-
SPE
C18
4 C18
C18
C18
C18
C18
C18
50m60
6%
SPE
Agilent Bond Elut C18LO
SIGMA-ALDRICH Spelclean LC-18
C18
C18 50m 60 6%
C18 50m 60 17%
C18 100m 60 8%
C18 50m 150 14%
SPE
Alltech AgilentSIGMA-
ALDRICHWaters
C18Bond Elut
C18 LO
Spelclean
LC-18
C18Bond Elut
C18
Discovery
DSC-18
Sep-Pak
tC18
C18
C18Bond Elut
C18-EWP
C18
Maxi-Clean
300mg 50 20926
300mg 100 20928
600mg 50 20934
600mg 100 20936
900mg 50 20942
900mg 100 20944
100g 1 211502
Extract-Clean
50mg 1.5mL 100 204900
100mg 1.5mL 100 205000
200mg 4.0mL 50 205150
500mg 4.0mL 50 205250
500mg 8.0mL 30 205350
1000mg 8.0mL 30 205430
2000mg 8.0mL 30 205450
2000mg 15mL 30 205462
5000mg 25mL 20 225450
10,000mg 78mL 16 235410
more info
Solid
Phase E
xtr
actio
n S
olid
Phase E
xtr
actio
n S
olid
Phase E
xtr
actio
n S
olid
Phase E
xtr
actio
n
-
SPE
C18
C18
C18
C18
50m60
17%
SPE
Agilent Bond Elut C18
SIGMA-ALDRICH Discovery DSC-18
Waters Sep-Pak tC18
Extract-Clean
100mg 1.5mL 100 255100
200mg 4.0mL 50 255200
500mg 4.0mL 50 255300
500mg 8.0mL 30 255350
1000mg 8.0mL 30 255430
2000mg 15mL 30 255440
5000mg 25mL 20 255450
10,000mg 75mL 16 255460
Maxi-Clean
300mg 50 20945
100g 1 211503
0 1 2 3 4 5 6 7 8 9 10 Min.
12 3
4
5
6
78
After SPE Concentration
Before SPE Concentration
Column: Alltima C18, 3m, 100 x 4.6mm HPLC Column (Part No. 81382)Mobile Phase: A: 25mM Ammonium Acetate, 0.1%TFA B: Acetonitrile, 0.1%TFAGradient: (Time, %B): (0,20,), (10,90)Flow Rate: 1.0mL/minDetector: ELSD
1. Acetazolamide2. Hydrochlorothiazide3. 4. Clopamide5. Trichloromethiazide6. Indapamide7. 8. Spironolactone
1028910290Extract-Clean C181000mg
8
1.25g/mL
5mL 5mL
15mL 1mL/
5mL
2mLHPLC
250L
0 1
1
2 3 Min.
Column: Alltima C18, 3m 53 x 7mm Rocket HPLC Column, (Part No. 50605)Mobile Phase: ACN:20mM K2HPO4, pH 7.3 (65:35)Flow Rate: 3mL/minDetector: UV 340nmInj. Vol.: 5L
9133
1. Apigenin-7-Glucoside
Extract-Clean C18500mg
1mg
50:506mL 20mL
5mL 5mL
1mL
1mL
50:502mL
2mL
more info
Solid
Phase E
xtr
actio
n S
olid
Phase E
xtr
actio
n S
olid
Phase E
xtr
actio
n S
olid
Phase E
xtr
actio
n
-
SPE
C18
C18
0 5 10 15 20 25 30 35 Min.
Before SPE Concentration
1
2
3
45
6
After SPE Concentration
Column: Adsorbosphere UHS C18, 5m, 150 x 4.6mm HPLC Column (Part No. 288118)Mobile Phase: Methanol:Water (50:50)Flow Rate: 1.0mL/minDetector: UV at 254nmTemperature: Ambient
1. 2,4-Dinitrotoluene2. 1-Naphthol3. 2-Naphthol4. 2-Nitrotoluene5. 4-Nitrotoluene6. 3-Nitrotoluene
1059710598Extract-Clean C18500mg
100g 7.5g/g
1000mL10
5mL 5mL
1000mL 1~5mL/
15
50501mL x 3
15
0 10 20 30 35 40 45 50 605 15 25 55 65 Min.
