strata spe sample preparation productsphx.phenomenex.com/lib/6180_i.pdfscx strong cation exchange....

Strata SPE Sample Preparation Products

• CleanExtracts• HigherRecovery• BetterReproducibility

Guaranteed Performance OurStrataSPEproductlineembodiesqualityandperformance.Itsextensivequalitycontrolprocedure

providestrouble-freesolidphaseextractionmethods.AllStrataSPEphasesandformatsaretheresultof

asophisticatedmanufacturingprocess-eliminatingvariability.

EveryproductundergoescomprehensiveQCtestingtoensurereproducibleproductmanufacturing

• Consistentflowcharacteristics

• Consistentphaseselectivity

• Inertsorbentandmaterials

Cross Reference

©2008Phenomenex,Inc.Allrightsreserved.

If Strata SPE or Sepra bulk products do not perform as well or better than your current SPE product of similar phase, mass and size, return the product with comparative data within 45 days for a FULL REFUND.

Phenomenex Strata

Waters Sep-Pak

Varian Bond Elut

Supelco Discovery UCT

JT BAKER Bakerbond

IST ISOLUTE

Macherey-Nagel Chromabond

Reversed PhaseC18-E tC18 C18 DSC-18 C18 Octadecyl C18(EC) C18ecC18-U PolarPlus

OctadecylLightloadC18 C18

C18PAHC18-T C18 DStC-18LtC8 C8 C8 DSC-8 C8 Octyl C8(EC) C8Phenyl(PH) PH DSC-PH Phenyl Phenyl PH(EC)SDB-L ENV

LMSDSC-PS/DVB H2O-phobicDVB 101 HR-P

Normal PhaseSi-1(Silica) Silica DSC-Silica Silica SilicaGel Silica SiOHFL-PR(Florisil®) Florisil® Florisil® Florisil® Florisil® FL Florisil®

NH2 NH2 NH2 DSC-NH2 AminoPropyl Amino NH2 NH2

CN CN CN-U DSC-CN CN Cyano CN CNIon ExchangeABW ENVOLUTEABNSAX SAX DSC-SAX QuaternaryAmine QuaternaryAmine SAX SBSCX SCX DSC-SCX BenzeneSulfonic

AcidAromaticSulfonicAcid

SCX SA

WCX CBA DSC-WCX CarboxylicAcid CarboxylicAcid CBA PCAScreen-C CertifyI CleanScreen

DAUNarc-2 HCX Drug

Screen-CGF CertifyHF XtractDAUScreen-A CertifyII CleanScreenTHC Narc-1 HAX

2 Phenomenex|Web:www.phenomenex.com

3

High Quality Economical Product Designed to Meet Your Sample Preparation Needs

Inert material leads to cleaner extracts Inthiscomparisontest,StrataC18-Egivescleanerextractsthanmanufacturer’salternativesolutions

Contact Phenomenex for details regarding this method.

Consistent manufacturing and QC ensures reproducible resultsRecoverydatafor3differentlotsofStrataC18-E200mg/3mL

Certificate of Analysis included with every box of Strata

NADOLOL

Ab

solu

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%

120

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60

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Lot Number1 2 3 4 5

METOPROLOL

Ab

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%

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Lot Number1 2 3 4 5

PROPRANOLOL

Ab

solu

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Lot Number1 2 3 4 5

Phenomenex Strata® C18-E

Waters® Sep-Pak® C18 IST ISoLUTE® C-18(EC) Varian® Bond Elut® C18

Improve your results now with a change to StrataCombiningover25yearsofindustryknowledgeandchromatographyexperience,PhenomenexhasdevelopedtheStrataSPElinewithinourextensiveSamplePreparationproductrange.StrataSPEencompassesavarietyofhighqualitysorbentsandformatsdesignedtoeffectivelycleanupandconcentratesamplesfrommatricesencounteredinallindustries.

Phenomenex|Web:www.phenomenex.com

4

Determining Proper Bed MassSorbent Bed Mass = Analyte Capacity (i.e.30mg,50mg,60mg,100mg,150mg,200mg,500mg,1g,2g,5g,10g,20g,50g,70g)Thesorbentbedmassdeterminesthemaximumamountofanalytethatcanberetainedbythesorbent.

