table of contents - welcome | iowa state university · 2015. 12. 21. · selected papers and...
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Table of Contents
Table of Contents .......................................................................................................................................... 2
VDPAM Organizational Chart ....................................................................................................................... 4
VDPAM External Advisory Board .................................................................................................................. 5
VDL Faculty List ............................................................................................................................................ 6
VDL Staff List ................................................................................................................................................ 8
Faculty Areas of Expertise ............................................................................................................................ 7
VDL Staff List ................................................................................................................................................ 8
2008 Statistical Data ................................................................................................................................... 13
Statistical Trends ......................................................................................................................................... 16
Laboratory Section Overviews .................................................................................................................... 17
Bacteriology ............................................................................................................................................ 17
Bioanalytical Services (BAS) .................................................................................................................. 27
Chemistry/Toxicology (Chem/Tox) ......................................................................................................... 27
Molecular Diagnostics ............................................................................................................................. 32
Pathology ................................................................................................................................................ 33
Serology .................................................................................................................................................. 33
Virology ................................................................................................................................................... 36
Honors and Awards ..................................................................................................................................... 40
Selected Papers and Presentations ............................................................................................................ 40
Glossary ...................................................................................................................................................... 51
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ISU VDL Mission Statement
It is the mission of the Iowa State University Veterinary Diagnostic Laboratory to provide comprehensive and cutting edge diagnostic services to veterinarians, producers, and animal owners in Iowa and nationally. The lab is responsible for delivering accessible, timely, accurate, valid, and consistent test results to aid in the protection of animal and human health. Other services include a wide range of surveillance testing for early detection and identification of foreign animal and emerging domestic disease agents, as well as acts of bioterrorism directed at human and livestock populations and agricultural food supplies. The VDL also provides educational opportunities to professional and graduate students, as well as local, national and international scientists, diagnosticians and practitioners. Research is an important component of the tripartite mission as faculty and staff develop state-of-the-art diagnostic tools and techniques and also direct studies which provide new insights and deeper understanding of pathogenesis, transmission, and immunomodulation of infectious diseases.
The Iowa State University Veterinary Diagnostic Laboratory (VDL) is accredited as a full-service laboratory by the
American Association of Veterinary Laboratory Diagnosticians (AAVLD)
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VDPAM Organizational Chart
5
VDPAM External Advisory Board Name Title/Position Company/Clinic City
Dr Pat Halbur Chair of VDPAM, Executive Director of the ISU VDL
ISU College of Vet Med, VDPAM
Ames, IA
Dr James West Director of Food Supply Veterinary Services (FSVS)
ISU College of Vet Med, VDPAM
Ames, IA
Dr Lorraine Hoffman
Director of Operations, ISU VDL ISU College of Vet Med, VDPAM
Ames, IA
Dr Duane Acker Former Undersecretary of Agriculture, former University President, former Dean, former Department Chair
ISU Animal Science Collaborating Professor
Atlantic, IA
Dr Mark Brinkman Progressive practitioner with swine, beef, and small animal focus
Veterinary Medical Center
Williamsburg, IA
Kevin & Terri Carstensen
Chair of the Iowa Beef Industry Council - Terri President of the ICA - Kevin Beef producers
Iowa Beef Industry Council, Iowa Cattlemen’s Association
Odebolt, IA
Dr Jerry DeWitt Director of the Leopold Center for Sustainable Agriculture
ISU Dept of Ag Ames, IA
Dr Ann Garvey State Public Health Veterinarian Iowa Dept. of Public Health
Des Moines, IA
Dr John Hicks Progressive practitioner with swine and beef focus
Carroll Veterinary Clinic
Carroll, IA
Steve Kerns Immediate Past President IPPA, pork producer, seedstock producer
Kerns Farms Clearfield, IA
Dr W. Eugene Lloyd
Toxicologist, Entrepreneur, lead donor of the W. Eugene and Linda Lloyd Veterinary Teaching Hospital at the ISU CVM
Lloyd, Inc Shenandoah, IA
Eric Lyons Dairy producer Toledo, IA Dr Rodger Main Murphy Brown Western Operations,
integrated food supply company Prestage-Stoecker Farms, Murphy Brown
Ames, IA
Jim McKnight Iowa Farm Bureau, Board Member, beef and swine producer
Iowa Farm Bureau Afton, IA
Bill Northey Iowa Secretary of Agriculture Department of Agriculture & Land Stewardship
Des Moines, IA
Lucy Norton Iowa Renewable Fuels Association-Managing Director
Iowa Renewable Fuels Association
Johnston, IA
Dr Jodi Pettit Progressive practitioner with swine, beef, and small animal focus.
Audubon-Manning Veterinary Clinic
Audubon, IA
Kim Reis Director, Iowa Turkey Federation West Liberty Foods
Ellsworth, IA
Dr Dave Schmitt Acting State Veterinarian for Iowa Iowa Department of Agriculture and Land Stewardship
Des Moines, IA
Marsha Spykerman Sheep producer, Executive Director of the Iowa Sheep Industry Association
Iowa Sheep Industry Association
Sibley, IA
Dr Mahlon "Whitey" Vorhies
Former Director SDSU and KSU VDLs, former Department Chair at KSU
Amana, IA
VDL Faculty List
Halbur, Pat Chair of VDPAM and Executive Director of the ISU VDL
Administration, Circovirus, Hepatitis E Virus, Swine Respiratory Disease, PRRSV
Hoffman, Lorraine Director of Operations, ISU VDL
Administration, Diagnostic Bacteriology, Antimicrobial Susceptibility Testing
Clothier, Kris Adjunct Instructor, Bacteriology
Beef, Cattle Herd Health, FADs, Johne's Disease
Cooper, V.L. Senior Clinician, Pathologist BVD, Beef Cattle, Dairy, Caprine, Equine, Canine, Feline
Ensley, Steve
Clinician, Toxicology Section Leader
Toxicology, Field Investigation of Tox Issues, Drinking Water Quality, Corn co-products
Frana, Tim Associate Professor Bacteriology Section Leader
Bacterial Diseases, Epidemiology, Diagnostics
Gauger, Phil Clinician Pathology, Virology, Swine Influenza, PRRSV, PCV2
Harmon, Karen Adjunct Assistant Professor, Molecular Microbiology
Molecular Testing and Characterization of Animal Bacteria and Viruses
Hyde, Walter Professor and Section Leader, Chemistry, Racing Chemistry, Bioanalytical Services
Toxicology, Chemistry, Analytical Toxicology
Imerman, Paula Adjunct Assistant Professor and Lab Manager
Chemistry/Toxicology [email protected]
Janke, Bruce Professor, Pathology Swine Influenza, Enteric Diseases of Pigs, Pathology
Johnson, John Clinician and Serology Section Leader
Development of Serology Tests and Quality Assurance
Kim, Won-Il Clinician Molecular Diagnostics and Research, PRRSV, SIV, Circovirus
Kinyon, Joann Adjunct Assistant Professor, Bacteriology/Clinical Microbiology Section Leader
Bacteriology/Clinical Microbiology [email protected]
Loynachan, Al Assistant Professor Pathology, Swine and Equine Diseases [email protected] Madson, Darin Adjunct Instructor, Pathology Swine Disease Diagnosis, Nursery/Grow-
Finish Health [email protected]
Opriessnig, Tanja Assistant Professor, Pathologist
Porcine Circovirus, Erysipelas, Hepatitis E virus, Porcine Parvovirus, Swine Respiratory Diseases
Osweiler, Gary Professor, Toxicologist Toxicology, Agricultural Chemicals, Poisonous Plants and Mycotoxins
Patterson, Abby Adjunct Instructor Pathology [email protected] Ramamoorthy, Sheela
Clinician, Serology Immunology, Serology, Vaccinology
Rathje, John Clinician Pathology, Dairy, Swine Diseases [email protected] Schwartz, Kent Clinician, Pathology Section
Leader Pathology, Swine and Beef Diagnostics, Evidence-Based Practice
Strait, Erin Clinician Immunology, Diagnostic Research and Development, Swine Mycoplasma
Yoon Kyoung-Jin Professor and Section Leader for Virology and Molecular Diagnostics
Viral Diseases, PRRSV, Influenza, PCV2, Development of Diagnostic Tools
Zimmerman, Jeffrey Professor, Epidemiologist Ecology of Viral Infections of Swine
7
Faculty Areas of Expertise
8
VDL Staff List Bacteriology
Carter, Sylvia Laboratory Technician II BS [email protected]
Erickson, Barb Research Technician [email protected]
Frisk, David Clerk II [email protected]
Fulster, Susan Laboratory Technician II BS [email protected]
Klinefelter, Timothy Research Associate II BS [email protected]
McDeid, Leona Assistant Scientist I AA, BA [email protected]
Murphy, Dorothy Laboratory Technician III BS [email protected]
Naberhaus, Nadine Research Associate II BS [email protected]
Ouyang, Grace Research Associate I BS [email protected]
Terry, Linda Research Associate I BA [email protected]
Thompson, Curt Assistant Scientist II BS, MBA [email protected]
Zavala, Marissa Research Associate I BS [email protected]
Bioanalytical Systems
Chriswell, Amy Research Associate II BS [email protected]
Kinker, Julia Research Associate II [email protected]
Sickau, Diane Research Associate I BS [email protected]
Chemistry/Toxicology
Ivanytska, Olha Laboratory Technician II [email protected]
Kasper, Lisa Laboratory Technician III BS [email protected]
Moore, Ronda Research Technician BS [email protected]
Schrunk, Dwayne Laboratory Technician III BS [email protected]
Slater, Kathy Laboratory Technician I [email protected]
Clerical
Adams, Carolyn Clerk III [email protected]
Alt, Nancy Clerk IV BA [email protected]
Finch, Mary Clerk III [email protected]
Holdredge, Jennifer Office Coordinator BS [email protected]
Koeneke, Stacy Clerk III [email protected]
Lee, Christine Account Clerk [email protected]
Meraz, Christine Clerk IV [email protected]
Olson, Linda Clerk III [email protected]
Tuttle, Vanna Clerk III [email protected]
Wilson, Kristine Clerk III [email protected]
Zagar, Rose Clerk III BS [email protected]
Histopathology
Branaman, Rosalyn Histology Technician II BS [email protected]
Brodie, Joseph Assistant Scientist I AA, BLS [email protected]
Elsberry, Alan Histo Technician I [email protected]
Fenneman, Dawn Histo Technician I BS [email protected]
Schwarz, Brenda Laboratory Technician I BS [email protected]
Watts, Capri Histology Technician I [email protected]
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Informatics
Boyes, Neil Systems Analyst I BA, BS [email protected]
Garza, Joseph System Analyst I BS [email protected]
Hall, Timothy System Support Specialist II BS [email protected]
Jaya, Arnon Systems Analyst II BS [email protected]
Mindham, Steven Systems Support Specialist III BS [email protected]
Berghefer, Randy Manager Information Technology I
AA, BS [email protected]
Pathology
Boor, Jessica Assistant Scientist I BS [email protected]
Grover, Raymond Jr. Laboratory Technician III BA [email protected]
Jennings, Michael Laboratory Technician II [email protected]
Timmer, Heidi Laboratory Technician II [email protected]
Quality Systems
Meetz, Michael Quality Systems Coordinator BS [email protected]
Middleton, Shawna Quality Systems Manager AA, BS [email protected]
Smith, Linda Clerk I BA [email protected]
Racing Chemistry
Auferonak, Iryna Laboratory Technician II [email protected]
Barchman, Sarah Laboratory Technician I BS [email protected]
Berrett, Kathy Secretary III [email protected]
Chang, Jenny Laboratory Technician II BS, MS [email protected]
Dobson, Lori Laboratory Technician III BS [email protected]
Endreland, Luanne Laboratory Technician II [email protected]
Gilloon, Daniel Laboratory Technician I BS [email protected]
Greulich, David Research Associate I [email protected]
Kind, Albert Program Manager I BA [email protected]
Morgan, Martha Research Associate I BA, EMT-P [email protected]
Novak, Renee Research Associate I AA [email protected]
Peterson, Jacqueline Research Associate I BA [email protected]
Rahfeldt, Angie Research Associate I BS [email protected]
Rice, Somchai Laboratory Technician III BS [email protected]
Schultz, Daniel Laboratory Technician III BS [email protected]
Sieve, Kristal Laboratory Technician III BS [email protected]
Stewart, Jason Laboratory Technician II [email protected]
Wulf, Larry Laboratory Technician II BS, MS, PhD [email protected]
Serology
