calc): 1753.97 Variable modifications: N-term : Propionyl:13C(3) (N-term) K1 : Propionyl:13C(3) (K) K13 : Acetyl (K) Ions Score: 45 Expect: 0.00098
MS/MS Fragmentation of KTVTAMDVVYALKRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 23128: 1754.007234 from(878.010893,2+)
Pr-KprTVTAMDVVYALKacR H4K79ac
Monoisotopic mass of neutral peptide Mr(calc): 1060.60 Variable modifications: N-term : Propionyl:13C(3) (N-term) K1 : Propionyl:13C(3) (K) K6 : Acetyl (K) Ions Score: 53 Expect: 0.00013
MS/MS Fragmentation of KSTGGKAPRFound in gi|6680161, histone H3.3 [Mus musculus]
Match to Query 8527: 1060.595124 from(531.304838,2+)
Pr-KprSTGGKacAPR H3K14ac
Monoisotopic mass of neutral peptide Mr(calc): 1125.65 Variable modifications: N-term : Propionyl (N-term) K1 : Acetyl (K) K6 : Acetyl (K) Ions Score: 46 Expect: 0.00089
MS/MS Fragmentation of KQLATKAARFound in gi|6680161, histone H3.3 [Mus musculus]
Match to Query 14486: 1125.647494 from(563.831023,2+)
Pr-KacQLATKacAAR H3K18ac, K23ac
Pr-KprQLATKacAAR H3K23ac
MS/MS Fragmentation of KQLATKAARFound in gi|6680161, histone H3.3 [Mus musculus]
Match to Query 10716: 1145.683264 from(573.848908,2+)
Monoisotopic mass of neutral peptide Mr(calc): 1145.69 Variable modifications: N-term : Propionyl:13C(3) (N-term) K1 : Propionyl:13C(3) (K) K6 : Acetyl (K) Ions Score: 74 Expect: 5.5e-007
Monoisotopic mass of neutral peptide Mr(calc): 1165.68 Variable modifications: N-term : Propionyl (N-term) K1 : Propionyl (K) K6 : Crotonoyl (K)Ions Score: 48
Expect: 0.00041
MS/MS Fragmentation of KQLATKAARFound in gi|6680161, histone H3.3 [Mus musculus]
Match to Query 20231: 1165.678994 from(583.846773,2+)
Pr-KprQLATKcrAAR H3K23cr
Monoisotopic mass of neutral peptide Mr(calc): 1070.60 Variable modifications: N-term : Propionyl (N-term) K1 : Dimethyl (K) K10 : Propionyl (K) Ions Score: 46 Expect: 0.0005
MS/MS Fragmentation of KSAPSTGGVKFound in gi|6680161, histone H3.3 [Mus musculus]
Match to Query 12259: 1070.593544 from(536.304048,2+)
Pr-Kme2SAPSTGGVKpr H3K27me2
Monoisotopic mass of neutral peptide Mr(calc): 1090.60 Variable modifications: N-term : Propionyl:13C(3) (N-term) K1 : Acetyl (K) K10 : Propionyl:13C(3) (K)Ions Score: 46 Expect: 0.00025
MS/MS Fragmentation of KSAPSTGGVKFound in gi|6680161, histone H3.3 [Mus musculus]
Match to Query 13085: 1090.628694 from(546.321623,2+)
Pr-KacSAPSTGGVKpr H3K27ac
Monoisotopic mass of neutral peptide Mr(calc): 1507.84 Variable modifications: N-term : Propionyl (N-term) K6 : Crotonoyl (K) Ions Score: 49 Expect: 0.00035
MS/MS Fragmentation of VTIMPKDIQLARFound in gi|6680161, histone H3.3 [Mus musculus]
Match to Query 24307: 1507.840244 from(754.927398,2+)
Pr-VTIMPKcrDIQLAR H3K122cr
Monoisotopic mass of neutral peptide Mr(calc): 1661.90 Variable modifications: N-term : Propionyl (N-term) K3 : Acetyl (K) K14 : Propionyl (K) Ions Score: 59 Expect: 3.9e-005
