SUPPLEMENTARY FIGURES

TGF-RI

TGF-RII

GAPDH

KLE Hec-1-A Hela

C.HIESC

Supp. 1

Par-4

-actin

Par-4

-actin

A.

B.SKOV-3

TGF-3TGF-2TGF-1

---

--+

-+-

+--

MCF7

TGF-3TGF-2TGF-1

---

--+

-+-

+--

SKOV-3TGF-3TGF-2TGF-1

---

--+

-+-

+--

MCF7

TGF-3TGF-2TGF-1

---

--+

-+-

+--

*** ***

*

* ***

SKOV-3 MCF7

Par-4

P-Smad2

GAPDH

Alk5 inh. - +

A

B Hiesc

E-cadherin

N-cadherin

Snail

β-actin

KLE

E-cadherin

N-cadherin

Snail

β-actin

Hec-1-A

C

Days 0 3 6

GAPDH

Snail

N-Cadherin

Vimentin

Days 0 3 6

TGF-3

Par

-4/G

AP

DH

GAPDH

Par-4

543210

D

Supp. 2

E

SKOV-3

E-cadherin

N-cadherin

Snail

β-actin

MCF7TGF-3TGF-2TGF-1

---

--+

-+-

+--

TGF-3TGF-2TGF-1

---

--+

-+-

+--

TGF-3TGF-2TGF-1

---

--+

-+-

+--

TGF-3TGF-2TGF-1

---

--+

-+-

+--

Claudin-1

β-actin

KLE SKOV-3

TG

F-

3C

ontr

ol

C N C N C N C N

Par-4

p-Smad2

Control TGF-1 TGF-2 TGF-3

GAPDH

PARP

Supp. 3

Par-4 Hoechst Merge

Cont

rol

TGF-

1TG

F-2

TGF-

3B.

C.A Empty

Par-4

E-cadherin

Myc-Par-4

Vimentin

Snail

β-actin

-+

+- Empty

Par-4

Myc-Par-4

Claudin-1

β-actin

-+

+-

SKOV-3 MCF7

TGFβTGFβ

NucleusNucleus

p110p110

p85p85

PIP3PIP3

PP

PPPTENPTEN

AktAkt

PDK1PDK1

PP

PP

Ser473Ser473

Thr308Thr308

PI3K

PI3K

PIP2PIP2

MitochondriaMitochondria

Cytochrome CCytochrome C

Apaf-1Apaf-1

BakBak BaxBax

BadBadPP BadBad

Bcl-2Bcl-2

Par-4Par-4

ApoptosisApoptosis

UbUb

Smad2/3Smad2/3

TGFR

TGFR

TGFR

TGFR

Par-4Par-4

Smad2/3Smad2/3

EMTEMT

IKKIKK

p50

p50

p65

p65

IkBIkB

p50

p50

p65

p65

IkBIkB

PP

C9C9 XIAPXIAP

C3C3

TGFβTGFβ

TGFRTGFRTGFR

TGFR

cleavagecleavage

Par-4Par-4

XIAPXIAP

Smad4Smad4

PP

PP

XIAPXIAP

Supp. 4