testing of beverages a testing lab’s perspective
TRANSCRIPT
Testing of beverages a testing lab’s perspective
Challenges and opportunities in testing beverages
What all is to be tested?
Chemical Testing Quality parameters
Safety Parameters
Nutritional Parameters
Biological Testing Indicator and Hygiene Parameters
Pathogens
Viruses and parasites
GMO detection
Meat and Fish Species identification
What to test and methods to follow?
Standard products – easy – do what is in the act and rules
Proprietary/Non standard – not in the Act and Rules – risk based approach
Methods – FSSAI manual?
Standard methods?
In-house validated methods?
Must verify!!!!!!
Must test multiple batches in the beginning!!!!!!
Capabilities requiredMajor tests that need to be conducted by the laboratory
Shelf life estimation Real-time vs Accelerated
What protocol to follow?
Test? Quality? Assay for claim ingredients? Sensory? Safety? Microbiology? Pack integrity? Acceptance criteria? Methods – verified or valiated for
the product
Time points? Real-time?
Accelerated
What multiplier?
WVTR and OTR enough?
What storage temperature? 37, 40, 45, -20, 2 C
Humidity? 75%, 90%, 30% or uncontrolled
Nutritional analysis
Proximate analysis
Capabilities Fat
Protein
Carbohydrate
Energy
Ash
Moisture
Equipment Automated soxhlet
CHNS & Auto Kjeldahl
Bomb calorie meter
Thermo gravimetric analyser
RI HPLC?
GC FID?
Sugar profile
Capabilities Glucose
Fructose
Sucrose
Trehalose
Triose, Tetrose etc
Equipment HPLC – RI
Amino acid profile
Capabilities Protein powders
Nutraceuticals
Free amino acids
Amino acid profile of protein
Equipment HPLC – FLD
HPTLC
Fat and water soluble Vitamins
Capabilities Juices
Fortified foods
Nutraceuticals
All vitamins Water soluble – Vitamin C, B1, B2,
B6, B12
Fat soluble – Vitamin A, E, D, K
Equipment HPLC
LCMSMS
Essential and trace element
Capabilities Analysis of elements from 100% to
ppb levels
Methods for various matrices Fortified foods
Packages foods
Nutraceuticals
RTE etc
Equipment ICPMS
Ion chromatography
Auto titrators
Microwave digestion
Fibre analysis
Capabilities Crude fibre
Dietary fibre
Equipment Automated fibre analyser
HPLC RI Detctor
Herbal ingredients
Capabilities Chromatographic fingerprinting
Marker compound quantification
Poly herbal formulations
Equipment HPTLC
HPLC
GC
GCMSMS
LCMSMS
Fatty acid profile
Capabilities Fats and oils
Nutraceuticals
Packaged foods etc
All fatty acids
Trans fats
Cholesterol
Equipment GC – FID
FTIR
Claims verification
Claims Sugar free High protein Isotonic Low fat Low salt Trans fate free High fibre content Cholesterol free Preservative free Organic
Equipment Automated soxhlet Auto titrator GC Fibertech RI HPLC GC HPLC/LCMSMS LCMSMS, GCMSMS CHNS – dumas method
Food Safety Parameters Comprehensive menu of tests covering all tests under FSSA and more
Pesticide Residue
Capabilities Capability to analyse all
pesticides in FSSA - 149
Detection levels below than current regulations
Various matrices
Equipment GCMSMS
LCMSMS
HPLC
Sample mills
Homogenizers
Sample concentrator and hoods
Heavy metals
Capabilities Ability to analyse all toxic metals
at ultra trace levels
Using standard international methods
Methods suitable for variety of food matrices
Equipment ICPMS
Microwave digestion system
Fume hoods
Clean lab
Ultra pure acids
NOTS – Naturally occurring toxins
Capabilities All NOTS as per FSSA
Safrole
Hypercine
Hydrocyanic acid
Mycotoxins
Equipment HPLC – FLD
HPTLC
GCMSMS
LCMSMS
Ion chromatography
Mycotoxins
Mycotoxins Aflatoxins – B1, B2, G1, G2
Ochratoxin
Patulin
ZON, DON
Equipment HPLC – FLD
LCMSMS
ELISA – for screening
Vet drug residues
Capabilities All drugs and matrices as per FSSA
Nitro furans
Tetracycline
Sulphonamides
Methods validated to global standards
Various matrices – Honey, Fish, Milk, Egg
Deuterated internal standards
Equipment LCMSMS
HPLC
ELISA – for screening
Solid phase extraction (SPE)
Mills
Homogenizers
Nitrogen evaporators
Microbiology
Capabilities Pathogen detection
Hygiene monitoring Swabs
Air sampling
Probiotics
Vitamin assays
Facilities Clean rooms
LAF
Biosafety cabinet
Incubators
Freezers
Autoclaves
Food Additives
Capabilities ID and quality of additives
ID and level of additives in food
Food colour – permitted & levels
Preservatives
Anti oxidants
Thickeners, Modifiers, emulsifiers & more
Equipment HPLC
GC
FTIR
HPTLC
GCMSMS
Ion chromatography
Packing material testing?New regulation
Thank you! E-mail – [email protected]
State of the Art equipment
LCMSMS with UPLC Front end
ICP - MS
HPTLC – for Herbal Products
GC-HS-FID and GC-ECD