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Template for comments - Draft ISPMs for country consultation, 2009

DRAFT: Annex to ISPM No. 27 (DIAGNOSTIC PROTOCOLS FOR REGULATED PESTS)

Thrips palmi

Australia, Burundi, China (+APPPC), Congo, COSAVE, EC, Egypt, Ecuador, Grenada, IASPC+Gabon, Israel, Ivory Coast, Japan, Kenya, Korea, Lebanon+Tunisia, Malaysia, Mexico, Mozambique, Nepal, New Zealand, Nigeria, Peru,

Philippines, Seychelles, South Africa, Swaziland, Trinidad and Tobago, Togo USA, Zambia (compiled by JKI/Plant Health Institute/Steffen)

1. Section 2. Para nber

3. sentence/row/indent, etc.

4. Type of comment (Substantive,Editorial,Translation)

5. Proposed rewording 6. Explanation 7. Country

GENERAL COMMENTS

Australia does not offer any objection to the adoption of the revised diagnostic protocol for Thrips palmi. It thanks the author(s) and the Technical Panel for addressing the majority of the concerns that Australia expressed in relation to earlier drafts.Some comments are offered in good faith that may be considered as editorial in finalising the document for publication.

Australia

COMENTARIOS GENERALES

México reconoce que el proyecto ha sido mejorando de sobremanera y que ayudará a los países a realizar una correcta identificación de la especie Thips palmi., por lo que apoya su adopción por parte de la CIPF.

El Grupo de Expertos que redactó el protocolo tomó en cuenta varios de los comentarios hechos por los países durante el pasado proceso de revisión de normas, lo que mejoró el contenido del mismo.

De igual manera, se reconoce el hecho de que agregaron fotos más verídicas que esquemáticas, lo que da mayor referencia al lector acerca de las características de diagnóstico específicas propias de la plaga.

México





Los comentarios que presenta México al proyecto son cuestiones editoriales y de redacción con la intención de mejorar su entendimiento.

GENERAL COMMENTS

The draft is very technical it will take time with sufficient knowledge and it will involved a lot to enforce the capacity building to meet this requirement..However we understand the responsibilities of the activity but we don’t have ability to do the recommendation. Diagnostic capability is still one of our main weaknesses therefore it application can be difficult at this point of time.

Seychelles

GENERAL COMMENTS

High quality diagnostic images are provided as an Australian publication on the IPP. These images complement the draft diagnostic protocol for T. palmi.

Australia

GENERAL COMMENTS

Substantive It is proposed that the magnification levels that were used in photomicrographs in this document should be added at each photomicrograph for easy reference.

Throughout the document valuable and user-friendly photomicrographs are being used. To further enhance the usefulness of these photomicrographs it is recommended that the magnification levels that were used to identify certain characteristics in this document be indicated.

This will help the user to do microscopic examination of thrips.

South Africa

GENERAL COMMENT Substantive Unit µl should be replaced with µL, throughout the protocol.

For the symbol of the liter, the use of L instead of l is a recommendation of the "International Committee for Weights and Measures". Whichever is used (i.e. µl or µL) this should be used for this unit in all future DPs and agreed as part of the Instructions to Authors.

EC

GENERAL COMMENTS

Substantive Suggest to add identification key for the similar species at the back of the standard.

Easy for use. When identifying the pest, the most difficult thing is differentiate the similar species. If there is a identification key it will be a great help to the person who use the standard.

China





GENERAL COMMENTS

Substantive 1. The annex requires special skills to use and a statement on this effect should be provided in the document2. All titles and subtitles be consistent in formatting

Kenya

GENERAL COMMENTS

Editorial Thrips (T. flavus, T. major Uzel, T. minutissimus L., T. nigropilosus, T. sambuci Heeger, T. tabaci, T. trehernei (T. physapus), T. urticae, T. validus Uzel, T. vulgatissimus Haliday).

All sub-heading should be numbered consistently through out the document.

To add the authors’ names for each species in all the document

To prevent confusion and to make it simple to read the document.

Egypt

GENERAL COMMENTS

Editorial Thrips (T. flavus, T. major Uzel, T. minutissimus L., T. nigropilosus, T. sambuci Heeger, T. tabaci, T. trehernei (T. physapus), T. urticae, T. validus Uzel, T. vulgatissimus Haliday).

All sub-heading should be numbered consistently through out the document.

To add the authors’ names for each species in all the document

To prevent confusion and to make it simple to read the document.

Lebanon -Tunisia

TITLE [1] Editorial Annex 1 to ISPM no. 27 (DIAGNOSTIC PROTOCOLS FOR REGULATED PESTS)

Maybe adding additional annexes in the future for the ISPM

Egypt

TITLE [1] Editorial Annex 1 to ISPM no. 27 (DIAGNOSTIC PROTOCOLS FOR REGULATED PESTS)

Maybe adding additional annexes in the future for the ISPM

Lebanon -Tunisia

TITLE [1] Paragraph 1 Substantive Annex 1 to ISPM No. 27 (DIAGNOSTIC PROTOCOLS FOR REGULATED PESTS)

To be in sequence for recognition and reference when taking into consideration other Annexes that might develop in the near future.

Seychelles

TITLE [2] Editorial Thrips palmi Karny Keeping the right of the author Egypt

TITLE [2] Editorial Thrips palmi Karny Keeping the right of the author Lebanon -TunisiaTABLE DES MATIÈRES

[3] Ligne 2 Rédactionnel Adoption Compréhension du texte BURUNDI

Contents [3] Ligne 2 Rédactionnel Adoption Compréhension du texte R.D. CONGO, TOGO

TABLE DES MATIÈRES

[3] Ligne 2 Traduction Acceptation Adoption Compréhension du texte Côte d’Ivoire

TABLE DES [3] Ligne 15 Rédactionnel Analyse PCR RELP à partir d’une séquence Compréhension du texte BURUNDI





MATIÈRES ITS2 pour neuf espèces

Contents [3] Ligne 15 Rédactionnel Analyse PCR RELP à partir d’une séquence ITS2 pour neuf espèces

Compréhension du texte R.D. CONGO, TOGO

TABLE DES MATIÈRES

[3] Ligne 15 Rédactionnel Analyse PCR RELP à partir d’une séquences ITS2 pour neufs espèces

Compréhension du texte Côte d’Ivoire

Acceptation [4] Ligne 1 Rédactionnel Adoption Compréhension du texte BURUNDI

Endorsement [4] Ligne 1 Rédactionnel Adoption Compréhension du texte R.D. CONGO, TOGO

Acceptation [4] Ligne 1 Traduction Acceptation Adoption Compréhension du texte Côte d’Ivoire1. Pest Information [5]

1. Pest Information [6] sentence1 Substantive Thrips palmi Karny (Thysanoptera:Thripidae) is a polyphagous

It should be in detail Nepal

1. Pest Information [6] 1st sentence Substantive Thrips Palmi Karny is a polyphagous plant pest, especially of species in the Cucurbitaceae and Solanaceae families.

For clarity Grenada

1. Pest Information [6] Sentence 1 Editorial Thrips palmi Karny is a polyphagous plant pest, especially species in the Cucurbitaceae and Solanaceae families.

Clarity Trinidad and Tobago

1. Información sobre la plaga

[6] Traducción Thrips palmi Karny es una plaga polífaga de las plantas, especialmente de especies de Cucurbitáceas y Solanáceas. Parece haberse ser originado rio de en Asia meridional y haberse propagado desde allí durante la segunda parte del siglo XX. Actualmente se encuentra presente en Asia y está muy extendida por todo el Pacífico y el Caribe. Además, se da de encuentra de forma localizada en América del Norte, América Central, América del Sur, África y Oceanía. Para obtener más información general acerca de T. palmi, véanse EPPO/CABI (1997) o Murai (2002); asimismo pueden consultarse las hojas de información sobre plagas, en versión electrónica, de Pests and Diseases Image Library (PaDIL, 2007) y EPPO (EPPO, 2008).

Mejora la redacción del documento Ecuador

1. Pest Information [6] Line 4 Editorial It is now present throughout Asia and is widespread throughout the Pacific and

To avoid cofusion MALAYSIA





Carribean. Otherwise, It occurs locally in North, Central and South America, Africa and Oceania.

1. Pest Information [6] Sentence 3 substantive It has been recorded is now present throughout Asia and is widespread throughout the Pacific and the Caribbean. Otherwise, it has been recorded occurs locally in North, Central and South America, Africa and Oceania

For clarity of the paragraph: Swaziland

1. Pest Information [6] Sentence 3 Substantive It is now present has been recorded throughout Asia and Wide spread throughout the Pacific and the Carribbean: It occurs locally has also been loccally recordéd in North, Central, and South America, Africa and Oceania:

For clarity of the paragraph: Kenya

1. Pest Information [6] Sentence “3 substantive It has been recorded throughout Asia and and recorded locally throughout the Pacific and the Carribbean: it has also been loccally recordéd in North, Central, and South America, Africa and Oceania:

For clarity of the paragraph: IASPC-GABON


For clarity of the paragraph: Mozambique

1. Pest Information [6] Sentence 3 Substantive It has been recorded throughout Asia and wide spread throughout the Pacific and the Carribeans. It has been locally recorded in North, Central and South America, Africa and Oceania

For clarity of the paragraph NIGERIA


For clarity of the paragraph: Zambia

1. Informations sur l'organisme nuisible

[6] Phrase 2

Phrase 3

De fond

Rédactionnel

Il semble qu’il provienne d’Asie du Sud, d'où il se serait propagé vers la fin du vingtième siècle.

Il est également présent localement en

Supprimer car l’origine ne semble pas bien connue de l’auteur (Pas de reference origine)

Compréhension du texte

Côte d’Ivoire





Amérique du Nord, centrale et du Sud, en Afrique et en Océanie.


[6] Phrase 2

Phrase 3

De fond

Rédactionnel Il est présent localement en Amérique du Nord, centrale et du Sud, en Afrique et en Océanie.

L’origine ne semble pas être bien connue de l’auteur (Pas de referencie origine)


BURUNDI


[6] Phrase 2

Phrase 3

De fond

RédactionnelIl est présent localement en Amérique du Nord, centrale et du Sud, en Afrique et en Océanie.