Silica-based sorbents Non-ionicsorbentsretainamassofsolute(analyteplusretainedcontaminants)thatisequivalenttoapproximately5%ofthesorbentmass.Therefore,a100mgcartridgecanretainapproximately5mgoftotalsolutemass.

Ion-exchange sorbents Capacityisdeterminedbythenumberofchargedsitesonthesorbentavailabletointeractwiththeanalyte.

Suggested Loading CapacitySilica-Based Sorbents (StrataC18-E,C8,SCX,SAX,WCX,NH2,etc.)

Selecting the Right Strata SPE Product• Sorbentbedmass

• Reservoirvolume

• Wash/elutionvolume

Sample Matrix Sorbent MassBlood,serum,plasma 50mgsorbentper250µLUrine 50mgsorbentper500µLFilteredtissuehomogenates 100mgsorbentper100mgtissue

Environmental Samples Sorbent MassWater(particulate-free)drinking 500mg/100-500mLsampleWater(particulate-laden)rivers,runoff,etc.

1g/100-500mLsample

SoilExtracts 1g/100mLofsoilextract

4

Reduce breakthrough by selecting the proper bed mass!

Sorbent Wash & Elution Volumes*

ThevolumeofsolventneededforSPEprocessingisdirectlyrelatedtothemassofsorbentintheSPEtube,andmorespecifically,the“bedvolume”oftheSPEdevice.Intuitivelyweknowmoresorbentrequiresmoresolvent,lesssorbent=lesssolvent.Typically,4–16bedvolumesareusedinSPEmethods.

Reservoir = sample volume capacity(i.e.1mL,3mL,6mL,12mL,20mL,60mL,150mL)ThecolumnvolumereferstothedimensionsoftheSPEhousing(i.e.tube).Itdeterminesthelargestvolumesolventorsamplethatcanbedeliveredperaliquot.

Silica- BasedSorbent Mass

Practical Minimum Wash and Elution Volume 4 bed volumes

Recommended Wash and Elution Volume 8 bed volumes

Polymer- Based Sorbent Mass*

Practical Minimum Wash and Elution Volume 4 bed volumes

Recommended Wash and Elution Volume 8 bed volumes

10mg 60µL 120µL 10mg 100µL 200µL— — — 30mg 300µL 600µL50mg 300µL 600µL — — —— — — 60mg 600µL 1.2mL100mg 600µL 1.2mL 100mg 1mL 2mL150mg 900µL 1.8mL 150mg 1.5mL 3mL200mg 1.2mL 2.4mL 200mg 2mL 4mL500mg 3mL 6mL 500mg 5mL 10mL1g 6mL 12mL 1g 10mL 20mL2g 12mL 24mL — — —5g 30mL 60mL — — —10g 60mL 120mL — — —

*StratapolymericresinshavealargersurfaceareathanStratasilica-basedmaterialhencerequiringslightlymoresolventpergramforprocessing.Theelutionvolumesarespecifictothechemicalnatureoftheanalytebeingextracted,itsconcentrationinthesample,thechemicalnatureoftheelutingsolventandthebedmassused.Theaboveisaguideline,anelutionstudyshouldbeconductedtodeterminetheappropriatevolumetouse.


5

Selecting the Proper Strata Phase

Strata Selection Tree

Reversed Phase Benefits Typical ApplicationC18-E Extractionofhydrophobicmoleculesfromaqueousandbiologicalsamples.

Extraction of hydrophobic or polar organic analytes from

aqueous matrix

C18-U Increasedextractionefficiencyandenhancedclean-upofhydrophobiccompoundsthatcontainhydroxyoraminefunctionalgroupsfromwaterorbiologicalfluids.

C18-T Widepore.Extractionoflargehydrophobicmolecules(upto75kD)fromwaterorbiologicalmatrices.

C8 ExtractionofhydrophobiccompoundsfromwaterorbiologicalfluidsthatareretainedtoostronglyonStrataC18-EorStrata-X.

