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  • Supplementary - Final 12-05-17 · 2017. 6. 28. · 3 Supplementary Fig.S-1: On chip diaphragm pump geometry Supplementary Fig.S-1: On chip diaphragm pump geometry.Fig.S-1 A shows
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1 Supplementary Material: An automated optofluidic biosensor platform combining interferometric sensors and injection moulded microfluidics C. Szydzik 1 , A.F. Gavela 2 , S. Herranz 2 , J. Roccisano 1 , M. Knoerzer 1 , P. Thurgood 1 , K. Khoshmanesh 1 , A. Mitchell 1 , L.M. Lechuga* 2 1 School of Engineering, RMIT University, Melbourne Australia 2 Nanobiosensors and Bioanalytical Applications Group. Catalan Institute of Nanoscience and Nanotechnology (ICN2). CSIC, CIBER-BBN and The Barcelona Institute of Science and Technology, Campus UAB, Bellaterra, 08193 Barcelona, Spain. * Corresponding authors: [email protected] Electronic Supplementary Material (ESI) for Lab on a Chip. This journal is © The Royal Society of Chemistry 2017

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Page 1: Supplementary - Final 12-05-17 · 2017. 6. 28. · 3 Supplementary Fig.S-1: On chip diaphragm pump geometry Supplementary Fig.S-1: On chip diaphragm pump geometry.Fig.S-1 A shows

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SupplementaryMaterial:

Anautomatedoptofluidicbiosensorplatformcombininginterferometricsensorsandinjectionmoulded

microfluidics

C.Szydzik1,A.F.Gavela2,S.Herranz2,J.Roccisano1,M.Knoerzer1,P.Thurgood1,K.Khoshmanesh1,A.Mitchell1,L.M.Lechuga*2

1SchoolofEngineering,RMITUniversity,MelbourneAustralia2NanobiosensorsandBioanalyticalApplicationsGroup.CatalanInstituteofNanoscienceandNanotechnology(ICN2).CSIC,CIBER-BBNandTheBarcelonaInstituteofScienceandTechnology,CampusUAB,Bellaterra,08193Barcelona,Spain.

*Correspondingauthors:[email protected]

Electronic Supplementary Material (ESI) for Lab on a Chip.This journal is © The Royal Society of Chemistry 2017

Page 2: Supplementary - Final 12-05-17 · 2017. 6. 28. · 3 Supplementary Fig.S-1: On chip diaphragm pump geometry Supplementary Fig.S-1: On chip diaphragm pump geometry.Fig.S-1 A shows

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SupplementaryMaterialSM-1:ExperimentalsetupOperation of the Bimodal waveguide biosensor was conducted using an experimental setup similar topreviouslyoutlined1.AHe-Nelaser(632.8nm,10mW)wasend-firecoupledtothebiosensorthrougha40xmicroscopeobjective usingTEpolarization, the resultant interferometric signalwasacquiredwith aduelsegmentphotodiode(S5870,Hamamtsu)anddataanalysiswasperformedusingLabVIEWsoftware(NationalInstruments,USA).Themicrofluidicmodulewasclamped to theBiMWsensordirectlybetweenaPMMAclampandathermalregulationstage,stabilisingintrinsicsignaldriftduetoenvironmentalthermalinfluence

Page 3: Supplementary - Final 12-05-17 · 2017. 6. 28. · 3 Supplementary Fig.S-1: On chip diaphragm pump geometry Supplementary Fig.S-1: On chip diaphragm pump geometry.Fig.S-1 A shows

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SupplementaryFig.S-1:Onchipdiaphragmpumpgeometry

SupplementaryFig.S-1:Onchipdiaphragmpumpgeometry.Fig.S-1Ashowsadissectedsegmentofthemicrofluidicmodule,showingthevariouscriticalcomponents,andillustratingtheirrelativesize.Adashedline through thepump chambershowsacut,withFig.S-1B showingacross sectional imageof this cutsurface,illustratingtherelativethicknessofprimary,membrane,andactuationchambercomponentsofthevalvesusedinthismodule.

Page 4: Supplementary - Final 12-05-17 · 2017. 6. 28. · 3 Supplementary Fig.S-1: On chip diaphragm pump geometry Supplementary Fig.S-1: On chip diaphragm pump geometry.Fig.S-1 A shows

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SupplementaryFig.S-2:Rawsensordata

Supplementary Fig.S-2: Raw sensor data for reference andcompetitiveassaypairs.sensorsignalandextrapolatedsignalinπradareshowninredatthetopofeachgraph

Page 5: Supplementary - Final 12-05-17 · 2017. 6. 28. · 3 Supplementary Fig.S-1: On chip diaphragm pump geometry Supplementary Fig.S-1: On chip diaphragm pump geometry.Fig.S-1 A shows

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REFERENCES

1. K. E. Zinoviev, A. B. González-Guerrero, C. Domínguez and L. M. Lechuga, Journal of Lightwave Technology 29 (13), 1926-1930 (2011).


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