FZD7 Supplementary figures

Supplementary Figure 1

SKOV3-P2D Parental

SKOV3-S3D Spheroid

SKOV3-S2D Spheroid-reattached

A)

B)

CH1

FZD7-5 siRNA FZD7-6 siRNA NEG. CONTROL

PI

Annexin-V

PA-1

OV-17R

Supplementary Figure 2

A)

ANNEXIN V

Supplementary Figure 2

B)

C)

D)

Supplementary Figure 3A) B)

C)

D)

FZD7-6NEG. CONTROL

F-actin-cat Merge

F-actin-cat

FZD7-5

Merge

MergeF-actin-cat

CH1

OV-17R

PA-1

Supplementary Figure 4

FZD7-5 FZD7-6 NEG. CONTROL

CH1

OV-17R

PA-1

Supplementary Figure 5

CDC42

Supplementary Figure 6

FZD7-6 siRNA NEG. CONTROLFZD7-5 siRNA

0-hrs

15-hrs

Supplementary Figure 7

A) CH1

FZD7-6 siRNA NEG. CONTROLFZD7-5 siRNA

0-hrs

15-hrs

Supplementary Figure

B) PA-1

FZD7-6 siRNA NEG. CONTROLFZD7-5 siRNA

0-hrs

32-hrs

Supplementary Figure

C) OV-17R

NEG.CONTROL

FZD7-5

FZD7-6

γ-tubulinCk1ε

γ-tubulinCk1ε

γ-tubulinCk1ε

γ-tubulinDVL2 pSer143

γ-tubulinDVL2 pSer143

γ-tubulinDVL2 pSer143

Merge

Merge

Merge

Merge

Merge

Merge

A) CH1

Supplementary Figure 8

γ-tubulinCk1ε

γ-tubulinCk1ε

γ-tubulinCk1ε

γ-tubulinDVL2 pSer143

γ-tubulinDVL2 pSer143

γ-tubulinDVL2 pSer143

Merge

Merge

Merge

Merge

Merge

Merge

NEG.CONTROL

FZD7-5

FZD7-6

B) PA-1

Supplementary Figure 8

γ-tubulinCk1ε

γ-tubulinCk1ε

γ-tubulinCk1ε

γ-tubulinDVL2 pSer143

γ-tubulinDVL2 pSer143

γ-tubulinDVL2 pSer143

Merge

Merge

Merge

Merge

Merge

Merge

NEG.CONTROL

FZD7-5

FZD7-6

C) OV-17RSupplementary Figure 8

FZD7-6 siRNAFZD7-5 siRNA+Wnt5a +Wnt5a

-Wnt5a -Wnt5a

Supplementary Figure 9FZD7-6 siRNAFZD7-5 siRNA

+Wnt5a +Wnt5a

-Wnt5a -Wnt5a

+Wnt5a +Wnt5a

-Wnt5a -Wnt5a

A)CH1 B)PA1

C)OV-17R