Phenyl Extractionofaromatichydrophobiccompounds.CN Extractionofnon-polar,compoundsthatareretainedtoostronglyonStrataC18-EorC8.SDB-L Styrene-divinylbenzene.Extractionofnon-polarandpolarmolecules.Normal Phase Benefits Typical ApplicationCN Normalphasesorbentthatcaneffectivelyextractpolarcompoundsfromnon-polarsolvents.

Extraction of polar analytes from non-polar organic

solvents

NH2 Extractionofstronganionsfromaqueoussamples.EPH Fractionationofaliphaticandaromaticextractablehydrocarbonsfromenvironmentalsamples.Silica Extractionofpolarcompoundsthataresimilarinstructure.Florisil®(FL-PR) Extractionofpesticidesfromenvironmentalsamples.Alumina-N(AL-N) Extractionofpolarcompoundsfromfoodandenvironmentalsamples.Eco-Screen Extractionofnaphthalenefromenvironmentalsamples.Ion-Exchange Benefits Typical ApplicationWCX Weakcationexchange.Extractionofquaternaryamines.

Extraction of charged analytes from aqueous or non-polar

organic samples

SCX Strongcationexchange.Extractionof1°,2°and3°aminesfrombiologicalfluids.Screen-C Mixedmodecationexchange.Extractionofbasicdrugsfrombiologicalmatricessuchasblood,

serumandurine.WAX Weakanionexchange.Extractionofstrongand/orweakanionsfromaqueoussolvent.SAX Stronganionexchange.Extractionoforganicacids.Screen-A Mixedmodeanionexchange.Extractionofacidicdrugsfrombiologicalmatricessuchasblood,

serumandurine.ABW Specialtyphase.Fractionationofneutralcompoundssuchasamidesfromacidicandbasic

analytes.

aqueous

water

ionic

organic

organic

neutral

organic

neutral

nonpolar

reversedphase

SDB-L

C18-E

C8

PH

(+)basic

cationexchange

SCX

WCX

Screen-C

Screen-C GF

(-)acidic

anionexchange

SAX

NH2

Screen-A

polar

reversedphase

CN

neutral

moderatelypolar

reversedphase

C8

CN

C18-U

nonpolar

reversedphase

SDB-L

C18-E

C8

PH

moderatelypolar

normalphase

Si-1

NH2

EPH

Eco-Screen

AL-N

polar

normalphase

CN

Si-1

FL-PR

NH2

(+)basic

cationexchange

SCX

WCX

Screen-C

Screen-C GF

(-)acidic

anionexchange

SAX

NH2

Screen-A

ionic


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Quality Product Offerings

Strata C18-ESorbent Mass Part No. Unit/Box PriceTube

Giga™ Tube

96-Well Plate

On-Line Extraction Cartridge

Description Part No.Unit/Box Price

Strata C8Sorbent Mass Part No. Unit/Box PriceTube

Giga™ Tube

96-Well Plate

On-Line Extraction Cartridge

Description Part No.Unit/Box Price

Strata C18-USorbent Mass Part No. Unit/Box PriceTube

96-Well Plate

Strata PhenylSorbent Mass Part No. Unit/Box PriceTube

96-Well Plate

Strata SDB-LSorbent Mass Part No. Unit/Box PriceTube

96-Well Plate

Strata CNSorbent Mass Part No. Unit/Box PriceTube

Strata C18-T (widepore)Sorbent Mass Part No. Unit/Box PriceTube

Reversed Phase

For On-line Screening• 1-3minuteruntime

• Directinjectanalysis

For Traditional Sample Preparation

•Compatiblewithmostmanifoldsandroboticworkstations

•Consistentwell-to-wellandtube-to-tubeflow

If Strata SPE or Sepra bulk products do not perform as well or better than your current SPE product of similar phase, mass and size, return the product with comparative data within 45 days for a FULL REFUND.