Block, Susanne Laboratory Technician II BS [email protected]
Boesenberg-Smith, Kelly
Assistant Scientist II BS, MS [email protected]
Clauson, Susan Clerk III [email protected]
Dunn, Linda Clerk III [email protected]
Emge, Patrick Laboratory Technician II BS [email protected]
Fowles, Beverly Laboratory Technician III [email protected]
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Gard, Theresa Laboratory Technician II BS [email protected]
Heinen, Sheila Laboratory Technician III BS [email protected]
Jarboe, Charlene Laboratory Technician II [email protected]
Johnson, Alison Research Associate I [email protected]
Kalkwarf, Erin Research Associate I BS [email protected]
Lin, Kathy Research Associate II BS [email protected]
Magtoto, Ronaldo Research Associate I BS, MS, PhD [email protected]
Nelson, Nancy Laboratory Technician II AA, BS [email protected]
Norris, Sheila Assistant Scientist II BS [email protected]
Schuller, Bruce Laboratory Assistant II [email protected]
Upchurch, Nancy Laboratory Technician II [email protected]
Williams, Audrey Clerk III BA [email protected]
Virology/Molecular Diag
Abate, Sarah Research Associate I [email protected]
Allam, Ahmad Research Associate I [email protected]
Chittick, Wayne Associate Scientist BS [email protected]
Eshelman, Raquel Research Associate I BS [email protected]
Haney, Devon Research Associate I BS [email protected]
Kostohryz, Scott Research Associate I BS [email protected]
Murken, Cheryl Clerk III AA [email protected]
Stensland, Wendy Research Associate II, Lab Manager
Yager, Kathryn Research Associate I BS [email protected]
Zhou, Fanghong Research Associate I DVM [email protected]
Research
Jobgen, Scott Research Associate I BS [email protected]
Shen, Huigang Post Doc PhD [email protected]
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Surveillance Testing
2008 Number of tests
Total 202,647
AIV PCR - Wildlife, backyard, and game birds 1,197
AIV Serology 23,140
Brucella serology/Feral Swine 937
BSE 61
CSF PCR 443
Johne’s fecal culture 161
Johne’s serology 1,224
Johne’s PCR 66
Meat juice – PRRSV serology 96,770
Meat juice – PRV serology 65,870
PRV tissue FA 260
PRV Serum Random Sampling 9,368
Salmonella enteritidis (poultry) 3,150
New Faculty Hired
Name Position Section Degrees
Frana, Tim Associate Professor Bacteriology DVM, MS, PhD, ACVPM & ACVM Board Certified
Gauger, Phil Clinician Pathology DVM
Ramamoorthy, Sheela Clinician Serology DVM, MS, PhD
Rathje, John Clinician Pathology DVM
Strait, Erin Clinician Virology/Molecular Diagnostics
DVM, PhD
Stevenson, Greg Professor Pathology DVM
New Staff Hired
Name Position Section
Abate, Sarah Research Associate I Virology/Molecular Diag
Allam, Ahmad Research Associate I Virology/Molecular Diag
Barchman, Sarah Laboratory Technician I Racing Chemistry
Bower, Leslie Research Associate I Molecular Diagnostics
Chittick, Wayne Associate Scientist Virology/Molecular Diag
Erickson, Barb Research Technician Bacteriology
Garza, Joseph System Analyst I Informatics
Jobgen, Scott Research Associate I Opriessnig
Johnson, Alison Research Associate I Serology
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Koeneke, Stacy Clerk II Clerical
Murken, Cheryl Clerk III Virology/Molecular Diag
Osorio, Sue Research Associate I Virology/Molecular Diag
Shen, Huigang Post Doc Opriessnig
Slater, Kathy Laboratory Technician I Chemistry/Toxicology
Spitz, Allison Laboratory Technician I Chemistry/Toxicology
Timmer, Heidi Laboratory Technician II Pathology
Upchurch, Nancy Laboratory Technician II Serology
Zavala, Marissa Research Associate I Bacteriology
New Tests Introduced
Name Test Section
Brachyspira hyodysenteriae or B.pilosicoli PCR Molecular Diagnostics
Calf diarrhea panel PCR Molecular Diagnostics
Mycoplasma Tween 20 Serology
PCV2 - 4 dilution IFA Serology
PCV2 differential ELISA Serology
Porcine Mycoplasma Culture Bacteriology
SIV H1N1 (31) - Pfizer strain HI Serology
SIV H1N2 – human-like HI Serology
Tritrichomonas foetus PCR Molecular Diagnostics
13
2008 Statistical Data 2008 Number of Accessions Number of Tests
Total 41,957 1,157,388
Jan 3,576 100,710
Feb 3,157 85,476
Mar 3,373 83,745
Apr 3,877 161,828
May 3,740 147,517
Jun 3,074 76,306
Jul 3,797 75,962
Aug 3,538 94,841
Sep 3,628 101,045
Oct 3,913 79,572
Nov 3,051 78,916
Dec 3,233 71,470
2008 Accessions Bacteriology
Bioanalytical Services
Chemistry / Toxicology
Molecular Diagnostics Pathology Serology Virology
Total 13,859 3,094 889 13,463 12,628 17,806 3,864
Jan 1,301 276 79 1,154 1,198 1,440 360
Feb 1,142 285 54 1,076 1,003 1,289 388
Mar 1,251 203 96 1,087 1,124 1,382 284
Apr 1,331 331 80 1,172 1,251 1,685 349
May 1,216 194 81 959 1,136 1,741 412
Jun 923 295 79 839 880 1,311 278
Jul 1,106 313 84 1,176 999 1,684 256
Aug 1,100 128 71 1,137 971 1,542 338
Sep 1,181 294 70 1,250 987 1,557 306
Oct 1,291 261 80 1,363 1,188 1,657 336
Nov 961 187 54 1,052 924 1,303 343
Dec 1,056 327 61 1,198 967 1,215 214
2008 Tests Bacteriology Bioanalytical
Services Chemistry / Toxicology
Molecular Diagnostics Pathology Serology Virology
Total 111,772 20,573 2,413 73,625 107,941 831,745 9,319
Jan 10,120 1,825 243 6,781 10,212 70,749 780
Feb 8,868 1,640 158 5,758 6,607 61,744 701
Mar 9,712 1,318 222 5,554 8,215 57,984 740
Apr 12,319 2,139 258 6,302 9,117 130,703 990
May 10,434 1,201 217 4,902 10,169 119,528 1,066
Jun 8,895 1,813 239 4,697 9,849 49,974 839
Jul 7,581 1,914 214 4,673 8,984 51,953 643
Aug 8,785 902 187 5,867 7,395 71,047 658
Sep 9,758 2,076 174 5,865 8,462 74,043 667
Oct 9,171 1,946 185 7,621 8,283 51,696 670
Nov 8,366 1,462 192 4,833 12,392 50,999 672
Dec 7,764 2,337 124 5,978 8,256 41,325 893
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Accessions by Species Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec Total
Grand Total 3576 3157 3373 3877 3740 3074 3797 3538 3628 3913 3051 3233 41,957
Porcine 2114 1925 1859 1960 1932 1624 2075 2036 2055 2272 1770 1953 23,575
Bovine 669 545 633 888 801 512 602 536 518 641 585 549 7,479
Canine 312 296 306 301 270 322 317 287 311 378 296 298 3,694
Avian 192 150 154 223 203 147 297 145 272 233 140 168 2,324
Equine 91 94 239 289 333 245 247 227 200 145 72 53 2,235
Feline 61 55 61 75 71 75 107 85 86 86 70 81 913
Bat 16 14 9 14 12 32 10 91 47 12 12 24 293
Non-Animal 19 17 22 10 19 31 21 24 25 41 23 16 268
Caprine 23 8 20 32 21 11 24 34 28 16 14 10 241
Unknown 18 7 9 24 28 22 26 18 20 11 13 24 220
Ovine 22 20 20 23 14 23 22 13 8 12 8 12 197
Cervidae 7 7 10 4 11 3 11 4 23 23 6 13 122
Camelid 6 5 11 10 3 8 14 16 16 18 8 6 121
Rodent 7 3 3 7 6 6 6 3 5 6 8 13 73
Procyonidae 2 3 8 5 3 7 8 4 7 6 8 2 63
Lagomorpha 5 2 3 4 1 2 4 5 3 29
Mustelid 1 1 1 2 2 3 1 2 1 3 2 3 22
Marsupial 2 2 2 1 2 4 1 2 1 2 19
Reptilian 4 1 1 1 2 1 1 11
Fish 1 2 2 1 1 3 10 Canine Exotic 1 1 2 1 1 2 8
Primate 2 1 1 1 1 1 1 8
Antelope 2 3 5 Aquatic Mammal 1 1 1 1 1 5
Snake 1 2 2 5
Amphibian 1 1 1 1 4
Bison 3 1 4
Insectivora 1 1
Other 1 8
Porcine 57%
Bovine 18%
Canine 9%
Avian 6%
Equine 5%
Feline 2%
Bat 1%
Non-Animal 1%
Caprine 1%
Percentage of Accessions by Species
Porcine
Bovine
Canine
Avian
Equine
Feline
Bat
Non-Animal
Caprine
15
Accessions by Species/Section Bacteriology
Bioanalytical Services
Chemistry / Toxicology
Molecular Diagnostics Pathology Serology Virology
Porcine 7,948 3,094 232 11,700 7,662 10,630 1,302
Bovine 2,310 - 320 1,250 2,980 3,094 1,291
Canine 1,691 - 67 35 1,029 763 362
Avian 532 - 25 270 284 1,354 27
Equine 382 - 26 29 128 1,718 51
Feline 403 - 11 2 206 3 360
Caprine 116 - 24 12 97 85 7
Non-Animal 141 - 128 26 - - 25
Bat 1 - 1 - 23 - 291
Ovine 103 - 29 13 99 31 7
Unknown 125 - 24 4 11 1 -
Cervidae 31 - 5 3 25 76 1
Camelid 5 - 1 88 5 31 9
Rodent 11 - - 24 14 - 32
Procyonidae 2 - - 2 3 - 62
Lagomorpha 10 - 5 - 11 9 4
Mustelid 5 - 1 3 10 1 14
Marsupial 8 - - 1 5 1 10
Fish 6 - - - 10 - -
Reptilian 7 - - - 4 - -
Canine Exotic 1 - - - 4 - 5
Primate 4 - - - 1 3 -
Amphibian 3 - - - 3 -
Antelope 1 - - - 1 4 -
Exotic 2 - - - 3 - 1
Snake 1 - - - 5 - -
Aquatic Mammal 4 - - - 1 - -
Bison 3 - - - 1 1 -
Porcine Exotic 1 - - 1 1 - 1
Bovine Exotic 1 - - 1 1 1
Ovine Exotic - - 1 - 1 - -
Feline Exotic 1 - - - - - -
Insectivora - - - - - - 1
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Statistical Trends
Totals Accessions Tests
2008 41,957 1,157,388
2007 47,957 1,039,032
2006 47,238 1,330,084
2005 38,907 1,005,301
2004 35,161 911,889
2003 40,367 1,063,336
Bacteriology Bioanalytical
Services Chemistry / Toxicology
Molecular Diagnostics Pathology Serology Virology &
Accessions
2008 13,859 3,094 889 13,463 12,628 17,806 3,864
2007 16,366 3,780 1,117 17,110 15,582 20,327 9,982
2006 16,110 3,635 1,357 ** 15,422 20,524 16,608
2005 12,924 1,168 1,823 ** 12,153 17,661 11,929
2004 11,191 * 645 ** 10,832 17,619 9,090
Tests
2008 111,772 20,573 2,413 73,625 107,941 831,745 9,319
2007 102,899 25,539 3,051 64,223 124,094 707,628 9,000
2006 86,088 20,314 2,123 ** 98,922 984,335 74,681
2005 72,732 6,621 1,934 ** 91,476 691,573 42,269
2004 71,429 * 1,861 ** 82,649 643,077 24,352 *Bioanalytical Services Section formed in 2005 ** Molecular testing combined with Virology
Top 10 Species 2004 2005 2006 2007 2008
Grand Total of Cases 35,161 38,907 47,238 47,957 41,957
Porcine 17,732 20,912 28,109 27,847 23,575
Bovine 6,827 7,145 7,384 8,275 7,479
Canine 3,537 3,762 3,677 3,794 3,694
Avian 1,550 1,674 2,563 2,750 2,324
Equine 3,302 3,012 2,833 2,646 2,235
Feline 949 1,081 960 984 913
Bat 319 276 269 265 293
Ovine 205 206 223 239 197
Caprine 101 182 223 238 241
Non-Animal 81 114 181 198 268
17
Laboratory Section Overviews
Bacteriology
Percent of Accessions by species
Bacteriology 5- year trend 2004 2005 2006 2007 2008
Total number of tests 71,429 72,732 86,088 102,899 111,772
Basic tests
Bacterial ID Extended 693 1,119 1,453 1,497 4,910
Bacterial ID Short 5,689 6,008 7,059 14,089 14,814
Bacterial identification from mailed culture 51 81 77 272 1,109
Blood Culture 27 28 45 51 35
Brachyspira Culture 1,737 1,203 1267 1,442 3,139
Campylobacter and Trichomonas 958 1,162 913 935 1,582
Campylobacter Culture 428 356 407 420 506
Clin Micro Culture - Aerobic and Anaerobic 995 1,187 1,206 1,329 1,339
Clostridium difficile culture 9 34 44 76 74
Clostridium FA 28 30 21 30 36
Coliform Test (Membrane Filter) 3 7 7 328 498
Culture Summary 473 495 427 485 529
Direct Smear 106 140 123 82 68
E. coli K99 LA Typing 343 373 386 502 394
Egg Culture 72 112 133 216 156
Enrichments 988 1822 599 702 903
Fungal Culture Results 267 353 281 263 341
Identification System 33 67 72 382 1,081
Johne's Culture 377 532 545 66 -
Johne's Liquid Culture - - 182 849 965
Leptospira FA 449 454 498 108 26
57%17%
12%
4%
3% 3% 1%
1%
0%1%
1% 0% 0%
0%
0%
Bacteriology
Porcine
Bovine
Canine
Avian
Equine
Feline
Caprine
Non-Animal
18
Bacteriology 5- year trend 2004 2005 2006 2007 2008
Milk Culture 525 429 348 658 264
Milk Culture - Basic - - - 886 6,395
Milk Culture - Basic with Mycoplasma - - - 1,319 4,949
Milk Culture – Bulk Tank 19 24 34 74 122
Mycoplasma Culture 409 275 1,669 318 347
Mycoplasma Id 95 60 95 25 2
Mycoplasma Milk Culture - - - 3 -
Porcine Mycoplasma Culture - - - - 266
Poultry - Salmonella Environmental 2,693 2,805 143 3,137 3,150
Routine Culture 39,921 37,588 49,360 49,741 41,378
Routine Culture - Aerobic and Anaerobic 984 1,465 1,859 1,755 2,220
Salmonella Id 1,024 914 1,392 1,539 1,364
Serogrouping Lancefield & Salmonella 56 46 25 23 48
Serotype – Actinobacillus pleuropneumoniae 164 158 196 179 139
Serotype - Streptococcus suis 70 42 46 69 52
Special Culture (Nasal, Feces, Semen, Other) 967 608 281 179 757
Standard Plate Count Results 104 34 48 450 152
Tetra enrichment - - - 7,475 7,254
Trichomonas Culture Results - - - 294 171
Susceptibility testing
Bovine Mastitis Kirby Bauer 34 20 11 27 30
Bovine Respiratory Kirby Bauer 22 33 26 24 19
Companion/Equine MIC 108 136 98 199 262
Equine Kirby Bauer 155 211 210 209 233
E-TEST 52 83 73 66 56
Food Animal MIC 6,136 6,484 7,627 8,324 7,879
Mastitis MIC 150 191 138 108 76
Multiresistant Kirby Bauer 64 54 86 125 61
Porcine Kirby Bauer 17 11 51 15 6
Poultry Susceptibility 109 128 86 115 102
Small Animal Kirby Bauer 814 830 870 890 775
Special Tests
Antinuclear Antibody 16 12 19 5 2
Culture of feed for Clostridium perfringens 1 2 - 3 2
agELISA – Chlamydia 47 68 55 52 110
ELISA – Clostridium difficile toxin A/B 271 267 264 459 495
ELISA – Occult Heartworm Antibody 2 - - - -
ELISA – Occult Heartworm Antigen 103 - - - -
ELISA – Parvo Virus 11 11 3 4 4
Rheumatoid Factor Agglutination 2 1 4 1 1
SRID for IgG 30 55 4 25 124
19
Bovine Porcine
2008 Salmonella serotypes (# of isolates) Group 2005 2006 2007 2008 2005 2006 2007 2008
Salmonella typhimurium (copenhagen) B 13 5 15 9 179 178 299 247
Salmonella derby B 4 4 4 5 72 145 160 144
Salmonella typhimurium B 7 10 8 10 34 212 125 121
Salmonella agona B 4 5 7 6 33 46 84 71
Salmonella heidelberg B 2 4 - 3 43 77 71 62
Salmonella infantis C1 - 4 1 1 28 35 40 39
Salmonella anatum E 6 5 7 5 16 35 33 30
Salmonella senftenberg E 1 - 3 - 19 24 26 28
Salmonella johannesburg B 1 2 - - 11 17 38 27
Salmonella worthington - 2 2 - 9 23 21 26
Salmonella choleraesuis (kunsendorf) C1 - - - - 8 13 18 21
Salmonella ohio C1 - 2 - - 6 10 17 16
Salmonella mbandaka C1 2 4 3 1 5 9 12 15
Salmonella schwarzengrund B - - 4 4 6 7 11 15
Salmonella brandenburg B - - - - 5 11 4 14
Salmonella london E 1 2 7 4 2 9 8 12
Salmonella muenchen C2 - - - 1 2 14 18 11
Salmonella 6,7:nonmotile - - - - 2 6 13 8
Salmonella uganda E 13 9 17 9 8 8 2 8
Salmonella bovismorbificans C2 2 - - 1 5 3 2 7
Salmonella newport C2 27 16 13 7 6 11 14 6
Salmonella muenster E 11 15 5 7 1 2 2 1
Salmonella dublin D 20 52 69 41 1 2 2 1
Bacteriology 2008 APP serotypes Porcine
Untypeable 52
Type 7 41
Type 5 28
Type 1 9
Type 12 2
Type 3 5
20
Antimicrobial Susceptibility Profiles
Note: The susceptibility information presented below is a summary of data gathered at ISU VDL for the time period listed. Interpretation and application of this information to a given case or trend may lead to false conclusions.