L’origine ne semble pas bien connue de l’auteur (Pas de reference origine)


R.D. CONGO, TOGO

1. Pest Information [6] sentence 4 Editorial Delete reference to Oceania. Oceania is located within the Pacific and is picked up in the previous sentence where the Pacific is referenced.

Australia

1. Pest Information [7] Sentence 3 Editorial Rewrite and insert the list of commodities of which Thrips palmi is a pest in a table format

Listing species in a table format will make it easier to read and search for a specific species when necessary.

South Africa

1. Pest Information [7] Sentence 3 Substantive It is an outdoor pest of, amongst others , Benincasa hispida, Capsicum annum, ....

To indicate that this may not be a comprehensive list and that it is subject to change due to host shifts that may occur.

South Africa

1. Pest Information [7] Last sentence Technical ... as well as on or associated with packing material and in soil.

While thrips would not last long on packing material in isolation – they may be found there immediately after the plant material on which the thrips were had been removed. They may occur in soil in which the thrips had pupated as a much greater risk than packing material isolated from plant material.

Australia

1. Pest Information [7] Last sentence of paragraph

Substantive The tthrips may be carried on plants for planting, cut flowers and, fruits and fruit tissues of host species, as well as on packing material.

Because the eggs and/or larvae of the pest might be inside the fruit tissues.

Egypt

1. Pest Information [7] Proposed new last (former first) sentence of paragraph

Substantive The species causes economic damage to plant crops both as a direct result of its feeding activity and from its ability to vector tospoviruses such as Groundnut bud necrosis virus, Melon yellow spot virus and

In this paragraph we need to know the vector of virus is persistent or not for more elaboration.

Egypt





Watermelon silver mottle virus.1. Pest Information [7] Last sentence of

paragraphSubstantive The thrips may be carried on plants for

planting, cut flowers, fruits and fruit tissues of host species, as well as on packing material.The species causes economic damage to plant crops both as a direct result of its feeding activity and from its ability to vector tospoviruses such as Groundnut bud necrosis virus, Melon yellow spot virus and Watermelon silver mottle virus.

Because the eggs and/or larvae of the pest might be inside the fruit tissues.

In this paragraph we need to know the vector of virus is persistent or not for more elaboration.

Lebanon -Tunisia


[7] Sustancial La especie plaga causa daños económicos a los cultivos de plantas tanto como por el resultado directo de su actividad de alimentación como y por su capacidad para ser vector de tospovirus como: Groundnut bud necrosis virus, Melon yellow spot virus y Watermelon silver mottle virus. Es sumamente polífaga y se ha registrado en más de 36 familias de plantas. Es una plaga de exteriores que afecta a Benincasa hispida, Capsicum annuum, Citrullus lanatus, Cucumis melo, Cucumis sativus, Cucurbita spp., Glycine max, Gossypium spp., Helianthus annuus, Nicotiana tabacum, Phaseolus vulgaris, Pisum sativum, Sesamum indicum, Solanum melongena, Solanum tuberosum y Vigna unguiculata. En invernaderos, entre los hospedantes de importancia económica se encuentran Capsicum annuum, Chrysanthemum spp., Cucumis sativus, Cyclamen spp., Ficus spp., las Orquidáceas y Solanum melongena. Los trips pueden transportarse en plantas para plantar, flores cortadas y frutas de especies hospedantes, así como en el los embalaje.

Aclara en texto Ecuador


[7] Oración 1 Editorial La especie causa daños económicos a los cultivos de plantas tanto como resultado directo de su actividad de alimentación así como por su capacidad para ser vector de

Redacción más clara México





tospovirus como Groundnut bud necrosis virus, Melon yellow spot virus y Watermelon silver mottle virus

1. Pest Information [8] Sentence 1 Editorial Thrips T . palmi No need to write full name in each writing Nepal


[8] Phrase 1 De fond Thrips palmi est presque intégralement de couleur jaune (Figures 1 à 3) et son identification est rendue difficile tant par ses faibles dimensions petite taille (1,0-1,3 mm) que par sa grande similitude avec d'autres espèces de Thrips de couleur jaune ou principalement à prédominance jaune.


Compréhension

Côte d’Ivoire


[8] 1.Phrase 1

2. Ligne 3

De fond Thrips palmi est presque intégralement de couleur jaune (Figures 1 à 3) et son identification est rendue difficile tant par ses petites tailles (1,0-1,3 mm) que par sa grande similitude avec d'autres espèces de Thrips de couleur jaune ou à prédominance jaune.

Compréhension du texte BURUNDI


[8] Phrase 1 De fond Thrips palmi est presque intégralement de couleur jaune (Figures 1 à 3) et son identification est rendue difficile tant par sa petite taille (1,0-1,3 mm) que par sa grande similitude avec d'autres espèces de Thrips de couleur jaune ou à prédominance jaune.

Ccompréhension du texte R.D. CONGO, TOGO


[8] Editorial Thrips palmi es casi enteramente de color amarillo (figuras 1-3) y su identificación resulta difícil debido tanto a su pequeño tamaño (1,0-1,3 mm) como y a su gran similitud con algunas otras especies amarillas o predominantemente amarillas de Thrips.

Da claridad el texto Ecuador

Figure 1: [9] Addition to figure and new figure

Substantive Suggest a scale bar be added.

suggest an additional photograph showing the symptoms of pest effects.

To clarify the size of the insect pictured

To aid diagnosis of the pest.

Korea

Figure 1: [9] Addition to figure and new figure

Substantive Suggest a scale bar be added.

Would suggest an additional photograph showing the symptoms of pest effects.

To clarify the size of the insect pictured

To aid diagnosis of the pest.

Philippines





Figure 2: [10] Ligne 2

Ligne 2

de fond - préciser le grossissement de la figure 2

- Présenter à la même échelle que figure 3

Pour avoir une meilleure idée de la taille de l”insectePour une meilleure comparaison des figures présentées

Côte d’Ivoire

Figure 2: [10] Fig. 2 de fond préciser le grossissement de la figure 2

Présenter à la même échelle que figure 3

Pour avoir une meilleure idée de la taille de l”insecte

Pour une meilleure comparaison des figures présentées

BURUNDI

Figure 2: [10] de fond préciser le grossissement de la figure 2


Pour avoir une meilleure idée de la taille de l”insecte


R.D. CONGO, TOGO

Figure 3: [11] Ligne 2

Ligne 2

de fond préciser le grossissement de la figure 3

Présenter à la même échelle que la figure 2

Pour avoir une meilleure idée de la taille de l’insectePour une meilleure comparaison des figures présentées

Côte d’Ivoire

Figure 3: [11] Fig. 3 de fond préciser le grossissement de la figure 3


Pour avoir une meilleure idée de la taille de l’insecte


BURUNDI

Figure 3: [11] de fond préciser le grossissement de la figure 3


Pour avoir une meilleure idée de la taille de l’insecte


R.D. CONGO, TOGO

2. Información taxonómica

[12] Frase 5 Sustancial Chloeothrips (Mictothrips) aureus Descrita inicialmente como Mictothrips aureus, la especie fue luego reubicada bajo el género Chloethrips para ser finalmente

Perú





reasignada como sinónimo de Thrips palmi. La nomenclatura así definida permite un manejo bibliográfico más certero. Es presentado de está manera por Mound L.en el Checklist de Thysanoptera del Mundo publicado por el CSIRO (1994-2009)

2. Données taxonomiques

[13] Ligne 1Ligne 2Ligne 7Ligne 8

Rédactionnel Name: NomSynonyms SynonymeTaxonomic position Position TaxonomiqueCommon name: Nom commun

Traduction de l’Anglais en Francais Côte d’Ivoire

2. Données taxonomiques

[13] Tirets 1, 2, 3 et 4

Rédactionnel Nom Synonyme Position Taxonomique Nom commun

Traduction BURUNDI

2. Taxonomic Information

[13] Rédactionnel Nom Synonyme Position Taxonomique Nom commun

Traduction R.D. CONGO, TOGO

3. Detection [14] Heading Editorial Detection and Specimen Collection The section covers symptoms and where to look for the Thrips as well as collecting good specimens for diagnosis

Australia

3. Détection [15] Ligne 1 De fond T. palmi peut être localisé un fonctionne des différent stage des développement : est présent dans différentes situations selon son stade de développement:


3. Détection [15] Ligne 1 De fond T. palmi peut être localisé en fonction de ses différent stades de développement :

La compréhension du texte BURUNDI

3. Detection [15] De fond T. palmi peut être localisé un fonctionne des différent stage des développement :

La compréhension du texte R.D. CONGO

3. Detection [15] 1st sentence Editorial T. Palmi may be found in different situations locations according to depending on the life stage present

Clarity Israel





3. Detection [15] Ligne 1 De fond La localisation de T. palmi diffère selon son stade de développement :

La compréhension du texte Togo

3. Detection [16] 1st sentence Editorial On plant material, T.palmi may potentially be found on most above-ground parts of the plant

Clarity Grenada

3. Detection [16] Sentence 1 Editorial On plant material, T. palmi may potentially be found on most aboveground


3. Detection [16] Sentence 1 Editorial .....of the plant; . The parts of of the plant infested can differ accoring to variables such as the host and the characteristics of each seperate T palmi population.

Delete semi-colon and start a new sentence for easier reading/ understanding.

South Africa

3. Detección [16] Redacción En el material vegetal, T. palmi puede encontrarse en la mayoría de las partes de la planta por encima del suelo; las partes de la planta infestadas pueden diferir en función del variables tales como el hospedante y las características de cada población separada de T. palmi

Aclara el texto Ecuador

3. Détection [16] Ligne 2 De fond Les parties infestées de la plante peuvent être différentes peuvent différer selon certaines variables, telles que l'hôte et les caractères propres à chaque population de T. palmi.


3. Détection [16] Ligne 2 De fond Les parties infestées de la plante peuvent être différentes selon certaines variables, telles que l'hôte et les caractères propres à chaque population de T. palmi.