For Flash Analysis•Widerangeofpolarandnon-polar

selectivities

•Gramtokilogramquantities

For Flash and Large Scale-up

•Availablein12,20,60,and150mLvolumetubesizes

•Pre-packedformatseliminateexcesslaborassociatedwithglasspackedcolumns


50mg 8B-S001-DAK 1mL(100/box)100mg 8B-S001-EAK 1mL(100/box)100mg 8B-S001-EBJ 3mL(50/box)200mg 8B-S001-FBJ 3mL(50/box)500mg 8B-S001-HBJ 3mL(50/box)500mg 8B-S001-HCH 6mL(30/box)

500mg 8B-S001-HDG 12mL(20/box)2g 8B-S001-KDG 12mL(20/box)5g 8B-S001-LEG 20mL(20/box)10g 8B-S001-MFF 60mL(16/box)20g 8B-S001-VFF 60mL(16/box)50g 8B-S001-YSN 150mL(8/box)70g 8B-S001-ZSN 150mL(8/box)

25mg 8E-S001-CGB 2Plates/Box50mg 8E-S001-DGB 2Plates/Box100mg 8E-S001-EGB 2Plates/Box

StrataC18on-lineextractioncartridge,20x2.0mm

00M-S039-B0-CB

ea

Cartridgeholder,20mm

CH0-5845 ea

100mg 8B-S005-EAK 1mL(100/box)200mg 8B-S005-FBJ 3mL(50/box)500mg 8B-S005-HBJ 3mL(50/box)500mg 8B-S005-HCH 6mL(30/box)1g 8B-S005-JCH 6mL(30/box)

2g 8B-S005-KDG 12mL(20/box)5g 8B-S005-LEG 20mL(20/box)10g 8B-S005-MFF 60mL(16/box)


StrataC8on-lineextractioncartridge,20x2.0mm

00M-S101-B0-CB

ea

Cartridgeholder,20mm

CH0-5845 ea

100mg 8B-S002-EAK 1mL(100/box)200mg 8B-S002-FBJ 3mL(50/box)500mg 8B-S002-HCH 6mL(30/box)

50mg 8E-S002-DGB 2Plates/box

100mg 8B-S006-EAK 1mL(100/box)200mg 8B-S006-FBJ 3mL(50/box)500mg 8B-S006-HBJ 3mL(50/box)

50mg 8E-S006-DGB 2Plates/Box100mg 8E-S006-EGB 2Plates/Box


50mg 8E-S014-DGB 2Plates/Box

100mg 8B-S007-EAK 1mL(100/box)500mg 8B-S007-HBJ 3mL(50/box)500mg 8B-S007-HCH 6mL(30/box)


7

Strata ABW (specialtyphase)Sorbent Mass Part No. Unit/Box PriceGiga™ Tube

Strata Silica (Si-1)Sorbent Mass Part No. Unit/Box PriceTube

Giga™ Tube

96-Well Plate

Strata SCX (strongcationexchange)Sorbent Mass Part No. Unit/Box PriceTube

Giga™ Tube

96-Well Plate

Strata CNSorbent Mass Part No. Unit/Box PriceTube

Strata Florisil®

(pesticideresiduegrade)Sorbent Mass Part No. Unit/Box PriceTube

Giga™ Tube

Strata Screen-A (mixed-modeanionexchange)Sorbent Mass Part No. Unit/Box PriceTube

96-Well Plate

Strata Screen-C (mixed-modecationexchange)Sorbent Mass Part No. Unit/Box PriceTube

Strata EPH (specialtyphase)Sorbent Mass Part No. Unit/Box PriceTube

Giga™ Tube

Strata NH2 / WAXSorbent Mass Part No. Unit/Box PriceTube

Giga™ Tube

96-Well Plate

Normal PhaseStrata NH2 / WAXSorbent Mass Part No. Unit/Box PriceTube

Giga™ Tube

96-Well Plate

Anion ExchangeStrata WCX (weakcationexchange)Sorbent Mass Part No. Unit/Box PriceTube

96-Well Plate

Cation Exchange

Strata SAX (stronganionexchange)Sorbent Mass Part No. Unit/Box PriceTube

Giga™ Tube

96-Well Plate

Trademarks StrataisaregisteredtrademarkofPhenomenex,Inc.Giga,Sepra,andStrata-XaretrademarksofPhenomenex,Inc.BondElutisaregisteredtrademarkofVarian,Inc.StyreScreenisaregisteredtrademarkofUCT.Sep-Pakisaregisteredtrade-markofWatersCorp.FlorisilisaregisteredtrademarkofU.S.SilicaCo.ISOLUTEisaregisteredtrademarkofBiotage.

