SPE

C18

4 C18

C18

C18

C18

C18

C18

50m60

6%

SPE

Agilent Bond Elut C18LO

SIGMA-ALDRICH Spelclean LC-18

C18

C18 50m 60 6%

C18 50m 60 17%

C18 100m 60 8%

C18 50m 150 14%

SPE

Alltech AgilentSIGMA-

ALDRICHWaters

C18Bond Elut

C18 LO

Spelclean

LC-18

C18Bond Elut

C18

Discovery

DSC-18

Sep-Pak

tC18

C18

C18Bond Elut

C18-EWP

C18

Maxi-Clean

300mg 50 20926

300mg 100 20928

600mg 50 20934

600mg 100 20936

900mg 50 20942

900mg 100 20944

100g 1 211502

Extract-Clean

50mg 1.5mL 100 204900

100mg 1.5mL 100 205000

200mg 4.0mL 50 205150

500mg 4.0mL 50 205250

500mg 8.0mL 30 205350

1000mg 8.0mL 30 205430

2000mg 8.0mL 30 205450

2000mg 15mL 30 205462

5000mg 25mL 20 225450

10,000mg 78mL 16 235410

more info

Solid

Phase E

xtr

actio

n S

olid

Phase E

xtr

actio

n S

olid

Phase E

xtr

actio

n S

olid

Phase E

xtr

actio

n

SPE

C18

C18

C18

C18

50m60

17%

SPE

Agilent Bond Elut C18

SIGMA-ALDRICH Discovery DSC-18

Waters Sep-Pak tC18

Extract-Clean

100mg 1.5mL 100 255100

200mg 4.0mL 50 255200

500mg 4.0mL 50 255300

500mg 8.0mL 30 255350

1000mg 8.0mL 30 255430

2000mg 15mL 30 255440

5000mg 25mL 20 255450

10,000mg 75mL 16 255460

Maxi-Clean

300mg 50 20945

100g 1 211503

0 1 2 3 4 5 6 7 8 9 10 Min.

12 3

4

5

6

78

After SPE Concentration

Before SPE Concentration

Column: Alltima C18, 3m, 100 x 4.6mm HPLC Column (Part No. 81382)Mobile Phase: A: 25mM Ammonium Acetate, 0.1%TFA B: Acetonitrile, 0.1%TFAGradient: (Time, %B): (0,20,), (10,90)Flow Rate: 1.0mL/minDetector: ELSD

1. Acetazolamide2. Hydrochlorothiazide3. 4. Clopamide5. Trichloromethiazide6. Indapamide7. 8. Spironolactone

1028910290Extract-Clean C181000mg

8

1.25g/mL

5mL 5mL

15mL 1mL/

5mL

2mLHPLC

250L

0 1

1

2 3 Min.

Column: Alltima C18, 3m 53 x 7mm Rocket HPLC Column, (Part No. 50605)Mobile Phase: ACN:20mM K2HPO4, pH 7.3 (65:35)Flow Rate: 3mL/minDetector: UV 340nmInj. Vol.: 5L

9133

1. Apigenin-7-Glucoside

Extract-Clean C18500mg

1mg

50:506mL 20mL

5mL 5mL

1mL

1mL

50:502mL

2mL

more info

Solid

Phase E

xtr

actio

n S

olid

Phase E

xtr

actio

n S

olid

Phase E

xtr

actio

n S

olid

Phase E

xtr

actio

n

SPE

C18

C18

0 5 10 15 20 25 30 35 Min.

Before SPE Concentration

1

2

3

45

6

After SPE Concentration

Column: Adsorbosphere UHS C18, 5m, 150 x 4.6mm HPLC Column (Part No. 288118)Mobile Phase: Methanol:Water (50:50)Flow Rate: 1.0mL/minDetector: UV at 254nmTemperature: Ambient

1. 2,4-Dinitrotoluene2. 1-Naphthol3. 2-Naphthol4. 2-Nitrotoluene5. 4-Nitrotoluene6. 3-Nitrotoluene

1059710598Extract-Clean C18500mg

100g 7.5g/g

1000mL10

5mL 5mL

1000mL 1~5mL/

15

50501mL x 3

15

0 10 20 30 35 40 45 50 605 15 25 55 65 Min.