Avian Susceptibility Profile of Avian Pathogens Submitted to ISU VDL in 2007-2008
E coli G ana P mult Salm Salm B Salm C1
Salm C2
# tested 143 4 8 15 13 11 5
Percent susceptible***
Amoxicillin 64% 75% 100% 60% 69% 91% 60%
Ceftiofur 94% 100% 100% 87% 85% 91% 80%
Enrofloxacin 99% 100% 100% 100% 100% 100% 100%
Florfenicol 34% 100% 100% 40% 54% 27% 80%
Neomycin 84% 50% 100% 93% 92% 100% 100%
Oxytetracycline 29% 0% 75% 73% 62% 82% 60%
Streptomycin 51% 100% 38% 53% 46% 91% 20%
Sulfadimethoxine 41% 0% 13% 40% 38% 73% 40%
Sulphathiazole 49% 0% 38% 67% 46% 91% 60%
Tetracycline 28% 0% 75% 73% 62% 82% 60% Trimethoprim/Sulphamethoxazole 92% 100% 88% 100% 100% 100% 100%
Bovine MIC Susceptibility Profile of Bovine Pathogens Submitted to ISU VDL in 2008
E coli
99 neg E coli 99 + H som M bov M hem P mult P tre S dubl S new S typm S ugan
# tested 480 15 37 10 122 83 2 37 7 19 9
Percent susceptible***
Ampicillin 30% 73% 97% 100% 82% 98% 100% 11% 43% 16% 22%
Ceftiofur** 59% 87% 100% 100% 100% 100% 100% 16% 43% 47% 22%
Cephalothin 60% 73% ND 100% 100% 100% ND ND ND ND ND
Chlortetracycline 11% ND 97% 100% 78% 94% 100% 3% 29% 16% 11%
Clindamycin 0% 0% 57% 20% 0% 0% 0% 0% 0% 0% 0%
Danofloxacin** 65% ND 65% 0% 80% 81% 100% ND ND ND ND
Enrofloxacin** 70% ND 65% 100% 82% 85% 100% 100% 100% 100% 100%
Erythromycin 0% 0% ND 100% 0% 67% ND 0% 0% 0% 0%
Florfenicol** 9% ND 92% 100% 87% 86% 100% 8% 14% 5% 0%
Gentamicin 58% 0% 32% 100% 84% 73% 100% 97% 86% 84% 11%
Neomycin 23% 0% 5% 100% 55% 41% 100% 51% 71% 79% 33%
Novobiocin 0% 0% ND ND ND 100% ND ND ND ND ND
Oxacillin ND ND ND ND ND ND ND ND ND ND ND
Oxytetracycline** 8% ND 43% 100% 48% 63% 50% 3% 29% 16% 11%
Penicillin 0% 0% 95% 10% 22% 73% 50% 0% 0% 0% 0% Penicillin / Novobiocin 0% 0% ND ND ND 100% ND ND ND ND ND
Spectinomycin** 0% ND 59% 89% 76% 73% 0% 0% 0% 0% 0%
Sulfachloropyridazine 30% 60% ND 100% 100% 100% ND 0% 0% 0% 0%
Sulfadiazine 80% 80% ND ND ND 0% ND ND ND ND ND
Sulfadimethoxine 12% 60% 35% 89% 21% 20% 50% 3% 0% 0% 0%
Tetracycline 50% 67% ND 100% 100% 100% ND ND ND ND ND
Tiamulin 0% ND 100% 100% 49% 59% 100% 3% 0% 0% 0%
Tilmicosin** 0% ND 84% 90% 66% 69% 100% 0% 0% 0% 0% Trimethoprim / Sulphamethoxazole 42% 0% 100% 100% 3% 5% 100% 76% 71% 89% 89%
Tulathromycin** 0% ND 78% 0% 81% 83% 0% ND ND ND ND
Tylosin (Tartrate/Base) 0% ND 65% 0% 0% 0% 0% 0% 0% 0% 0%
21
Bovine Mastitis MIC Susceptibility Profile of Bovine Mastitis Pathogens Submitted to ISU VDL in 2007 - 2008
E coli Ente K
pneu P mult S aur S dysg S epi S ube
# tested 27 6 4 4 66 28 10 35
Percent Susceptible
Ampicillin 78% 67% 0% 100% 76% 96% 70% 97%
Ceftiofur** 85% 67% 75% 100% 82% 82% 60% 80%
Cephalothin 78% 17% 100% 100% 98% 100% 90% 97%
Erythromycin 0% 0% 0% 25% 85% 86% 90% 74%
Novobiocin 0% 0% 0% 100% 89% 75% 67% 33%
Oxacillin ND ND ND ND 94% ND ND ND
Penicillin 0% 0% 0% 100% 76% 93% 60% 71% Penicillin / Novobiocin** 0% 0% 0% 100% 97% 96% 90% 100%
Pirlimycin** 0% 0% 0% 0% 82% 93% 80% 71%
Sulfachloropyridazine 75% 100% 100% 100% 100% 75% 100% 0%
Sulfadiazine 75% 100% 100% 0% 33% 25% 33% 0%
Sulfadimethoxine 53% 0% 50% 50% 75% 71% 86% 3%
Tetracycline 67% 67% 50% 75% 89% 43% 70% 46%
Canine Ear Canal KB Susceptibility Profile of Ear Canal Pathogens Received at ISU VDL in 2007 - 2008
E coli Enc P aer Prot Pseu S aur S can S equs S int S schl
# tested 22 13 73 32 6 5 24 17 43 15
Percent Susceptible***
Amikacin 95% 23% 86% 100% 83% 100% 4% 6% 100% 100%
Amoxicillin/Clav 50% 100% 3% 94% 0% 100% 100% 94% 86% 87%
Cefpodoxime 86% 38% 1% 94% 0% 100% 100% 94% 86% 87%
Cephalothin 18% 8% 3% 88% 0% 100% 100% 88% 86% 87%
Ciprofloxacin 77% 46% 84% 100% 67% 100% 79% 71% 93% 67%
Clindamycin** 0% 15% 1% 0% 0% 100% 58% 71% 88% 93%
Enrofloxacin** 77% 31% 23% 97% 33% 100% 75% 65% 91% 60%
Gentamicin 77% 69% 84% 97% 83% 100% 50% 41% 91% 87%
Imipenem 100% 100% 89% 91% 50% 100% 92% 100% 91% 93%
Marbofloxacin 77% 31% 67% 100% 50% 100% 83% 76% 91% 67%
Neomycin 86% 31% 33% 91% 33% 100% 4% 0% 88% 100%
Orbifloxacin** 77% 15% 18% 94% 17% 100% 42% 29% 91% 60%
Polymixin B 100% 8% 96% 13% 50% 100% 8% 18% 95% 100%
Tetracycline 77% 62% 0% 13% 17% 60% 29% 29% 74% 100%
Ticarcillin/Clav 68% 54% 88% 100% 33% 100% 100% 100% 88% 87%
Ticarcillin 68% 38% 86% 97% 33% 100% 100% 100% 88% 87%
Tobramycin 77% 38% 93% 97% 50% 100% 17% 6% 91% 100%
Trimeth/Sulfadiazine 82% 92% 4% 97% 33% 100% 96% 94% 84% 100%
Oxacillin ND ND ND ND ND 100% ND ND 81% 73%
Vancomycin 0% 0% 0% 3% 0% 0% 0% 0% 2% 0%
22
Canine Disk Diffusion Susceptibility Profile of Canine Pathogens Submitted to ISU VDL in 2007 - 2008
B bron E coli E fael E faem Ente K pneu Paer P mult Pseu S aur S can S int
# tested 43 432 77 35 42 31 74 20 36 41 53 235
Percent Susceptible***
Amikacin 100% 95% 5% 11% 95% 94% 97% 100% 69% 100% 4% 99% Amoxicillin / Clavulanic Acid 100% 63% 97% 40% 17% 55% 3% 100% 44% 88% 100% 85%
Ampicillin** 23% 37% 97% 40% 17% 3% 0% 100% 28% 24% 96% 25%
Azithromycin 100% 18% 13% 7% 20% 0% 0% ND 0% 0% 0% 5%
Carbenicillin 100% 3% 63% 7% 20% 0% 32% ND 25% 0% 100% 21%
Cefazolin 0% 73% 0% 17% 14% 75% 0% 100% 25% 100% 100% 97%
Cefoxitin 4% 72% 0% 0% 29% 75% 0% 100% 25% 100% 100% 97%
Cefpodoxime 4% 75% 0% 0% 71% 75% 0% 100% 0% 100% 100% 97%
Ceftazidime 100% 30% 13% 0% 40% 100% 100% ND 100% 0% 100% 16%
Ceftiofur 5% 79% 56% 9% 74% 74% 3% 95% 33% 88% 100% 81%
Cephalothin 44% 36% 5% 14% 12% 52% 0% 100% 22% 85% 100% 85%
Chloramphenicol 51% 21% 10% 23% 17% 16% 0% 15% 3% 12% 5% 15%
Ciprofloxacin 100% 3% 0% 0% 20% 0% 92% ND 75% 0% 100% 11%
Clindamycin** 0% 0% 0% 11% 0% 0% 0% 15% 8% 83% 54% 74%
Doxycycline 100% 3% 63% 13% 0% 0% 0% ND 25% 50% 100% 74%
Enrofloxacin** 93% 80% 29% 11% 76% 84% 28% 100% 53% 95% 66% 86%
Erythromycin 16% 0% 10% 23% 0% 0% 1% 70% 25% 76% 88% 73%
Gentamicin** 98% 83% 62% 26% 83% 90% 92% 100% 67% 88% 45% 90%
Imipenem 100% 99% 100% 19% 100% 100% 100% 100% 88% 63% 100% 72%
Kanamycin 100% 55% 25% 7% 40% 0% 4% ND 50% 0% 0% 5%
Marbofloxacin 0% 81% 0% 0% 71% 75% 0% 0% 0% 100% 100% 97%
Neomycin 95% 82% 13% 10% 83% 78% 34% 100% 66% 86% 2% 77%
Orbifloxacin** 88% 78% 1% 6% 64% 84% 12% 100% 47% 95% 21% 87% Ormetoprim / Sulfadimethoxine 0% 1% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0%
Oxacillin ND ND ND ND ND ND ND ND ND 83% ND 81%
Penicillin 0% 0% 90% 23% 0% 0% 1% 95% 11% 24% 100% 22%
Piperacillin 100% 3% 100% 13% 20% 0% 100% ND 100% 0% 100% 5%
Polymixin B 100% 100% 0% 0% 80% 100% 96% ND 75% 50% 0% 95%
Rifampin 25% 1% 50% 83% 0% 0% 0% 100% 50% 100% 100% 94%
Sulfachloropyridazine 11% 77% 8% 0% 74% 70% 11% 100% 53% 66% 75% 71%
Tetracycline 93% 68% 58% 31% 55% 65% 7% 100% 50% 68% 16% 63%
Ticarcillin 54% 42% 21% 10% 33% 17% 89% 100% 50% 75% 100% 69% Ticarcillin / Clavulanic Acid 96% 56% 50% 10% 42% 50% 84% 100% 50% 75% 100% 70%
Tobramycin 100% 58% 50% 0% 80% 0% 100% ND 100% 50% 0% 47% Trimethoprim / Sulphamethoxazole 58% 76% 96% 40% 86% 81% 9% 95% 42% 78% 100% 75%
23
Multiresistant - canine & feline Susceptibility Profile of Canine/Feline Pathogens Received at ISU VDL in 2007 - 2008
Abua E coli E fael E faem Ente P aer Pseu S int
# tested 10 33 8 17 5 37 4 20
Percent Susceptible***
Azithromycin 10% 18% 13% 6% 20% 3% 0% 10%
Carbenicillin 0% 3% 63% 6% 20% 27% 25% 20%
Ceftazidime 0% 30% 13% 0% 40% 100% 100% 20%
Ciprofloxacin 0% 3% 0% 0% 20% 92% 75% 15%
Doxycycline 0% 3% 63% 18% 0% 0% 25% 75%
Imipenem 100% 94% 100% 12% 100% 92% 100% 30%
Kanamycin 80% 55% 25% 12% 40% 3% 50% 5%
Piperacillin 0% 3% 100% 12% 20% 100% 100% 5%
Polymixin B 90% 100% 0% 0% 80% 97% 75% 95%
Ticarcillin 30% 3% 25% 6% 0% 78% 25% 20%
Caprine / Ovine Susceptibility Profile of Caprine/Ovine Pathogens Received at ISU VDL in 2007 - 2008
Ecoli 99 - M haem P mult P tre
# tested 58 39 18 9
Percent Susceptible***
Ampicillin 59% 97% 100% 100%
Ceftiofur 86% 100% 100% 100%
Chlortetracycline 23% 95% 100% 33%
Danofloxacin 70% 100% 94% 89%
Enrofloxacin 88% 100% 100% 100%
Florfenicol 16% 100% 100% 100%
Gentamicin 86% 100% 94% 100%
Neomycin 64% 92% 100% 89%
Oxytetracycline 21% 92% 94% 33%
Penicillin 0% 10% 78% 0%
Spectinomycin 2% 95% 94% 11%
Sulfachloropyridazine 100% 100% ND ND
Sulfadiazine 100% ND ND ND
Sulfadimethoxine 32% 69% 22% 89%
Sulphathiazole ND 100% ND ND
Tetracycline 50% ND ND ND
Tiamulin 0% 59% 39% 33%
Tilmicosin 0% 82% 100% 100% Trimethoprim/Sulphamethoxazole 69% 3% 0% 89%
Tulathromycin 0% 24% 39% 0%
24
Equine Disk Diffusiuon Susceptibility Profile of Equine Pathogens Submitted to the ISU VDL in 2007 - 2008
A equ B bron Paer Past P cab R equ S aur S equi S
equs S zoo
# tested 13 6 21 3 2 29 15 27 12 110
Percent Susceptible***
Amikacin 38% 67% 86% 100% 100% 97% 93% 7% 8% 2% Amoxicillin / Clavulanic Acid ND 100% 0% ND ND 50% 75% 100% 100% 100%
Ampicillin** 100% 0% 0% 100% 100% 0% 67% 100% 92% 98%
Cefazolin ND 0% 0% ND ND 0% 75% 100% 100% 100%
Cefoxitin ND 0% 0% ND ND 50% 75% 100% 100% 100%
Cefpodoxime ND 0% 0% ND ND 0% 75% 100% 100% 100%
Ceftazidime ND ND 100% ND ND ND ND ND ND ND
Ceftiofur** 100% 0% 0% 100% 100% 66% 73% 100% 100% 99%
Cephalothin 100% 17% 0% 100% 100% 0% 73% 100% 100% 99%
Chloramphenicol ND 100% 0% ND ND 0% 100% 100% 50% 89%
Ciprofloxacin ND ND 100% ND ND ND ND ND ND ND
Clindamycin 0% 0% 0% 67% 0% 0% 87% 26% 67% 26%
Doxycycline ND ND 0% ND ND ND ND ND ND ND
Enrofloxacin 100% 83% 38% 100% 100% 59% 93% 93% 75% 62%
Erythromycin 31% 0% 0% 67% 100% 90% 80% 96% 83% 91%
Gentamicin** 69% 83% 86% 100% 100% 100% 87% 67% 67% 44%
Imipenem ND 100% 100% ND ND 100% 50% 100% 100% 100%
Marbofloxacin ND 0% 0% ND ND 0% 100% 100% 100% 88%
Neomycin 38% 100% 29% 100% 100% 96% 100% 4% 0% 2%
Orbifloxacin ND 0% 0% ND ND 0% 75% 0% 100% 22%
Oxacillin**** ND ND ND ND ND ND 60% ND ND ND
Penicillin 46% 0% 0% 67% 50% 0% 67% 100% 100% 97%
Polymixin B ND ND 100% ND ND ND ND ND ND ND
Rifampin 62% 0% 0% 67% 100% 93% 87% 100% 100% 94%
Sulfachloropyridazine 85% 50% 0% 100% 100% 74% 82% 77% 82% 55%
Tetracycline 92% 83% 5% 100% 100% 97% 80% 96% 42% 35%
Ticarcillin 100% 67% 73% 100% 100% 0% 73% 100% 100% 99% Ticarcillin / Clavulanic Acid 100% 83% 59% 100% 100% 7% 67% 100% 100% 99% Trimethoprim / Sulphamethoxazole 100% 33% 5% 100% 100% 52% 100% 96% ND 92%
E-test E-test susceptibility for pathogens submitted to the ISU VDL 2007-2008
Amy Bact C diff C perf Clos Fus Pec Pes Prp
# tested 12 58 7 7 3 21 3 8 2
Percent Susceptible***
Cefoxitin 100% 91% 0% 100% 100% 95% 100% 88% 100%
Clindamycin 100% 84% 100% 71% 100% 100% 67% 88% 50%
Metronidazole 75% 98% 100% 100% 100% 90% 100% 100% 50%
Penicillin 100% 59% 100% 86% 100% 95% 67% 88% 100%
25
Feline 2007 - 2008
B bron E coli E fael E faem Ente K pneu P aer P mult Pseu S aur S can S int
# tested 8 55 7 4 5 4 32 24 9 5 8 16
Percent Susceptible***
Amikacin 100% 100% 14% 0% 100% 100% 97% 100% 78% 100% 38% 100% Amoxicillin / Clavulanic Acid 100% 87% 100% 50% 20% 75% 0% 100% 67% 100% 100% 69%
Ampicillin 13% 67% 100% 50% 0% 0% 0% 100% 44% 60% 88% 31%
Azithromycin ND ND ND 0% ND ND 8% ND ND ND ND 100%
Carbenicillin ND ND ND 0% ND ND 17% ND ND ND ND 0%
Cefazolin 0% 92% 0% 0% ND 100% 0% 100% 0% ND 100% 100%
Cefoxitin 0% 92% 0% 0% ND 100% 0% 100% 0% ND 100% 100%
Cefpodoxime 0% 100% 0% 0% ND 100% 0% 100% 0% ND 100% 100%
Ceftazidime ND ND ND 0% ND ND 100% ND ND ND ND 100%
Ceftiofur 13% 93% 29% 0% 80% 100% 0% 100% 56% 100% 100% 69%
Cephalothin 25% 55% 0% 0% 20% 50% 0% 100% 33% 100% 100% 69%
Chloramphenicol 38% 24% 14% 25% 0% 25% 0% 13% 11% 0% 38% 13%
Ciprofloxacin ND ND ND 0% ND ND 92% ND ND ND ND 100%
Clindamycin 0% 0% 0% 0% 0% 0% 0% 4% 11% 100% 38% 81%
Doxycycline ND ND ND 50% ND ND 0% ND ND ND ND 100%
Enrofloxacin 100% 93% 0% 0% 60% 100% 28% 100% 89% 100% 75% 88%
Erythromycin 0% 0% 29% 25% 0% 0% 0% 50% 33% 100% 63% 69%
Gentamicin 100% 95% 71% 50% 80% 100% 91% 100% 78% 100% 50% 94%
Imipenem 100% 100% 100% 33% ND 100% 82% 100% 0% ND 100% 100%
Kanamycin ND ND ND 50% ND ND 0% ND ND ND ND 0%
Marbofloxacin 0% 100% 0% 0% ND 100% 0% 0% 0% ND 67% 100%
Neomycin 100% 90% 33% 33% 100% 100% 44% 95% 63% 100% 0% 93%
Orbifloxacin 75% 95% 0% 0% 60% 100% 19% 100% 78% 100% 38% 88% Ormetoprim / Sulfadimethoxine 0% 2% 0% 0% 0% 0% 4% 0% 0% 0% 0% 0%