MS/MS Fragmentation of VLKQVHPDTGISSKFound in gi|30061387, histone H2B type 1-H [Mus musculus]
Match to Query 29675: 1661.934114 from(831.974333,2+)
Pr-R-VLKacQVHPDTGISSKpr H2BK46ac
Monoisotopic mass of neutral peptide Mr(calc): 1812.85 Variable modifications: N-term : Propionyl (N-term) R15 : Methyl (R)Ions Score: 72
Expect: 1.7e-006
MS/MS Fragmentation of AMGIMNSFVNDIFERFound in gi|68226433, histone H2B type 2-B [Mus musculus]
Match to Query 19777: 1812.850134 from(907.432343,2+)
Pr-AMGIMNSFVNDIFERme H2BR72me
Monoisotopic mass of neutral peptide Mr(calc): 1916.06 Variable modifications: N-term : Propionyl (N-term) K9 : Acetyl (K) K17 : Propionyl (K) Ions Score: 58 Expect: 9.3e-005
MS/MS Fragmentation of LLLPGELAKHAVSEGTKFound in gi|68226433, histone H2B type 2-B [Mus musculus]
Match to Query 21276: 1916.095494 from(959.055023,2+)
Pr-LLLPGELAKacHAVSEGTKpr H2BK108ac
Monoisotopic mass of neutral peptide Mr(calc): 1889.06 Variable modifications: N-term : Propionyl:13C(3) (N-term) K9 : Crotonoyl (K) Ions Score: 73 Expect: 2.5e-006
MS/MS Fragmentation of LLLPGELAKHAVSEGTKFound in gi|30089704, histone H2B type 1-K [Mus musculus]
Match to Query 22135: 1889.056794 from(945.535673,2+)
Pr-LLLPGELAKcrHAVSEGTK H2B108cr
Monoisotopic mass of neutral peptide Mr(calc): 1406.74 Variable modifications: N-term : Propionyl (N-term) K8 : Crotonoyl (K) K12 : Propionyl (K) Ions Score: 46 Expect: 0.00092
MS/MS Fragmentation of HAVSEGTKAVTKFound in gi|30061387, histone H2B type 1-H [Mus musculus]
Match to Query 26107: 1406.736974 from(704.375763,2+)
Pr-HAVSEGTKcrAVTKpr H2BK116cr
Monoisotopic mass of neutral peptide Mr(calc): 1386.74 Variable modifications: N-term : Propionyl:13C(3) (N-term) K8 : Acetyl (K) K12 : Propionyl:13C(3) (K) Ions Score: 79 Expect: 3e-007
MS/MS Fragmentation of HAVSEGTKAVTKFound in gi|13386452, histone H2B type 3-A [Mus musculus]
Match to Query 22240: 1386.776764 from(694.395658,2+)
Pr-HAVSEGTKacAVTKpr H2BK116ac
Monoisotopic mass of neutral peptide Mr(calc): 919.56 Variable modifications: N-term : Propionyl (N-term) R7 : Methyl (R) Ions Score: 38 Expect: 0.0008
MS/MS Fragmentation of HLQLAIRFound in gi|817939, histone H2A [Mus musculus domesticus]
Match to Query 5038: 919.556554 from(460.785553,2+)
Pr-HLQLAIRme H2AR96me
Monoisotopic mass of neutral peptide Mr(calc): 1300.68 Variable modifications: N-term : Propionyl (N-term) K1 : Propionyl (K) K7 : Crotonoyl (K) K9 : Propionyl (K) Ions Score: 60 Expect: 1.4e-005
MS/MS Fragmentation of KTESHHKAKFound in gi|817939, histone H2A [Mus musculus domesticus]
Match to Query 23609: 1300.675324 from(651.344938,2+)
Pr-KprTESHHKcrAKpr H2AK133cr
Monoisotopic mass of neutral peptide Mr(calc): 1496.84 Variable modifications: N-term : Propionyl:13C(3) (N-term) K2 : Acetyl (K) K5 : Acetyl (K) K9 : Acetyl (K) K13 : Acetyl (K) Ions Score: 91 Expect: 4.8e-008