3. Detection [16] De fond Les parties infestées de la plante peuvent être différentes selon certaines variables, telles que l'hôte et les caractères propres à

La compréhension du texte R.D. CONGO, TOGO





chaque population de T. palmi.3. Detección [17] Sustancial Durante el examen visual del material

vegetal para detectar la presencia de T. palmi, se debe prestar atención a cicatrices plateadas, resultantes de alimentación, en la superficie de las hojas de las plantas hospedantes, especialmente paralelas a la nervadura central y las venas. Las plantas que están muy infestadas se caracterizan con frecuencia por la apariencia plateada o bronceada de las hojas, la presencia de hojas y yemas apicales atrofiadas o frutos con cicatrices y deformaciones. La detección puede resultar difícil en las siguientes circunstancias:

– la infestación poblacion en a bajos niveles, que puede producir pocos síntomas o síntomas imposibles de detectar; – la presencia en la planta de huevos dentro del tejido vegetal tan solo (por ejemplo, después de un tratamiento externo que pueda haber eliminado los estados de vida visibles).

Aclara el texto Ecuador

3. Detección [17] Sangría 1Sangría 2

Editorial Durante el examen visual del material vegetal para detectar la presencia de T. palmi, se debe prestar atención a cicatrices plateadas, resultantes de la alimentación, en la superficie de las hojas de las plantas hospedantes, especialmente paralelas a la nervadura central y las venas. Las plantas que están muy infestadas se caracterizan con frecuencia por la apariencia plateada o bronceada de las hojas, la presencia de hojas y yemas apicales atrofiadas o frutos con cicatrices y deformaciones. La detección puede resultar difícil en las siguientes circunstancias:

– la una infestación a bajos niveles, que puede producir pocos síntomas o síntomas imposibles de detectar;

Redacción más clara y para ser equivalente con la versión al inglés.

México





– la presencia en la planta de huevos, dentro del tejido vegetal tan solo (por ejemplo, después de un tratamiento externo que pueda haber eliminado los estados de vida visibles).

Redacción más clara

3. Detección [17] Frase 2 Sustancial Las plantas………………-…………………………….-…….Estados de vida visibles).- la similitud con daños debidos a agentes causales ajenos a la acción de la plaga como enfermedades, otros insectos, actividades culturales, etc. - la acción sinérgica del complejo thrips-fitopatógeno en cuyo caso la superposición de daños no permite una evaluación exclusiva del perjuicio causado por Thrips palmi.

-El daño producido por Thrips palmi, incluso para un especialista medianamente entrenado, puede ser fácilmente confundido con los síntomas de algunas enfermedades, el daño producido por otros insectos o por un mal manejo cultural.- Debido al aparato bucal que poseen, los thrips han desarrollado una relación muy estrecha con tospovirus y otros microorganismos, dispersándolos.

Perú

3. Detection [17] Indent 2 Editorial the presence of the eggs on the plant of the eggs and within the plant tissue only (for example after external treatment which may have removed visible life stages).

For clarity Seychelles

3. Detection [17] 2nd indent substantive Delete ...the presence. “on the plant” of... To improve calrity China

3. Detection [17] Indent 2 editorial the presence on the plant of the eggs within the plant tissue only

For clarity NIGERIA

3. Detection [17] Indent 2 Editorial The presence on the plant of the eggs within the plant tissue only…

Clarity Kenya

3. Detection [17] Indent 2 Editorial The presence of the eggs within the plant tissue only…

clarity IASPC-GABON


clarity Mozambique

3. Detection [17] Indent 2 Editorial the presence on the plant of the eggs within the plant tissue only.

clarity Swaziland


clarity ZAMBIA

3. Detection [17] Phrase 3 Rédactionnel - lors d'une infestation de faible ampleur produisant des symptômes difficiles ou

Clarté Togo





impossibles à déceler;

- lorsque les œufs ne sont présents que

dans le tissu végétal (par exemple après un traitement extérieur susceptible d'avoir éliminé les stades visibles).

3. Detection [18] Sentence 1 Substantive Specimens for morphological examination are best collected in a fluid called AGA, which is a mixture of ……………acetic acid.

For clarity, because AGA is an abbreviation for a mixture of three chemical substances

NIGERIA

3. Detection [18] Sentence 1 substantive Specimens for morphological examination are best collected in a fluid called AGA which is (a a mixture of 10 parts of 60% ethanol with 1 part of glycerine and 1 part of acetic acid)

Clarity and consistence Kenya

3. Detection [18] Sentence 3 substantive AGA written in full as (Alcohol, Glycerine and Acetic acid and put AGA in brackets, which is a mixture of…or may be referred to glossary.

Clarity and consistence IASPC-GABON


Clarity and consistence Mozambique

3. Detection [18] Sentence 1 Substantive ....best collected in a fluid called AGA (Alcohol-Glycerine-Acetic acid ) which is a mixture of 10 parts ...

Indicate the meaning of the abbreviation AGA that is used here for the first time.

South Africa


Clarity and consistence Swaziland


Clarity and consistence ZAMBIA

3. Détection [18] Fond AGA et ADN: à ecrire en toute lettre Clarité BURUNDI





3. Detección [18] Editorial Si se recolectan especímenes para realizar un examen morfológico, conviene conservarlos en un fluido liquido que se llama AGA, el cual es una mezcla de 10 partes de etanol al 60 % con 1 parte de glicerina y 1 parte de ácido acético. Si los especimenes se van a almacenar, deberían transferirse a etanol al 60 % y guardarse en un lugar oscuro, preferiblementeenuncongelador para evitar que pierdan el color. No obstante, varios laboratorios han señalado que el AGA puede tener el un efecto de desnaturalizar el ADN del trips, lo que dificulta la realización posterior de trabajos moleculares. Una alternativa consiste en utilizar etanol al 80-95 % como fluido de conservación, pues en ese caso se pueden utilizar para los estudios moleculares todos los especímenes que no hayan sido montados. En tal caso, sin embargo, los especímenes deben almacenarse en un congelador hasta que vayan a usarse, ya que de lo contrario puede resultar difícil colocarlos en el portaobjeto.

Aclara el Texto Ecuador

3. Détection [19] Tiret 1 ligne 1

Tiret 5, ligne 3

Rédactionnel

De fond

Les thrips peuvent être prélevés un par un sur la plante (feuilles, fleurs ou fruits), et transférés à l'aide d'un pinceau fin humide et déposés dans des microtubes contenant une solution AGA.

- lors d'une infestation de faible ampleur produisant des symptômes difficiles ou impossibles à déceler;

- lorsque les œufs ne sont présents que dans le tissu végétal (par exemple après un traitement extérieur susceptible d'avoir éliminé les stades visibles).

les pièges jaunes peuvent également être utilisés étant également efficaces.



Côte d’Ivoire

3. Détection [19] Rédactionnel Les thrips peuvent être prélevés un par un La compréhension du texte R.D. CONGO,





sur la plante (feuilles, fleurs ou fruits), transférés à l'aide d'un pinceau fin humide et déposés dans des microtubes contenant une solution AGA.

- lors d'une infestation de faible ampleur produisant des symptômes difficiles ou impossibles à déceler;

- lorsque les œufs ne sont présents que dans le tissu végétal (par exemple après un traitement extérieur susceptible d'avoir éliminé les stades visibles).

les pièges jaunes peuvent également être utilisés.

TOGO

3. Détection [19] 1.Tiret 1

2.Tiret 2,Ligne5

3.Tiret 5, ligne3

Rédactionnel Les thrips peuvent être prélevés un par un sur la plante (feuilles, fleurs ou fruits), transférés à l'aide d'un pinceau fin humide et déposés dans des microtubes contenant une solution AGA.

…..thrips ont tendance



3. Detección [19] Redacción Se pueden utilizar diversos métodos para recolectar especímenes de trips (Mantel y Vierbergen, 1996; modificado):– los trips pueden cogerse

individualmente de la planta (hojas, flores o frutos) y transferirse con la ayuda de un pincel fino húmedo a

Aclara la redacción del texto Ecuador





microtubos que contengan AGA.– los trips pueden hacerse caer en

una bandeja de plástico pequeña (por ejemplo, una bandeja blanca, para los especímenes de color oscuro o una bandeja negra para los especímenes de color claro) sacudiendo las partes de la planta. En condiciones relativamente frías, los trips generalmente empiezan a caminar por la bandeja en vez de volar, lo cual dará tiempo para recogerlos con un pincel fino húmedo, mientras que en zonas tropicales la recolección debe realizarse con mayor presteza ya que es probable que los trips salgan volando mucho más rápidamente. Los trips se pueden ver con facilidad en la bandeja utilizando solo una lupa, pero un observador con experiencia también los puede ver fácilmente a simple vista.

– pueden guardarse partes de la planta durante 24 horas en una bolsa de plástico cerrada, con un pedazo de papel de filtro para absorber la condensación. La mayoría de los trips saldrán de las partes de la planta y será posible entonces recolectarlos del interior de la bolsa.

– se puede utilizar un embudo Berlese para procesar material vegetal tal como flores, césped,





hojarasca, musgo e incluso ramas secas de árboles. El embudo contiene un tamiz en el cual se deposita el material vegetal. Debajo del tamiz, el fondo del embudo lleva a un recipiente que contiene etanol al 70-96 %. El embudo se coloca bajo una lámpara eléctrica (60 W) y el calor y la luz harán que cualquier trip que se encuentre presente en las plantas escape hacia abajo y caiga en el recipiente. Después de un período apropiado (8 horas en el caso de flores cortadas), el contenido del recipiente puede observarse en un estereomicroscopio.

– los trips pueden monitorearse (solo los adultos alados) utilizando trampas pegajosas de colores. La capacidad de un color de atraer a los trips varía según las diferentes especies de trips; las trampas azules o blancas son buenas para T. palmi, aunque las trampas amarillas también funcionarán. Para la preparación del portaobjeto y la identificación al microscopio, los trips tendrán que extraerse de las trampas utilizando fluidos para desencolar a base de aceites de cítricos, diclorometano o un sucedáneo de la trementina.

3. Detección [19] Oración 1

Sangría 1

Editorial Se pueden utilizar diversos métodos para recolectar especímenes de trips (Mantel y Vierbergen, 1996; modificado):– los trips pueden cogerse tomarse

individualmente de la planta (hojas, flores o frutos) y transferirse con la ayuda de un pincel fino húmedo a microtubos que contengan AGA.