Oxacillin**** ND ND ND ND ND ND ND ND ND 100% ND ND
Penicillin 0% 0% 100% 25% 0% 0% 0% 100% 33% 60% 100% 31%
Piperacillin ND ND ND 0% ND ND 100% ND ND ND ND 0%
Polymixin B ND ND ND 0% ND ND 100% ND ND ND ND 100%
Rifampin 67% 0% 0% 100% ND 0% 0% 100% 0% ND 100% 100%
Sulfachloropyridazine 80% 86% 17% 0% 60% 100% 7% 86% 75% 80% 80% 79%
Tetracycline 88% 87% 71% 25% 60% 75% 0% 100% 56% 100% 38% 56%
Ticarcillin 100% 92% 0% 0% ND 0% 71% 100% 100% ND 100% 67% Ticarcillin / Clavulanic Acid 100% 100% 0% 0% ND 100% 53% 100% 100% ND 100% 67%
Tobramycin ND ND ND 0% ND ND 100% ND ND ND ND 100% Trimethoprim / Sulphamethoxazole 63% 91% 100% 75% 80% 100% 3% 100% 89% 100% 88% 81%
26
Porcine MIC Susceptibility Profile of Porcine Pathogens Submitted to ISU VDL in 2008
A suis APP B bron E coli Erys H ecol HPS P mul A P mul
D S chol S suis S typm
# tested 308 103 19 803 25 1139 519 742 429 88 1140 368
Percent Susceptible***
Ampicillin 95% 73% 32% 30% 100% 25% 100% 99% 99% 27% 99% 11%
Ceftiofur** 100% 99% 0% 59% 100% 58% 100% 100% 100% 100% 99% 88%
Chlortetracycline 93% 65% 100% 6% 4% 7% 98% 98% 95% 20% 9% 6%
Clindamycin 0% 0% 0% 0% 68% 0% 2% 0% 0% 0% 24% 0%
Danofloxacin 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% 0% ND
Enrofloxacin 100% 98% 100% 96% 100% 100% 100% 100% 100% 100% 97% 99%
Florfenicol** 100% 96% 63% 11% 20% 19% 100% 100% 100% 99% 99% 2%
Gentamicin 95% 4% 95% 67% 0% 60% 93% 97% 97% 100% 92% 75%
Neomycin 83% 3% 95% 63% 0% 55% 78% 98% 97% 100% 54% 72%
Oxytetracycline** 65% 11% 100% 6% 4% 6% 95% 84% 65% 20% 4% 6%
Penicillin 1% 13% 0% 0% 100% 0% 17% 89% 90% 0% 86% 0%
Spectinomycin 0% 1% 0% 0% 56% 1% 62% 2% 1% 0% 9% 0%
Sulfadimethoxine 93% 60% 32% 27% 4% 17% 61% 49% 49% 10% 38% ND
Tiamulin** 33% 94% 0% 0% 56% 1% 86% 51% 8% 0% 90% 0%
Tilmicosin** 88% 92% 21% 0% 88% 0% 90% 92% 31% 0% 27% 0% Trimethoprim / Sulphamethoxazole 96% 3% 47% 75% 48% 77% 96% 95% 0% 100% 98% 82%
Key:
* In vitro antimicrobial test results do not represent therapeutic recommendations from the VDL or personnel therein. Extra/Off label usage of an antimicrobial which is limited/prohibited for certain species may result in legal action by FDA-CVM
** These are the only antimicrobials with valid breakpoints correlated with clinical outcome in species presented.
*** Percent of isolates with a susceptible value.
**** Methicillin resistant is represented by oxacillin.
ND Not done
A equ Actinobacillus equuli H som Histoplilus somni S aur Staphylococcus aureus
A suis Actinobacillus suis HPS Haemophilus parasuis S beta Beta Streptococcus species
Abua Acinetobacter species K pneu Klebsiella pneumoniae S can Streptococcus canis
Amy Actinomyces species M bov Moraxella bovis S chol Salmonella choleraesuis
APP Actinobacillus pleuropneumoniae M haem Mannheimia haemolytica S dubl Salmonella dublin
B bron Bordetella bronchiseptica Non E coli non hemolytic E. coli S dysg Streptococcus dysgalactiae
Bact Bacteriodes group P aer Pseudomonas aeruginosa S epi Staphylococcus epidermidis
C diff Clostridium difficile P cab Pasteurella caballi S equi Streptococcus equi
C perf Clostridium perfringens P mult Pasteurella multocida S equs Streptococcus equisimillis
Clos Clostridium species P tre Pasteurella trehalosi S int Staphylococcus intermedius
E coli Escherichia coli Past Pasteurella species S new Salmonella newport
E fael Enterococcus faecalis Pec Peptocococcus species S schl Staphylococcus schleiferi
E faem Enterococcus faecium Pes Peptostreptococcus species S suis Streptococcus suis
Ecoli 99 - E.coli, K99 negative Pmul A Pasteurella multocida Type A S typm Salmonella typhimurium
Ecoli 99 + E.coli, K99 positive Pmul D Pasteurella multocida Type D S ube Streptococcus uberis
Enc Enterococcus species Prot Proteus species S ugan Salmonella uganda
Ente Enterobacter species Prp Propionibacterium species S zoo Streptococcus zooepidemicus
Erysip Erysipelothrix Pseu Pseudomonas species Salm Salmonella
Fus Fusobacterium R equ Rhodococcus equi Salm B Salmonella species group B
G ana Gallibacterium anatis S aha Alpha hemolytic Streptococcus species Salm C1 Salmonella species group C1
H ecol hemolytic E.coli Salm C2 Salmonella species group C2
27
Bioanalytical Services (BAS)
Bioanalytical Services 5-year Trend 2005 2006 2007 2008
Total number of tests 6,621 20,314 25,539 20,573
PCR Based detection in Blood Swabs 1,541 6,698 7,590 9,846
PCR Based detection in Semen 3,311 5,081 4,282 3,366
PCR Based detection in Serum 1,769 8,535 13,667 7,361
Chemistry/Toxicology (Chem/Tox)
2009 Chemistry/Toxicology Number of Accessions Number of Tests
Total 2910
Vitamin E in tissue 110 325
Trace Mineral Panel in tissue 112 287
Selenium in tissue or feed 128 199
Copper in feed or tissue 54 167
Mycotoxin Panel 84 153
Trace Mineral Panel in serum or water 31 131
Mycotoxin ELISA Screen 3 62 123
Antibiotics - Chlortetracycline - quantitation 57 93
Mycotoxin - individual - quantitation 29 90
Selenium in blood, serum or plasma 26 79
Antibiotics - Tylosin - quantitation 40 75
Vitamin E in serum 26 66
Cholinesterase 42 65
Trace Mineral Panel in feed 14 64
Chemistry/Toxicology 5-year trend 2005 2006 2007 2008 2009
Grand Total 1934 2123 3049 2413 2910
Aflatoxin - qualitative 2 23 6 11 10
Aflatoxin - qualitative M1 Liver - 2 4 1 3
Ammonia Nitrogen 11 8 21 15 19
Antibiotics - Chlortetracycline - quantitation 79 81 140 83 93
Antibiotics - Lincomycin - quantitation 1 5 - - -
Antibiotics - Oxytetracycline - quantitation 8 5 6 6 2
Antibiotics - Penicillin - quantitation 1 5 6 3 18
Antibiotics - Tetracycline - quantitation - 5 4 15 13
Antibiotics - Tylosin - quantitation 81 68 130 81 75
Anticoagulant - Brodifacoum - quantitation - - 1 - -
Anticoagulant - Dicoumarol - quantitation - 1 1 - 1
Anticoagulant - Diphacinone - quantitation - - 2 - -
Anticoagulant - Warfarin - quantitation - - - 1 -
Anticoagulant Panel 8 24 18 14 9
28
Chemistry/Toxicology 5-year trend 2005 2006 2007 2008 2009
Arsenic in feed or tissue 15 10 7 16 12
Arsenic in fluids 1 3 - 7 -
Ash - 1 - 13 -
Barbiturates 2 3 - - -
Bone Ash 2 27 22 16 8
Bone Density 2 16 19 12 4
Cadmium in feed or tissue - 1 - - -
Caffeine 1 - - - -
Calcium in feed or tissue 7 39 26 13 10
Calcium in serum, plasma, eye, water 23 2 - 1 3
Carbamate - individual - qualitative - - - - 1
Carbamate - individual - quantitation - - - 5 3
Carbamate Panel 4 2 14 23 14
Carboxyhemoglobin - 3 2 5 5
Chloride in feed or tissue - 3 - - -
Chloride in fluids 22 1 - - 7
Chlorinated Hydrocarbon - individual - quantitation - - - - -
Chlorinated Hydrocarbon Panel - - 3 2 2
Cholinesterase 21 26 34 91 65
CNS Canine Panel 7 5 2 2 -
Copper in blood 1 - - - -
Copper in feed or tissue 104 170 185 156 167
Copper in liver biopsy 6 18 11 4 4
Copper in serum or water 44 58 132 27 23
Coumestrol - quantitation 2 3 1 - -
Custom Chemistry 60 76 92 131 134
Cyanide 2 3 2 3 5
Ergopeptine Panel 9 9 12 6 9
Ergotamine – qualitative - TLC - - - - -
Ethylene Glycol 5 2 17 5 3
Feed Particle Size 1 2 - 5 4
Fluoride 7 2 1 - 18
Fluoride in tissue 4 2 2 - 2
Fumonisin B1 - qualitative 7 7 12 13 3
Fumonisins ELISA - - - - 1
Gossypol - - 5 1 -
Gossypol - quantitation - - 2 1 1
Hormonally active feed compounds panel 8 1 2 - -
Iron in feed or tissue 3 25 28 26 20
Iron in liver biopsy 1 1 - - -
Iron in serum or water 28 89 48 14 10
Lasalocid - qualitative 12 5 15 16 14
Lead in blood or water 53 40 55 44 57
Lead in feed or tissue 47 34 36 36 64
29
Chemistry/Toxicology 5-year trend 2005 2006 2007 2008 2009
Lead in paint 1 - - - -
Magnesium in feed or tissue 1 7 1 2 -
Magnesium in fluids 29 2 - 1 3
Manganese in feed or tissue 6 57 92 75 11
Manganese in serum or water 5 1 14 2 2
Melengesterol Acetate - quantitation 1 2 8 5 12
Mercury in blood 1 - 1 2 -
Mercury in feed or tissue - 1 - 6 2
Methemaglobin - - 28 5 -
Microtox Test 2 - - - -
Moisture 13 15 71 94 34
Molybdenum in feed or tissue 2 8 4 3 1
Monensin - qualitative 19 12 76 34 20
Monensin - quantitation 10 9 22 17 8
Mycotoxin - individual - quantitation 22 30 21 32 90
Mycotoxin DDGS Panel - - - - 2
Mycotoxin ELISA Screen 1 - - - - 1
Mycotoxin ELISA Screen 2 - - - - 30
Mycotoxin ELISA Screen 3 - - - - 123
Mycotoxin Panel 61 96 132 73 153
Nitrates in feed 7 8 8 18 4
Nitrates in serum or eye fluid 23 7 43 27 36
Nitrite analysis - - - - 3
Nitrite or Nitrate in water 28 2 - 11 13
Ochratoxin - qualitative - 1 - 1
Organophosphate – individual - qualitative 1 15 2 1 3
Organophosphate - quantitation 2 14 5 2 2
Organophosphate Panel 25 16 19 24 10
pH 38 8 8 12 20
Phenylarsonic compounds 1 - - - -
Phosphorus in feed or tissue 6 37 16 12 8
Phosphorus in fluids 5 - - - -
Potassium in fluids 3 2 - - -
Selenium in blood, serum or plasma 232 193 214 149 79
Selenium in tissue or feed 148 219 303 316 199
Slaframine 4 1 0 2 2
Sodium in feed or tissue 4 11 14 41 11
Sodium in fluids 23 1 2 - 6
Strychnine - 3 8 - 1
Sulfa Drug Panel - - 1 4
Sulfate in feed 4 28 63 65 27
Sulfate in water 27 20 27 28 20
Sulfonamide - qualitative 3 - - 3 -
T-2 toxin - qualitative - 2 - - -
30
Chemistry/Toxicology 5-year trend 2005 2006 2007 2008 2009
Total Dissolve Solids 1 - - 3 2
Trace Mineral Panel in feed - - - - 64
Trace Mineral Panel in serum or water - - - - 131
Trace Mineral Panel in tissue - - - - 287
Tremorgen panel 5 3 2 - 2
Urea 3 9 10 1 -
V9 mineral panel 49 26 58 29 12
Vitamin A acetate - - - 1 -
Vitamin A in serum 5 13 14 9 14
Vitamin A palmitate in feed - quantitation - - - 4 -
Vitamin A palmitate in liver - quantitation 2 4 14 4 6
Vitamin D3 in feed 11 10 1 - 6
Vitamin E acetate in feed - quantitation 7 1 3 11 25
Vitamin E in liver 116 127 235 -
Vitamin E in serum 207 108 289 71 66
Vitamin E in tissue - - - 175 325
Vomitoxin - qualitative 2 9 2 25 29
Vomitoxin ELISA - - - - 3
Water Quality Panel 28 32 42 35 55
Zearalenone ELISA - - - - 1
Zearalenone/Zearalenol - qualitative 7 3 3 13 14
Zinc in feed or tissue 23 24 30 31 9
Zinc in liver biopsy 2 1 - - -
Zinc in serum or urine 7 9 17 10 7
2008 Toxicology diagnosis by species Bovine Canine
Caprine/ Ovine Equine Feline Porcine Total
Ethylene glycol (antifreeze) 5 1 6
Water deprivation, Sodium Ion Toxicosis 7 7
Copper 4 4
Lead 4 4
Organophosphate 1 2 3
Carbamate 1 1 2
Carbofuran (Furadan) 1 1 2
Ionophore 2 2
Monensin 2 2
Idiopathic 1 1
Arsenic 1 1
Hydrogen sulfide 1 1
Nitrate 1 1
Oak 1 1
Phorate (Thimet) 1 1
31
32
Molecular Diagnostics
Molecular Diagnostics 5-year Trends 2004 2005 2006
2007
2008
Total number of tests 21,038 39,645 55,458 64,223 73,625
PCR 19,418 37,830 49,467 58,290 67,479
Actinobacillus pleuropneumoniae 2 - 1 - 1
Avian influenza virus - - - - 1,545
Bovine coronavirus - - - - 1,228
Brachyspira hyodysenteriae or B.pilosicoli - - - - 156
BRSV - - - - 245
BVDV fluid - - - - 255
BVDV genotyping - - - - 5
BVDV tissue/blood - - - - 229
Calf diarrhea panel testing - - - - 470
Canine distemper virus - - - - 9
Clostridium perfringens genotyping 213 180 148 92 218
CSFV surveillance - - - - 443
E. coli genotyping 145 406 366 215 880
Haemophilus parasuis 65 95 112 42 267
Lawsonia intracellularis 910 756 338 167 384
Leptospira - 390 556 369 507
Leptospira hardjo-bovis - - - - 60
M. paratuberculosis (Johne’s) - - - - 315
M. bovis - - - - 386
M. hyopneumoniae 1,120 2,261 3,676 1,709 4,339
M. hyopneumoniae (mhp165/mhp183) - - - - 4
Pasteurella multocida 73 36 25 - 45
PCV2 - fluid - - - - 5,170
PCV2 - tissue - - - - 511
PCV ORF2 - - - - 27
Porcine parvovirus - - - - 269
Porcine teschovirus - - - - 66
PRRSV - fluid - - - - 26,757
PRRSV - tissue - - - - 8,196
PRRSV ORF5 - - - - 1,156
Rabies virus - - - - 1
SIV - - - - 6,581
SIV HA gene - - - - 204
TGE/PRCV - - - - 1,251
WNV - - - - 12
Other / Undifferentiated * 16,890 33,706 44,245 55,696 493
Sequencing and Analysis * 569 874 1,399 1,598 1,341
PCV2 - - - - 21
PCV ORF2 Sequencing - - - - 4
PRRSV - - - - 1,184
SIV - - - - 131
Other / Undifferentiated* - - - - 1
Quantitation * 1,051 941 4,592 4,335 4,805
PCV2 - - - - 2,501
PRRSV - - - - 2,289
Other / Undifferentiated* - - - - 15
* Before 2008, number of tests for each specific pathogen is not available.