MS/MS Fragmentation of GKGGKGLGKGGAKRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 17530: 1496.836954 from(749.425753,2+)
Pr-GKacGGKacGLGKacGGAKacR H4K5ac, K8ac, K12ac; K16ac
Monoisotopic mass of neutral peptide Mr(calc): 982.56 Variable modifications: N-term : Propionyl (N-term) K4 : Acetyl (K) K8 : Acetyl (K) Ions Score: 40 Expect: 0.0023
MS/MS Fragmentation of GLGKGGAKRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 6489: 982.553324 from(492.283938,2+)
Pr-GLGKacGGAKacR H4K12ac, K16ac
Monoisotopic mass of neutral peptide Mr(calc): 1513.87 Variable modifications: N-term : Propionyl:13C(3) (N-term) K2 : Propionyl:13C(3) (K) K5 : Acetyl (K) K9 : Acetyl (K) K13 : Acetyl (K) Ions Score: 70 Expect: 6.6e-006
MS/MS Fragmentation of GKGGKGLGKGGAKRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 27868: 1513.863074 from(757.938813,2+)
Pr-GKprGGKacGLGKacGGAKacR H4K8ac, K12ac, K16ac
Monoisotopic mass of neutral peptide Mr(calc): 1450.81 Variable modifications: N-term : Propionyl (N-term) K8 : Propionyl (K) R12 : Methyl (R) Ions Score: 71 Expect: 2.9e-006
MS/MS Fragmentation of DNIQGITKPAIRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 23701: 1450.809604 from(726.412078,2+)
Pr-DNIQGITKprPAIRme H4R35me
MS/MS Fragmentation of ISGLIYEETRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 10921: 1249.653234 from(625.833893,2+)
Monoisotopic mass of neutral peptide Mr(calc): 1249.66 Variable modifications: N-term : Propionyl (N-term) R10 : Methyl (R) Ions Score: 57 Expect: 4.8e-005
Pr-ISGLIYEETRme H4R55me
MS/MS Fragmentation of GVLKVFLENVIRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 15906: 1512.899574 from(757.457063,2+)
Monoisotopic mass of neutral peptide Mr(calc): 1512.90 Variable modifications: N-term : Propionyl:13C(3) (N-term) K4 : Crotonoyl (K) Ions Score: 73 Expect: 9.3e-007
Pr-GVLKCrVFLENVIR H4K59cr
Monoisotopic mass of neutral peptide Mr(calc): 1058.61 Variable modifications: N-term : Propionyl (N-term) R8 : Methyl (R) Ions Score: 49 Expect: 0.00025
MS/MS Fragmentation of VFLENVIRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 13932: 1058.610514 from(530.312533,2+)
Pr-VFLENVIRme H4R67me
Monoisotopic mass of neutral peptide Mr(calc): 1413.69 Variable modifications: N-term : Propionyl (N-term) K10 : Crotonoyl (K) Ions Score: 41 Expect: 0.002
MS/MS Fragmentation of DAVTYTEHAKRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 26245: 1413.683874 from(707.849213,2+)
Pr-DAVTYTEHAKcrR H4K77cr
Monoisotopic mass of neutral peptide Mr(calc): 1376.70 Variable modifications: N-term : Propionyl:13C(3) (N-term) K10 : Dimethyl (K) Ions Score: 57 Expect: 5.9e-005
MS/MS Fragmentation of DAVTYTEHAKRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 13738: 1376.664954 from(689.339753,2+)
Pr-DAVTYTEHAKme2R H4K77me2
Monoisotopic mass of neutral peptide Mr(calc): 1753.97 Variable modifications: N-term : Propionyl:13C(3) (N-term) K1 : Propionyl:13C(3) (K) K13 : Acetyl (K) Ions Score: 45 Expect: 0.00098