Before SPE Clean-up
1
2
6
34
5
After SPE Clean-up
1. Carbendazim2. Thiabendazole3. Triadimenol4. Triadimefon5. Flusilazole6. Diniconazole
1063710638
Column: Alltima HP C18 Amide, 5m, 250 x 4.6mm HPLC Column (Part No. 87734)Mobile Phase: A: Water B: AcetonitrileGradient: (Time, %B): (0,15%), (15,45%), (50,45%), (65,15%)Flow Rate: 1mL/minDetector: UV at 254nm
Extract-Clean C18500mg
0.167mg/mL
1mL 1mL
3mL 3mL
2mL
3mL
0 1
1
2
2
3
3
4
4
5
5
1. Methysticin2. Yangonin3. Kavain4. Dihydrokavain5. Dihydromethysticin
9125
Column: Alltima C18, 3m, 53 x 7mm Rocket HPLC Column (Part No. 50605)Mobile Phase: ACN:IPA:0.2% Acetic Acid (17:23:60)Flow Rate: 3.5mL/minDetector: UV 220nmInj. Vol.: 5L
Extract-Clean C181000mg
300mg
80202mL x 48mL
10mL
5mL 5mL
2mL
2mL
3mL
0 1
1
2 3 4 5 Min.
9132
1. Z-ligustilide
Column: Alltima C18, 3m 53 x 7mm Rocket HPLC Column (Part No. 50605)Mobile Phase: Methanol:0.2% Acetic Acid (65:35)Flow Rate: 4.5mL/minDetector: UV 270nmInj. Vol.: 5L
Extract-Clean C181000mg
1500mg
5mL x 3 15mL
30mL
5mL 5mL
1mL
2mL
2mL
Solid
Phase E
xtr
actio
n S
olid
Phase E
xtr
actio
n S
olid
Phase E
xtr
actio
n S
olid
Phase E
xtr
actio
n
-
SPE
C18
C18
Extract-Clean C18
500mL2-75mL
PAH 2000g/mL 20L 40g/500mL
PAH 80ppb
5mL x 22-
85155mL
210705 75mL
210575
30
CH 2Cl2 1mL x 3 PAH
1mL
Extract-Clean C18
500mL2-75mL
PAH 2000g/mL 20L 40g/500mL
PAH 80ppb
5mL x 22-
85155mL
210705 75mL
210575
30
8515500L x 2
10
CH 2Cl2 1mL x 3 PAH
1mL
PAH
PAH
Solid
Phase E
xtr
actio
n S
olid
Phase E
xtr
actio
n S
olid
Phase E
xtr
actio
n S
olid
Phase E
xtr
actio
n
-
SPE
C18
C18
C18
C18
C18
100m60
8%
C18
C18
50m150
14%
EPA36203610
1500MW
SPE
Agilent Bond Elut C18-EWP
Extract-Clean
500mg 4.0mL 50 215250
1000mg 8.0mL 30 215430
Maxi-Clean
300mg 100 22012
600mg 100 22017
900mg 100 220215
100g 1 211542
0 5 10 15 20 25 Min.
Before SPE Concentration
6
5
4
321
After SPE Concentration
1. Aldicarb sulfoxide2. Methomyl3. 3-Hydoxycarbofuran4. Propoxur5. Carbaryl6. Methiocarb
Column: Platinum EPS C18, 5m, 250 x 4.6mm HPLC Column (Part No. 32246)Mobile Phase: A: DI water B: AcetonitrileGradient: (Time, %B): (0,25,), (5,25), (20,50), (25,50), (30,25)Flow Rate: 1mL/minDetector: UV at 210nmTemperature: Ambient
1059910600Extract-Clean C18
500mL 125L
25ppb
8020 3mL
500L
3mL x 2
80201mL x 4
more info
more info
Solid
Phase E
xtr
actio
n S
olid
Phase E
xtr
actio
n S
olid
Phase E
xtr
actio
n S
olid
Phase E
xtr
actio
n
C18sC18sC18sC18sC18s.pdf