33
Pathology
Pathology 5-year Trend 2004 2005 2006
2007
2008
Total number of tests 82,649 91,476 98,922 124,094 107,941
Biopsy Hematoxylin and Eosin Slides 1,061 1,296 1,225 1,231 1,202
Hematoxylin and Eosin Slides 29,199 34,701 40,342 40,811 36,702
Fecal Flotation 260 307 316 301 241
Immunohistochemistry 52,030 55,245 57,168 78,197 66,471
Immunohistochemistry - BVD PI Biopsy 36,708 38,184 36,901 55,215 50,495
Immunohistochemistry - Bovine 1,752 2,900 1,931
Immunohistochemistry - Porcine 13,043 19,020 12,576
Immunohistochemistry - Unclassified 15,322 17,061 5,472 1,062 1,469
Necropsy 1,335 2,360 1,174 2,696 2,758
Parasite identification 15 9 6 15 11
Special Stains 391 234 420 843 476
Serology
Serology 5-year Trends 2004 2005 2006
2007
2008
Total number of tests 643,077 691,573 984,335 707,628 831,745
Anaplasma cELISA 3,851 1,495 5,965 11,454 11,117
Anaplasma marginale CARD 1 - - 1,383 332
Anaplasma marginale CF 661 807 768 553 499
APP ApxIV Elisa - - - - 705
APP CF screen (no serotyping) 10,791 9,206 7,282 5,667 22,146
APP CF serotype 1 only 204 73 4 11 5
APP CF serotype 3 only 4,260 3,958 2,347 2,569 18,688
APP CF serotype 5 only 220 20 78 11 48
APP CF serotype 7 only 359 143 186 81 3
APP CF serotype 157 172 432 577 130 15
Avian influenza AGID 8,605 88 27,691 33,127 22,752
Avian influenza ELISA 2,280 98 766 481 926
Avian pneumovirus ELISA 1,591 6,217 7,613 4,367 358
Bluetongue virus AGID 571 677 735 1,024 589
Bluetongue virus cELISA - - - 124 218
Bovine coronavirus IFA 2 3 - - -
Bovine leukemia virus AGID 3,505 2,861 9,190 15,205 13,539
BRSV VN 760 564 794 586 443
Brucella BAPA screen 1,149 1,626 7,115 19,608 25,798
Brucella canis RSAT 950 1,507 957 404 427
Brucella card test 1,962 3,114 3,127 4,388 6,413
Brucella RAP 9,428 5,685 7,188 2,845 212
Brucella RIV 500 167 444 214 206
34
Serology 5-year Trends 2004 2005 2006
2007
2008
Brucella SPT 823 464 492 745 638
Brucella STT 802 1,258 1,435 2,230 4,614
BVD type 1 VN 1,983 2,329 2,411 2,416 2,775
BVD type 2 VN 1,803 1,952 2,028 1,948 1,863
Canine herpesvirus VN 14 61 19 2 16
Caprine arthritis encephalitis AGID 111 307 646 387 149
EHD virus AGID 620 597 630 611 574
EIA virus AGID 5,970 4,971 2,595 2,153 1,776
EIA virus ELISA - 210 2,378 2,361 2,268
EMC virus VN 97 - - - -
Feline leukemia virus ELISA - 4 - - -
FIV ELISA 3 2 - - -
Hemophilus somnus CF 470 369 295 302 185
IBR (BHV-1) VN 2,126 1,850 1,894 1,649 2,029
Johne’s (bovine) CF 459 496 690 521 486
Johne’s (bovine) ELISA 14,942 10,350 21,075 25,938 23,092
Johne’s (ovine) AGID 70 186 124 550 53
Leptospira 5 MAT 2,305 2,945 2,746 2,679 3,093
Leptospira 6 MAT 4,528 5,797 4,501 3,466 3,765
Leptospira australis MAT 292 343 285 288 355
Leptospira autumnalis MAT 3 3 1 28 90
Leptospira ballum MAT 292 337 285 288 355
Leptospira bataviae MAT 1 - - - -
Leptospira bratislava MAT 28 28 142 107 364
Leptospira canicola MAT 787 1 65 63
Leptospira copenhageni MAT 3 - - - -
Leptospira grippotyphosa MAT 795 1 89 1 -
Leptospira hardjo MAT 123 1,150 135 647 593
Leptospira hebdomadi MAT 12 10 - - -
Leptospira ictero MAT - 1 65 - -
Leptospira pomona MAT 1 - 155 5 -
Leptospira pyrogenes MAT 1 - - - -
Leptospira sejroe MAT 417 462 285 291 739
Leptospira swazjak MAT 1 - - - -
Leptospira tarassovi MAT 292 428 285 288 355
M hyopneumoniae CF 20 13 12 166
M hyopneumoniae DAKO ELISA 1,020 2,018 2,492 2,950 12,008
M hyopneumoniae ELISA 22,719 25,956 30,246 29,162 27,637
M hyopneumoniaeTween 20 - - - - 4,251
Neospora caninum ELISA 978 906 6,097 11,769 10,040
Ovine progressive pneumonia AGID 66 93 93 60 31
Parainfluenza 3 Virus VN 623 459 752 602 358
PCV 2 - 4 dilution - - - - 4,100
PCV 2 - cELISA 118 1,501 22,520 36,522 9,376
PCV 2 - Differential ELISA - - - - 12,453
PCV 2 - ELISA - - - - 10,043
35
Serology 5-year Trends 2004 2005 2006
2007
2008
PCV 2 - FFN - - - 605 242
PCV 2 - IFA endpoint titration - - - - 183
PCV 2 - IFA screen 3,393 3,685 6,296 3,574 934
PCV 2 - IFA titer 10 161 98 1,557 1,787
PCV 2 - IgG Elisa - - - - 48
PCV 2- IgM Elisa - - - 426 -
Porcine parvovirus HI 4,078 4,292 5,791 3,989 3,000
PRRS ELISA 87,498 153,916 351,677 157,031 146,489
PRRS ELISA (meat juice surveillance) - - - 27,993 96,770
PRRS FFN 643 468 331 444 325
PRRS IFA 2,537 4,495 2,827 2,919 606
PRRS IFA European 411 1,435 1,738 1,616 1,379
PRRS IFA North American - - - - 1,456
PRRS IFA titration - - - 1 1
PRRS IFA titration North American - - - - 3
PRV ALA SCREEN 881 539 1,803 1,773 2,114
PRV ELISA SCREEN 3,002 2,692 796 - -
PRV g1 ELISA 325,532 304,344 299,816 178,017 83,115
PRV gB ELISA SCREEN - - 765 1,900 1,467
PRV VN 1,382 1,844 2,813 2,938 5,497
Pullorum - typhoid AGG 120 7 57 - 32
Swine influenza virus (Homologous) HI - 380 82 46 -
Swine influenza virus H1N1 ELISA 2,145 3,246 18,349 15,579 12,301
Swine influenza virus H1N1 HI 28,036 15,045 191 20 -
Swine influenza virus H1N1 HI (31) Pfizer - - - - 99
Swine influenza virus H1N1 HI (73) - - - - 8
Swine influenza virus H1N1 HI (99) - 9,112 23,899 13,193 8,006
Swine influenza virus H1N1 HI Pfizer 11,236 12,045 7,967 4,850 3,025
Swine influenza virus H3N2 ELISA - 1,310 7,206 8,659 8,078
Swine influenza virus H3N2 HI 48,544 52,412 43,853 22,606 6,430
Swine influenza virus H3N2 HI C1/3 - - - - 7,035
Swine influenza virus H3N2 HI C4 2007013431 - - - - 99
Swine influenza virus NP Elisa - - 5 968 1,490
Swine influenza virus NS1 Elisa - - 47 155 -
TGE virus VN 1,470 1,906 1,659 2,213 7,475
TGE/PRCV 2,689 8,344 11,824 14,968 13,530
Toxoplasma gondii ELISA - - - - 74,659
Toxoplasma LAT 164 474 340 420 235
Trichinella spiralis ELISA - - - - 74,708
VS virus Indiana strain VN 844 1,466 1,642 1,951 1,442
VS virus New Jersey strain VN 836 1,466 1,643 1,950 1,442
West Nile Virus Capture IgM ELISA 86 80 71 69 43
36
Virology
Virology 5-year Trends 2004 2005 2006
2007
2008
Total number of tests 5,436 6,089 23,800 9,000 9,319
Antigen ELISA 1,970 2,102 20,354 5,970 6,090
FA test 1,061 712 728 740 1,668
Rabies 1,236 1,095 975 1,031 1,049
Virus isolation exam 1,152 2,129 1,735 1,254 1,365
Molecular & Virology Tests Conducted by Agent 2008
PRRS virus 36,367
SIV 6,969
PCV2 5,720
Mycoplasma hyopneumoniae 4,339
Rotavirus 2,086
Avian influenza virus 1,545
TGE/PRCV 1,256
Bovine coronavirus 1,230
Rabies 1,049
BVD 1,038
E. coli 880
Other 627
Leptospira 507
Calf diarrhea 470
Mycoplasma bovis 386
Lawsonia intracellularis 384
Mycoplasma paratuberculosis (Johne’s) 315
Porcine parvovirus 300
Haemophilus parasuis 267
BRSV 255
Clostridium perfringens 218
SIV HA gene 204
IBR virus (BHV-1) 177
Brachyspira hyodysenteriae 156
Bovine Herpesvirus type 1 87
PRV 80
Canine parvovirus 76
Canine distemper 74
Porcine Teschovirus 66
Leptospira hardjo-bovis 60
Canine herpesvirus 53
Pasteurella multocida 45
Canine adenovirus 31
Equine herpesvirus type 1&4 26
Feline calicivirus 18
37
Molecular & Virology Tests Conducted by Agent 2008
Feline herpesvirus 18
Porcine enterovirus 14
Feline parvovirus 13
Canine coronavirus 12
WNV 12
Bovine parainfluenza virus type 3 10
Encephalomyocarditis virus 8
Feline infectious peritonitis virus 7
Influenza A virus 6
M. hyopneumoniae (mhp165/mhp183) 4
HEV 3
Actinobacillus pleuropneumoniae 1
Equine influenza virus 1
2008 Virology Number of Accessions Number of Tests
total 3,864 9,319
ELISA 6,090
BVD virus AgELISA 925 3,831
BVD virus AgELISA modified 85 156
Type A Rotavirus antigen-capture ELISA 1,023 2,086
Influenza A virus AgELISA 4 6
Parvovirus AgELISA 10 11
Electron Microscopic Exam 1 2
FA 1,668
FA - Bovine coronavirus 2 2
FA - Bovine Herpesvirus type 1 80 87
FA - Bovine parainfluenza virus type 3 3 10
FA - Bovine respiratory syncytial virus 3 10
FA - Bovine viral diarrhea virus 3 10
FA - Canine adenovirus 28 29
FA - Canine coronavirus 11 12
FA - Canine distemper 55 65
FA - Canine herpesvirus 41 46
FA - Canine parvovirus 67 76
FA - Encephalomyocarditis virus 1 1
FA - Equine herpesvirus type 1&4 20 21
FA - Feline calicivirus 14 15
FA - Feline herpesvirus 14 15
FA - Feline infectious peritonitis virus 7 7
FA - Feline parvovirus 11 12
FA - HEV 2 3
FA - Porcine parvovirus 17 19
FA - PRV 35 38
FA - Rabies virus 1,044 1,049
FA - Rabies weekend/after hours testing 32 32
38
FA - Transmissible gastroenteritis virus 2 2
FA - Other 63 76
Virus and Sample Return 133 181
Virus isolation 1,365
VI - Bovine viral diarrhea virus 100 383
VI - Canine adenovirus 2 2
VI - Canine herpesvirus 4 7
VI - Encephalomyocarditis virus 2 7
VI - Equine herpesvirus type 1&4 4 5
VI - Equine influenza virus 1 1
VI - Feline calicivirus 3 3
VI - Feline herpesvirus 3 3
VI - Feline parvovirus 1 1
VI - IBR virus (BHV-1) 57 177
VI - Porcine circovirus type 2 9 12
VI - Porcine enterovirus 9 14
VI - Porcine parvovirus 1 1
VI - Porcine respiratory coronavirus 2 3
VI - PRRS virus 155 258
VI - Pseudorabies virus 34 42
VI - Swine influenza virus 308 388
VI - Other 20 58
Virus propagation 4 13
2008 Rabies Accessions Negative Positive Inconclusive Unsuitable Total
Grand Total 990 17 18 24 1,049
Feline 314 5 5 4 328
Bat 270 6 12 288
Canine 219 1 2 3 225
Procyonidae 59 2 1 62
Bovine 53 1 2 3 59
Rodent 28 1 1 30
Equine 18 18
Skunk 5 4 3 12
Marsupial 7 3 10
Canine Exotic 4 4
Lagomorpha 4 4
Caprine 3 3
Ovine 3 3
Cervidae 1 1
Exotic 1 1
Mole 1 1
39
2008 Rabies Accessions by Month Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec Total
Grand Total 45 45 65 72 67 115 120 158 115 94 77 76 1049 Feline 9 11 20 21 21 32 48 32 36 38 29 31 328 Bat 15 13 9 13 12 32 10 95 42 11 12 24 288 Canine 10 9 16 20 18 25 33 17 21 30 15 11 225 Procyonidae 2 2 8 5 3 7 8 4 7 6 9 1 62 Bovine 2 5 7 9 8 7 9 4 3 3 2 59 Rodent 3 2 1 2 2 4 5 1 3 2 5 30 Equine 2 4 3 4 2 1 2 18 Skunk 1 1 2 1 2 2 1 2 12 Marsupial 1 1 1 2 2 1 2 10 Canine Exotic 1 1 2 4 Lagomorpha 1 1 1 1 4 Caprine 1 1 1 3 Ovine 1 1 1 3 Cervidae 1 1 Exotic 1 1 Mole 1 1
Rabies Positive Trends 2004 2005 2006 2007 2008
Total submissions 1,236 1,095 975 1,031 1,049
Total positives 81 101 41 22 17
% positive 7% 9% 4% 2% 1.6%
Number of positive animals
Bat 35 43 19 7 6 Skunk 26 29 12 4 4 Feline 6 21 5 6 5 Bovine 9 6 2 - 1 Canine 4 2 2 4 1 Equine - - 1 1 0 Canine Exotic 1 - - - 0
45 45
65 72 67
115 120
158
115
9477 76
0 0 1 0 4 0 2 4 1 5 0 0
Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec
2008 Rabies Accessions
Number of Rabies Cases Positives
40
Honors and Awards Boor, Jessica. Employee of the Month, October 2008, ISU College of Veterinary Medicine Holdredge Jennifer, Lee Chris, Krumm Karol, Knosby Renee, Miller Beth, Witte Courtney: 2008 Service
Team of the Year Award. ISU College of Veterinary Medicine. Meetz, Mike. Employee of the Month, August 2008, ISU College of Veterinary Medicine Gauger, Phil Golden Key International Honor Society
Phi Kappa Phi Honor Society Gamma Sigma Delta Honor Society of Agriculture The National Scholars Honor Society
Imerman, Paula 25 year service award Irwin, Christa $12,000 Alex Hogg Scholarship award from AASV foundation and Mary Lou Hogg Kim, Wonil Diplomate of American College of Veterinary Microbiology (DACVM) Kinyon, Joann Employee of the Month, February 2008, ISU College of Veterinary Medicine Madson, Darin The American College of Veterinary Microbiologists Student Award (Don Kahn Award) for best
student presentation, Conference of Research Worker in Animal Disease Iowa Pork Producers Association, Graduate Fellowship Award Yoon, Kyoungjin Experiment Station Award for Excellence in Multistate Research (NC229), National Association of
State Universities and Land-Grant Universities Elected as a Board member of Governors of American College of Veterinary Microbiologists
Selected Papers and Presentations
Refereed Publications Feagins AR, Opriessnig T, Guenette DK, Halbur PG, and Meng. 2008. Inactivation of infectious
hepatitis E virus present in commercial pig livers sold in local grocery stores in the United States. International Journal of Food Microbiology. 123:32-37.