MS/MS Fragmentation of KTVTAMDVVYALKRFound in gi|30061349, histone H4 [Mus musculus]
Match to Query 23128: 1754.007234 from(878.010893,2+)
Pr-KprTVTAMDVVYALKacR H4K79ac
Monoisotopic mass of neutral peptide Mr(calc): 1060.60 Variable modifications: N-term : Propionyl:13C(3) (N-term) K1 : Propionyl:13C(3) (K) K6 : Acetyl (K) Ions Score: 53 Expect: 0.00013
MS/MS Fragmentation of KSTGGKAPRFound in gi|6680161, histone H3.3 [Mus musculus]
Match to Query 8527: 1060.595124 from(531.304838,2+)
Pr-KprSTGGKacAPR H3K14ac
Monoisotopic mass of neutral peptide Mr(calc): 1125.65 Variable modifications: N-term : Propionyl (N-term) K1 : Acetyl (K) K6 : Acetyl (K) Ions Score: 46 Expect: 0.00089
MS/MS Fragmentation of KQLATKAARFound in gi|6680161, histone H3.3 [Mus musculus]
Match to Query 14486: 1125.647494 from(563.831023,2+)
Pr-KacQLATKacAAR H3K18ac, K23ac
Pr-KprQLATKacAAR H3K23ac
MS/MS Fragmentation of KQLATKAARFound in gi|6680161, histone H3.3 [Mus musculus]
Match to Query 10716: 1145.683264 from(573.848908,2+)
Monoisotopic mass of neutral peptide Mr(calc): 1145.69 Variable modifications: N-term : Propionyl:13C(3) (N-term) K1 : Propionyl:13C(3) (K) K6 : Acetyl (K) Ions Score: 74 Expect: 5.5e-007
Monoisotopic mass of neutral peptide Mr(calc): 1165.68 Variable modifications: N-term : Propionyl (N-term) K1 : Propionyl (K) K6 : Crotonoyl (K)Ions Score: 48
Expect: 0.00041
MS/MS Fragmentation of KQLATKAARFound in gi|6680161, histone H3.3 [Mus musculus]
Match to Query 20231: 1165.678994 from(583.846773,2+)
Pr-KprQLATKcrAAR H3K23cr
Monoisotopic mass of neutral peptide Mr(calc): 1070.60 Variable modifications: N-term : Propionyl (N-term) K1 : Dimethyl (K) K10 : Propionyl (K) Ions Score: 46 Expect: 0.0005
MS/MS Fragmentation of KSAPSTGGVKFound in gi|6680161, histone H3.3 [Mus musculus]
Match to Query 12259: 1070.593544 from(536.304048,2+)
Pr-Kme2SAPSTGGVKpr H3K27me2
Monoisotopic mass of neutral peptide Mr(calc): 1090.60 Variable modifications: N-term : Propionyl:13C(3) (N-term) K1 : Acetyl (K) K10 : Propionyl:13C(3) (K)Ions Score: 46 Expect: 0.00025
MS/MS Fragmentation of KSAPSTGGVKFound in gi|6680161, histone H3.3 [Mus musculus]
Match to Query 13085: 1090.628694 from(546.321623,2+)
Pr-KacSAPSTGGVKpr H3K27ac
Monoisotopic mass of neutral peptide Mr(calc): 1507.84 Variable modifications: N-term : Propionyl (N-term) K6 : Crotonoyl (K) Ions Score: 49 Expect: 0.00035
MS/MS Fragmentation of VTIMPKDIQLARFound in gi|6680161, histone H3.3 [Mus musculus]
Match to Query 24307: 1507.840244 from(754.927398,2+)
Pr-VTIMPKcrDIQLAR H3K122cr
Monoisotopic mass of neutral peptide Mr(calc): 1661.90 Variable modifications: N-term : Propionyl (N-term) K3 : Acetyl (K) K14 : Propionyl (K) Ions Score: 59 Expect: 3.9e-005
MS/MS Fragmentation of VLKQVHPDTGISSKFound in gi|30061387, histone H2B type 1-H [Mus musculus]