.....


México





Sangría 3

– pueden guardarse partes de la planta durante 24 horas en una bolsa de plástico cerrada, con un pedazo de papel de filtro para absorber la condensación. La mayoría de los trips saldrán de las partes de la planta y será posible entonces recolectarlos del interior de la bolsa.


3. Detection [19] 2nd indent Editorial Thrips may be beaten off, from plants parts, onto a small plastic tray (e.g. a White tray for dark-coloured specimens or a Black tray for light-coloured specimens).

Clarity Grenada

3. Detection [19] 2nd indent Editorial Thrips may be beaten off, fromplant parts, onto a small plastic tray(e.g. a white tray for dark-colouredspecimens or a black tray for lightcolouredspecimens)


3. Detection [19] Bullet 2 Substantive .....fine brush, whereas in tropical areas warmer conditions collection has to be done more rapidly as the thrips are.....

This should refer to sensitivity to temperature i.e. warmer conditions rather than specific climate i.e. tropical areas.

South Africa

3. Detection [19] 2nd indent, 2nd sentence

4th indent, 1st sentence

Substantial

Technical

In cooler conditions, the thrips usually start walking across the tray rather than flying off, allowing time for the thrips to be picked off with a moist fine brush, whereas in warmer conditions tropical areas collection has to be done more rapidly as the thrips are likely to fly off much more quickly.

A Berlese funnel can be used to process plant material such as bulbs, flowers, turf, leaf litter, moss and even dead branches of trees.

The tropics does not neccessarily include all warm climates. For consistancy also, note that the beginning of the sentence starts with "cooler conditions" and not "temperate areas".

It is also used for bulbs.

Israel

3. Detection [19] 3rd indent Susbtantive Plants parts may be sealed in a plastic bag for 24 hours, with a piece of filter paper enclosed to absorb condensation

Clarity Grenada

3. Detection [19] 3rd indent Substantive Plant parts may be sealed in a plasticbag for 24 hours, with a piece of






filter paper enclosed to absorbcondensation.

3. Detection [19] Indent 4, sentence 3

Substantive …. the bottom of the funnel leads into a receptacle containing 70–96% ethanol 10% alcohol plus wetting agent.

An adjustment to be considered. We would suggest that if 70-96% ethanol is used to collect thrips from berlese funnel, it could be assumed that a morphological identification will be more difficult or impossible because thrips will be more difficult to prepare for slide mounting. In contrast, the use of collecting fluid with 10% alcohol plus wetting agent allows, based on our experience, a good preservation of thrips that remain flexible.

EC

3. Detection [19] Indent 4,sentence 4

Substantive The funnel is placed under an electric lamp (60 W), and the heat and light will cause any most of the thrips present in the plants to escape by heading down towards the receptacle

In order to get the accreditation EN ISO/IEC 17025 for Thrips palmi the Berlese funnel technique was validated at the Diagnostic Centre for Plants (ILVO, Merelbeke, Belgium). There is no certainty that all the thrips can be isolated using the Berlese funnel system.

EC

3. Detection [19] 3rd 4th indent4th row

5th row,1st sentence

Editorial

editorial

...funnel is placed under an electric lamp (60 W) and where the heat and light will cause drive any thrips present…

…in the plants to escape by heading down towards the receptacle.

Better wording

Unnecessary wording

USA

USA

3. Detection [19] Indent 4,sentence 5

Substantive After an appropriate period (e.g. 8 hours for cut flowers), the content of the receptacle …

To indicate that an appropriate period may differ dependent on the type of Berlese funnel and the kind and volume of the plant material. Based on the validation results at the Diagnostic Centre for Plants (ILVO, Merelbeke, Belgium) it was concluded that an isolation time of 20 hours had been necessary to obtain accurate data.

EC

3. Detection [19] Indent 5 Substantive Thrips may be monitored (winged adults only) using coloured sticky traps or other appropriate methods.

Giving more option of other effective methods that can be used.

Seychelles

3. Detection [19] Indent 5,Sentence 3

Editorial For microscope slide preparation and identification, the thrips will have to be removed from the traps using glue-

Chemicals provided in the protocol are not applicable in all countries

EC





removing fluids such as those based on citrus oils, dichloromethane, a turpentine substitute

3. Detection [19] Tiret 1

Tiret 5 Phrase 2

Rédactionnel Les thrips peuvent être prélevés un par un sur la plante (feuilles, fleurs ou fruits), transférés à l'aide d'un pinceau fin humide et déposés dans des microtubes contenant une solution AGA.


La compréhension du texte Togo

3. Detection [20]

4. Identification [21]

4. Identification [22] Ligne 1 Rédactionnel ….morphologique est limitée aux spécimens adultes,…..

Clarité Burundi

4. Identificación [22] Frase 4 Sustancial Para poder identificar....utilizando un microscopio de elevada potencia con un aumento, por lo menos de 400x.

Es necesario añadir un dato más concreto acerca del aumento del microscopio que permita una identificación certera.

Perú

4. Identification [22] Sentence 5 Substantive Be specific on the high power microsope (e.g x400)

For consistence and clarity Kenya

4. Identification [22] Sentence 5 substantive Be specific on the high power microsope For consistence and clarity IASPC-GABON

4. Identification [22] Sentence 5 substantive Be specific on the high power microsope For consistence and clarity Mozambique

4. Identification [22] Sentence 5 substantive Be specific on the high power microsope For consistence and clarity Swaziland4. Identification [22] Sentence 5 substantive Be specific on the high power microsope For consistence and clarity ZAMBIA

4. Identification [22] Sentence 3 substantive Be specific on the specification of high -power microscope to address the size problem for identification

For clarity, the pictures and the diagrams should indicate magnification for easy identification

NIGERIA

4. Identification [22] Sentence 5 Substantive species identification, these must be examined using a high-power microscope.

Be specific about on high power microscope to explain the particular situation for clarity

Seychelles

4. Identificación [22] Oración 4 Editorial La identificación de las especies de trips México





mediante el examen morfológico está limitada a los especímenes adultos debido a que no existen claves adecuadas para la identificación de huevos, larvas o pupas. Sin embargo, la presencia de larvas en las muestras puede proporcionar información adicional importante, tal como la confirmación de su desarrollo en las plantas hospedantes. El método primario de identificación de material adulto se basa en los caracteres morfológicos. Para poder identificar la especie, debe examinarse el material utilizando un microscopio de elevada potencia. El uso de este protocolo junto con preparaciones microscópicas de buena calidad debería permitir identificar con certeza solamente los especímenes de T. palmi adultos mediante el examen morfológico tan solo.


4. Identification [23] Whole paragraph

substantive Molecular assays can be applied to all life stages for which morphological identification to species is not possible. Specificity of molecular assays is limited as they have been developed for specific purposes and evaluated against a restricted number of species, using samples from different geographic regions; therefore, such information needs to be carefully interpreted. Additionally, in cases where adult specimens are atypical or damaged, molecular assays may provide further relevant information about their identity.However, such information needs to be carefully interpreted because the specificity

The tests were carried out on a very limited number of organisms from specific parts of the world. The molecular tests can be used to confirm an organism as Thrips palmi, but they cannot be used as evidence that an organism is not Thrips palmi.

USA





of molecular assays is limited and these assays have been developed for specific purposes and evaluated against a restricted number of species.

4.1 Morphological identification of the adult thrips

[24]

4.1.1 Preparation of thrips for microscopic examination

[25]

4.1.1 Preparation of thrips for microscopic exa

[26] 1st sentence Substantive ...microscopic examination (using at least XX magnification), …

To help countries set up equipment with adequate magnification for identification

Korea

4.1.1 Preparación de trips para el examen microscópico

[26] Redacción Para el examen al microscopio de elevada potencia, los trips adultos deben montarse en el portaobjeto del microscopio. Para guardar el material en una colección de referencia es preferible macerarlo, deshidratarlo y montarlo en bálsamo de Canadá; Mound y Kibby (1998) ofrecen una descripción completa de este proceso. Sin embargo, el protocolo completo para la preparación del portaobjeto destinado al archivo toma tres días para completarse.

Mejora el texto Ecuador


[26] Substantive Material Specimens to be kept in a reference collection is are best macerated, dehydrated and mounted in Canada balsam

Use of more appropriate term Grenada


[26] Sentence 2 Substantive Material Specimens to be kept in a reference collection is are best macerated, dehydrated and mounted in Canada balsam

Use of more appropriate term Trinidad and Tobago

4.1.1 Preparation of thrips for microscopic

[26] 2nd row substaintive Need to be clarifed use of the word “macerated” is questioned

the word “macerated”usually means “to cut up” which does not seem an appropriate

China





exa procedure here.4.1.1 Preparation of thrips for microscopic examination

[26] Sentence 2 Substantive Reference named in the para. Accessebility to users?

Ease of Access for reference purposes Kenya


[26] Sentence 2 substantive Reference named in the para. Accessebility to users????

Ease of Access for reference purposes IASPC-GABON



Ease of Access for reference purposes Mozambique



Ease of Access for reference purposes ZAMBIA


[26] Last sentence Editorial Numbering .. use 3.. to be consistent with para 28

Consistency Korea


[27] Redacción Para las identificaciones rutinarias, un líquido de montaje hidrosoluble como el medio de Hoyer (50 ml de agua, 30 g de goma arábiga, 200 g de clorhidrato, 20 ml de glicerina) resulta más rápido y relativamente económico. A continuación se describe un método popular de preparación de portaobjetos para la identificación de rutina, presentado en Mound y Kibby (1998) (los diferentes laboratorios pueden considerar que otras variantes que funcionan igualmente bien).