Disclaimer Comparativeseparationsmaynotberepresentativeofallapplications.SubjecttoPhenomenexStandardTerms&Conditions,whichmaybeviewedatwww.phenomenex.com/TermsAndConditions.

©2008Phenomenex,Inc.Allrightsreserved.


2g 8B-S030-KDG 12mL(20/box)5g 8B-S030-LEG 20mL(20/box)


500mg 8B-S012-HDG 12mL(20/box)2g 8B-S012-KDG 12mL(20/box)5g 8B-S012-LEG 20mL(20/box)10g 8B-S012-MFF 60mL(16/box)20g 8B-S012-VFF 60mL(16/box)50g 8B-S012-YSN 150mL(8/box)70g 8B-S012-ZSN 150mL(8/box)96-Well Plate100mg 8E-S012-EGB 2Plates/Box

100mg 8B-S010-EAK 1mL(100/box)100mg 8B-S010-EBJ 3mL(50/box)200mg 8B-S010-FBJ 3mL(50/box)500mg 8B-S010-HBJ 3mL(50/box)500mg 8B-S010-HCH 6mL(30/box)1g 8B-S010-JCH 6mL(30/box)

2g 8B-S010-KDG 12mL(20/box)5g 8B-S010-LEG 20mL(20/box)10g 8B-S010-MFF 60mL(16/box)20g 8B-S010-VFF 60mL(16/box)

25mg 8E-S010-CGB 2Plates/Box100mg 8E-S010-EGB 2Plates/Box

100mg 8B-S007-EAK 1mL(100/box)500mg 8B-S007-HBJ 3mL(50/box)500mg 8B-S007-HCH 6mL(30/box)

500mg 8B-S013-HBJ 3mL(50/box)500mg 8B-S013-HCH 6mL(30/box)1g 8B-S013-JCB 6mL(30/box)

2g 8B-S013-KDG 12mL(20/box)5g 8B-S013-LEG 20mL(20/box)10g 8B-S013-MFF 60mL(16/box)

100mg 8B-S019-EAK 1mL(100/box)200mg 8B-S019-FBJ 3mL(50/box)500mg 8B-S019-HCH 6mL(30/box)

25mg 8E-S019-CGB 2Plates/Box

100mg 8B-S016-EAK 1mL(100/box)100mg 8B-S016-EBJ 3mL(50/box)150mg 8B-S016-SBJ 3mL(50/box)200mg 8B-S016-FBJ 3mL(50/box)300mg 8B-S016-RBJ 3mL(50/box)150mg 8B-S016-SCH 6mL(30/box)500mg 8B-S016-HCH 6mL(30/box)

500mg 8B-S031-HBJ 3mL(50/box)

5g 8B-S031-LEG 20mL(20/box)


500mg 8B-S009-HDG 12mL(20/box)2g 8B-S009-KDG 12mL(20/box)5g 8B-S009-LEG 20mL(20/box)



500mg 8B-S009-HDG 12mL(20/box)2g 8B-S009-KDG 12mL(20/box)5g 8B-S009-LEG 20mL(20/box)




100mg 8B-S008-EAK 1mL(100/box)100mg 8B-S008-EBJ 3mL(50/box)200mg 8B-S008-FBJ 3mL(50/box)500mg 8B-S008-HBJ 3mL(50/box)500mg 8B-S008-HCH 6mL(30/box)1g 8B-S008-JCH 6mL(30/box)

500mg 8B-S008-HDG 12mL(20/box)2g 8B-S008-KDG 12mL(20/box)5g 8B-S008-LEG 20mL(20/box)10g 8B-S008-MFF 60mL(16/box)20g 8B-S008-VFF 60mL(16/box)


6180

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www.phenomenex.comPhenomenexproductsareavailableworldwide.Forthedistributorinyourcountry,contactPhenomenexUSA,[email protected]

strata spe sample preparation productsphx.phenomenex.com/lib/6180_i.pdfscx strong cation exchange....

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