Match to Query 29675: 1661.934114 from(831.974333,2+)
Pr-R-VLKacQVHPDTGISSKpr H2BK46ac
Monoisotopic mass of neutral peptide Mr(calc): 1812.85 Variable modifications: N-term : Propionyl (N-term) R15 : Methyl (R)Ions Score: 72
Expect: 1.7e-006
MS/MS Fragmentation of AMGIMNSFVNDIFERFound in gi|68226433, histone H2B type 2-B [Mus musculus]
Match to Query 19777: 1812.850134 from(907.432343,2+)
Pr-AMGIMNSFVNDIFERme H2BR72me
Monoisotopic mass of neutral peptide Mr(calc): 1916.06 Variable modifications: N-term : Propionyl (N-term) K9 : Acetyl (K) K17 : Propionyl (K) Ions Score: 58 Expect: 9.3e-005
MS/MS Fragmentation of LLLPGELAKHAVSEGTKFound in gi|68226433, histone H2B type 2-B [Mus musculus]
Match to Query 21276: 1916.095494 from(959.055023,2+)
Pr-LLLPGELAKacHAVSEGTKpr H2BK108ac
Monoisotopic mass of neutral peptide Mr(calc): 1889.06 Variable modifications: N-term : Propionyl:13C(3) (N-term) K9 : Crotonoyl (K) Ions Score: 73 Expect: 2.5e-006
MS/MS Fragmentation of LLLPGELAKHAVSEGTKFound in gi|30089704, histone H2B type 1-K [Mus musculus]
Match to Query 22135: 1889.056794 from(945.535673,2+)
Pr-LLLPGELAKcrHAVSEGTK H2B108cr
Monoisotopic mass of neutral peptide Mr(calc): 1406.74 Variable modifications: N-term : Propionyl (N-term) K8 : Crotonoyl (K) K12 : Propionyl (K) Ions Score: 46 Expect: 0.00092
MS/MS Fragmentation of HAVSEGTKAVTKFound in gi|30061387, histone H2B type 1-H [Mus musculus]
Match to Query 26107: 1406.736974 from(704.375763,2+)
Pr-HAVSEGTKcrAVTKpr H2BK116cr
Monoisotopic mass of neutral peptide Mr(calc): 1386.74 Variable modifications: N-term : Propionyl:13C(3) (N-term) K8 : Acetyl (K) K12 : Propionyl:13C(3) (K) Ions Score: 79 Expect: 3e-007
MS/MS Fragmentation of HAVSEGTKAVTKFound in gi|13386452, histone H2B type 3-A [Mus musculus]
Match to Query 22240: 1386.776764 from(694.395658,2+)
Pr-HAVSEGTKacAVTKpr H2BK116ac
Monoisotopic mass of neutral peptide Mr(calc): 919.56 Variable modifications: N-term : Propionyl (N-term) R7 : Methyl (R) Ions Score: 38 Expect: 0.0008
MS/MS Fragmentation of HLQLAIRFound in gi|817939, histone H2A [Mus musculus domesticus]
Match to Query 5038: 919.556554 from(460.785553,2+)
Pr-HLQLAIRme H2AR96me
Monoisotopic mass of neutral peptide Mr(calc): 1300.68 Variable modifications: N-term : Propionyl (N-term) K1 : Propionyl (K) K7 : Crotonoyl (K) K9 : Propionyl (K) Ions Score: 60 Expect: 1.4e-005
MS/MS Fragmentation of KTESHHKAKFound in gi|817939, histone H2A [Mus musculus domesticus]
Match to Query 23609: 1300.675324 from(651.344938,2+)
Pr-KprTESHHKcrAKpr H2AK133cr
Montellier et al. Figure S6
A
Male Th2b+/+
XFemale Th2b+/+
Male TH2B-lessX
Female TH2B-less
B
Male Th2b+/+
XFemale Th2b+/+
Male Th2b+/+
XFemale Th2b+/tag
Male Th2b+/tag
XFemale Th2b+/+
H2B
Ha
TH2B
DNA
Montellier et al. Figure S7 (related to Figure 7A)
DNA
DNA
13.5 d. p. c embryos