Mejora el texto Ecuador


[28] Editorial Transfiera los especímenes del fluido de conservación a etanol al 70% limpio; si los especímenes están razonablemente flexibles, intente desplegar las patas, las alas

Mejora la redacción Ecuador





y las antenas utilizando microalfileres entomológicos; ponga un trips, con la parte ventral hacia arriba, en una gota de medio de Hoyers depositada en un cubreobjeto de 13 mm de diámetro y use microalfileres para colocarlo bien de ser necesario; coloque cuidadosamente un portaobjeto sobre la gota de manera que esta y el cubreobjeto se adhieran re al centro del portaobjeto; dé la vuelta al portaobjeto en cuanto el líquido de montaje se haya extendido hasta los bordes del cubreobjeto; escriba en el portaobjeto los detalles (localidad y fecha de recolección y planta hospedante); introduzca el portaobjeto, con la tapa arriba, en un horno de secado entre 35 y 40oC y espere 6 horas antes de intentar hacer el estudio; deje en el horno aproximadamente 3 semanas para secar el líquido de montaje, antes de sellar el cubreobjeto con resina o esmalte de uñas.

4.1.1 Preparation of thrips for microscopic examination

[28] Sentence 6 Substantive Including locality, altitude of locality, date of collection

It is necessary item Nepal

4.1.2 Identification of the family Thripidae

[29]


[30] First sentence Substantive Thrips palmi belongs to the family Thripidae, which includes approximately 1850more than 2000 species in approximately 260 290 genera

Numbers of species and genera are incorrect (see Mound & Morris, 2007: Zootaxa, 1668)

Japan


[30] Sentence1 substantive Thrips T. palmi No need to write full name in each writing Nepal

Table 1: Family [31]





Thripidae – shared characteristics

Body part [32]

Antennae [33] Sentence 1 Editorial Antennae seven or eight segments (occasionally nine or six) 6-9 segments of antenna

Clarity and consistence Kenya

Antennae [33] Sentence 1 Editorial

editorial 6_9segments of antenna Clarity and consistence IASPC-GABON

Antennae [33] Sentence editorial 6_9segments of antenna Clarity and consistence MozambiqueAntennae [33] Sentence 1 editorial 6_9segments of antenna Clarity and consistence ZAMBIA

Antennae [33] Box 1 editorial (occasionally nine six or six nine) For consistency, numerically six comes before nine.

NIGERIA

Forewings (if fully developed)

[34]

Abdomen – female [35]

Median sternites – male [36] Characteristic Substantive with one or moreor without glandular areas Species within Thripidae don’t always have sternal glandular area.(see Mound & Masumoto (2009) P8 paragraph [Pore plates among Thripinae] line5)

Japan

4.1.3 Identification of the genus Thrips

[37]

4.1.3 Identification du genre Thrips

[38] Ligne 2 De fond Les individus Les membres du genre ont en commun les caractères mentionnés au Tableau 2.


4.1.3 Identification du genre Thrips

[38] Phrase 2 Rédactionnel Les individus du genre ont en commun les caractères mentionnés au Tableau 2.



[38] Rédactionnel Les individus du genre ont en commun les caractères mentionnés au Tableau 2.

La compréhension du texte R.D. CONGO, TOGO

Table 2: Genus Thrips – [39] Substantive Table 2:Genus Thrips – shared Slightly tedious Japan





shared characteristics, adult specimens

characteristics, adult specimens

Body part [40]

4.1.3 [41] The title of the line

Right hand column

Editorial Female wings

macropterous or micropterous female

Error introduced in re-formatting

Clarification (wings are not macro- or micropterous)

EC

Female [41] Substantive Female wings Add wings because the word Female alone is not a body part to be in line with the title featured in Table 2.

Seychelles

Tableau 2 : Genre Thrips – caractères communs

[41] Colonne 1 De fond Femelle Ailes des femelles Compréhension du texte Côte d’Ivoire


[41] De fond Ailes des femelles La compréhension du texte BURUNDI


[41] De fond Ailes des femelles La compréhension du texte R.D. CONGO, TOGO

Antennae [42]

Ocellar setae [43]

Pronotum [44]

Prosternal basantra [45]

Forewings [46] Characteristic Substantive the first vein with variably spaced setal row, on the first vein usually interrupted second vein with complete setal row

Setal row on first vein is not “usually interrupted” and chaetotaxy on second vein is important.(see Nakahara (1994)P14 paragraph [Fore wings])

Japan

Metascutum [47] Characteristic Substantive Add new characteristic in para 47;Fore tibia: apical claw absent

To distinguish from related genera. (see Masumoto & Okajima (2002) P21 [in key to genera] , Zur Strassen (2003)P60-94 [in key to genera])

Japan





Metasternal furca [48]

Tarsi [49]

Abdominal tergites and sternites

[50]

Abdominal tergites [51]

Abdominal sternites and pleurotergites

[52] Characteristic Substantive Add new characters;with or without discal (accessory) setaemale sternites III-VII or less each with a glandular area

Important character state.(see Mound & Masumoto (2005) P10)

Japan


[53]


[54] Sentence 1 Substantive Accessibility of reference Kenya


[54] Sentence 1 substantive Accessibility of reference IASPC-GABON


[54] Sentence 1 substantive Accessibility of reference Mozambique


[54] Sentence 1 Substantive Accessibility of reference NIGERIA


[54] Sentence 1 substantive Accessibility of reference ZAMBIA

4.1.3 Identificación del género Thrips

[54] Editorial La identificación de adultos puede realizarse con claves. Mound y Kibby (1998) proporcionaron una clave para 14 especies de Thrips de importancia económica, incluida Thrips palmi. Además, existe una herramienta de ayuda en CD-ROM para la identificación de trips que incluye un sistema de identificación relativo a 100 especies de la plaga de todo el mundo

Espacio entre palabras Ecuador





basado en fotomicrografías (Moritz et al., 2004).

4.1.3 Identificación del género Thrips

[54] Oración 2 Editorial La identificación de adultos puede realizarse con claves. Mound y Kibby (1998) proporcionaron una clave para 14 especies de Thrips de importancia económica, incluida Thrips palmi. Además, existe una herramienta de ayuda en CD-ROM para la identificación de trips que incluye un sistema de identificación relativo a 100 especies de la plaga de todo el mundo basado en fotomicrografías (Moritz et al., 2004).



[54] Sentence 2 substantive Thrips T. palmi No need to write full name in each writing Nepal


[54] Sentence 4 Substantive .....around the world based on photomicrographs(Moritz et al 2004 and Moritz 2006)

To include the relevant updated/ newer version/ publication

South Africa


[55] 1st line Editorial comprehensive keys to the genus are available and are produced on a regional basis (no such key has been produced for the Afrotropical region):

Clarity Grenada


[55] Sentence 1 Editorial comprehensive keys to the genus areavailable and are produced on aregional basis (no such key has beenproduced for the Afrotropicalregion):



[55] 1st sentence Editorial More comprehensive keys to the genus are available and produced on a regional basis (no such key has been produced for the Afrotropical region):

More clarification Egypt

4.1.3 Identification [55] 1st sentence Editorial More comprehensive keys to the genus are More clarification Lebanon -Tunisia





of the genus Thrips available and produced on a regional basis (no such key has been produced for the Afrotropical region):


[55] Sentence 1 Editorial …the genius is available and produced… Kenya


[55] Sentence 1 Editorial …the genius are available and produced… IASPC-GABON


[55] Sentence 1 Editorial …the genus are available and produced… ZAMBIA


[55] Sentence 1 row 1

Editorial More comprehensive keys to the genus are available and produced on a regional basis.

For clarity NIGERIA


[55] Substantive Add new information after 2nd sentence; Mound & Azidah (2009) provided a key to species of Peninsular Malaysia.

Latest information. Japan


[55] Editorial More comprehensive keys to the genus are available produced on a regional basis (no such key has been produced for the Afrotropical region):

Asia: Bhatti (1980) and Palmer (1992) provide keys for the identification of species of Thrips occurring in the Asian tropics.

Europe: zur Strassen (2003) has produced the most recent comprehensive key to the species of Europe including Thrips (in German).

North, Central and South America: Nakahara (1994) provides a key for Thrips species from the New World. A key to the species of Thrips found in Central America is given by Mound and

To match the description performed for Central America

COSAVECHILEBRAZILPARAGUAYURUGUAYARGENTINA





Marullo (1996) though only one of these species is native to the region. Only species of Thrips that were transported there by the international plant trade are to be found in South America. In South America, native species are not to be found.

Oceania: Mound and Masumoto (2005) provide a key to the Thrips species of Oceania. (The authors of the paper are aware of the error inadvertently introduced on p. 42 in the section “Relationships” whereby a characteristic of T. flavus – ocellar setae III close together behind the first ocellus – is attributed to T. palmi. The correct information is provided in the T. palmi species description immediately above and is illustrated in Figure 72.)


[55] Sentence 9 Editorial Only species of thrips were produced… Kenya


[55] Sentence 9 editorial Only species of thrips were produced… IASPC-GABON


[55] Sentence 9 editorial Only species of thrips were produced… ZAMBIA


[55] rows 9 & 10 last sentence

editorial Only species of Thrips that were transported introduced there by……………… South America.

For clarity NIGERIA

4.1.4 Identification of Thrips palmi

[56]

4.1.4 Identification [57] Editorial Morphological characteristics of Thrips Italics were on the normal words and T. Israel





of Thrips palmi palmi palmi was not in italics. 4.1.4 Identification of Thrips palmi

[57] 1st row Editorial “Thrips palmi” to “Thrips palmi” China APPPC


[57] Sentence 1 Morphological characteristics of Morphological characteristics of Thrips palmi Thrips palmi

Why italics?/ scientific name of insect Nepal


[57] Editorial Morphological characteristics of Thrips palmi Morphological characteristics of Thrips palmi

Italic scientific name for conformity and consistence

Seychelles


[57] Editorial Morphological characteristics of Thrips palmi

The scientific name must be in italic Egypt


[57] Editorial Morphological characteristics of Thrips palmi

The scientific name must be in italic Lebanon -Tunisia

4.1.4 Identification de Thrips palmi

[57] Ligne 1 Rédactionnel Caractères morphologiques de Caractères morphologiques de Thrips palmi

Pas en italic, pour être en conformité avec les normes scientifiques de nomenclature

Côte d’Ivoire


[57] Rédactionnel Caractères morphologiques de Thrips palmi Etre en conformité avec les normes scientifiques de nomenclature

BURUNDI


[57] Rédactionnel Caractères morphologiques de Thrips palmi Etre en conformité avec les normes scientifiques de nomenclature

R.D. CONGO


[57] Sentence 1 Editorial 4.1.4.1 Morphological characteristics of Thrips palmi

1. Formatting: The heading should be in normal font whilst the species name should be in italics

2. It is also recommended that this heading be numbered for easier reference

South Africa


[58] Line 2 editorial Sakimura Sakamura et al (1986) For consistency NIGERIA


[59] Ligne 4 De fond Ainsi, chez T. palmi la coloration antennaire peut être différente différer de l'état observé

Pour éviter la confusion Côte d’Ivoire





le plus fréquemment tout comme le nombre de soies distales sur l'aile antérieure.


