strategiesfor) cloning) how)to)ﬁnd)the)clone)for)a ... · kanr gene ecorl sites (b) ligation...

Strategies for cloning How to find the clone for a specific gene? Terms: • Direct selec:on • Different types of DNA library • Iden:fica:on from gene library • Iden:fica:on of a clone

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Page 1: Strategiesfor) cloning) How)to)ﬁnd)the)clone)for)a ... · kanR gene EcoRl sites (b) Ligation gives 13 different recombinant DNA molecules 13 different fragments Ligate into the

Strategies for cloning How to find the clone for a specific gene?

Terms: •  Direct selec:on •  Different types of DNA library •  Iden:fica:on from gene library •  Iden:fica:on of a clone

Page 2: Strategiesfor) cloning) How)to)ﬁnd)the)clone)for)a ... · kanR gene EcoRl sites (b) Ligation gives 13 different recombinant DNA molecules 13 different fragments Ligate into the

Selec:on problem!!

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Page 3: Strategiesfor) cloning) How)to)ﬁnd)the)clone)for)a ... · kanR gene EcoRl sites (b) Ligation gives 13 different recombinant DNA molecules 13 different fragments Ligate into the

Different strategies for selec:on

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Direct selec:on

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Page 5: Strategiesfor) cloning) How)to)ﬁnd)the)clone)for)a ... · kanR gene EcoRl sites (b) Ligation gives 13 different recombinant DNA molecules 13 different fragments Ligate into the

Direct selec:on for TrpA gene

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Gene library in a cosmid

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Expression of genes

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Nucleic acid hybridiza:on

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32P-‐dATP

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Labelling different techniques

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Iden:fica:on of high abundance clones

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Heterologous probing

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Southern hybridiza:on

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Immuno screening

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Ultrastructural comparison of Bonamia spp. (Haplosporidia ... · PDF fileBonamia spp., of the Phylum Haplosporidia, are ob - ligate intrahaemocytic protistan parasites, primarily

Next Generation Sequencing - UCLA CTSI · PDF fileNext gen sequencing •Steps –Obtain genomic DNA (gDNA) –Fragment the genomic DNA –Repair the ends of fragmented DNA, ligate

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Gateway pENTR Vectors - Thermo Fisher Scientifictools.thermofisher.com/content/sfs/manuals/pentr_man.pdf · pENTR™ vectors. Diagrams are provided on pages 7-11 to help you ligate

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time tradition - Augustana College · The Conversion of Saul Z. Randall Stroope (b. 1953) Sung in Latin and English Caedite, vexate, ligate vinculis! Saul! Vinculis, condemnate vexate!

THE PLACE OF SURGERY IN THE TREATMENT OF … › content › postgradmedj › 32 › 373 › 522.full.pdfcarcinoma of the cervix. Failure to ligate these vessels safely and adequately

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