student: jan Česal leader: václav Šlouf
DESCRIPTION
27 . 07. 2011. Student: Jan Česal Leader: Václav Šlouf. Effect of the Solvent Environment on Excited-State Properties of an U ntypical Carotenoid. Hu et al., PNAS 1998. S 2. t 2. Q x. t ET. S 1. Q y. t 1. S 0. S 0. Bacteriochlorophyll. Bacteriorubixanthinal. Motivation. - PowerPoint PPT PresentationTRANSCRIPT
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Student: Jan ČesalLeader: Václav Šlouf
Effect of the Solvent Environment on Excited-State Properties of an Untypical
Carotenoid
27. 07. 2011
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Motivation - earlier measurements on LH1-RC of AAP
(Aerobic Anoxygenic Phototrophic) bacteria
1
111
ETc
Bacteriorubixanthinal BacteriochlorophyllS0 S0
S2
S1
Qy
Qx
ET
Hu et al., PNAS 1998
c = 0.6 ps
ET = ?
1 = ... will be the result of this study
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Materials and methodsPump–probe spectroscopy - 110 fs- 1 kHz
Pump
Crystal
Laser system
Delay line
Light converter
Sample
Probe
Spectrograph
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Materials and methods 2
• Extraction of Bacteriorubixanthinal (Brx)- HPLC (High-performance liquid chromatography)
[min.]Time
0 10 20 30 40
[V]
Vol
tage
0,0
0,2
0,4
0,6
0,8
1,0
1,2
[%]
Com
ponents
0
20
40
60
80
2,8
1
3,2
2
3,9
3
5,5
4
7,0
5
8,0
6 8,
5
7
9,2
8
9,8
9
12,7
1
0
13,3
1
1
14,2
1
214
,7
13
15,5
1
4
17,0
1
5
18,0
1
6
19,5
1
7
22,4
1
8
23,3
1
9
24,2
2
0
25,4
2
1
27,1
2
227
,6
23
28,4
2
4
29,2
2
5
29,9
2
6
31,0
2
7
32,1
2
8
33,7
2
9
34,6
3
0
36,8
3
1
37,6
3
2
39,7
3
3
42,3
3
4
43,5
3
5
C:\ data_prep\ LCdata\Kata\BRX11-07-15-C - INT7 - 1
http://en.wikipedia.org/wiki/File:Agilent1200HPLC.jpg
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Results
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Steady-state spectroscopy
500nm
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Transient absorption
spectra
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Kinetics
ps
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Conclusions
- We measured 1
- We calculated ET 1
111
ETc
Brx BChlS0 S0
S2
S1
Qy
Qx
= 3.4 ps
ET = 0.73 ps
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Thanks for your attention