[59] Ligne 4 De fond Ainsi, chez T. palmi la coloration antennaire peut être différente de l'état observé le plus fréquemment tout comme le nombre de soies distales sur l'aile antérieure.

Pour éviter la confusion BURUNDI


[59] 3rd sentence De fond Ainsi, chez T. palmi la coloration antennaire peut être différente de l'état observé le plus fréquemment tout comme le nombre de soies distales sur l'aile antérieure.

Pour éviter la confusion R.D. CONGO, TOGO

Table 3: A list of morphological characters that collectively distinguish Thrips palmi from other species in the genus Thrips

[60] For relevant tables

Editorial Would be useful to have the figures that illustrate these characters cross referenced in the table against each line to assist the diagnostician.

Australia


[60] Sentence 1 Editorial Table 3: A list of morphological characteristics characters that collectively distinguish Thrips palmi from other species in the genus Thrips

For consistency and clarity Seychelles


[60] Sentence 1 Editorial A list of morphological characters characteristics ...

Consistence and clarity with other tables in the document

Kenya

Table 3: A list of morphological characters that collectively distinguish Thrips palmi

[60] Sentence 1 Editorial Table 3: A list of morphological characteristics characters that collectively distinguish Thrips palmi from other species in the genus Thrips

Consistence and clarity with other tables in the document

Swaziland





from other species in the genus Thrips


[60] Sentence 1 Editorial Replace character with characteristics Consistence and clarity with other tables in the document

IASPC-GABON


[60] Sentence 1 Editorial Replace character with characteristics Consistence and clarity with other tables in the document

ZAMBIA


[60] Sentence 1 substantive Thrips T. palmi No need to write full name in each writing Nepal

Cuadro 3: Lista de caracteres morfológicos que colectivamente distinguen Thrips palmi de otras especies del género Thrips

[60] Editorial Cuadro 3: Lista de caracteres morfológicos que colectivamente distinguen a Thrips palmi de otras especies del género Thrips

Nombre científico con letra cursiva Ecuador


[61]






[62] Sentence 1Row 1

editorial A clear yellow body with no dark areas on the head, thórax, or abdomen with (slightly thickened blackish body setae): ……………………, prominent setae dark

For clarity

NIGERIA


[63]


[64]


[65] Oración 1 Editorial La seta ocelar III se encuentra bien justo fuera del triángulo ocelar, o bien tocando las líneas tangentes que conectan el ocelo anterior y cada uno de los ocelos posteriores



[66] Oración 1 Editorial Metaescutelo con esculturación convergente en la parte posterior; par de setas centrales detrás del margen anterior; presencia de un par de sensilas campaniformes presente


Table 3: A list of morphological characters that collectively

[67]





distinguish Thrips palmi from other species in the genus ThripsTable 3: A list of morphological characters that collectively distinguish Thrips palmi from other species in the genus Thrips

[68]


[69]


[70]


[71] Morphological character

Editorial two pairs of pores (campaniform sensilla)two pairs of campaniform sensilla pores (pores campaniform sensilla)

Order of terms should be standardized.(See para 47)

Japan


[72]







Substantive Add new characters;Abdominal pleurotergites without discal setae, ciliate microtrichia.

Important character state. (See prar111) Japan



Substantive Add new characters;Male sternites III–VII each with a narrow transverse glandular area, tergite VIII with posteromarginals comb developed.

Important character state.(see Mound & Masumoro (2005)P42)

Japan


[75] Editorial This line is similar to paragraph 95 – is one of these redundant? Para 95 could be deleted

Australia


[76] Sentence 1 Editorial 4.1.4.2 Comparison with similar species (species that are yellow without darker markings, or predominantly yellow, or sometimes yellow)

1. Formatting: The heading should be in normal font

2. It is also recommended that this heading be numbered (and follow numbering in para 57) for easier reference

South Africa


[76] Sentence 1 editorial Comparison with similar…..comparison with similar…….

No italics in needed Nepal


[76] All para Editorial Remove italics consistence Kenya


[76] All para Editorial Remove italics consistence IASPC-GABON


[76] All para Editorial Remove italics consistence ZAMBIA


[76] Whole sentence Editorial Remove italics For consistency NIGERIA






[76] Sentence 1 Substantive Comparison with similar species (species that are yellow without darker body markings, or predominantly yellow, or sometimes yellow)

The sentence is not clear at this place of text, clarification is needed

Egypt


[76] Substantive Comparison with similar species (species that are yellow without darker body markings, or predominantly yellow, or sometimes yellow)

The sentence is not clear at this place of text, clarification is needed

Lebanon -Tunisia


[77]


[78] Whole sentence Substantive The pest name are to be followed by authors name and the year of publication

For clarity NIGERIA


[78] Sentence 1 Substantive Reflect aothor and year of identification for each organism

Kenya


[78] Sentence 1 substantive Reflect aothor and year of identification for each pest

IASPC-GABON


[78] Sentence 1 substantive Reflect aothor and year of identification for each pest

ZAMBIA


[78] Sentence 1 Substantive Two Indian species (T. alatus Bhatti and T. pallidulus Bagnall) are very similar to T. palmi, although little is known about their biology.

Reflect the author and year of identification of the 2 species for reference

Seychelles


[79] Sentence 1 Substantive Reflect the autor and year of organism identification

Kenya


[79] Sentence 1 substantive Reflect the autor and year of pest identification

IASPC-GABON


[79] Sentence 1 Substantive Reflect the autor and year of pest identification.

NIGERIA


[79] Sentence 1 substantive Reflect the autor and year of pest identification

ZAMBIA






[79] Sentence 1 editorial Thrips alatus T.palmi No need to write full name in each writing Nepal


[80] 4th indent Substantive Add new area;distribution: India, Nepal, Malaysia.

Latest information. (See Malaysia by Mound & Azidah (2009) P57)

Japan


[81] Sentence 1 editorial Thrips pallidulus T.pallidulus No need to write full name in each writing Nepal


[82]


[83]


[84] Sentence 1 editorial Thrips flavus T.flavus No need to write full name in each writing Nepal


[85]


[86]


[87] 5th indent Substantive Distribution: common leaf-feeding species, sometimos a pest of plants in the family Asteraceae (formerly Compositae); Asia, East Africa, Europe, North America, Oceania.

Family Compositae has been reclassified Grenada


[87] 5th Indent Substantive distribution: common leaf-feeding species, sometimes a pest of plants in the family Compositae Asteraceae ( formerly Compositae) ; Asia, East Africa, Europe, North America, Oceania.

Family Compositae has been reclassified Trinidad and Tobago


[87] Bullet 5 Editorial ... East Africa, Europe, North America and Oceania.

Add “and” between the last two areas listed. South Africa


[88] Sentence 1 editorial Thrips tabaci T.tabaci No need to write full name in each writing Nepal






[89] 3rd indent Substantive metascutum with irregular longitudinal reticulations, usually with having internal small wrinkles medially, and no campaniform sensilla

Small wrinkles are in the reticulations. (see Nakahara (1994)P121)

Japan


[90]


[91] Sentence 1 editorial Thrips alni T.alni No need to write full name in each writing Nepal


[92]


[93] Sentence 1 editorial Thrips urticae T.urticae No need to write full name in each writing Nepal


[94] Add new 5th indent

Substantive Add new characters after last indent;metascutum with longitudinal reticulations medially

Important character state. (see Zur Strassen (2003) P158-159 (in identification key & fig. 459))

Japan


[95] Editorial Delete Seems to be similar to para 75. Suggest deletion.

Australia


[95] 1st sentence Editorial This is designed to be used in conjunction with the figures indicated in the table.

Clarity Grenada


[95] Sentence 1 Editorial This is designed to be used in conjunction with the figures indicated in the table.



[95] Sentence 1 editorial Thrips palmi T.palmi No need to write full name in each writing Nepal

Table 4: Simplified checklists of the diagnostic features for quick recognition: (a) the genus Thrips; (b) Thrips palmi


Table 4: Simplified [96] Substantive Additional references to be used for quick An additional reference is proposed that may South Africa





checklists of the diagnostic features for quick recognition: (a) the genus Thrips; (b) Thrips palmi

recognition of diagnostic features:

CD:PEST THRIPS OF THE WORLD. Moritz G., Mound, L.A., Morris, D.C., Goldarazena, A. Lucid, 2004 Bayer Cropscience, , CSIRO

be useful.


[96] Substantive Delete table 4 Thrips palmi can be distinguished from other Thripidae by Table 2-3.

