sop for gcms
TRANSCRIPT
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STANDARD OPERATING PROCEDURE FOR GCMS
S.No Steps to be followed
Switch on the instrument
1 Open the Helium gas cylinder (The outlet pressure of the gas should be about
50-60 psi), Switch on the GC followed by MS. Wait for 2 minutes. Switch on the
PC
2 Double click the GCMS Real time Analysis icon
3 GCMS Real time Analysis window opens,
4 On the left side of the window there is a Assistant Bar, you click the Vaccum
control icon (If it is not present than click top icon)
5 Once we click vaccum control icon, vaccum control window opens, just click the
Auto start up.
6 Wait till the Vaccum created.
7 Once Vaccum created go back to Data acquisition window by clicking the Data
acquisition icon in Assistant bar.
8 Set the GC and MS parameter in the Data acquisition window.
9 Than save the method file.
10 Then Click the Acquisition icon followed by Download initial parameters.
11 Wait for few minutes till instrument downloads all parameters.
TUNINIG
12 If the instrument is in continuous running condition we have to do tuning after 2-
3 days interval. And if the instrument is shutdown than after restarting we have
to do the tuning every time.
13 Click on the Tuning icon present in the assistant bar
14 Click start auto tuning, Save the tuning file.
15 After completion of tuning, click the View Tuning results icon to see the tuning
results. (Check the FMHW parameter it should be in the range of 0.5 to 0.7. And
the Peak of m/z 69 should be twice than m/z 28 peak.)
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Sample Analysis
16 After setting GC and MS parameters save the method file.
17
Single sample analysis
Click on the sample Login icon, sample login window open
18 Give the name of the file, sample, select the latest tuning file, enter vial number,
injection volume, than click OK.
19 Click on the Download parameter. Run will start automatically.
20
Batch Analysis
Click the Batch processing icon, batch processing table open, enter all
parameters, Save the batch file and click the start button.
View the result of the sample
21 You can check the chromatograms during Run by clicking the snapshot icon at
Realtime Analysis window or Just go to GCMS Postrun analysis window and
open the previous data files.
22 To check the mass spectra of the particular peak, click on the peak you will get
the mass spectra.
23 To search for the library, click the required peak and than click the Similarty
search. You will get the library search result for the particular peaks.
24 To change the library goes to Qualitative, Click the similarity search parameter,
set the desired libraries. And click Ok.
25 To identify all the peaks automatically click the Integrate all the Peaks, give the
integration parameters,
26 Click the Qualitative table, Than click TIC, Click edit, select all, followed by
Registered to spectrum process table.
27 Click the Spectrum process table, Click on similarity search, search for all table,
you will get the library search result for all the integrated peaks.
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Shut Down the instrument
28 Click on Vaccum control icon, followed by Auto Shut down. Wait till auto shut
down process completed.
29 Shut down the MS, GC followed by PC and Gas.