Systems Biology approaches tounderstanding glutathione metabolism

29 September 2010

Dr Najl Valeyev

Edinburgh

University of Kent

Centre for Molecular Processing

WeatherElectronic circuits design

Aircraft flight controllers Stock market

Different types of Systems

Causative knockouts of “functional” parts

Lazebnik Y, Cancer Cell. 2002 Sep;2(3):179-82

Linking parts with “disease” phenotypesLinking parts with “disease” phenotypes

Family classification according to Phenotype

Family classification according to Phenotype

Idealised Cell as a System

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The Complexity Problem

Science or Art ?

The Complexity Problem

Major Modelling Principle

TrueObject

Knowledge 1 Knowledge 2

Knowledge 3 Knowledge 4

TrueObject

Model 1 Model 2

Model 3 Model 4

wt

gshA-

speA

-

gshA-sp

eA-

speE

- KCEC

1777

gshA-sp

eE- K

CEC2714

prpD- K

CEC2722

0.0

2.0

4.0

6.0

8.0

10.0

12.0

Comparison of experiments with simulations

Experiments Model predictions

wt

gshA

-

gshA

-Spe

A-

SpeA

-

Stochastic interactions between models and experiments

should lead to deterministic conclusions

to test and evaluate the structure of the assumed intracellular interactions with the pathways

to identify inconsistent hypotheses and design experiments to clarify the properties of signalling pathways

to provide advanced interpretation of the experimental data

to developed an integrative model of fimbriation