Feagins AR, Opriessnig T, Huang YW, Halbur PG, and Meng XJ. 2008. Cross-species infection of specific-pathogen-free pigs by a genotype 4 strain of human hepatitis E virus. Journal of Medical Virology. 80:1379-1386.
Gillespie J, NM Juhan, J DiCristina, KF Key, S Ramamoorthy and XJ Meng. A genetically engineered chimeric vaccine against porcine circovirus type 2 (PCV2) is genetically stable in vitro and in vivo. 2008. Vaccine. Aug 5; 26(33):4231-6
Hermann JR, Brockmeier SL, Yoon K-J, Zimmerman JJ. 2008. Detection of respiratory pathogens in air samples from acutely infected animals. Can J Vet Res 72:367-370.
Hyde, W., D. Grelich and T. Veritti, “High Sensitivity Drug Screening using GC x GC TOF MS with Spectral Deconvolution Techniques.”, pp. 184-191, 2006 Proceedings* ICRAV, published in 2007, distributed in 2008.
Kim WI and Yoon K-J. 2008. Molecular assessment of the role of envelope-associated structural proteins in cross neutralization among different PRRS viruses. Virus Genes 37:380-391.
Kim WI, Kim JJ, Cha SH, and Yoon K-J. 2008. Identification of the different biological characteristics between wild-type PRRS viruses and vaccine viruses and the corresponding genetic determinants. J Clin Microbiol 46:1758-1768.
Ma W, Lager KM, Richt JA, Stoffregen WC, Zhou F, Yoon K-J. 2008. Development of a real-time PCR for rapid detection and differentiation of field pseudorabies (Ajezsky’s disease) and vaccine viruses. J Vet Diagn Invest 20: 440-447.
41
Madson DM, Loynachan AT, Kariyawasam S, and Opriessnig T. 2009. Systemic Conidiobolus incongruus infection and hypertrophic osteopathy in a white-tailed deer (Odocoileus virginianus). Journal of Veterinary Diagnostic Investigation. 21.
Madson DM, Ramamoorthy S, Kuster C, Pal N, Meng XJ, Halbur PG, Opriessnig T: Characterization of shedding patterns of porcine circovirus type 2a and PCV2b in inoculated mature boars. Journal of Veterinary Diagnostic Investigation. 20:725-734, 2008.
Madson DM, S Ramamoorthy, C. Kuster, N Pal, XJ Meng, PG Halbur, T Opriessnig. 2008. Infectivity of porcine circovirus type 2 DNA in semen from experimentally- infected boars. Veterinary Research 2008 Oct 31; 40(1):10.
Molina RM, Cha S-H, Chittick W, Lawson S, Murtaugh MP, Nelson EA, Christopher-Hennings J, Yoon K-J, Evans R, Rowland RRR, Zimmerman JJ. 2008. Immune response against porcine reproductive and respiratory syndrome virus dueing acute and chronic infection. Veterinary Immunology and Immunopathology. 126:283-292.
Opriessnig T, Madson DM, Prickett JR, Kuhar D, Lunney JK, Elsener J, Halbur PG. Effect of porcine circovirus type 2 (PCV2) vaccination on porcine reproductive and respiratory syndrome virus (PRRSV) and PCV2 coinfection. Veterinary Microbiology. 131:103-114, 2008.
Opriessnig T, Patterson AR, Elsener, J, Meng XJ, and Halbur PG. 2008. Influence of porcine circovirus type 2 (PCV2) maternal antibodies on efficacy of PCV2 vaccination to protect pigs from experimental infection with PCV2. Clinical and Vaccine Immunology. 15:397-401.
Opriessnig T, Patterson AR, Madson DM, Pal N, and Halbur PG. 2009. Comparison of efficacy of commercial one dose and two dose PCV2 vaccines using a mixed PRRSV-PCV2-SIV clinical infection model 2-3 month post vaccination. Vaccine. Available online.
Opriessnig T, Patterson AR, Meng XJ, and Halbur PG. 2009. Porcine circovirus type 2 in muscle and bone marrow is infectious and transmissible to naïve pigs by oral consumption. Veterinary Microbiology. 133:54-64,
Opriessnig T, Ramamoorthy S, Madson DM, Patterson AR, Pal N, Carman S, Meng XJ, and Halbur PG. 2008. Porcine circovirus type 2a and 2b isolates are similar in virulence and prior infection with either 2a or 2b isolate provides heterologous protection. Journal of General Virology. 89:2482-2491.
Opriessnig T, S Ramamoorthy, DM Madson, AR Patterson, , X. J. Meng, PG Halbur. Experimental comparison of the virulence of PCV2a and PCV2b isolates in a conventional pig model and evaluation of cross-protection between isolates. J Gen Virol. 2008 Oct; 89(Pt 10):2482-91.
Pal N, Huang WY, Madson DM, Kuster C, Meng XJ, Halbur PG, Opriessnig T: Development of a duplex real-time PCR for the simultaneous detection and quantification of porcine circovirus type 2 and an internal control on porcine semen samples. Journal of Virological Methods. 149:217-225, 2008.
Patel D, Opriessnig T, Stein DA, Halbur PG, Meng XJ, Iversen PL, and Zhang YJ. 2008. Characterization of Morpholino Oligomer Inhibition of Porcine Reproductive and Respiratory Syndrome Virus. Antiviral Research. 77:95–107.
Patterson AR, Johnson J, Ramamoorthy S, Meng XJ, Halbur PG, and Opriessnig T. 2008. Comparison of the ability of three ELISAs to detect porcine circovirus-2 (PCV2)-specific antibodies after vaccination or inoculation with genetically distinct PCV1 or PCV2 isolates. Journal of Veterinary Diagnostic Investigation. 20:744-751.
Pogranichniy RM, Schwartz KJ, Yoon K-J. 2008. Isolation of a novel viral agent associated with porcine reproductive and neurologic syndrome and reproduction of the disease. Vet Microbiol 131:35-46.
Prickett J, Simer R, Christopher-Hennings J, Yoon K-J, Evans RB, Zimmerman JJ. 2008. Detection of porcine reproductive and respiratory syndrome virus infection in porcine oral fluid samples: A longitudinal study under experimental conditions. Journal of Veterinary Diagnostic Investigation 20:156-163.
Prickett JR, Kim WI, Simer R, Yoon K-J, and Zimmerman JJ. 2008. Surveillance of commercial growing pigs for PRRSV and PCV2 infections using pen-based oral fluid samples: A pilot study. Journal of Swine Health Prod 16:86-91.
Pudupakam RS, Huang YW, Opriessnig T, Halbur PG, Pierson FW, and Meng XJ. 2009. Deletions of the hypervariable region (HVR) in open reading frame 1 of hepatitis E virus do not abolish virus infectivity: evidence for attenuation of HVR deletion mutants in vivo. Journal of Virology. 83:384-395.
42
Ramamoorthy S, X.J. Meng. 2008. Porcine circovirus: A minuscule yet mammoth paradox. Animal Health Research Reviews. 2008 Sep 2:1-20.
Ran ZG, Chen XY, Guo X, Ge NX, Yoon K-J, Yang HC. 2008. Recovery of viable porcine reproductive and respiratory syndrome virus from an infectious clone containing partial deletion within Nsp2-encoding region. Archives of Virology 153:899-907.
Rapp-Gabrielson VJ, Hoover T, Sornsen S, Kesl L, Taylor L, Jolie R, Runnels P, Weigel D, Yu S, Opriessnig T, Ruebling-Jass K, Strait E, and Halbur PG. 2008. Effect of Mycoplasma hyopneumoniae vaccination in pigs co-infected with M. hyopneumoniae and porcine circovirus type 2. Journal of Swine Health and Production. 16:16-26.
Reed MC, O’Connor AM, Yoon K-J, Cooper VL. 2008. Assessing the effect of sample handling on the performance of a commercial bovine viral diarrhea virus antigen-capture enzyme-linked immunosorbent assay. J Vet Diagn Invest 20:124-126.
Schleining , J. A., S. R. McClure, R. B. Evans, W. G. Hyde, L. W. Wulf, A. J. Kind, “Liposome-Based Diclofenac for the Treatment of Inflammation in an Acute Synovitis Model in Horses: Effectiveness of Topical Diclofenac in Horses, Journal of Veterinary Pharmacology and Therapeutics, 31, 554-561, doi: 10.1111/j.1365-2885.2008.00994.x.
Vincent AL, Lager KM, Janke BH, Gramer MR, Richt JA. 2008. Failure of protection and enhanced pneumonia with a US H1N2 swine influenza virus in pigs vaccinated with an inactivated classical swine H1N1 vaccine. Veterinary Microbiology. Jan 25;126(4):310-23.
Vincent AL, Ma W, Lager KM, Janke BH, Richt JA. 2008. Swine influenza viruses: A North American perspective. Advances in Virus Research 72:127-154. (Note: Cover photomicrograph for Volume 72 by Janke BH)
Refereed publications accepted but not in print Arther RG, Atkins C, Ciszewski DK, Davis WL, Ensley SM, Settje TL (2008) Safety of Advantage
Multi™ (10% imidacloprid + 1% moxidectin) Topical Solution Applied to Cats Infected with Adult Heartworms (Dirofilaria immitis) Using a Natural Infection Model. Veterinary Parasitology, in press.
Atkins CE, RG Arther, DK Ciszewski, WL Davis, SM Ensley et. al. 2008. Echocardiographic Quantification of Dirofilaria immitis in Experimentally-Infected Cats. Veterinary Parasitology. Dec 10; 158(3):164-70.
Baker RB, Madson DM, Patterson AR, Opriessnig T: Evaluation of the safety of different porcine circovirus type 2 (PCV2) tissue homogenate vaccines in a pig bioassay. Veterinary Record. Accepted.
Ma W, Lager KM, Vincent AL, Richt JA, Janke BH, Gramer MR. 2008. The role of swine in the generation of novel influenza viruses. Zoonoses and Public Health.
Madson DM, and Opriessnig T. Multicentric T-cell lymphosarcoma in a white-tailed deer. Journal of Wildlife Disease. Accepted.
Madson DM, Loynachan AT, Kariyawasam S, Opriessnig T: Systemic Conidiobolus incongruous and hypertrophic osteopathy in a white-tailed deer (Odocileus virginianus). Journal of Veterinary Diagnostic Investigation. In Press.
Madson DM, Patterson AR, Pal N, Ramamoorthy S, Meng XJ, and Opriessnig T. Reproductive failure experimentally induced in sows via artificial insemination with semen spiked with porcine circovirus type 2 (PCV2). Veterinary Pathology. Accepted.
Madson DM, Ramamoorthy S, Kuster C, Pal N, Meng XJ, Halbur PG, Opriessnig T: Infectivity of porcine circovirus type 2 DNA present in semen of experimentally infected boars. Veterinary Research. In Press.
Opriessnig T, AR Patterson, DM Madson, N Pal, PG Halbur: Comparison of efficacy of commercial one dose and two dose PCV2 vaccines using a mixed PRRSV–PCV2–SIV clinical infection model 2–3-month post vaccination. Vaccine. In press
Opriessnig T, Patterson A, Jones, Juhan N, Meng XJ, and Halbur P. Susceptibility of three different mouse (Mus musculus) lines to porcine circovirus type 2 infection and associated lesions. The Canadian Journal of Veterinary Research. In press.
Opriessnig T, Patterson AR, Madson DM, Pal N, Rothschild M, Kuhar D, Lunney J, Juhan NM, Meng XJ, and Halbur PG. Difference in severity of porcine circovirus type 2 (PCV2)-induced
43
pathological lesions between selected Landrace and Pietrain pigs. Journal of Animal Science. Accepted.
Phung, K, A Kind, J Peterson, L Wulf and W Hyde, “Developing the optimum LC/MS technique for confirmation of recombinant human erythropoietin and darbepoetin alfa in equine plasma samples”, Proceeding*, 17th International Conference of Racing Analysts and Veterinarians, Antalya, Turkey, R & W Communications, 2008.
Soring, KH, and W Hyde, “Review of Anabolic Steroid Testing in a Racing Environment.” American Association Equine Practitioners, vol. 54, 2008. *The ICRAV Proceedings are peer reviewed and refereed.
Thacker, ET, Janke BH. 2008. Swine influenza virus: Zoonotic potential and vaccination strategies for the control of avian and swine influenzas. . J Infect Dis. Adapted from proceedings of the Harvard University Asian Flus and Avian Influenza Workshop, Harvard University, Cambridge, MA, Dec 8-9, 2006.
Zhang Y, J Caupert, J Richard, P Imerman, and J Shurson, Dec. 2008. US Grain Council, White Paper: Scientific Overview of the Presence and Levels of Mycotoxins in U.S. DDGS.
Books or book chapters published Ensley S: 2007, Organochlorines, In “Veterinary Toxicology: Basic & Clinical Principles” (R. Gupta,
Ed.), Elsevier. Ensley S: 2007, Pyrethrins, Pyrethroids and Other Insecticides, In “Veterinary Toxicology: Basic &
Clinical Principles” (R. Gupta, Ed.), Elsevier. Ensley S: 2007, Water Quality for Animals, In “Veterinary Toxicology: Basic & Clinical Principles”
(R. Gupta, Ed.), Elsevier. Irwin, Christa, NPB On-Farm Swine Euthanasia (Manual) Osweiler, GD, Chocolate Toxicity in Blackwell’s Five Minute Veterinary Consult, 4th Edition (Tilley
L.P. and Smith, FWK. (eds.) Blackwell, 2008. Osweiler, GD, Ethanol Toxicity in Blackwell’s Five Minute Veterinary Consult, 4th Edition (Tilley L.P.
and Smith, FWK. (eds.) Blackwell, 2008. Osweiler, GD, Carbon Monoxide Poisoning in Blackwell’s Five Minute Veterinary Consult, 4th
Edition (Tilley LP and Smith, FWK. (eds.) Blackwell, 2008. Osweiler, GD, Consulting Editor for Toxicology in Blackwell’s Five Minute Veterinary Consult, 4th
Edition (Tilley L.P. and Smith, FWK. (eds.) Blackwell, 2008. Organized authors, edited manuscripts from authors for 40 topic chapters in 4th edition.
Nonrefereed articles including proceedings Bear DA, Cho Y-I, Russell JR, Ensley SM, Yoon KJ. 2009. Incidence of bovine enterovirus,
coronavirus, and group A rotavirus, and concentration of fecal coliforms in Midwestern pasture streams. ISU Animal Science Animal Industry Report, AS Leaflet R-??? (in press).
Bender J, Kinyon J, Irwin C, and Opriessnig T. 2008. Erysipelothrix rhusiopathiae: Comparison of diagnostic tools and characterization of recent outbreaks. 47th North Central Conference of Veterinary Laboratory Diagnosticians. Madison, Wisconsin, pp. 40-42, June 5-6.
