study of asymptomatic bacteriuria and sensitivity pattern of

8/8/2019 Study of Asymptomatic Bacteriuria and Sensitivity Pattern Of

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JYOTSNA

BBT FINAL YEAR


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DEFINITION

Presence of bacteria >105 CFU/ml of

urine of a single pathogen in two

consecutive midstream, clean-catch

urine specimen without symptoms of

urinary tract infection.


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CAUSES

According to Williams, progesterone

causes atonia of smooth muscles during

pregnancy. If the smooth muscles of the

genitourinary tract are affected, atonia

will lead to stasis of the urinary out flow

and infection would follow.

Incidence also depends on age, parity,race and socio-economic status.

pH of urine


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1)To detect asymptomatic bacteriuria in pregnantwomen attending gynecology OPD of Patna

Medical College and Hospital, Patna .

2)Isolation and identification of the causative

microorganism of asymptomatic bacteriuria.3)To find out the prevalence with respect to age,

parity and socio-economic status of the

pregnant women.

4)To study the sensitivity pattern of the

microorganism against different antimicrobials.


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PLACE OF WORK

Department of microbiology, Patna

Medical College.

The present study comprised of 50cases attending the Medical College.


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Urine of the asymptomatic pregnant

women

Cultured on Blood agar, Mac Conkey

agar and Nutrient agar

Colony counting and characterization isdone after incubation

>105CFU/ml of urine Significant growth

103

-105

CFU/ml of urine Growth of doubtful significance


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E. COLIONMAC CONKEYAGAR

E.COLION BLOOD AGAR


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KLEBSIELLA PNEUMONIA

ON MAC CONKEY AGARKLEBSIELLA PNEUMONIA

ON BLOOD AGAR


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PROTEUS MIRABILIS ON

BLOOD AGAR PLATE


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PSEUDOMONASAERUGINOSA ON MACCONKEY AGAR

PSEUDOMONASAERUGINOSA ONNUTRIENTAGAR


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DIFFERENT BIOCHEMICAL

TESTS FOR IDENTIFICATION

OF MICRO-ORGANISM


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CYTOCHROME OXIDASE TEST

1% solution of

tetramethyl-p-phenylene

diamine hydrochloride

Oxidase positive

Pseudomonadaceae

Oxidase negativeEnterobacteriaceae


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CATALASE TEST

3% hydrogen peroxide

Catalase positive

Enterobacteriaceae

Pseudomonas


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INDOLE TEST

0.5ml Kovacs reagent

Indole positive(red color)

E.coli


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METHYLRED TEST

5 drops of Methyl red

reagent

Positive result(red color)

E.coli

Negative result(yellow

color)other


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CITRATE TEST

Simmons citrate medium

is used

Citrate positive(blue color)

Klebsiella pneumonia

Proteusmirabilis

Citrate negative(no colorchange)

E.coli

Psedomonas aeruginosa


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UREASE TEST

Christensens urease

media is used

Positive (pink red color)

Proteusmirabilis

Klebsiellapneumonia

Negative(no color changeor change to pale yellow)

E.coli


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ANTIBIOTICSUSCEPTIBILITY

TESTING 3-5 well isolated colonies were touched

and emulsified in sterile saline.

Turbidity of suspension was matched toturbidity standard.

Using a sterile swab ,inoculation was doneon Mueller Hinton agar plate.

Antibiotic disc were evenly distributed onthe inoculated plate.

The plates were incubated overnight at37C .

The reading was taken on the next day.


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RESULTS

AND

OBSERVATION


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INCIDENCE OF ASYMPTOMATIC

BACTERIURIA


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ISOLATED ORGANISM IN BACTERIURIA

CASES


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CASES IN DIFFERENTAGE GROUPS


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PARITYWISE DISTRIBUTION


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SOCIO ECONOMICSTATUS


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SENSITIVITY TO DIFFERENTANTIBIOTIC


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RESISTANCE TO DIFFERENTANTIMICROBIAL


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CONCLUSION


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The incidence of asymptomatic bacteriuria in pregnancy was found to be

10%.

The age of patients ranged from 15-45 years, the highest percentage ofbacteriuric patient were from 15-25 yrs.

The incidence of asymptomatic bacteriuria was found to be increasing with

decreasing socio-economic status.

Among the infecting organism,

Escherichia coliwas taken to be standard

Cases were sensitive to Erythromycin,Norfloxacin

Cases were resistance toAmpicilin,Amoxycillin

If untreated and undetected,it may lead to acute or chronic infection and

eventual death.


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REFERENCES

1. Abbott, G. D. 1972. Neonatal bacteriuria: a prospective study in 1,460 infants. Br. Med. J.1:267-269.

2. Alexander, D. N., G. M. Ederer, and J. M. Matsen. 1976.Evaluation of an adenosine 5'-

triphosphate assay as a screening method to detect significant bacteriuria. J. Clin.

Microbiol.3:42-46.

3. Bailey, R. R. 1973. Significance of coagulase-negative Staphylococcus in urine. J. Infect.

Dis. 127:179-182.

4. Bartlett, R. C., and R. S. Galen. 1983. Predictive value of urine culture. Am. J. Clin. Pathol.

79:756-757.

5. Bartlett, R. C., D. O'Niell, and J. C. McLaughlin. 1984. Detection of bacteriuria by leukocyte

esterase, nitrite, and the AutoMicrobic System. Am. J. Clin. Pathol. 82:683-687.

6. Bruce, A. W., W. S. Chadwick, W. S. Willett, and M. 0. Shaughnessy. 1981. The role ofchlamydia in genitourinary disease. J. Urol. 126:625-629.

7. Brumfitt, W. 1965. Urinary cell counts and their value. J. Clin. Pathol. 18:550-553.

8. Brunner, H., W. Weidner, and H. G. Schiefer. 1983. Studies of the role of Ureaplasma

urealyticum and Mycoplasma hominis in prostatitis. J. Infect. Dis. 147:807-813.


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study of asymptomatic bacteriuria and sensitivity pattern of

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