Japan


[97]


[98]


[99] Add to end of sentence

substantive Add “Pair II is shorter than pair III “ Accuraty China

Table 4: Simplified checklists of the diagnostic features for

[99] Substantive with two pairs of ocellar setae (II and III); pair I missingabsent, pair II shorter than pair III

Length of pair II is an important character state. (see Mound & Masumoto (2005)P10)

Japan





quick recognition: (a) the genus Thrips; (b) Thrips palmiTable 4: Simplified checklists of the diagnostic features for quick recognition: (a) the genus Thrips; (b) Thrips palmi

[100] Substantive Move to the para 108. This part is character state of palmi, not of genus Thrips. (see Mound & Masumoto (2005)P10)

Japan


[101] Substantive Delete This character state is not important to recognize genus Thrips. (see Mound & Masumoto (2005)P10,Nakahara (1994)P14 paragraph [Fore wings])

Japan


[102]


[103]


[104]






[105]


[106]


[107]


[108] Substantive with their bases sited outside of the ocellar triangle or touching the tangent lines connecting the anterior ocellus to each of the posterior ocelli

Not neccesary. Japan


[109] Add new paragrap after [108]

Substantive Add new paragraph 108 between and 109 “Pronotum with two pairs of major posteroangular setae”

Important character state.(see Mound & Masumoto (2005) P42)

Japan

Table 4: Simplified checklists of the diagnostic features for

[110] Substantive with median pair of setae behind the anterior margin and a pair ofpaired campaniform sensilla; with striate sculpture

Position of median pair of setae is important to identify species. (see Mound &Masumoto (2005)P42)

Japan





quick recognition: (a) the genus Thrips; (b) Thrips palmi

converging posteriorly


[111] Substantive discal setae absent; lines of sculpture without numerous ciliate microtrichia

“ciliate” expresses this character state more appropriate than “numerous”. (see Nakahara (1994) P121)

Japan


[112]


[113]


[114]


[115] Editorial with two pairs of pores (anterior and posterior) pairs of campaniform sensilla (pores)

Arrangement of pores (campaniforn sensilla) is unclear. Order of terms should be standardized. (see para 47)

(see paragraph 47)

Japan






[116] Add new indent after [116]

Substantive Male: tergite VIIIsternites

/posteromarginals comb developed

This character state is important to distinguish this species from similar species.(see Mound & Masumoro (2005)P42)

Japan

Figura 4. Ubicación de los caracteres generales de Thrips (hembra, vista dorsal)

[117] Entre Párrafo [116] y [117]

Sustancial Cuadro 5: Clave de Identificación para las especies de la Región enfatizando las especies relacionadas a Thrips Palmi

El uso de una clave de identificación es sencillo de manejar. Además, el especialista de la región debe ser capaz de diferenciar, tanto a Thrips Palmi como a las especies abundantes en la región, por lo que es conveniente anexar la Clave en un cuadro

Perú

Figure 4: [117] Heading Repetition GrenadaFigure 4: [117] Heading Editorial 4: Location of general characters

of Thrips (female – dorsal view)Repitition Trinidad and

TobagoFigure 4: [117] title editorial Remove the upper heading consistence NIGERIA

Figure 4: [117] Sentence 1 Editorial Title should be at the bottom of figure consistent in all the figures KenyaFigure 4: [117] Sentence 1 editorial Title should be on top of figure consistence IASPC-GABON

Figure 4: [117] Sentence 1 editorial Title should be on top of figure consistence SwazilandFigure 4: [117] Sentence 1 editorial Title should be on top of figure consistence ZAMBIA

Figure 5 (Figs 5.1 to 5.12

[118] All photo images

Editorial While the images are reasonable, higher quality photographs exist and are freely available that demonstrate identifying features of the Thrips. Images for identification of T. palmi are on the Australian page of the IPP. IPPC is welcome to use these in the standard or reference them as appropriate to assist diagnosticians.

Australia

Figure 5 (Figs 5.1 to 5.12






Fig. 5.1(a), (b): [119]

Fig. 5.2(a), (b): [120] Photo and drawing

Editorial Modify line drawing to correlate with photograph

Antennal segments IV and V are illustrated in 5.2a but antennal segments III and IV are illustrated in the drawing

Australia

Fig. 5.2(a), (b) [120] 5.2(a) Editorial To align the title of and the indications in the figure.

Referring to segments III and IV in the title, while depicting segments IV and V creates some confusion.

EC

Fig. 5.2(a), (b) [120] 5.2(b) Substantive The drawing is not technically correct. The forked sense cones arise from the opposite sides of the antennal segments, so their insertions should not be depicted as visible on the same picture, as it could be confusing.

EC

Fig. 5.2(a), (b): [120] Fig.5.2 (b) Substantive Figure incorrect Sense cone of IV is situated dorsally in the fig but actually it is situated ventrally.(see Fig5.1 (b) in this article and specimen)

Japan

Fig. 5.3(a), (b): [121] Fig 5.3 (a) substantive Replace fig with an image where the ocellar setae II can be seen clearly or indicate the location of the setae II in the current image

The position of ocellar setae III are obvious in fig 5.3.(a)the position of ocellar setae II is not clear. This character is mentioned in Table 4 (page 9 para 99)

New Zealand

Fig. 5.4(a), (b): [122] 5.4(a) Substantive Replacement of the picture We offer another picture for a possible replacement of the picture 5.4(a) as it seems not to have an optimal field depth; this can confuse a non specialist. See the embedded picture : pronotum_2b.jpg (Source : LNPV – France)

Replacement 5.4(a)

EC





Fig. 5.4(a), (b): [122] Fig.5.4 (a) Editorial A short line on photo should be deleted. JapanFig. 5.5(a), (b): [123]

Fig. 5.6(a)–(c): [124] Fig.5.6 (a),(b) Substantive Move to “Fig.5.12”.Fig. 5.6(a), 5.6(b)“ctenidia” and “spiracle” shoud be indicated on the photo.

Other characters of male are shown in Fig.5.12.

Japan

Fig. 5.6(a)–(c): [124] Fig.5.6 (c) Substantive An arrow from “ctenidium” to figure should be slightly moved below.

This arrow does not indicate “ctenidium” Japan

Fig. 5.6(a)–(c): [124] 5.6(c) Editorial It seems there is a printing problem.Check the position of the arrow to exactly point on ctenidium in a printed document.

The arrow that shows ctenidium is in different positions on screen and in a printed document.

EC

Fig. 5.7(a)–(e): [125] Editorial Would be useful to have the relevant characteristic more clearly identified perhaps by an arrow or other pointing it out.

Improved clarity Australia

Fig. 5.8(a)–(c):: [126]

Fig. 5.9(a), (b): [127]

Fig. 5.10(a), (b): [128] 5.10(a) Substantive Replacement of the picture We offer another picture for a possible replacement of the picture 5.10(a).See the embedded picture tergites_1.jpg (Source : LNPV – France)

Replacement 5.10(a)

EC

Fig. 5.10(a), (b): [128] Fig. 5.10 (a) Substantive Suggestion/Requesting replacement of figure 5.10 (a)

Could the visibility of figure 5.10(a) be improved?

The setae (S1) should be visible.

South Africa

Fig. 5.10(a), (b): [128] Editorial 5.10 (b) – the labels S1, S2 and S3 are difficult to see on the line drawing. Suggest

Improved clarity Australia





making bigger or use an alternative way of demonstrating the diagnostic feature

Fig. 5.11(a), (b): [129] B Substantive campaniform sensilla not visible IsraelFig. 5.11(a), (b): [129] Oración 1 Sustancial Fig. 5.11(a), (b): Abdominal tergite IX, two

pairs of campaniform sensilla

Fig. 5.11(a), (b): Tergito Abdominal IX, dos pares de sensilas campaniformes

Faltó de traducir (pero hicieron muy buen trabajo)

México

Fig. 5.12(a)–(c): [130] 5.12(b) and 5.12(c)

Editorial To number sternites on pictures 5.12(b) and/or 5.12(c)

We would see it as an improvement if sternites were numbered. Glandular areas are on sternites III to VII, but for non-specialists to number sternites is not an easy task

EC

Fig. 5.12(a)–(c): [130] Fig.5.12 (b) Editorial Deleted There is same photo at 5.12(c). Japan

4.2Molecular assays for identifying Thrips palmi


4.2 Molecular assays for identifying Thrips palmi

[132] 1st row editorial Change “discrimination” to “identification” Terms as used in the title of the section. China


[132] 1st sentence editorial …in the identification of …. Term as used in the title of the section Korea


[132] 1st sentence

Add at the end of the paragraph

Substantive

substantive

Four molecular assays have been published that can be used in the discrimination to support a morphological identification of T. palmi and these are described below.

A positive identification using molecular techniques is confirmatory; however, a

Extensive use of molecular techniques in plant quarantine will give rise to endless splits and “new” species. Morphologically and behaviorally similar species may be split into other species, creating quarantine pests where there are now none.

Countries should not feel compelled to use molecular methods in cases like this. The

USA

USA





negative result does not exclude the possibility of positive identification. Morphological identification is considered adequate.

morphological methods should be sufficient.

4.2 Ensayos moleculares para la identificación de Thrips palmi

[132] Editorial Se han publicado cuatro ensayos moleculares que se pueden utilizar para identificar T. palmi, los cuales se describen a continuación. La especificidad de cada ensayo se describe también. Este indica la especie de trips respecto de la cual se evaluó cada ensayo y el uso original para el cual se diseñó el ensayo. También existe un sistema de identificación en CD-ROM que incluye datos moleculares sobre diversas especies de trips (Moritz et al., 2004).

Espacio entre palabras Ecuador


[133]


[134]


[135]


[136]

4.2 Ensayos moleculares para la identificación de Thrips palmi

[137] Sangría 1 y sangría 2

Sustancial El ADN puede extraerse de los huevos individuales, adultos, pupas o larvas. Respecto de cada uno de los ensayos descritos a continuación, consúltese en la fuente impresa la técnica concreta de extracción del ADN utilizada originalmente. Los laboratorios pueden considerar que hay técnicas alternativas de extracción igualmente eficaces; el ADN puede extraerse utilizando cualquier método de extracción de ADN adecuado para insectos. Por ejemplo: – los trips pueden molerse en un

México





tampón buffer de lysis en un microtubo, utilizando un micromartinete micropistilo, y el homogeneizado puede recogerse con un equipo de extracción de ADN a base de una proteinasa-K de acuerdo con las instrucciones del fabricante apropiado.

- otra posibilidad es que se muelan el los trips en 50 l de agua libre de nucleasas antes de añadir 50 l de un compuesto 1:1 (volumen por volumen) de resina Chelex 100 y agua libre de nucleasas, calentado a 95ºC durante 5 min y centrifugado a 11.000 g durante 5 min. El sobrenadante se transfiere a un microtubo nuevo y se almacena a −20ºC.

No se están utilizando los términos más adecuados. “Buffer” se utiliza en español como tal buffer y “micropestle” se traduce como micropistilo

Lo normal es que sean molidos más de un trips, faltó una “s” al final de la palabra.