Bender J, Kinyon J, Schwartz K, and Opriessnig T. 2008. Erysipelas: Condemnations and outbreak investigation. 16:36-39. 16th Annual Swine Disease Conference for Swine Practitioners. Ames, IA, November 6-7.
Bosworth B, Erdman M, Winter P, Stine D, Irwin C, Jens M, Loynachan A, Harris DL, Ownes G, Kamrud K. 2009. Evaluation of a virus-like replicon particle vaccine expressing proteins of SIV in pigs with and without maternally derived antibodies. AASV Annual Conference, Dallas, Texas.
Cha S-H, Dorman KS, Kim W-I, Yoon KJ. 2008. Viral recombination among field isolates of PRRSV type 2 – implication for molecular epidemiology. Proceedings, International PRRS Symposium, p81. Chicago, IL; December.
Cha S-H, Dorman KS, Kim WI, Yoon K-J. 2008. Occurrence of recombination among field isolates of PRRSV type 2 – Confusion to molecular epidemiology. International PRRS Symposium, Chicago, IL; December.
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Cho Y-I, Russell JR, Kim W-I, Haan MM, Bear DA, Ensley SM, Yoon KJ. 2008. Survey of bovine enteric viruses in Midwestern pasture streams. Proceedings, Conference of Researcher Workers in Animal Diseases, #91. Chicago, IL; December.
Clothier, Kristin. An Investigation in to the fecal Shedding of E. coli O157:H7 from Steers on rations containing Corn Co-products, Animal Industry Report, submitted November, 2008.
Dixon PM, Yoon K-J. 2008. Estimating the mutation rate when not all mutations are detected. Proceedings, Iowa State University Fall Conference on Statistics in Biology. Ames, IA; October.
Gauger P, Opriessnig T, Pal N, Halbur PG, Lager K, and Meng XJ. Swine hepatitis E virus (HEV) infection in gnotobiotic pigs. Proc. 89th CRWAD, Chicago, Illinois, abstract #164, p 159, Dec 7-9.
Gauger PC, Lager K, Vincent AL, Opriessnig, T, Kehrli ME, Cheung AK. 2008. Experimental infections with PCV2a and PCV2b subtypes. 2008. Swine Disease Conference for Veterinarians, Ames, Iowa; November 6-7.
Hoffmann P, Prickett J, Main R, Stensland W, Yoon K-J, Sornsen S, Zimmerman J. 2008. Infectious disease surveillance in wean-finish facilities using oral fluid samples. Proceedings, Annual Meeting of American Association of Swine Veterinarians (unnumbered).
Irwin, CK 2008. Process Improvement tools for the Production Medicine Mindset. Preconference seminar in AASV Annual meeting, San Diego, CA; March 8, 2008.
Irwin, CK. 2008 Understanding pig needs and production paradigms. Eden Natural Spring 2008 newsletter, p 4-5.
Irwin, CK. 2008. Process Improvement for the Production Mind-Set, AASV Annual Conference, San Diego, CA. March.
Irwin, CK. 2008. Use of systematic reviews to inform clinical issues/decisions. ISU Swine Disease Conference proceedings. November. p123-127.
Janke B, Whitney D, Thompson C, Naberhaus N, Harmon K. 2008. Molecular diagnostic assays for swine bacterial pathogens at the Iowa State University Veterinary Diagnostic Laboratory. Proceedings, 16th Ann Swine Disease Conference for Swine Practitioners, Nov 6-7, 2008. Iowa State University, Ames, IA. pp. 29-34
Killian ML, Panigrahy B, Yoon K-J. 2008. Identification and characterization of H2N3 AIV from backyard poultry in Midwest US in comparison to novel H2N3 SIV. Proceedings, 59th North Central Avian Disease Conference. Minneapolis, MN; March.
Kim W-I, Cho Y-I, Harmon K, Madson D, Opriessnig T, Yoon K-J. 2008. Comparison of three extraction methods for the detection of PCV2 and PRRSV in semen. Proceedings, Annual Meeting of American Association of Veterinary Laboratory Diagnosticians, p138. Greensboro, NC; October.
Kim W-I, Cho Y-I, Liu S, Kinyon J, Yoon K-J. 2008. Multiplex real-time PCR test to aid the diagnosis of calf diarrhea. Proceedings, Annual Meeting of American Association of Veterinary Laboratory Diagnosticians, p69. Greensboro, NC; October.
Kim W-I, Cho Y-I, Liu S, Kinyon J, Yoon K-J. 2008. Simultaneous detection of various agents causing bovine diarrhea by multiplex real-time PCR. Proceedings, 47th Annual Meeting of North Central Conference of Veterinary Laboratory Diagnosticians, p14. Madison, WI; June.
Kuhn Michael1, Marie Gramer
2, John Johnson
3, Greg Nitzel
4, Jonathan Evans
4, Eric Wicklund
4,
Lucas Taylor4,
Jenifer Jeffers-Czach4, Vicki Rapp-Gabrielson
4. Comparison of
hemagglutination inhibition (HI) and ELISA antibody titers of pigs vaccinated with different genetic clusters of H1N1 and H3N2 swine influenza virus. Poster Leman Conference
Ma W, Lekcharoensuk P, Lager KM, Painter L, Janke BH, Vincent A, Webby R, Yoon K-J, Richt JA. 2008. The avian/human influenza viral polymerase genes enhance pathogenicity of classical swine influenza virus in mice. Proceedings, 27th Annual Meeting of American Society for Virology, Abstract W35-5. Ithaca, NY; June.
Madson D, Opriessnig T, Kuster C, Pal N, Ramamoorthy S, Meng XJ, and Halbur PG. 2008. Shedding of porcine circovirus type 2 by boars and the role of PCV-2 in semen transmission. 39th AASV, San Diego, California, p129-130, March 8-11.
Madson D, Opriessnig T, Patterson AR, Ramamoorthy S, Pal N, Halbur PG, and Meng XJ. 2008. PCV2 and semen contamination: practical implications. 16:77-80. 16th Annual Swine Disease Conference for Swine Practitioners. Ames, IA, November 6-7.
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Madson D, Patterson A, Ramamoorthy S, Pal N, Meng XJ, Opriessnig T: 2008. Reproductive effects of PCV2 in semen when used for artificial insemination. Proc 89th Annual Meeting of the Counsel of Research Workers in Animal Diseases, pp 162. Chicago, IL.
Madson D, Patterson A, Ramamoorthy S, Pal N, Meng XJ, Opriessnig T: 2008. Effect of PCV2 vaccination of dams on fetal PCV2 replication. Proc 89th Annual Meeting of the Counsel of Research Workers in Animal Diseases, pp 162. Chicago, IL.
Madson D: 2008. Posterior paresis: Differential diagnosis and interventions. Proc. 16th Swine disease conference for swine practitioners, pp. 44-46
Madson DM: 2008. A brief review of selected disease entities diagnosed in swine. 39th American Association of Swine Veterinarians, pre-conference pathology session
Madson DM: 2008. Trends in diagnostic cases: Keeping our eye on the ball. Proc. 39th American Association of Swine Veterinarians, pp 409-412
Molina RM, Cha S-H, Rowland RRR, Christopher-Hennings J, Nelson E, Lunney J, Yoon K-J, Zimmerman JJ. October 2008. Factores involucrados en la persistencia del virus de sindrome respiratorio porcino (PRRS). Memorias XXI Congreso Panamericano de Ciencias Veterinarias. pp. 533- 534. Guadalajara, México; October.
Opriessnig T, Bender J, Irwin C, and Halbur PG. 2008. Erysipelothrix rhusiopathiae: Characterization of recent outbreaks by using advanced diagnostic tools. Volume 1:137. 20th International Pig Veterinary Society Congress, Durban, South Africa, June 22-26.
Opriessnig T, Madson DM, Patterson AR, Pal N, and Halbur PG. 2008. Head-to-head comparison of the level of protection and duration of immunity induced by different commercial PCV2 vaccines. Proc. 89th CRWAD, Chicago, Illinois, abstract #143, p 156, Dec 7-9.
Opriessnig T, Madson DM, Patterson AR, Rothschild MF, Meng XJ, and Halbur P. 2008. Differences in susceptibility to PCV2-associated disease and lesions in Landrace and Pietrain pigs. 47th North Central Conference of Veterinary Laboratory Diagnosticians. Madison, Wisconsin, pp.21-23, June 5-6.
Opriessnig T, Madson DM, Patterson AR, Rothschild MF, Meng XJ, and Halbur PG. 2008. Differences in susceptibility to PCV2-associated lesions and disease in Landrace and Pietrain pigs. Volume 2:623. 20th International Pig Veterinary Society Congress, Durban, South Africa, June 22-26.
Opriessnig T, Patterson AR, Madson DM, Pal N, Rothschild M, Kuhar D, Lunney JK, Juhan NM, Meng XJ, Halbur PG: 2008. Difference in severity of porcine circovirus type 2 (PCV2)-induced pathological lesions and disease between Landrace and Pietrain pigs. Proc. 47th Annual North Central Conference of Veterinary Laboratory Diagnosticians. pp. 22-23.
Opriessnig T, Prickett JR, Madson DM, Lunney JK, Elsener J, and Halbur PG. 2008. Effect of PCV2 vaccination on PRRSV and PCV2 coinfection. 39th AASV, San Diego, California, p117-118, March 8-11.
Opriessnig T, Prickett JR, Madson DM, Lunney JK, Kuhar D, Elsener J, Halbur PG: 2008. Effect of PCV2 vaccination on PRRSV and PCV2 coinfection. Proc 20th International pig veterinary society congress, Durban, South Africa, pp. 60.
Opriessnig T, Ramamoorthy S, Madson DM, Patterson AR, Pal N, Meng XJ, Halbur PG: 2008 Experimental characterization of PCV2a and PCV2b isolates in a conventional pig model and evaluation of cross-protection between strains. Proc 39th American Association of Swine Veterinarians, pp 119-120.
Opriessnig T, Roof M, Layton SM, Madson DM, and Halbur PG. 2008. Experimental coinfection with porcine circovirus type 2 and Salmonella typhimurium or Lawsonia intracellularis. Volume 1:48. 20th International Pig Veterinary Society Congress, Durban, South Africa, June 22-26.
Opriessnig T. 2008. Current knowledge and future research needed on PCV2 vaccine. 35:63-67. Allen D. Leman Swine Conference, St Paul, Minnesota, September 22-23.
Opriessnig T. Invited interview on “More to learn about porcine circovirus”. 2008. National Hog Farmer 53:14-15.
Opriessnig T. PCVD – From pathogenesis to protection; Part 5. PCVD and associated disease. 2008. Pig Progress 24:24-26.
Osweiler GD, Survey of Recent Food Animal Toxicoses from Contaminated Feed. American Association of Veterinary Laboratory Diagnosticians, Greensboro, NC, October 26, 2008
Patterson Abby, John Johnson, Sheela Ramamoorthy, Xiang-Jin Meng, Patrick G. Halbur, Tanja Opriessnig. Comparison of the ability of three PCV2 ELISAs to detect PCV2-specific antibodies after PCV2 vaccination or inoculation with distinct PCV1 or PCV2 isolate
46
Patterson AR, and Opriessnig T. 2008. Interlaboratory comparison of serological assays for porcine circovirus type 2. 47th North Central Conference of Veterinary Laboratory Diagnosticians. Madison, Wisconsin, pp.25-26, June 5-6.
Patterson AR, and Opriessnig T. 2008. Interlaboratory comparison of serological assays for porcine circovirus type 2. 51:176. AAVLD 51th Annual Conference, Greensboro, North Carolina, October 22-27.
Patterson AR, Cooper V, Yoon K-J. 2008. Naturally occurring influenza infection in a ferret (Mustela putorius furo) colony. Proceedings, Annual Meeting of American Association of Veterinary Laboratory Diagnosticians, p101. Greensboro, NC; October.
Pittman Jeremy S1, DVM; Brad J Thacker
2, DVM, PhD, DABVP; John K Johnson
3, DVM.
Circumvent® PCV Vaccination Implementation and Compliance in a Large Production System. 1) Murphy-Brown, LLC–Waverly Division, Waverly, Virginia. 2) Intervet/Schering-Plough Animal Health, Desoto, Kansas. 3) Veterinary Diagnostic Laboratory, Iowa State University, Ames, Iowa.
Prickett J, Hoffmann P, Stensland W, Yoon K-J, Zimmerman J. 2008. Practical disease surveillance in growing pig populations. Proceedings, Annual Meeting of American Association of Veterinary Laboratory Diagnosticians, p139. Greensboro, NC; October.
Prickett JR, Cutler S, Kinyon J, Naberhaus N, Stensland WR, Yoon K-J, Zimmerman JJ. 2008. PRRSV surveillance – stability of diagnostic targets in oral fluid: sample storage and critical techniques for testing. Proceedings, Conference of Researcher Workers in Animal Diseases, #38. Chicago, IL; December.
Prickett JR, Cutler S, Kinyon J, Naberhaus N, Stensland WR, Yoon K-J, Zimmerman JJ. 2008. PRRSV surveillance – stability of diagnostic targets in oral fluid: sample storage and critical techniques for testing. Proceedings, International PRRS Symposium, p26. Chicago, IL; December.
Prickett JR, Cutler S, Kinyon J, Naberhaus N, Stensland WR, Yoon K-J, Zimmerman JJ. 2008. PRRSV surveillance – stability of diagnostic targets in oral fluid: sample storage and critical techniques for testing. Proceedings, Annual Meeting of American Association of Veterinary Laboratory Diagnosticians, p140. Greensboro, NC; October.
Prickett JR, Cutler S, Kinyon J, Naberhaus N, Stensland WR, Yoon K-J, Zimmerman JJ. 2008. PRRS surveillance – Stability of diagnostic targets in oral fluid: Sample storage and critical techniques for testing. Proceedings, 47th Annual Meeting of North Central Conference of Veterinary Laboratory Diagnosticians, pp 10-11. Madison, WI; June.
Prickett JR, Hoffmann P, Main R, Stensland W, Yoon K-J, Zimmerman J. 2008. Practical disease surveillance in growing pig populations. Proceedings, Conference of Researcher Workers in Animal Diseases, #39. Chicago, IL; December.
Prickett JR, Hoffmann P, Main R, Stensland W, Yoon K-J, Zimmerman J. 2008. Practical disease surveillance in growing pig populations. Proceedings, International PRRS Symposium, p27. Chicago, IL; December.
Prickett JR, Hoffmann P, Main R, Stensland W, Yoon K-J, Zimmerman JJ. 2008. Practical disease surveillance in growing pig populations. Proceedings, 47th Annual Meeting of North Central Conference of Veterinary Laboratory Diagnosticians, pp 8-9. Madison, WI; June.
Russell JR, Ensley SM, Yoon K-J. 2008. Grazing management effects on pathogen loading of midwestern pasture streams. Proceedings, USDA-CSREES National Water Conference (unnumbered). Washington DC; February.
Schwartz, Kent 2008: Leman Conference 2008: AASV Annual Meeting 2008: IVMA Winter Conference 2008: Pfizer 2008: IPVS, South Africa (2 presentations/abstracts) 2008: Australian Pig Veterinary Society 2008: BIVI VPP (1/2 day workshop with materials) 2008: BIVI Pork Academy (1 week workshop with materials) 2008: NCCVLD: Data mining and applications 2008: NCVLM: Data mining and applications
Sun D, Kim W-I, Cho Y-I, Cha S-H, Kim S-H, Choi E-J, Yoon KJ. 2008. Cross-protection induced by chimeric mutant containing mixed structural genes of two different PRRS viruses.
47
Proceedings, Conference of Researcher Workers in Animal Diseases, #171. Chicago, IL; December.
Sun D, Kim W-I, Cho Y-I, Yoon KJ. 2008. Attempt to achieve broader cross-protection among PRRS viruses by vaccination: use of chimeric mutant containing mixed structural genes of two different PRRS viruses. Proceedings, International PRRS Symposium, p80. Chicago, IL; December.
Thacker B, W Wilson, J Johnson, C Francisco, R Schlueter. CircumventTM PCV vaccine: Performance and Serological response evaluation. Intervet Inc., Millsboro, Delaware. Iowa State University Veterinary Diagnostic Laboratory, Ames, Iowa.