Before SPE Clean-up

1

2

6

34

5

After SPE Clean-up

1. Carbendazim2. Thiabendazole3. Triadimenol4. Triadimefon5. Flusilazole6. Diniconazole

1063710638

Column: Alltima HP C18 Amide, 5m, 250 x 4.6mm HPLC Column (Part No. 87734)Mobile Phase: A: Water B: AcetonitrileGradient: (Time, %B): (0,15%), (15,45%), (50,45%), (65,15%)Flow Rate: 1mL/minDetector: UV at 254nm

Extract-Clean C18500mg

0.167mg/mL

1mL 1mL

3mL 3mL

2mL

3mL

0 1

1

2

2

3

3

4

4

5

5

1. Methysticin2. Yangonin3. Kavain4. Dihydrokavain5. Dihydromethysticin

9125

Column: Alltima C18, 3m, 53 x 7mm Rocket HPLC Column (Part No. 50605)Mobile Phase: ACN:IPA:0.2% Acetic Acid (17:23:60)Flow Rate: 3.5mL/minDetector: UV 220nmInj. Vol.: 5L

Extract-Clean C181000mg

300mg

80202mL x 48mL

10mL

5mL 5mL

2mL

2mL

3mL

0 1

1

2 3 4 5 Min.

9132

1. Z-ligustilide

Column: Alltima C18, 3m 53 x 7mm Rocket HPLC Column (Part No. 50605)Mobile Phase: Methanol:0.2% Acetic Acid (65:35)Flow Rate: 4.5mL/minDetector: UV 270nmInj. Vol.: 5L

Extract-Clean C181000mg

1500mg

5mL x 3 15mL

30mL

5mL 5mL

1mL

2mL

2mL

Solid

Phase E

xtr

actio

n S

olid

Phase E

xtr

actio

n S

olid

Phase E

xtr

actio

n S

olid

Phase E

xtr

actio

n

SPE

C18

C18

Extract-Clean C18

500mL2-75mL

PAH 2000g/mL 20L 40g/500mL

PAH 80ppb

5mL x 22-

85155mL

210705 75mL

210575

30

CH 2Cl2 1mL x 3 PAH

1mL

Extract-Clean C18

500mL2-75mL

PAH 2000g/mL 20L 40g/500mL

PAH 80ppb

5mL x 22-

85155mL

210705 75mL

210575

30

8515500L x 2

10

CH 2Cl2 1mL x 3 PAH

1mL

PAH

PAH

Solid

Phase E

xtr

actio

n S

olid

Phase E

xtr

actio

n S

olid

Phase E

xtr

actio

n S

olid

Phase E

xtr

actio

n

SPE

C18

C18

C18

C18

C18

100m60

8%

C18

C18

50m150

14%

EPA36203610

1500MW

SPE

Agilent Bond Elut C18-EWP

Extract-Clean

500mg 4.0mL 50 215250

1000mg 8.0mL 30 215430

Maxi-Clean

300mg 100 22012

600mg 100 22017

900mg 100 220215

100g 1 211542

0 5 10 15 20 25 Min.

Before SPE Concentration

6

5

4

321

After SPE Concentration

1. Aldicarb sulfoxide2. Methomyl3. 3-Hydoxycarbofuran4. Propoxur5. Carbaryl6. Methiocarb

Column: Platinum EPS C18, 5m, 250 x 4.6mm HPLC Column (Part No. 32246)Mobile Phase: A: DI water B: AcetonitrileGradient: (Time, %B): (0,25,), (5,25), (20,50), (25,50), (30,25)Flow Rate: 1mL/minDetector: UV at 210nmTemperature: Ambient

1059910600Extract-Clean C18

500mL 125L

25ppb

8020 3mL

500L

3mL x 2

80201mL x 4

more info

more info

Solid

Phase E

xtr

actio

n S

olid

Phase E

xtr

actio

n S

olid

Phase E

xtr

actio

n S

olid

Phase E

xtr

actio

n

C18sC18sC18sC18sC18s.pdf