[137] Sentence 10 Editorial Write capital g to read as thus: 11,000 g G …

Kenya


[137] Sentence 10 Editorial Write capital g to read as thus: 11,000G… IASPC-GABON


[137] Sentence 10 Editorial Write capital g to read as thus: 11,000G… Consistence Swaziland


[137] Sentence 10 Editorial Write capital g t or read as thus: 11,000G… ZAMBIA


[137] Indent 2Last sentence

editorial Convert the 11000g to its equivalent in rpm clarity NIGERIA


[138]

4.2.1 SCAR marker-generated sequence-

[139]





based real-time PCR assay for Thrips palmi

4.2.1 SCAR marker-generated sequence-based real-time PCR assay for Thrips palmi

[140]


[141]


[142]


[143] Footnote Substantive Use desclaimer for trade brands Not to be in favor of any agent or manufacturer of products

Kenya


[143] footnote substantive Use desclaimer for tradebrands with IASPC-GABON


[143] Foot note Substantive Use disclamer for trade brands NIGERIA


[143] Footnote substantive Use disclaimer for trade brands with ZAMBIA

4.2.2 COI sequence-based real-time PCR assay for Thrips palmi

[144] Para: 139, 144, 146, &156 and149,

Substantive No abbreviations in the title Kenya


[144] Para: 139, 144, 146, &156 and149,

substantive No abriviations in the title IASPC-GABON

4.2.2 COI sequence-based real-time PCR

[144] Para: 139, 144, 146, &156

substantive No abbreviations in the title ZAMBIA





assay for Thrips palmi and149,4.2.2 COI sequence-based real-time PCR assay for Thrips palmi

[144] Para: 139, 144, 146, &156 and149,

substantive Write COI in full not abbreviation Clarity Swaziland




[145]


[146]




[148] 1st sentence Editorial The 25 l reaction mixture contained 12.5 l of 2X× TaqMan Universal Master Mix (Applied Biosystems) 3, 0.9 M each primer, 0.1 M TaqMan probe, 1.0 l DNA.

(See Kox(2005)) Japan

4.2.3 ITS2 sequence-based PCR-RFLP assay for nine species of thrips including Thrips palmi

[149]


[150]


[151]

4.2.3 ITS2 sequence-based PCR-RFLP assay

[152]

3





for nine species of thrips including Thrips palmi4.2.3 ITS2 sequence-based PCR-RFLP assay for nine species of thrips including Thrips palmi

[153] 2nd sentence

4th sentence

Editorial

Editorial

The 20 l reaction mixture was composed as follows: 1 μM each primer, 250 μM dNTPs, 1 Unit of AmpliTaq Gold DNA polymerase (Applied Biosystems) 5, 2 μl 10 X× reaction buffer [with 25 mM MgCl2], 0.5 µl DNA.

The DNA fragment size of PCR products were analysed by agarose gel electrophoresis.

(See Toda and Komazaki(2002)) Japan

4.2.3 Ensayo de RCP-PLFR basado en la secuencia ITS2 relativo a nueve especies de trips incluido Thrips palmi

[153] Oración 2 Sustancial T. palmi genera un producto de RCP de 588 pares de bases (bp) (de las otras especies se produjeron fragmentos más largos o más cortos). Los 20 l de mezcla de reacción estaban compuestos de: 1 μM de cada iniciador, 250 μM de dNTPs, 1 unidad de AmpliTaq Gold DNA polimerasa (Applied Biosystems)1, 2 μl de tampón buffer de reacción 10X (con 25 mM de MgCl2), 0,5 µl de ADN. La RCP se realizó en un termociclador ADN 9600 (Applied Biosystems)2 con las siguientes condiciones: 9 min a 95oC, 35 ciclos de 1 min a 94oC, 30 s a 50oC, y 1 min a 72oC, seguido de una extensión final de 7 min a 72oC y enfriada rápidamente a temperatura ambiente. Los productos de la RCP se analizaron por electroforesis en gel de agarosa.

No se está utilizando el término más adecuado. En español no traducimos la palabra buffer, la utilizamos como tal: “buffer”. Es un anglicismo muy utilizado comúnmente.

México

4.2.3 ITS2 sequence-based PCR-RFLP assay for nine species of thrips including Thrips

[154] Row 1 Editorial 5 µlL of PCR product Typing error. EC

5

1,6 El uso en este protocolo de diagnóstico de la marca Applied Biosystems en relación con la AmpliTaq Gold DNA polimerasa y con el ABI Prism 7700 o el ABI 7900HT Sequence Detection Systems no implica su aprobación ni la exclusion de otras marcas que también puedan ser adecuadas. Esta información se ofrece únicamente para ayudar a los usuarios de este protocolo y no constituye un aval por parte de la CMF del producto químico, el reactivo o el equipo mencionados. Pueden usarse otros productos equivalentes si se demuestra que producen los mismos resultados.2





palmi4.2.3 ITS2 sequence-based PCR-RFLP assay for nine species of thrips including Thrips palmi

[154] 2nd sentence Editorial Digested PCR products were separated by 2.0 % agarose gel electrophoresis.

(See Toda and Komazaki(2002)) Japan


[155]

4.2.4 COI sequence-based PCR-RFLP assay for ten species of thrips including Thrips palmi



[157]


[158]


[159] 1st sentence Editorial The PCR primers (located in the mitochondrial COI gene sequence) used in this assay are as follows:

(See Brunner(2002)) Japan


[160] Row 1 Substantive To check whether correctly taken from Brunner et al.

Brunner paper checked: fragment length was not correctly taken. The whole PCR product is not 433, but 485 bp long (433 bp is the length of the fragment that was used for sequence analysis, not RFLP analysis)

EC


[160] 4th sentence Editorial To gauge the fragment size produced after amplification, 5 µl of the PCR products were analysed by 1.0-2.0 % agarose gel electrophoresis.


4.2.4 Ensayo de RCP-PLFR basado en la secuencia COI para 10

[160] Oración 2 Sustancial Estos iniciadores amplifican un fragmento de 433 bp en todas las especies separadas por este ensayo. Los 50 l de mezcla de

No se está utilizando el término más adecuado. En español no traducimos la palabra buffer, la utilizamos como tal:

México





especies de trips incluido Thrips palmi

reacción estaban compuestos de: 0,76 μM de cada iniciador, 200 μM de dNTPs, 1 unidad de Taq ADN polimerasa, 5 l de tampón buffer de reacción 10X (con 15 mM de MgCl2), 1 µl de ADN. La RCP se realizó en un termociclador estándar con las siguientes condiciones: 1 min a 94oC, 40 ciclos de 15 s a 94oC, 30 s a 55oC, y 45 s a 72oC, seguido de una extensión final de 10 min a 72oC y enfriada rápidamente a temperatura ambiente. Para medir el tamaño del fragmento producido después de la amplificación, se analizaron 5 µl de producto de la RCP por electroforesis en gel de agarosa

“buffer”. Es un anglicismo muy utilizado comúnmente.


[161]


[162] Sau3AI fragment sizes

Substantive To check whether correctly taken from Brunner et al.

Brunner paper checked: first fragment size not correctly taken. The AluI RFLP fragments are 293 (instead of 93), 104, 70 and 18 bp.

EC


[162] 1st sentence Editorial Restriction fragment sizes produced by T. palmi when the COI fragment is digested with AluII and Sau3AI3AI are as follows:AluI: 291 and 194 bpSau3AI: 293, 104, 70 and 18 bp.


5. Records [163]

5. Registros [164] Frase 1 Editorial Deberían Deben conservarse….. Debería es una forma muy vaga del verbo. Debe, por el contrario, connota carácter de obligatoriedad y ya que se trata de normas estandarizadas, la forma imperativa es más coherente.

Perú

5. Registros [165] Frase 2 Sustancial En los casos …. durante un año, por razones _______________________

Para sustentar ¿Cuáles son las razones por las que se fijó el periodo de un año? Adicionar las razones del tiempo establecido

Perú

5. Registros [165] Oración 1 Editorial En los casos en los que otras partes contratantes puedan verse afectadas en

Sobra una coma antes del punto final. México





forma desfavorable por el diagnóstico, los registros y las muestras (en particular, especímenes preservados o montados en portaobjeto, fotografías de estructuras taxonómicas distintivas, extractos de ADN y fotografías de geles, según corresponda) deberían conservarse durante un año, .

5. Records [165] Add sentence at the end of the paragraph

substantive Genetic sequences obtained during the diagnostic process should be submitted to GenBank or a similar public repository.

Genetic sequences should be submitted to GenBank or similar genetic repository to preserve DNA.

USA

6. Contact points for further information

[166]

6. Contact points for further information

[167]

7. Acknowledgements [168]

7. Acknowledgements [169]

8. References [170] Substative FAO should assist the NPPOs in having Access to all the listed references

For easy Access to information NIGERIA

8. References [170] Substantive Also add to the reference listMoritz, G. (2006) Thripse . Fransenflügler, Thysanoptera. Pflanzensaftsaugende Insekten. Band 1 . Westarp Wissenschaften Verlagsgesellschaft, Hohenwarsleben, Germany. ISBN 3-89432-891-6. Softback, 350 pages, 105 illustrations.

To be included as an additional reference.

It refers to addition made in section 4.1.3 Identification of the genus Thrips (paragraph 54).

South Africa

8. References [170] Sentence 9 Editorial Brunner…… Reference not cited in text Nepal

8. Referencias [170] Entre Frase 9 y 10

Sustancial Mound Laurence.1994-2009 Thysanoptera (thrips) of the World- a checklist http://anic.ento.csiro.au/worldthrips/taxon-details.asp? BIOTA ID=8270[consulta sábado 1 de Agosto de 2009.

Cita del Género Mictothrips Perú

8. References [170] Sentence17 Editorial Mantel… Reference not cited in text Nepal

8. References [170] Sentence 24 Editorial Mound….. Reference not cited in text Nepal8. References [170] Sentence 29 Editorial Mound, L.A. & Morries, D.C. 2007. A new

thrips pest of Myoporum cultivars in Califirnia, in a new genus of …

Edit typo New Zealand

8. References [170] Sentence 50 Editorial Zur Strassen Reference not cited in text Nepal

http://anic.ento.csiro.au/worldthrips/taxon-details.asp

http://anic.ento.csiro.au/worldthrips/taxon-details.asp

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