Thacker Brad1, Rich Schlueter
1, John Johnson
2. Utility of an Indirect ELISA for Circumvent® PCV
Compliance Monitoring. 1) Intervet/Schering-Plough Animal Health, Desoto, Kansas. 2) Iowa State University Veterinary Diagnostic Laboratory, Ames, Iowa
Vincent A, Lager KM, Richt JA, Ma W, Gauger PC, Janke BH, Gramer MR. Swine influenza virus: Strategies to control a moving target. Proceedings, Winter Meeting, Iowa Veterinary Medical Association, Iowa State University, Ames, IA. Feb 6-7, 2008. pp. 239-246.
Vu HLX, M. Brito M, Kim WI, Yoon KJ, Laegreid W, Osorio FA. 2008. Sub-typing PRRSV isolates by means of measurement of cross neutralization reactions. Proceedings, Conference of Researcher Workers in Animal Diseases #88P. Chicago, IL; December.
Vu HLX, M. Brito M, Kim WI, Yoon KJ, Laegreid W, Osorio FA. 2008. Sub-typing PRRSV isolates by means of measurement of cross neutralization reactions. Proceedings, International PRRS Symposium, p74. Chicago, IL; December.
Yoon K-J. 2008. PRRS diagnosis as tool for evidence-based PRRS control. Proceedings, Annual Meeting of Korean Association of Swine Veterinarians. pp 20-29. Icheon, Korea; March.
Zimmerman J, Prickett J, Molina R, Hoffmann P, Main R, Sornsen S, Johnson J. October 14, 2008. Medicina en producción porcina II: Un enfoque a la ecología-epidemiología de las enfermedades. XXI Congreso Panamericano de Ciencias Veterinarias. Guadalajara, México.
Zimmerman J, Prickett J, Molina R, Hoffmann P, Main R, Sornsen S, Johnson J. October 10, 2008. Control de enfermedades en poblaciones porcinas: Perfiles prognósticos usando muestras de fluidos orales. Simposium - Problemas en la producción intensive del cerdo salud pública. Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México. Ciudad Universitaria, D.F.
Invited Presentations Clothier, Kris. Johne’s Disease and Diagnostic Medicine Presentation to South West Iowa
Veterinary Medical Association, April, 2008. Clothier, Kris. Johne’s Disease and Diagnostic Medicine Presentations to North West Iowa
Extension Field Day, March, 2008. Clothier, Kris. NAHLN Emergency Management Symposium, October, 2008, presented summary
of NAHLN HPAI Table Top Exercise Game, information on NAHLN Virtual Library Clothier, Kris. NCCVLD Presentation on “Accessory Thymic tissue in a juvenile goat”, June, 2008. Clothier, Kris. Zoonotic Diseases and opportunities for collaboration between MD’s and DVM’s,
Drake University Public Health Medicine Group, October, 2008. Ensley, Steve. Diagnostic Investigation of Health Issues Associated with High Sulfur Feeds. High
Plains BioFuel Meeting, Plaza Hotel, Garden City Kansas. 1/20/08, 1 hour presentation. Pg 1-13.
Ensley, Steve. Diagnostic Investigation of Suspect Pet Food Toxicosis. AASP annual conference, San Diego. 3/8/08, 1 hour presentation. Preconference workshop.
Ensley, Steve. Mycotoxins in alternate feeds. IVMA winter conference, 2/7/08, 1 hour presentation. Ensley, Steve. Update in the ISU VDL. North Central IVMA, Ft Dodge, Iowa. 5/13/08, 1 hour
presentation. Gauger PC, Lager K, Vincent AL, Opriessnig, T., Kehrli ME, Cheung AK. 2008. Experimental
infections with PCV2a and PCV2b subtypes. 2008. Swine Disease Conference for Veterinarians, Ames, Iowa; November 6-7.
Halbur P, T Opriessnig, E Strait. Respiratory Disease Pathology Update. 2008. Boehringer Ingelheim Vetmedica, Swine Veterinarian Academy; Sept. 15.
48
Irwin, Christa. “Holistic approach to swine production”, Niman Ranch Production Management Meeting, Ames, Iowa, November 20, 2008.
Irwin, Christa. “Process Improvement for the Production Mind-Set”, AASV annual meeting, San Diego, CA, March 8-11, 2008.
Irwin, Christa. “Understanding pig needs and production paradigms”, Eden Natural Annual Business Meeting, Marshalltown, IA, February 2008.
Irwin, Christa. “Use of systematic reviews to inform clinical issues/decisions”, ISU Swine Disease Conference, Ames Iowa, November 6-7, 2008.
Madson, Darin, Fort Dodge Animal Heath, Swine Veterinarian Workshop, Clear Lake, Iowa (5-22-08) – ISU Veterinary Diagnostic Laboratory Update: Current Trends.
Madson, Darin, Pfizer Animal Health, 13th Annual Galena Swine Veterinary Seminar, Galena, IL (6-11-08) – Current understanding of PCV2 and related research.
Madson, Darin, Pipestone System and Pfizer Animal Health Care Clinic, Sioux Falls, SD (3-28-08) – Necropsy Story: What the pigs telling you.
Opriessnig, Tanja, Challenges and success in diagnosing and controlling PRRSV in the United States. Intervet Germany, Practitioner meeting. Reykjavik, Iceland, April 26-27, 2008.
Opriessnig, Tanja, Current knowledge and future research needed on PCV2 vaccine. Allen D. Leman Swine Conference, September 22-23 2008, St Paul, Minnesota, 35:63-67.
Opriessnig, Tanja, PCV2 and reproductive failure, Norson producer and staff veterinarian meeting, Hermosillo, Mexico, January 7, 2009.
Opriessnig, Tanja, PCV2 e PCVD nell’allevamento suino americano: esperienze diagnostiche e di controllo (porcine circovirus associated disease: diagnosis and control)”. XXXIII corso in patologia suina e tecnica dell’allevamento, Fondazione iniziative zooprofilattiche e zootecniche Brescia, Brescia, Italy, November 18, 2008.
Opriessnig, Tanja, PCVAD disease and control. Swine Academy, Boehringer Ingelheim Vetmedica Inc., Ames, Iowa, USA, September 16, 2008.
Opriessnig, Tanja, Stand der Erforschung und Bekämpfung der PCV-2-Infektion (Porcine Circovirus Associated Disease - PCVAD). 38:36-50. 38. Tagung des Arbeitskreises Tiergarten, Tierärztliche Fortbildung für Schweinegesundheit und Schweineproduktion. Hannover, Germany, May 23-24, 2008.
Opriessnig, Tanja, Update on PCV2 vaccination, Smithfield staff veterinarian meeting, Mexico, January 6, 2009.
Osweiler G.D., Four presentations, one hour each: (1) Diagnostic toxicology for equine practice, (2) Continuing threats in equine toxicology, (3) Common equine drug toxicities and adverse reactions, and (4) Poisonous plants and natural toxins: threats to equine health. Georgia Veterinary Academy Annual Meeting, Sandestin, FL, May 30, 2008.
Osweiler G.D., Survey of Feed Contamination Incidents Causing Animal Poisoning, Center for Veterinary Medicine – FDA, Rockville, MD, February 8, 2008.
Osweiler G.D., Survey of Feed Contamination Incidents Causing Animal Poisoning, American Association of Feed Control Officials, Nashville, TN, August 2, 2008
Prickett JR, Hoffmann P, Main R, Stensland W, Yoon K-J, Zimmerman J. December 2008. Practical disease surveillance in growing pig populations. International PRRS Symposium. Chicago, Illinois.
Russell JR, Ensley SM, Yoon K-J. February 2008. Grazing management effects on pathogen loading of midwestern pasture streams. USDA-CSREES National Water Conference. Washington DC.
Schwartz, Kent. 2008 AASV: Tools for better evidence workshop Schwartz, Kent. 2008 AASV: Grow finish disease Schwartz, Kent. 2008: AASV Annual Meeting Schwartz, Kent. 2008: Australian Pig Veterinary Society Schwartz, Kent. 2008: BIVI Pork Academy (1 week workshop with materials) Schwartz, Kent. 2008: BIVI VPP (1/2 day workshop with materials) Schwartz, Kent. 2008: IPVS, South Africa (2 presentations/abstracts) Schwartz, Kent. 2008: IVMA Winter Conference Schwartz, Kent. 2008: IVMA: Actinobacillus suis Schwartz, Kent. 2008: Leman Conference Schwartz, Kent. 2008: NCVLD: Data mining and applications Schwartz, Kent. 2008: NCVLM: Data mining and applications
49
Schwartz, Kent. 2008: Pfizer Schwartz, Kent. 2008: Walking the Pens Workshop, Charlotte, NC. Strait Erin. Update on Molecular Bacteriology Testing. 2008. Expanding Tribal Knowledge (ETK)
Departmental seminar; Nov 13. Strait Erin. Update on Mycoplasma Diagnostics for Swine. 2008. Swine Vet Center, St. Peter, MN;
Oct. 17. Yoon KJ. December 2008. Viral recombination among field isolates of PRRSV type 2 – implication
for molecular epidemiology. International PRRS Symposium. Chicago, Illinois. Yoon K-J. March 2008. PRRS diagnosis as tool for evidence-based PRRS control. Annual
Meeting of Korean Association of Swine Veterinarians, Suwon, Korea. Yoon K-J. March 2008. Research update in PRRSV and PCV2. ChoongAng Vaccine Co,
Daejeon, Korea. Yoon K-J. March 2008. Recent trends of swine diseases in US: Midst of microorganisms Annual
Meeting of Korean Association of Swine Veterinarians, Suwon, Korea. Yoon K-J. October 2008. Canine influenza outbreak and surveillance in Iowa. Animal Health
Insitute Veterinary Biologics Meeting, Ames, Iowa. Other presentations including abstracts Beckler Dana; Joann Kinyon; Brad Gramm; Carbadox MIC values against Salmonella spp. isolated
from swine; AASV, March 08 Bender, JS, J Kinyon, C Irwin, KJ Schwartz, T Opriessnig. Erysipelothrix rhusiopathiae: Comparison
of diagnostic tools and characterization of recent outbreaks. In: North Central Conference of Veterinary Laboratory Diagnosticians, Madison, WI. 2008. pp.40-42 Outstanding Graduate Presentation Award.
Gauger P, Lager KM, Vincent AL, Opriessnig T, Kehrli Jr ME, and Cheung AK. 2008. Pathogenesis of PCV2a and PCV2b virus in germ-free pigs. Volume 1:28. 20th International Pig Veterinary Society Congress, Durban, South Africa, June 22-26.
Gauger P, Lager KM, Vincent AL, Opriessnig T, Kehrli ME, and Cheung AK. 2008. Experimental infections with PCV2a and PCV2b subtypes. 16:69-76. 16th Annual Swine Disease Conference for Swine Practitioners. Ames, IA, November 6-7.
Irwin CK, O’Connor AM. Analysis of screening questions for systematic reviews – how do “can’t tell” responses resolve? The Canadian Association of Veterinary Epidemiology Preventive Medicine conference, Prince Edward Island, CA, May 21-30, 2008
Irwin CK. Systematic review of swine euthanasia methods and welfare measures reported. Welfare & Epidemiology Conference, Ames, Iowa, July 14-16, 2008.
Jarvinen, JA, and Kinyon, JM, Preputial microflora of llamas (Lama glama) and alpacas (Vicugna pacos); Draft prepared; plan to submit to JDiagInvest
Kim W, Cho Y, Harmon K, Madson D, Opriessnig T, and Yoon KJ. 2008. Comparison of three extraction methods for the detection of PCV2 and PRRSV in semen. 51:138. AAVLD 51th Annual Conference, Greensboro, North Carolina, October 22-27.
Kind, AJ and W Hyde, “Characterization of seized cobratoxin preparations by mass spectrometry”. Presentation 17th International Conference of Racing Analysts and Veterinarians, Antalya, Turkey, R & W Communications, 2008.
Madson DM, Patterson AR, Ramamoorthy S, Pal N, Meng XJ, Opriessnig T: 2008. Effect of PCV2 vaccination of dams on fetal PCV2 replication. Proc. 89th Conference of Research Worker in Animal Disease, pp. 162.
Opriessnig T, JS Bender, J Kinyon, KJ Schwartz, C Irwin, P Halbur. Erysipelothrix rhusiopathiae: Characterization of recent outbreaks by using advanced diagnostic tools. In: 20th International Pig Veterinary Society Congress, Durban, South Africa.2008. pp. 137.
Opriessnig T, Madson DM, Patterson AR, Pal N, Halbur PG: 2008. Head-to-head comparison of the level of protection and duration of immunity induced by different commercial PCV2 vaccines. Proc. 89th Conference of Research Worker in Animal Disease, pp. 156.
Osweiler G.D., SM Ensley, S Jagannatha, DW Trampel, PM Imerman, KJ Yoon, DT Moore. Evaluation of Novel Aflatoxin Protective Additives for Poultry. Poultry Science Association, Niagara Falls, Ontario, Canada, July 22, 2008.
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Prickett JR, Cutler S, Kinyon J, Naberhaus N, Stensland W, Yoon KJ, Zimmerman J. October 26, 2008. PRRSV surveillance – Stability of diagnostic targets in oral fluid: sample storage and critical techniques for testing. Presented, Best Graduate Student Presentation, Proceedings of the 51st American Association of Veterinary Laboratory Diagnosticians. Greensboro, North Carolina.
Strait E, M Madsen, FC Minion, J Christopher-Hennings, M Dammen, and E Thacker*. 2008 Development of two sensitive Mycoplasma hyopneumoniae-specific real-time PCR assays. 20th International Pig Veterinary Society Congress, Durbin, South Africa; June 22-26
Strait EL, ML Madsen, FC Minion, J Christopher-Hennings, M Dammen, and EL Thacker. 2008. Development of Two Sensitive Mycoplasma hyopneumoniae-Specific Real-Time PCR Assays. International Organization of Mycoplasmologists (IOM),Tianjin, China; July 4-12
Yong-Il Cho, Won-Il Kim, Siyuan Liu, Joann Kinyon, Kyoung-Jin Yoon, Development of Multiplex Real-time PCR for Simultaneous Detection of Various Agents Causing Bovine Diarrhea; Presented at AAVLD; paper in progress.
Young G, Irwin CK. A systematic review of CO2 euthanasia in swine. AASV Annual meeting student presentations. 2009. poster session.
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Glossary
AgELISA Antigen-capture ELISA AGG Agglutination AGID Agar gel immunodiffusion AIV Avian influenza virus ALA Automated latex agglutination BAPA Buffered acidified plate antigen test BRSV Bovine respiratory syncytial virus BSE Bovine spongiform encephalopathy BVDV Bovine viral diarrhea virus cELISA Competitive (blocking) ELISA CF Complement fixation CSF Classical Swine Fever EDTA Ethylenediaminetetraacetate ELISA Enzyme-linked immunosorbent assay EM Electron microscopy FA Fluorescent antibody FAD Foreign Animal Diseases FADDL Foreign Animal Disease Diagnostic Laboratory (USDA) FATS Fluorescent antibody tissue section FFN Fluorescent focus neutralization FSVS Food Supply Veterinary Services HI Hemagglutination inhibition IBT Immunoblot assay IFA Indirect fluorescent antibody IHC Immunohistochemistry IPT Immunoperoxidase test LAT Latex agglutination Mab Monoclonal antibody MAT Microscopic agglutination test NADC National Animal Disease Center (USDA:ARS) NVSL National Veterinary Services Laboratories (USDA:APHIS) PAM Production Animal Medicine, Iowa State University PCFIA Particle concentration fluorescence immunoassay PCR Polymerase chain reaction PCV Porcine circovirus PI Persistent infection PPV Porcine parvovirus PRCV Porcine respiratory coronavirus PRRS virus Porcine reproductive and respiratory syndrome virus PRV Pseudorabies (Aujeszky’s disease) virus RAP Rapid automated presumptive test RFLP Restriction fragment length polymorphism RIV Rivanol precipitation – plate agglutination test RSAT Rapid slide agglutination test SIV Swine influenza virus SPT Standard plate agglutination test STT Standard tube agglutination test TGEV Transmissible gastroenteritis virus VDL Veterinary Diagnostic Laboratory, Iowa State University VDPAM Department of Veterinary Diagnostic and Production Animal Medicine VI Virus isolation VME Veterinary Medicine Extension, Iowa State University VMRI Veterinary Medical Research Institute, Iowa State University VN Serum-virus neutralization